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ACUTE TOXICITY TO FISH (FATHEAD MINNOW) I TEST e SUBSc TANCEiouesaNcE Identity: rPeefrefrlrueodrotoocatsanPeFsOulSfAoAny,laFmCi-d1o(2e8thoyrlaascetthaetem;ajmoarycaolmspoobneent of [F(Ch-e1p2t7ad,ecFaCf-l1u2or9o,ocFtCy-l)1s0u9l,foonrylFlC--,1p0o8t-aXs.si(uGmlyscailnte, ,CNA-Set#hy2l9-9N1- 51-7) Remarks: laboratory as The test E2566-2. sample is Current FC-109-X referred to information indicates by itis the test a mixture of 19- 223%%ettheasntoslu,bastnadn2ce-,6%61C%4-wCartfelru,or1oa2l%ky2l-cmaertbhooxxyylmaettehcyolmepthoouxnydpsr.opTaensotl, material was from 3M production lot 2. The following `mixtureof the summary is abbreviated because test substance in a water/organic the test solvent sample is solution. a Data may not accurately thetestsubstance. relate toxicityof the test sample with that of METHOD: Methods: OECD 203 Test type: Static acute GLP: Yes Year Completed: 1988 `Species: Pimephales promelas Analytical Statistical monitoring: Temperature, pH, DO analysis: LCso values were calculated using the program developed by Stephan et al. RESULTS Nominal Element concentrations: Bk control, values: 96-hour LCso = 57 10, 18, (36-93) 32, 56, mg/L 100, 180, 320 mg/L 96-hour NOEL = <10 mg/L All element values based on nominal concentrations. dReesmcarrikbesd: iTnetshteintgeswt assubcsotnadnucceterdemoanrktshefimeildx.tuTrheoefvtahleuetersetpsourtbesdtaanpcpleiaess dfoettehratmimniexttuhreeiamnpdacntootftthheetepsrtessuebnscteanocfet.heNoorgaatntiecmpstolwvaenstsmoardwehatot Ppoerrtfilounoorfotochteanteosxiucliftoyncyalnambiedocfoentthryilbaucteetdattoe.the 000273 cCoOwNeCoLUsSiIoOnNsS The FC-109-X 96-hour LCs for Pimephales promelas was determined to be 57 mg/L with a 95% confidence interval of 36-93 mg/L. The 96-hour no observed effect level was <10mg/L. PSauublm,itMtienrn:es3otMa,Co5m5p1a3n3y, Environmental Laboratory, P.O. Box 33331, St. bDAAtTaAQQuUaAtLIyTYY Reliability: Kiimisch ranking 2. Testing meets all criteria forquality testing, but lacks analytical confirmation of test substanceconcentrations. There is a lackofcharacterization of the test sample. R0 REEFFEE0 RREENNC0 CEESS 0000000000 CThoilsumsbtiuad,y MwiasssocuornidautcttheedraetqAuneasltytoifctalheB3ioMC-Cohmepmiasntryy.Laboratories, Inc. of R OTHER ee Last changed: 5/17/00 000234 gEhrea : ] fit srosson = AtEtnG: PKCe.E3n8vWioCrhoaomcnpemadennytEallnabLaabrsa.sy 11 " ButldSei.ngPa7u1l-,20-M0i5n,nes9o0t0aBus$h510A5venue E stu Time to FathAeacduteNinTnooxvici(tFyiseogfhrEl2e5n66p-r2oselan) 3 Data meqriRe OECD Gutdetine #20) aun JaBnieolo1.gtaBcowm11an stun compres ox January 12, 1988 -- PERFOMAING LABOMATORY ' AralycicAalquaBt7ii2oc0-0CThoeFxnainttcaoclAeBoyCgyLLaDabinoevriantioorines, Tnc, .E Columb?a.,0.MiBrosxou1r0i87 65205 ABC LABOWTORYPROJECT1D i 617 Page 1 of 63 1 000235 2 Submitted By: lm Analytical Blo-Chesistry Laberatortes, lzc. - Si i ro BESTCOPY AvaaBL fO i Sheu pus Susegd a epralaavyt-egs= Supervisor, Fish Acute! a Jillerst shedA siinlleat i 1 Colfandeme 12a en "FSTCOPY AVAILABLE 1 RESULTS + 4 vv v vine ss ennnnnnnnn aa 38617410 " 000237 ABC LASS #36617-3 of 63 i SRE BEST COPY AVAILABLE L cShetLent CSrarceT ertinAa ef SM ek sates L oe era 3 3 ctuaetTeonstepttaytotfi2e8662 tTo FarEben FIoren en 3 gfB omrey oree aip nfee gmraetirgtssn -- 1 SflicrpreRiof en pre) Dose-Fesponse Lire at sb = : 000238 : iE A { . [ee Ed : poster persoweL LthaeborTcahotenodruicsettsu,loyf Itndhcie.rescvttauosdryNefo.ofr JtIaMhneeCoHpm.rpoajnSeyocvtewaansf,oMrrB.lAoHlmooahalaiysstetidcaIElLlnsBbieoSr-uaCpvheyer.mvtissciTrnnyeg Following ASC personnel assisted vith various phases of the scudy. same Jane. Bovean tavea Wallen Chet bremnke ames Po Sutgere, BLD. Tee Bologtet 11 Balogtat Bologteat Technician T SCuhpreornviicsoIrn,vesFtiioghatAicuotnesl Intetats : bone < vs Ln 000239 2 ABC LABS 436617-5 of 63 . FISH ACUTE TORICITY conpExDIY Subjeces "DAcEuotzeelToaxsi)ct"eAyBCoSftud2y56N6u-m2betro F36a6c1h7e.ad Himow (Zisephales Sponsor: 3 Company Testing Facility: AAqnuaaltyitcicaTloxiBclool-oCghyestDsitvriyatoLnaboratories, Inc. CPo0l.usbBioax, 1M0i9a7sourt 65205 (314-t7a-g579) Locatien o: Original Raw Data and Final keport: S3e4.CoPamupla,nyMinnesota 55106 Test Matertal 23662 Test Concentzationss Control, 10, 18, 32, 56, 100, 180 and 320 sg/1 Dilution Sater: 47.24mgo/nly (hardness), 32 55/1 (alkaltniey), Test Dates: Initiation = January 8, 156 Texstnation = January 12, 1988 Length of Study: Yo-hours Results: 96-hour Ley = 57 sg/1 (957 C.1. = 36-93 nia) 96-hour NOEL = <10 ng/L 96-hour Slope of che. Dose-Response Line vas 16 Test Specten: Fathead Minnow (Pimephales promelas) or Soures of Organisms: ABC Laboratories tn-house culture Mean eights 0.061 5 (20.037 g) Yean Length: 1) en (2,2 wm) onoe240 ABC LASS #36617-6 of 63 3 Sebteces Sponsors F1sw cee TRICITY Comper: "acuce Toxistey of E2366-2 to Fachest Wissen (Pisephates Brozelss)," ABC Study Nuzber 38617. ERwin 5% Company Testing Facility: ASCPsoeoatmliyiatRetitacsaTilhoWahBntaiosote-euCprhtseafPs5ea5r3yn5iLaGdteireattroercfsessi,eyIsc. | commas Test averted ise Rese Goncentrusions: Control, 10, 18, 3, $5, 100, 150 and 320 g/t Dilution Suter: f2 atuf (hardness), 32 371 atiatinieg), Test Dates: nicatio-n damasey 8, 1560 Tecatnacion - Javoney 12, 1988 Lerneh of Seudys soohoues Resulens S6-hour 16, = 37 mp/d (955 G1. = 369) sar) ] Soehour OL = 10 ng