Document oeqGGkmnqNRo06449M89m0p7g

Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Page 1 of 56 Appendix A: Test Sample Re-analysis [performed by 3 M ] Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Page 2 of 56 Appendix A1 : 1st Test Sample Re-analysis Internal Correspondence TO: FROM: REGARDING: John Butenhoff November 14, 2000 Fred L. DeRoos; 201-1W-29; 6-0665 Corporate Analytical Technology Center GID-4242 Determination of Fluorochemical Residuals in Products and Product Treated Webs Introduction FdasuneobtauelmryrmsfitletuisenodewroftehocrehreeaccmnoaanriclrcyiaeeslndisbtroabausytetiodMunspsiikrnooegdfMtufhlcuectsNosraaaomnmcdheaeprmmaroeuidtcnhuadolcedtrrestsrCaeidAsautdTeaedClsscw.rreeiTqbbhuesdeewssitenaNrmtehope.slueArbse-mp1ho5aitrd1tte0bfd9oe5rfeo.nRrTepaqrhneueavelcyisoutsuirAsrsel-tyn1ot51095. Results T- h6.e rOensulyltsanfarolymtetshtehaant awlyesreesd,eatnedcteadcoarmepraerpiosortnedto. the original analyses, are summarized in Tables 1 Table 1. Concentrations of N-Methyl FOSE (ppm) Sample Identification N- (oertigin1al! E N- (ceutrre1nt! E Difference 4101W Product 4101 Treated Web 0 0 735 + 15t+ 2 %) 1.270 + 530(+72 ) FC-1395 Product - 39 reate e 930 nom ' 770 - 160 (-17 %) 1 0 +1 0 European Fabric Protector 60 ND -60 Product European Fabric Protector ND ND 0 Treated Web SG Carnet Protector Product rtrttrrt ND ND ND ND 0 0 Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Page 3 of 56 NMPaoegvmeeom2 ber 14, 2000 Table 2. Concentrations of N-Methyl FOS Amide (ppm) Sample Identification ANm-ideet(or1iginal! ANm- ideet(cu1rrent! 4101W Product 4101 Treated Web ND ND ND ND FC-1395 Product FC-1395 Treated Web 140 nnm N 150 nnm N European Fabric Protector Product European Fabric Protector Treated Web ND ND ND ND SG Carnet Protector Product SG Carpet Protector Treated W eb ND ND ND ND Difference 0 0 0( 0 0 0 ) 0 0 Table 3. Concentrations of N,N-Dimethyl FOS Amide (ppm) Sample Identification 4101W Product 4101 Treated Web FC-1395 Product FC-1395 Treated Web European Fabric Protector Product European Fabric Protector Treated Web SG Carnet Protector Product SG Carpet Protector Treated W eb NF,N(Oo-rSigAiinmaitld!e 1 32 ND 0 N ND ND ND ND NF,NO(c-uSrAiremnittd! e 1 ND ND 560 nnm N ND ND ND ND Difference 0 -210 nnm 1-27 %! 0 0 0 0 0 Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Page 4 of 56 NMPaoegvmeeom3 ber 14, 2000 Table 4. Concentrations of PFOS (ppm) Sample Identification 4101W Product 4101 Treated Web FC-1395 Product FC-1395 Treated Web European Fabric Protector Product European Fabric Protector Treated Web SG Carnet Protector Product SG Carpet Protector Treated W eb PFOS (ori inai) ND <5 <5 ND ND <5 220 200 PFOS (c rrent) 2 11 27 <1 <1 2 610 470 Difference _____ +6 +22 _____ -- -- +390 (+180 %) +2 0(+140 ) Table 5. Concentrations of PFHS (ppm) Sample Identification 4101W Product 4101 Treated Web FC-1395 Product FC-1395 Treated Web European Fabric Protector Product European Fabric Protector Treated Web SG Carnet Protector Product SG Carpet Protector Treated W eb PF S (ori inai) ND <5 ND ND ND <5 440 270 PF S (c rrent) 1.2 1 .0 ND ND ND 2.6 1.900 1,900 Difference +1.2 _____ _____ _____ -- -- +1.500 (+340 %) +1,600 (+590 ) Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Page 5 of 56 NMPaoegvmeeom4 ber 14, 2000 Table 6. Concentrations of FOS Amide (ppm) Sample Identification 4101W Product 4101 Treated Web FC-1395 Product FC-1395 Treated Web European Fabric Protector Product European Fabric Protector Treated Web SG Carnet Protector Product SG Carpet Protector Treated W eb F(OorSigAinmaild)e ND <5 <5 ND ND <5 ND ND F(OcuSrAremnitd) e 2.5 1.0 110 ND ND ND 6.0 ND Difference _____ +6 +100 _____ -- -- +6 0 Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Page 6 of 56 Appendix A2: 2nd Test Sample Re-analysis TO: John Butenhoff 3M October 28, 2003 FROM: FCrAedTLC. -DCeRhroooms;at2o0g1r-a1pWhy-2G9r;o6u-p0665 REGARDING: RPerqoudeuscttGs aIDnd-7i8n88T9r,eDateetderFmaibnraiction of Fluorochemical Residuals in Introduction EmFpbmadfsslahmeeeOiueemgorftottiefSohwhhdlprrrEutoaeomenloec,McsdiasarrhimsnnyonmAewetlheamFhadp)tlee,thayilixregaetcdyseyrusiawil(eslamrMlwFeleslorseOirueeeors1ettrslhtS.hefeeiauyodxydnTcsslnutlaehurhiiFaFaldcnlbelyOocOsmtazftr,sSheoiScepidsedridtEepatde.tecamMet,hdoi(tcTefetPiAiaitdsfdhFfchcoeieee)Fychr,s,t1aalteeamaerAnldrmFentlmahy)eOaad.lptytylimhalSeynlseTytdseeFeachliistOsdfhitehFaafaeltySObobsortnlhterlrStraeiidFeiucddcfsyeOascir.eetcymt,ecsSwualTcromaitrmeradihnetioreecesebtientarn,eh(oypiseeeEdnylrfxtaaeotlhitihtntdfhdnrFyeydateulOphtlc(chtFMeeftFShteelryOsuOefdeafloSwctSucolbhrocooleEoeyaolnnrrocfmAlhteoochawFoo)ierieed,leOnicnimneetteotSahyg,rtdirnhacelEdpteayarssiAiatleleloudhyrtm)nrFliaysh,felsgiOlyuoimssvrnltoFi.SaedoeiFOfpdcultTmaOthhSahhwatysleiSeces.llatieihttlFdaaohacamnOforc(aaghPiriSlnedyosyFtteollteOa.,ialrctvnSFeeaTeas)Oln(thlEyoSteatrtrehesyl Sample Preparation mtTaarchagLeneltasoolsfinfesqiratuarrceiuiedldtetoos.tsnaoamTimtgrhplipealleelsem.ssriwaTvxuihettaureoler.semadsTmiiwxlhupteeutleerrwedreesvcpbeiwranisloetsarrr.mieftoupsghlaeeansdka,welynaesnroidesveetbhxrynetrnmigacaihxnttei,ndaaglnbiqdy1utgmohrteiaxonmifnatolghifqe0tu.hc5oleetgsasraroamlmfitqpshuleeoidfewxtwhittraehaswc5ttresmabwnLwseforieetfrhre5d to Analytical Method for the Determination of Semivolatile Fluorochemical Residuals Twdeospaifhiacltteimhhhtlfeeefallustntaeasoamrrrlagyoaetetnce5tihdo0awenanmieaCz0rila.ecy2wtati5aeloinstnrphaeimldsnayiedztlftheiueineldcametlstiaooacrthmnoptinerpc(mcoGkleednpCnsueet/cs(rrFfeasaaIttDwisucottr)aaone.nsrserdAuawdasmree3fdropd0erc-foumddofreirevl1tuttee0hetrsiremocDCnsione)/Bvm.pee-ad5irrniaunctusgoiipolntunhgtmose.gn2eaT7xsw0hpcieeothhcCrst.oaeammdS0tapc.a3tlonoe2ndgscmarwearmndpethrrseiyaontlisi(unoTitjdnIiePeocrnta6esn8dg9oe0fs) Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Page 7 of 56 RPOaecgptoeobr2ter 28, 2003 Analytical Method for the Determination of PFOS and PFOA Residuals Tcco4ahacphmrereerotrmioamPentdFeaMitOdtoroeiuSiglrntecrauaktinhpsndLiehnwyiPngc/aeFhetaglerOneoarcAtCAtitovrAgwoe9iRsel5iperoTner%natLidyMziaeacictotoheeindrrotmoioenzslniapmniLtthieroCoeiddlnr/eeMeum.si1nSian0TDsw0ghsaeFRcst1oepsPPmare-Lmc8obtCvrpicnoe/loMeermlduaScemohtprsmineyygrpshwi(tooFepidnm1tehePornLfeaftoqCsm1ru/m5wEiopmaeSbpnrIiieel-cneMdeuspewtSlephi)qisaa.t.uhrsaiTedathegnedrdaaudEnsiSaienlInygtssooaeusf1rw1c20e5er%me m x O il .C H 3 c 8f 17-- s - n O CH3 Dimethyl FOS Amide O C 2H5 c 8Fi -- s - n O C2H5 Diethyl FO S Am ide C 8F i 7 O II ,H S -N O CH3 Methyl FOS Amide C 8F l7 0 O ,C H 3 ' ------\ Cl Methyl FOS Chloride O II C2H 5 C 8F i7-- S - N 8 17 II O OH Ethyl FO S Alcohol O c h 33 C 8F i7-- S -- N 8 17 II O O O Methyl FOS Acrylate C 8F i 7- O II /H S -N O C 2H5 Ethyl FO S Am ide O II 4 C 8F17-- S - N 8 17 II O FOS