Document oeJjqx2JYJM2rbQROD9LQZXNo

CRI NUMBER /to * l DOW IDENTIAL INFORMATION % R & D REPORT DOW CHEMICAL U.S.A. RAO REPORTS SHOULD REMAIN ON THE PREMISES OF THE DOW CHEMICAL COMPANY APARTMENT Health and Environmental Sciences, USA TiTLE VINYL CHLORIDE: RESULTS OF TWO SHORT-TERM INHALATION STUDIES lri number LABORATORY REPORT CODE HET-K-1711-C29) OATS IIJUCO February 3, 1981 LA tl. NO. NO. 9,1.7 0,0,0,0,2,7,3 43 PACES IN FULL REPORT K. A. Johnson, H. 0. Yakel `fca^C2^"~ $tSi4U 77 Fla 3i / i signature M. J. McKenna A>h,c^~- /n DESCRIPTIVE SUMMAA R17Yy'/ WITH CONCLUSIONS: This report is: | | INTERIM [xj FINAL and main NEW REVIEW A thirty-day cooperative study designed to examine the pulmonary effects of vinyl chloride (VC) inhalation exposure in male CD-I mice. Golden Syrian hamsters, and Sprague-Dawley rats was terminated after only 4 exposures due to unexpected early mortality in male mice. Animals were exposed to 0, 1000, or 5000 ppm VC (mice and hamsters only), 6 hours/day. By the fourth exposure, 4 of 30 mice exposed to 1000 ppm were dead and 2 more were moribund. There was no mortality in either of the other groups or species. VC-induced hepatic necrosis was determined to be the cause of death based upon viral serology, gross, and histopathologic examination. A second study utilizing only five male CD-I mice at each of the same exposure levels for 5 exposures produced similar results. Clinically, several mice exposed to either 1000 or 5000 ppm VC were lethargic and had distended abdomens. Gross necropsy exami nation disclosed ascites, a variable degree of subcutaneous edema, and liver lesions. The livers were generally swollen, congested, and had variable sized necrotic areas. Histopathological examination disclosed centrilobular to massive hepatic necrosis. Lesions considered secondary to the hepatic necrosis were noted involving the lymphoid and circulatory systems. Mice surviving the exposures and allowed to recover for up to 13 days had evidence of hepatic reparative and regenerative processes. Generally, the hepatic lesions were more severe in mice exposed to 1000 ppm than in mice exposed to 5000 ppm VC. However, there was wide variability within each exposure group. In contrast to mice, there were no significant lesions noted in either hamsters or rats. The results of these two studies should be considered when evaluating results from experiments with vinyl chloride utilizing mice. DISTRIBUTION-. DEPARTMENT files RID ADMINISTRATION CENTRAL REPORT INDEX ( 566 Bldg* - Midland) -4 COPIES FORM C 4300 PRINTED R 3 e0 Distribution list is continued on attached page. DO 137960 CONFTDFNTTAI VINYL CHLORIDE: RESULTS OF TWO SHORT-TERM INHALATION STUDIES K. A. Johnson and H. 0. Yakel Reviewed by: M. J. McKenna Final Report February 3, 1981 Toxicology Research Laboratory Health and Environmental Sciences, USA Dow Chemical U.S.A. Midland, Michigan 48640 DO 137961 CONF TDNTTAi oo oo to o\ O' TABLE OF CONTENTS SUMMARY ........................................................................................................... 1 INTRODUCTION.................................................................................................. 2 MATERIALS AND METHODS ..................................................... General Study Design............................................. Test Material .......................................................... Animals ...................................................................... Chambers, Vapor Generation, and Analysis. , Exposure Regimen..................................................... Microbiological Determinations......................... Necropsy and Gross Pathology............................. Histological Preparations ................................. RESULTS . .......................................................................... Chamber Analysis...................................................... Animal Observations ............................................. Microbiological Determinations......................... Gross Pathology ..................................................... Histopathology....................................................................................... 11 DISCUSSION...................................................................................................... ...... SIGNATURE PAGE....................................................................................................23 QUALITY ASSURANCE STATEMENT................................................. .... . . , 24 REFERENCES............................................................................................................23 TABLES 1-10................................................................................................... 27 DO 137962 CONFTDFNTTAt SUMMARY A thirty-day cooperative study designed to examine the pulmonary effects of vinyl chloride (VC) inhalation exposure in male CD-I mice. Golden Syrian hamsters, and Sprague-Dawley rats was terminated after only A exposures due to unexpected early mortality in male mice. Animals were exposed to 0, 1000, or 5000 ppm VC (mice and hamsters only), 6 hours/day. By the fourth exposure, 4 of 30 mice exposed to 1000 ppm were dead and 2 more were moribund. There was no mortality in either of the other groups or species. VC-induced hepatic necrosis was determined to be the cause of death based upon viral serology, gross, and histopathologic examination. A second study utilizing only five male CD-I mice at each of the same exposure levels for 5 exposures produced similar results. Clinically, several mice exposed to either 1000 or 5000 ppm VC were lethargic and had distended abdomens. Gross necropsy examination disclosed ascites, a variable degree of subcutaneous edema, and liver lesions. The livers were generally swollen, congested, and had variable sized necrotic areas. Histopathological examination disclosed centrilobular to massive hepatic necrosis. Lesions considered secondary to the hepatic necrosis were noted involving the lymphoid and circulatory systems. Mice sur viving the exposures and allowed to recover for up to 13 days had evidence of hepatic reparative and regenerative processes. Generally, the hepatic lesions were more severe in mice exposed to 1000 ppm than in mice exposed to 5000 ppm VC. However, there was wide vari ability within each exposure group. In contrast to mice, there were no significant lesions noted in either hamsters or rats. The results of these two studies should be considered when evaluating results from experiments with vinyl chloride