Document p2vk4km1L57x8J8Ja98xx8zKk
AR R26 -- 088
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
IESTSUBSTANCE
Identity: rMeifxetrurreedctoontaasinPiFnOgSp,erFfCl-u9o5r,ooocrtaansesauclfoomnpaotneewnhticohf mFCa-y30al0s3o.b(e1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: is FC-3003.
The 3M production Current information
ilontdincuamtbeseritiwsaasmnioxttugriveeonf. 1T%hePFteOsSt,sa1m5p%le
doicteytlhysluelfnaeteg,ly1co%l sbuotdyiluemthdeerc,yl79s.ul3f9at%e,wa0t.e0r,6%2%soSduiiutmonleaurfyolamseulrf,at1e.,5a%ndsodium
0.05% benzotriazole.
cThhaerafcotlleorwiiznegd scounmcmeantrryataipopnlsieosftiompaumriitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect toxicity of the fluorochemical component of the test sample.
METHOO
Method: OECD Guideline 209
GLP: No
Year Completed: 1997 Analytical monitoring:
Dissolved
oxygen
concentrations,
temperature,
and
pH. Statistical
methods:
Results were
determined
by calculationofthe
%
Tinehsibtitoirogn ainn irsesmpisroautirocne:ratAec.tivated sludge mixed liquor collected from the
Metro Wastewater Treatment Plant, St. Paul, MN.
Test condition: Dilution water:
Carbon-filtered well
water (3M well #2,
St.
Paul,
MN)
RSyenftehreetniccesaenwdagtees:t spoelruOtiEoCnDprmeeptahraotdion: A stock solution of the
5re0f0ermegncien s1u0bsmtLan1cNe,N3a,O5H-,didcihliuotreodpthoen3l0,mwLawsitphreMpialripeodrebyMdiilsis-oQlvTMing
`awnatderd,iltuhteend tboro1uLghwittthoMtihlelp-oQinTMtwoafteirn.cipTihenet pprHeocfipitthaetiroenfweirtehnc1eNsoHlzutSiOo4n
was measured to be 6.3. Test substance was added directly to the test vessels.
.
TNeusmtbveersoseflcso:ncNeonttrgaivteino.ns: five plus blank control
TToetmaplersautsupreen:de1d9.s2o--li2d1s.6anCd pH on day of testing: 4.04 g/L (TSS in
final 1.61 Element
Bg/aLs)i,s:initRiaelsppiHra7t.i9onanidnhifbiinatliopn Has8.d5etermined
by
oxygen
consumption Remarks: Values
corrected
to
20C
for
calculations.
002702
Reswts
TNohmeibnlaalnkcoconncternotlraantdio1n0s0:0 Bmlga/Ln.k wceonrterodlu,p1l0i,ca5t0e,d 100, 500, and 1000 mg/L. Element values: 330--hmoiurnuEtCe5E0C5=0>1=0>0100m0g0/mLg/L
Element values based on nominal concentrations.
Remarks: Substance
RTeesmtairnkgswafiseldc.onTdhuectveadluoens
trheepomritxetduraeppalsydteostchraitbemdixitnutrheeaTnedstnot
the fluorochemmical proportion alone.
conclusions Tdehteertemsitnesdubtsotabnec>e130-0h0oumrg/ELC.sTfhoirsaccotnicveatnetdrastliuodngienhriebsiptierdatrieosnpiirnahtiibiotniobnywas.
~24%.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St.
Paul, Minnesota, 55133
patAQuAUTY Reliability: Kiimisch ranking = 2. The study meets all the criteria for quality tleasctkisnga.naHlyotwiecavlerc,onsfairmmpalteiopnuroiftythweaasmnooutnptroofpefrlluyorcohcahreamcitcearlizperdopaonrdtitohneisnttuhdey solution.
