Document peqOp9avJa3yG1djRxO7XkbBd

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfonaiccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 30034. The testsample is FC-203CF. Currentinformatioinndicateistisa mixtureof 1% PFOS, 20% diethyleneglycolmonobutylether,70.2% water,2.75% Acrylicfoamer,3% sodium octylsulfate1,% triethanolamin2e%, sulfonatedalkylethersalts,and 0.05% tolyltriazole. The followingsummaty appliesto a mixture with incompletely characterizedconcentrationsof impurities.Data may not accurately reflecttoxicityof the fluorochemicalcomponent of the testsample. METHOD: Method: Standard Guide forConductingAcute ToxicitTyests withFishes, Macroinvertebratesa,nd Amphibians (ASTM E729-88a),1995 Type: Acute statircenewal GLP: No Year completed: 1999 Species: Pimephalespromelas Supplier:In-housecultureO.riginablroodstockfrom AquaticBiosystems, Inc.,FortCollinsC,O. Analyticalmonitoring: Temperature,pH, conductivitayn,d dissolved oxygen. Exposure period: 96-hours Statisticamlethods: The LC50 valueswere calculatedby Trimmed Spearman-Karber method. The NOEC valueswere calculatebdy Fishees Exact testusingToxstatstatisticsaolftware,Version3.5. Test fishage: Juveniles2,6 to 28 days old Length and weight: Average length 17 mm Average weight 0.037g (wet) Loading: 0.12g/L Pretreatment:None Testconditions: Dilutiownater: DechlorinateCdityofDuluth,MN tapwater,post- carbon filterT.he waterwas aeratedfor24 hourspriortothetest. Dilutionwater chemistry: Hardness: 42 mg/L as CaC03 Alkalinity: 54 mg/L as CaC03 pH: 8.03 LightingC:ool-whiftleuorescelnitghtAs.dailpyhotoperioofd16 hourslight and 8 hours darkwas maintainedthroughoutthetestingperiod. Stock and testsolutionpreparation:The testmaterialwas added directly tothe dilutiownater on a volume/volume basis. Concentrations dosing rate:Renewal at48 hours. Stabilitoyf the testchemical solutions: Not noted. Exposure vessels: 4 L wide mouth glassjarscontaining3 L testsolutions. Number of replicates:2 Number offishper replicate:10. Number of concentrations:fiveplusa blankcontrol Water chemistry during the study: pH range (0-96hours): 7.80- 8.03(controelxposure) 7.33- 7.89(720mg/L exposure)* Temperature range (0-96hours): 24.8- 25.6'C Conductivityrange (0-96hours): 125 - 132 pmhos/cm (controelxposure) 143 - 159 pmhos/cm (720mg/L exposure)* Dissolved oxygen range (0-96hours): 7.4- 8.3mg/L (controelxposure) 5.2- 8.4mg/L (720mg/L exposure)* 720 mg/L (intermediatceoncentratione)xposure valuesused because the highesttwo concentrationrsesultedintotalmortalitbyy 24 hours. RESULTS Nominal concentrations: Blankcontrol2,59,432, 720, 1200, and 2000 mg/L Element values: 96-hourLC50= 839 (766 - 920) mg/L 96-hourNOEC = 720 mg/L Element valuesbased on nominalconcentrations Remarks: Testingwas conductedon the mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportionalone. CONCLUSIONS The testsample 96-hourLC50 forfatheadminnow was determinedto be 839 mg/L witha 95% confidenceintervaolf766 to920 mg/L. The 96-hourNo Observed EffectConcentration(NOEC) is720 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:ilmischranking= 2. The studymeets thecriterifaorquality testing.However, sample pudtywas notproperlycharacterizeadnd itlacks analyticaclonfirmatioonftheamount offluorochemicaplroportioninthe solution. REFERENCES Thistestwas conducted by AScl CorporationD,uluthEnvironmentalTesting DivisionD,uluth,MN atthe requestofthe 3M Company, St.Paul,MN, Lab Request number Wl 127, 1999. OTHER Last changed: 6/28/00 AScl Corporation