Document p146pwLJyNmekG0DV9w4yJo6

DownloadSearchable PDFRandom document
June 7, 1961 Memorandum to: Dr. Imbus Dr. Zavon Subject: LEAD DETERMINATIONS FOR jiBB ETHYL CORPORATION In the recent past all the results of the determinations for lead in biologic specimens submitted to the Laboratory from various Ethyl Corporation installations have been routed to Dr. Alvin Davis for appropriate action. The procedure for the disposition of these reports in the future has been reviewed with Dr. Sanders. Dr. Sanders has requested that the reports be handled in the routine fashion by the members of the Clinical Division with the exception that the completed reports should be sent to him for mailing or other disposition. Copies of the reports will be filed as they have been filed in the past. LIM :wp cc: Dr. Kehoe^" Dr. Sanders Dr. Princi Mr. Cholak Mr. Bruegman Lee H. Miller, M.D. KE 0002759 Material : Treatment : Submitted : Required : Samples of feces collected from dogs used in a Lead Alkyl inhalation experiment. Dogs # 6 and 7 are controls. Dog #14 is being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Sample was collected following the 97th period of exposure (7hr./period). Each sample was collected over a three day period dnding 2-13 -6 1. 2-13-61 by Dr. R. K. Davis Concentration of lead in samples of feces Analysis Number 61A-993 61A-994 61A-995 Description 6-2-13-61-26F* 7-2-13-61-26? 14-2-13" 61-1IF Exposed To CONTROL CONTROL TML Weight Grams 10 6 15 2 - 127 Mg. Pb/ Sample 2.90 3-40 1.8 5 Mg. Pb/ 10 0 G. 2.74 2.23 1.46 -First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. f 0002700 Reported: 3-28-61 Material: Samples of feces collected from dogs used in a Lead Alkyl inhalation experiment; Treatment : Dogs # 6 and #7 are controls. Dog #14 is being exposed to the vapors of TML in at a concentration of 0.004 mg. TML per liter of Sample was collected following the 8?th period of exposure (7hr./period). Each sample was collected over a three day period ending 1-50 -6 1. Submitted: 1-50-61 by Dr. R. K. Davis Required: Concentration of Lead in samples of feces. Results Analysis Number Description Exposed To Weight Grams Milligrams Pb/Sample Milligrams Pb/100 gm. 61A-701 6-1-50-61-24F* CONTROL 116 5 .10 2.68 61A-702 61A-705 7-1-50-61-24F 14-1-50-61-18F CONTROL TML 12 0 151 1.6 0 2 .70 1 .33 1.79 *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. K . E 0002761 Material: Treatment : Submitted : Required : Samples of "blood collected from dogs used in a Lead Alkyl inhalation experiment. Dogs #6 and 7 are Controls. Dog #14 is being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Sample was collected following the 92nd period of exposure (7 hr./period). 2-7-61 by Dr. R . K Davis Concentration of Lead in samples of blood Analysis Number 61A-855 6IA-8 5 6 6IA-8 5 7 Description 6-2-6-61-8B* 7- 2- 6-61-8b 14-2-6-61-6b Exposed To CONTROL CONTROL TML Weight Grams 16.5 14.1 17.0 Mg. Pb/ 100 G. 0.014 0.014 0.085 *First number of code is dog number,, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. l/ p 0002762 '' Reported: 4-28-61 Material: Treatment : Submitted : Required : Samples of feces collected from dogs used in a Lead Allqjrl inhalation experiment. Dogs # 6 and 7 are Controls. Dog #14 is being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air Sample -was collected following the 102nd period of exposure (7 hr./period). Each sample was collected over a three day period ending 2-20-61. 2-20-61 by Dr. R. K. Davis Concentration of Lead in samples of feces Analysis Number 61A-1182 61A-1183 61A1184 Description 6- 2- 20-61- 27P * 7-2-20-61-27F 14- 2- 20- 61- 2IF Exposed To CONTROL CONTROL TML Weight Grams 107 10 8 13 6 Mg. Pb/ Sample 1.15 1.10 1.90 Mg. Pb/ 100 G. 1.0 8 1.0 2 1.40 First number of code is dog number, next three numbers is the final date of collection of the sample, and the final numbers are the series of sample and material. 0002763 Reported: 4-27-61 Material: Treatment: Submitted: Required: Samples of blood collected from dogs used in a Lead Alkyl inhalation experiment. Dog # 6 is a control. Dog #14 is being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Sample was drawn following the 107th period of exposure ( 7 hr./period). 3-7-61 by Dr. R. K. Davis Concentration of Lead in samples of blood. Results Analysis Number 6 IA-I669 61A-1670 Description 6-2-24-61-93* 14-2-24-61-73 Exposed To CONTROL TML Weight Grams 19 .2 20 .6 Milligrams Pb/100 g m . 0 .0 21 0 .16 6 *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. Kg' 0002764 Material: Treatment : Submitted: Required: Pooled tissues of four rats which were used in a Lead Alkyl inhalation experiment. These rats died while being exposed to the vapors of TML in air at a concentration of 0.012 m g . TML per liter of air. They received an average of 116 periods of exposure (7 hr./period) each. The first death was after 110 exposures, and the last after 12 0 exposures. > 2 - 6 1 by Dr. R. K. Davis Concentration of Lead in samples of tissues Analysis Humber 61A-1499 6 ^ 15 0 0 61A-1501 61A-1502 61A-1503 61A-1504 Description 328-3-2 -6 1 329->2- 61 330-3-2-61 3 3 I-3- 2- 61 332-3-2-61 333-3-2-61 Tissue BRAIN HEART KIDNEY LIVER LUNG SPLEEN Height Grams 5.2 2.7 8 .8 42.3 5-3 0 .6 Mg. Pb/ Sample 0 .0 5 0.19 0-55 1. 7 8 0.44 0.04 Mg. Pb/ 10 0 G. 1.0 1 6.95 6 .2 6 4.21 8 .26 6 .10 jyj 0002765 Reported: 5-^-6! Material: Treatment: Submitted: Required: Samples of tissues from a dog used in a Lead Alkyl inhalation experiment. Dog #2 received 2 periods of exposure (7 hr./period) to the vapors of TML in air at a concentration of 0.045 mg. TML per liter of air. Twenty days later it received 8 periods of exposure (7 hr./period) to the vapors of TML in air at a concentration of 0.024 mg. TML per liter of air. The dog was sacrificed at final exposure. 