Document omLJv2Qq1OaXO2M7jNdjRoqE7

hoo. 3 lh faster - Sepsanber 20, 1976 1 / | CCO ON NF EI ID DE ENNTTIIAALL 1 nterottice Correspondence SO 5 o-- m -- Stste amaigrinod segues SEL 3 SUbject: ,cccci:llil..EE.. FFrreeiieerr -= 220011--I1S8 Analytical Request A62781 Ei] 3ijJBU..i.o5DEkF).... oNbLlilioaanapnZgggggeeeorrnttl-eed-t22--2230-00-12222-3328E16600W-111-15 September 20, 1976 3 B.W. Nippoldt - 2a01-iIS m 4 ~derar~ss__n- 290-2E --F5PF.6A.a. WUrebieablle-nn2a-202-l20~1-15 8 0: D.G. 3.WeiLbOlNeGn-- TO2K0I1C-iOSLOGY - 220-28 ` TFORO:M: J. J. LBOENLGISL- DTO-XAINCOALLOYGTYICA- L 2R20E-S2EEARCH = 201-15 . FROM: J. BELISLE - ANALYTICAL RESEARCH - 201-1S AASnnoaamllpyyisostiiessa"ooff tTthhwee ssseeerrruuunmm ffsrraomompmlemmsiicceewarffeeeddsFFuGCbm--i88t00t77edaa:tt IBT IBT has has been been | completed. Two serum samples were submitted: @((2i))) cCEooxnnpttorrsouorlle - obtained by combining the serum from 2 groups (2) GE1x"poossutremi-ceo,bta`oinen.edfodb'y10c0o0mbipnpmingRC-t8h0e7seanrdumthferoomth2ergroups . oaaTftteprt35ee05ss00e00tntmppsopinmct..e'h,e hoAfsneeeeadaifnefFgdiirross1ftt000a2a0pp0ppp0prrmooxxpiiFommCmaa-tt8Fii0Coo7-nn8;,0a7n.dtthhiitsshesseoertruhuemmr : represen~ the feeding of 2000 ppm FC-807. o-. HSsuaanmmapplnlee- Literature AverageOorrggaannii0cc.03FFlluupooprrmiinnee Human - Literature Average 0.03 ppm CCoonnttrrooll MMiiccee ~? eesstt.. 00((00--00..55))11 ~Exxppoosseedd2? MMiiccee 110022 . eesstt.. Vtree~~y, FFlluucooorrii2ddeeem 0.02 ppm 00..55(000..55--11..55))11 0o..66 mina? <ouov BE, oot seporsas Animals3 < 0.006 1 ~ 1 insufficient sample for accurate analysis fe elis? ,e 2, not reported i insufficient sample for accurate analysis 22 approximately 2000 ppm FC-807/30 days andoli' +0 3 3 animals GGuuyy aanndd animals (Fats TTaavveess (rats nor studied) as rreeppoorrtt oorrggaanniicc not studied) as legs than 0-006 ppm. fflluuoorriinnee lleevveell iinn're~lieevveenn less than 0.006 ppm. ddiiffffeerreenntt i { hTTThhhhdeeeeiceFefaxxlltttuuerroosaarrccoottcctiihhhooeeannmntiiccttaeae`ocllcohhhnwnwiaaioqssqfuuee.iitshseoo((llIIIaafCCttLl1eeuddaoanrndodffcrrhegoo~acmmthnhaittercrhh))aeol ssffweeooarrllsuullnmoowwiooeenffdd tbbhttyyehhee eaaetnenxhaxaplelpyoryossssiieeessddxtmrmiiaccceet.. ; { | S i((ni4dieacxratttrreaaE sccttiitoohnnasst)) wW8'I0I6%IniTleoetfh^er.st2twh0ae%tefrsrleoummpooahirianonseceedhd:emIitirnc~athe~l awaotsrGeumeiarxntpaeettrhdiemeeDntrthose~.relanerxepthraascet: and less t~an-'5% in the water phase. ~'ur~-er experiments are nneecceessssaarryy ttooe-essttaabbllilsshmIifz tthhee f~lluuoor~oocchheemmiiccaall iiss ssttiillll pprrootteeiinn Bound. if a different fluorochemical is bound, of simply not extracted boun~ if a different fluorochemical is bound, or simply not extracted Be the ether: . . fon HATE ;|3% -~ 7C+ udd.le 4} : <S .:./.,A J,-I ! Gl os Mer IPL1E i Aarliiyn ] Exhibit 1133 4 Sibiect a Frotecive Order In Famerv. 3W, Na 67.04-6305 MMaaddee AAvvaaiillaabbllee bbyy 33MMffoorr IInnssppeeccttiioonn aanndd CCooppyyiinngg aass CCoonnfifiddeenntitiaall IInnffoorrmmaattiioonn:: Subject to Protective Order In Palmer v. 3M, No. C2-04-6309 Come vom 33MMAAQ000096677440066, 11113333..00000011 i. J f T -2- + ~HMMRR ooff tthhee eetthheerreexx it (and this represent about 75% of tsao s ether f psszaat (and thls representshoutTt of, ~ne ~luoroc.emical present in the serum solids) establishes 1. EER feria } the fluorochemical to be most likely CSFI7SO3H. 4s a follow-up experiment, 1 ng. of FC-07 as added to 15 mi. As a follow-up experiment, 1 mg. of FC-807 was added to 15 ml. fog Ae SI A RE ; of Red Cross plasma and the sample wor~md-up using the above oF Sad CoonsJe a Sip takrlsvoieh.yy Soin, thysure procedure. NMR of this ether extract indicated the fluoro- chemical to be FC-807. This is good evidence that the b.gdlyy ScEhaSmicSalttheobBeat5o-r00T7I,T BotShGiAa ls Sgoeodtaevidetneceor TELMchat bonwdedy Fo the serw and not the chemic~ woyk-~up is responsible for the s,ulfonic at~d a~ the ma~or (if.,no~i only)~m~tabolite of FC-807 bound to the serum. concussion CONCLUSION CA Mice fed FC-807 at a level of 2000 ppm (see Jsaamgptlee description) fige Sod FOS07 95 Sues,of 200 rm, (enesuupiysegery k for a period of 30 days develop a fluorochemical level of Erinitot 8feydylop v tlle] Itor ; CgFL7SOI approximately I0,000 greater than the control. T~e fluoro- cchheemmiiccaall iinn tthhee bblloooodd iis a metabo~lite of FC-807, namely. TATA WHS ; Neutron Activation Analysis 1 spoke vith Uz. Touts of Generel hekivevion Mualysis Feparding I spoke with Dr. Kovar of General Activation Analysis regarding L,2pgke, vith og. KoranofConoralActivaAtnailoysnis regarding the analysis of total fluorine An bloo~. H6~ndica~ed that they BE To 7 Saf ore win oo k had never run this determination but felt it would be possible Fa TL using a linear accelerator. The cost.would be $300 for the _ ddfiiiissrrccssuuttsssssamttphhleeessuaubnbdjjeecc$tt80 iinnpermmmorraeeddiddteeittoaaniialllwwiisttahmhplGGeAA.AA aan~Iddtshshihnookuullwddeccoosnnhssoiiuddleedrr 1 wm Same e wiblectuhSuse Sohal IRR an sheuidcenter sending them several samples as a check on our analytical results. $e 5180 52 . ]] 1 | Nd a A CPTR CoB Made Available by 3M for Inspection and Copying as Confidential Information: Subject to Protective Order In Palmer v. 3M, No. C2-04-6309 11113333..00000022 sassrior 3MA00967407