utilizing mice. 00 137963 CONFIDENTIAL -2- IINTRODUCTION As part of a cooperative study on the pulmonary effects of Inhaled vinyl chloride (VC), male mice and hamsters were exposed to concentrations of 0, 1000, or 5000 ppm VC by the Inhalation Toxicology Section of the Toxicology Research Laboratory. To make efficient use of the Inhalation chambers, male rats from a study on molecular mechanisms of carcino genesis were Included In the 0 and 1000 ppm exposure groups. The exposures were originally scheduled to last for 30 days but early mortality and other adverse effects were noted, necessitating the premature termination of the study after 4 exposures. Because preliminary Investigations Indicated that the mortality was possibly due to unexpected acute hepatotoxic effects of VC at these exposure levels, a second small probe study, utilizing only male mice at each of the above exposure levels, was performed. This second study was designed to test the reproducibility of the previous observations and to obtain better necropsy specimens for histopathological characterization of acute lesions induced by VC Inhalation. This report will present the results of these two studies. Emphasis will be placed upon the histopathological description of the lesions. 00 137964 confidential MATERIALS AND METHODS General Study Design The Initial study reported herein was a continuation of collaborative research pursued jointly with Dr. Yasunosuki Suzuki, Research Professor of Community Medicine, Mt. Sinai School of Medicine, New York, New York under a grant he received from the NIH. The grant was for Investigation into the pulmonary effects of vinyl chloride exposure in mice and hamsters. This was a cooperative effort wherein the exposures to VC were to be conducted at the Dow Toxicology Research Laboratory and the animals were to be shipped alive to Dr. Suzuki at the completion of the exposure period for specimen collection, processing and evaluation. This study contained 30 male mice and 10 male hamsters at exposure levels of 0, 1000, or 5000 ppm VC. In addition, 10 male rats were included in the 0 and 1000 ppm exposure groups as part of a separate study on molecular mechanisms of carcinogenesis (Stott, 1979). For purposes of this report, this cooperative study will be termed Study A. This study was scheduled to last for 30 days but was terminated after 4 exposures. The second study, hereafter termed Study B, consisted of 5 male mice at each of the three above exposure levels. This study was scheduled, and lasted, for 5 consecutive daily exposures. Test Material The vinyl chloride was supplied by The Dow Chemical Company and assayed for purity by gas chromatography by The Dow Chemical Company Analytical Laboratories, Midland, Michigan. The sample was found to be 99.9% pure (Cortes, 1979). Traces of methyl*chloride and vinylidene chloride were found to be the only impurities. DO 137965 confidential -4- Animals The mice used in both Study A and Study B were male CD-I mice, 5-6 weeks old (Charles River Breeding Laboratories, Inc., Portage, Michigan). Hamsters were 4-6 week old male Golden Syrian hamsters (Engle Laborat ry Animals, Inc., Farmersburg, Indiana). The rats were male Sprague-Dawley rats weighing about 225 grams (Spartan Laboratories, Haslett, Michigan). The animals were acclimated to the laboratory for at least 11 days prior to the initial exposure to VC. The animals were randomized from a single lot for each species (separate lots for mice in Study A and Study B) by use of a computer generated randomization procedure. The mice and rats were individually identified by a numbered metal ear tag. The hamsters were individually identified by cage and rack numbers. The animals were housed (and exposed) 1 animal/cage in stainless steel mesh cages. The animals were housed in rooms designed to maintain a controlled environment of humidity (40 to 602), temperature (22i2C), a 12 hour photocycle, and 12 air changes/hour. Food (Ralston Purina rodent chow) and water were available ad libitum except during exposure. The animals in Study A were all weighed the day prior to the initial exposure to VC but subsequent weights were not taken. All animals were observed for signs of toxicity as well as changes in appearance and demeanor. Chambers, Vapor Generation, and Analysis 3 The exposure chambers were 1 m stainless steel and glass Rochester-type chambers. Airflow through the chambers was maintained at 150 liters per minute. The air supply system is designed to provide chamber temperatures of approximately 21C and 50% relative humidity. The VC gas was metered through a calibrated flowmeter at a controlled rate into the main chamber air supply and further diluted to the desired concentration. DO 137966 CONFIDENTIAL -5- The analytical concentration of VC in each chamber was determined by infrared spectophotometry using a Mlran I infrared spectrophotometer equipped with a variable pathlength gas cell. Analyses were performed at a wavelength of 10.9u at least 10 times daily for each exposure chamber. Standards for the analyses were made by Injecting a known volume of VC gas into a 100 liter Saran gas sampling bag filled with a known volume of air. Exposure Regimen The groups of animals as detailed in the general study design were exposed to 0 (control), 1000, or 5000 ppm VC for 6 hours per day. These exposures were to be performed 5 days/week. However, in Study A, the exposures were terminated after 4 consecutive days. In Study B, the animals were exposed for 5 consecutive days. Microbiological Determinations Blood samples were collected from anesthetized animals from Study A for serological determination of antibodies to several murine viruses commonly found in laboratory rodents. Samples from representative animals from each exposure level/species were taken 5 days after termi nation of the exposures. Additional (convalescent) serums were collected from mice 13 days after the termination of exposures. The serums were sent to Microbiological Associates, Bethesda, Maryland for their standard panel of murine virus antibody determinations. Included in this panel were pneumonia virus of mice, reovirus 3, encephalomyelitis virus, K virus, polyoma, minute virus, ectromelia, Sendai, mouse adenovirus, mouse hepatitis, lymphocytic choriomeningitis, and rodent corona. Necropsy and Gross Pathology All animals presented to necropsy alive were anesthetized with methoxyflurane and the animal was killed by decapitation. Immediately after DO 137967 CONFIDENTIAL -6- decapitation, Che eyes were examined In situ by gently pressing a