Document omOxn9a0D4kjE32Q3dbD8xDRR

AR R26 - 0716 ACUTE TOXICITY TO FISH (BLUEGILL SUNFISH) IESTSUBS ~~ T~AN 00 CE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonic acid) (CAS # 2795-393). Remarks: is FC-203. The 3M production lot number was not noted. Current information indicates itis a mixture of The test sample 1.34% PFOS, 36% diethylene glycol butyl ether, 37.85% water, 20% ethylene glycol, 2.66 % Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyliriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD: 0000000 Method: Fish bioassay procedures, Standard Methods, 1970 edition. Type: Flow-Through Acute GLP: No Year completed: 1972 Species: Lepomis macrochirus Supplier: Commercial hatchery in Nebraska. Analytical monitoring: Temperature, pH, conductivity, and DO Exposure period: 24, 96, and 264 hours. Statistical methods: TLs (incipient median tolerance limit) values calculated using a linear regression equation. Test fish age: Juveniles Length and weight: Average length = 56 mm Loading: Not given Average weight = 2.4 g (wet) Pretreatment: None - Test conditions: Dilution water: Aerated well water Dilution water chemistry: Hardness: 38 mg/L as CaCO, Lighting: Not given. Stock and test solution preparation: Sufficient amounts of stock solution containing the compound initially added to each test vessel. Proportional diluter used to maintain desired concentrations. Flow-through rate: Continuous at 5 L every hour. Stability of the test chemical solutions: Not noted Exposure vessels: 30 L Numofrbepliecatr es: 1 000519 Number of fish per replicate: 30 WNautmebrecrhoefmicsotnrcyendturraitnigontsh:e seven study: plus a blank control PH range (0-96 hours): 7.1-7.1 (control exposure) 6.86.9 (33.4 mg/L exposure) Temperature range (0-96 Dissolved oxygen range hours): 18 + (0-96 hours): 1C 9.1-6.3 mg/L R0 ESULTS 0000 Nominal concentrations: 33.4 mg/L Blank control, 5.9, 7.9, 10.5, 14.1, 18.8, 25.0, and Element values: 96-hour TL50 = 20.4 (16.4 ~ 25.3) mg/L 226644--hhoouurr TNLO5E0C==155..99 (m1g2/.L5 -- 20.2) mgiL Element values based on nominal concentrations SRuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTduhcetveadluoenstrheepmoirxtteudraepapslydetosctrhiatbemdixitn utrhee aTnedstnot the fluorochemical proportion alone. co0 nclusion0 s 000 The FC-203 mg/L with a 86-hour TLso for 95% confidence fathead minnow was determined interval of 16.4 to 25.3 mglL. to be 20.4 Submitter: 3M Company, Paul, Minnesota, 55133 Environmental Laboratory, P.O. Box 33331, St. p0 atA0 QuA0 LY 000000000 Rperloiacbeidliutrye:s Knioitmciosncshirsatnekntinwgit=h3.curTreesnttirneggwulaastodroynaeppursoiancghleasb.oraTthoerysample ~ pcuorniftiyrwmaatsionnootfptrhoepearmlyoucnhatroafctfelruiozroecdhearnmdictahlepsrtoupdoyrtliaocnksinatnhaelystoilcuatlion. RE0 FERENC0 ES 0000 Test was conducted by Bionomics, Inc., the 3M Company, St. Paul, MN, 1672. of Wareham, MA at the request of o0 wer 00000 Last changed: 6/27/00 000511 J ARB E i tara dGa e C nrdsa % Co I CS f RE EET a N E gee 4o: eao Gi an SECM He hn SE pa bE HAR: HE Ia 1 10d 5 3 pa SER t R GE 4 y 3 R Rr O P eiiTO CEE % i io A A Hii fry ihLoa 5 Lak Sars i uo Hot ai ERIS SAA Ea . 3 i g. LT 3o : afegr i Be Lie 30 gE RH 4:4 ULE . CH AL ETE onR C e C a he iv E bhyi HolLit ihda e een pan ! ) Gi o IS aER T BEST COPY AVAILABLE DAY ERe?eW nERe Sea as 2 000512 METHODS AND MATERIALS The investigation was performed at the aquatic toxicology laboratory of Bionomics, Inc., Wareham, Massachusetts. The susceptibility of bluegill (Lepomis macrochirus) to Light vate aqueous i1n forming foam (tested as 100% active) under dynamic conditions was reported as the incipient median tolerance 1init (TLgy), the concentration of the test compound in water causing 50 percent mortality with no additional significant response (>10%) during the final 48 hours of exposure. The predicted TLg, value and its 95% confidence intervals were arrived at by converting the concentrations tested and the corresponding observed percent mortalities to logs and probits, respectively. These values were then used to calculate a linear regression equation. Test procedures for the dynamic bioassay are those described for fish Bioassay Procedures in the 1970 edition of Standard Methods (APA). The bluegill were obtained } from a commercial fish hatchery in Nebraska and had a mean weight of 2.4 g and a mean length of 56 mm. The dynamic . bioassay was conducted using a continuous-flow proportional dilution apparatus (Mount and Brungs, 1967)%. Mount, D. I. and W. A. Brungs. 