Document omz7ZjdXoRYEpdxQ6prvvnL9D

EMPLOYEE RELATIONS DEPARTMENT MEDICAL D1715ION HASKELL LABORATORY FOR TOXICOtOGY AND INDUSTRIAL MEDICINE DATE June 24, 1971 RK-1 A, ZAP?, JR,, DIRECTOR Jit. Ve l l l a b o r a t o r y ' ?rOYEE RELATIONS DEPARTMENT MEDICAL RESEARCH PROJECT MR-10-A-11 PART I :.T ' l.WESTIGATION OF THE INHIBITION OF $-AMINOLEVULINIC ACID DEHYDPASE BY LEAD .v:ority is requested to undertake the investigation identified above and T as follows: ` Irement of the enzyme ALA dehydrate has been advocated as a means of y workers exposed to lead. It is believed by some investigators that : _ vi i : tion of the enzyme ALA dehydrase is a test-tube phenomenon and not an t >. . .;o estimation of what is going on in the body. It is thought that when . : cells containing the enzyme are lysed (broken) in the test tube that is on the outside of the cell comes in contact with the enzyme and : )tii is the inhibition that is measured. In ler to test this hypothesis, blood from dogs with normal ALA dehydrase .v It- - '.11 be diluted with blood from dogs with severely depressed ALA dehydrase If the inhibition is a true in vivo (body) effect the results obtained. / J be .. straight line. On the other hand, if it is merely a test-tube effect .. will be curvilinear. 1 : st involved covers feeding dogs lead to depress their enzyme activity, VLj..deal analyses and analyses of the data. The oral section, biochemistry ; '\vi:... sgy section will cooperate jointly in trying to solve this basic funda- a net. tion. i 't c . :sted $5,000.________ ' 1 v_.- 1; authorized___________________ . - data ___________________ to be charged_______ ___________ Work to be started Immediately Request for study made by Mary E. Maxfield _______________ If request meets with your approval, please enter the account to bo yr.i have two copies authorized and return them to Haskell Laboratory, _' L dec.. i.on. The additional copies may be retained for your information iistvi:ion. ORIGINAL SIGNED BY ALEX AZAR, M. D. Proposed by__ Haskell Laboratory N25876 Authorized ORIGINAL SIGNED BY JOHN A. ZAPP, JR