Document oevG11526K6qXKyB9kMEkVxGw

AR 826. 06T4 TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity: A mixture containing perfiuorooctanesulfonate, which may also be OrecftearnreesdutlofoansicPFacOiSd,) (FCCA-S95#o2r7a95s-a39c-o3)m.ponent of FC-201AF. (1- Remarks: _ The 3M production lot number was not noted. The test sample i1s8F.C5-92%0d1iAeFt.hylCeunreregnltycionlfoburtmyaltieotnheirn,di3c7a.t8es1%it wisataerm,ix1t4ur%eeotfh3yl%enPeFgOlSyc,ol, 38.%2t5r%ieAtchraynloiclafmoianmee,r6, %9%sulslofdoinuatmedocatlyklylsueltfahteer,s0al.t3s,%asnoddi0u.m05la%urtyollyslutlrfiaatzeol,e. cThhaerafcotlelroiwziendg scounmcmeantrryataipopnlsieosftiompaurmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect the toxicity of the fluorochemical component of the test sample. METHOD: Method: OECD Guideline 201 and EPA CFR 40 797.1050 Type: Acute Static GLP: Year stNuody performed: 1995 SSpoeucricees:: ISne-lhoeunsaestcruultmurceasp.riOcriogmiunatlulmy from ATCC, Rockville, MD Element basis: Algal cell counts (cells/ml), specific growth rates. Exposure period: 96 hours Statistical Analytical mMoentihtoodrsi:ng:NotNoatppgliivceanble due to study results. Test Conditions: Algal Nutrient Medium: Nutrient medium for freshwater algae per OECD method. This medium served as the diluent for all algal operations. The pH of this Stock saynndttheetsitcsaollguatlimoendpiruempawraasti7o.n9.: A 1000 mg/L primary stock solution was prepared by diluting 500 mg of test substance in 500 mL of standard algal netrient medium. Aliquots were then added directly to dilution water in tEexspt ovseussreelsvetsoscerlesa:teNtoetstgisvoelnu.tions. Agitation: Not given. Numberof replicates: Not given. INnuitmiablecrelolflcooadnicnegn:tra1txio1n0s:ceOlnles/,mLplus a blank control Lighting: Continuous Water Chemistry: Not given. 000191 RESULTS Nominal concentrations: Blank control, 1000 mg/L Algal Growth Response ECs; values Ex(Cpoonstaucrte) | MeSpaenciPfeirccGenrtowCthhanRgateein EC50 (mg/L) Hours (1000 mg/L Conc.) [2a 7 "13 7 >i00 | IN ES >1000 eo | 18 | >t000 | Element values are based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical components alone. CONCLUSIONS The test substance exhibits a 96-hour ECso for specific growth rate of >1000 moll. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 pAtAQuALTY Reliability: Kiimisch ranking = 4. Not a laboratory data could be located. Tmheearseurisemneoncthaorfacttheertiezsatticoonncoefntthraettieosntss,uabnsdtacnocnefiprumraittyi,onaonaflytthiecaalmount of fluorochemical components in the solution. REFERENCES ~~~ LThaibs Rteesqtuwesats ncuomndbuecrteNd32b8y1,th1e9395M. Environmental Laboratory, St. Paul, MN, THER Last changed: 6/26/00 000192 06/25/2000 ENVIRONMENTAL LABORATORY 1 ARCHIVED FINAL REPORT LAB REQUEST NO. N3281 REQUESTOR NAME: SBS/SHARI LOUSHIN DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 09/28/1995 DESC: FC-201AF LIGHT WATER CONTRACT LAB(S): EXP COMP DATE: 12/04/1995 DATE COMPLETED: 01/17/1996 PROJECT LEAD: TL HATFIELD PHONE NO: 651-778-7863 3M FAX NO: 651-778-6176 Results for toxicity to fish, Daphnia, Microtox and activated sludge resp. can be found on lab request N2964 sab 10/3/95) Standard testing for AFFF products. Formulation is on Doc. # 07-2573-9 in PRISM. 