Schour Slope of the Dose-Response Line vas 16 Test Species: Fachesd Wivsow (Bisspiates provetas) Sebecs of Organiama: AKC Laberacories dn-fouse culture Haan Kelghe: 0.00 5 (0.057 g) Haan Lange 17 5 (22.2 52) 00021 550 1458 3176 of 3 Bs i 8. Sh A H ; 1 f vr, ; L E > fi k promelTuhse) aVcausteassceosusseedtyuotnogf cEhe6s6e2chodtso ouftaltihveeadd bsyitmheu Co(rHmeiscpiehealeosn k EPBeiatvthosoumagesht'oeurtstortohfeToxcGieecaniptteyraantTduersevt,esredviivstishtehlivAaesdtsclocexeypgteaOn.rbglaeannildsemmsi.ptHs.' vKeartCeeorltcueqcsuessulriaetndyd F k SeliEtion Tecords indscaced the (10h vere fn food condition for E2366T-h3e 56,100, st10u,dy 180 1a6vn,aas733c,3o0n3d6uw,cgt/1eL0d0,saotl1u6c0thieonndfsol3hl2ao0dwisng8glLwp.heistiAencalsOuhcraofunascc,eentcfzhaietei.1c6n,s T3ho2ef, SiTcThiuhnGt oGfaouunrtisIcne chfeila18 `ianac/r1easseodluwciitohn tthoe acotnhciecnktera,tionnesclfsreeco3mpvaecrty hC6ao1dv5"eraiSn1sgo0coivaaepcrpeeatfrhieelda33oi0vnecrmreelca)hseionlg1u0cv3ii5to/hn1.tsheoAlfucetoeinrocne2nWthrBiaoltweieosnt,.he 5 oAtch1eitrghheeso7sl3uu.rtfiaaocnneds CSao1h1aociuSnottlruaotcbiisoeonrnsv,athiaTodensn.a ftishsuehr,f1a0cw0ei,ch(118180s.mdencadhnat32v0eiingecphr/teLaosfseodl0u.it0ni6o1npsro(ep1or0r.ee0i%ol)nouitsaneatnhde3 eCionncenSctraansdiaorndsnldencgotnhtroolf. 17 (2.3) mm, were exposed fo ach test l2e5a66G-Te5hresaicnreetsnouevldmt.ssriTovhfeed9c6bha-elhoouuf.rourelThodepaey2ofsseta4hc9ei-cdoasnfedashrT2e-sthpoooxunirsceLiit1y160svtavulodsyies16u.vseirneg Seetous Leg, compount 031 c.1.) s66-2 57 sai (6-93 mrt) E StornaotriaoAalnstoc,oeufltfdheesbcesresseoucflitnsasceertdtaadliaicttayt<,e0d saaugr/9fl6a.-chitonhuger,,lalsvooew-ssstheocefornvceeedwnwcierlfaifcbeircoitnvetc,eosntdceaedr.nk Ci1xe5cc:io5co6arb,ailcii1t0o0yn,,ve1r8ie0ocn`basndoenrv3e2td0he.dguibra]itnognt.etshteof9c6oc-nahcsoetunrtrchaeatxsipbooensrsu,.re epi`eOenresicodeinsichne cfhanend/c1oh0,re Control commber vas quiescent 43d on the boctos of the test chasber at bi 000242 pgm ae Laas 12661727 of 63 E i. f Dimonterion me } HpDiiuvaiwnsoiuTeorhnti.,sffosofrceacnSiWaelCyoebmiipcooasntmys,ayfbrioowm-acsdShaumspoieae nrrctyroyr3s.aALda1s0a0brsyatteecrhiSecoomsbAaqruTssbLteie1cs5,, T1`oex0sitceoolsHosdeg"ey Ttii for "EY 12" fachend simon (Eincabates sreseion 3 JBBpKiaeConeuulaainrFndyityan.ia4or.cyoT1lhr58ea8n"Sgtueascdbfeyisrnudtviea6rnsb0gi1cnsao.ennd(dutRychoteweadSsDunafccoceotanni)ttoorvraiottnneisdoniphFeoropokpmresaTcheefssoesrbtcyiesshReaoeudrsomsamoeasreas?hoef Tisabarasy, 4 Compony, on Janiney 7s 1568 ions a0 warren The procedures for ueacic biossany, as described fn stots of ArhcEeuteSeaTnoexsiecditVyotTiecsttes fwairthEFisRh, EMacrooirnvHeretrebraateseandriuaphibisans ((117) E [ERE PsFheiarstehopTce`hhrereiaeoCidantehhsdenendssr"dcobeaTmtnenrmanvo"eneduAlB(tCafUroCer.LuLabofrataLni4een2s-2hs0o7oufns1oeou)rctueiHesvneooeredrs.aegten AptMhYie otenesnset Selim k IN SdBEhreeaenncpcttiirvnyiagib.tseic:oeniPbseiytohfdBucesruihalnetngtuarpe.estuthsetteeacLhW.Snalilaqi(suia3eov.sgs sSper"eAeerdsitodsuewseie"ntrtyes,ebrmeaocg'soirstnmvsii.ovtsietd"h'Fooseoru]nsk ] Ge" sues" Dicing this perish ihe Fivh Facets 3 seas 3 SCCdiehunaeracianien'ySgonnmaaeeutrdpchwidhoTlinocr(hFhlSeSaehinemeea"fioaetoxdaapsneds.ric)inemgaeSsnaiwtioanlsbsiuSulindyeestiuelpnnsc4eii0snes-ur3e6ewtn,eshioegsdehisnseiocstehfioocabnnrreiesnnnsee : i Esh0iee1vTe0i'n3Cd7oen)ntnceehsadsaegntrdCoshu.speb`oeaSfraansEgtiwosheshosnaiiannatdrgd.tLShuelnTogesemsshupsresnaheinsoosaffstrie1o03sn.o0nC0ot0fn3cgShetevewcoFrotosrea"hmaoyiegdrs ; . ES RE Bach: IL Tess vacersal SgfiootorpdeieTChaenedpliusrl2ii3eot6rny6,-2qwusTiihaseam'"plsneacemeepv"laensUpureEreboctnetievirreeedcedediopneTaDhtweeceersdsooeebmsstoims2pv9ii,eidon1no9y8n7,eboes:'1tSn B :3 CCFCoeoreantttelecnCticoiaen.cdeesnitofCrnoaasrteiovsCnessorpemhwpeerr`eaavpbcuraatseteeicsruc.olnybytMhoe1'cirShaeseusensa!icneeieSrsreerisdopgBoruannestskbypoiereSissrspta.e.in8hTntSs Frit wpe rly 3 00D0OES NC LES v3e8i1-8 of 43 a AR BS oA : IL Test kater i gColmopsossTevhdeessosfeclastchiceconftfoailislhnoiwnibgnigo1s5csoaLmsipytoesrvssassofcfnoonfdtuchcetresedcmeor1traceiftiusveteaetdegdcaaltlpeoernr Ticer of detontzec vaters 48 5 Narco, 30 2 250,22 1,0 30 25 Hs50, iy 2a xa h2a3c1daeTaahssisCoafCrOe,4c0o-ma4sn8edsewaupnt/e1dtaaiw3caitCaealrCOBw.iasoafpcroe1r.za3alceCaotitTaeoiti.sviieishdeofa CoScot9re3as8l 3 Sdeiasssuorlevdedcooxnykgoeln fren Which this dv9ia.ltuetroiiopnlarwaaacntedecragtisva3so.f6.ptrheipTsahreSeidsiaulhiiaidcnCacrsheirvecrshaovruearscs Ceristics shown ta Table 1. a(n2d1".0c)Ta.hsee TechesemcpteersvatetsusrFeellasanvwdeerreheeladkcecpiwtiiztahftoneudtacfoovoadtthee:fortialc4uht-t5o6anc hvoa2tu3errsC pFraiocret.o testing. An acclimation record is included tn eh ~ TW. Biologseal Test Procedure Tfhoe'depAtreerlrmiainnnigenea-trihynedCiCeonnngtcetcneotsnrtacceoinfocnis9cF6ia-orhngoseuefsvoerrdeutrhaseteitGounEaitsnai3st0i,vceo1n0ds0utucdatyne,dd J i 3cS2eo0rnicee2sn3t/r1f.artoinoBnas1s0eodtfoonth3e2t0heGmegsrt/els,uclotowpsiocuhfndt,perneFlofiringsithnrgarpyeirn saecaotlnicineggn,ittraisteeivioecnn HE wC3ahradestblesurecaliiobcnytewdraatnfedororn ctaohsnestirdgoenltm.ennittTihWveiethfbilonashs3s0vaemyrie.suatAieldsveod'aftitenorciteshtieedittiseoesnst "3 1 3do4efadhtoeusItrnsdmiafvtoiredrumiaaollrs.talveiArtLey aCrneedrmtosvobeurdograeFnarilosne(stuvhbee-rieoeecrhhteale)r"vceshsatmeobcnercrse.aefvteaernryy