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TOXICITY TO AQUATIC PLANTS AR 226 -- 0727 | Identity: A mixture containing perfluorooctanesulfonate, which may also be OrecftearnreesdultofoansicPFacOiSd,) (FCC-A9S5#, 2or79a5s-3a9-c3o)m.ponent of FC-203. 1- iRseFmCa-r2k0s3:. diethylene gClyuTcrohrleenbt3utMiynlfpeortrohmdeaurtc,ito3ino7n.in8ldo5ti%cnauwtaemtsbeeirt,ris2wa0as%minxeotttuhryneloetoendef.gl1yT.c3ho4el%, PteFsOtsSa,m3pl5e% 2.66 % Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrationsofimpurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. M0 emo0 n.00000000000 Method: OECD Guideline 201 Type: Acute Static YGeLaPr:stNuody performed: 1991 SSpoeucricees:: ISne-lheonusaestcrulutmurceas.priOcriogmiunatlulmy obtained from the Culture Collection of Algae at the University of Texas at Austin. Element basis: Algal cell counts (cellsiml), growth rates. Exposure period: 96 hours pStraotgirstaimcaolf MSettephhoadns,: 19T8h3e. ETCh5e0NvOalEuCeswwaesrecaclacluclualtaetdedusuisnigngDutnhneetcto'msptuetste,r Analytical monitoring: pH and temperature. Test Conditions: Algal Nutrient Medium: Nutrient medium per U.S. EPA, 1978. This nutrient medium provided all mineral nutrients essential for algal growth and also mseerdvieudmawsatshe~d8i.l0u.ent for all algal operations. The pH of this syntheticalgal Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared in nutrient medium. Aliquots were then added directly to the test vessels to create test solutions. Exposure vessels: 250 ml Erlenmeyer containing 100 ml test solution and capped with inverted glass beakers. Agitation: Shaken continuously at 100 rpm. 000639 Number of replicates: 3 INnuitmiablecrelolflcooandicnegn:tra1t.0ioxns1:0 fcievlelsp/lmuLs. a blank control WLiagthetrinCgh:em3i5sturEyi:n/sec/m? from continuous cool-white fluorescent lighting. pH range (0-96 hours): 7.89.4 (control exposure) 7.8 -8.0 (1000 mg/L exposure) Test temperature range (0-96 hours): 23.3 - 23.4 C (incubator) Resuwys 000000000 OOO00 Nominal concentrations: Blank control, 62.5, 125, 250, 500, and 1000 mg/L 96-Hour Algal Growth Response Values Nominal | Mean Cell-Count Conc. | (cells/mL) (mall) 395.000 645,000 347,000 [250 69,000 [500] 51,000 [1000] 12,000 Average | Percent Change in Specific Growth Rate from Growth Rate | Control (96 hrs) 00% | -- | [o 1o16d| 0.037 0.020 0.017 0.002 Algal Growth Response E/Cs values: 9966--hhoouurr EN,OCsEpC==355400(3m2g3/L-- 389) mg/L Element values are based on nominal concentrations. Reversibility of Growth Inhibition: Not give. } Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. conclusions 0000 3T8h9e)tmegs/tLs.ubTshtaen9c6e-ehxohuirbiNtsoaOb9s6e-rhovuerdEE/fCfseoctgrCoonwctehntrartaetivoanlu(eNoOfE3C5)4 i(s352030-- mglL for growth rate. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 000650 p0 araguaury 000 tReesltiianbgi.litHyo:weKvieirm,isschamrpalnekipnugri=ty2.waThsisnosttupdroypmeerleytschtahreacctreitreirzieadfoarnqduatlhietystudy lacks analytical solution. confirmation of the amount of fluorochemical proportion in the R0 EFERENCES 00 o"TrhiHsatmepsttowna,sNcHonadtutchteedrebqyueEsntvoifrotSheys3tMemCsoDmipvaisniyon,, LRaebsoRuerqcueesAtnanlyusmtbse,rInc., H2864-9, 1991. orWeR~ 00000000000-0000 Last changed: 6/27/00 000641 "- BEST COPY AVAILABLE stots Title to TshteatRicnacutSeerTeoxmisciirtys oCfopFrCic2o0m3aten { rToaAtuithborsn || | 5 une, 1991 oA CChE atRhI aERmS L Petoraing Laboratory i n Sh atGaniatearEaitntneicRosad Sfaoid ol S5ed STIR T Tae 4 LE Solis Sgt hge gE FRET SERRE LE RISO Tash Gale Ga Sl RainCAR B gi et ns 14wo phE AMBRE;e IREEE EC ClCoE mE inERMIE eaMR a ? 000642 . "BEST COPY AVAILABLE 1. sven fECcooorrnvRrieuirCcoetoetPrestemeepgnuTnsosrstDTeiW3vsicsitoo"omSnTxaeisnccyiratthtyiTeotRreosf"tor1a3uc6rhcTFoIehCuirssRM1osa3tnietN n"sgt,oPcoeTttahriess,osiO1g10s0L ,00%,essekltSel5esn"skesestttr1rh0eoe2n SEITrors tobert moert, ad Facts rene cCoanmceernsti rtTautecieons"ottetcof"i32.mT6de5as6,t Hone an Sasieied ta vepbee"erTnvfoet.omrSemeish.teiasotwossabtsevsree.BeerrstsieaeatmseiecTovecipopernnetiliitseitoi2noosontsiswiithhFrfetipfavitevede 35 abiarseer oa ene eT hake Lfeoimrtetrolelg , R$2.3"gwarm,S listMarih e 250 wh er ear os oo CTnoiOnnIttaaLtiEtnBaearedi1eoan""unavtee"srmpToetEmce"tsithvoec"uatorfyn:d2iBhteikomo-- naso.fr 0EF8iopewoesr0seTheoonfeosaRleo-- seseeaneentoeohkebreeeesSpEeAEeL . 4 Sak i wh sony is RE de ollFr EE souE pmnt Sanit fhianbeer : Ripa hem 5 er BnAE SpE TL ess i E SE niE si es eR a Ba ER ME SRN SaEX eo : A FE Sl ReC Tarae nelei 5 dado . FL Ea SERhA ts OnStEE RRhYa A SSfrBeid nde vise PRI i AR 18 ss PR IGRI gd Sen Ga BEat TRAE el TCRRIEpE eE aE k 5 IY SSER n mea Sl Ss a LEE Ee eat ann i ReA r aE Aha a HEsLeiaab dH NL oa. 000643 e me e-------------------- * BEST COPY AVAILABLE 1.Tan or comms . scrton: ne metots sot eteriats ; Toendix A. TaterQuality ate from Toxicity Test 2 % = c er >5 i Fshieiilonoatln BABER R I aG Te. got dire es UR SSEi e (Oe E E Cema=i I rata S S r EE Ree E A E SRR raR n EY = Co So Ge RE aS l CRRA GRTE E Me e 000644 --_-- BEST COPY AVAILABLE Er------ Tole 1. ea ontieclarcRhareaE cttearisartaionn ooff3 rEonmeesemtaative ws 6 TO 3. FS urcat C change froncoT ntol l 1ndsverige Tle t. Npebian eiftasctinae comeatrations (508 n Tile 3.1.Tenperatuzesesiurd duieg toxicity tet 14 Tle 0.2. 5 snared ducagtoric text s fit % RST Th elt S Bo t hon ARi R t ea BERe as Sedna HEE a Set Eps Ca TRL Gi 2Sawl eg: CampiaL al Sais lee a r va RR EA GEA Se Sh : oY nh FE be fi STE Caleta a S LE ESSa Rse SiR e Sm ESiI s ye . 