Document pegmQ50D1BBJGK9RYQp2ZJeKD
BIOACCUMULATION (ANALYTICAL ONLY)
Err--7
TEST SUBSTANCE
Identity: PFeCr-f9l5u.oro(o1c-tOacnteasnuelsfuolnfaotnei;c amcaiyd,a1l,s1o,2b,e2.r3e,f3e.r4r.e4d,5t,o5,a6s,6P,7F,O7,S8,o8r,8heptadecafluoro-, potassium salt, CAS # 2795.39-3)
Remarks: Twhaes treesctosrudbesdtoanncteheispauriwthyi.te powder. No information
METHOD
Method: bDieovaicsceudmublya3tMe fClouomrpoachneymitcoadlestferrommin3eM'ifsblDueecgialtlurs,unAfLishplant effluent (See Remarks)
GLP (YIN): No Year completed: 1978
Remarks: The following method was devised and used:
1. 2.
Two One
lots of 30 fish each ot was exposed to
were used. Decatur plant
effluent
from
Oct.
5
to
Oct.
25. Oct.
The 3to
other lot Oct. 25.
was
exposed
to
Tennessee
River
water
only
from
3. tThweelrveemafiinsihnfgroemigthhteeeenfffliusehntweexrepossaecrdifloitcewdeirneDceoclalteucrt.edTdheeadriavenrd
water exposed were sacrificed
fish survived in Decatur.
through
the
exposure
period
and
all
4. aEfnfdlusehnitp-peexdpoisneddryanicdertiovetrhwea3tMer-EenxvpiorosnemdefnitsahlweLarbeoralaltporreysienrSvte.d
Paul, MN for analysis.
5. 6.
Fish Two
were homogenized using a 2g. portions were weighed
Tekmar Model and extracted
SDT using
tissuemizer. ethylacetate
7. oSnamopnleesanwdertoelauneanelyoznedthbeyoGthCe,r.TLC, and GC/MS.
RESULTS
Analytical Observations:
The effluent-exposed fish fish weighed 33.0 grams.
weighed
25.7
grams.
The
river water-exposed
0273
TLC
`was
qualitatively indicated
not quantified.
the
presence
of
perfluorooctanesulfonate,
but
No fluorochemicals were detected in the river water-exposed fish.
mRaenmuafrakcst:uresSftluudoryowchaesmiccoanlsd.ucWtaetdebreucsaeudsei3n Mm'asnuDfeaccattuurrinpglainstlater
treated River.
and
the
treated
effluent
discharges
eventually
into
the
Tennessee
DATA QUALITY
fRleluioarboiclhietmyi:caKlisimwiasschnroatnckhianrga3c.terEiffzleudenatncdotnhceenstpreactiifiocnsporfotsoucbojlefcotr aennoxatplokyssnuiorswoenfofitfhttehheeTrefeinswnhaewssassaenenyoRtoipfvpoeourrntwdu.antieTtryheufrosreeddweapinsurtaahltesiosotnnudooyf.itnhfAeodrdfmiitashitoipnoraniloloryn,foitis fsuacnrcitfiiocne.ofPtrheesfeinschecoonfctehnetrtaetstiosnusbshtoamnocgeenciozuilndghwaivteh btheeenefaflnueonrtmal gtcohinevce1en2nttodraewtahideotnfhsie.shrNitnohteehsxeupeblfjafenlcatuteicnohtne-meisixcpaoatslteewdmapgstroeaudcpta.usalNtlooywadhicafcftuemrweuanltsaiatttehideoncinwaatuhsseeofifsh
or simply sorbed on the surface of the fish body.
CONCLUSIONS
No conclusions can be made about the uptake of perfiuorooctanesulfonate
from Decatur effluent for reasons given above.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
REFERENCES
Study conducted by 3M Company. O0 OTTHHER 00000000 Last changed: 5/4/00
cgezro
am" Formener iva
TECHNICAL REPORT SUMMARY
JO: TECHNICAL COMMUNICATIONS CENTER -- 201.20N (important Ifreport sprints anbotsiodfpeapesr,ssn tcwopies to TOC.)
