Document pegmEQDzeaM49y0R1EvkDNoMd

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber isnotnoted. The testsample is FC-603EF. Currentinformationindicatesitisa mixtureof 1.30% PFOS, 10% diethyleneglycolbutylether,54.15% water,22.5% ethyleneglycol,2.6% propanesultonefoamer, 5% Alkylethersulfate0,.1% N-(3Chloroallyl)hexaminiucmhloride0,.9% Xanthan gum, 0.9% starch,1.0% triethanolaminea,nd 0.05% tolytlriazole. The followingsummary applies a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemkal component of the testsample. METHOD Method: OECD Guideline209 Test type: Acute static GLP: No Year Completed: 1996 Analyticalmonitoring: Dissolvedoxygen,pH, and temperature. Statisticamlethods: Resultswere determinedby calculatioonfthe % inhibitioinnrespiratiornate. Test organism source: Activatedsludgemixed liquorcollectedfrom the Metro Waste Water Treatment Plant,St.Paul,MN. Test condition: Dilutionwater: MilliporMeilli_QTwMater Synthetic sewage: per OECD method Reference and testsolutionpreparation: A stocksolutionofthe referencesubstance,3,5-dichlorophenowla,s prepared by dissolving 500 mg in 1OmL 1N NAOH, dilutedto 30mL with MilliporMeilli_QTM water,thenbroughttothepointofincipienptrecipitatiwointh1N H2SO4 and dilutedto 1L withMilli-Qrwmater. The pH ofthe referencesolution was measured tobe 7.2. Test substance was added directltyothe testvessels. Test vessels: Not given. Number of concentrations: threeplus1 referencesubstance and blank control Temperature: 19.4- 20.6 "C Total suspended solidsand pH on day of testing:2.96 g/L (TSS in final1:.18 g/L),initipaHl 7.3and finalpH 7.6. ElementBasis:Respiratiionnhibitaisodneterminebdy oxygen consumption. Remarks: Values correctedto200C forcalculations. RESULTS Nominal concentrations: Blank control,10,100,and 1000 mg/L. The blank controland 1000 mg/L exposure concentrationwere evaluatedinduplicate. Reference substance at 10 mg/L. Elementvalues: 30-minuteEC50=>1000mg/L 3-hourEC50 = >1000 mg/L Element valuesbased on nominal concentrations. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance 3-hour ECw foractivatedsludge respiratioinnhibitiownas determined tobe >1000 mg/L, the highestconcentrationtested. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking= 2.This studymeets the criterifaorquality testing.However, sample puritywas notpropedy characterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportionin solution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number P2581, 1996. OTHER Last changed: 6/26/00 ENVIRCNMENTAL LAE30RATC;RY AC-.IVAT-R-OSLUCGE MIX-CO UC.UCR Care 10-2'3Analyst Page N of 'g Type Test c@cco Ac--TivRTc-0 -@D o C--E3 PZ,5? 12R -T ic) r,)-TE'@@,i ?-C)c@ ACTIVATE[) SLUDGE MIXED LIQUog Cate Obtainedlo-23- 9(c Caie Used Mixed Uquor Suspended Solids(MLSS) g[L Adjustedto MLSS in finaltestsolution 9[L InidalpH (Isttestsoln.prepared) FinalpH 7-G Comments g/L Vast soin.prepared) TEST SUBSTANCE Sample' Description InitW pH of:highestconc. tested(iCCr,!Z!n -7:ZjComm ents'*,.,,k,,.