Document omr2p6n029aVd86ZV6092E0Y3
AR 226 _ 3272
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TRADE SECRET
Study Tike
H-25914: Static, Acute, 48-Hour `Toxicity Screening Test with Daphnia magna
AUTHORS: Laurie D. Bouchelle Robert A. Hoke, Ph.D.
STUDY COMPLETED ON: August 1, 2003
PERFORMING LABORATORY: E.I du Pont de Nemours and Company
Haskell Laboratory for Health and Environmental Sciences
~
Elkton Road, P.O. Box 50
Newark, Delaware 19714-0050
LABORATORY PROJECT ID: DuPont-13349
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CERTIFICATION Wfreo,mtthheisunstduedrys.igned, declare that this report provides an accurate evaluation ofdata obtained
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STUDY INFORMATION
Synonyms/Codes; H-25914 1
Ssbmiters Notebook Nome RNNNINY.
Haskell Number: 25914
CAS Registry oerS|
Compaen _
ric: JN)
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Physical Characteristics: Amberliquid
Stability:
The test substance appeared to be stable under the conditions of the study; no evidence of instability was observed.
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Sponsor: UWEiLSlmAdiungPtoonnt,dDeeNlaewmaoruers19a8n9d8Company
Study Initiated/Completed: July 22, 2003 / (see report coverpage)
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Company Sanitized. Doss not contain TSCA CB:
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SUMMARY
dTehteeramciunteedtionxiacnityuonafeHra-t2e5d9,1448-thoouthre,
water static
fla, test.
Daphnia
magna
(less
than
24
hours
old)
was
cmToehneacsnetnutterdmayptweiaornastwuciroteohndf1u0c2tt0ee.sd4twoiCrtg(harn4anicgsoemnsocfeinn2t0er.aa3ct-hi2o0cn.hs5aomCfb).eHr-.2O5nB9ea1s4teesdatnocdnhaavmidsibuleaurtliwoobansswearutvsaeetrdcpoenrtrtoelstat a
water control 20 precipitate
and the 0.1, at est start.
1.0, Al
w1a0taenrdqu1a0l0itmygp/aLratmeesttecrosncweenrterawtiitohnisnwaecrceepctlaebalrealniidomnicsto,slodtruhlereisdnsiglwutithtiehon
exposure.
4E18.x0p,hoo1su0ur,sr.aenodNfow1a0it0memmrogbf/ilLelairtteyosuotlrhteesuddbiillneutt0hi,ao0ln,ew0fa,ftee1cr0t,scaownnetdrreo1ls0ae%enndiminmnoomtbihienliadtliylH,ut-ri2eo5snp9we1ca4ttiecvreolncyco,enntatrtortlahtetieoesnnsdofof0.10, Torhgeanlioswmess.t nTohmeinhailghceosntcnenotmriantailoncocnacuesnitnrgat1i0on0%caiumsmionbginloitiymamtobtielsietnydatwatesst>en1d00wmags/L1.t.0 mg/L. Nominal H-25914 concentrations were used for calculationofECso values The resultsare summarized as follows: -- N48o-mhionuarlEcConscefnotrrHa-ti2o5n9s14of,Hb-a2s5e9d1o4n,. mg/L | dilution water control, 0.10, 1.0, 10, and 100 nominal concentrations, mg
H-25914
using the
ewxahtiebritfleeda,lDoawpchonnicaemnafgonraaq(ulaetsiscthhaanza2r4dhouinrsanoludn).aerated,
48-hour,
static,
acute
test
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CompanySanitized. Doesnot contain TSCA CBI
SSHc-r2e5e e9n1i4n:g TSete satticw,itAhcuODtaepp,hi4ne8i-anHmoeuarpgnTeoanieity --
MATERIALS AND METHODS
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A. Test Solution Preparation OTTFehseHtsLstuWobcWsktasinolcnueatsi1oolnLu(tg1ilo0ans0ssmbwgee/arLke)eprwraaenspadprrsetediprbarryiendgdiflbouyrtiaaopdnpdrifonrxgoimampaaptsretoloxycik3m5astomeliulntyuito1en0so.0fmHTge-s2ot5fs9Ho1-l4u2t5ii9n1H4LtWo W1 .1, aprnedpsatrierdribngyfaodrdaipnpgrtohxeimaapptreolpyri1a0tmeivnuotleusm.e TofetshtesosltuotcikonssolwuetrieonclteoaHr aLnWd Wcolionrl1esLsgwliatshsonnbseoawkeerres precipitate at test tart.
B. Dilution Water aaDsinldCuasteiCaoOlnsewdabttyoetrbheoedrrfiolgcoikwn.-atperTdohpeforrhotamirodtnnheeedsHsaaodsdfkitetlhileonLHoafbLoCWraaWtColri.ys awTdehjluelswtHehLdictWohWaisppi3srt7o8hx-eifnemaeaettredaletyeedp10a0n-d14is0cmags/eLd. rtehrmoouvgehpaargtrieceunlasteasn.d Tfihleterwtaoterremisohveeatireodn,oranchdilflieltdearesdatphprroouprgihaSt0e-,an1d0-d,isatnrdib3utemd t,fhirplotaeusrgsshetdoaged ~~ pcaotliyovnisn,ymlecthallor,itdoetaplipoirngga.noTchhleodriilnuetiaonndwcartgearniospahnoaslpyhzaetdeptewsitcieciydeeasr,lyafnodr pmoaljyocrhlaonriionnasteadnd
biphenyls. The dilution water meets OECD" and ASTM specifications.