8-17-60 by Dr. R. K. Davis Concentration of Lead in samples of tissues Analys is Number 60A-6347 60A-6548 OA-6 3 4 9 60A-6350 OA-6 35I 6 OA-6 3 5 2 oOA-6353 60A-6354 Tissue LIVER SPLEEN LUNG HEART KIDNEY BRAIN FEMUR FAT Weight Grams 232 47 77 62 40 43 32 27 Mg. Pb/ Sample 4.20 0.18 0.22 0.34 0.55 0.27 0.13 0.02 Mg. Pb/ 100 G. 1.8 1 0.39 0.28 0.54 1.39 0.64 0.40 0.06 Q002r'66 Reported: 5-9-61 Material: Treatment: Submitted: Required: Samples of pooled tissues from rats used in a Lead Alkyl inhalation experiment. These rats received 150 periods of exposure (7 hr./ period) to the vapors of TML in air at a concentration of 0.012 mg.'TML per liter of air. 5-6-61 by Dr. R. K. Davis Concentration of Lead in samples of tissues Results Analysis Number 6 lA-l6 l6 6lA-l6l7 6 IA-I6 18 61A-1619 61A-1620 61A-1621 Description 541-5-5-61 542-5-5-61 545-5-3-61 544-5-5-61 545-3-3-61 346-3-3-61 Tissue BRAIN HEART KIDNEY LIVER LUNG SPLEEN Weight Grams Milligrams Pb/Tissue 3.84 0.018 1.70 0.015 5.51 42.88 0.155 0.970 4.07 1.44 ' O.O6 3 0.080 Milligrams Pb/100 gm. 0.47 0.88 2.81 2.26 1.54 5.56 k f 0002767 Reported: 5-25-61 Material: Treatment: Submitted: Required: Samples of pooled tissues of rats used in a Lead Alkyl inhalation experiment. Controls receiving no exposure to lead. 4-17-61 by Dr. R. K. Davis Concentration of lead in samples of tissues Analys is Number 61A-2718 61A-2719 61A-2720 61A-2721 61A-2722 0IA-2 72 3 Description 36 7-4-I2-6I 368-4-12-61 369-4-12-61 370-4-12-61 371-4-12-61 372-4-12-61 Tissue BRAIN HEART KIDNEY LIVER LUNG SPLEEN Weight Grams 8 .8 2 4.23 11.6 0 77*28 6-77 4.01 ` Mg. Pb/ Sample 0.003 0.004 0.016 0.131 0.012 0.005 Mg. Pb/ 100 G. 0.04 0.0 8 0.14 0.17 0.18 0.12 0002768 Reported: 6-1-61 Material : Treatment : Submitted: Required: Samples of pooled tissues of rats used in a Lead Alkyl inhalation experiment. These rats were killed 40 days after 150 periods of exposure (7 hr./period) to the vapors of TML in air at a concentation of 0.012 mg. TML per liter of air. 4-17-61 by Dr. R. K. Davis Concentration of Lead in samples of tissues Analysis Number 61A-2712 61A-2713 61A-2714 61A-2715 61A-2716 61A-2717 Description 361-4-12-61 362-4-12-61 363-4-12-61 364-4-12-61 36 5-4-12 -6 I 366-4-12-61 Tissue BRAIN HEART KIDNEY LIVER LUNG SPLEEN Weight Grams 4.35 2.23 5-19 32.74 3-15 1.66 Mg. Pb/ Sample 0.013 0.007 0.031 0.400 0.015 0.019 ro ro Mg. Pb/ 10 0 G. 0.29 0.32 0 .60 0.47 1.14 /<JE" 0002789 Reported : 6-1-61 Material: Treatment: Submitted: Required: Samples of tissues from a dog used in a Lead Alkyl Inhalation experiment. Dog #14 was sacrificed following 107 periods of exposure ( 7 hr./period), to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. 5 -7 -6 1 by Dr. R. K. Davis Concentration of Lead in samples of tissues Analysis Number 61A-1681 61A-1682 6 IA-I6 8 5 61A-1684 6 IA--I6 8 5 61A-1686 6 IA-I6 8 7 Tissue LIVER SPLEEN LUNG HEART KIDNEY BRAIN FEMUR Weight Grams 154 19 52 59 52 22 40 M g . Pb/ Tissue 2 .8 0 0.09 0.09 0 .28 6.55 0.15 0.19 Mg. Pb/ 100 G. 1.8 1 0.45 0 .18 0.48 1.09 0 .70 0.46 K 0002770 Reported: 6-14-61 Material: Samples of tissues from a dog used in a Lead Alkyl inhalation experiment. Treatment: ; Dog # 6 is a control Submitted: 3-7-61 by Dr. R. K. Davis \ Required: Concentration of Lead in samples of tissues. Analysis Number 61A-1671 61A-1672 61A-1673 61A-1674 61A-1675 6 IA-I6 76 61A-1680 Tissue LIVER SPLEEN LUNG HEART KIDNEY BRAIN BONE Results Weight Grams 19 2 72 48 52 32 25 26 Milligrams Pb/Sample 0.19 0.03 0 .0 2 0 .0 1 0 .0 2 0 .0 1 0.04 Milligrams Pb/100 gm. 0 .10 0.04 0.04 0 .0 2 0.05 0.03 0.17 \ K 0002771 Material: Treatment: Submitted: Required: Samples of the tissues of a dog used in a Lead Alkyl inhalation experiment. Dog #4 received 8 periods of exposure (7 hr./period) to the vapors of TML in air at a concentration of 0.044 mg. TML per liter of air. 2-15-61 by Dr. R. K. Davis Concentration of Lead in samples of tissues. Results Analysis Number 61A-1038 61A-1059 61A-1040 61A-1041 61A-1042 61A-1043 61A-1044 61A-1045 Description Liver Spleen Lung Heart Kidney Brain Femur Fat Weight Grams 258 28 7! 84 51 4l 37 100 Milligrams Pb/Sample 4.64 0.17 0.32 0.55 . O.87 0.33 0.09 0.05 Milligrams Pb/100 ran. 1.95 0 .6 l 0.45 0 .66 1.70 0 .80 0 .2 3* 0 .0 5 *This result was checked by repeat anlysis on the sample solution. 1 0002772 Reported: 4-4-61 Material: Treatment : Submitted: Required: Samples of urine and feces collected from rats used in a Le inhalation experiment. Control rats were not exposed to lead. TML rats are being exposed to the vapors of TML in air at of 0.012 mg. TML per liter of air. Urine sample was collec of glass exposure chamber following exposures #116 through Feces sample was collected under cages during the week in w received exposures #145 to #148. 5-9-61 by Dr. R. K. Davis Concentration of lead in samples of urine and feces Analys is Number 61A-1732 61A-1733 61A-1734 61A-1735 Description 5 5 9-61 554-5-9-61 555-3-9-61 556-5-9-61 Exposed To TML CONTROL TML CONTROL Volume ml. 44 67 URINE Mg. Pb/ Sample 0.16 0.02 Mg. Pb/ Liter 3.70 0 .50 Weight Grams 54.2 32.5 Reported: Material: Treatment: Submitted: Required: Pooled tissues from eight rats used in the Lead Alkyl Inhalation experiments Five male and three female rats were exposed to the vapors of TML in air at a concentration of 0.022 mg. TML per liter of air. All of the rats died "between the 50th and 41st period of exposure (each period of 7 hours duration). 8-1-60 by Dr. R. K. Davis Concentration of Lead in tissues Analysis Number 60A-5935 60A-5936 60A-5937 60A-5938 60A-5939 60A-5940 Description 263-7-27-60-BRAIN 264-7-27-60-HEART 26 5-7-27-60-KIDNEY 266-7-27-60-LIVER 267-7-27-60-LUNG 268 -7-27-6 O-SPLEEN Weight of Sample Grams 6.88 3.33 12.00 42.30 12.20 1.03 Milligrams Pb/Sample 0.07 0.37 0.70 4.50 1.20 0.07 Milligrams Pb/100 G. 1.02 11.0 0 5*83 10.70 9.80 6.90 0002774 Reported: 11-9-60 Materiali Treatment : Submitted: Required: Samples of feces collected from dogs used in the Lead Alkyl Inhalation experiments The exposure of dog #1 consisted of two seven-hour periods, on 7-11 and 12-60, to the vapors of TEL in air at a concentration of 0.068 mg. TEL per liter of air. The exposure of dog #2 consisted of two seven-hour periods, on 7 - H and 12-60, to the vapors of TML in air at a concentration of 0.023 mg. TML per liter of air. Samples from dog #3 were collected following the fifth period of exposure (each of 7 hours duration) to the vapors of TEL in air at a concentration of 0.042 mg TEL per liter of air. ' Samples from dog #4 were collected following the fifth period of exposure (each of 7 hours duration) to the vapors of TML in air at a concentration of 0.044 mg TML per liter of air. Samples from dogs #6,7#10,11,12 and 13 are Control samples . Each sample was collected over a three day period ending 7-26-60. 8-2-60- by Dr. R. K. Davis Concentration of Lead in samples of feces Analys is Number 60A-5964 60A-5965 60A-5966 60A-5967 6 OA- 5968 60A-5969 60A-5972 60A-5973 60A-5974 60A-5975 Description 1-7-27-60-6F* 2- 7-2 7-60-6F 3-7-27-60-3F 4-7- 27- 60-3F 6-7-27-6 0 - IP 7-7-27-60-IP 10-7-2 7-60-IP 11-7-27-60-IP 12-7-27-60-IP 13-7-27-60-IP Weight Grams 123 539 9 90 29 77 40 101 100 12 3 Milligrams Pb /Sample 2.00 8.40 4 .50 4.95 2.8 0 4.83 0.74 2.75 5-00 2.40 Milligrams Pb/100 G. 1.62 I .56 50 .00 5-50 9.70** . 