Document pbvpXnE26JzpEe5qyK69MK6a

Younger Laboratories Ili&ckemUti. . . . . . AhoL^U ixOUTE 9 -- Box 281 ST. LOUIS 23. MISSOURI PHONE: TIlden 6-2540 2CBJSCT Toxicological Investigation Of ' '13-95.1 Monsanto Sample Number 17 iionaanto Project Niaaber Y-po-li Tebruaiy 22nd, 1958 Cfcservaticns were made for toxic symptoms and the viscera of these anials that succumbed was examined macroscopically. The data are found in Table II. C) Skin Irritation In Rabbits 03-95 was applied undiluted to the clipped, intact skin of three albino rabbits and removed after twenty-four hours with soap ;rd water. Observations were made over a period of several d^rs for inflammation. irritation was scored according to the method of -raize, Woodard and Calvery (Journal of Pham, and Ox?. Therapeutics, 7ol, 32, ho. U, Dec, 19hh), Txie results are given in Table III. DSW 586271 This confidential report is not to be used in any form of advertising without written permission STLCOPCB4091605  -t _ ?*" Koueanto Chemical 0 my, St, tmis* Missouri ' lounger laboratories Certificate of Analysis - Pago 2 (2/22/58) . T.58-J4, EXPERIMENTAL PROCEDURE - (Continued) D) Eye Irritation In Rabbits 0,1 Milliliter of sample was placed In the conjunctival sac of the right eye of three albino rabbits. The eyes were rinsed with wan isotonic saline solution after twentywfour hours. Observations were node over a period of several days for irritation. The data, scored according to Drains et al, are found in Table IP, E) Vapor Inhalation In Rats Four nature male rets were placed in a metal chrnber of approximately 25 liters capacity and exposed for six hours to a concentreted atmosphere of OS-95 vapors produced by aspirating the liquid at room temperature. The sample (250 grans) was poured into a three hole, round bottom flask and compreseed air passed over an orifice, the lower end of which was immersed in the liquid, A rubber tube led from the flask into the chamber. A glass implnger was placed in the line to break up droplets and allow, as nearly as practicable, only vapors to pass. The animals were cheeked for behavior and, sines there were no deaths, all were held fbr ten days observation. The data are shown in Table 7, SUHMAKT The Oral LCgg of *5-95 was found to be 10,500 milligrams per kilogram with lower and upper limits of 9,295 to 11,865 milligrams per kilogram. The Minimum lethal Dose range by Skin Absorption in rabbits was 625 to 1,250 milli grams per kilogram, 06-95 was classed as a moderate skin irritant, OB-95 was classed as a mild eye irritant. Ho deaths resulted when rats were exposed for six hours to a ecucentrated atmos phere of 03-95 vapors. TOUHQER LABORATORIES b ^ i'cnnai pub I Lad on 3, may be made without ment in St. Louis. Cut toxicity are to be refe uient in St. Loui3 for r 3 DSW 586272 STLCOPCB4091606 Tot Monsanto Chemical C any, St. Louis, Missouri. Younger Laboratories Certificate of Analysis - Page 3 (2/22/53) - 1-53-4 TABLE I THE ORAL LI: 5Q OF '06-95* FOR PATS jaal No, - Sex 1- Male 2- 'emale 3- Female L- Female 5- Kale 6- Female 7- Female 3- Male 9- Hale 10- Male 11- Female 12- Female 13- Female lii-- Female 15- Male 16- Hale 17- Female 18- Female 19- Female 20- Jfele Weight 3m , 270 195 210 190 230 205 195 215 230 245 135 190 210 225 240 220 215 200 190 195 rose Mg. / Kg, 9,000 9,`000 9,ooo 9,000 9,000 10,000 10,300 10,000 10,000 10,000 11,000 11,000 11,000 11,000 11,000 12,500 12,500 12,500 12,500 12,500 Fate Survived Survived Survived Survived Survived Died Survived Survived lied Survived Died Died Survived Lied Survived Died Died Died Survived Died The Oral LDao was placed at 10,500 milligrams per kilogram with lower and upper limits of 9*295 to 11,865 milligrams per kilogram. Survival time was overnight to three days. There was diarrhea, salivation, tremors, and coma. At autopsy the gastrointestinal tract was gaseous and inflamed, Thera was liver discoloration due in part to congestion. DSW 586273 STLCOPCB4091607 Tot Monsanto Chemical jany,. St. Louis, Missouri '. ' . Younger Laboratories