Document p29NOXNX1Or8LYza9z9DYzeoj

Norwest Biosnlyical ARRQG -- 1212 rSetpuodnyirNoo NNwWBBSR0O0L-O1G28E Quantitative Determination of PFOS and Related Compounds in Human Serum by LC/MS/MS Sample Analysis Report for Protocol EPI-0013 NortAhwDeisvtisBiioonanoaflNytWicTalI(ncN.\WB) 1121East 3900 South Salt Lake City, UT 84124 PREPARED FOR: 3M Company Corporate Occupational Medicine, Medical Department 220-3W-05 St. Paul, MN 55144 2 on = 30 on x OF = AUTHOR: Loins 0Aula Connie O. Sakashita, B.S., NWB Project Manager mo J FOR E. BY: 57 Rodger L. Foltz, Ph.D., N' `Technical Director DATE: /-9:02 DATE: _/-%-12 CONTAIN NO CB! Pagel 650463 Northwes Batya RSepuonryt NNoo. NNWWEBSROO0L0--016256 `QUALITY ASSURANCE STATEMENT LABORATORY: L121 Bast3900 South Northwest Bioanalytical (NWB) A Division of NWT, Inc. Salt Lake City, UT 84124 SPONSOR: C23o0Mr3pCoWro.amt0pe5aOncycupational Medicine, Medical Deparment Paul, MN 55144 COMPOUNDS): NWB STUDYNUMBER: PFOS, PFOA (POAA), PFHS, PFOSA, PFOSAA, M556, M570 NWBS00-128 SPONSOR STUDY NUMBER: ~~ EPI0013 NWB STUDY TITLE: HQuuamntaintaSteivreuDmebtyerLmiOnaMtiSoMnoSf PFOS and Related Compounds in "nsToihndecelclilinininecicasoallfssttutrdueyd.yUdSHe.oswcFerviDbeAerd,GioNnootrdhtihsLwarebesoprtoarBttiosoraynnoaPtlryaitcnitccilaculedcReeodgnwudilutachttiisnoantlshfpordroejfNeiocntinsteiilonniaioccfcaaorLGdaLabnPocreraetagoutrlyhaiSteeed cs Q(L(TaOAibrtUodlrieanp2tae1onrrcCSyeOFPNFRro,Pa.actr2i1tc,e5P8S)At,aBntdhNeaorOtdiEOiCradDtinoPanrnicNnoce.ifpol4re2sNo4o)fncGTliohnoeidcfaoLllalLboaowbriaontrgoartiyonrsPpyreaScctttuiidocineessawnoednretthhpeeeJScaatpfoaerntmeyesodefbMDyHruWgsGAoWoBd Inspection and Reporting Statement. In2s8peNcotvio2n00D0ate PhAnaasleyiocfaSl uPlvan 2005-.2036 MAparr22000011 SSaammppllee ARencaeliypstis 0321-D0e4cM2a0y0120-00171302002 QRCepPorretpDarraatyioRnawData 09 Jan 2002 Final Report A2W8 NBovDPra2o0tj0e0IcntsMpaesctaigoenrRep|3No0rWtNBoIsvMsau2ne0da0gT0eoment 2036 MAparr22000011 3310 Maparr 22000011 0047 MJaany20200201 33113Mamy20200201 09 Jan 2002 31 Jan 2002 "Reports to NWB Management are issuedmonthly. Ahissciannalesrseopnoratblaycsbueraetsetlayblriesfhleecdt, tthheermewthdotd.s an procedures described and th resus incorporated ino SNWhBeAQlAUVaCeomwpsl,i:aAncReSAuditor Bae 02 Page 650464 Norwest Boast RepSorytNNoo.. NNWWBBSR0001-102686 COMPLIANCE STATEMENT `The clinical study described regulated nonclinical study. in this report However, to is not included within the definition ofaGLP the bestofour knowledge, this project was conducted in accordance with the guidelinesofthe U.S. FDA Good Laboratory Practice Regulations for Nonclinical Laboratory Studies (Title 21 CFR Part 58) and according to the methods and procedures described within this report. In addition, the study followed the guidelines ofthe OECD Principles of Good Laboratory Practice and the.Japanese MHW on the Good Laboratory Practice Standard Safety of Drugs (Ordinance No. 21, Ordinance for Nonclinical Laboratory Studies PAB Notification No. 424.) Any known incidents that may have affected the quality or integrity ofthe project or reported data is included in this report. This report represents an accurate recordofthe raw data. Lownie OdebLe Z Connie O. Sakashita, B.S. 'NWB Project Manager Rodgef L. Foltz, Ph.D. NWB Technical Director 19-02 Date [=9--q2 Date 0004.65 Pages Northwest Bioanalytical TABLE OF CONTENTS ReSptourdtyNNoo.. NNWWBBRSOO1D--016268 SIGNATURE PAGE... | QUALITY ASSURANCE STATEMENTwc COMPLIANCE STATEMENT c.ccmssssiissssssssssssssssssnen3 TABLE OF CONTENTS msm LIST OF TABLES... LIST OF FIGURES... sss 1 INTRODUCTIONvss 2 METHODOLOGY cummins) 4. REAS NDDU ISCUL SSIT ON S ccs 3 6. DATA RETENTION ccm 20 LIST OF TABLES `Table 1. Calibration Curve Summary for POS... 2] Table 2. Calibration Curve Summary for PFHS wo... 22 `Table 3. Calibration Curve Summary for PFOA... 23 Table 4. Calibration Curve Summary for PFOSAA wc... 24 Table 5. Calibration Curve Summary for PROSA werner 25 Table 6. Calibration Curve SUMMATYFOr M556... 26. Table 7. Calibration Curve SummaryforM570 vrs 2 Table 8. Back-Calculated ConcentrationsofCalibration Standards for PFOS v.28 Paget 650468 NorthwestBioanalytical ReSptourdty NNoo.. NNWWBBRSO0I0--016268 `Table 9. Back-Caleculated ConcentrationsofCalibration Standards for PEHS .....c........30 Table 10. Back-Calculated Concentrationsof Calibration Standards for PFOA .c..........32 `Table 11. Back-Caleulated ConcentrofaCatliibroatniosn Standards for PFOSAA...............36 Table 12. Back-Calculated Concentrationsof Calibration Standards for PFOSA............39 Table 13. Back-Caleulated Concentrations of Calibration Standards for MSS6 v.......4 Table 14. Back-Calculated Concentrationsof Calibration Standards for M570 ...............43 `Table 15. Analytical QC SUMMA for PFOS vrs `Table 16. Analytical QC SUTMATY fOr FHS... 88 `Table 17. Analytical QC SUMMA fOr PFOA west S| `Table 18. Analytical QC Summary f0r PFOSAA crs Table 19. Analytical QC Summaryfor PFOSA cvs ST `Table 20. Analytical QC SUMMATY fOr M556... 60 Table 21. Analytical QC SUTNELY FOF MS70......rcrssmssssssrssrmnnS2 `Table 22. Study Sample CONGENTBONS c..orrrnsnsinsonnenn 83 Table 23. Repeat Analysis Table 0 PFOS vrs 104 LIST OF FIGURES Figure 1. Representative Calibration Curve for PFOS crc 105 Figure 2. Representative Calibration Curve fOr PFHS ......cccscurnsinrsnnsnn 105 Figure 3. Representative Calibration Curvefor PROA....curnrcrmssrsrcnnnnns 106 Figure 4. Representative Calibration Curve for PROSAA....cccusrssnnrs10e6 Figure S. Representative Calibration Curve for PROSA cums 107 Figure 6. Representative Calibration Curve fOr M536 ...covrssursnscsnsscnsn1n0s7 Figure 7. Representative Calibration Curve fOr MST0 cnr 108 Pages 0504e7 Northwest Bioansiytical ReSptourdtyNNoo.. NNWWBBRSO0I0--016268 Figure 8. Human PLasma BIAnk fo PFOS orton 109 Figure 9. Human Plasma BIank fOr PFHS wv 10 Figure 10. Human Plasma Blank fOr PFOA vrs 1 Figure 11. Human Plasma BIankfor PFOSAA ovens 12 Figure 12. Human Plasma BIank for PFOSA wcrc |13 Figure 13. Human Plasma Blank fOr MSS cv. | 14 Figure 14. Human Plasma Blank for MS70 cscs 15 Figure 15. Human Plasma Blank with Internal Standard (QCO) for PFOS .......c.vcrrr 116 Figure 16. Human Plasma Blank with Internal Standard (QC0) for FHS... 117 Figure 17. Human Plasma Blank with Internal Standard (QC0) 0 PFOA... 118 Figure 18. Human Plasma Blank with Internal Standard (QC0) for PFOSAA. corn 119 Figure 19. Human Plasma Blank with Internal Standard (QC0) for PFOSA rn 120 Figure 20. Human Plasma Blank with Internal Standard (QO) or MSS... 121 Figure 21. Human Plasma Blank with Internal Standard (QO) for MS70 evo 122 Figure 22. Low Standardfor PROS. 123 Figure 23. Low Standard fOr PHS... 124 Figure 24. Low Standard for PFOA wcervssnv mss sss 125 Figure 25. Low Standardfor PROSAA wrest 126 Figure 26. Low Standard for PFOSA..cvvvvovravsansssnstisssnssnmnsssnns 27 Figure 27. Low SIandard Or MSG. 128 Figure 28. Low Standard orM570. 129 Figure 29. High Standard or PROS. 130 Figure 30. High Standard for PFHScvs 31 Figure 31. High Standard for PFOA wrens 32 Pages 5048 Northwest Bioanalytical ReSptourdtyNNoo.. NNWWBBRSOOI0--102886 Figure 32. High Standard for PROSAA vores33 Figure33. High Standard forPROSA ccna 138 Figure 34. High Standard fOr MSS. 35 Figure 35. High Standard fOr MST0.ccrrssmsmnmsnmsmssnssnssnssnsnonns 136 650569 Page? Northwest Bioanalytical ReSptourdtyNNoo.. NNWWEBRSO0L0--016268 Quantitative Determination of PFOS and Related Compounds in Human Serum by LC/MS/MS Sample Analysis for Protocol EPI-0013 1 INTRODUCTION `This report summarizes the analytical results from the quantitation of PFOS, PFHS, PFOA, PFOSAA, PFOSA, M556, and M570 in human serum samples for 3M Company in support of Protocol EPI-0013 [5.1]. The LC/MS/MS method for the analytes exhibited 2 quadratic response and had target LLOQ and ULOQ valuesof1.00 ppb (2.50 ppb for M556) and 500 ppb for the analytes. The testing facility was 3M Company (Corporate Occupational Medicine, Medical Department, 220-3W-05, St. Paul, MN. 55144), and the Study Director was Jeffrey H. Mandel, M.D. The Study Contacts at 3M Company were Jean Burris, M.P.H, RN., and James Lundberg, Ph.D. The following isalist ofNWB supervisory personnel involved in the completionofthis work: Connie O. Sakashita, B.S. (NWB Project Manager); Tonya Felix, M.A. (NWB Acting Project Manager); Patrick Bennett, M.S., MBA. (NWB Laboratory Director); Rodger L. Foltz, Ph.D. (NWB Technical Director). Laboratory personnel involved in the completionofthis study included Suzanne Newman, B.S. (NWB Research Scientist), Toni Peacock, B.S. (NWB Assistant Scientist) and Emily Yardimei, B.S. (NWB Associate Research Scientist). INWB SOPs and guidelines wereused in the conductofthis study and were available to study personnel in electronic and hard copy formats. Date Study Initiated: __15-Nov-2000_ Date Analyses Completed: _ 11-May-2001 `The clinical study described in this report