6 .30 ** I .85 2.72 5 .OO** 1.95 *First number of code is dog number, next three is date of final sample collection, and final numbers are the series of sample and material. **Good check were obtained for these results, upon re-analysis of sample r^lution. . Material: Treatment : Submitted : Required : Samples of urine collected from dogs used in the Lead Alkyl Inhalation experiments The controls are dogs 06,7,16 and 17 Dogs 01k and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. The samples were collected following the 2 5th period of exposure (each of 7 hr. duration). Each sample was collected over a three day period ending 10 -3 1-6 0 11-2-60 by Dr. R. K. Davis Concentration of lead in samples of urine Analys is Number 60A-8139 60A-8140 60A-8143 60A-8144 60A-8145 60A-8146 Description 6-IO-3 I-6 O-12U* 7-10-31-60- 12U 14-10-31-60-6U I3-IO-3 I- 60-6U 16-IO-3 I-60-2U I7-IO-3 I-6 O-2U Volume of Sample Milliliters 130 400 500 ; 320 78 0 ** 690 Milligrams Pb/Sample 0.011 0.030 0.203 0.121 0 .0 28 Milligrams Pb/Liter 0.0 8 0.0 8 0.41 0.38 0.04 First number of code is dog number, next three numbers is the date of final sample collection, and the final numbers are the series of sample and material. - Sample lost Reported: II-I5-6 O Material: Treatment Submitted: Required: Samples of urine collected from dogs used in the Lead Alkyl Inhalation experiments. The controls are dogs #6,7*16 and 17. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML/L. of air. Samples were collected following the twentieth exposure period (each of 7 hr. duration). Each sample was collected over a three day period ending 10-24-60. 10-25-60 by Dr; R. K. Davis Concentration of lead in samples of urine. Analysis Number 60A-7956 60A-7957 60A-7960 60A-7961 60A-7962 60A-7965 Description 6-10-24-60-11U* 7-10-24-60-llU 14-10-24-60-5U 15-10-24-60-5U 16 -10 -24 -60 -lU 17 -IO-24 -60 -IU Volume of Sample Milliliters 260 155 410 550 625 635 Milligrams Pb/Sample 0.017 0.019 0.460 0 ol62 0.024 0 .0 29 Milligrams Pb/Liter 0 .0 6 0.13 1.12 0.30 0.04 0.05 First number of code is dog number, next three numbers is the date of final sample collection, and final numbers are the series of sample and material. 0002777 Material: Treatment : Submitted : Required : Samples of feces collected from dogs used in Lead Alkyl Inhalation experiments. Dogs #6 and 7 are CONTROLS Dogs #10 and 11 are being exposed to the vapors of TEL in air at a concentration of 0.012 mg. TEL per liter of air. Samples were collected following the 24th period of exposure (j hr./period). ' Dogs #14 and 15 are pre-exposure. Each sample was collected over a three day period ending 9-26-60. 9-27-60 by Dr. R. K. Davis Concentration of Lead in samples of feces. Analysis Number 6 OA-72 5 8 60A-7259 60A-7262 60A-7263 60A-7264 60A-7265 Description 6-9-26-60 - 7F* 7-9-2 6 - 60 - 7F 10-9-26-60-7F 11-9-26-60-7F 14-9-26- 60- IP 15 -9-26- 60- IF Exposed To CONTROL CONTROL TEL TEL NONE NONE . Weight Grams 316 407 71 50 8 329 213 Mg. Pb/ Sample 3.10 9.80 2.60 6.00 4.50 300 Mg. Pb/ 100 G. 0.98 2.40 3-67 1.18 1.37 1.55 *First number of code is dog number, next three numbers is the final date of sample collection, and final numbers are the series of sample and material Kk 0002778 Reported;:: 1-10-61 Material: Treatment Submitted: Requi.red: Samples of feces collected from dogs used in Lead Alkyl Inhalation experiments. Dogs # 6 and 7 are CONTROLS. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 5th period of exposure (7 hr./period). Each sample was collected over a three day period ending 10-3-60. 10-4-60 by Dr. R. K. Davis Concentration of Lead in samples of feces Analysis Number 60A-7468 60A-7469 0 OA-7 4 7 2 60A-7473 Description 6-10 -3- 60-8f * 7-IO-3 -60-8p 14-10-3- 60- 2F 15 -10-3- 60- 2F Exposed to CONTROL CONTROL TML TML Weight Grams 137 2 72 19 3 ** 346** Mg. Pb/ Sample 6.00 3.30 Mg. P' 100 G 4.37 1.2 1 *FIrst number of code Is dog number, next three numbers is the final date of sample collection, and final numbers are the series of sample and material. **Sample lost. Reported : 1-10-61 Material: Treatment: Submitted: Required: Samples of feces collected from dogs used in Lead Alkyl Inhalation experiments. Dogs # 6 and 7 are CONTROLS* Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0*004 mg. TML per liter of air* Samples were collected following the 10th period of exposure (7 hr./period). Each sample was collected over a three day period ending 10 - 10 -6 0 . 10-11-60 by Dr. R. K. Davis Concentration of Lead in samples of feces Analysis Number 60A-7632 60A-7633 60A-7636 60A-7637 Description 6-10 -10 - 60-9F* 7-10-10- 60-9F 14-10-10- 60-3F 15-10-10-60-3P Exposed To CONTROL CONTROL TML TML Weight Grams 15 8 ** 12 0 ** 88" 206 Mg. Pb/ Sample 1.50 2.50 Mg. Pb/ 10 0 G. 1.70 1.2 1 *First number of code is dog number, next three numbers is the final date of collection of sample, and final numbers are the series of sample and material. **Sample lost. 0002780 Reported: 1-10-61 Material: Treatment : Submitted: Required: Samples of feces collected from dogs used in Lead Alkyl Inhalation- experiments. Dogs #6 and 7 are CONTROLS. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples "were collected following the 15 th period of exposure. (7 hr./period). Each sample was collected over a three day period ending 10-17-60. 10-17-60 by Dr. R. K. Davis Concentration of Lead in samples of feces Analysis Number 60A-7790 60A-7791 60A-7794 60A-7795 Description 6-10-17-60-lQF* 7-10 -17 - 6 0 -1 OP 14-10-17-60-4P 15 -10-17-60-4P Exposed To CONTROL CONTROL TML TML Weight Grams 239 15 1 258 325 Mg. Pb/ Sample 2.40 3.8 0 2.70 3.10 Mg. Pb/ 100 G. 1.0 0 2.32 1.0 5 0 .95 *First number of code is dog number, next three numbers is the final date of collection of sample, and final numbers are the series of sample and material. KE" 0002781 Reported: 1-10-61 Material: Treatment : Submitted. : Required : Samples of feces collected from dogs used in Lead Alkyl Inhalation experiments . Dogs #6 and 7 are CONTROLS. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML/1. of air. Samples were collected following the 20th period of ex posure (7 hr./period). . Each sample was collected over a three day period ending 10-24-60. 