glass slide against the cornea and examining the eye under fluorescent light illumination. A gross necropsy examination, both externally and internally, was performed with all alterations recorded. Representative portions of the following organs and tissues were preserved in 10Z, neutral, phosphatebuffered formalin from each mouse: adipose tissue adrenal glands accessory sex glands aorta bone with bone marrow (thoracic vertebral column) brain epididymides esophagus eyes gallbladder heart kidneys lacrimal (Harderian) gland large intestine (cecum and colon) larynx liver lower Jaw lungs lymph nodes (thoracic, mesenteric) mammary tissue pancreas peripheral nerve (sciatic) pituitary gland salivary gland (submandibular) skeletal muscle skin small intestine spinal cord (thoracic) spleen sternum stomach testes thymus thyroid (with parathyroid) tongue trachea urinary bladder all gross lesions Only livers and kidneys were preserved from three of the hamsters and rats. For Study A, two mice/exposure group were sacrificed three days after termination of exposures. Two days later three animals/species/exposure group were sacrificed. The remaining animals were held until 13 days after the termination of exposures, at which time they were sacrificed by decapitation following methoxyflurane anethesia. Because of the lack of further clinical signs of disease or mortality considered due to VC exposure, necropsies were not performed on these animals. However, the abdominal and thoracic viscera from all mice were exposed and exam ined and the livers from six mice were retained in formalin. DO 1.37968 GONFI DENT I Al -7- An external and Internal necropsy examination was performed for all animals that died spontaneously during the course of the studies. This consisted of four mice exposed to 1000 ppm VC dying after the third or fourth exposure in Study A, one mouse exposed to 5000 ppm dying 13 days after termination of exposures in Study A, and one mouse exposed to 1000 ppm dying after 2 exposures in Study B. These animals were refrigerated from the time death was noted until necropsy. The tissues and organs listed above were retained in 10Z, neutral, phosphate-buffered formalin. Histological Preparations Hematoxylin and eosln-stained sections of paraffin-embedded tissues were prepared by the standard operating procedures of the histology laboratory An extensive set of tissues, intended to include all the major soft tissue organs, was prepared from the six moribund or dead mice after 3 or 4 exposures in Study A. The liver, gallbladder, thymus, and kidneys were prepared for histopathological examination from all mice from both studies except those mice from Study A terminated at 3 and 13 days following the final (fourth) exposure. From these mice, only the livers with gallbladders were prepared for histopathological examination. The four largest lobes of the liver (left lobe, right lobe and both portions of the medial lobe) were routinely prepared for histological examination. The livers, gallbladders (hamsters), thymus, and kidneys were prepared for histopathological examination from all rats and hamsters necropsied. For selected tissues, special-stained histological preparations (Giemsa stain. Von Kossa calcium stain, Dahl's calcium stain) were prepared by standard histopathology laboratory procedures (Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 1968). DO 137969 CONFIDENTIAL -8- RESULTS Chamber Analysis The dally mean analytical concentrations of VC were calculated from multiple dally chamber analyses. For Study A, these analytical concen trations were 96833 ppm and 5137165 ppm VC for the 1000 and 5000 ppm groups, respectively. For Study B, these values were 99945 ppm and 5112+173 ppm VC. Animal Observations The morbidity and mortality data from these two studies are summarized in Table 1. Study A: After 3 exposures, some of the mice exposed to either 1000 ppm or 5000 ppm VC were lethargic. Their coats were roughened and some had distended abdomens. On the morning following the third exposure (prior to the fourth exposure) 3 mice exposed to 1000 ppm were dead and 3 more were moribund. During the fourth exposure 1 of these moribund mice died. The hamsters and rats appeared normal. At this time, the expo sures were terminated and all animals were held for further observation. The two moribund and four dead mice exposed to 1000 ppm VC were presented for necropsy examination. Three days after termination of the exposures, two more mice from each group were necropsied. Clinically, these animals were all active but one mouse exposed to 5000 ppm VC had a distended abdomen. Five days after termination of the exposures, three more mice and three rats and hamsters from each group were necropsied. These mice were active but two of the animals exposed to 5000 ppm VC had distended abdomens. The remaining animals were observed for 13 days. During this interval, clinical abnormalities were not noted; however, one mouse exposed to 5000 ppm VC was found dead thirteen days after the final exposure. DO 137970 CONFIDENTIAL -9- The rats and hamsters remained normal throughout the 2 week observation period. Three animals/exposure level/species were sacrificed five days after the fourth exposure. Study B: The clinical observations of the mice In Study B were similar to those noted In Study A. One mouse exposed to 1000 ppm died after two exposures. Some mice exposed to 1000 ppm and 5000 ppm VC were lethargic and had roughened pelage after three exposures. Distended abdomens were noted after three or four exposures for 3 of the 4 remaining mice exposed to 1000 ppm and 2 of the 5 mice exposed to 5000 ppm VC. Microbiological Determinations To investigate the possible presence of viral Infectious disease, serological examinations were performed on specimens collected from mice, rats, and hamsters from all exposure groups in Study A five days after the last (fourth) exposure. Convalescent titers from mice were analyzed on samples taken 13 days after the last exposure. The titers are presented in Table 2. Antibodies to 12 different murine viruses were not detected in mice at any exposure level at either sampling time. Hamsters and rats had antibodies to pneumonia virus of mice (PVM) and Sendai virus. The antibody level in rats to these viruses was low. In hamsters, antibody titers to Sendai virus were high, whereas those to PVM were low. Gross Pathology The gross lesions noted in Study A are presented in Tables 3 (mice), 4 (hamsters), and 5 (rats). The gross lesions noted in mice from Study B are presented in Table 6. DO 