Document p2Rz2O07YoxJvM33B2ek4e7Jd
BIOCONCENTRATION (DISTRIBUTION COEFFICIENT)
TEST SUBSTANCE
EHR - 0800-373
Identity: rNe-fmeertrheyditpoerafsluNo-rMooecFtOanSeEsAullcfoohnoalm,idFoCe-t7h9a0n,olo;r mFMa-y3a9l2s5o.be(1-. Octanesifonamide, N-methy-1,1.2,2.3,3.4,4,55,6,6,7,7,8,8,8-
heptadecafluoro-N-(2-hydroxyethyl)-, CAS #24448-09-7)
Remarks: Material is an off-white, waxy solid of uncharacterized puity.
METHOD
s
Method/guideline followed: 3M Environmental Laboratory protocol ~~ & +,
Type: n-Octanol/Water distribution
rg
GLP (VIN): No
w oi
Year: 1979
RB a
=;
Test Temperature: ~22C
Cor
A ree TRY #
wRaesmaurskesd f"iaesldr:ecTeeisvteidn"gwwitahsoudtocnhearoanct3erizPartoidonuoctfiopunriltoyt.505. The sample
Two concentrations of N-MeFOSE alcohol in n-octanol were prepared; Otonceoamtpe1n0s0a0tmegf/oLr aanldacoknien aatna2l0yt0i0camlg/pLr.oceHdiugrhescosnucfefinctireanttliyonssenwseirtieveusteod
detect the N-MeFOSE alcohol in the sample re-extracted from the water phase into ethyl acetate.
: 25 mL of each solution were added to amber glass jars, each containing
900 mL of deionized water. They were shaken for approximately 16 hours
at which point the octanol phase was drawnoff and the water phase was
p1l3a,c7e0d0xinG2f5o0r 1m5Lmpionultyecsa.rboWnaatteer-cpenhtarsiefuagleiqtuuobtessfarnodm eceancthriwfeurgeedtahten tarnaynsofcetrarneodlbcyonptiapmeitntahtrioonu.gh Tthwoe sseapmaprlaetsorwyefruenntehlesn teoxitnrsaucrteedrethmroevealtiomfes wcihtrhometahtyolgarcaepthayt.e, concentrated, and analyzed by electron capture gas
RESULTS
CNo-nMceenFtOraStEioanlcionhtohle/wna-toecrtapnhoalsseoslustihoanksewnewrieth0.10010809ppppmmaanndd200.00033ppppmm
respectively.
n-Octanol/Water Distribution Coefficient: 1000 ppm test solution: 52,900
EPA-OTS
RNB
000811809R
.
n
*
"ih
006533
2000 ppm test solution: 60,600
Average:
56,800
CONCLUSIONS
This data indicates that N-MeFOSE alcohol has the potential to bioconcentrate in living organisms.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Reliability: Kimisch ranking 3. While this study met with the standards at the time, the data is not reliable. Test substance lot 505 purity was not properly characterized. Validity of the analytical method was not evaluated, hence, the limitof detection / limitof quantitation, reproducibility, etc. are unknown. Because the solubility of the test substance in n-octanol is not known, the concentration of the test noctanol solutions may exceed this limit. No attempt was made to analyze the n-octanol phase for the fest substance. tis better practice to analyze both the octanol and water phases. This was done in previous attempts to measure octanol/water partition coefficients at lower octanol concentrations and the N-MeFOSE alcohol concentration was not measurably lower in the octanol phase. The procedure itself lacked the ability to compensate for potential losses of the material through sorption onto transferring vessels (e.g., the pipet and separatory funnels). A characteristic which is now understood. Calculation error in reported noctanoliwater distribution coefficient. Result should read 56,750 instead of 56,800. Another significant limitation of this study is that it used cnoonmsppoenciefnitcsanianlNyt-iMcaelFtOeSchEniaqluceosh.ol.AddOicttiaonnoallliyw,attehrerpearatrietiaotn lceoaesftfi3cimenatjsor should be measured on pure compounds.