10-25-60 by Dr. R. K. Davis Concentration of Lead in samples of feces Analys is Number 60A-7964 60A-7965 60A-7968 60A-7969 Description 6-10-24-60-12F* 7-10-24-60-11F 14-10- 24- 60-5F 15-10-24-60-5F Exposed To CONTROL CONTROL TML TML We ight Grams 153 135 548 412 Mg. Pb/ Sample 5.20 1.40 5 60 7.8 0 Mg. Pb/ 10 0 G. 2.09 1.04 1.04 1.89 First number of code is dog number, next three numbers is the final date of collection of sample, and final numbers ard the series of sample and material g g 0002782 Reported: 1-15-61 Material: Treatment : Submitted: Required: Pooled tissues of 3 male and 2 female rats used in Lead Alkyl Inhalation experiments, (sections of the tissues were removed for micropathology). These are Control rats . They were killed 39 days after 13 0 periods of exposure (7 hr./period) to 32 liters per minute of room air plus 0.1 liters per minute of dry nitrogen. 11-17-60 by Dr. R. K. Davis Concentration of Lead in tissues. Analysis Number 60A-8541 60A-B5^-2 60A-8543 6 OA-8 5 4 4 60A-8545 60A-8546 Description 30 8 -II-I7--60 309-11-17-60 3 IO-II-I7-0 O 311-11-17-60 312-11-17-60 313-11-17-60 Tissue BRAIN HEART KIDNEY LIVER LUNG SPLEEN Weight Grams 7.7 4.7 10.6 12 .3 6 .7 2.4 Mg. Pb/ Sample 0.0021 0.0013 0.0214 0.0333 0.0020 0.0020 Mg. Pb/ 100 G. 0.027 0.027 0.202 0.266 0.030 0.083 /<- 0002783 Reported : 1-24-61 Material: Treatment : Submitted : Required : Samples of feces collected from dogs used in Lead Alkyl Inhalation experiments. Dogs #6 and 7 are CONTROLS. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 30th period of exposure (7 hr./period). Each sample was collected over a three day period ending 11-7-6 0 . 11-8-60 by Dr. R. K. Davis Concentration of Lead in samples of feces . Analysis Number 60A-8286 6 OA-8 2 8 7 6 OA-8 2 9 0 60A-8291 Description 6- 1 1 - 7- 60 - 13 F* 7-11- 7-60-13F 14-11- 7- 60-7F I5-II- 7-60-7F Exposed To CONTROL CONTROL TML TML Weight Grams 89 103 88 195 Mg. Pb/ Sample 2.90 2.30 2.20 2.30 Mg. PI 100 G 3 *26 2.23 2.50 1.18 *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and matera -Kg' 0002784 Reported: 1-24-61 Material: Treatment : Submitted: Required: Samples of urine collected from dogs used in Lead Alkyl experiments . Dogs #6 and 7 are CONTROIS. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 59th period of exposure (7 hr./period). Each sample was collected oyer a three day period ending 12-19-60. 12-19-60 by Dr. R. K. Davis Concentration of Lead in samples of urine Analysis Number 60A-9376 60A-9377 6 OA-9 3 8 0 60A-9381 Description 6-12-19-60-19U* 7-12-19-60-19U 14-12-19-60-13U 15-12-19-60-13TJ Exposed To CONTROL CONTROL TML TML Volume ml. 190 200 225 650 Mg. Pb/ Sample 0.019 0.028 o.4oo 0.300 Mg. Pb/ Liter 0.10 0.14 1.77 0.46 *Eirst number of code is dog number, next three numbers is the final date of collection of sample, and final numbers are the series of sample and material 0002785 Reported: 1-26-61 Material: Treatment: Submitted: Required: Samples of feces collected from dogs used in Lead Alkyl experiments . Dogs # 6 and 7 ard CONTROLS. Dogs $14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples -were collected following the 3 5 th period of exposure (7 hr./period). Each sample was collected over a three day period ending 11-14-60. II-I5-6 0 by Dr. R. K. Davis Concentration of Lead in samples of feces . Analysis Number 60A-8456 60A-8457 60A-8460 60A-8461 Description 6-11-14- 60-14F* 7-11-14-60-14F 14-11-14- 60-8P 15-1M4-60-8F Exposed To CONTROL CONTROL TML TML Weight Grams 166 113 12 1 267 0 VO 1--l Mg. Pb/ Sample 2.8 0 1.30 3-10 Mg. P 100 G 1.69 1.15 1.52 1.16 -*First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. 0002786 Reported: 1-26-61 Material : Treatment : Submitted : Required : Sample of room air collected in 95% Ethyl Alcohol 3 6 M3 of room air was scrubbed with brominated 95% Ethyl Alcohol, over a period of 26 days. 1-23-61 by D r . R . K . Davis Concentration of Lead and Nickel in sample of room air. Analysis Number 61A-547 Description 213-1-19-61 Volume of Sample _______ Mf_______ 3-6 Micrograms Ni/M3 3 .4 Micrograms Pb/M3 5-7 riq i-v :O i Reported: 1-27-61 Material: Treatment : Submitted : Required ; Samples of urine collected from dogs used in Lead Alkyl experiments . Dogs #6 and 7 are CONTROLS . Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 72nd period of exposure (7 hr./period). " ' Each sample was collected over a three day period ending 1- 9-61. 1-10-61 by Dr. R. K. Davis Concentration of Lead in Samples of urine Analysis Number 61A- 20 5 SIA-206 61A-209 SIA-210 Description 6- 1-9- 6I-2 IU* 7-1-9- 61- 21U 14-1-9-S1-15U 15-1-9-61-15U Exposed To CONTROL CONTROL TML 3EML Volume ml. 290 285 190 3 10 Mg. Pb/ Sample 0.023 0.022 0.410 0.420 Mg. Pb Liter 0.0 8 0.0 8 2.16 1.36 EJpst number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. 0002788 Reported! 1-27-61 Material: Treatment: Submitted: Required: Samples of urine collected from dogs used in Lead Alkyl experiments Dogs #6 and #7 are controls. Dogs #14 and #15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 64th period of exposure (7 hr./period). Each sample was collected over a three day period ending 12 -26 -6 0 . 12-27-60 by Dr. R. K. Davis Concentration of Lead in samples of urine Analysis Number 60A-9566 60A-9567 60A-9570 60A-9571 Description 6-12-26-60-20U* 7-12-26-60-20U 14-12-26-60-14U 15-12-26-60-14U Results Exposed To Volume ml. CONTROL 5 10 CONTROL 200 TML 200 TML 750 Milligrams Pb/Sample 0 .0 25 0.040 0.121 1.520 Milligrams Pb/Liter 0.07 0.20 0 .60 1.76 First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. K? 0002789 V d r. R. A. Kehoe Library To: Date : 6/29/59 Name of Report and Date: SUMMARY OF PRELIMINARY TOXICOLOGIC INVESTIGATION OF T HE LEAD ALKYLS dated June.!#, 1959 (Ethyl Corporation)______________________ No. of Copies of Report Made: 6l_______ No. of Copies of Report (Bound) with Figures: ___________ No. of Copies of Report (Bound) without figures: ____ 0 No. of Copies of Report (Unbound) with Figures: _________ No. of Copies of Report (Unbound) without Figures: ____ 1 Distribution: ___ 1 26 1 Unbound - Dr. Kehoe Bound - Dr, Kehoe ) H 6/26/59 ) Bound - Dr. Brust______ 6/26/59 33 C Bound - Dr, Kehoe_____ 6/30/59 (for further ry to r i* 1' distribution i v Aa - L71\-i n ^ - o y<nk j ' No. of Copies placed in Library: Remarks : __ f.{! -f- Signature : \ - h u g t , Jlj.j) Albert A Brust, M.D. KF' 0002700 Material: Treatment : -Submitted : Required: Samples of urine and feces collected from rats used in Lead CONTROL rats were not exposed to lead. TML rats are being exposed to the vapors of TML in air at a mg. TML per liter of air. Urine sample was collected, from exposure chamber, following the 8 7th through 115 th'period o Feces samples were collected, from paper placed under the c 111th through 114th period of exposure (7 hr./period). 