is not included within the definition ofaGLP regulated nonclinical study. However, Northwest Bioanalytical conducts all studies within the guidelinesofthe U.S. FDA Good Laboratory Practice Regulations for Nonclinical Laboratory Studies (Title 21 CFR Part 58), the OECD PrinciplesofGood Pages 650470 Northwest Bioanalytical ReSptourdtyNNoo.. NNWWBBSROO0I0--016263 Laboratory Practice and the Japanese MHW Good Laboratory Practice Standard Ordinance for Nonclinical Laboratory Studies on the SafetyofDrugs (Ordinance No. 21, PAB Notification No. 424). Any changes to or deviations from the original protocol (Analytical Plan) were documented through approved protocol amendments or deviation memos and are retained within the raw data. 2. METHODOLOGY `The assay used for this study is reported in Northwest Bioanalytical reports NWBROO108 [5.2] and NWBROO-122 [5.3]. Chinese humanplasmawas used for the calibration curves and quality control samples. Serum sample results obtained using plasma curves are discussed in NWBROO-122. `Samples for this study were received at NWB on the following dates: Receipt Date, 22-Nov-2000 21-Feb-2001 06-Mar-2001 15-Mar-2001 05-Apr-2001 NumberofSamples Received 100 108 330 16 125 Storage Condition (except during analysis) 20C 20C 20C 20C 20C Reference Material e Analyte Lot Nue me ber e Purite y Ex-- piration -- e Source -- Storage C-- onditions Date PFOS (FC-95) 193 100% 31-Dec-2010 3M, Room temperature PFHS SE036 100% 01-Jan-2010 3M 20C PFOA (FC-143) 25 100% 31-Dec-2010 3M Room temperature PFOSAA (FC-129) 617 538% 31-Dec2010 3M Room temperature PFOSA 214 100% 31-Dec2010 3M Room temperature M536 NBI13047-8D 99.89% 31-Dec-2010 3M Room temperature M570 118506-26 99.75% 31-Dec-2010 3M Room temperature THPFOS 59909 90.0% 31-Dec-2010 3M Room temperature "Stored dey Pages 050471 Northwest Bioanalytical ReSptourdtyNNoo.. NNWWBBRSO0I0--016268 Eight or more calibration standards were prepared on the dayof each run by adding 100 uL of blank human plasma and 400 kL of 50 mM ammonium acetate in water (unadjusted pH ~6.9) to 13 x 100 mm polypropylene tubes. After a briefvortex mixing, 10.0 of the appropriate spiking solution was added. `The absolute purity ofPFOS, PFOSA, PFOA, and PFHS reference material was not available prior to the conductofthis study. Therefore, the reference material purity was assumed to be 100%. 3M contracted with Centre Analytical Laboratories, Inc. in State College, Pennsylvania to determine the absolute concentration of PFOS, PFHS, and PFOA in NWB stock solutions used to prepare the analytical standards and controls used for this studies. All arrangements for purity determinations and the transfer of NWB. solutions to Centre Analytical Laboratories, Inc. were performed by 3M. Based on Centre Analytical Laboratories' results, the concentrationsofplasma calibrator and `quality control samples were corrected using the following factors: Analyte Correction Factor PROS 0836 PHS 0909 PFOA 0855 `The final calibration standard concentrations in human plasma for each analyte ean be found in Tables 8 ~ 14. NOTE: Due to the use ofdifferent matrix pools with different persistent levels, thereareseveral standard concentrations in these tables. However, only eight (for M556) or nine (all other analytes) standards are usedfor a single curve. The target calibration curve range is 1.00 ppb (2.50 ppb MSS6) to 500 ppb prior to correction for purity. In addition, blank plasma samples, both with and without intemal standard (designated as QCO0s and Blanks, respectively), were assayed in each analytical run. Analytical QCs were prepared in human plasma on October 27, 2000, March 8, 2001, April 5, 2001 and May 2, 2001 and frozen in a -20 C freezer. For each run, analytical QC levels were assayed in duplicate. In addition, for each dilution level, dilution QCs posed 050472 Northwest Bioanalytical ReSptourdtyNNoo., NNWWBBRSOOI0--016268 were run in triplicate in any analytical run that contained diluted subject samples. The QC concentrations are shown below: e--e-- ee---------------------- QC Concentrations* Analyte --A--l-- lcAoll conncentcratie onssrrn eeeexxppt rreessssr eedd asstppppb..ions Low Medium High Dilution epP--FO--Seee ee57e5,6e40eemm126mgmr31r,33g2 eeDeD3EF4=e3s e PFHS 4.48 156,157 417,418 PFOA 432,481 14s 385 PFOSAA 430,460 150, 151 400,401 PFOSA 4.00 150 400 Mss6 4.00 150 400 M54710SR...4.00SUL..1.50NU...400A E*aTchhestnaarlgyetteQhCascdoinfcfeenretnrtatQioCnscoanrcee4n.t0ra0tpipobn,s b1a5s0epdpuspoanndth4e00peprspibstpernitorleovelcsororftechtioancafloyrtpeurfintyt.he human plasma used. `The internal standard (THPFOS) was added to all plasma and serum samples (except Blanks) for a final concentrationofapproximately 200 ppb. `The analytical method consisted ofa liquid-liquid extraction procedure followed by `evaporation and reconstitution of the extract residue with 20 mM ammonium acetate in water: 20 mM ammonium acetate in methanol (30:70, v/v). The samples were analyzed by liquid chromatographytandem mass spectrometry using an API 3000. The instrument was operated in the multiple reaction monitoring (MRM) mode under optimized conditions for PFOS, PFHS, PFOA, PFOSAA, PFOSA, MSS6 and M70 detectionofthe negative fons formed by TurbolonSprayTM ionization. 3. SAMPLE ANALYSIS PFOS, PFHS, PFOA, PFOSAA, PFOSA, MSS6, M570, and THPFOS chromatographic peaks were integrated using the MacQuan software (version 1.6) with a smooth factor of one. Quantitation was based upon quadratic regression analysis of weighted (1/x) calibration curves using area ratio vs. concentration utilizing Watson DMLIMS software (version 6.1.1.04). The samples were injected in a systematic order. Page 11 050473 Northwest Bioanalytical ReSptourdtyNNoo.. NNWWBBRSO0I0--016263 For sample 11689, the measured concentration of PFOS was corrected for the persistent level of analyte present in the dilution matrix. The concentration is reported as a whole number and has not been rounded to 3 significant figures. 31. Acceptance Criteria For an analytical run to be accepted, it must have met the acceptance criteria listed below which are consistent with regulatory and industry recommendations. Calibration Curve Each run will include calibration standards in duplicate at six or more concentrations covering the lower to upper limitof quantitation.For all analytes except PFOSA and PFOSAA, at least three-fourthsofthe calibration standard's back-calculated concentrations must be within + 15% (+ 20% for LLOQ) of their individual target concentrations. For PFOSA and PFOSAA, at least three-fourthsofthe calibration standards back-calculated concentrations must be within 20% (+ 25% for LLOQ) oftheir individual target concentrations. A calibration standard will be considered an outlier ifit is greater than two times the acceptance criteria for that standard. Lower Limitof Quantitation The back-calculated concentrationsofat least one of the duplicate lowest points in the calibration curve must be within + 25%of the target concentration for PFOSA and PFOSAA to qualify as the LLOQ and within 20%ofthe target concentration to `qualifyas the LLOQ for all other analytes.Ifthis criterion is not met, the next level is subjected to the same test and the LLOQ raised accordingly. Quality Control Samples Each analytical run will include low, medium and high QC samples in duplicate. The measured concentrationsofat least two-thirdsofall analytical QCs mustbe within 20%oftheir target concentrations (+ 25% for PFOSA and PFOSAA), and no two QCs at the same concentration can be outside the limit. Ifstudy samples require Page 12 050474 dilution, a dilution QC will be analyzed in triplicate for each dilution level (except for PFOSA and PFOSAA which should not be diluted with control matrix). At least two dilution QCs at each level must be within + 20%of its target concentrations in order to accept diluted study samples at that level. The dilution QC acceptance is independentof the undiluted analytical QC acceptance. 