1967. A simplified M dosing apparatus for fish toxicology studies. Water Research. 1.21. 000513 NE ilies ana. aeSB ARE t fas s ag i Si oi Page two . The apparatus provides for intermittent introduction of seven concentrations of the test compound into test vessels and diluent water to a vessel serving as a control unit. Flow rate to each of the 30-liter test vessels was 5 1/hour throughout the test period. The test diluent consisted of aerated well water of pH 7.1, total hardness 38 mg/l as CaC0,, and a constant temperature of 18 (* 1.0). Dissolved oxygen levels for the test ranged from 9.1 to 9.3 mg/l. Thirty specimens were introduced 48 hours prior to the start of the assay into each test unit. The desired concentrations of the test compound were established after the 48 hour acclimation period in the test vessels by adding sufficient amounts of stock solution containing the compound to each test vessel. The proportional dilution apparatus was then used to maintain the desired concentration of the compound in each test vessel. RESULTS 3 - The predicted TL, values and 95% confidence intervals : are presented in Table 1. The data for p,p' - DDT, determined : at Bionomics by a static bioassay to serve as a "standard" indicate that the population of test animals iS representative of an "average population". Table 2 presents a summary : of observed mortality for bluegill after 24 and 96 hours os dtm ltt 000514 Bm aed Lt EE rrtS fee nigh o Page three of exposure and end of test. Moribund fish generally became dark and lethargic, lost equilibrium, and expired. Table 3 presents the pH of selected concentrations from the test at 24 and 96 hours andend.of test. FMoarm tgbhee : : 000515 sn aiAl abeeret . Ag bce op ARRa Bl Se 1 sot SUBMITTED BY: Bionomics, Ine. 790 Main Street Wareham, Massachusetts September, 1972 PREPARED BY: ' Bevier Hasbrouck Sleight, III Chief, Acute Toxicity and Residue Investigations APPROVED BY: ; Kenneth J. Macek, Ph.D. - Director 4 . ) " 000516, - Sion cesor YOame raliebul ica ae se a cA PR emi YE EIS eR ligt SRP  Table 1 -- Acute toxicity of Light water to bluegill? (Lepomis macrochirus). The data are based on dynamic bioassays conducted at the Fish Toxicology Laboratory of Bionomics, Inc. in Wareham, Messachusetts. ci :7 ; No Ef Compound Th E ereto (ng/1 -- re -------- etme--eet rent Se p ----e --es Lignt ated 533.4 20.4(16.4-25.3) 15.9(12.5-20.2) 5. DDT _-- 0.008(0.004-0.012) "assay conducted at 18 (* 1.0) mean weight of bluegill 2.4 eg PIncipient Lg, estimated over 264 hours. 95% cofidence intervals. wR 4 1 :_ C . Ta . i c Te Ss N dli ni ib 1e 00i0sh5t 17mit [ENR eb i en AA isl ts ' Table 2 -- Concentrations tested and corresponding observed r percent mortalities for bluegill (Lepomis macrochirus) e of teste . e exposed to Light wate after 24 and 96 hours and end ------ Concentration (mg/1) 33.4 25.0 18.8 wa C ao0.s 7.9 5.9 Control our 0 0 o 0 0 0 .0 ee z mortality observed our Incipient _ 264 hours 100 100 30 Im 17 50 10 2 o 17 0 3 o 0 0 0 eh i BEST COPY AVAILABLE .: Si fea . Chien a ne IE Ldsaly di St aki ns a RE at NS E A e ET 5 000518 8  -_-- Table 3 -- pi of selected concentrations from a dynamic bioassay conducted with bluegill (Lepomis macrochirus) exposed to sient wate aster 24 and 9 hours and end of test. - &Goaggyrration 2% hour oH. 3 hour Tncipient CSacninonsb SiAeReEeEeSLiRnhLeersi 3b EE che re ET ele THe Lom Wy ETE PALER ly FerAl 59 3 7.4 id 7 Ea ee . comtrol | 7.1 SE a ETAT fr : g chy .A . 2 Te, ol > 7 aT Co dlsbe kee Be JalSiEE AR E eh CR hadSE 5E eo. 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