EnvironmentaTlestingDivision Submittedto3M - 2/99 AScl StudyID# 5010-069 STUDY ACUTE TOXICITY OF FC-203CF TO FATHEAD MINNOW (Pimephalespromelas) DATASTANDARD ASTM E 729 - 88a STUDY COMPLETED February22, 1999 TESTING FACILITY AScl Corporation EnvironmentalTestingDivision 4444 AirparkBoulevard Duluth,MN 55811 Tel.No. (218)722-4040 Fax No. (218)722-2592 STUDY IDENTIFICATION NUMBERS ASCI Study ID# 5010-068 3M Company lab Request#W 112'7 Sponsor: 3M Sponsor Study ID# W1127 (FC-203CF) AScl Corporation Environmental TestingDivision Submitted to 3M - 2/99 AScl Study ID# 5010-068 1.0 GENERAL INFORMATION StudyTitle Data Standard Sponsor Sponsor'sRepresentative Sponsor'sLab Request# TestingFacility PrincipalInvestigator ProjectDirector TestingFacilitDyirector DefinitivTeestDates Data Validationand Report Review Acute Toxicityof FC-203CF to Fathead Minnow (Pimephalespromelas) ASTM Designation:E 729-88a. 3M EnvironmentalTechnology and Safety Services,Building2-3E-09,935 Bush Avenue, St.Paul,MN 55133; Tel No. (651)778-7452. Susan Beach, 3M EnvironmentalTechnology and SafetyServices,Building2-3E-09,935 Bush Avenue, St.Paul,MN 55133; Tel No. (651)778-7452. W1127. ASCI Corporation EnvironmentalTestingDivision,4444 Airpark Boulevard,Duluth,MN 55811; Tel.No. (218) 722-4040. Joe Dierkes Joe Amato Donald Mount February 9-13, 1999. Alan Mozol Report Signature: Retentionof Raw Data and FinalReport Locationof Raw Data and FinalReport Two yearsfrom thedatestudyiscompleted. AScl CorporationTestingDivision,4444 AirparkBoulevard,Duluth,MN 55811; Tel. No. (218)722-4040. Sponsor: 3M Sponsor Study ID# W1127 (FC-203CF) 2 ASel Corporation EnvironmentalTestingDivision Submittedto 3M - 2/99 ASel Study ID# 5010-068 2.0 OBJECTIVE(S) To determinethe96-hour 10% and 50% lethalconcentration(sLC,o and LC50), and the96-hour no observableeffectconcentratio(nNOEC) of thetestsubstanceF,C-203CF, forfatheadminnow (Pimephalespromelas)under static-renewtaelstconditions. This stud was conductedaccordingtoASTM Designation:E 729-89a,StandardGuide for ConductingAcute ToxicityTestswithFishes,Macroinvertebratesa,nd Amphibians, 1995. 3.0 TEST METHODS Test Substance Test Organisms DilutionWater Properties:FC-203CF, amber solution,specificgravity= 1.0(water 1),stablea,nd misciblewith water. Testconcentrations0: (dilutiownatercontrol)259, 432, 720, 1200,and 2000 mg/L. Test solutionpreparedby volume additionof testsubstanceto dilutiownaterfollowedby stirring. Source: Healthy,juvenilefatheadminnow (Pimephalespromelas) were obtainedfrom ASCI in-housecultures.Originalbrood stockfortheculture was obtainedfrom AquaticBiosystems.Inc.,Fort Collins,Colorado. HoldinglacclimationT:he batchof fishacclimatedforthisstudywas hatchedon January12, 13,and 14, 1999. The fishwere acclimatedto test conditionfso.r 14 days. For the 14 days precedingtestinitiatiomno,rtality was 0%, and thefishwere not fed for26 hoursbeforetestinitiationA.ge of theorganismsattestinitiatiwoans 26 to 28 days. The fishmean length was 1.7 +-0.2 cm, and mean wet weightwas 37 12 mg. Loading rate was calculatetdo be 0.12 g of fishper L testsolution. Use intest:Used 10 organisms/replicaatned 2 replicates/test concentration. Source: Dilutionwater was carbon treatedCityof Duluth,MN, tapwater. The most recentchemicalanalysisisinRaw Data Package. The same batchof dilutionwaterwas used fortheentireacclimationperiodand the toxicittyest.The batchof dilutiownater was aeratedcontinuousldyuring storage. Characteristics:At testtime, the dilutionwater had a