.5/1/79
Fe Environmental Laboratory (EE Fate of Fluorochemicals
Analysis for Fluorochemicals
D. L. Bacon
G. A. Vraspir/Arthur Mendel 48838-18
send, B20 comma ooiiumortsion
Emonos.
| cunnent omeerive:
& PC) Req. No. 48765
in Bluegill Fish
Ga 0535 2970612600
014
| "ESRD
7641643930 7
Ova "Ee
Status Report
LEPOSnTiAeBSTCRoAmCTo:,(W30B0.350 worl Thi ovat normation Gbuedby ou ToricCommarestomsComer 5
3:RV..AP.oPtrhoakporpa-g2a3d6a-- 286-34 ARI.NL.!VBeolhtoenr--2211--22% J etn aon |
00280
: FC (Req. 4876S)/GAV/AM
-2-
5/1/79
OBJECTIVE
The purpose fish, after
of this exposure
fluorochemicals.
itnovesoutrigaDteicoanturwasplatnot
determine effluent,
if bluegill bioaccumulated
SUMMARY
E(xPEt)racctosntafirnoemdbl1u0egpiplml(ufgi/sgh) eoxfposFeUd3t9o23Deacnadtu7rppplmanotf
effluent FM 3422.
wTeLrCeinnodticadteteedcttehde. presence of FC-95, while FC-143 and FC-128
Eexttharnaoclt,s eftrhoymlbleutheegri,lletfhisohxyeetxhpyolsedacettoatrei,vertowluaetneer, (RaWn)d cxyolnetnaei.ned No fluorochemicals were detected.
Aexpceorpiymeinstsa.ttached of CRL report 7124 which detailed GC/MS
INTRODUCTION
Tbyhefuipsthakweereandrepcolretaerdanc(e1).of sThpeecicfuircrenrtadrieoploarbteleddetafillusorotchehemicals
) Daencaaltyusresplfaonrt felfufolruoecnhtemiacndalsininfisfhisehxpporseevdiouosnllyy etxopoTseendnestsoeoour
Rtoigvrearphwya,terand(2)g.as Tchhirno-mlaatyoegrracphhyr/ommaastsogrsappehcytro(sTcLCo)p,y g(aGsC/McSh)rowmear-e used.
DISCUSSION
OmuarnufDaeccattuurringplainst lmaatneurfatcrteuarteesd afnlduortohechetmriecaatlesd. efWflauteenrtusdeidschinarges etvheensteuabliloyassianytoextpheeriTmeennntess,seewerReiveerx.poseBlduetgoilPl.E.fishA,notchhoesrenlotforof fiisshh wweerree ethxepnoseadnaloynzleydtofortheflTueonrnoecshseemeicaRlisvebrywaatnerinv(2o)l,vedThe tinhrethee-roeuxtpeeripmreoncteadluresecatsioonu.tliBnreidefilny,FlofwisDhiawegrreamho1moagnednidzeetda,iled wahnidlesomseomeofofthethehohmoomgoengaetneatewaswasexterxatcrtaecdtewditwhittholeutehnyel (Raocuetteate1), R(oRuotuete3)2).forAGpCo/rMStioanndofTLCt.he lReastutletrsexatrreacgtivewnas inconthceentsurmamtaerdy section.
00281
-
-3-
FLOW DIAGRAM 1 ANALYSIS OF BLUEGILL FISH
30 Fish
((21)) W"eTiigshsuenize"
55
&Ree,3
b>
(1) Weigh 2 g. (2) E(xEtTrOAaCc)t (3) centrituge
) (4) LIasvoelrate ETOAC
r _- Route 3 --
1) Weigh 2 g. (2) Acidity (3) E(xTtorlaucente) (4) Centrifuge
(5) TIoslouleantee Layer (6) Methylate
Be aocr
FU 3923 Fu 3422
Lo $s, -- Concentrate
Ee Ll $
Se
)
FeC--91528
FC-143
J 00252
-. | FC (Req. 48765)/GAV/AM
-a-
5/1/79
3) EXPERIMENTAL
TtbWyiOvetlhlyeo,tsEnavonifdrotsnhhmiieprnptteyadlfiisnLhabdoreryacahti,ocrey,w.rawpeprTeehdereifncisehiavlweuedmrieonnukmNepoftvoielmf,breorzreen3s,puenc1-t07i8l, Oacnta.lyz25e)d w(eNiovg.hed3, 251.9778)g.. TTwheelveefflfuisehntw-eerxepocsoeldlelcotted(Ocdte.ad5antdo e(Oicgth.tee3ntofiOscht.wer25e) swaecirgihfeidced33.i0n Dgercaamtsura.nd wTehreerailvler-seaxcproisfeidcedlot at Decatur.