Certificate of Analysis - Page U (2/22/58) - Y-53-U ? .A 1 L E II t:ie minimum uthal oss of os-95* by SKIN A3S0.PTION IN RABBITS Animal No. - Bex 1 Temale 2 - female 3 - Male h fills 3 - Male 6 - Female 7 - Male Weight XAf - 2.3 2.1 2.6 2.6 2.U 2.1 2.5 Vvse Mo-. / 35o 625 1,25o 2,500 5,000 7,500 10,000 Weight Change 5 Bays After F c s ing *? - 0.2 - 0.2 - 0.3 - 0.2 - 0.2 - o.U - o.U Fate Survived Fuivived lied - 11 . ays !led - 5 Oays lied - 5 ' ays lied - U fays tied - 3 lays DXSCOSSICH - The Minimum Lethal lose range was found to be 625 to 1,250 milligrams per kilogram. Survival time was three to eleven days. Appetite was soon affected resulting in weight losses. This was about the only toxic symptom for two days. Weakness and lethargy were noted thereafter. Violent convulsions developed in two of the animals several hours before death. At autopsy there were extensive hemorrhagic areas in the lungs and moderate kidney congestion. DSW 586274 STLCOPCB4091608 Toi Monsanto Chemical C jsny, . 3t. Lewis, Missouri. lounger Laboratories Certificate of Analysis - Page $ (2/22/58) - T-58-U Anin&l 'Aunbe r 1 2 3 Ave rage T, v xJ.X A.A AITS AFTEL APPLICf ION OF 0S - 95' Huneri cal Evaluation .-it The And Of 2 Honrs 2L Hours U3 Hours 72 Hours 120 Hours 2 `"'J 3 3 2 2 1 2 1 1 13 211 1-6 3,0 2,0 1.3 1.0 iiscuSsiaN - After two hours there was slight to well defined erythema with no edema for an average score of 1.6 out of a possible 6.0. Overnight the average score increased to 3.0 on the basis of more redness and slifdit edema. Tire application was removed in twenty-four hours but the irritation decreased rather slowly. Slight erythema was still present on all animals on the fifth fay. 13-93 was classed as a moderate skin irritant. DSW 586275 STLCOPCB4091609 To i Ifonsanto Chemical .pany, .. St, Louis t i-fissouri. ' lounger Laboratories Certificate of Analysis - page 6 (2/22/58) - T-58-U TABLE 17 EYE I;l.-1TATI0N IN BABBITS AFTER APPLICATION CF *0S-9 sal Number 1 2 3 Average Aiiraerical Evaluation At The End Of 1 dour 2b Hours L'3 Hours 72 Hours 120 Hours -1U*O_ 10 vj 6 k\ 4 2 0 0 0 12 6 h 2 0 oo. 11.3 7.3 4.0 1.3 ::TSCU3SI3f - There was mild immediate discomfort shown upon application* The average score after one hour was 11*3 cut of a possible 210.0, Irritation consisted of moderate erythema of the conjunctivas with mild edema and lacrlmation. The details of the iiis were slightly obscured due to some congestion. The latter condition improved overnight resulting in an average score of 7.3. Cfce animal was free of inflammation in 3eventy-two hours and all were given a score of zero in five days. 06-95 was classed as a mild eye irritant. DSW 586276 STLCOPCB4091610 Toi Monsanto Chemical .pany, . . St# Lewis, Missouri - Younger Laboratories Certificate of Analysis - Page 7 (2/22/58) - Y-58-U TABLE V HHALATION CF >03-95* VAPORS BY RATS Average Temperature Inside Chamber .......... Average Relative Humidity Inside Chamber . . ..... Amount Of >03-95* Vaporized. ............. 76.0 ?. 59.5 % 6.U Grams Animal Ilo. - Sex 1 - Male 2 - Hale 3 - Hole h - Male "ate du rvived 3w rvivod Gurvived Survived Cfoservaticns Luring Exposure Little banediata reaction -Activity about nornal Animals continue to exolora chamber - iscanfort slight after four hours. IT3CUSSION - The rats survived the six hour exposure with no aftermath of bronchial rales or other evidence of respiratory injury. Activity was not affected to any noticeable extent. The animals remained steady and were observed eating and drinking at intervals. Nasal and ocular irritation was not sufficient to stimulate a discharge* 6.U Grams, equivalent to 2.5 per cent of the sample, was vaporized. Considerable agitation was maintained at a pressure of three pounds per square inch. However, the chamber atmosphere remained quite clear with only a very slight haze detectable by reflected light# It was concluded that a concentrated atmosphere of OB-95 vapors was only slightly irritating to rats exposed for six hours# DSW 586277 STLCOPCB4091611