1-17-61 by Dr. R.'K. Davis Concentration of lead in samples of urine and feces. Analysis Number 61A-43J 61A-438 61A-439 61A-440 Description 534-1-16-61 335-1-16-61 336-1-16 -6 1 337-1-16-61 Exposed To TML CONTROL TML CONTROL Volume ml. 44- 115 URINE Mg. Pb/ Sample 0 .13 0 0.04-8 Mg. Pb/ Liter 2.96 0.41 Weight Grams 23.5 2 7 .O Reported: Material: Treatment: Submitted: Required: Analysis Number 6IA-7 14 Sample of blood collected from a dog used in Lead Alkyl Inhalation studies. - This dog was being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Sample was taken at sacrifice after 8 7 periods of exposure (7hr./period). 1-30-61 by Dr. R. K. Davis Concentration of Lead in sample of blood. Results Description 15-1-30-61-6B* Weight Grams 18.4 Milligrams Pb/100 Grams 0 .1 2 *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. 0002702 Reported: 2-6-61 Materiali Treatment : Submitted : Required : Samples of feces collected from dogs used in Lead Alkyl inhalation experiments. _ Dogs # 6 and 7 are Controls. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 59 th period of exposure (7 hr-./period) Each sample was collected over a three day period ending 11- 2 1- 6 0 . 11-21-60 by Dr. R. K. Davis Concentration of Lead in samples of feces Analysis Number 60A-8647 60A-S648 60A-8651 SOA-8 6 5 2 Description 6- 11- 2 1-60-I5F* 7-II-2 I-0 O-I5F 14-11- 2 1- 60-9? I5-II- 21-60-9? Exposed To CONTROL CONTROL TML TML Weight Grams 15 8 16 3 137 233 Mg. Pb/ Sample 1.70 0.94 I .85 2.40 Mg. Pi 10 0 rz 1.0 8 O .58 1.35 1.03 *First number of code is dog number, next three numbers is the final date of collection of sample, and final numbers are the series of sample and material. K E 0002 7 D3 Reported ; 2-9- 61 Material: Samples of blood collected from dogs 4sed in a Lead Alkyl Inhalation experiment. __ Treatment: Dogs # 6 and #7 are controls. Dogs #14 and #15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 72 nd period of exposure ( 7 hr./period). Submitted: 1-9-61 by Dr. R. K. Davis Required: Concentration of Lead in samples of blood. Results Analysis Number Description Exposed To Weight Grams Milligrams Pb/100 gm. 61A-184 6-1-9-61-7B* CONTROL 17.0 0 .0 10 6 IA-18 5 7-1-9-61-7B CONTROL 16.5 0 .0 20 61A-188 6 IA-I89 14-1-9-61-5B 15 -I-9 -6 1-5B TML TML 15.4 15.3 0.075 0.085 First number of code is dog number, next three numbers is the final date of collection of sample, and final numbers are the series of sample and material. 0002794 Material: Treatment : Submitted: Required: Samples of blood collected from dogs used in a Lead Alkyl inhalation experiment. Dogs #5 and 7 are Controls. Dogs rl14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 rag. TML per liter of air. Samples were collected following the 54 period of exposure (7 ,hr./period). * 12-15-60 by Dr. R. K. Davis . Concentration of lead in samples of blood Analysis Iiumber oOA-9232 SOA-9233 60A-9236 6 OA-9 2 3 7 Description b-12 -12 -- 60-6B* 7- 12 -12 - 60-6b 14-12 -12 -60-4b lp--12--L2-- 0 0--4B Exposed To CONTROL CONTROL TML TML Weight Grams 19.2 . 23.9 10.4 ' 10.7 Mg. Pb/ 100 G . 0 .0 15 0 .0 10 O.O65 0.045 *Rirst number of code is dog number next three numbers is the final da of collection of sample, and final numbers is the series of sample and material. * K 0002795 Material: Treatment: Submitted: Required: Samples of feces collected from dogs used in a Lead Alkyl Inhalation experiment Dogs # 6 and #7 are controls. Dogs #14 and #15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 44th period of exposure (7 hr./period). Each sample was collected over a three day period ending 1 1 -28 -6 0 . 11-28-60 by Dr. R. K. Davis Concentration of Lead in samples of feces. Results Analysis Number 60A-8829 6 OA-8 8 3O 60A-8833 60A-8834 Description 6 -II-28 -6 O-I6F* 7 - H - 28 -6O-I6F 14-11-28-60-10F I5-II-28 -60 -IOF Exposed To CONTROL CONTROL TML TML . Weight Grams 15 0 153 157 223 Milligrams Pb/Sample 1.65 2.40 3 .10 3.8 0 Milligrams Pb/100 gm. 1.10 1.57 1.97 1.70 First number of code is dog number, next three numbers is the final date of collection of sample, and final numbers are the series of sample and Material. 0002706 Material: Treatment : Submitted: Required : Samples of feces collected from dogs used in an Lead Alkyl inhalation experiment - Dogs # 6 and 7 are Controls. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 49th period of exposure (7 hr./period). Each sample was collected over a three day period ending 12 -5-cO. 12-6-60 hy Dr* R. K. Davis Concentration of lead in samples of feces Analysis Humber 60A-8975 60A-8976 60A-8979 o OA-8980 Description 6-12 -5-60-1?* 7-12-5-60-17F 14-12-5-60-llF I5- I 2- 5- 6 0 -llF Exposed To CONTROL SONTROL TML TML Weight Grams 12 1 15 1 15 8 228 Mg. Pb/ Sample 1.40 1.10 I .50 2 .40 Mg. Pb/ 10 0 G. 1.16 0.75 0.95 1.0 5 *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. 0002707 Reported: 2-l^-6l Material : Treatment : Submitted: Required : Samples of urine collected from dogs used in a Lead Alkyl inhalation experiment Dogs # 6 and 7 are Controls. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 77th period of exposure (7 hr./period). Each sample was collected over a three day peri od ending 1-16-61. . I-I7- 6I by Dr. R. K. Davis . Concentratiion of lead in samples of urine. Analysis Numb er 6 IA-4 2 5 61A-425 61A-427 61A-428 Description 6-1-16-S1-22U* 7-1-16- 61- 22U 14-1-15- 61- 16U I5-I-I6- 6 I-I6U Exposed To CONTROL CONTROL TML TML Volume ml. 135 225 , I8 5 230 Mg. Pb/ Sample 0.017 0 .0 20 0-330 0.354 Mg. Pb/ Liter 0 .12 0.09 1. 7 8 1.54 First number of code is dog number, next three numbers is the final date of collection of sample, and final numbers are the series of sample and material. KP 0002708 Reported: 2-10-61 Material: Treatment : Submitted: Required : Samples of urine collected from dogs used in a Lead Alkyl inhalation experiment. Dogs #6 and 7 are Controls . Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples -were collected following the 8 2nd period of exposure (? hr./period). Each sample was collected over a three day period ending 1- 25-6 1. 1-25-61 by D r . R . K . Davis Concentration of lead in samples of urine Analysis Rumber 61A-559 6IA-5 SO 61A-56 I SIA-562 Description 6-1-25-61- 25-U* 7-1-25-61- 25U 14-1-25-61-1HJ 15 - 1- 25-61-17U Exposed To CONTROL CONTROL TML TML Volume ml. 18 0 195 155 540 Mg. Pb/ Samole 0.05 0 .02 0.41 0 .41 Mg. PI Liter 0 .16 0 .12 2 .6 6 1.2 1 *First number of code is dog number* next three numbers is the final date of collection of sample* and final nmmbers are the series of sample and material. K'g' 0002799 Reported: 2-14-61 Material : Treatment : Submitted: Required : Samples of tissues from a dog used ina. Lead Alkyl inhalation experiment. Dog #10 received 29 periods of exposure (7 hr./period) to the vapors of TELin air at a concentration of 0.012 m g . TEL per liter of air 1-16-61 by Dr* R. K. Davis . Concentration of Lead in samples of tissues Analysis Number 6 IA-353 61A-3 54 6IA-3 6 5 61A-3 66 61A-367 61A-368 olA-369 Description Liver Spleen Lung Heart Kidney Brain Bone . Weight Grams 156 11 48 47 24 84 2 1.5 Mg. Pb/ Sample 6.02 0.09 0 .0 6 0.03 0.25 0.15 0.39 Mg. Pb/ 100 G. 3.86 O .85 0 .14 0 .06 1.0 5 0 .18 1.8 1 0 0 02 S 0 -Reported : 2-14-61 Material: Treatment : Submitted : Requiredi Samples of urine collected from dogs used in a Lead Alkyl inhalation experiment. Dogs # 6 and 7 aee Controls. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Sample from dog #14 was collected following the 8 7th period of exposure (7 hr./period). Sample from dog # 15 was removed from the bladder at sacrifice after 8 5 periods of exposure (1-24-61) Each sample (except dog #15) was collected over a three day period ending 1-30 -6 1 . 1-30-61 by Dr. R. K. Davis Concentration of lead in samples of urine Analysis Humber SIA-70S S I A- 7 09 S I A - 710 S I A-7 11 Description 6 -1-3 0 -6 1- 24U* 7 - 1- 3 0 - 6 1- 24U 14 - 1- 3 0 - 6 1- 18 a I 5 - I - 3 0 - 6 1- 18 U Exposed To CONTROL CONTROL TML TML Volume ml. 13 0 14 5 295 80 Mg. Pb/ Sample 0 .0 2 0 .0 2 0 .6 2 0 .2 9 Mg. Pb/ Liter 0 .17 0 .13 2 .10 3 .5 7 *First number of code is dog number, next three numbers is the final date of collection of sample, and final numbers are the series of sample and material 0 00 2301 Material: Treatment : Submitted-: Required: Samples of feces collected from dogs used in a Lead Alkyl inhalation experiment. Dogs # 6 and 7 are Controls . Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 54th period of exposure (7 hr./period). Each sample was collected over a three day period ending 12 -12 - 6 0 . 12-13-60 by D r . R . K. Davis Concentration of lead in samples of feces Analysis Humber oOA-9247 60A-9243 0QA-9 2 5I 60A-9252 Description 5-12-12- 60-18 P* 7-12-12-60-18F 14-12-12-60-12F I5-I2-I2-60-12 F Exposed To CONTROL CONTROL TML TML CC?-O\ Weight Crams 10 8 lpp 161 Mg. Pb/ Sample 1.10 1.3 0 2.50 4.50 Mg. Pb/ 100 G. 1.0 2 0.98 3 68 2 .80 *First number of code is dog number, next three numbers is the final date of collection of sample, and final numbers are the series of sample and material. Kfi 0002?C2 Material : Treatment Submitted Required Samples of tissues from a dog used in a Lead Alkyl inhalation experiment Dog #11 received 24 periods of exposure (7 hr./period) to the vapors of E L in air at a concentration of 0 .0 12 mg. TEL per liter of air. 1-16-61 Toy Dr < R. K. Davis Concentration of lead in samples of tissues Analysis Number 6IA-3 7 O SIA-3 71 CIA-372 61A-373 51A-374 SlA-375 SIA-3 75 Description Liver Spleen Lung Heart Kidney Brain Bone Height Grams 443 26 75 77 57 So 34 Mg. PL/ Sample 9.54 0.15 0 .20 0.13 0.31 0.17 O .52 Mg. Pb/ 10 0 G. 2.13 0.59 0 .26 0 .17 0 .55 0.29 1.54 R B 0002503 Material: Treatment : Submitted: Required: Samples of feces collected from dogs used in a Lead Alkyl inhalation experiment. Dogs #6 and #7 are controls. Dogs #14 and #15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 59th period of exposure (7 hr./period). Each sample was collected over a three day period ending 12 -19 -6 0 ; 12-19-60 by Dr. R. K. Davis Concentration of Lead in samples of feces. Results Analysis Number 60A-9370 60A-9371 60A-9374 60A-9375 Description 6-12-19-60-19F* 7-12-19-60-19F 14-12-19-60-13F 15 -12 -19 -60 -13 F Exposed To CONTROL CONTROL TML TML Weight Grams 91 89 / 10 8 235 Milligrams Pb/Sample 2.20 1.10 2.40 7.80 Milligram: Pb/100 gm 2.42 1.24 2.22 3.32 First number of code is dog number, next three numbers is the final date of collection of sample, and final numbers are the series of sample and material. HZ 0002SC4 Reported: 2-17-61 Material: Treatment: Submitted: Required: Samples of tissues from a dog used in a Lead Alkyl inhalation experiment. Dog #12 received 15 periods of exposure ( 7 hr./period) to the vapors of TML in air at a concentration of 0.012 mg. TML per liter of air. 1-16-61 by Dr. R. K. Davis Concentration of Lead in samples of tissues. Results Analysis Number 61A-380 6 IA-38 I 61A-382 6IA-3 8 3 61A-384 61A-385 Description Liver Spleen Lung Heart Kidney Brain Weight Grams 128 11 51 44 24 49,5 Milligrams Pb/Sample 2.03 0.05 0.12 ; 0 .18 0 .18 0 .31 Milligrams Pb/100 gm. 1.59 0.51 0.24 0.41 0.7 6 O .63 0002805 Material: Treatment : Submitted: Required: Samples of feces collected from dogs used in a Lead Alkyl inhalation experiment Dogs # 6 and 7 are Controls. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. samples were collected following the 72 nd period of exposure (7 hr./period). Each sample was collected over a three day period ending 1-9-61. 1-10-61 by Dr. R. K. Davis Concentration of Lead in samples of feces Analysis Humber 61A-211 61A-212 61A-215 6 1A-2 16 Description 6-1-9-61-21F* 7-1-9-61-21F 14-1-9- 61-15 F I5-I-9-6 1-15 F Exposed To CONTROL CONTROL TML TML Height Grams 12 2 165 127 217 Mg. Pb/ Sample 3 .10 4.20 1.90 2.60 Mg. PI 100 G 2.54 2.54 1.5 0 1.2 0 *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and matrial. 0002S06 Material: Treatment: Submitted: Required: Samples of tissues from a dog used in a Lead Alkyl inhalation experiment. Dog #3 received 7 periods of exposure (7 hr./period) to the vapors of TEL in air at a concentration of 0.042 mg. TEL per liter of air. 2-15-61 by Dr. R. K. Davis Concentration of Lead in samples of tissues Analysis Number 61A-1031 61A-1032 61A-1053 6IA-IO34 61A-1035 61A-1036 61A-1037 Description LIVER SPLEEN LUNG HEART KIDNEY BRAIN FEMUR 0 =j- 00 Results Weight Grams 337. 23.8 100. 58. 38. 36. Milligrams Pb/Sample 15 .9 0 0.11 0.21 0.15 . 0.85 0.10 0.43 Milligrams Pb/100 gm 4.73 0.46 0.21 0.17 1,47 O .2 5 1.20 H E 0002 807 Reported: 3**10-6l Material: Treatment: Submitted: Required: Samples of urine collected from dogs used in a Lead Alkyl inhalation experiment Dogs # 6 and 7 are Controls Dogs #14 is being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 97th period of exposure (7 hr./period). Each sample was collected over a three day period ending 2-15 -6 1 . 