Amide O C 2 H5 C 8F i7-- S -- N 8 17 II O Cl Ethyl FO S Chloride O II C 2H5 C 8F i7-- S -- N 8 17 II O O Ethyl FO S Acrylate O O II C H 3 C8Fi 7-- s - n O --\ OH Methyl FOS Alcohol O /CH3 C 8Fi7-- S -- N 8 17 II \ O ' ----- \ _VO O H3C Methyl FOS Methacrylate O II zC 2H5 C 8 Fi7-- S -- N 8 17 II ' O O O H3C Ethyl FO S Methacrylate O II C 8F17-- S - O H 8 17 II O PFOS O II C 6 H i3-- S --OH 6 13 II O PFHS Figure 1. Fluorochemical Residuals of Interest Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Page 8 of 56 RPOaecgptoeobr3ter 28, 2003 pTahretsr-epseurl-tms iflrloiomnt(hpepamn)aloyrsepsg/agrreamsumofmmaaritzeeridali.n Table 1. The concentrations are reported in units of Table 1. Analytical Results (parts-per-million) Analyte T- 7088.2 T-7338 T- 7090.2 T-7339 T-7340 T-7341 T-7342 T-7343 Dimethyl FOS Amide 16 <10 <5 <10 430 <10 <5 <10 Methyl FOS Amide 160 <10 7.7 <10 180 <10 <5 <10 Ethyl FOS Amide 8.9 <10 19 <10 <5 <10 <5 <10 FOS Amide 28 <10 <5 <10 350 <10 <5 <10 Diethyl FOS AMIDE <5 <10 12 <10 <5 <10 <5 <10 Methyl FOS Chloride 13 <10 <5 <10 140 <10 <5 <10 Ethyl FOS Chloride <5 <10 14 <10 <5 <10 <5 <10 Methyl FOS Alcohol 5.000 1.600 1.100 <10 4.000 180 47 <10 Ethyl FOS Alcohol 16 1 2,600 4,800 2 <10 <5 <10 ethyl FOS Acrylate 12 <10 5 10 <5 <10 <5 <10 Ethyl FOS Acrylate 8.9 <10 18 <10 200 <10 <5 <10 ethyl FOS ethacrvlate 20 12 <5 <10 54 <10 <5 <10 thyl FOS ethacrvlate <5 <10 21 32 38 <10 <5 <10 PFOS 1.0 8.6 <0.5 <1 1.6 <1 180 190 FS .. .. . . Toorfhes1e0dpepatpreamctetioofnnluaolirmwoecitihsgehfmot/riwctaheleigrsehestmibdaiuvsaoilssl.a,twiSleaitmhflputhloeersohTcihg-7eh0me8sit8c.ca2lonraecnsedindTtur-aa7lt3iod4ne0terceromsinditnuaaiantliseodnbsethiwnegegrmreeeaettihethysetlrnF5uOmpSpbmer twashalecemroedphelofetolesucawntnidodenrteeotlheicmyiotlhintFet,arOwilnSohwisacilghconorwihfbaioecslla.o1nw0tScaptomhpnempcldeefenotTterr-cat7htti3iioos4nn3ssaldimomifdpiPtln.eFo.Ot TScowanontadoinfPtFahHneySsf.almuTophrleoecsl,heveTme-l7si3ci4anl2trhaenesiddoutTha-el7sr3a4b3ove tTahnheeofnisn-asamiltperelvepeopnrrtde.oprareamtipolnoysteeep.sFarneddtLhe. DGeCRaonods HdiPreLcCte/dMtSheawnaolryks,esinwteerrperectaerdritehdeoduattabyanMdipkreepBaursecdh, F.L. DeRoos Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Page 9 of 56 Appendix B: Clinical Observation Data Tablel: Clinical Summary Incidence Report 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll su b-se ts in Sprague-Dawley Rats C 1 1 n 1 c a 1 S u mma r y I n c i d e n c e R e p o r t C l i n . Ob se rvat ion Summary A-E Page : 1 (Brief report) Days -6 - 42 With reference to nominal day zero Dose Group Dose Total animals A Control 16 B l.Test art. 16 c 2.Test art. 16 D 3.Test art. 16 E 4.Test art. 16 GENOBS WITHIN NORMAL LIMITS Incid ence (%) 16(100) 16(100) 16(100) 16(100) 16(100) SKIN SCABBY SKIN Max Score 3 Incid ence (%) Mean S e v e r i t y TOTALS Incid ence (%) 0( 0) 0( 0) 0( 0) K 6) 2.0 K 6) 0( 0) O oo o OO oo oo oo OO oo oo oo OO oo oo oo 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F r a u n h o f e r - In s t . f . T o x i k o l o g ie u. Aerosol fo r s c h . Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats C 1 1 n 1 c a 1 S u mma r y I n c i d e n c e R e p o r t C l i n . Observ ation Summary F - I Page : 2 (Brief report) Days -6 - 42 With reference to nominal day zero Dose Group Dose Total animals F 5.Test art. 16 G 6.Test art. 16 H 7.Test art. 16 I 8.Test art. 16 GENOBS WITHIN NORMAL LIMITS Incid ence (%) 16(100) 16(100) 16(100) 16(100) SKIN SCABBY SKIN Max Score 3 Incid ence (%) Mean S e v e r i t y TOTALS Incid ence (%) 0( 0) 0( 0) 0( 0) K 6) 1.0 K 6) Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 10 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Table 2: Individual Data on Clinical Observation 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study 01G00002 Species Rat Sex Both All sub-sets Days -6 to 42 in Sprague-Dawley Rats C 1 i n i c a 1 Co mp r e s s e d H i s t o r y Re p o r t Group: A An ima 1 Obse rvat ion Days 010001 GENERAL OBSERVATION WITHIN NORMAL L IM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 010002 GENERAL OBSERVATION WITHIN NORMAL L IM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 010003 GENERAL OBSERVATION WITHIN NORMAL L IM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 010004 GENERAL OBSERVATION WITHIN NORMAL L IM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 010005 GENERAL OBSERVATION WITHIN NORMAL L IM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 010006 GENERAL OBSERVATION WITHIN NORMAL L IM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 010007 GENERAL OBSERVATION WITHIN NORMAL L IM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) 010008 GENERAL OBSERVATION WITHIN NORMAL L IM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) Page : 1 Dose: Control Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 11 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: B An 1ma 1 Observatio n Days 030001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 030002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 030003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 030004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 030005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 030006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 030007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) 030008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) Page : 2 Dose: l.T e st a r t. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 12 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: C An 1ma 1 Observatio n Days 050001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 050002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 050003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 050004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 050005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 050006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 050007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) 050008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) Page : 3 Dose: 2 .Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 13 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: D An 1ma 1 Observatio n Days 070001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 070002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 070003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 070004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 070005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 070006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 070007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) 070008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) Page : 4 Dose: 3 .Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 14 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: E An 1ma 1 Observatio n Days 090001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 090002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 090003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 090004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 090005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 090006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 090007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) 090008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) Page : 5 Dose: 4 .Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 15 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: L An 1ma 1 Observatio n Days 110001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 110002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 110003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 110004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 110005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 110006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 110007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) 110008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) Page : 6 Dose: 5.Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 16 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1 n i c a 1 C o mp r e s s e d H 1 s t o r y R e p o r t Group: G An 1ma 1 Observatio n Days 130001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 130002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 130003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 130004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 130005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 130006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 130007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) 130008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) Page : 7 Dose: 6 .Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 17 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: H An 1ma 1 Observatio n Days 150001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 150002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 150003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 150004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 150005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 150006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 150007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) 150008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) Page : 8 Dose: 7.Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 18 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: I An 1ma 1 Observatio n Days 170001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 170002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0 4 (12-MAY-00) 170003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 170004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14 14 (22-MAY-00) 170005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 170006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28 28 (05-JUN-00) 170007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) 170008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -6,0,7,14,21,28,36,42 42 (19-JUN-00) Page : 9 Dose: 8 .Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 19 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: A An 1ma 1 Observatio n Days 020003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 020004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 020005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 020006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 020007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) 020008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) Page : 10 Dose: Control Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 20 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: B An 1ma 1 Observatio n Days 040003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 040004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 040005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 040006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 040007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) 040008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) Page : 11 Dose: l.T e s t a r t. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 21 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: C An 1ma 1 Observatio n Days 060003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 060004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 060005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 060006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 060007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) 060008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) Page : 12 Dose: 2 .Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 22 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 i n i c a 1 Co mp r e s s e d H i s t o r y Re p o r t Group: D An ima 1 Observatio n Days 080003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 080004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 080005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) SKIN SCABBY SKIN LEFT FORELEG MEDIUM B0TH-F0RELEG MEDIUM -1,6,13,20 28 (06-JUN-00) 27 28 080006 GENERAL OBSERVATION WITHIN NORMAL L IM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 080007 GENERAL OBSERVATION WITHIN NORMAL L IM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) 080008 GENERAL OBSERVATION WITHIN NORMAL L IM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) Page : 13 Dose: 3 .Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 23 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: E An 1ma 1 Observatio n Days 100003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 100004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 100005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 100006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 100007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) 100008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) Page : 14 Dose: 4 . Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 24 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: L An 1ma 1 Observatio n Days 120003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 120004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 120005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 120006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 120007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) 120008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) Page : 15 Dose: 5.Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 25 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: G An 1ma 1 Observatio n Days 140003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 140004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 140005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 140006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 140007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) 140008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) Page : 16 Dose: 6.Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 26 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: H An 1ma 1 Observatio n Days 160003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 160004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 160005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 160006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 160007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) 160008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) Page : 17 Dose: 7.Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 27 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: I An 1ma 1 Observatio n Days 180003 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 180004 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13 14 (23-MAY-00) 180005 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 180006 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20 28 (06-JUN-00) 180007 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -1,6,13,20,32,35,41 42 (20-JUN-00) 180008 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) SKIN SCABBY SKIN LEFT FORELEG SLIGHT with text -1,6,13,32,35,41 42 (20-JUN-00) 20,27 Page : 18 Dose: 8 .Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 28 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1 n i c a 1 C o mp r e s s e d H 1 s t o r y R e p o r t Group: A An 1ma 1 Observatio n Days 020001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) 020002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) Page : 19 Dose: Control Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 29 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: B An 1ma 1 Observatio n Days 040001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) 040002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) Page : 20 Dose: l.T e st a r t. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 30 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: C An 1ma 1 Observatio n Days 060001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) 060002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) Page : 21 Dose: 2 .Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 31 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: D An 1ma 1 Observatio n Days 080001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) 080002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) Page : 22 Dose: 3 .Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 32 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: E An 1ma 1 Observatio n Days 100001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) 100002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) Page : 23 Dose: 4 .Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 33 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: L An 1ma 1 Observatio n Days 120001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) 120002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) Page : 24 Dose: 5.Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 34 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: G An 1ma 1 Observatio n Days 140001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) 140002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) Page : 25 Dose: 6.Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 35 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: H An 1ma 1 Observatio n Days 160001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) 160002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) Page : 26 Dose: 7.Test art. Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 36 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets Days : -6 to 42 in Sprague-Dawley Rats C 1 1n i c a 1 Co mp r e s s e d H 1 s t o r y Re p o r t Group: I An 1ma 1 Observatio n Days 180001 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) 180002 GENERAL OBSERVATION WITHIN NORMAL LIM IT S KILLED (SCHEDULED) -3,4 4 (15-MAY-00) Page : 27 Dose: 8 .Test art. * * * END OF REPORT * * * Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 37 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Appendix C: Individual and Mean Body Weight Data 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We i g h t [ g ] MA L E S Animal No. Day -6 Day 0 Day 3 Day 7 Day 10 Day 14 Control Control l.Test art. l.Test art. 2.Test art. 2.Test art. 3.Test art. 3.Test art. A 010001 A 010002 A 010003 A 010004 A 010005 A 010006 A 010007 A 010008 Mean SD n B 030001 B 030002 B 030003 B 030004 B 030005 B 030006 B 030007 B 030008 Mean SD n C 050001 C 050002 C 050003 C 050004 C 050005 C 050006 C 050007 C 050008 Mean SD n D 070001 D 070002 D 070003 D 070004 D 070005 D 070006 D 070007 D 070008 Mean SD n 264.8 262.2 248.2 259.9 263.2 256.3 258.0 270.1 260.3 6.5 8 259.3 255.1 260.8 261.9 259.6 238.9 253.9 258.1 256.0 7.4 8 257.8 249.7 254.3 253.9 272.5 242.4 253.1 268.9 256.6 9.9 8 260.6 268.5 258.4 267.1 266.5 248.5 253.3 261.5 260.6 7.0 8 294.1 289.3 278.2 280.0 288.2 281.1 280.3 297.4 286.1 7.2 8 285.1 284.4 284.7 280.2 291.1 255.0 278.2 280.4 279.9 10.8 8 277.5 276.6 279.8 277.9 301.7 261.6 271.7 297.3 280.5 13.1 8 285.9 300.4 277.1 268.9 292.3 274.3 275.7 292.1 283.3 11.0 8 293.6 294.0 275.7 282.9 285.3 281.5 268.3 300.6 285.2 10.6 8 290.6 285.3 276.0 287.2 292.5 264.2 280.1 279.1 281.9 9.2 8 282.3 279.8 262.2 274.1 309.1 265.0 274.7 298.9 280.8 16.1 8 283.7 307.9 271.0 273.0 290.1 278.3 275.9 294.2 284.3 12.5 8 278.2 289.6 296.1 296.6 269.0 310.1 289.9 14.6 6 282.3 295.2 302.3 267.9 289.4 285.8 287.2 11.8 6 274.4 283.4 321.5 274.0 279.6 308.3 290.2 19.9 6 273.5 280.3 298.1 290.2 284.7 301.3 288.0 10.6 6 280.8 304.6 303.7 304.0 278.7 321.1 298.8 16.2 6 294.2 296.6 307.0 274.7 291.9 288.1 292.1 10.6 6 283.2 289.4 328.7 283.6 291.1 319.9 299.3 19.8 6 283.7 290.2 301.0 293.7 288.7 307.9 294.2 8.8 6 289.0 311.7 304.4 311.8 287.7 328.6 305.5 15.5 6 300.5 306.9 317.4 280.4 300.0 300.0 300.9 12.1 6 309.7 293.1 313.5 293.0 303.3 334.9 307.9 15.7 6 289.1 298.8 315.1 306.9 300.2 313.5 303.9 9.9 6 S t a t i s t i c s : Anova + Dunnett's t e s t s . (Two-Si ded) * p<=5% ; * * p<=i% ; Page : 1 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 38 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We i g h t [ g ] MA L E S 4.Test art. 4.Test art. 5.Test art. 5.Test art. 6.Test art. 6.Test art. 7.Test art. 7.Test art. Animal No. Day -6 E 090001 E 090002 E 090003 E 090004 E 090005 E 090006 