000645 BEST COPY AVAILABLE _---- mv. oemooverion r aCbrieescituiavtTE eehrisof`"vsltguta.dhsecsavptaTtushodepyesnvrpaeospnosrTvotnoreScdoentbttheyeari3an%isCeaosemtrhcpotainryoyno,stasStth.ettoPeradeuetlbo,crhrWiainmseseeRbnseEoeAtRaooS:iathoTdh0se Thoeveasttiphatiqouna.litTyoedatreepocrotl.levciiseedcoSnutraiienns.tthen SLpopoee"ssia thot presents LEa -- Test smsmace: . BEEao0vtit0cloesyFaotth"eaRntes,a.2003)vaosna(GRcMaeluvvebieaecrsloilseesyrdasbteewrs1is3,iThhSua1i3m`st3p)h0le,evfaoTNsl+ulasobueweepirspnbgiceoa2dnt6to9oar9iye)nseotdhiwoic1nsao:m+edmessnhlteiSvemeeErobenadiutsTteoe hVArEeeTndsBS,iarkS"r55c.iheiPvaTeuodlo:kattMieEnmonpveeisrroaottsauy:reet.ePnrsAiofrroersfooeramvsieentieotmnhpsnleto.ets(t1opBeeaetseinriimttenvesr1s1eamreedat]n sruoion rE: SStoEepEsrptoiixenigaSaaottvteaarnldyeiwSoi"ien3dr0li.ileed.forsBwoeaatsvcimeccirlhtieemnwaetoeifmdoendefsohofempit[eeeOeksiplkt.eeors.gpEaeoniihiyesseisssIeOtaotu)fsd oftorrehaplralesteerxCtihtciiieot}sy bee hresematisy Tne g mows loo E5E3% Et2u5h7le7siceruelAatl'guartee.oE-uaCsvoeitdvloefcsoerriaottnah'aessoftoensAtgSweoaersiatf1r4ot,mh'1eaoT3ac0iurvletruhsrieetosrtaiefgeinTntoairlnslyhyoofpreotpchauesremeidn'fsrsoomd SSho G{aEEEhT h fFeL ntsrfraernitsiL esdftoreu:asrlteargeiladenA }FeuoprnopR ecladtinec;dciE SaarxhoindceaenitcidcenaSlettyoo'.seTdies" vcauldtufroer Htwheeisn LS Sg SYR Eu Geni x Em me SE eT AEE 0 in iin Ji a ners SAEAIRMEI D 1c9o9n1du5cteTd Jhata<a tbaarsgeedt tSeomnpeprraotcuerdeicesFofi32 orath29eC,'wiOtEhCDfi(v1e980c)o.nceTnhterattieosntsvaosf 7 EWE test toubstance fand'a dilution water control SA 1,000 mg/L stock "oFfEEFY wa aTsutipornepawraetderincVodnitlauitnieodn solution w In a 5 tht eThtee essttor icvkesssoellustiownitwhaosutaddseadedirmeecctlyLettoo dEG 30rEienae5dsolves nt. Lsa NonStno ealbr caoncienetrae tieons oot ethe tesFtT m0 aterial `were: 0ng:/Z L N lonteT eE saSM I T LST esd Lono A TUEBE e SC Pa Se ERRA [i 000646 -_--m---- ' BEST COPY AVAILABLE Tel. efmicialechraracsterisatiRon Iof 8Ryrempreseentatlive esampaleReof Ps r ----e-- maseemen-- t h-- Tetm---- eemater esm r fottit-- en wn : - [ pe rr -- os " rToiltgeebrtosriaated aa os " Fore To = not detected oF or shore he report TE Biter SEE Vn pl Seem wSog Sa eAt R ens TTiEnieE el sE aba SOHNE diag: Lh aE B FizNeReA a ny a Ea o ShaShOn T ARa E SLgE DRO Tame SEES CL ERIC EET Mame . aa ACIR E T G He e ee T Se aalal s: Vida vam got 38jn SIE Nea EER og a SRR BESca ve.siEEiR AL C SEEh R S er na AMTERHN ST S AE eea 000647 A : BEST COPY AVAILABLE 0wreiplitcDeaesttrefso.rssooelfdutiIo"inen'a.ch25`LT0rheeaaltfa1eginlstaksssatwBerrtNleheeEncosFpeirrteeedrofFvilti1hc0-- k,s0I0o5vtehecrtetteinEoo/pmathtsorinnehsvee0ip0borroeskt TdTeuosrctia!ntgiToensthteio.sf.96"evahecorhusrverstaesnseatlo.)a.l(y1 Thararn3a4dnobgnoeadmroosnb1eiagrhst.rtoA2tB3aL8re.y0svhehosaukraereSdaforktoapnhsoefhtoeoenpntebeiitooorsr