137971 OONFTDFNTTAl -10- The only gross lesions considered to be directly related to the Inha lation of vinyl chloride were noted In mice from both studies. Because of the Irregular schedule of termination with the accompanying variable, but small, group size and also because the lesions noted were similar in both studies, the results of the gross pathologic examination will be presented without regard to study but with Interpretation allowing for the temporal responses of the recovery period. Externally, a low number of mice exposed to either 1000 or 5000 ppm VC developed distended abdomens. This was noted in animals presented alive to necropsy after 4 or 5 exposures and was still present In some animals 3 to 5 days after termination of exposure; however, this was not noted in animals dead or moribund after 2-4 exposures. One of the five animals exposed to 5000 ppm VC for 4 exposures and allowed a 5-day recovery period also had ventral subcutaneous edema noted externally. The animals dying spontaneously after 3 or 4 exposures to 1000 ppm VC had reddened nares considered to represent agonal congestion. Internally, some mice exposed to either 1000 or 5000 ppm VC had gross lesions suggesting toxicity to the liver, lymphoid system, and possibly cardiovascular system. In the affected mice, the livers were usually enlarged and had a variety of color changes. Frequently there was hepatic congestion that varied from generalized to a unique pattern of central or hilar congestion with pale or blanched margins. The hepatic lobular pattern was often accentuated, apparently due to congestion. Foci compatible with acute necrosis were noted. These varied from large foci up to several mm in size, occupying most of a liver lobe and varying in color from pale to red, to multiple pinpoint pale foci. These necrotic-appearing areas were generally larger and involved more of the liver in those mice dying or moribund after 2-4 exposures to 1000 ppm VC than in mice surviving the exposure periods. DO 137972 CONFIDENTIAL -11- Clear to yellow ascitic fluid and subcutaneous edema was also generally noted in the mice having liver lesions. The mice exposed to 1000 ppm VC had, generally) slightly more severe fluid accumulations than those exposed to 5000 ppm VC. The animals dying spontaneously frequently had pulmonary lesions charac terized by slight pulmonary edema and slight to moderate accumulations of serous fluid in the thoracic cavity. Since this was not noted in animals surviving the exposure period, it is believed that this repre sents agonal cardiovascular collapse. A slight decrease in the size of the thymus was noted In all four mice surviving five exposures to 1000 ppm VC (Study B) and 3 of 5 surviving five exposures to 5000 ppm VC. The other gross lesions presented in Tables 2 and 5 are considered to be either secondary non-specific lesions commonly noted in moribund animals, changes associated with the necropsy procedures, or spontaneous lesions frequently noted in mice of this age and strain. The one mouse found dead 13 days following termination of exposure to 5000 ppm VC was moderately autolytic but no other lesions were noted. The gross observations noted in hamsters (Table 4) and rats (Table 5) from Study A were all considered to be artifacts induced by necropsy procedures or spontaneous lesions frequently noted for these species. His topa thology The histopathologic observations made in Study A are presented in Tables 7 (mice), 8 (hamsters), and 9 (rats). For the mice from Study B, the histopathologic observations are presented in Table 10. The histopathologic examination of tissues from these animals confirmed the gross observations of liver, lymphoid, and possibly cardiovascular toxicity in mice. As was done in the section on gross pathology, the DO 137973 CONFIDENT! Al -12- histopathologic observations will be discussed by combining both studies. The lesions were similar In the two studies when allowance Is made for the varying time period from termination of exposure until necropsy. Mice dying spontaneously or moribund after 2-4 exposures to 1000 ppm VC were characterized by acute hepatic necrosis affecting a large portion of the liver. In the seven mice with this exposure history, about onefourth of the liver area was necrotic In one mouse, between onefourth and one-half was necrotic In four mice, between one-half and threefourths was necrotic In one mouse, and the remaining mouse had necrosis of over three-fourths of the liver as judged from histologic sections of the four major liver lobes. When the necrotic areas were small, they were centrllobular; however, they were frequently massive and involved confluent lobules with only a thin rim of viable hepatocytes around the portal triads. Frequently, minute basophilic intracellular granular structures were noted in swollen necrotic hepatocytes as well as viable hepatocytes at the margins of the necrotic areas. These were demon strated to contain calcium by special staining techniques. There was minimal inflammatory reaction noted at the margin of the necrotic areas in these mice dying spontaneously. The few inflammatory cells present were predominantly macrophages. There were marked hepatic vascular changes noted in this group of mice. There was usually intense con gestion within and at the margins of the necrotic areas. In the areas of viable hepatic parenchyma there was often severe dilatation of the centrllobular vein with compression of adjacent parenchyma along with focal areas of sinusoidal dilatation scattered throughout the hepatic lobule. These areas often appeared as vascular "lakes" among the parenchyma. The viable areas in the livers of these animals had centrilobular or perlnecrotlc hepatocytes generally characterized by swelling with vesicular or vacuolated cytoplasm. In some livers, these centrilobular or perlnecrotlc hepatocytes had increased cytoplasmic homogen eity and basophilia with occasional mitotic figures. A low number of small, eosinophilic, sinusoidal masses were frequently seen 00 137974 confidfnttal -13- among these cells. Uhlle this material could represent necrotic cellu lar debris, these masses vere considered more likely to be microthromboses The livers of mice surviving 4 or 5 exposures to 1000 or 5000 ppm VC had marked hlstopathologlcal variability. The four mice sacrificed Immediately after completion of 5 exposures to 1000 ppm VC (Study B) had acute centrllobular hepatic necrosis. While this was qualitatively similar to that noted for those animals dying spontaneously at this exposure level, It was much