4. RESULTS AND DISCUSSION Serum sample results obtained using plasma curves for PFHS, PFOA, PFOSAA, PFOSA, MsS6, and M570 did not meet NWB SOP requirements for validation. Results may vary on average up to 26% from results obtained using human serum calibration curves at some concentration levels for PFHS, PFOA, PFOSAA, M556, and M570. For PFOSA, results may vary on average up to 43% from results obtained using human serum calibration curves. Thesedataare reported in Assay Revalidation Addendum Report NWBROO-122 (5.3). However, inorderto obtain the lower limitof quantitationofless than 5 ppb required by the sponsor, it was necessary to use plasma calibration curves. `Each accepted run met the acceptance criteria set for the calibration curve points, for the Tower limitof quantitation (LLOQ) and for the analytical quality control (QC) samples. [Re] ore [ge wee woeJolin] comm] [[[T1oF]rF Porons||Acccepptr ed[|[27-zNov-t2000[[|[o [2so7:vNoivt-2s00[0[||oaos a0[[|sasnms| | |t ] ]| E[[NvossenPnoppee [E oervsayt [[raemmesa)[306[|AoiTmo| |] ] [EC 2N Fvrome Eneepse [[Estonyzo[[sseoiszoy[| o0n[[Aaorm| |] | [[2T[FFoonnceps[| evrasn[nvivsm[|v00[sao||] (3Tvs nope |eames om |] 650455 [[[Tio[5Wr605e0|prrsepgpe|[]osvtporn[|vvriaoinnei|3a00i[|5n0]| | o] me] 1E I IE ay Plo]TTT oem] 1I I outage (repeated in run 6) PIT TT fT ermen] outage (repeated in run 6) CllT TT Tee es e 1I IE I I I eo I ToPrrotsnE pps|oavwwznon[|vmEivriNor E 1a0r[0| E a] e ] ([C[CoTT[rFroomnso[ncsceppset ssevaaatsiooens||ssaviaannr|[T1i103[6assorr| | |] ] ] [[[5C5T1ovevroes [|sscmeoopsessJJsaavwwaatnn[|ssmvaaireriicooorn [|33o0n0][| setoo |JJ--oo--rc]]] [[[C55o [rrerroootsnaa|naaoccpeeesssswviizmon[[|vssivvctaaorrr|viro0|a0 sonr| | ] ] [[[55T5vv8it ecoppeees |[sssvvtssotor ssseevnwoinrT[|| 1r0[a0| |] ] ] ([[CC7oeT[re#ro7o0ms5aan||anccepps[|ssseovvaarsiror[szeeovvveteronn|[|a1223100 Tsa0oer| | || ] ] [(Coo1T8Pr8on[acepes|sseevviarisoonr[sevoismioor[3a0|sam| | ] ro 050476 Fe ld cy [[C7e[T5oe0ro5os||aawcpeeperess[oseovvacvttioionne||ssseievawmon|30r3|saone||| [[[7er[rroroonssan[a|aacppcetytT||ssseevetvvaaiiiooonnn[|[avssoivvsiiwcimaooorrTarna |||as0o||] [[[775[[o7ss0ss|||amaccppepetet|sseeemsvcsatoon[||szsoevwwaronrr[|23| 0 |s3o3r||| [[[51Css[TTrerrroooosasnn|aacpeppeeet|stssvrstaorainonna| ssvsaaasioor[[v3io [ssamrr||| [[[s15so[Teroresosn amapceegpeeettsssanvasarsre[|zsssavmicaorornrT23a00|s5aor||| [[[C5oo TTrsoorscseT||aaAccpeppeeest|assssssnor||a ssmaacat ronr[|o rv7oino[|sam 3oe0r| |]is] t [C[[oeecJ rocvons[|acaceaggpeeestt||sssesvsvcsiotor |ssovvraaiorrn[2222000 |3ase| || | [[CCo[o[wvrsoms|rRaoecmpeestt{[sastocvaittcosoosr ||ssvvosawsrn[[|[ 51a20 ||sonra JJomomemmic] eaer ]| [[[oCoTTTeeFrerooos5nane|TaaaeccepepesetT [s sovaioo no [sovem iv or[r1at i |||assi0| | |o e --] ] --m t| [[ooTrssss [om || raccoppetr Ssoovsraon ssoovmmeoorn cet[sovaciorsin 2320 110 |s3o5r| || ] 20 |] C[[oTTTororroooossn| saceceeppetr[ossovvviirassorr nssooimmeenoonn[|3a14300ssarmrT| |eme] s] Pegs ts 650477 ET [feo] I[EeNiEsE nA pEsEoT wssEvwearny|I2E00[ ET C0 o ] ([svem|pcess ssovoarssoor[[ssoowwwaszoonnr||31290| ssoorr|Jomo] | | e EE e se [[5i[rorooesn||csets||cohrotiorn[|emepzomn|Tav3[[a0| | | [[[0Toe1riroossn|opeccmeesr|||scoraoovwon|[oeoprvemazeonr[aa2n0|0[|oam | ||--| ] [[vvsme |snecnse||aoween[[oommrpeermon |[ 200|[soro[|waives [[[tFseoro7r0oos5n]||ancccpees |||oscxssoooczon[[orommmegaserroionn[[41237[oo0rm| ||] | [[[Cieisroorens |||mnaecpss|||soxssooertsoon[[raoarmzsaoonnn[|33a20n00|5osos| | || ] ] [[i[reroos||raecpess||sstsoow [omreason|| 1r[aoer|| [[iroorsose | anccees ||bsousetson[|oommmggeon[|1a00|]| 0|] | [[irTriosn || spccs|sbwsewzon[[moemaannT[2a0r0[[3a0| || ] [[i rvios |pcppes||acsosretrit [[eovmagpo[|13900 ooor| |] ] [[iteTreorsose||maceess|| oroertoon[| veprann|a100[ai0| |] ] [Toros mes| sors | gon| |srJems] 650478 Lm Fd i ([[Tiee|ioersioens||snmesss[|[ooorrvagteaonnr[|vgisapgeomrrm[||a332o70||[oomr|J[eweesm] [EiNTCvEs|EsysTeomayto rErEp I 0|[omens] [[[0777 rrroorsosan[|anspecesps|||ttoerosiwtonon|||maesesroaonn[|1a01[|oa0me| [ || | ] [[[777vriisms||anseoeepsms[|tooomsogoemin[[| gmsaeprnmo[i32s0o [ooonmr| | |] ] ] [[ii r3r7o0s5n |eaecess || [Feros ses| wietonr[|tmeomaon|1 [a50 | || ] orzo | pean[0 0|] [[ii]|rrrion|nacses|| eectto[|vmpacn[|2a0v oam| | | [[iovisse|mnsess| oerczoto[| nppeann|31200 | o1r| [eme] sis| [2[ros[itors Tit| | fogs li) dill === [oreo | pet| itor|ern|5|7[s[scretoemtrc | [C[ormsrson[|mrosinesss[|[o6oawoperonrm| TeasrmmgoiaTro[|510[|5s0rr[Trnreomieoetnrei,se || [[oTissero ||arcepsso|Greotmon||wearagrraon||3133||sotrJJmrsaimimnostiooamttss || C ET ET E EE i RT and QCs) _ 050479 nt wr| ir es oem am | oo rowenta | mT vir ee | rm om | ToT eer [om foe -- and QCs) ART e y Cee e pen e re t er Tys E and QCs) i Ee r es e SET Seon rer [ro vse |7 foser [Ea ofnewlow QC vr ra enerin [5[Foonms[ion|ovo| 15 | 1 [spp | it 050580 E[moT efeof Sn [[[555T[Feo0eonn||cssecssp[[[iotntvoroans[[oawmvnorar|| a5r8[||so| |sap] ere| [[5To5mLpets[ [t00v [amr or |1o6na [|sam | oi e] ms] In run 4 for PFOSA and PFOSAA, one of the medium QC samples gave extremelyhigh results. These results appear to skew the overall statistics for these analytes. Without this "outlier", the overall %CV was 9.2% for PFOSA and 12.3% for PEOSAA. The.overall %Bias was 2.0% for PFOSA and 5.3% for PFOSAA. In run 8 for PFOA, one of the low QC samples gave an extremely low result of 0.161 ppb. This result appears to skew the overall statistics for the analyte. Without this "outlier", the overall %CV was 14.1% and the %Bias was ~10.0%/ Any known circumstances that may have affected the quality or integrityofthe data are included in this report. 5. REFERENCES 5.1 3M Company Protocol EPI-0013. "Identificationof Fluorochemicals in Sera of American Red Cross Blood Donors in the United States." September 29, 2000. 52. C. Sakashita. "Quantitative Determination of PFOS, PFOSA, PFOSAA, POAA, PFHS, M556 and M570 in Human Serum by LC/MS/MS." Assay Revalidation Report. NWB Study NWBS00-040. NWB Report NWBRO0-108. January 24, 2001 5.3. C.Sakashita. "Quantitative Determination of PFOS, PFOSA, PFOSAA, POAA, PFHS, M556 and M570 in Human Serum by LC/MS/MS." Assay Revalidation `Addendum Report. NWB Study NWBS00-040. NWB Report NWBR00-122. November 20, 2001. Page 19 050461 Northwest Bioanalytical ReSptourdtyNNoo.. NNWWBBRSO0I0--016268 6. DATA RETENTION The raw data and final report for this study will be stored in the NWB Archives, 1121 East 3900 South, Salt Lake City, UT 84124 per regulations and contract agreement. After submissionofthe final report to the Sponsor, remaining study samples will be stored under required conditions until confirmationofSample Disposition/Returm Authorization is received from the Sponsor. 3M Company will be notified concerning final disposition ofrecords at completionofcontract obligations. Page20 050482 Table I. Calibration Curve Summary for PFOS. Quadratic weighted 1/x'. All concentrations are expressed as ppb. Cl [[Ceoovsnreton||| +=Tosoo oo_ n Tm oommsoso|||woom_ oar ormnns|||rooowvwaesso r | |||s3w 5ossu ||| assvie||] [eevossetitoorr||--5|-aToaormoomn[[oonmions|| oooomm ||"oowss ||ssoru]| oaii|] [D(oovesasetrsateonr||[51+0 Tooossaooiononn[|[ooooamrorewsnn ||`oaomoaarrnmsn|||ooovwsessr |||essaazrr ||| oaariu||| [o2ovimcator||1 15 [orton| 14 soowtoowor[[ooowms somo [wows ||oasmsmees||oowsns|aazz |sors |ows | ss || asiie|| ois| [[tsocraotor|[1is [cor[17 soosowoomnonsr[[ooossrosn|||ooosmsome|||ooowwsnss |||eeaazzr]|| oasrire||| e C-- esapesoor| is Taoromn[onion "omer |oosss|ar |sv| h eT C osol] TToomormsT[[coowmsmT sT[[vmwoemT[|oowseo I| |T] |] JS 050483 ane psn Table 2. Calibration Curve Summary for PFHS Quadratic weighted /x'. All concentrations are expressed as ppb. EENEI E Number [evs | Eosoems [aosiorsy|Oaosrszs|oCsEma|E 1a3l|E soe] [ovseior|| osowte one|somes | ass | 36 | ar] Ceovacisor| ouowns [somim |-osoes |"os | 505 | rr] [CCEeossssveerreionr|||i|ooosstooowwmonorer[[oooouwmsvsor||| waooomrmameasse|||oaamssma]]720000|||s1sri]s]] [Coevvseetiorr|| 15 ooowommor |[aasomn Corsortoor |e oiomos[ont |osroaor ||"aaosmiis||7705| ssoir]] |sore|"ans | 205 | 1] I [mo |T I i ouT omor[orT TsY|IomCaErT |CaEswTs |T 00|] [s=ol oTomT[NoCom oes| somo ||] I mt rTeA iT n ammT o T - 650484 Table 3. Calibration Curve Summary for PFOA Quadratic weighted 1/x'. All concentrationsare expressaesd ppb. El [[svForraattmo|||[oouwnn [sama | Tomo |swoorree |oom ||asooeso | soome ||"a|or | ova o3v3 153 ||a || | at] [[eEasuvwaarsanmsn | | e oooumoowmnor|[sooorrmo[|asomwmosrr |"amasw i153 ||aa || [[[eoosvosvsteinwnasatttnoesr|||iv o |swooorootmmoo T||ooommmmeairre|T|oasmooearrsse[||soaoosmusess|||2i1||asar]|] [[eerexcititon ||5 1c Cotacnor[is oosuooiwnosr |[swoomws soows Toms ||osoomors[|aaomsmre |ai]| aa]| [oars |orn |zis] [[seimtaor||ri|owwoorwoomro[|wsoomees || ssooomoass ||aasmsess ]| || awrn|| e [[a-- issonanosn||| oowsoowwoorr|soorerse||asmoewss[|a"essspe|ars||aor]] l C [swoe ] TTTvuoevmorms[vowneeo si||ovnomssT| sooo sns|| T|] ]| _-- G56485 eres min -- Table 4. Calibration Curve Summary for PFOSAA a Quadratic weighted Ix', All concentrations are expressed as ppb. [Fersvooaraomn||_Nu1mber oosooowsn [svtaioor| Tour owoomaroes||ooooowsr ooo |aoomes || | assommo ooass || rrwa || 1]| |10 51| [[esosvereamn[|o+oTorwn[oomvoownmo|| ooawmsesss||aossse||r25a0 |ssri|] [Eo[CvTeinasEoanwotn[|5 1E0N EootCvoommoosrT|ovoorsntyse||RoTassoomusas||RaooeRsm ||E32Ts30]|sR5Er1|| [[soriartasoos|| 15 Joossoomwnoer[oowoems||oooorrmaesr||aassessess||azs0w| s]or] [[erzarmitoonr|| [amon| oin> is Toosoone[oovaoenoo||odmaamre|| aossms ||2210 ||swoor]] Tomoms[owomos|acon| srr|230 2] [r sSoellTTTooumooT wmosr[[voowrT ess||owoomT ||ooween1 s | || ] Cedl TTwvol [o2[umos l| oor TlT] A, B, and C are coefficients used to define the calibration curve. ET Bde Table 5. Calibration Curve Summary for PFOSA i I i li Quadratic weighted 1/x'. All concentrations are. `expressaesd ppb. [[veosooatro[ | Num2ber| oossoomas[avvowess|[wvoomrosnarr||awomm ||uosor||wsoo]] [r[Csestsna [| 5]To-wouoowmosr[| ovooor|| o0m0nmim||aasmms || uosoo|| ssoo]] [Cooesvacwiattoorr| || | oowootmonute [aasosnure|| osoomems || ammsre || 1a30|| o0']] [[aCvesvstiwwzaoonn[ [o5J woomeono|[ooomzamrss||snoorwsis||aamsems ||sussoo|| 00]] [[Csovoinsatooy||t|o oocwowoors|aouoamwoore||owpoursms||aassmm ||1io|| swoo|] [[eexrapteen| |v | otuoowmo:s[[aoiousrs||wooosrrme||aaommiss ||1ve0o || s0o ]| [[iCvoavsorcsitoorr|| ies ossoomoone |oavsanst||oaoo0 ||aaomns||1oo|| osoo|] [[aizsaotriioonr|| 17]oootmomo|[oaorwns|[soonmeso||assomma |]voeno || os'o]] -- TT TT Ceo l TTowwTTseTwmw Tose ||] ] 050487 Rig Table 6. Calibration Curve Summary for M556 Quadratic weighted 1/x". All concentrations are expressed as ppb. Run A c R-Squared| LLOQ| ULOQ Covvovzom[2 otoons[own|aonse | asm |20|sw | EE I srs [[esssirzoonv||0 irzToomotoomontr|o[voomwneess|ososms|ams |520 |so | [[osveesattoosn|| ootmoommosr[oooomomesss||wooomuses||aosomm ||532200|| ssoo || [[zsisnsataos|| 15 oottoowmoons[oovooouotmss||oormeens||aamne||332200 ||ssoo || iors os |5m|or| [sie olTomToowmooms[[sooowss|| wsoooom||oaomor|||||| el TeTw[ew [os [| : A, B,and Carecoefficients usedtodefine the calibration curve. re 050488 Ss es "Table 7. Calibration Curve Summary for M570 Quadratic weighted 1/x". All `concentarreaetxpireosnsesd as ppb. Run A c ReSquared | LLOQ| ULOQ [[[eeaersvoeivazzooomnm|[|5 N_2umbe|r |oooovnoows[[ooovswomweas||| oawvooamaest|||aaomommroa |||"11vi56o]|5s0o7]]] [[[aass2vvieaswizootonr[||75|oowooorotosm [|oooooormeos|||oaooewmrnms||| oaaosssoom|]v1ua3ss0o| soir||] orseraon[2 soon [oon|ooomss | aor |v0|r| [[nEieessErraooTn[|Is5 tEKooOoomoow[TEoomwoysnn||Iaamcmoarn||MooovmTro ||E"vR5RE11|] [etsy [ss ooocwsoorws[[ooswsas||aoavne||ooswssns||1im0||r07|] vel vowon [owe| sown |ssw |] we TTe wT[mws[Twwsi|wT meer rete 650489 AHHHHHHHH] i EIEEEEEBEEEREEEEEEEEE i ENEEDEBEEEBEEEEEEEEE 31 [2lae[ala[e[=]ala[sz[zaz]az[2]5z] Lo ET EEEET EEEEEE [2[ale[e[elTeTTlTeTlTaTle]l 3 i ETT sREEREREREE g = 4 s FBERERTRTETREL 3ELE gz ELE=TET IaREELRELERLR I V SEE TE TLR E rie FSFExIF F |eSEeERe A aid Zi 4 : 30[[ET EET ToT le elT elefT ele] leefe i 5 i EIRENE HEERERARE 852s [= [BeEE[BEs[EeEeeEl[EeeEeeEe[EE8e]N[lzE[E2e[Eol]E3]a4) i fa[elaa a aass ]EEe lRlle] 23312 [2[Ele[e[saelle aalalaz] [[a[za[=l]a]es] 2 53 Eo [FaR[ETEETETE=I=EIRTEIaIE=TlEIzETEeETTRNeE3eEEeEI=]R 8 =8 ,[3[s[= asI- ss|e[elsse[leael[[e[zaallelzs]=) |5 | Eee le 3 ELIRT ll 3383R3ENE g E3 EEREE< T ERET EET Em RNTEel ETeR=fEe EE3: 3522 == 5 - "l= 2 3 oSO ip ek% g2 a Nid 2 3 [2 e[e[e[ale[elz]e[a[ala]o[ale][2lg]=[s]<]5 E iFa x L [1T ] [elas] i TS |3 2 43 3% 33 3 : 3i3g ELLIOTT][efeefel) 2 z| |= = 1i i i gl |B IB] 1B] |B] IB 3 LEHRER EEE 3 8 s 3 = a EY [a E[REEEREEEEEREER ENEEEEBBE REEREBEEEEE 22 Bo [8 [elle aR ell ET eeesezalely] 2 e [Eells ITI] $f LLLeee $i ]L [2 REE aElTeD ell1e[eleTleTe] eTefel T:3 Be S 5 FUEL [SEE E E ee RI TTIE eee i5f sd 5 2 EERE OE 33 ELeL el ] a 2 [efelelef[eLlTsTTlTmTe vo EEE : 8 Je BNENERE sy [i FERRFE E i E30 EE EL 22 [a[2[a]a[e[e]a]z[e[salz[e[se] [a[z[s]2]z] 3 iy ENEEEEHEEEEEEEEENEERER IE: ELT Teale] g [8[alasafsllaes[olae]l[z3l2e] Eels [TLL alee y 1 E1T[e 5[a[s[aeleeale[slald elelele][al2fe[ale)d bE1 E [afeleeleleiefae feleleEleglellee] 3 1EL% eplalslele eeeeelRe] se 3: SERS 3[a[alee pee][af 2= ETH Tele= :pF F RL EEE EE E T i HOE IEEE EG i & | | & &| F1 Zz a2 8 s 3 = 9 ]: i22 Xz i FE s|g)2 3s 3 < [3[ofslsle[sfelele] [eHAf3]4 PN EEEERREENEEERE fLH iHIECEEE5R/3)R8 E iii! 13 FERRJel E ff $113 [AERAEeL]JEaefLaleEl] HEE fs = EF] = 2$14318 EERE 13 |.: EEL] 2 ii = 2HEH|Z 2 8 & ::3 : i 22 8i2 1$s if 5 :3 ag i3 5g egl|=%z(l8a/8|5o g o S$ [[2lalslals[s[eele[ale2[(a8[(38|[%3([3s(]3s] 3[22Pa [aFaElIlaallpealplyleaaepr]llalsaaae[lf[aaa leseal][s3a]esa]ss]s] { 3$1i[d2 llsMU eae EIRIEIEE aleeHEa Ba aElRee] i {1[8 FERER la FE alafR el] 351 [[2F[Rz[Ez]zE]sRfzfs E3 RE] 5 8. zz 2 s|alsla(gle "fem ~ 2fs[s[a[3[selee meee] 5 2 Ej x 2 Jefe eel] 3 olsls i i a $ Ea 2 8 i i gz 2(s(8 8 2(3|%|z(g]= =2 g3 3 iiid 3 o1 i5ol g pd 2 H5 H[5 BJz EJz gl [5] [3] |& : 2 2 ggz 22 3 ii i35 [5 [eles Tefle[? ] 2: S3$ [5[aelalefa[s] [sles]: g [2[s3[|s8[|s5[|e2]e8[a] [l#|z2]]3s]3[]=]| $S3E: |25 Is5llzlge o=l= 2 i a315[Eleaf Ta- fel] F5E:RERLIEBEHE8 3i i3 gi1 [2 aleeala] eekofleifndelLake) s 3 = zl5la = |e -3 & Zsx 2[feefell] [sell 8i ; igz: | Hgl E -glsl2| 1 SZ i 3E 3 3Ie ? 3 5 z 2 El |B * 18 | .i =8 = a B49 2 Z Z 5 a2 H zz 2 255523 23 i% [5Jefeg Teels[]s] SHR EIOENEEE0E 2 [7[EE][EEE] 3b al le 2fs7 2 5 5 [2[ols] [e[ele[s[-| 23 [ETE B i 28] 3 als 2 $1S$ 3 RlEfefelel-] * ai 5[2 ele] fale] 4 ) $f13z1 [3[ef][zlafefs]| ii Ii 8 3:$ E : i s 3 I{ 3 2 Z 3 53 23 22 3 2 [[Els[s[s[elefele]Jefe] 8To [2Lela lefela]Jalal] $i:e[a[elefsfelslelefa] [eEeas] : 315 i[= LHEele2 ]5 5[ofsle] i }1 [5[elEflfalsfafee]Jaa8lsa] 5if 2f3<=1[E2 Ra2lEae agllleeelaela]TJeafall] iif 33 =: E3x[2T|eol[=e[aslaalls[eafsele][algal] 3if 3i1s[[3 elHEHEe=HEelle eJefea5 leole~] i3 ; ; 3=; : i| g3l [3g i iz 7 ? 5 3 EEE EHH] Eo eleelle Bi O[FLR[EELEEOERETREETRREIRER]RR a 1g [LEER 2 2 EOI : EIEBBEBREBEBEEEEERER gE LITT YE EEEEEEEEEEEEEEEERET i ET iq HEE EEEEEEEEBEEERERE] ga LLLITT 2 | [EE erER EE RE RRR] 4 5 seBTTILIITIITI [3 4 $ [[2 a5[eeafelee eefcaaEfezs] od EE TTC [2 Fleas] e efelels eo eles 3] 3 i 3 [als S = ETI : i [alle[[lBReILsL 2 [T[T ss] [s[T efa5]] 8 [elelelelelels][Tefafz[a[A] 2 z or TT Js] [T elefelel] 3S$ [x[e[e[e[TaTTlelaall=leels]] 2 A CRRRNERRREERREE 5 o[2 5(2 [EeTl TTel feles el] LTTE] [alee] il 3EEE][Eel HH ARNRNNNEEREERERE i: pL HHEEHRNNEEEEEE; 2 HI NTETETETTEREeENaNElENE)E ; 3Pe1a[2[JTeTl[eTlse[el1]ee[slfeafe[aefle=][a++]]]25 90: alalal= 2] [sls ft ell[eel] 25 ET TEEET EE] pd BFE] 0 3 Z gE | aR 3 3 AZ i FE 3 ELii: E RaRllia o CEFAASR ig 5So [J2LLoLe]lEllslaaelelaa 83$e[[2TFT= RTE ElelER e TTe T]= i: 2 ERR |[TTT] i SHE TT oleae T eeofa I LE BRTE TT E 3 2[8 L - -T-- eee 1 53 if 3} [EEE TTT $0 [111] Ee] sx FeelTT YH EHH HAE] OEE i & [8 2 5iz [2 EERE EER 33 HIi Y [aCREe[BeEl:slBelEeEllRaHlEEzRaElEEsE]ENNEEREEETeal EEER zi iEo [2le3l1e3lle8l/sl]ezl]aslalelelelae]ENEE[aElEseR] a3 [32 [|e lalEle a RlSESle a JR2 E ~ale : . 54 EINEM 8 1ME[2ME[a2H[RIE2[RR[a[Eafsleel=BEBE 5E]E[E3aN[E2E[zE]E)EiE 5Fi0[2 2E Jefe ee T ea e e I Teell] eefe i SEE TTTTTTTT ofalelL el]3 3= gg 2; z alalz[s]z]s]=[a]]e]s slz| i5 sla [TTTle] 33 IF "[F) i 2 ETOH e3 R i1 eg = 2 2 Sl Hi EE IE IE JE 42 EEE | IE " gl Is] [8] |=] [2] |] 3 [LLL Fe gis [ELEEEERERRERRR 2 [EERE] 3- $0 ELL Tee[eEERRERERE -- &3 *No REBEL]LTT [ELLE ee] CR EER NNNEE 3 [ELL safe] o gE3 28 EEEHET3 TT]i 2E g LT EETRolE=lonEtRelRzREE iEE Sis slelelels i38 5f : 1 i Js 2 ole = =[ Tal3Le T == T=z] &i:f 3 = =lsl=ls]ale 3 EEE i 33 FL E 3s L g 23[F5]8|3/8(3)8] & [2 fetal TTT TTT 2 i2 g3l 133]15115] 3]13] 13[2 g3l 23 3i s) 12]13] [33]] [I3E]] [[#38 gHl EE[3 i [5[eElLEERERE FREER . 2 [TTT EEERER z 2 [2[eelsl[elses]l[o[ez]e[le]zq] g FETTERRRR & [5[a[al es]zl [ala] [e elses ] ]A] fig 5 i = TT EERE HS gHz IEIBEL EEEHEEEL ENe=EEeEEn E i 3: x HLILEELBELEIETETE]EETNaElEeRoER : 3 EEE EEE EEE F ii EL Teel 2 [2 lel][aff x EO ele p 3 EE 13 = = T : a1 5 5 2 i z 515 [2 3 3 5 gs 8 8 = 2] ss [A][]sE[:[lERERRERER i [E 8JafefL slelele[L [TE TT R] 2 THE LLL EET $l RRR 3 =LLL] RlElalalsfaialelefafs]s] $s1e [[[ 2 ELELl ]Fle e e[lTesTl Tle eR a[] I EEE $1 ER LL E eeeEaT a 0 EERE TTT]: i i: ERNRNANNBEREEEEEEEEERE : $3 (= J l=le=lele] TTT] ]4 i:(3[TLL eae le ee iF 2ii[(a2[(alTelT=leLeeFTTTeTeT]F OER bo HEHEHE] i gz] ]z 5 gz] [2 sg [2[EFlEEEEEE|RFRRERERE | EL 22 $19 [ELL z EE EREEA 5 [5 elala[a[aER[E]=|l|=[lEzEEE] |=5 4S [2[aE s[ER leaelaNaeR N]lE|Ee|N[lEaE alEelEez] -1H i $LN1] ELL TTTRfa Tefal 483 S4[Ta[EolLeaLe aIaIRRIATRIER]T|eIsellTaEETA ii a 5 i: [[2 e[ee[al=alleee|f[aJlaellsea 1] 1 ELelT e Jefe] 3 HEN EEEEEEEEEEEENNNEEEREE: 3FE[T 2 [eT lof eT Jeef] le e] Bf 3 LT Ela i 3 = FEE i 5 [5 8 5 8 Jz GE EH 82; 2 35 2 2:3 gs =3 i gE i = 8 5 gg 2E8 s 333 E 1N2E525EF& T23i8%: 2 a 5 (38 F ss: l# =: EE (8. 505k il Pf E F 838% 5 23 2 E2E3 825533 | |1R.|)Re|lc|lz5l|s7l|aBl|e3l|s3 2i:: 1; i - nimi iFi id 2 5 2 =|3 sla i ] i1f $HEEEREE2 2(|z3(|=2 =3 3 sf oi: 3 d 8{lhJz 2 5 [# - 33 [5 13a Iz EINEINEINE iz8 g$s5.: 2 5 3% 82 5 3 EH gs 2 7 o55s i& ||&3 Eeo 88on als 2 3 ' 2% " fri i $S 2 ,55528: &3 3 53 HY2 g : EE il: llei 23 ii $ 3 SSiflils% iBsL lHeElEa gollaa|las|lzs|l || 32 SEE i FEq 2EEole py i : 70d 4 "olsiedEB [|LlEelEalsEle .5 ) 1 si 5s 2 = i5: gHE E|O]IE [5IE] |[33 g3 i 30818 3 . 2% gs, . 8s ogy HH2] E2E2R3 z23z8]] a 332 H:i2 g$s:213 slg |= @ 2 gz f - [ig2s8F:22 g FF 3 a32|3|3|= HEE sll.) F< ECga 3 2 : E2%ig 5375(:9: 8 JE3f ol] % FLli : gi . 2 8i gLE= ii& i3 SE 5: 22 3 alslzl2].]. | 2 2la(3|z|2 z ER = 8 i Iz olsz Bg , E3d ie 35 [3 alslzl. 5T 2|2(s = 5$3Fa 83 32 3 z i::: :gz i ail HE EE 32 eBti3 gs8(lz8||z7[|s8l|s8 sed Ll, Eos FE i gEg 2- :i 54 [523 FREER i3 i ZEiils, 33 . 