2-15-61 by Dr. R. K. Davis Concentration of lead in samples of urine Analysis Number 61A-988 6 1A-989 61A-990 Description 6-2-15 -6 1-26U* 7- 2-15 -6 1-26U 14-2-15 -6 1-2 OU Exposed To CONTROL CONTROL TML Volume ml. I8 5 260 395 M g . Pb/ Sample 0.05 0.04 0.87 M g . Pb Liter 0.18 0.16 2 .2 1 *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. K 1= 0 0 0 2 8 0 8 Reported: 5-14-61 Material : Treatment : Submitted : Required : Samples of feces collected from dogs used in a Lead Alkyl inhalation experiment. Dogs #6 and 7 are controls Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 77th period of exposure (7 hr./period). Eaeh sample was collected over a three day period ending 1-16-61 1-17-61 by Dr. R. K. Davis Concentration of lead insamples of feces Analysis Number 61A-431 61A-432 61A-433 61A-434 Description 6-1-16-61-22F* 7-1-16-61-22F 14-1-16-61-16? I5-I-I6- 6 1-16 ? Exposed To CONTROL CONTROL TML TML Weight Grams 82 12 5 122 113 Mg. Fb/ Sample 0 .82 1.90 1.6 0 1.40 CM H Mg. Fb/ 100 g. 1.0 0 1.5 2 1.3 1 *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. ^ 0002809 Reported: 3"14-61 Material: Treatment: Submitted: Required: Samples of urine collected from dogs used in a Lead Alkyl inhalation experiment. Dogs #6 and #7 are controls. Dog #14 is being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Sample was collected following the 92nd period of exposure (7 hr./period). Bach sample was collected over a three day period ending 2 -6 -6 1 . 2-8-61 by Dr. R. K. Davis Concentration of Lead in samples of urine. Results Analysis Number 61A-888 6IA-8 8 9 61A-890 Description 6-2-6-61-25U* 7-2-6-61-25U 14-2-6-61-19U Exposed To CONTROL CONTROL TML Volume ml. 170 170 l4o Milligrams Pb/Sample 0.03 0.03 0.34 Milligrams Pb/Liter 0 .18 0.19 2.42 *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. K ^ 0 0 0 2 8 10 Material: Treatment: Submitted: Required: Samples of tissues collected from a dog used in a Lead Alkyl inhalation experiment. Dos #5 received 30 periods of exposure (7 hr./period) to the vapors of TEL in air at a concentration of 0.022 mg. TEL per liter of air. 1-16-61 by Dr,. R. K. Davis Concentration of Lead in samples of tissues Results Analysis Number 61A-357 61A-358 61A-359 6 IA-3 6 0 6 IA-3 6 1 6 lA- 362 Description Liver Spleen Lung Heart Kidney Brain* Weight Grams 387 30 120 95 65 Milligrams Pb/Sample 9.14 1.68 2.92 1.92 1.55 Milligrams Pb/100 gm; 2.36 5.6 0 2.43 2.02 2.39 *No sample received. K Z 000281 i Material: Treatment: Submitted: Required: Samples of feces collected from dogs used in a Lead Alkyl inhalation experiment. Dogs #6 and 7 are Controls. Dogs #14 and 15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 64th period of expoaure (7 hr./period). Each sample was collected over a three day period ending 12-26-60. 12-27-60 by Dr. R. K. Davis Concentration of Lead in samples of feces Analysis Number 60A-9572 60A-9573 oOA-9576 60A-9577 Description 6-12-26-60-2CF* 7-12 -26- 6 0 -2 OF 14-12- 26-60- 14F 15-12-26-60-14F Exposed To CONTROL CONTROL TML !TML Weight Grams 96 122 120 241 Mg. Pb/ Sample 1.60 2.10 2.10 5-00 Mg. Pb/ 100 G. 1.67 1. 7 2 1*75 2.0 8 *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. KT 0002812 Reported: >*8-61 Material: Treatment: Submitted: Required: Samples of urine collected from dogs used in a Lead Alkyl inhalation experiment. Dogs #6 and #7 are controls. Dog #14 is being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air; Sample was collected following the 102nd period of exposure (7 Hr./period). Each sample was collected over a three day period ending 2-20 -6 1. 2-20-61 by Dr. R. K. Davis Concentration of Lead in samples of urine. Results Analysis Number 61A-1177 61A-1178 61A-1179 Description 6-2-20-61-27U* 7-2-20-61-27U 14-2-20-61-21U Exposed To CONTROL CONTROL TML Volume ml. 160 185 350 Milligrams Pb/Sample 0.03 0.10 0.05 Milligrams Pb/Liter 0.20 0.54** 0.14** *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. **These results were checked by a repeat analysis of sample. j<?J= 0002813 Reported: 5-15-61 Material: Treatment : Submitted: Required: Samples of urine collected from dogs used in an AP-Tl inhalation experiment Dogs # 6 and 7 are Controls Dogs #16 and 17 are being exposed to the vapors of AP-Tl in air at a concentration of 0*004 mg. AP-Tl per liter of air. Samples of urine were collected following the 30 th period of exposure (7 hr./period). 2-20-61 by Dr. R. K. Davis Concentration of Nickel in samples of urine Analysis Number 61A-1177 6IA-II78 61A-1180 61A-1181 Description 6-2- 20-6 1- 27U* 7~2- 20 -61- 27U 16-2-20- 61-12U I7- 2-2 I-61-12U Exposed To CONTROL CONTROL AP-Tl AP-Tl Volume ml 0 I60 ** I8 5 490 320 Micrograms N /Sample - 47 84 740 Micrograms Ni/Liter - 254 I7 I*** 2310 *First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sampleand material. **Sample lost in process of analysis ***Result checked by repeat analyses of same sample K4 0002814 Reported: > "15" 61 Material: Treatment: Submitted: Required: Samples of feces collected from dogs used in a Lead Alkyl inhalation experiment. . Dogs #6 and #7 are controls. Dogs #14 and #15 are being exposed to the vapors of TML in air at a concentration of 0.004 mg. TML per liter of air. Samples were collected following the 8 2nd period of exposure (7 hr./period). Each sample was collected over a three day period ending 1-2 3 -6 1. 1-23-61 by Dr. R. K. Davis Concentration of Lead in samples of feces. Results Analysis Number 61A-565 6IA-5 6 6 6IA-5 6 7 6IA-5 6 8 Description 6-1-23-61-23F* 7-1-23-61-23F 14-1-23-61-17F 15-1-23-61-17F Exposed To CONTROL CONTROL TML TML Weight Grams 116 84 90 125 Milligrams Pb/Sample 1.00 1.30 1.70 2.10 Milligrams Pb/100 gm. 0.86 1.55 1.8 9 1.68 *First number of code Is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. j / g 0002815 Reported: 3 -1 5 - 6 1 Material: Treatment: Submitted: Required: Samples of tissues of a dog used in a Lead Alkyl inhalation experiment. Dog #15 received 14 periods of exposure ( 7 hr./Period) to the vapors of TML in air at a concentration of . 0.012 mg. TML per.liter of air. 1-16-61 by Dr. R. K. Davis Concentration of Lead in samples of tissues Analysis Number 61A-388 61A-389 61A-3 90 61A-391 61A-392 61A-393 61A- 3 94 Description Liver Spleen Lung Heart Kidney Brain Spinal Cord Weight Grams 270 15 242 77 48 72 14 Mg. Pb/ Sample 3.60 0.06 O .33 0.29 O.5 I 0.79 0 .0 5 Mg .Pb/ 100 G. 1.34 0.41 0.14 0.38 I.O8 1.09 0.39 t< 0 0 0 2 8 16 Reported 2-24-61 Material: Treatment : Submitted: Required: Samples of feces collected from dogs used in a Lead Alkyl inhalation experiment. ' Dogs # 6 and #7 are controls. Dog #14 is being exposed to the vapors of TML in air at a concentration of 0,004 mg. TML per liter of air. Sample was collected following the 92nd period of exposure ( 7 hr./period). Each sample was collected over a three day period ending 2-6-6 1. 