Document pBr4yRaKy81j5yYBN97ap2ZZB

(JS ESTAI y S MI D i t o ; E . 1. d u P o n t d e N e m o u r s & C o m p a n y INCORPORATED W i l m i n g t o n , D e l a w a r e 19898 PO LYM ER PRO D U CTS D EPARTM ENT E X P E R IM E N T A L STA TIO N PERSONAL AND CONFIDENTIAL cc: A. J . Dahl B. W. Karrh L. J. Papa Pral File Tr - 353 - N11400 - 269 /575 May 19, 1981 TO: DR y. L. POWER - PPD, Washington Works FROM: S. S. STAFFORD ANALYSIS OF BLOOD SAMPLES FOR PERFLUOROOCTANOATE (Job No. 810-578; PRAL Nos. 81-1703-1706; Notebook Nos. E22514,E26238) As requested in your letter of 4/21/81 to L. J. Papa, the 4 blood samples submitted then have been analyzed for perfluorooctanoate (Cg) Results and sample identification are given in the attached table. As noted there, the analysis was done using a gas chromatographic method specific for C8 (Lab Method Number ES-567) but results have been reported as ppm F for comparison with total organic fluorine analyses. Precision is + 10% relative standard deviation over most of the concentration range, somewhat less at the lowest values. The lower limit for quantitation is 0.007 ppm F (0.01 ppm perfluorooctanoic acid), with a detection limit of n. 0.004 ppm which can be distinguished from the reagent background but not well quantitated. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analyses. General questions on blood sampling can be directed to J. W. Raines or L. F. Percival. Attachment jah Keywords: Perfluorooctanoic Acid Perfluorooctanoate Blood Analysis GC There s a w o rld o l th in g s w e re d o in g som ething about 000075 EXPOOOOll EID713825 TABLE I CONCENTRATION OF PERFLUOROOCTANOATE IN BLOOD (a) Sample PRAL No. 81-1703 81-1704 81-1705 81-1706 Date Sampled 4/14/81 4/14/81 4/14/81 4/20/81 P.R.Mo. 3854 4485 4457 4436 GC Analysis ( Date Analyzed tCr ], Ug F/g blc 4/24/81 4/30/81 4/30/81 4/27/81 33. 1.1 2.1 0.27 (a) Analysis as described in Lab Method ES-567 ("Determination of Perfluorooctanoic acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine ailalyses. (ppm F = 0.688 x ppm perfluorooctanoic acid) Estimated uncertainty is + 10% relative standard deviation. The lower limit for quantitation is 0.007 pgF/g. The detection limit is % 0.004 ygF/g, but concentrations in that range cannot be well quantitated and are reported as < 0.007. None detected (n.d.) is reported for samples with [Cg] < 0.004 ppm, which cannot be distinguished from reagent background. I I f *REDACTED* EXPOOOOl2 EID713826 00076