Document p2eOrVD9NkvYBgzLVrNvKg52X

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SU 3STANCE Identity:Mixturecontainingperfluorooctanesulfonawtheich may alsobe referredtoas PFOS, FC-95, or as a component of FC-203FC. (1Octanesuffoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted.The testsample isFC-203FC, identifiebdy the laboratoryas R1335. Currentinformation indicatesitisa mixtureof0.85% PFOS, 17% diethyleneglycolbutylether, 74.46% water,2.34% acryiifcoamer,1.7% alkylethersulfate2,.55% sodium octylsulfate0,.12% sodium nitrit0e.,09% sodium molybdate,0.85% triethanolaminea,nd 0.04% tolyltriazole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately retlecttoxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline203. Type: Acute static GLP: No Year completed: 1997 Species: Pimephalespromelas Supplier:In-houseculturesm,aintainedatAScl-DETD, Duluth,MN. Analyticalmonitoring: Temperature,pH, conductivitayn,d dissolved oxygen. Exposure period: 96-hours Statisticamlethods: The LC50 valueswere calculatedby Trimmed Spearman-Karber method. The NOEC valueswere calculatedby Fisher's Exact testusingToxstatstatisticsaolftware,Version3.5. Test fishage: Juveniles Length and weight: Average length 16.6 mm Loading: 0.12g/L Average weight 0.036g (wet) Pretreatment:None Test conditions: Dilutionwater: DechlorinateCdityofDuluth,MN tapwater,post- carbon filterT.he waterwas aeratedfor24 hours priortothe test. Dilutionwater chemistry: Hardness: Alkalinity: Conductivity: pH: 48 mg/L as CaC03 44 mg/L as CaC03 119 pmhos/cm 8.08 LightingC:ool-whitfeluorescelnitghtsA.dailpyhotoperiodf16 hourslight and 8 hours dark was maintainedthroughoutthetestingperiod. Stock and testsolutionpreparation: The testmaterialwas added directly tothe dilutiownater on a weight/volumebasis. Concentrations dosing rate: Once. Stabilityof the testchemical solutions: Not noted. Exposure vessels: 4 L glassjarscontaining3 L testsolutions. Number of replicates:2 Number of fishper replicate:10. Number of concentrations: fiveplusa blankcontrol Water chemistry during the study: pH range (0-96hours): 7.68 - 8.08 (controelxposure) 7.45- 7.81(1000 mg/L exposure) Temperature range (0-96hours): 20.5- 20.7'C Conductivityrange (0-96hours): 119 - 141 pmhos/cm (controelxposure) 119 - 143 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-96hours): 6.9- 8.7 mg/L (controelxposure) 5.1- 8.7mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control,130,216, 360, 600, and 1000 mg/L Element values: 96-hour LC50 = >1000 mg/L 96-hourNOEC = 600 mg/L Element valuesbased on nominalconcentrations Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLU&I-ONS The FC-203FC 96-hour LC50 forfathead minnow was determined to be >1000 mg/L. The 96-hourNo Observed EffectConcentration(NOEC) was 600 mg/L. Submifter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2. The study meets allthe criterifaorquality testing.However, sample puritywas not properlycharacterizedand the study lacksanalyticaclonfirmatioonfthe amount offluorochemicalproportioninthe solution. REFERENCES Thistestwas conducted by AScl Corporation,DuluthEnvironmentalTesting DivisionD,uluth,MN atthe requestofthe 3M Company, Lab Request number R1335,1997. OTHER Last changed: 6/26/00 ASciCorpomtion/AScl-Duluth EnvironmentaTlestingDivision AScl StudyID# $010-068 STUDY TITLE ACUTE TOXICITY OF R1335 TO FATHEAD MINNOW (Pimephalepsromelas) DATASTANDARD