REFERENCES "STth.isPasutlu,dyMNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMR1C3o3m1p,an1y99,7.Environmental Laboratory,
OTHER Last changed: 6/26/00
002703
ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MXED LIQUOR
wDanteR3-[2313]-37 Analyst TICS Page 1" of 8
Type Test QECO ACTIVATED SLUOCE RESPIRATION TOHIGIT 00 TesTH* 2
ACTIVATEDSLUDGEMIXEDLIQUOR
Date Obtained 3-27-97
Date Used3:2/-97
Mixed Liquor Suspended Solids (MLSS)4-24_ gi Adjusted to __= git
Ie 3lzdTTes MLSS in final test solution $6 g/L
.
Ad
Initial pH _1.Q_ (1st test soln. prepared)
Final pH _ (last soin. prepared).
comments _____
IESTSUBSTANCE
Sample Description FC~ 3003 AFFE.
Initial PH of highest conc. tested (COOMRIL) = la
Adjusted to pH =
Comments
EE
"REFERENCE SUBSTANCE
sample Description 3,5 - Dichloropheno
Initial pH of stock solution (SCO _mgll) = _G3
comments
i
Adjusted to pH _
[EXPOSURECONDITIONS
2
Test Ouration _3 hows
=
Test Temperature Range: 132-216
Comments
mm ---- rr -- ------------
002704
ENVIRONMENTAL LABORATORY
wPai 2otz F
.
OECD ACTIVATED SLUDGE RESPIRATIONINHIBITIONTEST 200
TESTSOLUTIONS PREPARATION
Tost substance: _FC= 3005 AFFF Sample Description: _ge\den tonguon Vigu.d
Analyst: _ VES . paw _321-97
TestConc.
_mol
mgadded
perS0oml
wer FHT
mtmCthwater mLsyn. sewage `mLmicrobial
_pecSOml feedper500ml Inoculumper 500mL.
TotalVolume.
--
pio = md Te
eo
goo
-T aso d ia z s o sco
So
2S
azq
lo
200
Soo
c70s0
50 259
=
20
50
=)
250
259
Tecoseo @gd
Io
Te
2D
Sco
wm sco
TG mo agg Te
ae
-_----
Tut af QR of
bighest Cone. eded= Te
s00
-
-_--
(1) M3l-i2p1o-r2e1-MTietgecigh-pun-saiar
a
oC8
002705
BEST COPY AWAILABLE ENVIRONMENTAL LABORATORY
LaY R12T 3
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
=
RAW DATA
Tost Substance: FC~3C08 AFEF
Source of Activated Sludge Mixed Liquor: eden(Dasle(ferTrendment
Date Received: _3_~R 1:97
Date Used: 2-21-97
h
?oAo. g
m
Analyst:
Tvs
30-MINUTE EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, moll
.
Test Substance Concentration (glo) 0.5
TMEMINUTES
--
2.0 (2.5 |3.0 35 [40 [45
Tremp 55]
=lel22llena|oanl seesau l 4a a4laa]]aasafas le io oi
Teilels]lecolsSesldsg1||44sa]l41oll3ascsla3ayllaa slaatlpnll
Au a:
Toc co
les] [63]
6255]4a]43 dx eo ss4a dasa]
3a 3a
agladl13[ [al anfaal alial
1. Jos
Toco Toco
[5% [SE
a oai|4l3434a(l29s2e4[lza o[l o1aallla oesssll= = li =e
--=1321o Tazo
4alsn valu roloe 20 BKooNTROLT
[voso[galjase [15 [20[es[ed]sal sal 10a 3-HOUR EXPOSURE
Test Substance
Concentration(og)
DISSOLVED OXYGEN CONCENTRATIONS, moll
x
TIME MINUTES
10)15)20 25) 30]35 40 45]
:
TT
5.0( 55
Tew
c
BKCONTROL |
1
oc _ 50
[34083]gos[1 Gt CL erie ad] [52 [188 [195 ] eI3 65161 .2]
26.0 19.5
S70o0ola[ fz8 xe. [e A 1 r | yT[7e.a0T]e6s36eda][o Scs iet n 5o3n|fi5a01
19.9 133
ZooTray]Ee XD[ge 8A[8| 4
o731te4[Lo7t0] Cex4[6S[]56 .%[a 5 sd1
113 TY
woamait lagae|raf ex] es] sal ss] sale] 4s]39 |202
BHE fost
of
teamcptaievsratatetSduormesei:a
na1ti9oi.nxe2ds2lti1equd:o:r
:
Inianl1 it1. .i3 a9l
FEinrala. B4--
;y
0021706
]
Lo CAOUATONOFRESUTS >5 TAonoatlySsutb:sTtanucey: FSGlu3n0t0o0a_tAeFrFE gz ute Exposure
`ReOsEpCiDraAtcitoinvaItnehdibSiltiuodnge Test #209 Oiasoed OxygenConcentration,my
Oate: 3121/07 5"
!
