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E. I. du Pont de Nem-urs and Co., Inc. Haskell laboratory for Toxicology and Industrial Medicine Elkton Road, P. 0. Box 50, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 560-81 Material Tested 1) Octanolc acid, pentadeca- fluoro-, ammonium salt* Haskell NoT 1) 12,037 Other Codes C-8 2) 14,008 Study Initiated/Completed 6/2/81 - 6/18-81 _ Material Submitted by CR&D Department^ Haskell Laboratory MOUSE FEEDING STUDY - 14 DAY Procedure : Eighty male and 80 female albino Crl:CD-l mice (age 41 and 42 days, weighing 19 to 30 g) were separated by body weight into 5 groups according to computerized randomization. The mice were housed individually and fed either C-8 (H ^lz.037) #14,008) mixed with Purina Lab Chow for 14 consecutive days. Concentrations tested were 10, 30, 100, 300, 1,000, 3,000, and 10,000 ppm (for each material). Separate male and female controls were observed concurrently and fed only ground Purina Lab Chow. Individual body weights were recorded 3 times/week, food consumption weekly., clinical signs daily, and liver weights at the 14-day sacrifice. 1- - Company Sanitized. Does not contain T S C A CB i Results Female Nice C-8 t (H-12,037) t | Concentration ppm Average Body Wts. after feeding (g) 1 Week 2 Weeks Average Liver Weights (g) Average Liver Wt/ Body Wt Ratio (g/100 g body wt) 0 10 30 100 300 1000 3000 10.00C 23.8 23.9 23.5 24.1 21.6 16.2 13.8 A.D.** 24.9 26.0 24.7 22.8 18.2 14.8 A.D. A.D. 1.53 3.02 3.24 3.94 3.39 2.27 ' -' - 6.14 11.62 13.11 17.28 18.63 15.34 -- -- Concentration ppm 0 10 30 ion 300 1000 3000 in,noo Male Mice C-R (H-12,037) Concentration ppm 0 10 30 100 300 1000 3000 10,000 Average Body Wts. after feeding (g) 1 Week 2 Weeks 23.5 23.4 23.7 23.P 23.5 23. 3 21.7 1P .P 25.1 24.5 25.3 24.7 21.9 25.1 24.1 20.0 Average Liver Weights (g) 1.55 1.83 1.992.09 2.94 3.26 3.14 1. 56 Average Liver Wt/ Body Wt Ratio (g/100 g body wt] 6.18 7.47 7.87 8.46 13.42 12.99 13.03 12.80 Average Body Wts. after feeding (g) 1 Week 2 Weeks Average Liver Weights (p) 27.1 28.9 28.1 26.4 25.8 19.1 A.D. 18.7 28.3 30.8 , 27.9 23.7 23.1 17.4 A.D. A.D. 1.69 4.13 4.40 4.48 4.10 2.48 - - Average Liver Wt/ Body Wt Ratio (g/100 g body wt) 5.97 13.41 15.77 18.90 17.75 14.25 _ - 2Company Sanitized, Does not contain T3CA CBi I mm Results (Cont'd.) (H-14.008) Concentration ppm Average Body Wts. after feeding (g) 1 Week 2 Weeks Average Liver Weights (g) Average Liver Wt/ Body Wt Ratio (g/100 g body wt) 0 10 30 100 300 1000 3000 10,000 28.9 27.7 28.5 27.2 28.2 27.1 25.2 22.7 29.7 29.3 28.7 27.5 30.3 28.8 27.4 23.f 1.77 2.75 2.36 2.38 3.41 3.03 3.25 2.87 5.96 9.39 8.22 8.65 11.25 10.52 11.86 12.16 Clinical Observations: Concentration PPm C-8 Weight loss. Observations Weight loss. IP Stained perineal area, Weight loss. weight loss. 30 Weight loss. Weight loss. ion Weakness, piloerection, Weight loss, stained perineal area, weight loss. 300 Unkenpt head area, Weight loss, weakness, tremors, wet and stained ierineal area. 1000 3000 Death, unkempt head area. weakness, tremors, wet and stained perineal area, piloerection, weight loss. Death, unkempt head ar,,-a, weakness, piloerection, weight loss. . Weight loss, Unkempt head area, weight loss. 10,000 Death, unkempt head area, pallor, weight loss. Unkempt head area, weakness, weight loss. - 3- Sanitized. Does not contain T S C A C B i Company _ r _ a nri fed to Bale and female^Crl:CD-l mice 10 p^ to l0,? ppn) for 14 consecutive days. Death occurred at l . O M p p a ^ 3,000 Ppm and 10,000 pic in the C-8 mice. No deaths occurred in the nice. Body^weight loss was indicated at the end of each week at 300 ppm, 1,000 ppm, 3,000 ppm or 10,000 ppm in C-8 male and female mice. showed a slightly lower rate of body weight gain at 10,000 ppm in male and female mice. Liver weight/body weight ratios showed a sharp Increase at 10 ppm in m a l ^ n ^ e m a l ^ ^ ^ ^ ^ v e ^ h e controls and showed a dose-response increase. J mice showed major increases in liver weight/body we:lght rat^ aV * ^ 1.000 ppm, 3,000 ppm and 10,000 ppm in males and females. M a l y n ^ e m a l e r-fl mice had heavier livers at lower concentrations than c-8 c" "1" 1 *Ins 1"cluded; "eatoesf' tre"r 8 > piloerection, palUr, stained perineal a r e a ^ e i g h ^ o e ^ n ^ i n l j e M . appearances from 100 ppm to 10,000 ppm. clinical signs included: unkempt appearance weakness and weight loss at 3.000 ppm and 10,000 ppm. In male and female mice C-8 produced greater liver weiRil* hangaS reduced body weights a t l o w e r concentrations (from 10 ppm to 300 ppm) than At 1,000, 3,000 and 10,000 ppm C-8 produced not. Extensive clinical signs were . S e K e ^ ^ S I ^ i W S ^ l ^ r . . whereas , exhibited. 100 p m to :10.000 ppm ef C-8, overt abnormal! t-ies were /g - 4- . .PotoortainTSCACS! Sanitizsc. fepUi.*-' mn'i aii am ** A.D. - All died 0. Louis Dashiell Study Director Approved by: LSF:jrg:WP:i 3.4 _ Date Issued: October 26, 1981 " Report No. 560-81 J Gerald L. Kennedy, tr. Section Supervisor Acute Investigations - 5ompany Sanitized. Does no., ccoonnttain TS C A CBI .. ._ t i . - .......... *J.p