OECD GUIDEUNE 203 STUDY COMPLETED June 26, 1997 TESTING FACILITY ASel Corporation/ASci-Duluth EnvironmentalTestingDivision 4444 AirparkBoulevard Duluth,MN 55811 Tel.No. (218)722-4040 Fax No. (218)722-2592 STUDY IDENTIFICATION NUMBERS ASCI Study ID# 5010-068 3M Company Lab Request#R1335 Sponsor: 3M Company Sponsor Study ID# R1335 1.0GENERAL P*'ORMATION ASciCorpomtion/ASci-Duluth EnvironmentTaelstinDgivision ASclStudyID# 5010-M Study Title DataStandard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility PrincipalInvestigator Project Director Testing FacilityDirector DefinifiveTest Dates Data Validation and Report Review Acute Toxicity of R1335 to Fathead Minnow (Pimephales promelas). OECD Guideline203. 3M Environmental Technology and Safety Services,Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. Susan Beach, 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. R1335. AScl Corpomtion/AScl-Duluth Environmental Testing Division, 4444 AirpaTk Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Joe DieTkes Joe Amato Donald Mount June 17-21, 1997. Alan Mozol R e rtS*ignatur Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study iscompleted. AScl Corporation/AScl-Duluth Environmental Testing Division,4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-404:0. Sponsor-3.M CompiLny SponsorStudyID# R1335 2 2.0OBJECTIVE(S) ASCICorpomlion/ASel-Duluth EnvironmentTaelstinDgivision ASelStudy[D# 50ID-WS To determinethe96-hour10% and 50% effectconcentration(sLCIO and LC50),and the96-hour no observableeffectconcentratio(nNOEC) ofthetestsubstanceR,1335, forfatheadminnow (PimePhalesPromelas)under statitcestconditions. This studywas conductedaccordingto theAScl studyplan retainedby theSponsor and OECD Guideline203 (OECD 1993). 3.0 TEST METHODS TestSubstance erties:R1335, orange-yellolwiquids,pecifigcravity= 1.1(water 1),stableand misciblewithwater. Test Organisms Testconcentrations0: (dilutiownatercontrol)1,30,216, 360, 600, and 1000 mg/L. Test solutionpsreparedby mass additionof testsubstanceto. 6-L aliquotosfdilutiownaterfollowedby vigorousstirring. Source: Healthy,juvenilefatheadminnows (Pimephalespromelas) obtained from AScl-DETD in-houseculture. HoldinglacclimalionT:he fishused inthetestswere obtainedftom AScl's in-houseculture.The fishused in thestudywere hatchedbetween May 17 and 23, 1997. For the48 hourspreceedingtestinitiatimoonrtalitwyas 0%, and thefishwere notfedfor24 hoursbeforetestinitiationT.le fish mean lengthwas 16.6 2.5 mm, and mean wet weightwas 36 19 mg. Loading ratewas calculatetdo be 120 mg of fishper L testsolution. DilutionWater Use in test:Used 10 organisms/replicaatned 2 replicates/test concentration. Source: DechlorinateCdityof Duluth,MN, tapwater.The most recent chemicalanalysisisin Raw Data Package. CharacteristicsA:t testtime,thewater had a hardnessof 48 mg/L (as 1 CaC03) and a pH of 8.08.Aeratedfor24 h priortouse inthetest. Sponsor. 3M Company SponsorStudyIDO R1335 3 ]Exposure Chambers Incubation Observations Endpoint Calculations 5 ASciCorporation/AScl-Duluth EnvironmentaTlestingDivision ASci StudyID# 50iO-068 7ype: 4-L glassjars. Use intest:3.0 L of dilutiownateror testsolution/chamberD.uring the test,keptcovered,exceptwhen experimentalobservationwsere made. Solutionswere not aeratedduringtheexposures. Duration: 96 hours. Daityphotoperiod: 16 h lightand 8 h dark, maintainedusingcool white fluorescenltamps. Temperature: 21.00 10C. Biological:Daily- Test organismswith abnormal behaviorand signsof stress,and dead testorganisms. Water chemistry:(1)At testinitiatiotno-talhardnessand alkalinity(,2) daily-temperatureD,O, conductivitya,nd pH. Alldeterminationwsere made accordingtoAPHA methods (1995). LCjo: InhibitioCnoncentratio(nlCp)Method. LC50's: Trimmed Spearman-KarberMethod. NOEC: Fisher'sExact Test(TOXSTAT statisticsaolftware,Version3.5, West _Inc.a,nd Universitoyf Wyoming). 