Document peOerejpxzvdaDQDnDbgJakgd
Wildlife International,
Ltd.
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PFOS: AN ACTIVATED SLUDGE, RESPIRATION INHIBITION TEST
ADDENDUM
WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454E- 101 3M LAB REQUEST NO. U2723
U.S. Environmental Protection Agency Series 850 - Ecological Effects Test Guidelines
OPPTS Number 850.1010 and
OECD Guideline 202
This study was reported using a test substance purity of 90.49%. The test substance characterization was revised on 9/7/00 and the purity was determined to be 86.9%. The Sponsor requested that the results be revised based on the most recent purity analyses. The results previously presented were simply corrected by the ratio of the two purifies or a factor of 0.9603. No attempt was made to recalculate anal)xical standard concentrations or measured concentrations from original raw data. As such, minor errors due to rounding may be present. Attached is the revised summary page from the final report and the most recent certificate of analysis.
Wildl fe International, Ltd.
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P oio Nu4m54Eb-10e1r
REVISED STUDY ABSTRACT BASED ON A PURITY OF 86.9%
The effect of the test substance on activated sludge microorganisms was assessed by the Activated Sludge Respiration Inhibition Test Method (OECD Guideline 209). The test contained a control, reference and treatment groups. The control group was used to determine the background respiration rate of the sludge and was not dosed with the test or reference substance. The reference group was dosed with 3,5-dichlorophenol, a known inhibitor of respiration, at concentrations of 3, 15 and 50 mg/L. The test substance was dosed at 0.87, 2.6, 8.7, 26, 87, 260 and 870 mg a.i./L. After an exposure period of 3 hours, the respiration rotes of the test solutions were measured using a dissolved oxygen meter. The respiration rates in the two controls were 39.8 and 41.1 nag OffL/hr, or a difference of 3.2%. The difference in control respiration rates was within the 15% difference limit established for the test. The validity of the test was further supported by the results from the 3,5-dichlorophenol reference group, which resulted in an EC50 of 9.0 mg/L. The EC50 was within the 5 to 30 mg/L range considered acceptable for the test.
Differences m 02 respiration rates between the control group and test substance treatments were observed at all test substance concentrations. Following is a summary of the results.
Treatment
Control 1
Control 2
3,5-dichlorophenol 3 mg/L
3,5-dichlorophenol 15 mg/L
.
3,5-diehlorophenol 50 mg/L
Perfluorooetane Sulfonic Acid, Potassium Salt (PFOS) Perfluorooetane Sulfonic Acid, Potassium Salt (PFOS)
Perfluorooctane Sulfonie Acid, Potassium Salt (PFOS) Perfluorooctane Sulfonic Acid, Potassium Salt (PFOS)
Perfluorooctane Sulfonic Acid, Potassium Salt (PFOS)
Perfluorooctane Sulfonic Acid, Potassium Salt (PFOS)
Perfluorooctane Sulfonie Acid, Potassium Salt (PFOS)
0.87 mg a.i./L 2.6 mg a.i./L
8.7 mg a.i./L 26 naga.i./L 87 mg a.i./L 260 mg a.i./L 870 mg a.i./L
RespirationRate mg O2/L/hour
39.6 41.1 31.3 14.6 5.1 38.9 35.1 33.5 37.9 32.7 28.1 24.7
Percent Inhibition
NA NA 22.9 63.8 87.4 3.6 13.0 17.0 6.1 19.0 30.4 38.8
Wildlife International, Ltd.
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CERTIFICATE OF ANALYSIS
ProioNumber454E-101
INTERIM CERTIFICA TE OFANAL YSIS
Revision 1(9/7/00)
Centre Analytical Laboratories COA Reference #: 023-018A 3M Product: PFOS, Lot 217 Reference #: SD-018
Purity: 86.9%
Test Name
S_ifieations
[
Rcsult
Purityt
.;:_;._:_.!.v,::::'_..__:;_'_z_: _::_:_:__::_.,-:,__::_._:_;:<,:.'!.,_ 86'9/0
Appearance
WhiteCQ,smtlinPe owder
Conforms
fi_ OCP/MS)
1. Ca_mn
2. Magnesium
3. Sodium
4. Pot_ium _
5. Nickd
6. [roll
_::........_:= ........................... '"_ "__'...........
,_:.:::_.,:'._-,,-,.,__._.:_:a:,_:;_::_::,_ : _:::::::!_::_: .'-!__:::_: _::_:,:.:_::::]
_:_._ii_!_`_ii_;_i_i_`_!_!_!!_ii_>]
,,_:_,::_......._ ::_::_,::_:,:_,:;,:::,_:,_,::::: ............ ........... '_,:,::.:::.::_::1
_.._:i_:_!i_i_i:_i_2_]i_!_?7ii_i!_;!!i_i_._.:_i_ii_i!;:;i_.