E 090007 E 090008 265.8 262.0 246.2 256.0 262.4 245.1 249.4 265.1 Mean SD n 256.5 8.6 8 F 110001 F 110002 F 110003 F 110004 F 110005 F 110006 F 110007 F 110008 266.9 258.2 239.1 244.3 270.1 240.9 249.0 264.6 Mean SD n 254.1 12.4 8 G 130001 G 130002 G 130003 G 130004 G 130005 255.4 262.7 249.5 255.2 261.7 G 130006 G 130007 G 130008 233.9 251.9 268.3 Mean SD n 254.8 10.5 8 H 150001 H 150002 H 150003 H 150004 H 150005 H 150006 H 150007 H 150008 259.3 260.4 252.0 254.4 261.4 243.2 242.2 261.0 Mean SD n 254.2 7.9 8 Day 0 293.3 289.3 264.5 286.3 288.6 272.8 274.6 306.7 284.5 13.4 8 296.0 283.8 250.0 267.3 302.0 268.6 278.7 295.2 280.2 17.6 8 288.8 297.8 284.2 284.7 293.6 256.8 281.9 297.4 285.7 13.1 8 278.9 280.4 279.2 284.2 284.7 273.8 262.5 284.5 278.5 7.5 8 Day 3 288.6 297.0 264.9 282.5 299.7 278.8 285.3 313.2 288.8 14.7 8 302.8 290.3 247.6 267.3 296.9 273.4 279.1 292.7 281.3 18.2 8 289.7 303.9 288.2 294.9 298.8 258.8 284.2 300.2 289.8 14.2 8 288.6 273.6 287.9 276.9 288.9 280.8 267.0 288.1 281.5 8.3 8 Day 7 Day 10 Day 14 275.0 287.9 302.8 289.4 291.9 328.6 295.9 18.3 6 279.5 295.6 312.6 294.6 301.3 337.7 303.6 19.9 6 281.8 297.3 318.8 306.2 317.2 354.2 312.6 24.6 6 245.6 272.4 308.1 287.5 284.9 306.1 284.1 23.2 6 253.0 280.5 316.5 293.8 295.1 317.0 292.7 24.0 6 256.9 295.0 326.6 291.8 300.4 325.2 299.3 25.7 6 297.1 302.6 309.8 267.5 295.0 313.6 297.6 16.4 6 303.1 307.7 319.9 275.5 295.8 319.4 303.6 16.6 6 316.2 313.5 323.5 277.2 309.5 337.4 312.9 20.0 6 299.9 282.0 297.3 293.2 272.5 294.4 289.9 10.5 6 304.2 282.1 303.9 298.8 274.7 296.0 293.3 12.2 6 308.7 302.7 314.3 314.3 283.1 306.0 304.9 11.6 6 S t a t i s t i c s : Anova + Dunnett's tests. (Two-Si ded) * p<=5% ; * * p<=i% ; Page : 2 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 39 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsch Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We i g h t [ g ] MA L E S 8.Test art. 8.Test art. Animal No. Day -6 I 170001 I 170002 I 170003 I 170004 I 170005 I 170006 I 170007 I 170008 262.9 251.5 259.4 245.8 257.2 249.4 255.0 268.1 Mean SD n 256.2 7.3 8 Day 0 296.5 280.1 291.7 272.4 281.9 269.7 283.3 300.3 284.5 10.9 8 Day 3 300.6 284.6 285.4 281.7 280.3 268.7 285.7 308.2 286.9 12.3 8 Day 7 Day 10 Day 14 296.7 295.1 291.2 275.5 300.5 318.7 296.3 14.0 6 301.4 304.7 298.5 279.8 302.7 331.3 303.1 16.5 6 315.6 311.2 301.4 296.8 318.5 344.8 314.7 16.9 6 S t a t i s t i c s : Anova + Dunnett's t e s t s . (Two-Si ded) P<=5% P<=1% Page : 3 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 40 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We i g h t [ g ] F E MA L E S Animal No. Day -6 Day 0 Day 3 Day 7 Day 10 Day 14 Control Control l.Test art. l.Test art. 2.Test art. 2.Test art. 3.Test art. 3.Test art. A 020003 A 020004 A 020005 A 020006 A 020007 A 020008 A 020001 A 020002 Mean SD n B 040003 B 040004 B 040005 B 040006 B 040007 B 040008 B 040001 B 040002 Mean SD n C 060003 C 060004 C 060005 C 060006 C 060007 C 060008 C 060001 C 060002 Mean SD n D 080003 D 080004 D 080005 D 080006 D 080007 D 080008 D 080001 D 080002 Mean SD n 199.3 203.7 195.8 196.9 198.8 209.3 189.7 212.6 200.8 7.5 8 202.2 213.3 196.6 191.4 192.1 208.2 185.5 207.4 199.6 9.7 8 201.0 203.5 189.2 201.6 195.6 206.4 180.8 218.9 199.6 11.4 8 199.7 196.0 203.2 204.3 187.6 186.7 183.8 209.0 196.3 9.3 8 209.5 208.9 205.4 220.6 201.5 213.3 194.3 205.2 207.3 7.8 8 231.1 210.7 209.1 198.9 213.6 217.8 194.1 206.1 210.2 11.4 8 208.0 218.6 199.0 212.6 202.3 211.6 202.1 223.1 209.7 8.5 8 205.3 206.8 215.0 216.3 202.1 202.9 196.1 231.4 209.5 11.1 8 S t a t i s t i c s : Anova + Dunnett's tests. 226.1 214.9 212.6 214.7 202.8 217.1 198.7 214.6 212.7 8.5 8 233.1 211.6 216.1 207.0 210.6 219.9 213.1 221.0 216.6 8.2 8 215.1 224.5 197.0 218.6 203.3 214.6 192.3 219.2 210.6 11.6 8 210.6 211.5 219.9 219.7 205.6 201.6 205.6 229.2 213.0 9.3 8 236.8 221.2 215.0 226.0 210.2 224.5 222.3 9.3 6 232.3 220.3 222.8 209.2 214.0 228.3 221.2 8.6 6 219.0 232.3 204.4 230.2 211.7 224.5 220.4 10.8 6 226.1 229.0 223.3 229.9 217.9 217.4 223.9 5.4 6 246.4 229.2 217.7 227.6 214.1 230.7 227.6 11.4 6 237.6 223.6 228.9 217.9 205.8 236.3 225.0 12.0 6 221.2 236.4 207.9 238.3 227.1 226.7 226.3 11.1 6 236.7 241.8 248.0 232.3 214.3 230.2 233.9 11.6 6 264.0 236.5 224.3 234.2 216.9 238.2 235.7 16.1 6 247.3 235.1 234.1 220.9 216.7 247.6 233.6 12.9 6 233.3 241.5 212.0 243.7 229.2 235.5 232.5 11.4 6 242.0 243.4 253.1 250.5 224.3 227.4 240.1 11.9 6 (Two-Si ded) * p<=5% ; ** p<=l% ; Page : 4 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 41 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We 1 g h t [ g ] F E MA F E S Animal No. Day -6 4.Test art. E 100003 E 100004 E 100005 E 100006 E 100007 E 100008 E 100001 E 100002 204.5 210.1 189.2 198.8 186.6 204.4 194.8 203.7 4.Test art. Mean SD n 199.0 8.2 8 5.Test art. F 120003 F 120004 F 120005 F 120006 F 120007 F 120008 F 120001 F 120002 208.7 199.0 201.4 201.3 189.5 194.7 191.6 202.9 5.Test art. Mean SD n 198.6 6.4 8 6.Test art. G 140003 G 140004 G 140005 G 140006 G 140007 G 140008 G 140001 G 140002 211.9 205.8 198.9 217.6 185.4 204.7 204.2 209.1 6.Test art. Mean SD n 204.7 9.6 8 7.Test art. H 160003 H 160004 H 160005 H 160006 H 160007 H 160008 H 160001 H 160002 203.7 191.4 202.0 199.4 187.0 207.6 196.2 205.8 7.Test art. Mean SD n 199.1 7.2 8 Day 0 206.1 224.4 198.8 202.6 196.3 226.0 209.3 203.1 208.3 11.2 8 216.7 203.9 205.1 217.6 204.9 209.3 201.6 224.7 210.5 8.2 8 207.1 208.1 204.6 218.6 184.6 205.3 211.6 222.3 207.8 11.3 8 209.6 200.8 208.6 199.8 191.4 206.2 213.6 208.9 204.9 7.1 8 S t a t i s t i c s : Anova + Dunnett's tests. Day 3 Day 7 Day 10 Day 14 211.7 230.3 201.0 202.8 198.1 229.5 203.9 216.2 211.7 12.7 8 215.2 207.5 209.0 216.8 205.6 217.6 205.1 229.7 213.3 8.3 8 217.6 206.3 205.7 219.4 191.3 216.9 222.2 229.2 213.6 11.9 8 216.4 206.2 207.5 213.1 195.5 201.7 211.1 216.4 208.5 7.3 8 220.9 235.4 209.3 206.9 210.1 238.6 220.2 13.9 6 237.4 222.2 216.1 225.5 212.1 231.1 224.1 9.4 6 223.2 222.2 214.6 229.3 205.9 227.9 220.5 8.8 6 230.9 212.7 226.1 222.2 205.1 212.1 218.2 9.8 6 226.0 239.2 203.5 211.7 208.2 238.4 221.2 15.6 6 248.0 225.2 222.3 234.2 220.9 228.5 229.9 10.1 6 220.3 217.1 221.9 226.6 204.7 227.5 219.7 8.3 6 227.5 210.7 229.8 222.0 206.6 213.4 218.3 9.5 6 231.3 243.4 219.1 220.1 216.1 253.5 230.6 15.1 6 251.4 227.4 238.0 236.7 227.8 240.3 236.9 8.9 6 226.8 222.8 229.3 233.3 209.7 235.5 226.2 9.3 6 240.8 217.4 234.5 231.7 211.3 221.5 226.2 11.3 6 (Two-Si ded) * p<=5% ; * * p<=i% ; Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 42 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We i g h t [ g ] F E MA L E S Animal No. Day -6 8.Test art. I 180003 I 180004 I 180005 I 180006 I 180007 I 180008 I 180001 I 180002 192.9 203.1 197.2 199.0 193.6 212.0 194.3 213.2 8.Test art. Mean SD n 200.7 8.1 8 Day 