REFERENCES
3M Technical Report "Distribution Coefficient of FM 3925 in nOctanol/Water." E. A. Reiner, Project 9970012600, Premanufacturing Notice FC-790, Report Number 015, January 10, 1979
OTHER
Last changed: 5/17/00
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3m
| Formerargra
TECHNICAL REPORT SUMMARY
70: TECHNICAL COMMUNICATIONS CENTER -- 201-20
(Important -- porespied onbotsides fpar,sendtwocopi0TCeC.s)
"
1/10/79
= "Environmental `Laboratory (E.E& PC)@&
Premanufacturing Notice FC-790
c05l35l
9970012600
Distribution Coefficient of FM 3925 in n-Octanol/Water
015
V. Pothapragada
E. A. Reiner
47816
48838, pp. 6, 8, and 9.
SCURTYD C08, onpgmi oBanoinion) | "mene
Beret Reed
AEDS, ro
Dime | Do determine the distribution coefficient of FM 3925
between n-octanol and water.
DCiosattrtiibcuiteinotn
PCoaertttiitciioennt Bioconcontration EenTsRsETmRAeCaT,nF BRS o EomS Thr aA p eas eyTa Comrie Go
Fluorochemical | The distribution coefficient of FN 3925, lot 505,
between n-octanol and water was measured according
p2tr0oocbaeedmu5or6de,i.8f0i0c.aThteioTnhdiisosftrritebhsueutlitEonnvsiurgcogoneesmftefsnitcaitlehnattLawbtaohsirsatdomeretyveerrmiisnled v
is likely to accumulate in aquatic organisms to levels above the ambient aquatic concentration.
HEs,)
006525
.
on
Introduction
Lacicvuimnuglaotreganliispmosphiploiscsesosrgathneicabciolmiptoyundtso eciotnhceerntrdaitreectalnyd from their environment or from their food source, a phenomenon which is well document (1,2).
cInoef19f7i5ciNeenetlyc,anetbe aluse(3d),todeesmtoinmsattreatetdhisthabtioctohencepnatrrtaittiioonn epostteinmtaitael.biocAsoncaenrtersualtti,oniptothaesntibaelcomferoma acomcmoomnpoupnrdo'csedupraertittoion or distribution coefficient.
Methods
bTyheadmiosdtirifbiuctaitoinoncoofefftihecie3nMtEnovfirFoMnm3e9n25t,al lLoatbo5r05a,torwyasprdoettoecromli.ned bTehleowm.odifications and unique features of this study are described
FdMete39r2m5i,natlioton50a5s, iwtaswasuserdeceiinvetdhewidtihsoturtibuatniyonfucrotehfefripcuiernitfication. Gcaosmpocuhnrdomealtuotgersaphasictharneaelysoivserlofapptihinsg pceoamkpsoun(dFisghuorwes 1)t.hatThtehis csaommpblienedconacreenatroaftitohness.e peaks was used in the determination of
Sgaasmplcehroamnaatloygsriasphw,as apggfoONi rneeledctornon Hceawplteutr-ePadcekteacrtdorM.odelSep5a71r3ations
wceorleumobtcaoinnteadiniunsging10%a C6arftb.owbayx 210/M8 oinn. 600/.D8.0 msetsahinlCehsrsomosstoerebl W-AW.
I18n0jg,ctiaonnd p3o0r0g,'C.o,venresapnedctdievteelcyt.or tTheempegrasatufrleosw wreartee wseats. 4a0t 2m0l0/m,in.
of 2'95% Argon 5% methane mixture.
5
Tjwaros,25-emalchn-coocnttaanionlinsgol9u0t0iomnls ofof deFiMon3i92z5edwewrateera.ddedThetsoe anm-boecrtagnloalss SFMolu3t9i25o.ns HciognhtaiFnMed39215000copnpcment(r0a.t0i1o8n)s waenrde20u0s0edpbpemca(u0s.e036eMa)rloifer attrtaetmipotnss batelodwet0e.r0mi1nMiwnegretheunsduicsctersisbfuutlionsinccoeefofuircieanntalwyittichalcopnrcoe-nrceed-uerxetsrawcetreed nfortomsutfhefiwcaiteenrtlyphasseensiitnitvoeetthoyldetaeccettatteh.e FM 3925
Tshheakejnar%s16usehdourisn tatherodoimstrtiebmupteiroanturceoef(vf2i2cienCt.).detTehreminoacttiaononlweprhease wcaesntrdriafwungeoftfubeasndatnhdecweantterrifpuhgaesde atwas10p,l0a00cedrpmin (21530,-7m0l0 pxolG)ycafrobronate w1a5s mitnruatnessf.errFeodlblyowipnipgetcetnotriafusgeaptairoant,ory600fumnlnelof whteherewaitterwasp,hasien truermno,valdraowfnoocftfanoilntoconatasmeicnoantdiosnepatrraatnosrfyerrfeudnnebly tthoeenpsiupreettitnheg procedure.