wfiaskeaaiatlyoeboifne3d5 vwiEths:rcrosn:d ubite fluorescent Lights thet proribns orm Reveiicmshiicyt"tstToehaneteetveeo"rEurisysbsekr3T4h"SeoE"fhtoDahurElesg.ba(elBgbeiyctaeknlmailseansss/essaalatonefdaledniPedrAaYeccohtf12ttmehiesetctreotvrsee)rcstos,pesliscowbSsaacsvtseederressstpiernrEreaeidinnaneiSdnn fOaScTtorer:cury Thermometer)" was measured `and recorded dai ia the sanzstion, wEraons: CSsoetntachteoniltsrRtavetiasicsuolnts.utsescehdooffaiqByuLt.eehssetthet(soSuartubletapchthioaatrnny",cet.ot1e98sc9ta)l.TihvueTei.hraeetBeObRBiiEnEaStc)evraivpiarsleetocercedamlsooevbviiyienesybsetmsnaornaeodsicaiearynet ke: . : TDTauhcneinieot`ctae'i'st.ura(dNteOslTto,g. owfhiTch"htehe.aivnmecerlaaubgdeeersspofeacciepfluilrcsamoperteertiahc1rseaetteeO-Fsveeosyvcesorptrosiriptmeaieteeedthooerf naturel dog of the umber of cellspera1at 0hours divibdyethde % iid eaSvxiepbrotaseguaercetisap`peecritifhoiedc. specific `Groeth `sgurTophweet'h Aprevaretcreea,ngte dsicphvaeincdgiiefn.igcfrtghroaonte.tchonvtesrlaostlee 1fbsryo.cn rttlhheeeicsovamettreieoodykd ritedatheconntdaurltioplsliag that ssibyesosn: pig Teenpeercent chanPge ETroRe Econtsh vas wetheSoekute SERSaah gk se REE TE dm Brita Ry Ee TLoellnemar S en Ca oe lf nimalisgeF l iE C BR ite gaae ta sidda uPgEL TRRE RT TY. Pn RE ES SeOEREE: sgSESSLP UIERR PYRCL 4 : . 27 CalReeEr .TLaleassdaSRn SSEOEIGE Se JEL, ATSOie ia S SIERS ra eTEayS CeR GR a i i SR cn ners = . DR ee rm amar Lie,Se. 000648 e-- e ---------------------------- BEST COPY AVAILABLE VI. RESULTS ' t1B3o4xriceiCtUyMBnipotelSroecgneftnetalanorfee1c`3h!Bdartorsawelabttenersd TaqovueaFrlaiagtteyesdp3aeatcmaifigScoepsveeemroaavitteetndFibPteyL,Etbhe8ee,csriatceiuntsae PRRoEvoe G oPiRTGsRee R 31Ss.on3s340abwC r,ork72T.C a30nl3d.e98ATdeour36i5h5o0utsr BsfEoSrOaSilbeoA n0e4expeoossea dootkoFtChe30132 : nERCEees AP ges n E ORE CG Ho OEaBy Ae we iExz PAE eG Te R a alls RETR Re Ll ei a BAIRl IoEdieSR SL e on Ee Le HAE es : sSTraees E of 1 DE RER N big + i smNLh mares CH Sobendiiii EL BT 000649 per re------------------------------ BEST COPY AVAILABLE Table 2. Growth data from toxicity test : --- w c--oxn_ocwmeinnthartation ee. TNFuUsoeTrOwnoffmCceem llllss ppeerrm MNDilMlim ttieterr2x10100 G(* howouwr)) 0C.o0ren EP 35 A .o 3 mnB o NosAaw am mm o om m as wT F 5 e % om T 8w wo wnA a oe m moM m 1s PiSoooonnn we 1 xox xonom om 5 aw u m n ba 5 #. 0 5iooonnoon wo h kow ogaow 28 aaa " oau 3 : a 0 500 tibia 1S Poo o1d0nd.oon -.ow32 a3o2R 3in0 demon20 0038 a538 Hl ABiieaR n s alG Lovo: 1T onn mao lcooonk La x% Eo R aontm 3 ESRER7 Bray o owmeS n eaa nHd TEa1l0ie e4an vcE2me)se 38 3s 2 nTeFi ee1t2 . Ee RNb ETOE ae EER0E S3E cha eEyT F EoElEge CSCEE STR ea SRR rSE eas Ee BE SeintTE SeEaRS AsEEia mrCeSERSEEea K e Gidi ai lel e aAi L IP E RR LESR Ra ca OTE a CI e DiTR 000650 BEST COPY AVAILABLE ible 