3 8
[liaxer[f7a6r1e7s5[6eo7[[s6e2|6s18la6s4tl4so [so[7 [os[et[5s[4s ea ar
a4s2[a9r0120s2 os onl2s
2200]soutss[[10o0e06sfoosseorrill o tol are[10670 fossa] o
|||
[[[ooSooooo]]"sssass[|[eeszo2[[|s54s5a [[[oeaooa[[|424320[[2a0874[1283042[22t7si1[l22o2as|lvve7|l11Taaa2t210o10[||tt1eo0rt8ee5s0sf[[orrsiorsteezosa]l]0o0]||
[loo%koeGoTi]nssceo||nas5s.(10[.[445s.23 [o5r6[28s0O 22s7T ]2te4ltooslftoasl| v0|]os| aeroos[Lovsearssfaurcsamerar(os0l].--0--6472]|
sou x
Dissoed OoygenConconratons, mp
[[[soBokll1a0er0aeeesrloeas1o[77oszs|777as.16r6e70l6eros1la6essslseseirll|assnar]lssaaaolll2or0oo0s[|[ooorrmoeeronetfufofoeosasasosenall]oo |||
[[[0oGoo0oo][lsoeea[aezsao[77e7os[[[777.614[l]7e75r0l76e0ss]166674off66s0s4[]sesizr|[|sa5a8rl|sssaoollltttooosso[[|ooosenssueesrsJ[oocnsooassnar]}]itosasso|||
C[of%xof0emo[]c7o8n.9v6[c[lo7e.64(6[7167.2214[Te2s01[67.5[0[C75.40T1|T7650y[5ee.as1--[1"64--56[[4e3115a00]2r0o5s[[oosermseFf[aoocesm(aee0]l92208251|
@0 faeeCnopaenttearroneogpcnam)
"3
0rceen carce:rPRIDIRG (T t w 5of h
3 toSsnnce it 0: enn Tans
ENVIRNOMENTAL LABORATORY wmfRy1r3o3r)an OECD ACTIVATTEESDTSLSUODLGUETIROENSSPPIRRAETPIAORNATIINOHNIBITION TE#S2T00
Test substance: 3,5 Dichlocophenol sample Description: ALDRICH (Reo LABEL) LoTTM 096ISKKG DE
Analyst: TS
_ omw 3:21297
Stock Solution
Preparation:S20 Le
z0."
SOise solun ed
S.S5e -Dcf Re mine
T
a 33 8, nN : ie40 in ;
A Td aSek sle s e TTT
TesCone. miukied moO)3W3ik mLaynsewage
--med_ erS0mb perS0OmL feed perS00 ML
mL microbial Tora Volume
inecuySmOoOmelr. ml.
