Document oery8GO62YYRLjY0kR73OOVD8

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:[2-(N-Ethylperfluorooctanesulfonameitdhoy)lacrylatem;ay alsobe referretdoas B1228, D-1, ETFOSEA, orFX-13.(2-Propenoicacid,2[ethyl[heptadecafluorooctyl)sulfonyl]amiensot]eert,ChAySl # 423-825) Remarks:MateriiaslanambersolidS.amplepuritayndnotrecorded3.M lot number 13. METHOD Method: ProtocolReference EARIWAS 5/9/84 Test type:Staticacute GLP: No Year Completed: 1984 Analyticalmonitoring:Dissolvedoxygen concentrations. Test organism source:ActivatesdludgecollectefdromtheSt Paul/Minneapolis, MN Metro Wastewater Planton 5/10/84 Test conditions: Dilutionwater: Deionizedwater Test solutionpreparation:Placed0.30g testsubstanceina 300 mL BOD bottlewith50 mL deionizedwater.The mixturewas mixed witha magnetic stirrinbgarand box for- 30-minutesinorderto disperse sample. Aftermixingthesample was finelsyuspended inthewater. Test vessels:300 mL BOD bottles Number of concentrations:One Temperature: Not recorded TotalSuspended Solidsof sludge on day of testing:Not recorded. Sludge was used theday of collection. Exposure period: 6.65 minutes Element Basis:Respiratioinnhibitiaosn determinedby dissolvedoxygen concentration. RESULTS Nominal concentrations: Two blank(deionizedwater)controlso,ne 1000mg/L testmaterialsolution. StatisticAanlalyses:ECsovalueswas notdetermineads theoxygendepletion rateforthe controlswas judged to be equal tothatofthe deionizedwater. controls AnalyticalMethodology: Method forthe analysisofdissolvedoxygen concentrationswas not recorded. Control response: Satisfactoryd;ifferencebetween controlswas 6.9%. CONCLUSIONS The testsubstance exhibiteda no inhibitoreyffecton respiratioant a nominal test substance concentrationof 1000 mg/L after6.65 minutes ofexposure. The EC50 (respiratioinhibitioins)thereforegreaterthan 1000 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul, Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. This studywas not conducted using an establishedmethod. Testingwas alsodone wellabove the solubilitlyimitofthe testsubstance. This studylackssufficiendtocumentation and characterizatioonf the testsubstance puritya,nd analyticamleasurement oftestconcentrations. REFERENCES This study was conducted by the 3M Company, EnvironmentalLaboratory,Lab Request number B1228, 4/l/84 OTHER Last changed: 5/22/00 3M EnvLronmentaL Laboratorg Route ToxLeLt of a Substance to RetLvated SLudge Mexed LLquor (SLUDGE-TOX) ProtocoL Reference: ERR/WAS 5/09/84, Lob Request No..BI228 7- 6C!D C.D C:3 U3 0cn 3- C3 Legend. 0 --DEIONIZED WFVTE:R'CONTROLS- RVERM 'i-13 LOT-13 (1000 "GL/L) IC50 >1000 MG/L o-+ 0 1 2 3 4 5 TIME MINUTES sq STOX.DRT 3M Environmental Laboratory Acute Toxicity of a Compound (Substance)to-ActivatedSludge Nl"d'Ltquor Protocol Reference.-EAR/WAS 6-09-'84 Lob request no.: Date: Analyst:' The activated sludge mixed liquor Wes c6llected.from the St. Paul/Minneapolis Metro Wastftmter Plant on -::E@Lo Sample description:-&,/"V,@,-./) Fy-i3,L- L/ reparationof stock solution: grams of samle were diluted-to ml Ith deionlzed reagent water. Date of preparation: c IKE Okc NTIRATION OF TEST MATERIAL '21 ._,A@T I T ED SEUDGE MIXTURE min. sec. C'M on ol- c. 7,40 7./ -7,10 @7, -7.5r-A7 .I- '7. c,. 3 1S'zo 60- 76@ 'A S"/o 6D 3.90 2-r Y 75- COMMENTS.- (A 3 oc r-k 4 er4, lq-44,