Document pBMm7wL03kz8a474xgELnam1B
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ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
TEST SUBSTANCE____________________________________________
Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-203FC. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample is FC-203FC. Current information indicates it is a mixture of 0.85% PFOS, 17% diethylene glycol butyl ether, 74.46% water, 2.34% acrylic foamer, 1.7% alkyl ether sulfate, 2.55% sodium octyl sulfate, 0.12% sodium nitrite, 0.09% sodium molybdate, 0.85% triethanolamine, and 0.04% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample.
METHOD:____________________________________________________
Method: OECD Guideline 203
Type: Acute static range-finder
GLP: No
Year completed: 1997
Species: Pimephales promelas
Supplier: Aquatic Biosystems, Inc., Fort Collins, CO.
Analytical monitoring: Temperature, pH, and DO.
Exposure period: 96-hours
Statistical methods: Not given.
Test fish age: Juveniles (128-132 days)
Length and weight:
Average length = 23 mm
Average weight = 0.09786 g (wet)
Loading: 0.2936 g/L
Pretreatment: None
Test conditions:
Dilution water: Carbon-filtered well water (3M Well #2, St. Paul, MN).
Dilution water chemistry: Not given.
Lighting: Not given. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Once. Stability of the test chemical solutions: Not noted. Exposure vessels: 1.2 L vessels containing 1.0 L test solution. Number of replicates: One. Blank control duplicated. Number of fish per replicate: 3. Number of concentrations: three plus 2 blank controls Water chemistry during the study:
001379
pH range (0-96 hours): 7.9 - 8.2 (control exposure) 7.9 - 8.1 (1000 mg/L exposure)
Temperature range (0-96 hours): 18.5 C Dissolved oxygen range (0-96 hours):
7.8 - 8.5 mg/L (control exposure) 4.7 - 8.7 mg/L (1000 mg/L exposure)
RESULTS___________________________________ ,__
Nominal concentrations: Blank control, 10,100, and 1000 mg/L
Element values: 96-hour LC5o = >1000 mg/L
Element values based on nominal concentrations
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS___________________________________
The FC-203FC 96-hour LC5ofor fathead minnow was determined to be >1000 mg/L.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY _____________________________________________
R eliability: Klimisch ranking = 3. The study is a range-finding, with limited exposure concentrations and test organisms. There is a lack of documentation. The sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES________________________________________________
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number R1335, 1997.
OTHER_______ :________________________________________________
Last changed: 6/26/00
0013S 0
TYPE TEST:
ENVIRONMENTAL LABORATORY
96-HR ACUTE TOXICITY TO FATHEAD MINNOW, PIUEPHALES PROMELAS
STATIC V
STATIC RENEWAL ______
LR R 1>335 PB---- 1-- o f 3 .
R lV O G E FvOOC
TEST SUBSTANCE:
fC ~ c F C
A F r r ___________________________
DILUTION WATER:
CARBON-FILTERED WELL WATER OM WELL 12. ST. PAUL MW T w n p .______ C ,p H _______, D .O ._______ m g /L
TEST FISH SOURCE:
DATE RECEIVED: 9 . - I S - < n
FISH AGE:
AVG. WT^O-P^ f e .O ^ t g
AVG. SIZE: 3 3 0 3 cm
NO. OF F1SIVTEST CHAMBER:
LOADING RATE:
D ?930>
TEST SOLUTION VOLUME:
/ OL
SIZE OF TEST VESSELS:
DATE INITIATED: 9 - 1 ^ - 9 1
DATE TERMINATED:
U 5Q T<Cs
TEST INITIAL TOTAL VESSEL CONC. AMOUNT 24-Hfl
mg/L ADDED ----
C> o
pH
1,1 1.9
D.O. 48-HR
mg/L
~r.V T.l
o O
MORTALITY pH D.O. 72-HR pH
mg/L
T-o 7.R *.o
o 0
& .D 8\
D.O. 96-HR
mg/L S'
O o
pH %.T-
D.O.
mg/L 7 .g _ YM
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TEMP. C ` Y . S 0 DATE / ANALYST
LC50 (95% C.l.)
l l o o A iy \'T<c.S
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SECONDARY EFFECTS: S Surfacing; E * Los of Equilibrium; Q * Qulascant; R Erratic Swimming; H - Hemorrhaging
ANALYST:
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BEST COPY AVAILABLE
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Environmental Laboratory
Lfl: Date: * K lr - r > Initials: _ 2vts_____
Page jL of 2
96-Hour Acuta Toxicity To Fathead Minnow, Plmaphalaa Promalm
Loading Rate Data Sheet
Teat Subetance:
<^o3 F c ^fT F"__________________________________
Flah # 1 2 3 4 5 6 7 8 9 10
Average
W0 e.igah^t,iu(,g)
O.OJxz
O 12-33 O. 11M*4
o.of iz
O. O
O0..Oo(&o('osa1 O.o^toH . .0 2 0 1 1
Length, (cm)
ra-H
1 a-*
ao
2. 2-
2.S 2a..2L. 3.3
L o ad in g R a te C a lc u la tio n :
Avg. wt. (g.): O. Q9~?S>,fe
Std. Dev:
OO
No. fish/vessel: >3______
Vol./vessel:
( ______
o ^ 2 3 l M g . fish 1___ L
O !Loading rate: % 5 <0
:t y .
'iff;
i'H-
w `'i
-?:i f >
0 Q lJ & 2