less In degree -- being about one-tenth of the liver In three of these mice and one-fourth In the other one. These necrotic foci were often present In a subcapsular location. The reaction to the necrotic foci was similar to that described for those animals dying spontaneously but the vascular reaction was less dramatic and the Inflammatory reaction Included slight connective tissue proliferation. In centrllobular areas lacking necrosis, the hepatocytes often were enlarged with Increased cytoplasmic homogeneity and basophilia and occasionally cytoplasmic vacuolization and mitotic figures. Hyaline sinusoidal masses consistent with microthrombi were seen in some of the centrllobular areas. The five mice sacrificed Immediately after completion of 5 exposures to 5000 ppm VC (Study B) had marked variation in histopathologic changes. Three of these mice had only microvesiculation of the centrllobular hepatocytes but lacked any significant amount of necrosis other than the scattered microscopic foci typically noted also in control animals. The other two animals were similar to those animals dying spontaneously In that they had large areas of acute necrosis (between one-fourth and one-half of the liver in one mouse and between one-half and three-fourths in the other). The Inflammatory and vascular events roughly paralleled those noted in the mice dying spontaneously with comparable liver lesions. DO 137975 CONFTDFNTT AL -14- Ulch 3 to 5 days of recovery following completion of four exposures to vinyl chloride, there was again variability In the histopathologic changes In the liver. Three of five mice exposed to 1000 ppm VC and 2 of 5 mice exposed to 5000 ppm VC had changes of the centrilobular hepatocytes with either enlargement and cytoplasmic vesieulation or cytoplasmic homogeneity, basophilia, and occasional mitotic figures. In one mouse In each exposure level this reaction was more severe with marked cytoplasmic swelling and vesieulation, scattered necrotic indi vidual hepatocytes and sinusoidal thrombi and dilatation. In one mouse exposed to 1000 ppm VC and 2 mice exposed to 5000 ppm VC, there were multifocal areas of centrilobular hepatic necrosis involving about onetenth of the liver parenchyma. These were surrounded by a slight subacute inflammatory reaction in which fibroblasts were noted. In one mouse exposed to 5000 ppm VC, some multinucleate giant cells were noted in the reaction. By 13 days after completion of the exposures, the livers of 4 of 6 mice In each exposure group were similar to controls or had only slight microveslculatlon and enlargement of centrilobular hepatocytes. One mouse in the 5000 ppm VC exposure group had marked centrilobular hepatocyte enlargement with occasional mitotic figures and sinusoidal micro thrombi. Two mice exposed to 1000 ppm VC and one mouse exposed to 5000 ppm VC had multifocal areas of fibrosis with mononuclear inflammatory cells and, characteristically, multinucleate giant cells containing a deeply basophilic structure. This was identified as calcified material by appropriate staining techniques. These fibrotic areas were centri lobular and often subcapsular. These livers also had slight periportal accumulations of mononuclear inflammatory cells and slight bile duct hyperplasia was noted in one mouse exposed to 1000 ppm VC. Foci of dilated sinusoids surrounded by thin fibrous connective tissue walls were noted throughout the liver, particularly subcapsularly. The thymus had histopathologic lesions that closely paralleled the degree of hepatic necrosis. Mice that were moribund or died sponta neously had loss of the thymic lymphoid cells that varied but was DO 13797ft C0NFTDFNTTA1 -15- usually moderate to marked In severity. These also had karyorrhectlc nuclear fragments In the medulla of the thymic lobules. The thymuses of mice that survived the exposure period were frequently normal. When depletion of thymic lymphocytes was noted In surviving mice It was present in the mice having hepatic necrosis. The severity of thymic lymphoid depletion roughly paralleled the severity of hepatic necrosis. In the mice dying spontaneously, the other lymphoid tissues examined (spleen and lymph nodes) had decreased lymphoid cells similar In degree to that noted In the thymus. Histopathologically, the kidneys of mice dying spontaneously frequently had tubules with sloughing epithelium, pyknotic nuclei, and hypereosinophllic cytoplasm. As these changes were not noted in properly fixed kidneys from sacrificed mice they were attributed to autolysis. Three of the five mice exposed to 5000 ppm VC (Study B) had slight to moderate cytoplasmic microvesiculation of the renal cortical tubules. All other histopathologic observations made on the kidneys of treated or control mice were considered to be spontaneous entities and not due to exposure to vinyl chloride. The hearts of 3 of the 6 mice moribund or dead after A exposures to 1000 ppm VC (Study A) had isolated focal areas of minute basophilic structures within degenerating cardiac myocytes. This was shown to be calcified material by use of special histologic staining techniques. Other organs examined from mice dying spontaneously (Study A) had occa sional lesions. These were generally considered to be either secondary to the agonal condition of the mouse or spontaneous entities not due to VC exposure. The histopathologic observations noted for the liver, gallbladder, kidneys, and thymus from hamsters (Table 8) and rats (Table 9) exposed to vinyl chloride were considered to be spontaneous lesions with no lesions ascribed to VC exposure. DO 137977 OONF T DFNTI Al -16Histologlc examination of the liver and kidneys from the one mouse found dead 13 days after termination of exposure to 5000 ppm VC disclosed moderate autolytlc changes. Ho lesions similar to those present In the affected mice were noted. Although a cause of death was not determined. It was felt that this mouse did not die from VC-induced hepatotoxicity and It was not included in the gross and histopathology tables (Tables 3 and 7). DO 137978 CONFIDENTIAL -17- DISCUSSION The acute deaths In mice exposed to 1000 ppm VC were first suspected to be of infectious origin. Early results from histopathological exam ination combined with results of viral serology failed to support this presumption. Neither viral inclusion bodies nor bacteria were noted on histopathological examination. Mice failed to have titers to numerous viral pathogens, including those commonly associated with hepatic necrosis (ectromella, mouse hepatitis virus, and reovirus). Although antibody