Document pgMm9rdyQNVB8ZegbqYpVrQa
AR 826 - 0769
MICROBICS MICROTOX TOXICITY TEST
IesTsuBsSTANCE
0000
Identity: Mixture containing perfluorooctanesulfonate which may also be
referred to as PFOS, FC-85, or as a component of FC-203FC. aOctanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample
is FC-203FC. Current information indicates it is a mixtureof 0.85% PFOS, 17% diethylene glycol butyl ether, 74.46% water, 2.34% acrylic foamer, 1.7% alkyl ether sulfate, 2.55% sodium octyl sulfate, 0.12% sodium nitrite, 0.09% sodium molybdate, 0.85% triethanolamine, and 0.04% tolyltriazole.
The following summary applies to a mixture with incompletely
characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample.
M0 ETH0 OD: 0000000000 00
Method: Microbics Microtox "BASIC" Procedure
GLP: No
Year Completed: 1997
Species: Photobacterium phosphoreum
Analytical monitoring: pH and osmotic condition.
Exposure period: 30-minutes
`Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox
Test organism source: Microbics' Reagent.
Test conditions:
Diluent: Millipore Milli-QTM water
Test temperature: Not noted.
Stock and test solution preparation: A 2 g/L primary stock solution was prepared by dissolving 200 mg of test substance in 100 mL of
Millipore Milli-Q" water. The pH of the stock solution was 6.6. An
osmotic adjustment was made using 200 mg NaCl dissolved in 10 mL.
of stock solution. Appearance of test solutions was noted as "clear".
All test solutions were made by proportional dilutions.
Exposure vessels: 4 mL cuvettes.
Number of replicates: two
Number of concentrations: Four plus a blank control
rn
Element basis: Percent light loss
R0 ESUL0 TS 00000000000
Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L.
001339
Element value:
5-minute 15-minute
ECso ECso
= =
604 444
(572 (423
-- --
637) 466)
mg/L mg/L
30-minute ECso = 363 (349 - 376) mg/L
Element values based on nominal concentrations.
SRuebmsatraknsc:e TReesmtianrgkwsafsieldc.onTdhucetveadlouenstrheepmoirxtteudraepapslydetosctrhiatbemdixitn utrhee aTnedstnot the fluorochemical proportion alone.
Co0 nclusions 000
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mglL.
:
PSauublm,itMtNe,r:5531M33Company, Environmental Laboratory, P.O. Box 33331, St.
p0 ata0 uaur0 y 000000000
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lacks analytical solution.
confirmation
of
the
amountof
fluorochemical
proportion
in
the
R0 EFERENCES 000
This Lab
RsetquudeyswtasnucmobnedrucRt1e3d35b,y
the 3M 1997.
Environmental
Laboratory,
St.
Paul,
MN,
O0 THER 00000 Last changed: 6/26/00.
001340
i ENVIRONMENTAL LABORATORY: MICROTOX PREP SHEET |
LR: R 1335
Datei317-7
Analyst: JS
TEST SUBSTANCE: Fe-2035 AFEE
= lef 7
--------------------------------
1. PrepaofrStaocktSoiluotinon
)
}
+ Mass/Vol. TestSubstance: * FinalDilution Volume:
300mg| "2.o\ 0
100m|
i{ i
*Diluent: pW\[\-CD Era
i
*FinalConcentration of StockSolution: 2qle
-
=z
2. pHAdjusofSttomckSeolnutiton
*Initial pH ofStockSolution:_G -
*pHAdjusted to:
=
* pH Adjustedwith:
==
3.Osmotic Adjuosf SttomckSeolnutiton
* Osmotic Adjuster:
NaCl
*Comments: Jocnl of Sioris sal.
dion wasaddeddo
:
i
i
i
cig Nell
4. AppearanceofpH andOsmoticallyAdjustedStockSolution
5
ECLERYr OPAQUE (circleone)
'
*Color:
Lleqc
* Comments: --ieeee----------------------J --
5. Comments
rT
_--m-e--eeee,e,---------------- --_--_--m----TM--_--
001341
Pg ref
FILE: R1335.K5
MICROTBOaXsicDATTAostREPORT
FC-203FC AFF 3-17-97 TRE
Text Tims: b minutes
Osmotic:
NOMRRK
GSooaniteeaall
TO/rT
9985..3361,7 8946.0.5
CONC
CR/GAMMA
n0.o0
40onagg60s0 &
Adjustment Nai :
~~ % EFFECT
AV % DIFF.