C. Test Organism Culture fbDiealaptkehernerdisaH(1mL0aWgpenWrabhweeealkrdeeartraet1a9rc.eu8dltCau.treHDaiasnkpitehilnaltiidLoasn)bwowerhraietcofhreycdoionnnt2aa5i0ndea-idmlL2y0b(01amspiLesrwobiretah1k2e0r0m)0LomrLL1oo0f0faa0e-rymeaatLsetdP,,yrex tcheeregarleleenavaelgsa,anSdelteronuatstcrhuomwc(apYrCiTc)ormniuxttuumr,ea(tsatarnadtaeordfiz6e2d,t5o0017c0e0lltso/m2L1o0f0cumlgt/uLretomtealdisoulmi,ds)Tahned 2Nc8oe-modbnaiayntaeotsliduospnaerodefinYntCtdThaipshantneisddtsa.wlegraSeiicslkeensqesustishv,aanil2nejn4utryht,ooau0rn.sd1-oa0lb.dn2oamrnmedaltwioettraileesocrowglealrneeicctneocdtarfsbreooenmn ptaehnredd1ea2ppthhhniipbdpr.ioaodweorfe c{nuoolrtttubereseitnibngegapkdreuorrdsiunacgneddthtebeys4t8t-hcehhoapumarbreeprnrste.tdeaspthpneirdiso.d.NDoapadhunlitaimmmaogbnilaiwteyrweasidseneteinfiiendtbhye claubletlusreosnutsheed
D. Test Methods --_ cFoonucrenntormaitniaolnscownecreent0r.a1t0,io1n.s0,an1d0,aadinldut1i0o0nmwga/tLerHc-o2n5t9r1o4l,were used in this study. The nominal
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sdDoualrpuihtningointashemwataegsstn.caoAmnpdeldoeinttaeitdoe.nso,Ifmlmedsaasbpithhlnaiintdys2a4tnohdoteusbrteshsoaolvldiu,otirwoaenlrseowbusaseservdianiitntiiotanhtsieswd esatfrutedeyrm.amidDxeaipnhdganioilyfdtsewetreestnot fed
ererion for the effect (immobility) was a lack ofreaction to application ofa gentle.stTihmuelus.
tA1h6erhe4oc8ui-rrhscoulluiargthittensg(tawapatptareporpxbriaomtxahitmewalaytseSlu7ys5e2td0o.t47o3mC8aiwLniuttxah)iananamdnega8enhootuferm2sp0.ed3raart1k0unr2ee0s.si5nwtaCh.s etAemsptplhcoohtyaoempdbeewrrihsoidcduorfing
liingchltuidnetder3va0l.minutesoftransitional
light
(19
10
52
Lux)
preceding
and
following
the
h 16-hour
ddDaiilpsuhstnoiilodnvsewdwateoerxryegcaeodnndtrecoodlnacatenntdterttaetssittoarnst,u,bpasHnt,adnaactnedtceotsnecmeepnnetdrraoattriuaortnest.wotearlTehiemmsmeeoabsmiuelraiestduyriiennmaaellnrterpeslpilwcieacrtaeetectsohaakfmetbnhebeefore tTeessttesnodl.utions were not aerated during the test and were disposed of in an appropriate manner,r at
--_ E. Results and Conclusion
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horeganlioswmess.t nTohmeinhailghceosntcnenotmriantailoncocnacuesnitnrgat1i0on0%caiumsmionbginloitiyamtmotbeisltietnydatwatesst>en1d00wmags/L1..0 mg/L.
The 48-h uHs-i2n5g9t1h4e
our ECs, for H-25914,
exhibited low concern
based on nominal concentrations, was greater
for aquatic hazard in an unaerated, 48-hour,
water flea, Daphnia magna (less than 24 hours old).
than
stati
1
c,
00 mg/L.
acute test
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REFERENCES 1. OTregsatniinsgoatfioCnhefmoircEaclso:no2m0i3c,C1o7-OJupleyra1t9i92o.n and Development (OECD). Guideline for the
2. `ACmoenrdiucctainngSoAcciuettyefToorxiTceisttyinTgesatnsdwMiattherFiiaslhses(,AMSaTcMro)i.nve(r1t9e88b)r.ateSst,anadnadrdAmGpuhiidbeiafnosr. E 729-882. Annual Bookof ASTM Standards, Vol. 11.04.
3. uSnmdrecrheTkS,CJA,:Clmeemtehnotdss, aR.n,dMeovraclauactki,onRo.,fadantda.RabEenrvti,roW.nme(1n9t9a3l).ToAxsisceoslsoignyganecdoRliogsikcal hazard AAsmseersiscmaenntS,ocAiSetTyMfSoTr PTes1t1i7n9g.a(ndW.MGa.teLrainadlis,s,Ph1.i5l.adHeulgphhieas,, and M.A. Lewis, Eds.) pp 22-39.
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TABLE 1
DISSOLVED
OXYGEN
AND pH OF TEST AND DILUTION SOLUTION SAMPLES
WATER
CONTROL
Nominal H-25914 Concentration (mg
Test Initiation (0 hours
Dissolved
Oxygen
mg
pH
Test Completion (48 hours
Dissolved
Oxygen
2
3
Dilution Water Control 86
0.10
85
73 73
87 87
79 79
10 10
85 86
73
87
75
87
79 79
100
86
71
85
79
TABLE 2
rr
IMMOBILITY OF Dap4h8n-iaHOmUaRgnTaEISNTAWNITUHNAHE-2R5A9T1E4D, STATIC, ACUTE,
`Nominal H25914 Concentration
g
NNuummbbereratISmtmuodybilStea/rt [io [| u shows s |
Dilution Water Control 0.10
0/10 10
010 0/10
10
010
10
Loo
10 1/10
.
100
010
1/10
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