wTaossiamnpalliyfzye,dthien eexxapcetrliymentthealsoamnee mlaontneirs. disTchuessfeodl.lowTihneg ocotdheesr were used:
River Water Holding Tank Exposed Fish - ethylacetate
River Water Holding Tank Exposed Fish - etxotlruaecnte e(xRt.rWa.c-tEtAc)
PPllaanntt
EEfffflluueenntt
SS--11
EExxppoosseedd
Fish Fish
-
ethylac(eRt.sWt.e-Toelx.t)ract (P.E.-EtAc) toluene extract (P.E.-Tol.)
TTwhoe 2fisg.h wpeorretiohnosmowgeerneizewdeiguhseidnginatToeksmeapraraMtoedelpolSyDTethtyilsesnueemiczeenrt.rifuge
) tounbeesa.nd T2womlmlco'nse.ofHCpLesptliucside2 mglradoef eAtRhyglraacdeetattoeluweenereweardededaddteod
tVoorttehxe oGtehneire. mixEearc.h sTahmeplesawmapslesmiwxeerdeacpepnrtorxiimfautgeeldyfo1rmi1n5. mionn. aat
oe1fx0ft,r0a0ac0ntdrwppmal'sasc.eedxapTihnienegdleatsuhssyilnavgciaelttsah,eterfeaosnlpdleoctwtoiilnvugeelnGyeC. ecoxTnthdreiatciteostnhswy:elraeceItdnrastatrwe.n -
H1P0%5C71a3rbowwitghx N2i0 eonlec6t0r/o8n0 mceasphturCehrodmeotseoctrobr.W-AWC.olumInng.- P6o'rxt1/-8" S.S.
TFelmopw -- 24000cc/Cm.,in.DefA.rgoTnem/pMet-ha3n0e0(8C5.,/5)O.ven Temp - 150 C isothermal,
uAnfdteerr NGpC paunragleysifsoroftwoEtAdcimeenxtsriaocnta,l TaLCporatniaolynsewsa.s cTohnecenftorlaltoewding TspLoCttceodndiatlioonngssidweerestaunsedda.rdsThofe cFCo-n9c5e,ntrFaCt-1e4d3,EAacndeFxCt-r1a2c8tsownerE.e MiinnerEc5tk0A%csEitlaiOndcHa dinrgieelEdt.Apcl.atTehseT.nhe thTdehreiyepdlwapetlreaestewfseirrwesetrteduernvveeidlsuoap90leidzeaidnndb1yd0e%vsepElrtoOapHyeidng with 0.01% New Fuchsin in water (3).
Tehxeggitnoelduenuesinegxtrtahcet fwoalslomweintghylGaCtecdondwiittihondsi:azomIenstthra.ne HPand571t3henwith
NDi>C -300eleacndtro1n0%cBaepnttuorneede3t4ecotror8.0/90Colmuemsnh g-na1k2'rxom1P/A8." S.ISn,j. 2P0o%rt -
J
24000cc/C.min.DetA.rgotnem/pMet-ha30n0e (9C.5,/5)O.venTh-e11e0thylCacIestoattheermaanld, meFtlhoywla-ted toluene extracts were submitted to Central Research Analytical for
GfCo/rMSGC/aMnSalycsoinsdit(iRoenq.s. No. C-50687). See attached CR Report No. 7124
00283
:
FC (Req. 48768)/GAV/AM
-5-
5/1/79
Y
PREW/&EtAPcE-Teoxlt.racsthowsehdowendo daipfpfreorxeinmcaet.elyNo10FCu-g1/4g3 oofr FFMC3-412228. was detected by TLC of the PE EtAc, but a spot was noticed whose
Ry was that of reference FC-95.
REFERENCES (1) a) M, T. Elnabarawy, "Bioconcentration of FM 3422 in
RBelpuoergti,ll 5/S1u7n/f7i7s.h and In Channel Catfish," EE & PC
b) A. N. Welter, "Aquatic Fate of A Fluorochemical, FM 3422," EE & PC Report, 10/14/77.
(2) ODcetc.atu1r8, Ef1f9l78uenatndFiFesbh. To5,xic1i97t9y; TeM.stT.PrEotloncaoblasr,awyd.ated
(3) J. Sliwick and A. Macioszezyk, Micrachemical Journal,
23, 121 (1978).