B1 each 20-hour abservacion. Vo Botopsean bata Anatysts : ; % L(gEe.nerSracolanlcyrisaesaioccdacleavleialcnoyap)leydvaatssbyoofbSetacethpneheadcnonbecycenetxarlpa.ltoir(oi0nn0:gvea.cTohemifpsfoetcpetroitdsoietedan {i 4 ScMteeioscnltsilsocu.islnuecues"dicMcooautelhvedtehre,1R6,o"tElifabces'atnlaoadclei,samocovttrschteearlaamintoedevydiintgooacveae5vrv5eerr3eapdgeeTrecasearnsntadsiafccbholenefaipsrredooprevseeieer 5b CITED on Logg andlor fee 55 percent contidence Jintes : 000214 43C LABS 26617-5 of 63 E 1 BEST COPY AVAILABLE } E f i = 3 FRTooruolnEees enSovTteesrSbeSrohfacsaUlirSceteuhnrlriiahtvoieitndheic.oeensTmheohsreeteeeemmsoeedeaieevftefeehenroesrnhtJmcosaeercirnhaaleonradmerasioootnefoftiagenmanaaailtpyy5psceiihrSinne,dgg a j j ] rEFBeoecereeeentsc"kia1n63recSeoariechultauitteydna ohyarUeesEawsas"HSceeorvmastoreeneserionemdeoenmeeiaLbnistHeats, ef2o8r STEh5hSeecencescion ve. provi een trtteion snalyets | asars F(aPpeicTneheievsyi.noistiheecifestAvheeumSerhnuheodussehvoonseufEaotoifEe0sesniesedyvpeorrteeeesptmsaa,vilchafraeabsnohedenn5db7samEiig5mn7nh3eee,e 1FJ u2i3rn3en0 Cc11enkit1oYvn1epssuhienaA dSwersneeainbcteeassdelieoofnv. eoSe hvneemb5eat stebaargyppimreeotn2gtT 2Cm,eue6ek1S 00wA,ars1T18A6CDE aBoeperreaisionSigonla.cihsnothhuiecteioorcno3 2n4Sehhtahsceekauecrrietsohsne:eSa"hrsiplegesee.hCSemooopTrtioeeestes(hSSopaaennrnngge mSIheerrAnetcShieo2e1c3rh20e TA0C0.00 nhaec Sn30crSevHveds1feetgorweeiroen SolooaShte maeeriyn sete thems sTI1ho0ehreTaeobcsiTte,o"n, orfcaccaSsieonmgehe,eecthSoooun,scoonafcuetA anecniocine,nei-- venedesnt-- awckheh,St-- eernoetmaest-- ioosnirieoei-- thstSoFf To. 2% or V5 S330 lhe amityverebteryed ] ] TSR5Eo0losnaaiaretasiShsCeinaestenvThOiaercheaodrBaP1s0ei0%tfiCohohrakoedhriSSo0eesn5veeameteCsirtooenasiteioaendayieanuerettedeoeihomSaneebectnhcirpanehCgieLerhnEehseesead1ugH8rueit1eensgac4te32hrh2deee i iin To Ceting an acs csrLxIeoeTnnEtpSNasroeemhisienaamfledu3t.e8etsntwertTncoo0hebnic,euentirpaoethicnhoktnheiseev,endSsteioyvree,chasolaienemcsyeeovsrearcpeSSecspoaosneeeescdtniveeearcerrecsratMsqeiehuoeaqlnelusitietteryLeatndfage2ote2ccedda RR A a at Ee, % a C002 arc Lass 13061710 of 63 SPeiaocmeCiThchaeestPasoctptuuydlyatLviaaobsnosr.caotno(d6r)uiceotsne'dd Chfuoeslltoiiunsien]gAsetruherepaonrfctae.cveasLseiovsfe.vitheeuAeLGsoovoderiSLgaotbmuodtrspatEosiraoys CHaieenSchamsietptryovLaibdoratto.ories.Cospants wish a cops secetoed at Smpeteny 000246 ABC LABS #36617-11 of 63 bE Temperature Dissolved Oxygen" pH . Hardness (Caco;) Alkaltatey (caco;) Conductivity Total Organic Carbon Suspended Solids Aluataus. Boron BESTCOPY avaILABLE. [E 17 - 2 7.4 = 9.0 ppe 7.6 - 8.4% 206 ~ 275 ppe 3 224 = 336 ppd 500 - 650 izhos/ca 1.4 ppa (0.5 = 2.8 ppm)? 