8S:ls[E837 (slaalsalse ss|lss|lsa|lz|zzl|ezl3e| | i2 FS ALE : <i5 gad= :E 2 85% i 1%3 $33238 |[[3.5l8. LL 2 HEHE 5f 2 E32 5 id :2 13 [32 I HEH 3 N= - a815az13 3 [3 If 2 8 [2 EF] $iz28 2 1 2 i 928 Ts : 8} Eo|Es gs $s 8 Eil3Es 533 % 2 File. z FEgilSi2as gE A 3 208282 2E7R5N3E;] = 22 [g8a & S3e23f|3 33 3 3 I it: 2 a2la3la8ls3lasil]3H45 23 1 i3 i 3i s5||88l|s8 2EI I ZF 23 & gl 2] 15] 3] |2 i z LE 15 LEE 3 2 8 Is] 8] |Z] [2 iz gs i: 321 (8 8 3 i2z ESF2 - 522 EX a3 81= > E=3 v S 3 To J[9z2:d : |FS2 aBlle3||83.|= -$5 8.8- ;35 EEd7i|3E5823dce NEii BE 2 E5iE(8s28 = &5 i2 E38 & SEF i 322d08835 |; A 2$35(53%:; |B 2 Z: g - 2 i: |sxd - 338 E 5 12 252 HEi= :3 gS i: a2 Hol 28 2 3 = ga 8 32 TEae= i 82% 52 : Ief| 253 g E 52E[2E2% S F=EE ] EEE]8 iIf] |352238 gE F3 5EE(|53g59a%%E STie223 & 3% 3 $ga878 |g 3322 A % ele 25 a22 lge i: ni5 z Z= 2-135i3 25 HiH: 5 = 733 SE 1 sla !2 siii]: 21 2 2: g3l 3]|3 ([23 3 Z| |Z] |3 33 3[z gl [2 iz3 gtd3 g -3 <= Ef 22 8 A 3 i 928|]. le] slelelslsls > 5g 2 22 S88 2 1 egss g |: 2 =~ 5 gS EN |52% S 3 EE38:2 Ez75o2g8 FLl3gd Ese [E78n 22 lgad QF g3 3 3% S[gEaSsq b3d " . 2 = i a $ B : = 5: Elegazf|, 5slz3)s5 5% 82 = i i 3 : HEHEHE RERERERE E55 [2] 13 [3 |F iz sls) 18] +[3] |Z] [3] [3 iz = 53 8 2 2 ii 838 25 2 11 PO] g[z3g 2f 53S 152% < . |32 % 53 (8:8 efi slalals LIEIRE 2SEE- i [se - 5 = - i HN a3 g2 Eilds 3la| 4: :2 $i(33 =3 s=2 <|g35258d3% 8: Z [32d iz RANE HEHEHE 12 EE5RET8] ii ig M3l2 :i zi g 2 3 81 8 3 328 oF gs 2 B23 92 E58 i [tes I9 EELE 58273 tile, isd & s|zs|x(z|s|s|2]a]s ; 3 =: a] | |e]2|23 oz z gs zB 7F 82i38s3= [ed E2 33is; 23e8 |ffg 5$8i 2 1#12 i :3 d3a1l8l3e2] ii, I3g 2%i:id - : Z gl 18 3 gl [3] |2 gil |[B3]] 33 I. _ i gt :ii2 EER sed I. ; : ~ 3 : 3 i ic 82 od =% sd 1 3g (#8388 SIA g $58 gCF! teE3E%S:: SE|i5d files -- [E78 22R0lE8e82E88 gi ELE ] - - =; elslelele]s Po i 2: i 42 [i353E ] : TTT HERE 1 i 31212080 E 3 3iid LL | i i i js G FH g| [8 g g i 12 ii =| [8] [8 i5z2 gSsET8 (als 2|=|als 3 z= = Z i4 EE5 = a2lll s:| gStzd 3T [|E&s8 I ry S3,0[3328% |. [FF 4zl3al85l5s f2 tiEE:El3REasE3 HRN 2 &: 3 gaEf ISk EE [33 - 2z < g3:i(3F33 - :i E3 E ga= 3 BE I =: [i38d3 |]ef a : g2 sz} " 1f 51 i52 dEs ::i g= 581t3 I 3 i 2z EH z gs gtd H5H gs i iz EE 3 23 3 33s ged sls E3 fo. = &= 85 [|9g2883 23 [F 8 3 #3 ga2iillg,5.Es8 |= 33 535 23 I iz u|3|a|z|a|s|a|=|2s1|3 5 z - s<27 1g(922%8 FREES Z gz 3% alz|sls 2 flea w]m 83 13 ii 2 ' - - 5533s - i 2 :: gl 18 a -g :i El| 23]] |32 2i 2% 5 gs 82% 5g 2EE:R 3 Hl 8i2:3 2: = Z32 ailNs 5 [es 2 f|8ss&3 ii 53 4228 Jii F5E3RE|EE2R iifF gr i $73 ; i ff I HEHEHEHEE i 3 2gFeirlgiEts 0 "UU s3e43 3=z|g28 3 15; 3 i led KER . sz - ole 1 ii = ii HTEEHBET=HRT=ERET= RET= RETe i 3 gl [2] 13] (5 18] [|55]| [183]] [|3]% ||% 3g3s o$2z8 [qs olals 3 2 = 2: 58 32 g 92d :2 [Edg 34328 a 2 { i sf|E 2 2 si Z iERiA liz HalEalBalEz R z $= I:20328= [ae E|ag F als.| : 2: E |sad sla 55 . 30s 3i 5 A3 f 22 s1 3 ii: #3: E8E 3 5 [82E8237a g Eg 28 pEI:lies Zest 8 il[iged SEE 8 2] lead ERNEER: lgzd HR if. HH i 8 [2 : iE i3 283 . 2252s ssl|saaRls|als| HEH HEE z T3 ala fi 3 4iats EiE, s=lg 5$8:3 2 iii i i eFz]I5|EoL|2Rl|aE|ell5a]| = 3 |3 53 gz _ i 8: i2$ 875s%= $8131 33%s 7 [328 |; PEL gg g (%28 2:55 SIE g5 az l(l38iE,ass8 [|3e FFF SELES I1lsd HRREER = 3 |gzd eFO|[3i38E:8 g Z 555 F 08s = 3 . .l 2 a 53 g Ha2E , nn H2i gz 22 i H%)l3[=z|i3=|2l|s3| E:s3 22i HH JE % FTE 3 _ 2 3 2 2: i HOHE [YE i si |g] |g] [#] [a s : iisz i$2s: 2 E54 2 = >2s E$3S228d s3f |. 22g AF 2 8:8 F2 PRE(EEERL B5 H2 > gE3iri(%d:8 I: 38i(gez $F (33% 3 i3[7 3s3 FF i (sed lig 2 st f 23 3 i 5 gl |g Z i (3 3 3 2 HHHEERE F2l2E/3R(aRlsE|sR]s i 3: : iI5z% i HH 8 g3 i: = gSse fz i i 3 z|8(e : HEE 5 il 3 g 3: gs 3 gz 22 2gzs2d3% I3. 2p2s 2 = 2 i}Z 5$2:7 |oflalslal|s8l|z8 aHlE sa $38 Igs |F%2 S [|3E:53% Fe e5l|EE2 2% _ 53 (928 FEREEREEF 2s i g5 [38S [777 X g E Es |= 23 5 :i @3 8% (8235 381080382 3i 12: 3g;i E[:is3i TTT] fr gz g 2$53%83 M41E3E3E3 - EZ. i= : 3g g5l 113 8[3] 2| 5 3 s| Is] |g] [2 2 33 $28 2 |s:s5 (7 5 3 2& 3i: v2 8= $s oe 8: Pl i $22338 5 [Fie ' 3E (8.3 sES 85[3%532%3 : 2 g 17%g 33 i822 1 EFiREEEEs g3|s| 22 ogz ya 8 iH 2 g 2 $: ER $R2E3EERME2 EfzgXz: "78> 32 "| ie1i52 = <0 32d i 5 [3% og z .. szi - i3 _ E5 N2 : z2| 3 i:< EE ni HE z| |zl 2|9s.l\s. 9528/5/50fH5gE5nEaEeEl H 2 ERE i 2H15E13H071E2)HE7)50 H8 H d 02(5] 33s71e51a33n] slslslslslsls 3131313|2I515 HERE REEFS dls ee 5 E g 1915/9/5/313 HIE ; Es ele .- viv EZIEZZRITIEESIzEIEEEEs EEIi E:cg iEeEg clEE e Ee ee g 25131513(313)315 aEg1e9es ziFFESETS Mh LER R E EEAEERAERETAE a|55|al8|8g2l)| z|a||e222g HEE RARE ol"8s21S88l1s(5l8s i EE HBE31ME8! e33a $i L 5 233555[g3 38 alr iN 882152/.31)3 gggl8)|= i= 3 = 7 EEN 31s BEiii$ aelsels HEERBE T#5\R)57E50E2| 8g 22 EE] EE slzlelzlslz 3 =f ST5Te INFEEEEE i HHEEEEEREE1a5l|i5l|s5f|g8 i ERI3EEoE,i1g8 i |il fo 48) 1 i FE3 o1 F33 (fig Hi1iE!R i5r2s8 H H(E L28 i5 f$Hi2i53 El] tEilie & FREEE aE 3 BP Lm i21: C E2S3I2EFR0R 0EE1H2E1R2HE[13HE 2)E711E35H15EE)2)15EE|5E) R|23E]5E5]EEE)E.R1E35E)EREE5]RR1EEI8EE7E8E]]E2E5 .E%iEg : Hs322E12|H125|(Ez5|(aHS1l5zE1|521l5e15|E15R2l15R5l1Olc5sl1R5le1E81E5laR1]5zlE5z1lEa5l1sE8le]] & & NxEEEEEEREEEE EEEESERTERE gid IIol4xhiiZ5Iz|EeIlEzIeIEzE 5s1e2i1z5e 52 5z 5el0z e8leegl2se] 4 |335/5/%/8/8 HEEEEEEHEE 2 Hz H 33835zg, 3BRE|12E|2 5 |358/%|3(3(38(3/8 Hgl RH|HglBzlEelHs I HEHEHE FFeEE3 [$32] IE F3 i$ : = lz] ]2 88= 3 3 [Es sls So |giz)gle|e|z(8|8 E55 "lew HERRERRERE 5|8|8|5(2|8|s al = < ale ~ Eds: 32a Hi i i i iE 3 sig z clslslslalslslzlalelolalals)s| ES233FkBETE3 HE 33 3 g|3|5(3[3|8|3 22 5e 37 =i 2 iit5i2s8 ale =a za s Ef 2 i be EEE aalefsfelelele] fii z i55 = 8 a 2 |EREEEE: i 3 a7 588 1: 8 vvsi?g |F J 3 [F3F 7 v EI2 2HE5/E9H/5E18E1E8)H2E(8E|8HE 583 HEH EEEEEE EEEE E EEEEER 55131518 33 z i: di5 na|2tlel3e|a5l33/25|e2ee|5|c|]l22as|]3l)o5a|s]5e] iE 318 g HEE S$, [5] $1515303050505 51815 2s 8 6 5i:5 |3a1gl5elg12z.1 |2|812]_|ze) E|5]8 HEHEHE HEEIE ES $Fi1l3s58le g=]l3)2|5|]8z(]2z 3 ]2|82|0|3z(.2(08|02 oEHTE E325 EiEtE: is: si! ERE gg" 1HE:] g3a s lB lalel | E2E3RlE8 ells [sls] slglz|gls J [else] Telels] g1g5l|g5l]sz15/8l8 uh2fr1e%d H8E3H8 3 gi8z 2? H= EHEHE B0a5 i i : [[Bslgaslesz fslee=efealsezl c31hi aiEs : gfi od Gegenaalae11s ii tig E2HEEREEE g HBi iFE a33 is 8 lP sgO sls|3|3522 Es :5 EHEEHE:HE 2/3s1|2a1l2zlsele]z] L2 l ~ E73 o l[ifsjssiigslTEIEEIH3EEiei3l1e3EL 53 SJ5T a [E[elelee le g/gJ 1 di ii i SHE HEH ZIZIEIEIEIE EE sll did 3 iu Wi : &ola[gEEajajz)2 ss ;zSi538i Fo EERE IFLR i :aleld el o-ls :sg R[0L ) 1 i I i 3 2 iy 58a3]le2|128](e5lla2l)E8lalc|88212g 5:7215 5 HiiEiHla 4 mn iig3 j | 3 233 ; Talleles ii iss i :i PREF [I= E[EelE[el E Efe e[aee Tr i S855) aiiid a E ss i 83 gz - am. i)a sls z z :% g/g g HE HEslRg) 523 & i18 EE gs { iHH = i12815135327(2)5 7 08 LE 2505 f J e gid H5E22T |7|7 05E 2 820g323003185s)ui 4 i I hae Life 53 333 7 FE figs 51232 i Sg[FEE ki - : i! 2 PEREERH S sls g i 2S}i I%8 57 EelE s T : i |3ig(g)sls i: ii i FE i 8 z fo gig |g 15R5.R)E. 1R2EEf EREl i LE: HS|aalRIeF IFi 3 3 i HE iA | :[E faaaefE sleElsfafeles] ASihid : | zZ 7% 2i2 i33; 2iE$H3E1(55SH|1I5]5SH2])(505I5151g533)l /75)38|g a 3slzl5sls5 815g1g513 5BE:PReR8g] gg i5: HEREC EEE :.15g1i5g].15g1l58/|2g/l3s)312|2(5|5 5/5122 (gla) PRET EERRE 4 F$A3RE EEE HE EERRECRE EERE PRN HE HHERHEER = |8i53]5/5/5/3)5|215|2/5/7/5/5/5|7 8158] 2:0; = 5 |3i83)2(55802)2(2 5 a)2 8 el2e5 EEE) 2 :g iiE iii 324 id. I=iE|3Eig(Esle gElzE(zR|3E] ole n3L EAA Ase 1 MEINE ENHEEEEERRRRRREEEEEER 3 HH HEIEIEIEIE 2l2|d|s 2 i2i5f 3 : g IE slzlale]s i:i8 i82 , = Jee T: ii .Eg i: is si. 215025 8533(3|3)7 it & | |3]35]7 22 2 : is [818 3: - : Jp .%_|58|8/8|5|8/8/5|8 337 pp : 3 pppeppep BE 2138/5/155)(2S(5(8(2 5) g g g ggl2gll8eIle5l|g3| 2Zcf g :3 * is { 598 7 g 58 gi: RH EHLEEHLL EERE EEE Sy 7d 5/8 59 HHHEHHE 33 I0HN(55a0E801E00E80 0R8. EE58 i 25 E(358 315597 8 3g 3 4 ig gdsi si1d}= i:2 JERRREHERREEHEE Fit i LS 3 : CI 8 HE ; ! le gs slslslz] 2151215). 83 25153 RHE CE FL al |= 31s |5ls| vv f3E fg 2355 25 HEH 1 : 2J3)s(z 5 3 I =z = Deleleleeee]ele]alalafefefefefe] Jie 335502s% ZEEEIEIEIEIEIER Fi i iizf Ta 2 5.02 5$55 RgiEzlEs a5 3S -- HPo. ES] gslesls cl|e2igllEs|lsE) |s3l| 3= 318 IEREREHEEEECHIE v v|v|7|7 VE 3 ofEE HHEHERECHERHEREE |z|alals|s(5|slslelslsla]slels cle]z] FEE] clzlg|g|g s sls|s|s 8 S CIEE 717|7(7|7]7(3|3[3)7 S45 I i|.83e|sl5els5|s|5s|e5||eaz|<]e]5els E]seHllEsEeEle]EE2E3 o [EEE I 35E 5 BARE eg ae i$0:3.|535a]a]4|z5l(z5|]z5lIz8|(55l128|].15|]55l(a3l]zl3s(l3z)l5s]]5e)].|5]|e if si3:1 i el 01 5 (382 55/888 gl5|518/8|8ls|8| 553 i [a S53ETTERg iirFe RE aes g slg 2331 S3Es g g38 po lFee lslelelelelsfalaleleleleeEeE] Hb z g if Zz g a3 HH A5HE|2zRlg|N| [gH]eElslHs] RTEel NTsE] IE 2 :5/81812|7|-|8|8|28|8|5|8|3|8|3|8 iE os "3 55 35137) 5] 5] [7 121:s2 FE 3 I: REA EEE Z| 9 EEA g g|g gl 55 i EzIRgP A5HE|sM|2|s|zElzE|Ez|aH |2|5R |a|E ele|E aEl2EE laEl2E ]s] i EEE EERE REs snes nazz i S2 EAR 3131331313315 31315513055 5133 isi E2H3130aI8z|sl8e|lSa|alx|[SIlllz1zl(aal3STolelS5 T=: 1 T2 d3i2f4 HtSElHelEe]EglaHERES 2 5 (358 3 a a a irpe SEER REE ds 3Eis 210 % 25 E8E 3E5S5E (58L |52 d 8a1lz5l8s/l3s(l8e|l2a|l8e sig < Es2 Eig:g [EE 221 HEfEgl2|z|z)a)5feSEHR EHEHE FisL LEG i F[elefleasallafealelleeleleeg] 1 of 1 E5/8|R)R HEHE EEE HEHE IH] 3 Ss | 23: i i. |glelslz(elzlz]z]z]z 25s slza| Ifif : 4 2 PF Eds as " gz 2i2 5 |= 19E|z(2 2 22310231H85E2B22 2E210E82E2ElRe2.0 f sls] c1188 591 E585 275|7| 00|7 E5313131505155 SHS gd ARiEmEann 3 12|515151515/5/51515151815)8an ii3 s $23/3/5l5| 5/55 le 8 5l8)2 Blelelae] | hlgifinggeeageelelaslael i 2 JE EEE EEE EE Eee ii 8 g151lgl8l3i5l5l5l5 55 5 5 a fi 2 232222]2|2| 2 sl5l5/513 HE 3le if S58 3[3|3|5 Ui 3 i: 5 ls 2/g/8|8|2g 22.0. PEa R ER ele SE %& |7| FFF gH|gE|gHlgElg] EIETEIEETI o 5fd, f i i Sfilgss: 2 gB(22))2zlR8(2E|8E(E5|) 5313g :FSayige ol3g 222 g| |alzlsls oS Eee [el] Bi] 3Ii180 dy 3 EINEIEIEE ig 3 5E53{83(331sR)|:5|5)2 g 2(8|8s]l%s|l3z|l3s i2i 2H5d i: AEEEEEEERE 2 a8g2 :5 EH3 RAEEEREEEEREEEE Fo sl i 3i8g Ziisi E:15(88|55(55(3)2(35(35102]82/ Ss |7|| [vv B5/5I050 gE vv $E OE3 333 i: ! 