3M ID number given as ZF-0002-0843-7. sab 10/3/95 Results from algae toxicity test: Test Conc. ng/L 1000 Mean Percent change in Specific Growth Rate 24-hrs 48-hrs. 72-hrs 96-hrs 13 14 22 18 EC50 >1000 >1000 >1000 >1000 sab 1/16/96 SAMPLE DATE CODE DESCRIPTION 1 09/04/95 FC-201AF Dark Liquid IC50-ALGAE ALGAE ASSAY - IC50 RESULT >1000 MG/L MOL or (95% C.I.) -) 000193 BEST COPY AVAILABLE ENVIRONMENTALLABORATORY : FRESHPWRAETPEARRAALTGIAOGNWROORWKTSHHEIENHTIBFIOTIRON TESTTM LRSEE BELOW PacEe os r DATEIOA AS. PT _-- a eal ick sale pepaainadlUioSnS, Be Talal Tome eae ee ii5y m 2 YZn oPdApePd EnSec3eEs CT SoTntRYRadla.FyrJedPURdI ciui ptivweeeeHone kuaS f e tahS eno| ot BBroa roinaAo un omnecrelsle]airl WgreSCIDahu[orY raeraan scal. [1282045 OLEe CD2r01 ais, cGiroocnhsiionnihooonnTionsntgandsFatABVils4.5 877571sem0is 50MgtA(ToEmS-- eEAT R-- H ER roanasa SSAEER E JO 22SR N n aESRERIeR n TAe 8 1 ereoTl, i Gant aE ra rn or y ae a a 0001. : BEST COPY AVAILABLE STANDARDENNUVTIRRIEONNTMMEENDTIAULMLFAOBROFRARTEORSYHWAATLEGARE NUTRIENT INcSoTnOcCeKNTSROALTUITOINON FIINNATLECSOTNSCOELNUTTRIAOTNION STOCK SOLUTION 1:MACRONUTRIENTS "wnNaaattMeo1sd655 xazs1 NMcaaHCclChy 264H0O 1z158 opott KHMgSOO,," THO o15t6ooLt STOCK SOLUTION 2: Fe0TA FNeacglyDT6A0240 1a00 mmoo.r STOCK SOLUTION : TRACE ELEMENTS 1Mn8C0l, 40 20Cos, 640 cNahda2, 020 41855 mmoort 1a5 mmoolt 7001 mmott 111258 mmmoooltt. 1156mmoott 1000 uutt a155 wwott 315 uwtn 70010w0t1 3 STOCK SOLUTION 4: NaHCO3 : NaHCO, 50 ot 50 mot. Strastocksobons 1-4bymembrana fain (02 um). 2: Fi. olSOLUTIaONSt .NUTaRIENn T CoNo CENTi RATEIEEA srR1pi1 y ALrEaRdEsAEaSn es aa SURal LL x AT I omastoosoumont 30 mol STOCKSOLUTION 22+ 4L1 E5 AESATES Lis 2 eEra B ogoeg CBEet Japo tassTn ioookescot aummioonno sity 4sh o4C TORREE SGTE aE 30 i La SSeS SSLoRS CARES RA ooo oe ie SeEH I eh ERY Sakae ET fei Sl 3 Soumoe cAr A rmEa ve aE TL ois iEe s na a CE 1 a i CR ARERNG dG LaEi RE ER Rr ay 3 io0A00195 soma acne TA a oy afi2c, FRESPEAS CLGGARH GORVOTW WHTHAA IAHTIBET TIONETEESRTEE657]13 +H2 TT ALGAE: stgn,r strani o oran FN E3 o LL[| [=] Jes & TSEoSnSnTing enonTsSuet none] es onT ]SisL AR | BEid sxcournor ; SH ] hsesss we |55 | EisEE3 i: wname ese | aToHe| HEARETS 5 A | ee 4 wom fo J [om] fon[ET 3 3 f i om wo fun |e : 5 E-3 1000mo. po [oe [oe Po [2] 2S Bo Sato RST tmnt nas eohipR eea2A%essi m a s WEs e Aso |s oipfes s erA s |i ajWaeeSrssTwoU]RtGEepad S E ---------- saga ET pa ---- Ry |