0 200.3 212.6 203.4 208.7 202.3 240.4 207.1 229.0 213.0 14.3 8 Day 3 211.2 212.9 206.5 215.3 205.2 241.6 204.4 235.7 216.6 14.2 8 Day 7 Day 10 Day 14 225.8 223.5 210.3 228.2 216.7 251.4 219.9 229.3 219.2 233.8 215.2 252.2 225.4 236.8 222.0 234.2 233.6 266.5 226.0 14.1 6 228.3 13.6 6 236.4 15.8 6 S t a t i s t i c s : Anova + Dunnett's t e s t s . (Two-Si ded) * P<=5% . * * p<=l% ; Page : 6 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 43 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We i g h t [ g ] MA L E S Animal No. Day 17 Day 21 Day 24 Day 28 Day 31 Day 35 Control Control l.Test art. l.Test art. 2.Test art. 2.Test art. A 010005 A 010006 A 010007 A 010008 Mean SD n B 030005 B 030006 B 030007 B 030008 Mean SD n C 050005 C 050006 C 050007 C 050008 Mean SD n 310.6 329.4 285.9 334.8 315.2 22.1 4 318.2 280.9 304.8 305.8 302.4 15.6 4 333.0 300.4 305.6 340.0 319.8 19.7 4 316.2 329.1 297.5 343.5 321.6 19.5 4 323.5 276.5 312.1 332.4 311.1 24.5 4 340.3 304.3 309.8 343.7 324.5 20.4 4 323.0 326.7 287.5 344.3 320.4 23.8 4 325.2 279.8 318.6 318.9 310.6 20.8 4 356.9 311.5 315.1 347.2 332.7 22.8 4 324.9 338.3 294.2 354.7 328.0 25.6 4 337.1 288.0 326.8 327.0 319.7 21.7 4 367.4 314.6 329.5 354.2 341.4 23.8 4 315.2 365.9 340.6 35.9 2 338.2 325.9 332.1 8.7 2 338.2 369.8 354.0 22.3 2 320.8 365.4 343.1 31.5 2 337.7 342.0 339.9 3.0 2 346.4 378.1 362.3 22.4 2 3.Test art. 3.Test art. 4.Test art. 4.Test art. 5.Test art. 5.Test art. D 070005 D 070006 D 070007 D 070008 Mean SD n E 090005 E 090006 E 090007 E 090008 Mean SD n F 110005 F 110006 F 110007 F 110008 Mean SD n 320.1 311.3 302.6 322.4 314.1 9.0 4 328.8 318.8 323.5 358.6 332.4 17.9 4 322.9 306.3 308.0 333.4 317.7 12.9 4 322.5 314.9 314.4 326.5 319.6 5.9 4 329.4 318.9 323.8 362.1 333.6 19.5 4 336.0 310.2 310.2 343.1 324.9 17.2 4 S t a t i s t i c s : Anova + Dunnett's tests. 321.5 315.6 316.8 326.9 320.2 5.1 4 333.5 315.3 329.8 367.6 336.6 22.1 4 329.4 315.9 315.4 347.7 327.1 15.2 4 332.1 328.9 329.3 336.2 331.6 3.4 4 336.4 329.1 338.8 378.0 345.6 22.0 4 339.5 328.0 322.3 355.8 336.4 14.8 4 343.7 350.1 346.9 4.5 2 356.7 394.6 375.7 26.8 2 336.6 367.0 351.8 21.5 2 348.5 352.1 350.3 2.5 2 360.3 402.0 381.2 29.5 2 347.4 376.6 362.0 20.6 2 (Two-Si ded) * p<=5% ; * * p<=l% ; Page : 7 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 44 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We i g h t [ g ] MA L E S 6.Test art. 6.Test art. 7.Test art. 7.Test art. 8.Test art. 8.Test art. Animal No. Day 17 G 130005 G 130006 G 130007 G 130008 327.2 282.7 308.8 344.7 Mean SD n 315.9 26.5 4 H 150005 H 150006 H 150007 H 150008 311.3 318.2 283.5 303.0 Mean SD n 304.0 15.0 4 I 170005 I 170006 I 170007 I 170008 303.0 292.4 316.9 336.7 Mean SD n 312.3 19.1 4 Day 21 338.7 284.9 315.3 346.1 321.3 27.6 4 311.9 322.5 286.0 309.2 307.4 15.4 4 307.8 305.7 320.1 346.7 320.1 18.9 4 Day 24 329.9 290.8 313.7 346.9 320.3 23.9 4 316.7 325.5 293.0 311.7 311.7 13.7 4 313.6 311.6 327.9 345.1 324.6 15.5 4 Day 28 341.9 298.2 324.1 355.3 329.9 24.7 4 323.9 330.9 301.6 325.3 320.4 12.9 4 328.3 320.1 334.9 361.4 336.2 17.9 4 Day 31 333.7 368.6 351.2 24.7 2 310.4 338.4 324.4 19.8 2 346.5 374.0 360.3 19.4 2 Day 35 334.8 374.8 354.8 28.3 2 314.6 338.1 326.4 16.6 2 359.4 382.3 370.9 16.2 2 S t a t i s t i c s : Anova + Dunnett's tests. (Two-Si ded) * p<=5% ; * * p<=l% ; Page : 8 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 45 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We i g h t [ g ] F E MA L E S Animal No. Day 17 Day 21 Day 24 Day 28 Day 31 Day 35 Control Control l.Test art. l.Test art. 2.Test art. 2.Test art. A 020005 A 020006 A 020007 A 020008 Mean SD n B 040005 B 040006 B 040007 B 040008 Mean SD n C 060005 C 060006 C 060007 C 060008 Mean SD n 228.1 243.4 219.2 237.0 231.9 10.6 4 238.6 222.5 217.7 255.9 233.7 17.3 4 211.1 240.9 238.3 237.7 232.0 14.0 4 228.8 250.2 220.8 236.3 234.0 12.5 4 234.0 232.3 227.3 247.9 235.4 8.8 4 219.9 250.7 228.9 239.2 234.7 13.3 4 230.4 254.0 224.0 243.3 237.9 13.4 4 241.9 226.6 221.0 247.1 234.2 12.4 4 230.9 242.0 236.5 241.7 237.8 5.2 4 232.9 259.1 224.3 248.8 241.3 15.6 4 250.2 234.6 228.8 257.7 242.8 13.4 4 232.7 247.6 246.0 252.2 244.6 8.4 4 229.0 252.4 240.7 16.5 2 236.9 267.0 252.0 21.3 2 248.0 258.4 253.2 7.4 2 231.2 262.0 246.6 21.8 2 237.8 269.2 253.5 22.2 2 246.2 256.4 251.3 7.2 2 3.Test art. 3.Test art. 4.Test art. 4.Test art. 5.Test art. 5.Test art. D 080005 D 080006 D 080007 D 080008 Mean SD n E 100005 E 100006 E 100007 E 100008 Mean SD n F 120005 F 120006 F 120007 F 120008 Mean SD n 250.4 245.5 223.4 228.5 237.0 13.0 4 224.0 218.0 221.4 254.8 229.6 17.0 4 241.7 242.8 230.9 238.2 238.4 5.4 4 262.0 249.8 232.2 237.4 245.4 13.3 4 233.5 214.3 227.6 260.6 234.0 19.5 4 239.9 249.1 239.7 247.9 244.2 5.0 4 S t a t i s t i c s : Anova + Dunnett's tests. 265.2 245.8 227.6 231.7 242.6 17.0 4 230.6 214.0 229.8 262.6 234.3 20.4 4 250.5 256.6 238.2 247.3 248.2 7.7 4 271.8 253.8 232.5 242.5 250.2 16.9 4 234.9 218.9 233.4 270.7 239.5 22.0 4 254.2 254.8 244.4 252.8 251.6 4.8 4 240.3 261.1 250.7 14.7 2 237.0 277.2 257.1 28.4 2 246.4 254.4 250.4 5.7 2 240.7 269.8 255.3 20.6 2 244.2 278.6 261.4 24.3 2 250.4 260.7 255.6 7.3 2 (Two-Si ded) * p<=5% ; * * p<=l% ; Page : 9 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 46 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We i g h t [ g ] F E MA L E S Animal No. Day 17 6.Test art. G 140005 G 140006 G 140007 G 140008 231.2 238.0 216.8 236.4 6.Test art. Mean SD n 230.6 9.6 4 7.Test art. H 160005 H 160006 H 160007 H 160008 239.3 234.0 211.7 224.3 7.Test art. Mean SD n 227.3 12.1 4 8.Test art. I 180005 I 180006 I 180007 I 180008 225.1 239.2 232.9 265.6 8.Test art. Mean SD n 240.7 17.6 4 Day 21 237.2 247.1 228.7 249.3 240.6 9.5 4 249.7 241.9 235.7 237.8 241.3 6.2 4 230.2 243.9 237.9 269.4 245.4 17.0 4 Day 24 242.8 240.3 224.5 244.8 238.1 9.3 4 254.3 241.3 218.6 236.5 237.7 14.8 4 236.4 241.5 241.5 274.9 248.6 17.7 4 Day 28 244.0 251.1 231.9 253.5 245.1 9.7 4 257.4 246.1 221.5 241.4 241.6 15.0 4 238.6 254.5 247.7 281.7 255.6 18.6 4 Day 31 239.8 250.8 245.3 7.8 2 222.4 249.3 235.9 19.0 2 249.7 288.2 269.0 27.2 2 Day 35 240.3 249.3 244.8 6.4 2 229.7 258.5 244.1 20.4 2 255.2 288.2 271.7 23.3 2 S t a t i s t i c s : Anova + Dunnett's tests. (Two-Si ded) * p<=5% ; * * p<=l% ; Page : 10 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 47 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We i g h t [ g ] MA L E S Animal No. Day 38 Day 42 Control Control l.Test art. l.Test art. 2.Test art. 2.Test art. 3.Test art. 3.Test art. 4.Test art. 4.Test art. 5.Test art. 5.Test art. 6.Test art. 6.Test art. 7.Test art. 7.Test art. 8.Test art. 8.Test art. A 010007 A 010008 Mean SD n B 030007 B 030008 Mean SD n C 050007 C 050008 Mean SD n D 070007 D 070008 Mean SD n E 090007 E 090008 Mean SD