53/5/8|5(5(5(515/8/5(81815(8/5(5]5 518] ERE EEE Ea EEE a 2 2 H |i. E8RlzEelEe EeE leE) EelEeEe EalEeEe EaE2 ele]e] EEE 2 sF 208 FEEFRPEEFRRER ES i! i i $fAE188E/8/E82E(2E8E)3E| NE|3|NE|a|EeE|eE|e|EslEs]Es H 5 1k 8558B /8)38 5 5088I5151811818918185185133)3)s Fi; dF [ei 1: g5g[E|8E a ala 815/53 ET1f HiEE- D 12:2 iE #2 3 5 |e |gEgl2 gle Zl HEH Z2|I2lels2g g|8|8|8 is 23 22%:3588 igs FN SHEEEERRREEREEEEEEHE: 3 55) i J= z a EIN EIEI ERE ia s2 HEE iii: fi EZ 5gfif PL w 1 2 5 [e 335202| H2202E02 RE2ENEENERRHEERHEERERI IE 535 ash |7 3B7 71338 18 Z ; 4 AEE EEEHEEEEE EE HH RE i 5 BEEEHB 21818 8H18EH21E51H 515E15 2 7 $:2(3|8 g| g 85 die LEY gg2 3Ergsair3fl5 25322i/T55z5z)5 sSsEi|5glg]eS|llEszisESn(lsz 5zg2eHs|5lEs25lgEs| EfE2iLi:E:3 EEE EEE EE RE Sg|F8131313)3)3)3]3)3)F 1 iHP1 HrHiH3a5EE4s)3EhE)0ENEnEH1E3.REaEgH2Re2HRRE238LEgsEIRVEER5HR|HE5(E2 S =& 7] [7] || Fi; JL 2EERE15s al3|8(5 HHENRHEE 1] iHE5d1 s i: Ed iHiEl Z eis 2 5122127 2/3175 222 23 55 iif it 8 g3 3 HEE I Fk Aaa Jl i te [Er ] | gSNiEnIBBEBE RE AE E H2H: is :i ; 88 82 ga Lege EEE ee EfeE i: LHH H HEHEHE ERE 13 [9] |7 EF9R7[7E|5]EHF|7|7)E5] E 2 5i HEHE RHEHEHE EEHE H 85/81128|152|15E 3|158]51|55|88|5E|5E E|5Egl5E|5El5E|2B E|2E|5E|2E|H5E]E5 RIEid s EREEEE EEEEEEEREEEE dEEtRsE 2:%|5|5|5/5/5|5/515|5|515|3!55|5|5|5|5 2 FF i 3 5d5 EB8E8 RE(8 (R8E|8E(8E(8E|8E(8E(8E|8E| R|E 524 SE13 E$s15282271713S1331031)3 [EEAEP 5 ol ln Fg3EE[2S5 3S o8 S 3= |E2LEZSg5I(EE|g1](a8|3|(g s|=|53I2E HFH HEEREHREEERE E 1S] IdNEE 32 ols 83 Bf5r3di%g E2sERE2] 5 ]a]e|2 gl |g 232 x|eE|e ElA0a3l7E]Els] 2kg%EsEE 5 FL i EEE iit ii (B[e>e[slefs[alls esllsees[guslless) I21ikiie sl si ii. . Ped 8$380 ||FiBRlieEEfzEEllzeRe||zeRglezEe)|zelzjlejaeel]eee||slaJel miEJelfEe] i252 s al lee ela ses elae lealal 2 3393212521E122 225 3212115 281 2 |RIR|R(2(8(%|%|2(R]|2]|58]|2|2(3[8(%|5|18 3 : LH SE HEHEHE EEE EHD -T |,1]eelslslslsls]e[e]e elslsslelelele] 22 21315/15/3|5|3|5|5|5/5|5|5|5|5|5|8|5|5 52 52 32 4 Sz 3S vv v v|v| v|(v 7 HER : A i: 3 |laf5g|dsll3slsglzs EsElzlEg| AE[sElelsEls|E|N 2332 3 55 5|3|3|3[2|28|5|2)3|2 5 EY je [ieee EERE] H a HEE alalz|z|8lg|8(a g EE i i 5 g HH EEE HERECERE 2i3. gis JiTT) | lTgolgll:e sTlgjL gjalEais i., 3= $i1i8 3% J7|7 33[7[77]7 $f oa HEEERRRREEEREEEERE 3EE:R 5055 sc lg El Ele aes 3 |. 2 |s|s|s|a|s|z|e|s|z|e|5)5]5l5)5]5)5|8| 1417i7|5007 2 \2 220) EEE Ee , F355) 5 5050505 5 5 5 8 BRE fs 3 |.8 gl lglg HEEEREB E To: E3iFaaRlz|sI(=z|E2S)h2=|z2 0 5T2|I&5E|R2xR|E2el o-e ver) aTfig[Lio iz2z818F(8F5% 8 &2 E1aEit:aEzlI2lEEgI]slC]eRc Z 2 38 Hi3 EHE al3| H21E2E1 als| 5lel RIBIE81 3 F =F|2|E|5lBEEzL/EE5]5o|R5s]Eo2|] 52% 2 if itd it: iE HE 0E E1 i55:8% i2ir3%is 83 3% 2225a 23e3s%2 [BEE Li if 33 3 255(%(3|3|3|5|E|3|3|3 3|8[2 FE BE Ey 5 i jii5i2:3 : Zlzlzlels|z2]z alas] 523 25 gg sFtF : : 3o~ if Er Hag JE 8la | |=g) lsgl5sllEa]s |ae g| {2 3Z8o Ss 333 HEEEEEEHNEE EEE EE EE 0 FEET EE i is S: iE:a HEE2Ei|z2|2/|85|%||285|2(l58]588/20l|18/8l88|885|815|E81E|55E8|518l5E85l|gaE)E&3 9 EEEHEHEHE EERE EE EE i5 BREE EHR HHHEEEEE 358 EEE 3HRH|HBEi5HEE|gEgElR ElgiE sEgEaE EEEEiEEF aEEEEEgREate 3 5S FFFFFFFE v|9| TEI) 89 8 27% : i 5 ||33::%3|5|5|5|22|5|5/8!3/5&l|58||53(|551|220]33)|851|55(|2a038 5588 5505 HHH 3 g 33 H1R1I 2 7 2E g[Si5E3 2 5(32|88 5 al 33 |g HEEBREHEE gl |g] = 2221s 18/85 |2(5!g55/2|5g1|33(|52]3](2|5as] = |5ls||5s|| $551 3 efi% fiiis e $33 oiEt. EEL 2358 23%d3:: {=i ( ; & IE JoiEr4i2s iE:H - " Prd3 22 2 152 elalelello E8250 R Rg TIREGE RR ng gl2 " i | 7 77 7 3 ERE. EEEEEEERREEERE :EE 8:5882|8)|3 /5|35(g8215g28l(515205 51550815] EFfS : 55051315 05 515 38 8 5 Ele ats i i FRE REEEEE HEHEHE HEHEHE E T S|18:h3/E8/l5/E8g|% EE18E|%/8E/e5|5E/5/E5/E5|e51e8|8| HifE: $e 78 FIEEFFEFEFFFF IPF] se H 31502|22E )55|5I0gN 5[s(l5s05R |g8]5|g)]5I 05[sellsn] |n|eB[E seEllse]) E 4iE3E8E RR 2 a="(EE 2ELE 15 |g |5ls | lalg| |= c |a3i3z|s|e|slgellg2llgE|l5e|]82lE]Seg1g|El|3(eos ga z sialsiolz|2lsls]alzlzl zz 25] 2 {TEER ii A i 3 g|3|8|g|8|8 g|g|2 2 ihTir2i22s 2158 i83%% s3kEEs: 5: 2% gig JlHfEiEl 5 5% go: ii 3 - TTT TT = i FE] i: 8i35]3/(2gE2]lIzEE5 ee gl 15 i 15] : 5 i fs Hi 22 57|7|83 JF i3 5 z a if:i : |213)3|5l3]3/8|5l2]28/]52]5l25]25 i o E$E3G13[)S(35)1551515) 5 0505 8 Ells iil T |. sleglesieigzjsissslialzsellsa] $F 2 [S 817B81E23I)1E32I(5E5015E300E75)1530230025521187132 57012 3E5l8e 8213 IE) " H2IB382|R2)E5 ERsRlaElsR]alEElE<lEEle zlalalz|g:lal iii HE 3 2 i51i3, S EIREEF 3 41 i2!1d 3 a= |g 7 az 5 3 CREE :i 2%i3|z2lal23[e.|2S s 3S J slzlals| g2|a5l2l|gElg]| 2H5E3E 2H2 d<8 ah2 z1i%g $1} i [Feel 3 aaa] Gin if zg 5 w EH L1.18(8)505)E El | 18 |2gzE] | 2 8 i [77777 7 77557 { 52(3|\35|(28\(85/(35\l55|l58/)58]32/35]158|31(5352]551l521)85]58]128] id at HEEHEEE HEHHEEE HEE sEo |.1|g|e|e|a|z|z|s|e|z|e|z|z|z5)czl]ez] if 1 HIRE EE 258 EE EEE REEEC EEE EEE E 2 RIMBE3I50R53AIE5E1E 5 g 3 El0 I3i5ale ISEEEEEERE1E5E 8E 18E $1 ERRATA le fig|2s3]8|5/5|5/5]31g5||5/5|5!8 3232 HglgElsElslBs| Z3 E 5|5H/8|E8|HEsi:I 1 5 8 2I1 E|i3iEzlEa|E2 ocE 217 | IF g HEHEHE ERE B < |Z = % HalHe|ES|Ea|EslEs| J EAE EE FEEEE 33 E22 id5:88 RE 1 EE5 8 i (be[EEE z 2 2|g|2|8|8|8 Pi ee ele EEE ptve:e EE A ii 1.20),RE2CeEeR.ERARSECHOEE. i LH 3a5 | [F[F]F]7] |F slslslslelslslele Ty sl82150E851215) BlaRaBEE fi o Zelzgcizciziele Id HEE HEEEHEE2 sHieEeEee i HE SJE EEL HE i HE L aY li 358 i i Ee: SE Llelsle 3|2(s|3|2(3]3/3]z UNIitTE ii o RNREeET 2 1218/3]5]3x[e[2 212) Hh ; 82 83 {Eee lee th 5 aigz Tron;Bo8, i Jil gl le HRRENERE REE EE 23 2 37 7 EREREIREEIR 2 : HEEEEEREEBEEEE EERE RE EERE HE FREBEREEHEEEEEEEHEEE LS2RH 4 S |3[iFEz7H /133||55|H |35(/55L |75/35H |[57])53H /)53||55EE )!35E)]35H /E515EE /5!E5E /5E|8 Fa A IERREEENEE 59 CR 5: |3E :2]2/E 5|3/5/25E/E8 ME]HHH 3 S3SE: |=3 7 77 797 2F1l (38g alzla|selela|2]s]s]s]e g 8858 HEHEENHB HE EiE] EEE 4 EE5E!3 FE 1 i 32d i$3l1. 3s3i:g # ii153: sig : 5 P! a | |g |2Ezle|2lgls]al3s ESERIEJ 7 3353 HE gs | lglg a|3|3)5|5/5515(5] SE317)5)EES] |3(2 RIESE i138 31d: ii 555 53 3 HE 7$B [e- leeelE=]lel[lelez]eEellezl]eselleellleeleelleze]fge|) ga2n% $kI2e 2 3 i2:8 PA I A HH SE IEE) 1818] EE |g E1L1S8 18 i E17 7 393 gg gHaEtEHHHEEHEEEHEERHEEE EE EE E HEEHEEE EI5o (3|5.g8.R|R2E02E|RzE|2)Egl)zC|I5E)E55)RzE5|5E50E]555E|L85E) &pg E2EEllgof og f ol ds ldESEEESs EEzEs BEeEeeEE 2ER400:si. | 21l5al1al885|= 5532 EIEIEIRIE] .g SEES EE 218|3|% 8 sls IV ' EE 4%5 aEtisg siti5 3i5i3 i HER ES0R|BHEEEE|33/e5lz0lz0|27ale|s|sxs|a|eslsalaen|s]ns)2x]s|ag) 3a 3S 38 -:N e2 1 F3E if2r3i5:: 212%4:% 237 3 ul 852 EH 3 E3 1 HEEERHEE HHEEEH 23 = glse |z| if&r25if {be EeeEEfefefeleeleleleeelg] gfeila : EH ; a3 Zgii:zsz :5 sAl C|Rg ELGOEgOEzRRRRDRgE l R|gEJ|dAR:fEr82 a ig 221312228 558 i % = IF 777 :i -: HARE ERRHHEHEEHEHRRERR HEBEEHEERREBBHEEEHEEER3IR31E21R3| i(e(z 5855 els g2|5 flags i 8 : ip 3, PEE en 1 21 533 22f1I2e1l2E1E5z|5e58i5/z)5i8z/i25e/e2l/e2/2e)e9|)9J/il92s1ls2g|/se9l/ee2| Ei HE5if PRE MEIEIEIEIEIEIEIE : ji! EF: $3 2 [8 gl gl |8 2Is2ls | 2|2|z|l2s||2z RHEE EiLiE|3s85E/5]|5f(<|i5 al SEER 7 3 5 HE 3|25 i:is. |iits; 2Be8l |3%g|ss]al=zl 723585352 allele 282 32 afe| 1i [51d FA2: 5: -- gl |g 5H1E5.R1E2 AE H2TE=% Ess) HF Foletnslseff 18|5 HE g E7 77 fe Biss i F2(E3(E3(Fz(Ra)EalEel]e| 145% ii (B[Le+lisl[sifaEulels|lafHeefleeleEles][saefsfafal] 4 dIir bisg :i EZ i FR 53 =o g\gls HH i3lz)2/8(5) HHH 2: HEHEERERR i Huai. E=iEEEEE 3]15E 150g:R 5)E 5 aee5:E18s 13 glsl3|5)8/5l8 SFZ elH3eitzl|:sHlsEE i $i 2 gl |g HR 1s ~E5 R7 LE FHilEs s:|t|z|3/3-]s i 3 gz !rg HE I : 3 3 [i HEHE ies S888 31/153/53/35o /3/8/r 38||3 3282 ih 1H81H781739/571z3l13235 1g.18 ig : ].]813 253 i falzl5)3 i &,d5 i gi t iH gz 83511 igili; HEis I1H3 C Hl R : i iiii 3i= i . igz s5a[ls d/]7[o Z HERERERERER EHEEE] n5o LB55E2)5E)2)R8 8 i H RslCzRlgE| 63 3if. 35 |2(8(2]5|8 2(z(gls sls 5FI W :$225(1|188/11E 0(55(l88/81518g| i2 gs E3E 3 8 i 7ELE[ls slslzelelslle]e ai:f , |o 3iglslesZIZI1E1I523/I521I51515)2E gis ih Fa = 2(7|7|7 ie 15 (8R1E82)E5)4E8/%)8\5]5 R518E(818/E5]e| BSi s F1818 403): Ia| S[3 H) h$i2l i3 Id z ; Bisids: 333sEICeIleECfEaEle) 3f1ed | [FE Hl 23]. 12 z 1 HEHE 22058 3 5 |%| |2 3 : = [3[g]3l3 EEE i 3 2lz(gls f$1i4e2 fil if: g@ 3E8g $0iiz: 2 Fi ag g3 i RT ART EET EEH ;Po8a g gl alg 2 HE :3(7|5|8(5/5|1|z|a|2|5|52|a]2(5]2| 55 3 9 FEUER EERE fe I v v +3.15I5(21518)8|5|5|2|2/3/5|5|5] |g Bele 5 #si3z3188g < HfElHoElHcE|o|EclEalaElEala E8HE v :i a|8| &5 < EER 25 _||s|/z8)l1z8l]s8 |33:i8z5/l2|8Z|2S gs|l2s 25152 gs(lge|l1e8l|8e 5252/2525 HoElI ZZ E5R:] S82 55 22 I:31 |3a5h3l/3aa5i(El8i0na5e(see1.2 i 6 EEEE B015].