-, Adjusted to PH, REFR=RENC,E SIJBSTANCE Sa.m4pi'e' D 'e's"ci@''pr'it"on' C) C@i9 lnidalp.H@ "6f'*stocksoiu-t,ion 500: mg/L), Cbmments@ 7.@ 2- to pH EXPO-9 RE CONDMC) N -7 T.7 st ur tidn Tes em ratureH.,.H e- c LR ENVIRONMENTAL LABORATORY CECD ACTIVATED SLUDGE RESPIRATION INHIBITCN TEST r4@@og TEST SOLUTIONS PRE:)AQATION Test Substance: P-c- Sample Description: Analyst: Date:@ Test Conc. nq/L rng added per 500 mL mL MG(l) water per 500 mL mL syn. sewige feed per 500-ML mL microbial inoc-.,Iuprenr 5 00__mL -2 '4 'TotW Volume ML C)D t its, ENVIRONMENTAL LABORATORY pa OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 RAW DATA Test Substance: Ft-(n03F-F - Source of Activated Sludge Mixed Liquor:(Y)4j,r,DLDck'b@'-cx-)C-k Date Received: c@ Date Used: Analyst: -2-3 -c@G 30-MINUTE EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS, mgl- Test Substance TIME-MINUTES Temp Concentratio(n.31L) 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 OC BK CONTRO I too I-CE)D 57.'9 C,7.9 L@ 9.1 4.!g 4.,:@ - - .2-:z 1-11 1.5 1-3 o. c@ o.-2 -1--11- LI-4 4.1 ,5.9 3-5 5 3 .4 .3.1 9.-6 o.-3 C)II 0.0 19-F 20.(, ;2o.L4 E@K CO c5@l @q@ 3,r DISSOLVED OXYG EN CONCENTRATIONS, ...-..-;@,ur-Tesl 66666tratiori,(rvlm0l.@5') -1'0.' 1.5 2.0 TIME" MINUTES' 2.5: 3.0 @3.5 410.11 4.5 1 5'.0 5'5, 'OC CX)t4TFIMI -7.4 -7'b -7.o g (o- S4 (t (..3 5 5-2- -6@ iV2--. 0.4 - 77, I_ IV n it Fi Final ... ENVIRoNmENTAL LABORATORY CALCULATION OF RESULTS Lr OECD ACTIVATED SLUDGE RESPIRATION INHION'@T.EST .209i. Test Substance*.FC-603EFAnalyst.Trudy Stiefenhoefer 30-Minuto Ex)osure lmg/L 0.5 1.0 1.5 2.0 7-- BK1 6.3 6.1 5.9 5.7 10 6.5 6.1 5.9 5.6 -TO-0 6.5 6.1 5.9 5.6 -TO0-0 2.9 2.5 2.2 1.9 1000 .0 0.5 Dissolvd O)XNVan Concentrations,m q/-L :7s-F-3.-O-F3.5 4.0 4.5 5.0 5.5 -1@l.'Tomp, -c, R: (1) 4'Ra'(2 ILL A@k AgggL- @5@ft a"" 5.5 5.2 5.0 4.7' 4.4 4.2 4.0,4k20-.6 0.4836@L 0.463 5.3 5.0 4.7 4.4 -4.1- 3.9 3@. ;T2o.6@, 6.5806 0.556 5.3 5.0 4.7 -_4.4 4.1 3.9 3.5 19.8 0.58061 0.588 1.5 1.3 0.9 0.6 @@0,3 0.1;,-0.0 Qz2@u0.o6u@ .0.6167,@@0@.591 2 0.4,4 AX*O-l 1BK2 7.1- 6.8 6.-1 b.4 % diffIn control(s4):,4..9 3-HOU fm-o/L @@ BK1 -10 - 100 1000 Exposure 0.5 1.0 f@@ 7.4 7.4 7.6 5.0 2.5 .;@%Wl 7.3 7.1 7.3 4.7 2.1 1.5 2L 7.0 6.7 7.0 4.4 1.9 2.0 @@ 6.8 6.6 6.8 4.1 1.6 % diffincontrol(s4): 3.7 6.0 1 5.9 5.5-,5T-@.3-F 5-1 ECSO (95%Cl) 7@.45-09 0.441 Ra Cm,(5 .,DissolvedOxycienCon@6ntrations'ma 3.0 3.5 4.0 L@4.5 5.0 @ 5.5 Temp.-c R",(1) :Ra (2 @ @k2@ AELL Eiii 00*'** 6.6 6.4 6.1 5.9 , 5.71, 5.4 0.4715-! 0,468 6.3 6.0 5.7 5.4 ,5.2 5.0 4.6 4b"20.1ql; 0.5382 i 0,534 6.5 6.3 6.0 5.8 , 5.6 5,4 5.-l @@,19.4 -0.4776 0.497 3.8 3.6 3.3 3.0,@ i@2.7. 2.5 2.2,, Vr@2 0. 0 0.5515@ 0.551 1.2 0.6 @i',@'20.6 0 7400- -@.