titers to two viruses were present in both rats and hamsters, the titers were relatively low and the viruses both produce pneumonia rather than hepatic disease. These findings suggested that VC may be acutely toxic to the male mouse. Although hindered by the low numbers of mice, the marked variation in severity of lesions, and the variable degree of autolytic change, the staggered necropsy times following termination of VC exposure allowed a morphologic assessment of the nature and progression of lesions in mice following short-term inhalation exposure to VC. The major organ affected was the liver. The first histological changes appeared to be vesiculation, vacuolation and swelling of the centrilobular hepatocytes. This was noted in most of the animals exposed to either 1000 ppm or 5000 ppm VC. In a low proportion of animals this lesion was markedly more severe with marked centrilobular and midzonal cellular enlargement and vacuolization and compression of the sinusoids. In these areas small hyaline masses were frequently noted among the swollen cells. These masses could represent necrotic hepatocytes but are considered more likely to be microthromboses. Following the initial cytoplasmic changes, the centrilobular and midzonal hepatocytes in some livers had homogeneous eosinophilic cytoplasm with changed staining affinity characterized by a slight basophilic tinge. 00 337979 confident IA| -18- Xhese cells were enlarged, sometimes markedly, and mitotic figures were often noted. As this change was more prominent in the animals sacri ficed after several days of recovery, this was considered a regenerative response. Next in the apparent progression of lesions was centrilobular necrosis. This was characteristically coagulation necrosis with only a very slight peripheral inflammatory reaction. The inflammatory reaction that ensued consisted primarily of macrophages and fibroblastic elements. The progression from the centrilobular hepatocyte swelling to necrosis was unclear. Frequently the perinecrotic hepatocytes were either vacuolated or contained eosinophilic intracytoplasmic masses assumed to be degenerated cystosol proteins. However, these changes in the hepatocytes adjacent to necrotic areas were of no greater severity than those present in cells in non-necrotlc centrilobular areas. Thus, degree of vacuolar change alone did not appear to correlate with the necrosis. Of interest are the minute basophilic structures noted in some of the necrotic hepatocytes and perinecrotic hepatocytes. These were demon strated to contain calcium via the use of special histological stains. It is speculated that these structures represent mineralized mito chondria. Mitochrondrial calcification is commonly noted by agents primarily attacking the cell membrane (Trump and Arstila, 1975). In all mice dying after 2-4 exposures to 1000 ppm VC, there was progression from multiple small centrilobular necrotic areas to massive confluent areas of necrosis. Most animals dying spontaneously had necrosis of about 50% of the hepatic area on the four liver sections examined. Survivors generally had necrosis of 25% or less of the liver area. With mice exposed to 5000 ppm VC, there were no deaths ascribed to VC and the amount of liver necrosis was generally less. However, in two mice exposed to 5000 ppm VC for 5 exposures (Study B), there was massive necrosis (up to 75%) and it is speculated that death may have been imminent had the animal not been sacrificed. r: 137980 ONFTDFNTTAI -19- The role of the vascular system In the pathogenesis of the liver necrosis Is uncertain. In the animals dying spontaneously there was dramatic congestion In and adjacent to the necrotic areas. There was frequently marked congestion of the central vein and focal areas of sinusoidal dilatation with compression of adjacent hepatocytes. There was slight to moderate pulmonary congestion and edema; however, other abdominal organs (spleen, kidneys, gastrointestinal tract) had little evidence of vascular congestion to suggest generalized heart failure. Although sinusoidal microthrombi were present, larger thrombi were not noted. Survivors frequently had small sinusoidal dilatations surrounded by fibrous connective tissue, but lacked other vascular changes. The dramatic vascular congestion noted in the livers of moribund or dying animals was considered to be a probable secondary reflection of the liver necrosis as well as terminal heart failure. In a recent paper reviewing human hepatic angiosarcomas due to vinyl chloride and other causes. Popper et al. (1978) note a precursor stage characterized by hepatocellular proliferation along with sinusoidal dilatation and sinusoidal lining cell proliferation. Their early lesions are similar to those described in this report; however, their report does not note hepatic necrosis, but rather the progressive atrophy and disappearance of the hepatocytes with increasing prolif eration of the sinusoidal lining cells leading to angiosarcoma. Also prominently affected by short-term inhalation exposure to VC was the lymphoid system, particularly reflected by the degree of thymic lymphoid necrosis and atrophy. The degree of thymic lymphoid depletion generally paralleled the degree of liver necrosis but appeared to lag temporally slightly behind the liver necrosis. This was noted espe cially in survivors in which there were slight thymic changes in spite of liver necrosis. It is speculated that the lymphoid depletion is secondary to the liver necrosis, possibly due to elevated levels of adrenal corticosteroids produced in response to systemic stress due to the massive hepatic necrosis. OO CONF 137981 rFNTTA!.. -20- There were several changes suggestive of possible disturbed mineral metabolism In the mice with liver necrosis. In addition to the lntracytoplasmic mineral noted In and adjacent to the necrotic liver areas, mineral deposits were noted In foci In the myocardium and larger bodies In necrotic areas of the thymus. It Is speculated that the mineral ization Is dystrophic due to cellular injury rather than a reflection of calcium-phosphorous imbalance. The kidneys had only slight changes considered to be insignificant in causing the death of these mice. The tubular changes noted in the mice dying spontaneously were regarded as artifacts due to autolysis. With sacrifice and prompt fixation, the only lesion attributed to VC exposure was slight to moderate mlcroveslculatlon of the cortical tubular epithe lium. Surprisingly, the acute hepatotoxic effect of VC In mice has apparently not been generally recognized. Early