Document pD0VJNb2ZZqepEa8Mra4qMEw
AR 826 -- 0839
ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
EST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be
referred to as PFOS, FC-95, or as a component of FX-801. "`Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was cc 835-34. The test sample is L-9235 (FX-601). Current information indicates it is a mixture of 1% PFOS, 12% diethylene glycol butyl ether, 81.25% water, 2% propane Suitone foamer, 1% sodium octyl sulfate, 1% sodium decyl! sulfate, 0.1% N-(3chloroallyl) hexaminium chloride, 0. 8% xanthan gum, 0.8% starch, and 0.05%
tolyltriazole.
ErHon: Tchhaerafcotlelroiwiznegd scounmcmeantrryataipopnlsioefs tiompaurmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately
reflect the toxicity of the fluorochemical component of the test sample.
Method: Not given. Type: Acute static-renewalrange-finder
GLP: No
Year completed: 1984
Species: Pimephales promelas
Supplier: Commercial supplier, Brady, NE.
Analytical monitoring: Temperature, pH, and dissolved oxygen.
Exposure period: 96-hours
Statistical methods: LCso values not calculated based on study results.
Test fish age: Juveniles
Length and weight:
Average length = 35.4 mm
Average weigh=t 0.312 g (wet)
Loading: 0.52 giL
Pretreatment: 250 mg/L Tetracycline HCI.
Test conditions:
Dilution water: Carbon-filtered well water.
Dilution water chemistry:
Hardness: 250
mg/L
as
CaCOs
Alkalinity: 240 mg/L as CaCOs
Conductivity: 350 ymhos/cm
Lighting: Adaily photoperiod of 16 hours light and 8 hours dark with a 15to ~ 30 minute transition period was maintained throughout the testing period. Stock and test solution preparation: The test `material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Test solutionsrenewed. Stability of the test chemical solutions: Not noted.
002093
Exposure vessels: 1 gallon all-glass round tanks containing 3Ltest sNoulumtiboen.r of replicates: 1, except for highest concentration and blank
cNountmrboles.r Number
of of
fciosnhcepnetrrraetpiloincsa:te:fiv5e.
plus
a
blank
control
Water chemistry during the study:
PH range (0-96 hours):
Temper87a..0t8u---r88e..11ra((nc1og0ne0t0r(o0ml-g9e/6xLphooesuxurprsoe)s):ure2)0-22 C
Dissol6v.ed59o.xy4gmegn/rLa(ncgonetr(o0l-9e6xphoosuurrse)):
0.59.4 mg/L (1000mg/Lexposure)
RESULTS
-
Nominal concentrations: Blank control, 1, 10, 100, 500, and 1000 mg/L.
The blank control and 1000 mg/L exposure solutions were evaluated in
duplicate.
Element values: 96-hour LCs = >1000 mg/L
Element values based on nominal concentrations
Remarks: `Substance
TReesmtianrgkwsafsielcdo.nTduhcetveadlouensarmeipxotrutreed
as described applyto that
inthe Test mixture and
not
the fluorochemical proportion alone.
CONCLUSIONS T>1h0e0t0esmt sga,mptlhee9h6i-ghhoeustr cLoCnscoenfotrraftaitohneatdestmeid.nnoTwenwapserdceetnetrmomritnaeldittyowbaes observed in this concentration when the replicates are viewed as combined. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Rtseetslutidaoybrilglaiactnkyi:ssmaKsnai.lmyiTtsihcceahrlercaoinsnkfaiinrlgmaac=tkio3o.fncoThfahtreahcestteaurmdiyozuaistnitaoonrfoaffnlgteuh-oefriotncedhsietnmgsi,acwmaipltlhperloiapmnoirdtteitdohne" -- in the solutions.
REFERENCES
002094
TSth.isPsatuul,dyMwNa,sLacbonRdeuqcuteesdtbnyutmhbee3rMB1C6o8m4p,an19y8,4.Environmental Laboratory, OTHER Last changed: 6/26/00
002095
.