1
_ ....._ .... ,-,_,_;_..................- .....................,._
:::::: :::_ =_:.::::V_:_m*:_'''::: ._ ,:_':'-::: : _:_:::._,,::::::_,::::::
I. 0.005 wt./wt.%
2. 0.001wt./wt.%
3. 1.439 wt./wt.%
4. 6.849wt./wt.%
5. <0.001wtJwt.%
Total %Impurity(NMR) ::_:'.'_:r:_!_:_:,.'._,:_::_:_:_:_:i::_:-:_:_?::::__:.:,_::._=,_::_+v:,:_:::_:1:e.9_3_*Jwt.%
Total%Impurity
i_;_'!_,'!:i_::i,_::i!:i:!/_::i;!ii::__!ii::i:_:i!_iiii_::_ii:!'i_::ii_:_:!/i_;i:!_:i 8.41wt./wt.%
Total%Impurity
NoneD_.,ctcd
R=iaualSolvents(TO) PuritybyDSC InorganicAnions(IC)
2. FI_ 3. a_._
4. Nitrm_
_.i,r_-i=
"_:.:;:i:+:,:.,:..:.:_.,..!.:...:!.i.i.:.i.._.:'.i_.!.-:.i.,_..:.!.;!.!._..:...._..!._;.:.:_._.'.:..'_.:.:i.:i.'!.:.:;.:.!i.!i_:__:;'i:*_:i!':__:i._!..L.;:.i:.:z'.:_i:_:"ii:::LN_?oin;iei;D: etected _e_i::_Y;_:__"i_ _g_,_:ii_#.;_ _i _i:!,_! _i_ii::_ii!: Not Applicable_
-;._:_;'_::_ _!_:_":'_"_:_:
:_._';_:
_:_ _
_._
_]I_
..... _!_
_@_,_i_ __
_<_,_ _!_i_
__!_
_,_
_i__
_i_i_:
.::r;...... :.... :_:':,:'_ ..... _:_.-':_':_
_ o59_% 3. <o.o4o_.0/o
4. <o.oo9_Jwt.%
s.<0.0_0u6_.%
<0.007"wtJwt.%
"O_mic Acidss(IC)
_
_;-_:_.:_:_!_ _ _,_:_
4. NFPA
_
'EImmml Analys/#: I. Carbon
2. Hydrog_ 3. Nitrogm
4. Su]fizr
5. Ruoria
COA023,.015A
!_.i_i_Ji_;',!l;_
_i!_,_!_!:_i_
I. TheoretiVcaallu=e 17.8%
2. ThooreticaVl alae= 0%
3.
_etical
Value = 0%
4.
Thoorctical Value = 5.95%
5.
Tho_rficalValu=60%
4. 0.28wt./wt.%
I. 12.4w8tJwt.% 2. 0.244wtJwt.%
" 3. 1.74 wt./wt.% 4. 8.84 wt./wt.% 5. 54.1wt.lwt.%
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t
Wildlife International, Ltd.
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INTERIM CERTIFICATE OF ANAL YSIS
Centre Analytical Laboratories COA Reference #: 023-018A Date of Last Analysis: 08/31/00
Expiration Date: 08/31101 Storage Conditions: Frozen _<-10C Re-assessment Date: 08/31/01
IPurity= 100%- (sumof metalimpurities,1.45%+LC/MS impurities8, .41%+Inorganic Fluoride,0.59%+NMP.impurities,1.93%+organicacidimpurities0, .38%+POAA, 0.33%)
Total impurityfi'om all teats = 13.09% Purity = 10(P,_- 13.09%= 86.9%
2Potassium is expected in this salt form and is therefore not considerexlan impurity.
_Purity by DSC is generally not applicable to materials of low purity. No endothcrm was observed for this sample.
4Sulfurin the sample appears to be converted to S04 and hence detected using the inorganic anionmethod conditions. The anion result agrees well with the sulfur determinationin the elemental analysis,leading confidence to this interpretation. Based on the results, the SO4 is not considered an impurity.
_I'FA
HFBA NFPA PFPA
Trifluoroa_tic acid
Hcptafluorobutydcacid Nonofluoropentanoi acid Pentafluoropropanoaiccid
_"heoretiud value calcu]atiombased on the empiricalformula,CsFtTSOiK+(MW=538)
This work was oonducted under EPA Good Laboratory Practice Standards(40 CFR 160).
/
COA023-018A
Page2 of3
Wildlife International, Ltd.
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,roj um r
INTERIM
CERTIFICATE
OF ANAL YSIS
Centre Analytical Laboratories COA Reference #: 023-018A
LC/MS Purity Profile:
Impurity C4 C5 C6 C7
Total
wt./wt. % 1.22 1.33 4.72 1.14
8.41
Note: The C4 andC6 values were calculatedusing the C4 and C6 standard calibration curves, respectively. The C5 value was calculated using the average response factors fi'omthe C4 and C6 standard curves. Likewi_ the C7 value was calculated using the
average response factors fxom the C5 andC8 standard curves.
PreparedBy:
David S. Bell
Date
Sdem_e_, Centre Ana_ Reviewed By:
L_r_or_es
John Flaherty
Date
Laboratory Mamger, Centre Analytical Laboratories
COA023-0I$A
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Idli[e
International, Ltd.
ECOTOXICOLOGY & ANALYTICALTESTING SERVICES
December 1, 2000
Ms. Susan A. Beach 3M Corporation Environmental Laboratory 935 Bush Avenue St. Paul, Minnesota 55144
Dear Susan:
Enclosed please find the Addendum for the following study:
1. PFOS: AN ACTIVATED SLUDGE, RESPIRATION INHIBITION TEST
WILDLIFE INTERNATIONALL, TD. PROJECTNO.: 454E-101
If you have any questions, please let me know.
Sincerely,
l(.RD/cyw8/beach Enclosures
Edward C. Schaefer Manager, Biodegradation
8598 Commerce Drive Easton, Maryland 21601 -Tel.410-822-8600 - Fax 410-822-0632 E-mail ecotoxowildlifeintemational.com EuropeanO_ice:BergkampwegI 7231CLWamsveld-The Netherlands-Tel.31(135) 75- 573848. Fa_c31(0) 575- 574813 E-maila: leopold_worldonline_