1 Sponsor. 3M Company SponsSotrudIyD#R1335 4 3.0 RESULTS ASci Corpomtion/ASel-Duluth Environmental TestingDivision ASel Study IDI 50IG-069 TestSubstanceToxicity(Pertinendtataare in Table 1) 24-h LC50: > 1000 mg/L (Not Calculaabbllee)). 48-hLC50: > 1000 mg/L (Not Calculable). 72-hLC50: > 1000 mgll (Not Calculable). 96-hLCIO: 116 mg/L (NotReliable). 96-hLCSO: > 1000 mg/L (NotCalculable). Ranges of Water ChemistryParameters 96-hNOEC: 600 mg/L. Totalhardness(asCaCO3 mgll): 46-48. Alkalinit(yasCaCO3 mgll): 44-48. Temperature(OC): 20.5-20.7. DO (mglL): 5.1-8.8. pH: 7.45-8.08. kecificconductivit6yilMos@Lm2:119-143. _j Sponsor: 3M Company Sponsor Study ID# R1335 5 ASciCorporaLion/ASci-Duluth EnvironmentTaelstinDgivision ASclStudyID# 5010-MB 4.0TEST QA/QC QA/QC Criteria Mortalityincontrolexposuremust notbe more than 10%. Data 0% mortality. The lowestDO concentratioant test terminatiomnust be at least60% of theair saturatiovnalueattesttemperature. Testdurationmust be 96 h. ReferenceSubstanceToxicityTest(thistestis optional). The lowestDO concentratiomneasured was 57% of theairsaturatiovnalueat21.0*C. Thisvalue was measured at testtermination forthe1000 mg/L concentrationT.his sole valuewas below 60% saturationD.O depressiondid not appearto significantalfyfpct thetoxicitmyeasurement forR1335. The testdurationwas 96 h. Controlchartforsodium chloridereference toxicittyestinigsin Raw Data Package. 5.0 REFERENCES American PublicHealthAssociatio(nAPHA). 1995.StandardMethods fortheExaminationof Water and Wastewater.APHA, Washington,D.C. OrganizationforEconomic Cooperationand Development (OECD). 1981/1993.OECD GuidelinesforTestingof Chemicals.OECD PublicatioInnformationCenter,Washington,D.C,,, Hamflton,M.A., R.C. Russo,and R.V. Thurston.1977.Trimmed Spearman-KarberMethod for EstimatingMedian LethalConcentrationisnToxicityBioassays.Environ.Sci.Technol.11(7):. 714-719;Correction12 (4):417 (1978). USEPA. 1993. A LinearInterpolatiMoenthod forSublethaTloxicityT:he Inhibition Concentratio(nICp)Approach. Version2.0. West Inc.,and Universitoyf Wyoming. Toxstat Software Package. Version 3.5. Cheyenne, WYO ming. Sponsor: 3M Company Sponsor Study ID# R1335 6 AScl COrPOmtion/ASel-Duluth EnvironmentalTestingDivision AScl StudyID# 50104M TABLE I. ACUTF, TOXICITY OF R1335 TO FATHEAD MINNOWS (P.promelas) 0 (control) 130 216 360 600 1000 Percmtagts am inpamnthesis. Cumulati@eNumber of Dlead Orgwtisms ind:Percent @24 b 72 b 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 1 (5) 0(0) 1 (5) 3(15) 5(25) j 5 (25)= 0(0) 3 (15) 2(10) 2(10) 2(10) 5 (25) LETHAL AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFI]DEXCE INTERVALS NOEC 24-h LCSO 49-h Lcso72-h LC50 96-H LCIO 96-h LCSO 96-h NOBC Values based on nominal testconcentrations. Data (ma a > 1000 > 1000 > 1000 116 > 1000 6DO 95 % coli Not calculable Not calculable Not calculable Not reliable Not calculable Not applicable Sponsor. 3M Company Spon &orStudy ID# R1335 7