2-8-61 by Dr. R. K. Davis Concentration of Lead in samples of feces. Results Analysis Number 61A-893 61A-894 61A-895 Description 6-2-6-61-25F* 7-2-6-61-25F 14-2-6-61-19F Exposed To CONTROL CONTROL TML Weight Grams 98 116 56 Milligrams Pb/sample 1.90 2 .70 1.30 Milligrams Pb/lOO gm. 1.94 2.33 2.32 First number of code is dog number, next three numbers is the final date of collection of the sample, and final numbers are the series of sample and material. 0002817 Reported: 3**29-6l Material: Treatment: Submitted: Required: Samples of pooled tissues from four rats used in a Lead Alkyl inhalation experiment. These rats received 150 periods of exposure (7 hr./period) to the vapors of TEL in air at a concentration of 0.012 mg. TEL per liter of air. They were sacrificed 39 days after final exposure. 11-17"60 by Dr. R. K. Davis Concentration of Lead in samples of tissues. Results Analysis Number 60A-8535 60A-8536 60A-8537 6OA-8 538 0OA-8 5 3 9 60A-8540 Description 30 2-II-I7 -6O 30 3- H - I 7 -6O 304-11-17-60 30 5- H - I 7 -6O 30 6 -II-17 -6 O 3 0 7-II-I7 -6 O Tissue BRAIN Heart Kidney Liver Lung Spleen CM O Weight Grams 5.44 7.55 49.80 4.76 1.55 Milligrams Pb/Sample 0 .0 10 0 .0 0 6 0.041 0.300 0 .0 0 6 0 .0 0 6 Milligrams Pb/100 gm. 0 .18 0 .18 0.54 0 .60 0.13 0.95 0 0 0 2 8 i8 Material: Samples of urine and blood from dogs used in the Lead Alky Treatment: Exposure to room air with no added Lead was started 8-22-6 Exposure to TEL vapors at a concentration of 0.012 mg; TEL started 8 -2 2 -6 0 for dogs #10 and #11. pre-exposure samples were taken on dogs #14 and # 1 5 ; Urines were collected over a three day period ending 9-26- All days are exposed seven hours every day except Saturday Submitted: 9-27-60 by Dr. R. K. Davis Required: Concentration of Lead on samples from all dogs except #8 a Manganese on samples from dogs #6,#7,#8 and #9 . Analysis Number Description Volume Milliliters URINE Milligrams Pb/Sample Milligrams Pb/Liter We Gr 60A-7246 60A-7247 60A-7250 6 -9-26 -6O-7U* 7 -9-26 -6O-7U 10-9-26-60-7U 522 413 74 0.035 0 .0 50 0 .228 0 .11 0 .12 5.07 60A-7251 11-9-26-60-7U 513 0.995 1.94 60A-7252 14-9-26-60-lU 336 0.031 0.09 60A-7253 60A-7254 60A-7255 60A-7256 60A-7257 15 -9 -26 -60 -lU 10 -9 -26 -60 -te 11-9-26-60-4B 14-9-26-60-lB I5 -9 -26 -6O-IB 220 .- - mm - 0.024 - mm 0 .11 - mm M - 1 1 *pirst number of code is dog number, next three numbers is the date of final sa and final numbers are the series of sample and material. Material: Treatment: Submitted: Required: A sample of blood from a dog used in the Lead Alkyl Inhalation experiments. This dog was exposed 15 times (7 hr./exposure) to the vapors of TML at a concentration of . 0.011 mg. TML/L. of air. 9-12-60 by Dr. R. K. Davis Concentration of Lead in blood; Analysis Number 60A-6871 Description 12-9-12-60-4B* Weight of Sample Grams 8.9 Milligrams Pb/100 gms. 0 .0 85 First number of code is dog number, next three numbers is the date of final sample collection, and final numbers are the series of sample and material. Material: Treatment : Submitted: Required: Samples of feces from rats used in the Lead Alkyl Inhalation experiments; TEL-rats were exposed 7^ times (7 hr./exposure) to TEL vapors at a concentration of 0.012 mg. TEL/L. of air. TML-rats were exposed 2k times (7 hr./exposure) to TML vapors at a concentration of 0.022 mg. TML/L. of air. CONTROL-rats were exposed 7^ times (7 hr./exposure) to room air plus 0.3$ dry nitrogen, with no added lead. Peces were collected on paper placed under the cages. 6-21-60 by Dr. R. K. Davis Concentration of lead in feces samples Analysis Number 60A-5081 60A-5082 60A-5083 Description 236 -6 -I7 -6 O-TEL 2 3 7-6 -17 -6 O-TML 238 -6 -I7 -6 O-CONTROL Weight of Sample Grams 16.2 16.5 16.3 Milligrams Pb/Sample 0 .61 0.34 0.11 Milligrams Pb/100 gm. 3.76 2.0 6 0.68 X * 00028?1 . Reported: 11-8-60 Material: Treatment : Submitted: Required: Samples of blood and urine collected from a dog used in the Lead Alkyl Inhalation experiments. This dog has received 15 periods of exposure (each of 7 hours duration) to the vapors of TML in air at a concentration of approximately 0.012 mg. TML per liter of air. 9-14-60 by Dr. R. K. Davis Concentration of Lead in blood and urine samples. Analys is Number 0A-6916 Description ie-9-i>6o-6u Volume ml. 48 Mg. Pb/ Sample 0 .15 6 Mg. Ph/ Liter 5.26 The sample of blood -was lost (Analysis Number 60A-6915)* (Description - 12-9-13-60-5B) ^ gr 0 0 0 2 8 2 2 Reported: 11-9-60 Material: Treatment: Submitted: Required: Samples of urine collected from rats used in the Lead Alkyl Inhalation experiments. TML-rats are being exposed to the vapors of TML in air at a concentration of 0.012 mg. TML/L. of air. Urine was collected from the glass exposure chamber following the 43rd through 64th period of exposure (each period of 7 hr. duration). CONTROL-rats are being exposed to room air with no added lead. 11-2-60 by Dr. R. K. Davis Concentration of Lead in samples of urine; Analysis Number 60A-8135 60A-8136 Description 2 9 7- H - 2-6 0 -TML 29 8 -II-2-60 -CONTROL Volume of Sample Milliliters 105 115 Milligrams Pb/Sample 0.204 0 .028 Milligrams Pb/Liter 1.95 0.24 H ^ 0002823 Material: Treatment: Submitted: Required: Samples of feces collected from rats used in the Lead Alkyl Inhalation experiments; ' TEL-rats received 139 periods of exposure (each of 7 hr. duration) to the vapors of TEL in air at a concentration of 0.012 mg. TEL/L. of air. TML-rats have received 38 periods of exposure (each of 7 hr. duration) to the vapors of TML in air at a * concentration of 0.012 mg. TML/L. of air. CONTROL- rats have received 139 periods of exposure (each of 7 hr. duration) to room air plus dry nitrogen with no added lead. 10-3-60 by Dr. R. K. Davis Concentration of Lead in samples of feces. Analysis Nimber 60A-7440 60A-7^1 60A-7442 Description 2 78 -9 -3O-6O-TEL 2 79 -9 -30 -60 -TML 280-9-50-60-C0NTR0L Grams 24.6 20.5 23.0 Pb/Sample 0.98 ' 0.35 0.21 Pb/100 1 3.98 1.71 0.91 00.0282-4 Material: Treatment : Submitted: Required: Samples of urine collected from rats used in the Lead Alkyl Inhalation experiments. TEL-rats are being exposed to the vapors of TEL in air at a concentration of 0.012 mg. TEL/L. of air. Urine was collected from the glass exposure chamber following the 144th through 150th period of exposure (each period of 7 hr. duration). CONTROL-rats are being exposed to 52 L./min. of room air plus 0.1 L./Min. of dry Nitrogen - no added lead; 11-2-60 by Dr. R. K. Davis Concentration of Lead in samples of urine. Analysis Number 60A-8 13 7 6 OA-8 13 8 Description 29 9 -II-2 -6 O-TEL 30 O-II-2-60 -CONTROL Volume of Sample Milliliters 29 48 Milligrams Pb/Sample 0 .10 8 0.007 Milligrams Pb/Liter 3 .70 0.15 , 0002825 Reported: II-9-60