n F 110007 F 110008 Mean SD n G 130007 G 130008 Mean SD n H 150007 H 150008 Mean SD n I 170007 I 170008 Mean SD n 324.1 374.8 349.5 35.8 2 348.5 347.6 348.1 0.6 2 354.8 383.0 368.9 19.9 2 358.0 371.0 364.5 9.2 2 362.1 407.3 384.7 32.0 2 356.9 377.1 367.0 14.3 2 338.9 377.8 358.4 27.5 2 320.8 343.5 332.2 16.1 2 365.1 389.7 377.4 17.4 2 336.7 378.0 357.4 29.2 2 352.3 357.2 354.8 3.5 2 361.0 390.4 375.7 20.8 2 363.7 383.6 373.7 14.1 2 374.2 412.9 393.6 27.4 2 368.1 384.1 376.1 11.3 2 348.4 385.2 366.8 26.0 2 330.0 347.4 338.7 12.3 2 375.2 399.5 387.4 17.2 2 S t a t i s t i c s : Anova + Dunnett's tests. (Two-Sided) * P<=5% ; ** P<=1% ; Page : 11 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 48 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats B o d y We i g h t [ g ] F E MA L E s Animal No. Day 38 Day 42 Control Control l.Test art. l.Test art. 2.Test art. 2.Test art. 3.Test art. 3.Test art. 4.Test art. 4.Test art. 5.Test art. 5.Test art. 6.Test art. 6.Test art. 7.Test art. 7.Test art. 8.Test art. 8.Test art. A 020007 A 020008 Mean SD n B 040007 B 040008 Mean SD n C 060007 C 060008 Mean SD n D 080007 D 080008 Mean SD n E 100007 E 100008 Mean SD n F 120007 F 120008 Mean SD n G 140007 G 140008 Mean SD n H 160007 H 160008 Mean SD n I 180007 I 180008 Mean SD n 232.6 261.6 247.1 20.5 2 234.2 269.7 252.0 25.1 2 245.5 259.5 252.5 9.9 2 237.9 256.6 247.3 13.2 2 251.1 279.7 265.4 20.2 2 254.8 259.9 257.4 3.6 2 244.5 261.4 253.0 12.0 2 224.8 257.3 241.1 23.0 2 246.0 290.6 268.3 31.5 2 233.6 258.1 245.9 17.3 2 243.0 271.2 257.1 19.9 2 248.1 260.8 254.5 9.0 2 245.5 256.4 251.0 7.7 2 250.6 281.0 265.8 21.5 2 261.9 260.7 261.3 0.8 2 245.4 260.2 252.8 10.5 2 217.6 254.5 236.1 26.1 2 259.8 298.6 279.2 27.4 2 S t a t i s t i c s : Anova + Dunnett's te st s. * * * END OF REPORT (Two-Sided) * P<=5% ; * * P<=1% Page : 12* Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 49 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats Appendix D: Absolute and Relative Liver Weights 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats T e r m i n a 1 B o d y w e i g 11 t & L i v e r W e i g h t D a t a MA L E S Control Control l.Test art. l.Test art. 2.Test art. 2.Test art. 3.Test art. 3.Test art. 4.Test art. 4.Test art. 5.Test art. 5.Test art. Animal No. Day 4 TermBW G A 010001 A 010002 A 010003 A 010004 295.7 289.1 Mean SD n 292.4 4.7 2 B 030001 B 030002 B 030003 B 030004 281.4 283.0 Mean SD n 282.2 1.1 2 C 050001 C 050002 C 050003 C 050004 276.9 267.5 Mean SD n 272.2 6.6 2 D 070001 D 070002 D 070003 D 070004 285.5 305.4 Mean SD n 295.5 14.1 2 E 090001 E 090002 E 090003 E 090004 281.1 290.4 Mean SD n 285.8 6.6 2 F 110001 F 110002 F 110003 F 110004 296.8 294.9 Mean SD n 295.9 1.3 2 Day 4 L i ver 12.147 12.155 12.151 0.000 2 11.519 11.274 11.396 0.173 2 12.534 10.301 11.417 1.578 2 12.997 12.392 12.694 0.428 2 11.495 12.497 11.996 0.708 2 13.038 12.458 12.748 0.411 2 Day 4 r. Li ver g/kg 41.08 42.04 41.56 0.68 2 40.93 39.84 40.39 0.77 2 45.26 38.51 41.89 4.78 2 45.52 40.57 43.05 3.50 2 40.89 43.03 41.96 1.51 2 43.93 42.24 43.09 1.19 2 Day 14 TermBW G 289.1 309.7 299.4 14.6 2 298.3 304.3 301.3 4.2 2 300.8 289.9 295.4 7.7 2 288.7 295.7 292.2 5.0 2 281.7 299.0 290.4 12.2 2 255.7 294.4 275.1 27.4 2 Day 14 Li ver 11.154 11.934 11.544 0.551 2 11.447 11.668 11.557 0.156 2 13.970 10.641 12.305 2.354 2 10.214 12.236 11.225 1.430 2 11.052 11.313 11.182 0.185 2 9.788 11.666 10.727 1.328 2 Day 14 r. Li ver g/kg 38.58 38.53 38.56 0.03 2 38.37 38.34 38.36 0.02 2 46.44 36.71 41.57 6.89 2 35.38 41.38 38.38 4.25 2 39.23 37.84 38.53 0.99 2 38.28 39.63 38.95 0.95 2 S t a t i s t i c s : Anova + Dunnett's te st s. (Two-Sided) * P<=5% ; * * P<=1% ; Page : 1 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 50 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats T e r m i n a 1 B o d y We i g h t & L i V e r We i g h t D a t a MA L E S 6.Test art. 6.Test art. 7.Test art. 7.Test art. 8.Test art. 8.Test art. Animal No. Day 4 TermBW G G 130001 G 130002 G 130003 G 130004 285.0 305.5 Mean SD n 295.3 14.5 2 H 150001 H 150002 H 150003 H 150004 282.6 270.1 Mean SD n 276.4 8.8 2 I 170001 I 170002 I 170003 I 170004 294.3 282.1 Mean SD n 288.2 8.6 2 Day 4 L i ver 11.965 13.261 12.613 0.916 2 11.841 10.673 11.257 0.826 2 11.339 12.552 11.946 0.858 2 S t a t i s t i c s : Anova + Dunnett's tests. Day 4 r. Li ver g/kg 41.98 43.41 42.69 1.01 2 41.90 39.52 40.71 1.69 2 38.53 44.50 41.51 4.22 2 Day 14 TermBW G 310.8 311.0 310.9 0.2 2 311.2 291.9 301.6 13.6 2 312.5 309.6 311.1 2.0 2 Day 14 Li ver 11.860 13.058 12.459 0.847 2 12.534 12.780 12.657 0.173 2 12.312 12.403 12.358 0.064 2 Day 14 r. Li ver g/kg 38.16 41.99 40.07 2.71 2 40.28 43.78 42.03 2.48 2 39.40 40.06 39.73 0.47 2 (Two-Si ded) * p<=5% ; * * p<=l% ; Page : 2 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 51 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats T e r m i n a 1 B o d y w e i g 11 t & L i v e r W e i g h t D a t a F E MA L E s Animal No. Day 4 TermGBW Control A 020003 A 020004 A 020001 A 020002 194.2 218.2 Control Mean SD n 206.2 17.0 2 l.Test art. B 040003 B 040004 B 040001 B 040002 213.0 216.1 l.Test art. Mean SD n 214.6 2.2 2 2.Test art. C 060003 C 060004 C 060001 C 060002 187.2 219.3 2.Test art. Mean SD n 203.3 22.7 2 3.Test art. D 080003 D 080004 D 080001 D 080002 206.2 228.2 3.Test art. Mean SD n 217.2 15.6 2 4.Test art. E 100003 E 100004 E 100001 E 100002 210.2 213.0 4.Test art. Mean SD n 211.6 2.0 2 5.Test art. F 120003 F 120004 F 120001 F 120002 198.3 214.4 5.Test art. Mean SD n 206.4 11.4 2 Day 4 L i ver 6.873 8.125 7.499 0.885 2 8.556 8.339 8.447 0.153 2 6.521 9.212 7.867 1.903 2 7.878 9.416 8.647 1.087 2 8.741 7.497 8.119 0.880 2 7.182 8.079 7.630 0.634 2 Day 4 r. Li ver g/kg 35.39 37.24 36.31 1.30 2 40.17 38.59 39.38 1.12 2 34.84 42.01 38.42 5.07 2 38.21 41.26 39.73 2.16 2 41.58 35.20 38.39 4.52 2 36.22 37.68 36.95 1.03 2 Day 14 TermGBW 259.9 237.1 248.5 16.1 2 242.6 231.1 236.9 8.1 2 229.3 239.5 234.4 7.2 2 237.4 244.1 240.8 4.7 2 232.1 242.5 237.3 7.4 2 248.2 226.6 237.4 15.3 2 Day 14 Li ver 9.740 9.491 9.615 0.176 2 9.870 9.950 9.910 0.056 2 10.304 10.648 10.476 0.243 2 10.911 10.452 10.681 0.325 2 9.294 9.288 9.291 0.004 2 11.125 9.102 10.114 1.430 2 Day 14 r. Li ver g/kg 37.47 40.03 38.75 1.81 2 40.68 43.05 41.87 1.67 2 44.94 44.46 44.70 0.34 2 45.96 42.82 44.39 2.22 2 40.04 38.30 39.17 1.23 2 44.82 40.17 42.50 3.29 2 S t a t i s t i c s : Anova + Dunnett's te st s. (Two-Sided) * P<=5% ; * * P<=1% ; Page : 3 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 52 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats T e r m i n a 1 B o d y We i g h t & L i V e r We i g h t D a t a F E MA L E S Animal No. Day 4 TermBW G Day 4 L i ver 6.Test art. G 140003 G 140004 G 140001 G 140002 214.5 224.4 8.305 8.422 6.Test art. Mean