CHEMICALEENVNITCRTTYVONTEMISETN:RTALOSELUFNAMBMFAOEHRRCYATTESORREENYPVOARTLTUAATILON
chemtent ested 25 S(EX pMBS rent TyeeTHANG - EAE (EXLIGORT
Eoyrcso/icso/onso (Ov .u.): Yok AL GEELUELTANLIO023%
met Species LATEDANAS.{ TIHERAESTHAEBS)
Test Dates_/p =//- / L=1S2
LR. bi JRJB
Analyst(3)T
5
(Exposife/Test Conditions)
exposure Type:
static,
-
+ =-
SOtrabtiitca/lConShtaikniunogu,s
static Reclrculatory.
staticRenewaxZAlyzey Flow-Through
diluent:
-
Aerated viell Water (Initial 0.0. Z4/ma/1; pH_B,/+ Temp. 20 CULEE))
Water Hardness: A SO ppm HardnessClassification iA D
Dissolved Oxygen $ Saturation:
control(s)
Lowest Experimental Dose lmall
Highest Experimental Doselooowall
48-he 96-hr
Me.
qn
25
98
ET
EExxtpeonsduerde
----
pH Levels:
ashe Sehr EExxptoesnudreed
control(s) Lovest Experimental Dose lwgld
Highest Experimental Dosel000wald
B80
BO
78
80.
80
4S
Test Temperature C (F):
-
48-he 96-hr EExxptoesnduerde
ZocoK) zzz) te)
Photoperiod:
Sixteen hours light with a 15-30 minute transition period.
002096.
.
CR COA CNC
_SHIIC AEHCAUSPTRATORY RANAE -FINDER)
wr_ Ae SK
yee Tost Jr
material rested guns (bigs) tot CERN=SY
ot Sl
see 37 hussSiti
v5J,.520gr.ams avg. SizS e3, n No. Organiana pec doSsLeowd,ing Factor 0, SZ a
ish Disease Treatment2; S/) 411 / ledge AY
est: SolutionVolume, Stare/AllGlass Tank Tank Dimensions: Jeit-SoconncaesIHd
ime Started ///(JpaA testaM rted Y/Y 2 Date Campleted PVE
lution Water Carhon-filtered
ex_(ter. Etete
)
oul. sigA e
(95% Confidence Limits) >ag0u(s=
mg/l | added | Dead
oem| Dead
=
pm| Dead
ppm
lone = 7 bys]0blur]Ablibie]l kde
Olcowel
--1 dil bles] chdzd bd
Ll TeTo bili 0 bileal 0 big] hire
2lio sol bdr o bdsal oblr]0bdue
2 Licolwo| bpdoo BWA blidEdi
Lleol
o led] 2 aha claro bal,
5 liooolzeolbalsAo bell Chaar
hd
"licolgeol
0 Yoel Velo qd /TW 1 lek
Tee et Cr ICS 2X JUL QULALURITAPa Ril?ZIPP PLR Li2s LIQLD
scondary Effects (other than Mortality:)"
cso, BEST COPY AVAILABLE
=wo
nw wTmog mew.
mealfed
|TT]
[1IT C1 1
Z
2] T&F AEE
=1
I 1 1
# IScu| w 3b 1i 1 it ] y \
sa |2l 0 |As |20 a | 4 002
wou BLYEE
procureSten TEE GewpleOuiription
EnviroLnabm--eWonrktShaeelt ~~ po]
he2 d Toxo MTeER(CCG Yome:
ol
BLE | (Cue_mave|elosms ms
a
lnadel
ai 20 aH ans
2 [1/ G0887 .0|] =
002098
7-19-84
ENVIRONMEANTLALL RLESAUBLOTRSATOTREYNTASTTIAVTEUS REPORT
LAS REQUEST KO. B1750
QUESTER NAME: RRR
PT: 0222
OJECT NC: 06 TE RECEIVED:
6-14-34
SC: MAY-CARBON FILTERED WELL WATER
CONTRACT LAB: CONTRACT LAB COST:
0
3Y E-L HOURS: EST CWPLT DATE:
3 7-27-34
DATE COMPLETED:
SAMPLE DATE
CODE DESCRIPTION
TEST
RESULT
1
6-01-84 RME4
2
oI
WATER
PH RES=CL COND TOT-HARD 10T-ALK 1s NH3-N SOL-F 0D-LOW T0T-COLIF sec pd RES=CL COND sec
7.9 <0.02 MG/L 350UMHDS/CH2 240 MG/L 240 MG/L 320 MG/L #0.4 MG/L 0.2 G/L 4 MG/L NEGATIVE 49 PER/NL
6.8 <0.02
MG/L.
92.P1EURM/HNOLS/CM2
# = UPDATED DATA + = CONTRACT LAB
002099