hoy
231 3999531...449447777 617343.00465%28 2118220550..000000000000 0[0..S13E41H32 i5 59571.136177 6a370.z629 26000.l00000000 00.l3B8050 #4 7A8 98A944.1l94s625/// 423460..0820565 11500000000..0000000000000 101...878058730 8#& CB:UsidCoGfOorRRH~CaTlToOuNlatFiAoCnTsOR = 0.8933 BC50 603.5 me/L (95% CONFIDENCE RANGE:STL.5 70
21553.583 2464..70 48452...419 837.5
19 ga< 25.3 1p
43.7 q 0.8
64.8 40.5
J
Signature
ery
ast pate: 32/797.
:
PMR cans. owieone
CONTINUED TO NEXT PAGE...
-
001342
FILE:RI335.Ks PAGE 2
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JSLOPE = 1.1797
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ESTIMATING FQUATION: LOG C = 0.8450 x LOG bh +2. 7807
C9O5%RFCFOTNCFIIEDNETNCOEF FDAECTTRORRM:INAT1I.O0N5:599R2 FO=R E0C.5909676
001343
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Fett Cake FILE: R1335.Ki% sare mTs n TA MLICROTOX DATA REPORT
-- Test Time: torte 15 minute: CCointnraa) S 96.31S7 7T9.00
CONG. GAMMA Osmotic x ERECT AV % Adjustment:NaCl u pre
0a0o Doasmzeaws
TTT
ooWavan i
88.44/ 65.75
2
93.87/ 65.77
T 545 Om99M75..e3116Es// 36e53R..s5958 aan 17s
hae Liz5. 0000
125.0000
efeoee 520500..00000000 lonolaoss
Sood Gory CR = CoCORRECTIUN TactFACTOR = 0.8289
fim, 0.241
0.183
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Ine19.4
15.5
17.4 g 2.0
MOMh LsLoLo 5331..19
31.7 1.4
EC50 443.7 mg/L (95% CONFIDENCE RANGE:422.7 TO 465.7)
------
ftps
CONTINUED TO NEXT PAGE...
Test Date: 37/7597
TIME: __
----r-w
001344
FILE:R1335.K16 PAGE 2
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1000 CONCENTRATION 10E4
10E5
E95S%TIMCAOTNIFNIGDENECQUEATFIAOCNT:OR:LOG 1G.04=9608.739F1ORxECLSO5G0 b +2.6471 COEFFICTENT OF DETERMINATION: R2 = 0.99635
001345
.
rye of
FFICL-E2:038RC1335.AKR3E0F 4-17 Test Time: 30 minutes
NUMBER
To/1T
CCoonnttrrooll 9965..3381,/ 7733..0714
Basic Test MICROTOX DATA REPORT 47 1Ks
Osmotic
CONC.
CR/GAMMA
00.00
00..77665830 &#
Adjustment:NaCl
% EFFECT AV % -
g DIFF. Te
1
98.44/ 60.31
125.0000
32
9933..4847// 4558..7448 215205..00000000
e45 7
9849597.1.193661/// 94142/
24217671...179331930
525050000..000000000000 100000000
8 84165; 12135 100000000
C8R Us=edCoCfOoRrRcEaClTcIuOlNatFiAoCnTsOR = 0.7656
0.250 #
00..526249 4#
011...56497895848# 44..000415 8&
20.0
3168..16 19.3 9 '0.7
536963...805 holo
36.3 q 0.2
61.4 41.6
Bolo 80.3 go 0.1
EC50 362.6 ma/l. (45% CONFIDENCE RANGE: 349.1 TO 378.5)
Signature
pa
CONTINUED TO NEXT PAGE...
18ST TpaHrEe:: 3/7p9--7
001346
.
.
FILE:R1335.K30
PAGE 2
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EabS%TIMCAOTNIFNIGDENECQEUATFIAOCNT:OR:LOG1C.03=8501.728F3ORxELCO5G0 b +2.5594 COEFFICIENT OF DETERMINATION: R'z = 0.99762
001347