0 - 1.3 pps . <10 ppb p 2.25 ppa k 1 Mercury 0.43 ppb 3, 3 RRC TE *Afcer aeration PRecresents seasonal variation, difference from one month to Average of ell well vater values from January-Septesber, 1987 "Total monthly range of organic carbon ona? ABC LASS #36617-12 of 63 P B N : hn - 3 * Tae 2 Acute Toxtck(tBylzoefphaEl2e5s66p-r2osteolaFsa)theat Kionow : Leg tn atlltgramsilicer (ppm) Compound 24 hours 38 hours 96 hours ase' 220 1600 59 a0 esn k = 10 13h per concentration. E 50f.o0a6s1sa(y20a.s03c7)o5ndsuncdted17at(:22.27)Cem(.:1.0), sean Fish veight aad length, : 5164, could rot be calculated due to nsuffictent sortalicy data. 45% conftdence Lista. i aTghiet.96-hour no-observed eftece concencration could be estimated at <10 5 TLheeast96s-qhuoaurresslroepgeresosfiontheanadloysset-sr.esponse line vas 16 as calculated by ArLLevalues vere counded to two significant figures follovicg ASC 5.0.7. ) 164 calculated vatog: Dpsnontal Hetbot @oving average Method Opeonse vechod E 000218 ABC LABS #3661713 of 63 & = BESTCOPYAVAILABLE 1 T Torstar el es ti Ty To ] z Ferrin i tH ; EorEEoLana 1 EERENREE THT 5 ; EE -N BR Soe o . LiTsRATURE Cr7ED : (1) "ECB.omtSctetpeheano,n with Fish! CMMheaatcthrroosdtasnnv)fe.orrceTb1or9x7at5t.cetseyMeaTtcehdsotdssAsvpifhtorhidiAAaqcnuusat.teic|ToOExrmigvcaiinrtioysnmsseTnecs(atCls. APprroitle,cti1o9n75.Age6n1cyp,. Ecological Research Series EPA-50/>75-000, A (2) At3mheepriE.cxaanninPaucbiloinc oWfeaWlatther AasnsdocNtaasttieovna.ter.19801.3ch Setda.ndaardshiYnegtthoodns, Dfeo.r J (2) oBfrauRtens,eaJr.chL.Fisahn:d and Blucgills, R.RaAf.nbSocuhoTertocugte,r.Fat1h9e7a5d. Environsental Protection MiAmcnqouuiss,itiCohansraensd CCualtttuirieh Arency. Eetiogical fe Seazch Series FPA-660/3-75-011, Yay, 1975. 45 5. (4) S1t9e78p.han,A Cc.espE.u.terK. prA.ogrBausscfh,or Rc.alSceuiltaht,inJg. aBnurLkCe.and Cf.5.. SAanvdirreewn.e mmaennutsaelripPer,otAeucgtuisotn. 1A9g7e6.ncy, Duluth, Mismesoty pre-publicatien (5) lSFitsoezpalhradn,MEavyaCe.lruat1ai9no7dn7..J. AMSeLTt.MhoRdSaspseecfltotarnlkcaTle(eceuhdlnsia.ct).ailngPuaAnbqulaiLtceialgt,iTuom5v.ic35o45i.-e3gs.ASaTnIdn Philadelphia. (6) (2) (LU2i.1bSu.CfFaRc,oFroPyoadrcStaun5d8d)i.esD,rFuegdGeoroAaddlstaRLfeasgbtiorsratatetiroo,rny.Vol.Pra14c93t7,6i.ceNo. SSeopnucilaitsiiocnasl 20739906 6002s. (9) (PUr.GoSgC.rPam,sE;nvPiaGrroocondme1n6tL0aa)lb.oraPtFroeordtyeercatPliroancRteipActgaeetnecrSy,t.anVdoalr1.d96e3:.46,FinPSaoel.stiR2c3ui0te;e 53946-53569. k (6) RsUut.laSen.ce(s4E0nCvCoiFnrRto,rmoelPn;atracGloo7dP9r2o)Lt.aebcotriaFotenodreryAaglePsrcRayec.gtiiscte1e9r6S,5t.anVdoal.rTdosx;i4c8;FiSnuNoab.l- E 230:53922-53944. 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