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AR226-3028 DuPont m R 712-95 HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE STATIC, ACUTE 96-HOUR SCREENING TEST OF H-21209 TO FATHEAD MINNOWS, gimanhalas pgomal&s PURPOSE: This is a rangefinding study designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. GENERAL INFORMATION MR: HASKELL NUMBER MATERIAL TESTED SYNONYMS TEST C O D B l j m f SPONSOR:^ DuPont Specialty Chemicals NOTEBOOK TD- SUBMITTER'S NOTEBOOK NO. COMPOSITION: CONTAMINANTS! OTHER CODES! CAS REGISTRY! PURITY! TEST CONDITIONS Wran4nal concentrations tested! 0, 0.5, 1-0 50, 500, and 5000 mg/L. Dissolved Oxygen and pH: Nominal Concentration (mg/L) At test initiation 0 Hours Dissolved Oxygen pH (mg/L) At test completion, 96 Hours Dissolved Oxygen PH (mg/L) 0 0.5 1.0 50 500 5000 8.7 7.6 8.7 7.6 8.7 7.6 8.7 7.6 8.7 7.6 8.7 7.5 7.7 7.4 7.5 7.3 8.4a 8.5b 7.7 7.7 7.7 7.8 7.5a 7.5b a Reading taken at approximately three and one half hours after exposure, due to total mortality. b Reading taken at approximately one and one half hours after exposure, due to total mortality. ... Saniffzed- not contain TSCA CBf DuPont BLR 712-95 HASKELL LABORATORY YOU TOXICOLOGY AMD INDUSTRIAL MXDCINE STATIC, ACUTE 9S-HOUR SCREENING TEST OF H-21209 TO FATHEAD MINNOWS, Pimaohales promalaa. COMTXHUED RESULTS Based on visuel observations, all solutions were clear, except for the 500 and 5000 mg/L solutions which were cloudy, white in color. Mortalities were 0, 0, 0, 0, 100, ad 100%, respectively. The 96-hour LC50, based on nominal concentrations and mortality, was between 50 and 500 mg/L. All water quality parameters were within acceptable limits. CONCLUSIONS H-21209 exhibited slight toxicity in a 96-hour, unaerated, static acute test using fathead minnows, Pimeohales promelas. WORK BYs .u.. , 'Ji / J V '___ jJames W. Wetzel ,y vToxicology Associate ;c/ -''*-- - /---- ---Mo/Day/Year APPROVED BY: / ^ ^ / s ` s -- ' Michelle R. Brown, B.A. Study Direqtor _ Mo/Day/Year DISTRIBUTION : Company Sanitized. Does not contain TSCA CBi