Document pjRZaa71vd41Y1d5kYyGpEkB
&x6
ACUTE TO XICITY TO FISH (FATHEAD MINNOW)
TEST SUBSTANCE_______________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FX-3103. (1Octanesulfonic acid) (CAS # 2795-39-3).
Rem arks: The 3M production lot number was not noted. The test sample is FX-3103, 3%. Current information indicates it is a mixture of 0.96% PFOS, 20% diethylene glycol butyl ether, 50.56% water, 15% ethylene glycol, 4.32% sodium octyl sulfate, 6.0% polyoxyethylene monooctylphenyl ether, 2.5% propane sultone foamer, 0.5% sodium nitrate, 0.06% sodium lauryl sulfate, and 0.1% benzotriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD:________________________________________________________
Method: Not given.
Type: Acute static-renewal range-finder
GLP: No
Y ear com pleted: 1985
Species: Pimephales promelas
Supplier: Commenai Supplier, Brady, NE.
A nalytical m onitoring: Temperature, pH, and dissolved oxygen.
Exposure period: 96-hours
S tatistical methods: LC50values were calculated using logistic regression
methods (probit analysis).
Test fish age: Juveniles
Length and weight:
Average length = 41 mm
Average weight = 0.50 g (wet)
Loading: 0.67 g/L
Pretreatm ent: 25 mg/L Tetracycline HCI
Test conditions:
Dilution w ater: Carbon-filtered well water
Dilution w ater chem istry: Not given.
Lighting: Not given. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Solution renewal during the study. Stability o f the test chem ical solutions: Not noted. Exposure vessels: 4 L glass beakers containing 3 L test solution. Num ber o f replicates: 1 for exposure concentrations, 2 for blank control. Num ber o ffis h per replicate: 4.
002961
Num ber o f concentrations: five plus a blank control W ater chem istry during the study:
pH range (0-96 hours): 7.6 - 7.8 (control exposure) 7.7 - 7.8 (1000 mg/L exposure)
Tem perature range (0-96 hours): 19 - 20 C Dissolved oxygen range (0-96 hours):
7.1 - 9.3 mg/L (control exposure) 5.9 - 9.3 mg/L (1000 mg/L exposure)
RESULTS________________________________________________________
Nom inal concentrations: Blank control, 1 ,1 0 ,1 0 0 , 500, and 1000 mg/L
Elem ent values: 96-hour LC50 = >1000 mg/L
Element values based on nominal concentrations
Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
C O N C L U S IO N S ___________________________________________________
The test sample 96-hour LC50for fathead minnow was determined to be >1000 mg/L.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
R eliability: Klimisch ranking 3. The study was a range-finder with limited number of organisms exposed at each concentration. There is a lack of characterization of the test sample. Test solutions lack analytical confirmation of the amount of fluorochemical proportion in the solution.
R E F E R E N C E S ____________________________________________________
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number C 1111-1, 1985.
OTHER__________________________________ ,_______________________
Last changed: 6/26/00
002962
AQUATI C')TOXrCI TY^m T^S ffEETV exploratory ( range- finder)
.,
lr '.'/'S ' -- /
'pe Tie s t ^ < ^ 2
->*7/7^ Material Tested
7a t O g a n W ^ ^ ^ l a , ) Source. ^ ^
y//3 <
Ix3t_______________
Date Rec-d ^ g ^ j ^ / B a t e h
^/, //g.Wt^T, <y9 grams Avg. Size
cm No. Organisms per dose
Loading Factor
g/1
Lsh Disease Treatm ent:
f/h-yfty
ast Solution Volume
Liters/All-Glass Tank Tank Dimensions: 4 -liter Pyrex^ $1ass beaker
s 'A Z / fiS '*" Started___/ j y r ^ Date Started
Date Ocepleted
/Llution Water carbon-filtered well water (Temp.
-<35C., D.O. *7
p p . F
Y ,y
^ ^ ^ 2 ^ = 5 0 <95i ODnfMence
f^<X
rank I n i t i a l T o ta l |
24 Hours
48 Hours
72 Hours
96 Hours
|
*>. Cone. Amount T o ta l No. PH D.O. T o ta l No. pH D.O. T o ta l No. PH D.O. T o ta l No. pH D.O.
mg/1 Added
Dead
ppm Dead
ppn Dead
ppm Dead
ppn
+^VWt|
35~ Ctontro]
o dk X 5 n t i YA n J 1Y >
ifi
2 L C otcW i'
0 m "ftk? 0 7<?-<%(1 a tL 11 n t2 =?
^J_ 1
r.' %(kS o
n 71 n ZZfas'
zZj. 1 0
!O o
dt
c$or> K-SOO
0 o
Q
n
Ik faff n n
Tfi k l r >
i(h fad o...
mw n
11'tfif n 11M . o mfad 0
U F TM 11 t**LTfid 2 2 fnd
a S . in n ro S o o n
a
tL
o m S3 n it bl) n tL 1 7 ?
T e st Tenc. C I n it ia ls & Date
Ljn n e n ts : 4
11
/7cW
rpfj (f
JO*
t^zn #0-
-^AS/Y/FfS-
i It'.
-<
aoondary E ffe c ts (o th e r than M o r ta lity ) :
v
BC50_
BE6i ounrAVAILAfi
002963
CHEMICAL CONCENTRATION (mg/1)
ENVIRONMENTAL LABORATORY
TABLE I. SUMMARY OF ACUTE TOXICITY OF
TO FATHEAD MINNOW UND
h a r /M l .EXPOSURE CONDITIONS( 1)
24-HR.
PERCENT MORTALITY
48-HR.
72-HR. __
CONTROL _ 1 _____
-LO IQO___
\OO
o
\
o
o
o
o
o
O
O
6 o
o
o
o o o
C
o o
'ooN
96-HR.
a o
o
o
o o
LC,-_ (95Z CONFIDENCE LIMITS)(2)
50 * g / l
>)0 0 Q
>1000
>/000
(1) Data were averages of two replications for each concentration with ten (10) fish per replicate* A total of twenty fish were used per concentration.
( 2) 10jq values were calculated using logistic regression methods (Probit Analysis).
>1000