3. Ppescpcentt ochaengegfhron control and average specific growth rate Toe fcToonsct tentSurbasttiaonnces C memo e e IrMatn ee mma trroemcw oonsttreoarln eoe ra Tor e a Te m ewendtar ee2Tmre30ve: 0.0 (wntrol) 0.005 0.04 0.08 0.0% 0 oo 6s 0.062 0.0 0.07 000 m1 1s oom oom oom 00m 2 1 am 0 0.0% 00M oom oom 1 Bu a so 00s oom oo oor a6 me ss 1000 0.0% oom oon 00 mae ---------------------------------------- SS e R SE ene, STraina d e C rt E SDeYgRIGARNS b e e a BRE Ttoe IRCTReEE OR i ce ERT Fa a lan TRE Te ZEomEERIR TLE R | a Sa E ae C i Re aa BE Rh SESE EEE Gr a TUE CESS Re rT BfE5ra4on RRAEREL A S Te E iE g Li"l1i=EalleRRAa 000651 | Tablet. Natisn effective concentrations (BES0R) from toxicity test --rTre-- ryee----s--s ----c-- SontTeitE --ipaacreeT-- nTtaEttE--s --C--otre --os e--e PT p---- -- -- hows 1,000 man -- -- Ths Msn B3-sSwn series averse Shows dsesn mo wi sorisg were -------------------------------------------- 2 o Lesa ihaadlie a L e e E Se E ERLA s ESHdEN we lSeoen o ERSHUaTR Sie RATE ORE ElofT e SS Re ST Ea PEdRd RIa S nL Se Elneey hy Cfoa TPt R BPee, rem EASE meng Sa RE MEi R AEL E a RE OR BINGa e Elemmnaan RRT,ER L LC MRoen EVRETT TTm it WEES SE LaL es 000652 I: E BEST COPY AVAILABLE] | | wi. wrmmas i SSeIrDie s1e9oe0e4e.Gude"bRioReRs fSo ToestiTng oLf hetTceEls.SsecEtion 3:ttete on S4S,i1bU9i84,. SCrha1.iCommer pros for culotaion of 10 viees, 1|' US.oSt.ne EFPoAi.. BatoSEnA 1978. The Selenastrus SEcapricornutus Printz Algal Assay i ono Alle 6 | os EL : | ER Ll UE RE rs SRE { ge a Re BRE Lg SET LFeEnlD ae CI aR Ee RRSify Ae RH Se ne maa Tesddimeglian SR | Ea Cri FhEa Ec Mn E Fa Ign LL ALEE Saia EN z 4 SRS a # # Ry : |4 R T Brlerl mnLaha ERH ED n Cilmi LCSmeE sognen iSliresmiiR t 8D a bs TT TERR 000653 Ls -- BEST COPY AVAILABLE AppendixA. VATERQUALITY DATAYRoNTorIcTTy vEst S 1 a TE TE An ae amie T peesa : n3l ra Sel ayy fo TERE SERVE ARE Ten CURRRT S 3) g id BE TR ie Fig ThmRE Gy iBda Lege EM NE odE ne T TER a IBoE RARE A al Be REL URS Bp bl a i ekSe i i et 000654 BEST COPY AVAILABLE . ible f.1. Tenpersture sessed tuios tostetny test Twasorfy Bredeetar o ms ) Cas 2 a s 4 ` 4 -- ee ------ ae i A Ahheass : H HL E aS tSEeRE TRAFNRETEL rd EGE35H4 SS L BR RR LE a REAP aig L TRT EEE S a LaE ee tac 3 PSE FWAERYRRREONE ToBamael ann rA e S se bh FREER ; EE -- ern. 000655 BEST COPY AVAILABLE Table 0.2. BR ensured duriog toxicity test . T-- coSnT cioeomrtn-- iation ----rest-- icate ------ E_a_ -- = -- ie erin tA |Frei 0 contrat) 3i3 SopnTrs 5]5.0 as 1i3 aBT Y a55.5 1s 333 7i1 3I3 0 i:3 BbnmyY Bv3sH 500 3F3R I=bnY vIBs]H 1.000 : 323 ] TIIin vst0oo Hoda TE t Jam SRE i Eo Fa rN ERR Eh et LATER Sl Bet LoHoldi hi ETAEONE EMReReR SAe d eiaiiie % FETA - edt > EI CR feist :3 sl aei TR m SEe iNn aedHERAT SE E aE n eae aeie Sh PRT : 7NRE GEEASRAG Rrseb bai EC ---- 000656