Fel] Vivapnspf
Maclsftom
GAV/AM/cen
Attachment
.
J
00281
--
dD
CENTRAL ANALYTICAL LABORATORY
Report No 712. % ___.. Date.Merch 28, 1973
Subject: Decatur Fish Bloassy.
Requestor:G:_ Vraseir,A Mendel Dept. Name EELPC
Request No. 50687
Report:
* Dated. Nove16m,1b97e8 r
INTRODUCTION
pp No. 315016130404
TvJbeeaincbnanietesugssreeyPholiNanrntietv.esrrheecweFhiirivsicvhehedrw.effrlrooowEmstehxtyJphluoessteardceoeqututtaoessittdowerosdtihfferfoepmrleaannttfiawsnahdtbelrthoeas!spspeyyteantcsaekoetnornieavtentthe dSl7aaznogneSthheanGsC/aMnSdsyasntaelmy.sed.Toluens extrtaectesxtroafcttsheofFisthhewafrieemmweanreel oonrersr?oZe0nds, oisucss on
)
#2 wA2arsveTra4er2aaraaignnyadzleydPBzLoeuEEdsinu1Jg2saibnceegoltnehdcdyoilntRdiLioMa.tncset1aftoerancedxotlPruLaEmc.ntsA1)).be(lnoSawatmhapynlldeastsedaRpotieoelniusNneeenesexst6rriaecactnSs)) pacn.d
+
ions for corum s pela dCIonslturmumAe:nt: 12!VaSr.iSa.n 2207840DCg-as200ch+rom1a0t%ogbreanpthone 34 on Anakro
Irnejne:ctor1:10C165Isothermal for 11 minutes. then 110-1050 ee m0cP/Aain.
OCvoelnu:mn 5B:0 Injectors
-62!222055%.C5. acCW1200%MC/m1i5n%.on
Chromosorb
W
CDeatrerciteorr:Gas:VarHiaenliufmlaamte 2i5onciozfamtiino,n at 230C
MSIoondusert:creu:mnaelanlst"C:h8es1ma3imdc8pualPleorsnuntIwoeI2nrn1ie-zC4ahr9teu1imn8oincmiaanlssElescptercotnromoentiezration, sample P.c. 12
MHMuealslstiiupmRlaiSnegerep::aratB1oe8rn:d-ix6S1Ci7hnagnnleneeslttaramn gIeonization with MH ger
00285
F000 Report Mo. 7124
x
avy care
d rests
SeaxmtprlaecstiAn.gW.and1 meatnhdylP.atiEn.g:FIetshhaonwoeld,byethGyCl/HSethoenrl,y etthheoxsyolevtehnytls aucseedtatIen toluene and xylenes.
oIbttwaaisn nseucfefsiscaireynttodataalrtoconIcnetnetrrparteet tthhee emtahsyslspaeccettraat.e extracts In order to
TG.hCe. TepenankessseneoneRiovferwheixcphoseadppefaisrhedetthoylconatcaeitnatefleuxortirnaec.t cTohnetacionmepdonneunmtesrous aphptpheaalratteo, coanrdreshpiognhdmotloecruelfaerrenacleksanoesf 2f-rmoemthayb|o-u3t-oCcytsantoonsa,t alceeatsitcCayc.id, a Twhheichplaanptpeaerffltuoecntorerxepsopsoendd ftioshreeftehryalnceascetoafte2-emxettrhayclt-3-coocnttaanionneed,coamcpeotuincds a5c8i2d,n/eF,lunoirtorcohgeennicaclosntwahionsienghiFglhueosrtocmeahsnsicaflrsagn(ewnhtosseseheinghevsetremasMs33fwraegmeanntds Seen were WB n/o and 584 m/e), FN 3923 (FOSE amide) and FH 3422 (FOSE alcohol).
SUMMARY
:
RThiivserfwiastherbiocaosnstyaisnheodwesduchthactompeoxutnrdasctsasfertohmantohle, feitshhylexeptohseerd, teothtohxeyTeetnhnyelssee
)
apcleatnattee,ffltuoelnutenecoanntdainxeydlenFeO.SE Tamhiedee,xtraalccothsolf,roomthtehre frieslhateexdpofslevdorotcohotnhiecaDlesc,atur
2-nethyl-3-octanona, and acetic acid.
; x Seley
%. W. Schrospfer rch
00236