SD n 219.5 7.0 2 8.364 0.083 2 7.Test art. H 160003 H 160004 H 160001 H 160002 212.2 213.5 8.603 7.182 7.Test art. Mean SD n 212.9 0.9 2 7.893 1.004 2 8.Test art. I 180003 I 180004 I 180001 I 180002 199.6 225.7 6.684 7.309 8.Test art. Mean SD n 212.7 18.5 2 6.997 0.442 2 S t a t i s t i c s : Anova + Dunnett's tests. Day 4 Day 14 Day 14 Day 14 r. Li ver g/kg TermBW G Li ver r. Li ver g/kg 38.72 37.53 224.8 218.0 8.469 8.494 37.68 38.96 38.13 0.84 2 221.4 4.8 2 8.482 0.017 2 38.32 0.91 2 40.54 33.64 238.5 215.7 10.199 8.820 42.76 40.89 37.09 4.88 2 227.1 16.1 2 9.509 0.975 2 41.83 1.32 2 33.49 32.38 223.0 233.2 8.296 11.915 37.20 51.10 32.94 0.78 2 (Two-Si ded) 228.1 7.2 2 10.106 2.560 2 44.15 9.83 2 * p<=5% ; * * p<=l% ; Page : 4 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 53 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats T e r m i n a 1 B o d y we i g h t & L i v e r We i g h t D a t a MA L E S Control Control l.Test art. l.Test art. 2.Test art. 2.Test art. 3.Test art. 3.Test art. 4.Test art. 4.Test art. 5.Test art. 5.Test art. Animal No. Day 28 TermBW G A 010005 A 010006 A 010007 A 010008 324.0 336.0 Mean SD n 330.0 8.5 2 B 030005 B 030006 B 030007 B 030008 334.1 287.6 Mean SD n 310.9 32.9 2 C 050005 C 050006 C 050007 C 050008 366.0 315.6 Mean SD n 340.8 35.6 2 D 070005 D 070006 D 070007 D 070008 329.3 324.5 Mean SD n 326.9 3.4 2 E 090005 E 090006 E 090007 E 090008 335.2 324.7 Mean SD n 330.0 7.4 2 F 110005 F 110006 F 110007 F 110008 338.6 326.1 Mean SD n 332.4 8.8 2 Day 28 L i ver 12.331 12.325 12.328 0.000 2 11.979 10.361 11.170 1.144 2 13.691 11.344 12.517 1.660 2 11.821 12.279 12.050 0.324 2 11.747 12.948 12.348 0.849 2 13.507 12.174 12.840 0.942 2 Day 28 r. Li ver g/kg 38.06 36.68 37.37 0.97 2 35.85 36.03 35.94 0.12 2 37.41 35.94 36.68 1.04 2 35.90 37.84 36.87 1.37 2 35.05 39.88 37.46 3.42 2 39.89 37.33 38.61 1.81 2 Day 42 TermBW G 334.1 378.1 356.1 31.1 2 351.8 358.4 355.1 4.7 2 361.1 392.9 377.0 22.5 2 364.1 381.4 372.8 12.2 2 372.9 410.2 391.6 26.4 2 366.9 388.1 377.5 15.0 2 Day 42 Li ver 11.409 14.802 13.106 2.399 2 13.057 13.980 13.519 0.652 2 14.927 15.148 15.038 0.156 2 14.366 13.176 13.771 0.841 2 13.484 15.394 14.439 1.351 2 13.774 14.574 14.174 0.565 2 Day 42 r. Li ver g/kg 34.15 39.15 36.65 3.54 2 37.12 39.01 38.06 1.34 2 41.34 38.55 39.95 1.97 2 39.46 34.55 37.00 3.47 2 36.16 37.53 36.84 0.97 2 37.54 37.55 37.55 0.00 2 S t a t i s t i c s : Anova + Dunnett's te st s. (Two-Sided) * P<=5% ; * * P<=1% ; Page : 5 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 54 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats T e r m i n a 1 B o d y We i g h t & L i V e r We i g h t D a t a MA L E S 6.Test art. 6.Test art. 7.Test art. 7.Test art. 8.Test art. 8.Test art. Animal No. Day 28 TermBW G G 130005 G 130006 G 130007 G 130008 340.1 294.7 Mean SD n 317.4 32.1 2 H 150005 H 150006 H 150007 H 150008 319.6 331.6 Mean SD n 325.6 8.5 2 I 170005 I 170006 I 170007 I 170008 330.9 318.1 Mean SD n 324.5 9.1 2 Day 28 L i ver 12.187 11.241 11.714 0.669 2 11.292 13.481 12.387 1.547 2 12.665 11.640 12.152 0.725 2 S t a t i s t i c s : Anova + Dunnett's tests. Day 28 r. Li ver g/kg 35.83 38.14 36.99 1.63 2 35.33 40.65 37.99 3.76 2 38.27 36.59 37.43 1.19 2 Day 42 TermBW G 346.5 382.2 364.4 25.2 2 328.2 350.6 339.4 15.8 2 372.2 399.0 385.6 19.0 2 Day 42 Li ver 13.360 13.479 13.419 0.084 2 11.791 13.423 12.607 1.154 2 13.901 15.179 14.540 0.904 2 Day 42 r. Li ver g/kg 38.56 35.27 36.91 2.33 2 35.93 38.29 37.11 1.67 2 37.35 38.04 37.69 0.49 2 (Two-Si ded) * p<=5% ; * * p<=l% ; Page : 6 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 55 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r- In st. f . T o x ik o lo g ie u. Aerosol fo rsc h. Study : 01G00002 Species: Rat Sex : Both A ll sub-sets in Sprague-Dawley Rats T e r m i n a 1 B o d y we i g h t & L i v e r We i g h t D a t a F E MA L E s Animal No. Day 28 TermGBW Control A 020005 A 020006 A 020007 A 020008 232.1 258.3 Control Mean SD n 245.2 18.5 2 l.Test art. B 040005 B 040006 B 040007 B 040008 249.1 234.8 l.Test art. Mean SD n 242.0 10.1 2 2.Test art. C 060005 C 060006 C 060007 C 060008 231.7 245.6 2.Test art. Mean SD n 238.7 9.8 2 3.Test art. D 080005 D 080006 D 080007 D 080008 272.3 252.6 3.Test art. Mean SD n 262.5 13.9 2 4.Test art. E 100005 E 100006 E 100007 E 100008 237.7 219.0 4.Test art. Mean SD n 228.4 13.2 2 5.Test art. F 120005 F 120006 F 120007 F 120008 252.8 254.1 5.Test art. Mean SD n 253.5 0.9 2 Day 28 L i ver 8.001 10.907 9.454 2.055 2 9.177 8.594 8.886 0.412 2 10.087 8.372 9.230 1.212 2 11.389 9.243 10.316 1.517 2 6.943 9.557 8.250 1.848 2 10.627 11.849 11.238 0.864 2 Day 28 r. Li ver g/kg 34.47 42.23 38.35 5.48 2 36.84 36.60 36.72 0.17 2 43.53 34.09 38.81 6.68 2 41.82 36.59 39.21 3.70 2 29.21 43.64 36.42 10.20 2 42.04 46.63 44.33 3.25 2 Day 42 TermGBW 256.6 280.3 268.5 16.8 2 265.1 268.7 266.9 2.5 2 248.3 258.1 253.2 6.9 2 241.9 252.8 247.4 7.7 2 251.5 278.0 264.8 18.7 2 257.9 259.2 258.6 0.9 2 Day 42 Li ver 8.409 9.276 8.842 0.613 2 8.229 9.461 8.845 0.871 2 9.031 9.128 9.080 0.068 2 8.270 8.146 8.208 0.088 2 9.509 11.309 10.409 1.273 2 9.423 9.128 9.276 0.208 2 Day 42 r. Li ver g/kg 32.77 33.09 32.93 0.23 2 31.04 35.21 33.13 2.95 2 36.37 35.37 35.87 0.71 2 34.19 32.22 33.21 1.39 2 37.81 40.68 39.24 2.03 2 36.54 35.22 35.88 0.93 2 S t a t i s t i c s : Anova + Dunnett's te st s. (Two-Sided) * P<=5% ; * * P<=1% ; Page : 7 Exp.Unit = Animal Fraunhofer ITEM Final Report 01 G 00 0 02 [Appendices A-D] Page 56 of 56 28 Day Repeated Dermal Contact Study of 3M Test Articles in Sprague-Dawley Rats 28-Day Repeated Dermal Contact Study o f 3M Test A r t i c l e s F ra u n h o fe r-In st.. f . T o x ik o lo g ie u. Aerosol fo rsch Study : 01G00002 Species: Rat Sex : Both A l l sub - s e t s in Sprague-Dawley Rats T e r m i n a 1 B o d y We i g h t & F 1 v e r We i g h t D a t a F E MA F E S Animal No. Day 28 TermBW G Day 28 Fi ver 6.Test art. G 140005 G 140006 G 140007 G 140008 237.3 248.6 8.024 8.110 6.Test art. Mean SD n 243.0 8.0 2 8.067 0.061 2 7.Test art. H 160005 H 160006 H 160007 H 160008 251.6 244.6 10.795 8.330 7.Test art. Mean SD n 248.1 4.9 2 9.563 1.742 2 8.Test art. I 180005 I 180006 I 180007 I 180008 237.5 250.2 9.872 10.574 8.Test art. Mean SD n 243.9 9.0 2 10.223 0.496 2 S t a t i s t i c s : Anova + Dunnett's t e s t s . Day 28 Day 42 Day 42 Day 42 r. Fi ver g/kg TermBW G Fi ver r. Fi ver g/kg 33.81 32.62 245.4 260.8 8.967 9.491 36.54 36.39 33.22 0.84 2 253.1 10.9 2 9.229 0.370 2 36.47 0.10 2 42.90 34.06 219.9 255.9 6.636 9.001 30.18 35.17 38.48 6.26 2 237.9 25.5 2 7.819 1.672 2 32.68 3.53 2 41.57 42.26 257.2 295.7 8.521 11.082 33.13 37.48 41.91 0.49 2 (Two-Si ded) 276.5 27.2 2 9.802 1.811 2 35.30 3.07 2 * P<=5% ; ** P<=1^: ; END OF REPORT * * * Page : 8 Exp.Unit = Animal