= E85E1s5eSlEeE5lelnlss58] 3[5)3 | ER EE 212 35d Ei4esi1dds fi3 g2s 0,585Zs 08 | [SIgEg)E]. 53[g|8 Sila 1g2l | [2a] | |Js|lSs||Ss|lss)| i% 8% 23713%% zls|8 sels 2338 Eggs FI EE EEAREEEA ERRERE REE: 3 283|a " 55= 3 - lal 2 HEIN A ARNE Ra eEaen 52 3 f1Fg1h&t3 i - zlgls glz[g]s 1144 gE? EgEz [3/52SRE215E] RE18) 2HaR)ERE; ii 5 . I PiE d Engi TF Eg 300 PERE EERE iy iE:y 5s l7s HEHEHslEzlzlHzlH= Bele 332/2(4|2(215)51E]215)2 g13lE5520 i i 33 asa: RE] 3 8 Je ii 3LiH SE RE E: $E4i07m8 e[e7)3e]27)291.1518 3 i 3is 2|2[5la]5 33131313133 3s/l3s(2(:3)1]s Hih9li i Hi; . EFelEs "3555 TL eh HI E left 38 fa Ii aEz: [E18 S a sles 1 sHlsERE5 REBz|EsB|sEsR ji iii ; ERIS Th 3 2a oe fale fg BE 3 E8:L3 3[3]3[3|8 iil hi i iI i: [Be [Eel2Ss|EleeleElEasE-f} elaaElEe h4- hy; 8223 2i2 p glmglgazZSeHEaEEnEaEEgEEl R2EHME HEE2eaEEEnHElgEEEHeEEEREi f:PodriiEeFgas H22i 2S25 233E)H1H37E1f3EEI1eEH5IdEEIEiHIEEEIEH:aEaEnnaA an: i i: : | is 3 TEE Hh = 1HL=y5Eg0E2Ig;0I5zn5EgI8sE9gnce8ieEl1e5lgg1elel55le50lz8ee/ll3ellE5elE 5el50i2d2 ele i git 4 2 3325(2212tE gs EEE fHi i 51 2 Ss H g|v lg|18)1l5a)2l|2 2gl H |glHsR 52g1512 f: ai] a UREENEEEENE EE SzEPH 53i5deHEFEF E33s5)]3 2F0el|s5ials]e sa(laallss|ss [5 ES &4 atie g gig | |5l | ol 15] g:le gslea 5 58 3 33]: [F)7 i1ih: 8:Sl25l2zl2x2]2|2|| E f Ii |Jge)s i foalsa]5 12H11i53l]8 g/g 51 872 i il [FF Ii 2i3[n 2 : Bi i 2 gla =z|s ii { ||Z E h| yEHE HEREAEEAEAEHRE tiiihil: 3 22 AEE HEEEREREEE EF: 3 gi% g18|3 HEEEEREEE : ie . HF1ASEHEEEHEEEEHHEHEEEEEH| RERgFHEBFEeEReEREEEsRHReER] Is fsys $E5 3T30.s5i l[z5iag)] lle2llagl)z2a)lea Pos l7212|2 aEt8 |.%. z 5 [SE%E|s|s|2 ZG= g z: ER gT| z|g gf2E 55 85280g 1 oy9z3d Si1f1 -= &fil33e" ol5alalz E22 EE : iEF | (Fl 1+ s8h8y 83 s as|lss(l#s iia5 i Fr E:2A1E3(R3/E2(R3|E2(E2(E5(R3/E5E/5E15E5E2RE31515] F1E3 $050 FEECREEERENE 9 2 2 zi cyt LEER EE30555S sae R HEREHEEEHEEEEEEEEEEEEEREEEE EREEE gsl(z8l|s8 ai g Ld - Elid loz|else iz zl ele el ele] eeele] 881 5 E EE[8E%5(5 25311531355)55)3g)l95)|85|5/25!85|5 35i4 2: lalallala aa iz H55E0aE38i38Rn8EReERg E1%F R2R81818g) 8g|g8l1g8|0s| a$siiz d ils 29 53a]als 2 s3 Ll i1f3 E2855 g HEHEHEHEHEEEE 3~ E2g8 Seat EHbEERNE ENEERERERE EERRRR HEL ST EZ 1 gills BEER z = eHlEzlBalExlRs 1FE1a8y: PE [eee eee] i 2 8 5g as FgHs 5 sl |s 3 sls HH 2821215] 5|Sle]2|2 IZ . fon sls sels 2i41388 1 3 . 3 g15|5|8 252202258 le Ea =EEHEE51505)S HEH I5E 1518H151E88151)ERE =. 3S 33[7]7 18151505151515535 1 HI I|N, E8[ResElEe[EslEslEeR[sE]eEleE] eEEeEelEl eeEese EiEiE: fgEoHr1|i3.stel]eElsesla |HHEFREEEiiEEEEEEEEEEElERFlEREEEEi51Sy2i1. SE 5 33 5138 HEHEHE ii FFEiEPE5AE88 sH H lsl=ls 3 f1s3 a = |Z 3 Ifis 235 ,8.= 88l518| _|glg| TE 8/3|8ER 3 FEE HH |s[g|ssllss] [gsjl5gl].lel.[]a] | 232231%54:% es Sg|g|l3|ge|g e 1IH1k HHE 53 J igi 3 332)3)5)2 2 HEH I ii z EHECEERERREAREEEEER : RHEBEEREEEEE EERE =|E[E|E[E]2 3I:E3S; af is FiHf i5 2% iz/2(a[2]e g|s|slz| |2| |z5ls] [IE] & 58 i2 E3 E|E z|5E|E5R(EElEEE]2Tl2E] 2]82EE28 38 Ed $EiE3S/Ec/S3E(zE/E2E]2sR)s8E(s2REtaEtReslEzR|SRlEsE BgFlEEslEEs| iaE L% -FEALE REEEE ERE REE EEE ERaI8E HH EEEEEE FREER EERE So FEE ERA TE a Er HE8(3A5E2R/E5ER25a ulagl2]s Bg EgN lzlz|E$534E S$sRE8(85|38 8 a 8g2 s1g$ls7|=75g8E 3713 }= Bs 3 g = 1838 i|EiEe HHH 8g 8 ie, |S ' 1 |s|g|e|e|e|s|sslz]s]5] [als i234 i354 eR I & 2hiz3|)g3|(S8|g|g|s|sls|S|els|s(Sag E Egiz2:4 | [EEE : 3 iii 1 gi5z5 8F3 g: gFaits { AEE EEE EERE 2gF 2 2 sl=lalzsle " 5 =lalelelsl=la E 2% g #3 88 5 FE EEE EEE FEHEE ERE EEE EH aE i: s 3iHi zEzElHsE|ESE|E2Hgl| EzlE2e|E|2gll2 eeH|e| ElHs2|EsllHsEEe| lgsl ER o: is ; THF |.2R|HsE|sE|sHlsH|sE|sElaH|Es|HeE|lee|Hla2lleEs]]5E] 3 3R3. E5E|E s|glEslsE |zlelzElzElzleElzElzEleE | E|gleElelEs| i 853ig 38131815 BEE Eg EE = 9558 321 H&E3 ii2f4s3 BRE ga ns ER 8s giz & 157 EE nn 3 55 HEEHEEEEHE 7 5:23 iz gd HEEB ES] EEEER EE 33 3 HEgE 1 2E3328]|w7||cRl|al3e|]%e]al8s||5|=5||=3|e9]|=3||3=|l9a|3l|e5]|x9|]5e)l7 B25833 gle EE[Elz[ee]gE]selgEleze|eslez|eEz|eEll2E]es] G2v% 2% 3 g 4Ef 58 5 333 ai 2 az 2 TT] 5 25.0 50a J 2 zg is 38 g 21818121813|%(8|15/818| i < 8 DORE EER fi 2 3! $820:5(5|21515 HEEE5EE21E21E5 EEIEE E5 E5ESIEE EELEE E| REEEk 8 v[|7|7| v9 V|7|F JV (977 24 REE3 3050 RRR RE Rls) dg SIoeEsi5ESg818H58E5 H EEEE E EEEH Eg EeE Ee EEE 1 53 32 H8E5 |2E|E:RE33/E8E8z(E(81sE8/|R8s/E8S(/l5SRs|E|5E|SSlES||RES|& EE8]R&5aE5]E ERE S| 5SF1%2 . 22 2E0Els 2855] 88 S= =55[]= allslala]E=le i3:2 513|8|5/3) 833 g HH gi1z393 5 H3H)E5H81E5H0E8E2HHE gaRiS f3E2 1 88 3 2|F[F|3]2 s[2|5[% Fi i [Ele eee HH HEHEEEEEHEHHEEHHE EL g2 = HH i: BHR5AA2E1H7E1RN21EE2EE12EE12EE12EE12EE1E2E)EE9)RM%/EE5EE18EE(/EE3(EE2 ii: LEI EIEIEIEIEIEIEIE 355138 255-|1z5((z2l13a1l2z1|2s13l]a1a2]1s21]3s1l2s1|5|e1]3e1l5|=5e|8e|l8e|]82I]8e| EERE EEE FREER 8:3(3|5/8|5|8/2|8|8|8 so|e5 Tee 2Tz|xia2[|e2lsIl2sIlEelIsE]L21Ts2lsl2e1la2l1e1lelElIeElIzGlIeGl|e5la8] 2 3:83/5/%|3/8/2|3|5|5|8|55|5|515|5]85| BE, $i 383 i: sis Eg 3id id 3: 5 iii 3 HEE EEENNR Tr BiEEGIEEE Eg c EE EE eel Ei ~ dk ela|2slals|z|zle|s/als| eal as| EE HE = glee aaa 32 i2 : 3 5[8353lgl5g])215 5[gae SE3E5SE15)3 ls es 5 s21|2s1|2a1l2s) sEiEsLi) IR EEseRt EE)ly [ER] TE E E git 32 3 iri. i131 E20 E53 fgaet _ 832 lalz|3 3laz|z slalzlsls| d$FEESE= i FET TT RENN FE :7 [ E Be EEal eleee afalels eElgR] AsiSpd 2 it 2gg : slalse| [so] [e[efele] Telefe] [Te] gf:8 HH [F775] [7] |7|55]3] |3[5]3 iE 2 EEE EEREEEE EE EEE EEE 2138813el05da0ta5s ae :I|.2|si|i5|3s|13|nz)|3elzl|zele| H2HsEElE5]Es sH]sEEeHlseE] EiLE S757 EIEIEIEIE 3: EIEIEIEIEIEIEIEIEIE io F< ER1 3iPE 4lsls/5sI|2zlzE|Ez|E2|2R]I5E|2E]5E] 5E]25]125] 2& gi1:1 HEE EEE HEE RRR 51858 a 151515|5|8155(8 FE 3 [|52]130s||5g||s5llg5] 5 lg[5g1l5e15 5 0 5sl5s a |5= 2i:i7s Ept il g SEHEEBBEN EE g18|3 igsg p(B Eelaelees] HH i z 2 |3|5/35/3/3(5(5]8]8|5a]5]5]s Iris gl 182 3 55 2 oF 0i0z L5i23L2 3 EERHEE EllHeEEEREElHe EE ERid 33/93 3555 & 5 H23E3E03EEEEE EEEEEEE BEEBEE EEsEEE 6, E?P [AHTEoHEREC ERE ERE RE ERE 5f FEE[FEINE305E305EE3H38EEBEaEeEEEdEeRa aifns E7E|E3E0E515]5(2(8185508500 s5 e58e15 a5050e808 2EE2| 5 EE 58[35|3 3 @q 35 3 2 2&leiqHlSP3lER30EIH2)NIESH2EE0EL EHo ERE BlEEEBE IEELEEEBD bd Sie g3 i2 g4 fz EE ES B5r 2 5%3 5 8% 52% i:o Ube EaEl8]E3zlaelzeleeee3el3e3ea]ee] Giiibdd : N H4 i2 E . g0 f5 alii A988 505 5 1t2(e8|e5(8/5E/28E|5]E3/8/E8/E2|E8R|3|5H|8E|5R|3E|3/5| 3 iS 5ER< E $33) |alalzlzlzlgel1zl8a1818l(8/e8|ll8e/e8a/5e|5/e8|2l8lal8at]a] i I33 g HERE EEE EE EEE EE MEE ETE [||323zsi5i%5ls55)l/53[5/slEl3z5ll/s83]e HZsIZ|IZEzIElHelEeI lEeHlEEeEE lEEe2lIEeEEl|Ee2ElIEeERlEeL|| 25E83 EifEs 3 $5E17i1a(050.|5553.:0%1525|10zl5ll5(g5lE.s3(3/01285180 (8/s51l1gz5|l(8e8/l12z88/l112e25|l08e8|l 0 5 len | |T5e|l5e|l2e|l2e]l8e|] 51585 5] Ze 3 26B&331E&% . Ei TGf5 |.5325)5(3]32 2 : R$E07)83 2ER HREg ARRRE=HE = HEHE slsls| 37 017 I N=RE HE=HEEzHEEE EEEERENECEE i22C 53 ieriise EE EEE = 2$31522 1 ip 4 HEEINEH [5] 2[a ie | [Elle Eeeaafe] did 2 i HEIEIRER 2 s|3|8|8ls g Ez : z g 3? i 2 iif i2i:58 Hi J? 2 =3 $F igf 3LLigEfEe SFie dh Hse 2$$13i5 d|R5Ei)qss i S erp $ g: atas SH S i i, : 13 iis8 i ci i , $i i iiE i i: gif HisHiisic & 4 2 FE 3 33 it3 38 2f ot $3 Ez 2 2% 5 i3 g5 f3 z< i 5 z 3 H 3% g3s5 5 i ls) 2 8127132 |[22 si [8 2 [34]e| 5 cE = 32 "12 iE & Ele, | 518[258-;3 g #3 SFE SER z 2 g = ziz i3 & : - : 38 zs { 3: 43 g81c 53 8E3E 2x: FEN 5} i% 3 24 EH a2 Northwest Bioanalytical ReSptourdtyNNoo.. NNWWBBRSO00L--102656 Figure 1. Representative Calibration Curve for PFOS Ee eEeSNet i d Figure 2. Representative Calibration Curve for PFHS Aierp rtAoqaAeT C We So oamtTTn HS Page 105 000567 [-- RSetpoyNNoo NNWWBBRSDOLL0-2686 Figure 3. Representative Calibration Curve for PFOA Lm hans QuaLomi=19300 Figure 4. Representative Calibration Curve for PFOSAA TSm EE age 05 000568 Northwest Bioanalytical RSeptourdtyNNoo.. NNWWBBRSOOL0.