006536
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Figure 1.
G1asppCmhsroolmuattioognraomfofFMa35925yl (#i0n8j1e,cti7o/n13/o7f8)a.
Preeapkrseseantt3.F8M3,39245..34, Gaasndc4h.r8o8mamtiongurtaepshic
conditions are given in text.
006537
Co
-a-
Tahned wtahteerethpyhlaseacweatsateextexrtarcatcetd wtahsreeconticmeenstrwaittehd ettohy1l5 amlcetaatter,oom ettehmypleraatcuerteateundwears ndiettreorgmeinn.ed bTyheeFlMect3r9o2n5 ccaopntcuernetragtaisonchrionmatthoegraphy. AThetyopcitcaanlolgasphacsheromfartomogrthaem odfistthreibwuatitoenr pchoaesfeficiisensthowdneteinrmiFniagturieon2. wwaass naostsumaendalytozedbefuonrchFaMng3e9d2.5 cTonhtiesnta.ssumIptstioFnM w3a9s25jucsotnicfeniterdatsiionnce mpreeavsiuoruesmenatttoefmpttshetoFMde3t93e5rmicnoentetnhte dfirsotmritbhuetiooctnancoolefpfhiacsieentfrhoamd sbyhowwnatetrhatexttrhaectFiMon3.925Acoganscencthrraotmiaotnogwraasm nsohtowidnegtectthaebloyctalnoowlerpehdase after extraction is shown in Figure 3.
Results and Discussions F1M000392p5pmcaonndce2n0t0r0atpiopnmsFMin3t9h2e5 wna-toectranpohlasessolsuhtaikoenns wweirteh t0h.e0189 ppm acnodeff0i.0c3i3entpspmofre5s2pe,c0t0i0vealnyd. 60,T6h0i0s. daTthaecoarvreersapgeonddsisttroibduitsitornibution coefficient for FM 3925 in n-octanol/water is 56,800. Bpoansdesd otno tahepremdeitchtoeddofbioNeceolnyc,entBrraatnisoonn &faBcltaoirrbe(t3)w,eenthifsishcormruessc-les asinmdiwlaatrercomopfou5n0d0,. FOMur342l2a,borgaatveoryvalfuiensdinignstohnisthseamestrrauncgteura(l4)l.y TiontdailcabtoeddyaabcicoucmounlcaetinotnratoifonFMfa3c4t2o2r inotfo+5j0u0v.enilUeponchatnrnaenlsfecratftiosh clear water, there was 50% clearance of the bioconcentrated FM 3422 within four days. T(h5e6,8l0o0wervs.dis>t1r00i,b0u0t0i)o,n caonedfftihceiegnrteatfeorr wFaMte3r925solcoumbpialrietdy, tofo FFMM 33402225 + t(r0a.t8e2 mtog/1a vlse.sse0r.05extmegn/tl) th(a5)n, FSMug3g4e22s.t that FM 3925 will bioconcen- ;
References: (1) Burnett, R. Science 174:606, 1971. (2) Gustafson, C. G. Env. Sci. & Tech. 4:814, 1970. (3) N&eeTleyc,h. W.8:1B.1,13,D. 19R7.4.Branson, and G. E. Blair, Env. Sei. (4) WoefltFeMr,542A.2, N.3M ETevcahlnuiactailonRoepfortth,e BAiuogcusotnce1n6,tra1t9i78o.n Potential (5) WTeelcthenri,calA. RNe.p,ortE,. AJ.anRueainrey8r,, S1o97l9u.bility of FM 3925, 34
EAR/cen
006538
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006539
Figure 2. Gas Chromatogram of the water phase from
an n-octanol/water distribution coefficient
un
4f.o3r8,FMa3nd9254.8(4#08m2i,nut7e/s13/a7r8e).takPeneaktso raetpr3e.s81e,nt
FM 3925. Gas chromatographic conditions are
-
,
.
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25
|
:
:
2
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Figure 3.
Gas Chromatogram of the octanol phase from an n-octanol/water distribution coefficient determination for FM 3925 (#163, 6/20/%8).
Tahned paesatker atthi3s.4p3eamkinurteepsresaenndt shFoUul3d9e25r,s Gas before
Chromatographic conditions are given in text.
006540