-7-09 Ult Ull 6.7 6.5 1 6.3 6.0: 5.13 1',,,5.6 r@5.'4&;@ -)02,0.1@4-;,@ 0.4545,*.0.'451 (95%Cl). "Pncm EC50 (1) R. Oxnen %Oaks rate(mg 021-min) (2)Ra- R ad@ood to2WC@- JR/O@EX) (3) % InNbl@on l(RaCm)-PASI(R&Cm)1100 (4) % ditof*nceInomrois: 118&Bkl.R&B@QVR&Cm)100(Two emrole must be whnn iS% O'feacn cinw.p (5) Mean ad@ntedR, controls[-R&Bkl+R&Bk2y2 a- 1.07'2 R&Cm. mean &Lluslo8d,oordrols Bk- War* cvlrot,S- "mpW Tobs- sampletomp x@ ioos-ztrt; LR ENVIRNOMENTAL LABOIRATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBRWN TEST# 209 TEST SOLLJTK>NS PREPARATION Test Substance: -3. Sample Description: Analyst: v- Date: Stock Solution Preparation: Of,0 e c@ \c,,@0ek) L-04 LiIc) 1.0 @,3 -t@S C--"Lj, v'h-t v C'. c. Test Cone. mall mL added r>er500 rnL mL MO(l) wa'ter""""M@L syn','. pgr 500 mL feed-22r5 0 ML microbial -2-co 7, TotalVolume. mL s C:)o LR ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 RAW DATA Test Substance: C-k \C-,(@op @,e-r\c,) Source of Activated Sludge Mixed Liquor: Wclt'jAe rvo@ Y-ne. lc:!r"iA-j+. ty@ Date Received: C - 2-3 -9(,o Date Used: Ci -23-'IG Analyst: 30-MINUTE EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS@ nxyl Te-s-t-s@-ubstance TIME-MINu,rES Temp Concentration 1-)0 .5 1.0 1.5 2.0 2.5 3.0 3.5 41.0' 4.5 5.0 5.5 OC BK CONTROL I C '2 IC) c) z4--+ t4 -2- 4-4(D ;t(D BK CONTROL If 3-HOUR. DISSOLVED, OXYGEN CONCENTRATIONS substan66",,f, , @N . TIME MIN ES: 0 5,.,.-l'.o I 5 '2.0",'2'5. 1 3.0 3.5,,-i-4. BK CONTROL I 4@@ 7,4 Is's MgL 4.5 -@5.0 , Temp 5 .5; OC;" 0' f p ac eCg37 1, z7!Ci,,' EMPONMENTAL LABORATORY C&CULATION OF FIESULTS OECD ACTIVATED SLUDGE RESPIRATION INHION'TEST-i'209,'. ualo, Test Substance: 3.5-Dichlorophenol Analyst:Trudy Stietenhoote 3 0-M lnuto Exposu Dissolv d Oxyqen Concentrations,-mq/ [m-@/L 0.5 1.0 1.5 2.0 2.5 3.@@3.@@ 4.0 4.5 1--,5.0 BK1 10 6.3 6.1 1 5.9 j 5.7 5.5 j 5.2 5.0 4.7 4.4 1 4.2 4.0 j 2 0.6 7.7 7.4 7.3 7.1 6.9 6.8 6.6 6.5 6.3 6.1 5.9 20.3 0.4836: 0.4639 1 0.3309 0.3241 fKF-32 7--76.4 % diffIn controls(4): 4.9 3-Hou Exposure mq/L 0.5 1.0 1.5 2.0 BK1 7.4 7.3 7.0 6.8 10_ 615 8.4 8.2 8.1 613 6.0 5.9 5.5 5.3 5.1 4.8 20.3 0.4509 0.4416 RaCm Dissof d Oxvqen Concentrations,m../L 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Tomp,-C R Ra (2) 6.6 6.4 6.1 5.9 5.7 5.4 5.1 20.1 0.4715 0.4682 8.0 7.9 7.B 7.7 7.6 7.5 7.4, ".,20.2 0.2109 0.2080 [3K 7.7 7.4 7.2 7.0 6.7 6.5 6.3 6.0 5.8 5.6, 5.4 .1@20.1 % diffin controls(4): 3.7 (1) R- Oxygen Loake rate(mg ozi-min) (2) Ra-Rad@MedIO20'C- JR/oEil (3) % InNbalon- ((R&Cm)-R&S/(R&Cm)1100 (4) % dikemnes In cordrob: (JR&Bkl.RaBiQURaCm)100 (Two oontrolsmust be withinis% of each othw.) (5) mean ad@Aled fl,controls-IR&Bkl*Ragk2y2 Bk- blank oontrolS,- sample x- Tobs-2(rC Bacm. me&n 64USted A, oDrArolS Tobs- sample lemp 0.45 RR aaCCm@n 5 L Chairi'CoufstodyRecord, 3M EnvironmentalLaboratory F" 351?6-PWO fgA7 c*ty Addrom rvb-.- C*y@ 5tw4.Zo Coft swnplw or Dato lte Sample Date Tim Pop* Tam 3M F&cMy Contact 3L'I "Ilob*r 8 7 @r! @3@m Load P, N 3M Number Containers of each Preservavteis 0 Matrix c; E],@Samples SolitSdeAt n 2 3 4 5 6 8 "io 12 Item oi,li+' RelinquishedB y/Affillation Time Date Shipped Via',: Received 6 y/Affillation ovw Amolvo" saff-p- LatiPag- OVklw I pp_ p RA R6@e