-016268. Figure 5. Representative Calibration Curve for PFOSA a aGNmWapag sSRe S:A Cwm wm mm mw Figure 6. Representative Calibration Curve for M556 G ST a Neg ge ww 7] Page 107 000569 snetpe--o--n Figure 7. Representative Calibration Curve for M570 | =7 000570 Sorter Stompin RSuedNNopo.NNWoWBBRSr0O01-t102656 Figure 8. Human Plasma Blank for PFOS tSE=rs eree ,on) Em = iSTEeEREE dHEee%4 EE 3 forse | gE ee Eee ibis aay ee fmm ef GOES BE LTR ia EE Ta, Sh Eh Sh Se E ------E ---- E -- E= ESSEERRE EEEET- EE 2 [EP famland 0m TTR : ae E ae w--i | BSio; Ef gE r si a he Gc] ol Eg Eepnastoeg nal a|l ee=: Te Ph OV oT: Ye Wr Page109 000571 [-- Figure 9. Human Plasma Blank for PFHS RSeptourdty NNoo.. NNWWBBRSOO0L--102686 ppE ferodo--dE a--t W"E) S r----- a wQp=um enenr=e E [yka3] 0s mE 2 E I e EI I wenaa = | laa f E ea e t e Spans Bal E = mmi is wl] eas ea Gl pal ra CTE SR er ee -_------ = ,S a,.rE esE % a arn waeme= PsE Em fE E e e u e bo is oEE c=---=n an |m hi iAaE s] hE fe = To Br Bh Th Se ST Page 110 000572 Northwest Bioanalytical ReSptourdtyNNoo., NNWWBBRSO0L0-.102686 Figure 10. Human Plasma Blank for PFOA m aemee ntee amoEeemEE a eE emymE e os i Re Ju Col [et EE i f e----E r --w ---- E -- mma lisb4iniLEa gSahoeldie oaSesead lm eine Gee Elosfl0 To Flie V0 i "al ERR i ERE E amaE s . E saenE eE sdT2 -- wmnmE EEES Bg) Loin fa ameJaw Gl ma AT wofll La EhRael ahe BB E EE e L n ele i[10cCpllh eie lne e a E-- mm Gea Gl t #a EEE 000573 Pagelit Norwest Bostic BSocuduNog.iWvtWsBiSsIe2n8d Figure 11. Human Plasma Blank for PFOSAA e E n oornr n E = --S=--EEn EE(SR EERS,"8 [Er---------------- [Copia 2m] a GEES Bl ef Ry 23 agi | GT CARO AR Ter [o= o--] -- So= TEEEAEEAxE=EEER: Emm ee , fE B mmt e o Jl bie EE E [iommtL oe----om | AEE OA TET S 1 !wt WoT Wh Gh Te WT 000574 Northwest Bioanalytical ReSptourdtyNNoo.. NNWWBSB0R0-O0162I86 Figure 12. Human Plasma Blank for PFOSA -- J------ o ee EBen ade ---- wsimrmPebEt3 EE r e Jd fi olihoai Been TE f = e JaEd uc=gongs oa CTRL ST er T= mE -- wC S si=tem aebY f 22 ERTIES ET ---- fmm awi Lm TE Ea wife sie e|ne Li [Emor ers-- om--a 3 o tBh a Ee TARE euec=--- gerng : HBr Th Thr . page 113 000575 onhwes int -- -- Figure 13. Human Plasma Blank for M556 B E= r BE eE or n) = = tI2Se0 nE2EEEHEL,S3 Em e JS E Ee E l rm Eames temof [Ee TEih OEE SA] > i i if ml el LIAL i SEE Na Ah NIG er a. pE reEE -- E= TeZ=EEdh5EE"Y3 HT [EEE Jw i wi ean ras re |< Si AS a boi ie Epae te --wp ll| Shore SLfot Eat Gem flee om} 1m mol ame A BE = T Yo Th Uo Te her I 000576 Northwest Bioanal RepSotraty NNoo,.NNWWBSBOR0-O1026L55 Figure 14. Human Plasma Blank for MS70 B SeT. on 2 EEx mgeyn Wm SEEb mE Jd J ---------- -- [Emelra STATE B Eee all E [ocm =-fema ERE he ee ole bea e i goSgHl Tei CFeal Ri R E ==E =f FR soarr ems ME CE -- E r -- Ea lmmaonnae EE 4] ShEyamtg im EE i J ot wh WO ST er [a 000577 Norte Bian ReSpoyNNooNNWWBBRS0O0L.0132 Figure 15. Human Plasma Blank with [nternal Standard (QC0) for PFOS E me e men f = SSS riEEEeEiS.8 EEG ---- mn -- [--ee E EsE mex lo |Cr---- HEESie 15 nea me as ad CTR Ay | -- = mE) E o 2 SSSmEEEn fEIEETE2 a Er -- [J Rm ota wy ge imBS LERTE fmm fli ees Emm Jn [p ome u} f ACn CS E LRE GRS Ea E BeE lial eya Beal he as = oc=--y= ig: SEE er roe 116 000578 Norte Biosai PSitsyduNod. vNiWBnSiOoO-w1s2d0 Fisk - t Rm[ , S EnTeOm en a So-- mEExme--E5Eed--% e Figure 16. Human Plasma Blank with Internal Standard (QCO) for PFHS E B Eme m l laEtdiiy Ba E=EEl El vals L of ae hoym EEE CUSTER ey ETeV on Em t -- e SSEEEREEE r J a SiR EE |e EH mm PSE EECSeons ne E Etd ] i : Ea ic : STE er Faget 000579 Norwest Bioanalytical RepSoturdtyNNoo.. NNWWBBRSOODI-102696 Figure 17. Human Plasma Blank with Internal Standard (QC) for PFOA --etm ne a ST E eE EeF fresstiaied s= m=bn rT = Lo cite ad E-- FrE---- Ia=l a sa oren s n iLniy a. i C CEaEm SEmAJeofw |e i cam ee wep dd|e REAR x : BOE iA (ill a Sr hn BRANT Yel EEEai rene E ni RE = m mE e = ar a wsnNmE see ye Emil wallhee Ee Een E-- Eee -- a gy B rT6i age ]ve Eae eis : fE =ET gb 4 EEE BE EEA Page 113 000530 Norihwes: Biosys ReSptoryt NNoo.. NNWWBBRSO01-102636 Figure 18. Human PlasmaBlank with Internal Standard (QC0) for PFOSAA mfseann mn o--smnmE = ee = E=wEe nt free rial ferme oT legs James=e Jo Anata FBLC aie [Sta [oe m GREEa BO w i h n CUR Th GR er ; C SemE meE s ) wr = my mnme = ba EEE n3 == Erm pEepe c sll oe Eat ope a peewr e inne s bd ata |rr--r-- hy et fe TH OES SA] = a Ye Ph WU Gn Te WE Pope 119 000581 Northwest Bicsrlyicat ReSpaodrtyNNoo.. NNWWBBRSO0L0--016265 Figure 19. Human Plasma Blank with Internal Standard (QCO) for PFOSA fS Jo m P = mm n 3T3 mn EmymmEb erm -------- TH aa E E Ema u R s los e o]s en Emme = CEE SE of | bie tJe H ally IIN NBEA SOT ATER ---- o = J ne e ER -- amy=b = E errsRoE a RomeIES]i] mt an Ee feE m man hai) =--S-- l ho enea De : TE Teh Ser Page 120 000582 -- nFAr vases Figure 20. Human Plasma Blank with Internal Standard (QC) for M556 m_ m ZESiRneo TnmEE= ITnE=XEEe5EEERYY"4 er = nn (ESE El [li Pi EESEa i ed Ee -- whe Nn WS Eo2 E=EnEEHTBEETY Peienrl o e om e mE B= EY f bre edPl Emm ORFS SE =| fe Le One Te i Eh VG Pe her a 000583 [---- ReSptourdtyNNoo.. NNWWBBSR0O0L-102686 Figure 21. Human Plasma Blank with Internal Standard (QC0) for M570 fa ori o = anfs5 mm tes --_-- w= rmmk y E en e PE -- F-- roo) 3, ad foEEE he = el ew tEE---- e Cl Bll =) Speo ala LHRIe mh SE Th Ba ey TE mE-- BeR ee T m--_e mase rn Emneeer |eTallel SEOL T a TE E re l r-- E = a e= l OEE SE = BlEeesainthd 5 3 Yo Ph G0 Pe nr Page 12 000584 [---- Figure 22. Low Standard for PFOS RSepoortyNo. NNWWBBRSO0I.102696 m --ega e nd E QJ uSEp enieEv [eRrTe aEdi]Iar =e mmm mn ee = o-- E E e d r-- n k ne Gia b Ean E pocoe CEfaEtes 2 Hed wood) al SSR A Re Tem1 -- o B SeEn e eEI SEE = a nell GeTd a E ae Emel on Sheil EE GREE BA Bron 3 x ERE Page 13 000535 Norwest Bioanal Figure 23. Low Standard for PFHS FE mCwnierwea ,e . mm S oeb m s EE S S ws=Pen RStuedyNNoop..NNoWWBBSRrOO0L-0t16265 be fatto] Sree a Se] AEE Gliiesal Gl =Sbr cp ee 7 lpr Ft Tart So Bh WE Te E -- =E a 7= ousSou= anhmamna ebee d enn i wl aS Ee E Em SE r Ce A er -- Teed| Dip fra el aE -- Ho : CEES SO] = py ENE RR Page 126 000586 Figure 24. Low Standard for PFOA ==U E= = OzZEE=eEF -- Ee E m aby Eada Sn bees pre--ar el ns [rmemamyyen30i ] TL st ETE [mwsonwine oeHf AE J SAE GA wv fhe Tha FR Ea = -- EJ z STZSEEEE= EEEEERKE" = [e Emi mletin mimh rn 4 A RE BES SE == fom fC GREE BO = :Iban Seba TET Sy 000587 Norwest Bioaalytat Figure 25. Low Standard for PEOSAA RSeptouryt NNoo.. NNWWBBSROD0!-102656 penyp-- s orem o = p= mran =i]s+ ra = E-- oer oo wo Eo Er -- m-- -- SRE es tloy y CRE Ol ef a Ln mE, zl ET Em... ET -- ee wS sinn emmb me mm mer fmf Sah) en folds dt am bin JERE fmf nt Le See gc fE mm m mae ie PEL EEe a (EFS EE = Vo Ph We Th Te her Page 126 000588 ornBiomis -Figure 26. Low Standard for PFOSA =J mes ne C ET|R. EE=EE"2 mem= PScttyotdoWiBiSvOi0s7i3n Ble o-- r RR Eee Bei EE a eh apa TEE RAT er EiE nt . poeta SE r IE E -- S= = EE i me oo ERE ee ate ECL TE Sit i] Lats a ii, m Br= e Ee El] HSHfE = Saar CTE BG er 2? 000583 `Northwest Bioanalytical Figure 27. Low Standard for M556 pS oSE eghe om oaSm. eaenbO ]aS .0S wmiEbE eae. RStuedyNNopo..NNoWWBBSRr0O0L-01t6268 fE ee il wp H eSEE hA e TS r L t a mm FE LEI , Shee CEe mmEa Q effC Eb L e re E ea e eww ta CERES Eo -- Ee wnvamoim wemb. e ( FEbaEEAnT,25 pm Ene e nelie fr eiEa h p Eer--talu tle Sa = [occ Tar : EEE Page 125 000590 Northwest Biosnlytical Figure 28. Low Standard for M570 RepSoturdtyNNoo,. NNWWDDRSOOI0--016268 rmreonnt----GE = s= --o _-- m= eb 2 wi=i EeE vex aL B B = e S aJdiE| ae maa = ded a E Gees Bl w e on| =f Sanh Bti ae Eh ---- w= so=eme =3 -- wps=e EeE 22 mem fmm J. wl ESeR l ha Seg r E e-- en t la4RUBEo5 e FP flee SEE Bean le = GRE BE | . : TET BS Ber Page129 000594 A, Figure 29. High Standard for PFOS ome EBoEeeen me WE a ZS SEe EEl EdEP ERRT En =e CR nem RepSorrtyNNooNNWWBOSOR0.O0128L88 E Em sm ei=ih ena B EEa E i h rape s [rc nECEE a : Yen a RRA ey Er -- SpEE= r=EEeEtRIEeEEdI J iE Elan omer fale CERaES a f E ora m --i dr E ee Ob E pe E e or ---- Ada CEES GE] =f 7 : Ye Bh UG Te Page 130 000592 [-- Figure 30. High Standard for PFHS ReSptourdty NNoo,. NNWWBBSROO0L-016286 wep-- o m= Tnnm 5 s mn wxm=b PeE e Em ------ E B Emm m p -- li shroene le hse the t e nites E f ==me m ia a t tlaahcheanen a aE El ma] dBk le io aon l l lb ebeena nena b jE ER [m ienm s-- -- E s= TmS mb " 22 wipPoE 2 [o Emm n lelTe ET ETT EG mle ol Seer pe Swadelies Lag Erm snail femal lAiReE BOE [i onio] l = * =p va = Ve Bh Th Th Te WT Page 131 000593 J---- Figure 31. High Standard for PFOA RSheyNNoop.,NNWoWBBRSrOO0L-0t16265 B mS --eoE n m n) w m p mSp=nyeem E a eI baf [FEoaam JIwL ETE pT wees Emr hae 2 E EmaE ilsaf ni l a fh E Eim smam m aecii] l 0 a Smeo mbl ee ES TR eee (FT -- SEE fFermm Je TT Seesn TemEttEs E for nd e mREa A n LG E EErr mi-- en-- wt onae fhi T a AE a Gl - af WW Bf Th wr Page 132 000594 Northwest Bicoas S te von Study No. NWBS00-128 Figure 32. High Standard for PFOSAA =fTE Zen mBmEEEER mem em famaJa ar ------ feesR Sg En He EE i lial ae EE Ey Lohans -- ow rein" Q2 TEuSenRRR[oEEEi] a2 TREES R eS ames lt fm i aot 7ww ml aEE E E Bm m Em na eefLe en na (RES EA 5 TEES Th Fage 133 000595 Northwest Bioanalytical Figure 33. High Standard for PFOSA RStuedyNNopo..NNWoWBBRSOr0L0--0t16268 o E fIgson m m wEr mnCmr ePEE:E, wo 2 mes 2 me mmm 1wet Le Le E Emme aE r-- r --l a E Ea m ta a E CREE GM ae aan) EAR REIE Te [.-- .. ---- uf A -- a EERE fm amaeJm a eCnotpo y E Elm lm a li hae n Ef mEe a-- e-- l iEa e s PE is eh EE wf Eh THE A fT page 134 000596 Northwest Boalyica! ReSptourdtyNNoo.. NNWWBBSROO0L-016265 Figure 34. High Standard for M556 Elggwees fm E oTnRna. Ea=mE=e f FEao, 4 _ Em mm=me ee Er EE Eee ma e --l t.we i e0 la ef bo E oane Bessie ooJo ol eps Siomfp oanrtiisnnseeisno hn! he peasa i EE IC -- ETTE Rerr ebs -- ery =ay we EEER e --_ =r e poy Sn ime PLATT m a) E offi] nes a E ar et le patched =n. % El = Bh WTS Te wer eens ee 000597 oresSiar Figure 35. High Standard for M570 BE] = = n at Bors. EQr ESSuEen EEnHEa S[EE0E1] Sa2s2 R[ epoNE-- RO 08 bne--ioloChesil aanE|pihf]a EE BEE ah = le Saag GfaelLeaeeehelFosian ER ' eT TREE pwaentee i EEE] --r E--EaE] Eat rr Soild I E-- m-- m el BE [Emmtne] some fe ie Ga = sf aa! EE EY Page 136 00 0598