Document pm1ze8Rm0VVb2y2aYanqyLngE

ACUTE TOXICITY TO AQUATIC INVERTEBRATES TEST SU STANCE (EASTERN OYSTER) Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfoniacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted. The testsample isFC-206, identifiebdy the laboratoryas "Sample C". Currentinformation indicatesitisa mixtureof0.67% PFOS, 17.5% diethyleneglycolbutylether, 78.91% water,1.33% Sultonefoamer, 1% sodium octylsulfate0,.04% sodium laurylsulfate0,.5% polyoxyethylenemonooctylphenylether,and 0.05% benzotriazole. The followingsummary applies toa mixture with incompletely characterized concentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: Measurement ofWater Qualitywiththe PacificOyster Bioassay, C.E. Woelke, 1967, ASTM Spec. Tech. Publ.416 Type: Acute static GLP: No Year completed: 1974 Species: Crassostreavirginica Supplier:Spawning inthelaboratoroyffield-collecatdeudltsfrom Milford, CT harbor. Analyticalmonitoring: Salinity. Exposure period: 48-hours Test organism age: Fertilizeedggs Statisticamlethod: TL50 (median tolerancelimitv)aluescalculatedusinga linearregressionequation. Test conditions: Dilutionwater: Filteresdeawater pumped from Milford,CT and treatedwithultraviolleitghtbeforeuse. Dilutionwater chemistry: Salinity: 26 - 28 ppt Lighting: Not given. harbor Stock and testsolutionpreparation: Directadditionbased on weight/volume. Exposure vessels: 500 mL beakers containing300 mL oftestsolution. Number of replicates:3 Number of organisms: Approx 150,000 embryos/L Number of concentrations: nine plusa blankcontrol Elementbasis: Number ofnormalldyevelopeldarva(estraight-hinged veligerstage) Water chemistry during the study:Not given. RESULTS Nominal concentrations:Blankcontrol1,0,24,49, 75, 100, 240,490, 750, and 1000 mg/L. Element values: 48-hourTL50 > 100 <240 mg/L Element values based on nominalconcentrations Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-206 48-hour TL50was determined to be >100 and <240 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul, Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. Testinglacksdescriptionand complete recordofmethodology used. The sample puritywas not properly characterizedand the studylacksanalyticaclonfirmatioonfthe amount of fluorochemicalproportioninthe solution. REFERENCES Test was conducted by Bionomics,EG&G, Inc.,Wareham, MA atthe request ofthe 3M Company, St.Paul,MN, 1974. OTHER Last changed: 6/28/00 BIOASSAY REPORT SUBMITTED TO 3 M COMPANY ST. PAUL, MINNESOTA ACUTE TOXICITY OF SAMPLE C TO,ATLANTIC (Crassostrea virginica). OYSTER Bionomics E G & G., Inc. Environmental Consultants 790 Main Street Wareham, Massachusetts July, 1974 This investigation was performed at the aquatic toxicology laboratory of Bionomics, E G & G, Inc., in Wareham, Massachusetts through the cooperation of the U. S. Bureau of Commercial Fisheries Shellfish Research Laboratory in Milford, Connecticut. The susceptibility of the oysters to Sample C, a light brown liquid, which was tested as 100% active, was measured in terms of the 48-hour tolerance limit (TL 50)0 the concentrat-ion of the chemical in water which causes 50 percent response under the test conditions during a 48-hour interval. The response observed in these studies was normal embryonic development. For observations on development of embryos, fertilized eggs were introduced into the test container soon after release and fertilization, usually when the eggs were in the two-cell stage of development. Quantitative samples were taken 48 hours later to determine the percentage of the fertilized eggs that had developed to a normal morphological stage (i.e. straight-hinged veliger 1.arva.e). The pred-Action of a TL 50 value, and its 95% confidence interval, was based on the conversion of.the concentrations tested and the corresponding observed percent normal development to logs and probits respectively, and the subsequent mathematical calculation of a linear regression equation. Page two The test pr ocedures used in these evaluations are those described by Woelke 1 for the measurement of water quality with the Pacific oyster embryo bioassay. Sexually mature Atlantic oysters were collected from Milford harbor and held at the BCF Shellfish Laboratory in filtered sea water for 7 days at a temperature of 220 C. Several hours prior to starting a bioassay about ten (10) mature oysters are placed in a pyrex tray filled with ultraviolet-light-treated water. -About 30 minutes before spawning is desired, the water temperature is 0 raised to 30,C and a sperm suspension from a sexually mature, sacrificed male oyster is added to the water. The combination of increased temperature and sperm induces one or more of the female oysters to spawn. Eggs from a single female are selected for use in the bioassay and the number of eggs/unit volume are determined by sampling the sperm-egg suspension. The bioassay was conducted using 500 ml beakers contairi7ing300 ml of filtered sea water (treated with ultraviolet light) having a salinity of 26-28 O/oo; each unit was inoculated with a sufficient amount of egg suspension to give approximately 150,000 fertilized eggs per liter. 1 Woelke, C. E. - "14easurement of Water Quality with the Pacific* Oyster Bioassay, "Water Quality Criteria, ASTM Spec. Tech. Publ. 416, Am. Soc. Testing Mats, 1967, P. 112-120. Page three Sample C was tested at nominal concentrations ranging from 1000.0 to 10.0 mg/l, with triplicate cultures inoculated and incubated at each concentration. The cultures were incubated for a period of 48 hours at 25 OC. At the end of this period cultures were poured through a 37,u sieve to obtain samples containing about 200 larvae and preserved in 5% formalin for microscopic examination. The number of normal and abnormal larvae were counted in each sample with the values from the triplicates being averaged, The predicted 48-hour TL5o (i.e., the concentration which inhibited normal development of 50% of the developing oyster larvae) was >100.0 <240.0 mg/l of Sample C. Table 1 presents the concentrations of Sample C tested and corresponding observed percent normal development after 48 hours of exposure. This data should be evaluated with the knowledge that errors involved in the above techniques for determining numbers of larvae developing from fertilized eggs to straight-hinge larvae have been fou-ndto be about 10 percent .2 2Davis, H. C. and H. Hidu. 1969. Effects of pesticides on embryonic development of clams and oysters and on survival and growth of the larvae. U. S. Dept. Interior, Bureau of Commercial Fisheries, Fishery Bulletin 67 (2): 393-404. SUBMITTED BY: Bionomics E G & G, Inc. Environmental Consultants 790 Main Street Wareham, Massachusetts July, 1974 PREPARED BY: APPROVED BY: Robert E. Bentley Afqulaattiicc Biologist Bevier Hasbrouck Sleight, III Director, Freshwater Programs Table I Concentrations tested and corresponding percent observed normal development for Atlantic oyster larvae (Crassostrea virp-inica)-exposed to Sample C for 48 hours. Concentration (mg active ingredient/liter) Observed percent normal develodmenta 48-hours 1000.0 750.0 490.0 240.0 100.0 75.0 49.0 24.0 10.0 control 0 0 0 0 5 90 1> 90 ti,90 > 90 >90 > 90 a Each percent is an average of triplicate values from each concentration.