totalhardness42 mg/L, an alkalinitoyf 54 mg/L (bothas CaC03) and a pH of 8.03. Sponsor: 3M Sponsor Study ID# W1127 (FC-203CF) 3 AScl Corporation EnvironmentalTestingDivision Submittedto 3M - 2/99 AScl Study ID# 5010-068 Exposure Chambers Incubation Observations Endpoint Calculations 7@pe: 4-L wide mouth clearglassjar. Use in test:3 L of dilutionwater or testsolution/chamber.During the test,kept covered,exceptwhen experimentalobservationsor solution renewalswere made. Test solutionwsere renewed atthe 48-hourtest intervala,nd aerationwas not suppliedduringtheexposure. Duration: 96 hours. Dailyphotoperiod:16 h lightand 8 h dark,maintainedusingcool-white fluorescenltamps. Temperature: -25.0* 1'C. Biological:Daily- Test organisms with abnormal behaviorand signsof stressa,nd dead testorganisms. Water chemistry: (1)At testinitiatioand solutionrenewal-totalhardness and alkalinit(y2,)daily-temperatureD,O, conductivitayn,d pH. All determinationwsere made accordingtoAPHA methods (1998). LCIO: US EPA ICp Analysis. LC50: Spearman-Karber Method. NOEC-. Fisher'sExact T@q@oxst@at Version3.5). 3.0 RESULTS TestSubstanceToxicityEndpointsand (95% ConfidenceIntervals)P.ertinentdataare in Table 1. 24-h LC50: 906 mg/L (95% CI 862-952 mg/L). 48-h LC50: 839 mg/L (95% Cl 766-920 mg/L). 72-hLC50: 839 mg/L (95% CI 766-920 mg/L). 96-hLC50: 839 mg/L (95% CI 766-920 mg/L). 96-hLCIO: 576 mg/L (95% CI Not Reliable). 96-hNOEC: 720 mg/L. Sponsor: 3M SponsorStudy ID# W1127 (FC-203CF) 4 AScl Corporation EnvironmentalTestingDivision Submittedto 3M - 2/99 AScl Study ID# 5010-068 Ranges of Water Chemistry Parameters Hardness (mgll as CaCO3): 42-44. Alkalinit(yasCaCO3 mgll): 46-52. Temperature ('C): 24.8-25.6. DO (mglL): 5.2-8.4. Maintainedvia solution renewalatthe48-hour testinterval. pH: 7.33-8.03. -1Specificconductivit(y,umhoslan):125-177. 4.0 TEST QA/QC QA/QC Criteria Mortalityin controlexposuremust not be more than 10%. The lowestDO concentratioanttest terminationmust be atleast60% of theair saturatiovnalueat testtemperature. Testdurationmust be 96 h. ReferenceSubstanceToxicityTest. Data 0 % mortality. The lowestDO concentrationmeasured was 63% of theairsaturatiovnalueat25*C. The testdurationwas 96 h. Referencetoxicittyestingdataisincludedin the Raw Data Package. 5.0 REFERENCES American PublicHealth Association(APHA). 1998. StandardMethods fortheExamination of Water and Wastewater.APHA, Washington, D.C. ASTM. 1995.Section11.Volume 11.05.Designation:E 729-88a.StandardGuide for ConductingAcute ToxicityTestswithFishes,Macroinvertebratesa,nd Amphibians. Sponsor: 3M SponsorStudy ID# W1127 (FC-203CF) 5 AScl Corporation Environmental Testing Division Submitted to 3M - 2/99 AScl Study ID# 5010-068 TABLE 1. ACUTE TOXICITY OF FC-203CF TO FATHEAD MINNOW (P.promelas) .............................................. $ ............................ ........................ . ........ h. .............. 0 (control) 0(0) 259 0(0) 432 0(0) 720 1(5) 1200 20(100) 2000 20(100) ... .......... 0(0) 0(0) 0(0) 4(20) 20(100) 20(100) Siiii"inidi:.i.ieiricen::0 ::72::h 0(0) 0(0) 0(0) 4(20) 20(100) 20(100) 96 h 0(0) 0(0) 0(0) 4(20) 20(100) 20(100) Percentagesare in parenthesis. LETHAL AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS 24-h LCSO 48-h LC50 72-hLC50 96-h LCSO 96-h LCIO 96-h NOEC Data.(mg/L)a 906 839 839 839 576 720 .95@%Confidencelhterval 862-952 766-920 766-920 766-920 Not reliable Not applicable a Values based on nominal testconcentrations. Sponsor: 3M Sponsor Study ID# W1127 (FC-203CF) 6