Buono ETET EE EXET)E
5
Co
io
AQ
PE
274
le
-- re
| Tefii? aPpl4lWhgigbehesstccoonne deeded2= 7138
200
repiprm--
200.
eee
S02a.
op
OY E T
m ---- T eee --
--------ee,
-- -- - _---- M -- e A -- ----_-- ----A=
mm
= 5
_--
(1) Mitimere--tit-0R-igh-puriswaier
T&S
3-215
a
=
002708
ENVIRONMENTAL LABORATORY
LA_R 1331
Pq60% 2
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance: 3,5-03chloroghenol
Source of Actvated Siudge Mixed Liquor: Mheko (Waste(ater Treatment Plait sb
Date Received: 3-2/ "97
Date Used: 3-21-97
Analyst:
{ xs
30-MINUTE EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mg.
TCoosntceSnutbrsattainocne(gl) 1.0
2T5I5MEMI[3N.0O|TES3.5 [4.0 [45 [5.055] Temp
BKeoNTROLT
o
ooNTROLT
[or[delta salsa[an[29 a[ra[rooe [are [ez] [ 76 [7.38 [Ga 7|7 C22 6[3SY[. 61]e9 f|e42o[7 S51356 228 |350late rrrrr r errrrr EL rrr rrr rrr
3-HOUR EXPOSURE
TestSubstance
Concentration (glu)
DISSOLVED OXYGEN CONCENTRATIONS, moll.
1.0 | 1.5
TIME MINUTES
Temp
25)30| 35 40 45| 5.0) 55 oc
BKCONTROLT To
SxcontRout
[ei]
B
90 | % 8 $218 |75 |70 lbs
$0 |S3 [192
Ho l39[ay[ws iu wats [37 [7S[0[72 | 20.1
[T 1 --TTTr
]
rrr
_
TT T 1 [r | t CTT =
ba l7e [72 [c8 lez sq [ss [1 | [42] 39 [203
PPHH ooff hacitgihveasttedconscleundtgreatmiioxnedtesltieqduo:r;
Test temperature: _19.2=2).G
IInniittiiaall1 &. -9 6
FFiniaS nalq _86
00270
ENVIRONMENTALLABORATORY CALCULATIONOFRESULTS. .
ROeEspCiDriatAcitoinvaItnehdibSiltiuodnge Test #209
TesTtSruobsetancer:eyL.5Dishoraghanal
2 `Analyst: Tuudy Stisfhostor
30Minute Exposure.
Dissolved Oxygen Concentations, mp
Date: E32L1V197 .
?3x
?22
po ree feresfereelperealjeeeeoop fTooSue Tla4ee sT[eaponfe oao7[lesoas[l6z0al|a21 v1s0|[ooososiw-- saJjoossesm-- ]}50 |
FH H Ht ++ tt -- -- ++ --r T+ r +r r T r --T T rT T r 1r--r1
bF etet restattss+rerstTarT ts alotos|moLos laemee(a5l0.8642
= Gf in covos (0: 53
3H.oeunrEExposx ure pens Dissolve ed Orygen SConceS ntratioe ns, m eTOTea a
fofeetm esfem stosjHe aE tliM otN m FaTsNt lE oi or rE [sa|MtTT zX|oCmXmT TatHJEoEssia} |
F H H-- t t--r 7-- 1+ t r r tr T--T
FH FeeHt--sta+ts+at+estr estH T eats orlasr Lasr LosT [waLtorsesFlaocsma(tl0.8o243d
0 aifoIrn ncon nvo (f4on 1 2e4k)
@00fomthoea(spnGp-.aP aELmIR (1 rti wn 15 fhch)
0 eanpsapme crn Par: tkct50 st
Tazo
ChainofCustody Record n E MeRirS
ns
07688, p = rey 1]
wm ny Tf
f pS te p p] e
El ewe [Hee |am]
|
He a 5 -N meli D[ Eco -- mme
Cr ER 0 57 CE X=
Gree
ee
1
5 or-- i
rr ree
rr
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| 1
3 orrt
br ree
1
a br
ee
v
rr ree
Cr ree
re
nr ree
rr ree
Grrr rrr
he nmr R=97/7/73 FTN 717300 / AB Fh GR I SWE ke TL
r te v ete| r o o T T
rr rt
rT 1-- 1[1