short-term inhalation experiments noted that 30 minute exposures to 300,000 ppm VC were lethal to mice and rats, but 100,000 ppm produced only narcosis (Mastromatteo et al. 1960). Prodan et al. (1975) calculated the LC^q for white mice as 11.75% VC in a ventilated chamber. A dominant lethal test In CD-I male mice (the same strain as that used in this report) exposed by Inhalation to 3,000, 10,000, or 30,000 ppm VC for 5 daily 6-hour exposures was reported by Anderson et al. (1976). These doses were selected on the basis of their unreported probe tox icity studies which determined 30,000 ppm to be In the toxic range (the criteria of toxicity were not stated). In the published work, Anderson e^ al. had significant mortality (55%) at the 30,000 ppm exposure level and 5% and 10% mortality at the 10,000 ppm and 3,000 ppm exposure levels, respectively. The cause of death was not stated. DO .1.37982 oonftdenttai -21- There have been several subchronic or chronic toxicity studies utilizing mice exposed to VC at levels close to those used In this report. Winell et al. (1976) exposed NMRI (albino) mice to 50 or 500 ppm VC. After 40 weeks the serum levels of lactate dehydrogenase and alkaline phosphatase were Increased. Although these enzymes may indicate liver damage, they were apparently Increased only after liver tumors were present. There is no indication of any acute deaths due to VC exposure in their report. Carcinogenesis studies on VC In mice have been reported by Maltoni and Lefemine (1975), Keplinger et al. (1975), and Lee et al. (1977). In all these studies, the mouse was markedly more sensitive than the rat. Maltoni and Lefemine exposed Swiss mice 4 hours/day, 5 days/week for 30 weeks to levels of 50, 250, 500, 2500, 6000, or 10,000 ppm VC. Although no acute lethal effects of VC were presented, there appears to be slight excess mortality in male mice at the higher exposure levels as judged by the number of survivors at 16 weeks. Keplinger at al. exposed CD-I mice to 50, 200, or 2500 ppm VC for 7 hours/day, 5 days/week for 9 months. The interim data reported after 8 months of exposure indicated several mice with tumors but there was no indication of acute hepatoxicity. The only published report noting acute hepatotoxicity in mice is that of Lee et al., in which CD-I mice were exposed to 50, 250, or 1000 ppm VC for 6 hours/day, 5 days/week for 12 months. They report observations strikingly similar to chose noted in this report. Two males and one female (from group size of 36/sex) were found dead between the third and ninth exposure to 1000 ppm VC. Histopathology disclosed acute toxic hepatitis characterized by diffuse coagulation necrosis beginning in the centrilobular areas and focal to marked congestion. They also reported renal tubular necrosis characterized by pyknosls and eosinophilic granulation of the cytoplasm. Their research protocol included the sacrifice of 4 mice/sex at the end of 1, 2, and 3 months. Review of DO 137983 CONFIDENTIAL -22- their progress report (No. 8 submitted to NIEHS) discloses no reported histological lesions for these 24 mice other than minor lesions also present In the control animals. The studies reported herein confirm the acute hepatotoxiclty of VC to CD-I mice as first noted by Lee et al. and present a more detailed hlstopathologlcal description of the associated lesions. Several note worthy aspects of this response are presented Including: 1. VC appears to be acutely hepatotoxic at these relatively low concentrations only In the mouse, particularly the CD-I strain of mice. 2. There is a consistent association of greater severity of toxicity by virtually any of the parameters effected (mortality, gross lesions, or histologic lesions) In the lower exposure level of 1000 ppm VC as opposed to those in the higher exposure level (5000 ppm). 3. There Is wide variability within a treatment group; l.e., up to 75% of the liver necrosis and death in some mice exposed to 1000 ppm VC while others survive with virtually undetectable histological lesions. It is concluded that the observations reported herein need consideration when evaluating research involving vinyl chloride exposure of mice. DO 1.37984 CONFIDENTIAL t -23- This report was prepared and submitted by the following Staff Members: ____________________ Keith A. John6bn, D.V.M., Ph.D. Diplomate, American College of Veterinary Pathologists Project Leader H7 0. lakel, B.S. Senior Research Biologist Inhalation Toxicology Date: 3 Date Reviewed by: *---________________ M. J.McKenna, Ph.D. Diplomate, American Board of Toxicology Group Leader, Inhalation Toxicology Date: 00 137985 CQHFlnNTTAL 24 QUALITY ASSURANCE STATEMENT VINYL CHLORIDE: RESULTS OF TWO TITLE OF STUDY: SHORT-TERM INHALATION STUDIESHT-K-1711-(29) In compliance with Good Laboratory Practice Regulations, the study phases were inspected by the Quality Assurance Unit and the results of these inspections reported to Management and the Study Director on the dates listed below. The report accurately reflects the data generated in accordance with the regulations and standard operating procedures of the laboratory. All data and the reports are located at the submitting laboratory. Study Started: Dates of Inspection: 30 Oct. 1979 19 Oct. 1979 1 Nov. 1979 31 Jan. 1980 3 June 1980 16 Jan. 1981 Report Issued Date: 3 Feb. 1981 Date of Report: 19 Oct. 1979 2 Nov. 1979 5 Feb. 1980 5 June 1980 22 Jan. 1981 'Hd / WswcAJ 3/f/Law /??/ W. E. Hoover ~~ Quality Assurance Toxicology Research Laboratory Health & Environmental Sciences 18Q3 Building Dow Chemical U.S.A. Midland, MI 48640 me DO 137986 CONFIDENTIAL. -25- REFERENCES Anderson, D., Hodge, M.C.E., and Purchase, I.F.H. Vinyl Chloride: Dominant Lethal Studies in Male CD-I Mice. Mut. Res. 40:359-370. 1976. Cortes, H. J. Purity of Vinyl Chloride for Animal Inhalation Study. Analytical Report, 1979, Midland Analytical Laboratories, The Dow Chemical Co. Keplinger, M. L., Goode, J. W., Gordon, D. E., and Calandra, J. C. Interim Results of Exposure of Rats, Hamsters, and Mice to Vinyl Chloride. Ann. N.Y. Acad. Sci. 246:219-224, 1975. Lee, C. C., Bhandarl, J. C., Winston, J. M., House, W. B., Peters, P. J., Dixon, R. L., and Woods, J. S. Inhalation Toxicity of Vinyl Chloride and Vinylidene Chloride. Environ. Health Perspect. 21:25-32, 1977. Maltoni, C., and Lefemine, G. Carcinogenicity Bioassays of Vinyl Chloride: Current Results. Ann. N.Y. Acad. Sci. 246:195-218, 1975. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. L. G. Luna, editor. McGraw Hill Book Co., New York. 3rd ed., 1968. Mastromatteo, E., Fisher, A. M., Christie, H., and Danziger, H. Acute Inhalation Toxicity of Vinyl Chloride to Laboratory Animals. Am. Ind. Hyg. Assoc. J. 21:394-398, 1960. Popper, H., Thomas, L. B., Telles, N. C., Falk, H., and Selikoff, I. J. Development of Hepatic Angiosarcoma in Man Induced by Vinyl Chloride, Thorotrast, and Arsenic. Am. J. Pathol. 92:349-376, 1978. 00 137987 CONFTDFNTT Al -26- REFERENCES (Continued) Prodan, L., Suciu, I., Pislaru, V., Ilea, E., and Pascu, L. Experimental Acute Toxicity of Vinyl Chloride. Ann. N.Y. Acad. Sci. 246:154-158, 1975. Stott, W. T. Protocol for the Investigation of the Mechanism of Tumor Initiation by Vinyl Chloride, 1,4-Dioxane, and 1,3-Hexachlorobutadiene, 1979. The Dow Chemical Co., Midland, Michigan. Trump, B. F., and Arstila, A. U. "Cellular Reaction to Injury" in Principles of Pathoblology. pps 9-96, 2nd ed., (1975). Edited by LaVia, M. F. and Hill, R. B. Oxford University Press, New York. tflnell, M., Holmberg, B., and Kronevl, T. Biological Effects of Vinyl Chloride: An Experimental Study. Environ. Health Perspect. 17:211-216, 1976. DO 137988 CONFIDENTIAL -27- TABLE 1 VINYL CHLORIDE: RESULTS OF TWO SHORT-TERM INHALATION STUDIES Morbidity and Mortality Exposure Level (ppm)_________________ STUDY A - 4 exposures Mice - Number Exposed Number Died (Number of exposures before death) Number moribund or with distended abdomens (Number of exposures) Hamsters - Number Exposed Number Died Number Moribund Rats - Number Exposed Number Died Number Moribund __ 0 30 0 0 10 0 0 10 0 0 1000 30 4 (3,3,3,4) 2 (4,4) 10 0 0 10 0 0 5000 30 <4a) v2 (4,4b) 10 0 0 STUDY B - 5 exposures Mice - Number Exposed Number Died (Number of exposures before death) Number with distended abdomens (Noted at necropsy after 5 exposures) 5 0 0 55 10 (2) 32 This animal was found dead 13 days after exposures were terminated. bThese animals were noted with distended abdomens 3 and 5 days after the exposures were terminated. - Indicates not applicable. 137989 LONEIDENT TAI Species House House Mouse House House House llaaster Haiss ter Haaster Rat Rat Exposure Level (ppm) a 1000 5000 0 1000 5000 0 1000 5000 0 1000 TABLE 2 Tie of Saaple 5 5 5 VINYL CHLORIDE: RESULTS OP TWO SHORT-TERM INHALATION STUDIES Viral Antibody Titers Proa Nice, Haastera, and Rats _____________________________________________________Antibody Titer to: PVH Reo 3 CDV II K Polyoaa Minute Extreme] la Sendai __ __ _ _ -- -- - - - - -- -- - - - - Adeno - Hepatitis House - l.CH - Rodent Corona - - 1] - - -- _ _ - -- - - HD 13 - - -- - - - -- - - HO 13 - - -- - - - -- - - HD 5 -S 40 5 20 - -- -- - - - - - >B0 - - ^>80 - - 40 - - -- -- - -- 5 40 5 20 - --- _ _ - 10 _ -a - 10 - _ m. 00 137990 CONFTDFNTTAI aDays following teruination of exposures. - Indicates no antibodies to the stated virus were present. HD " Hot Done So Zo n TABLE 3 VINYL ClILOlUDE: RESULTS OR TWO SIIORT-TERH INHALATION STUDIES Cross Pathologic Observation - Study A Hlce Exposure Level (]'|iw) f) Necropay Group Huabur of Mice In Croup 0 3 or 5 Days Recovery s Moribund or Dead after ^ 3-4 Exposures" 6 1000 2 or 5 Days Recovery 5 13 Days Recovery 6 EXTERNAL OBSERVATIONS No visible Jealous 01scended abdomen Subcutaneous dess Reddened narcs Focal eaiiiubdlJss INTERNAL OBSERVATIONS Hu visible lesions General Ventral subcutaneous edema - Moderate to Marked Ascites - clear abdoMlnal fluid - Moderate to Marked Obeae Liver Generalised enlargement or swelling Lobar or large oreaa of color change, suggesting neurosis (either pale or reddened) Pinpoint pale foci, multiple, suggesting focal necrosis Congestion or reddened Accentuated reddening of hilar areas of multiple lobes Pale lobar margins, focal or diffuse Multiple snail red foci increased lobular pattern Generalized paleness S 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 1 2 0 0 4 1 0 0 0 1 5 2 0 4 0 2 1 5 0 Data presented are number of mice having the stated observation* aCrouped by days after final (fourth) exposure when necropsled. ^Includes i dead after three exposures and 1 dead and 2 moribund after four exposures* s 0 0 0 0 0 .0 0 0 1 0 0 1 1 1 1 2 0 6 0 0 0 0 3 0 0 0 0 0 1 0 0 0 0 0 0 WOO 3 or S Days Recovery s 13 Days Recovery 6 26 30 10 00 00 1S 20 20 00 00 11 10 00 10 00 00 30 00 r TAHI.E 3 (Continued) VINYL CHLORIDE; RESULTS OF WO SHORT-TERM iNUAIATlON STT1UIES Crons Pathologic Observation - Study A Mice oo Zn o ?--( ^ o co rn si z -o --f -o \> T-- Exposure Level (pint) s Necropsy Cron|> __ _ Number of Mice lu Croup Umgs CoDgeuUJ, diffuse Edema - alight Focal areas of espltyuau Multiple red foci consistent with aspirated blood Tlioracic Cavity Clear fluid - slight to Moderate Mesentery Edema Stomach Caseous distention Congested or reddened Small Intestine Congested or reddened Decreased ingests Kidney Congested or reddened Thymus Petechial hemorrhage 3 or 5 Days Recovery 5 0 0 0 2 0 0 0 0 0 0 1 0 Moribund or Dead after ^ 3-4 Exposures 6 1UW 3 or S Days Recovery 5 13 Days Recovery 6 4 00 1 00 1 00 0 50 6 10 1 00 1 00 2 00 1 00 2 00 0 0U 0 10 Data presented ore musber of nice Itavlng the etated observation. "cruuped by doys after final (fourth) exposure when netropeled. ^Includes 3 dead after three exposurea and 1 dead and 2 Moribund after four exposures. MOO 3 or 5 Days Recovery s 13 Days Recovery 4 00 10 00 30 00 00 00 00 a0 a0 00 00 u ? 31- TABLE 4 VINYL CHLORIDE: RESULTS OF TWO SHORT-TERM INHALATION STUDIES Gross Pathologic Observations - HamstersB Exposure Level Number Examined____________________________ EXTERNAL OBSERVATIONS No visible lesions Firm enlarged boney prominence on lower left rear leg 0_ J3 3 0 1000 3 3 0 5000 ___ 3 2 1 INTERNAL OBSERVATIONS No visible lesions 10 1 Lungs Multifocal red areas consistent with aspirated blood at necropsy Focal, depressed, dark red area, isolated 12 10 1 0 Liver Slight accentuation of lobular pattern 0 2 1 Tibia Firm smooth boney mass, distal tibia; consistent with healed fracture 0 0 1 Data presented is number of hamsters with stated observation. aHamsters were necropsied 5 days after fourth exposure to vinyl chloride. DO 137993 CONFTDENTTAI -32- TABLE 5 VINYL CHLORIDE: RESULTS OF TWO SHORT-TERM INHALATION STUDIES Gross Pathologic Observations - Ratsa Exposure Level (ppm)_________________________________ Number Examined EXTERNAL OBSERVATIONS No visible lesions () ,3 3 10Q0 3 3 INTERNAL OBSERVATIONS No visible lesions Thymus Multiple petechial hemorrhages Lungs Multifocal red areas consistent with aspirated blood 02 11 20 Data presented is number of rats with stated observation. aRats were necropsied 5 days after the fourth exposure to vinyl chloride. 00 A 37"^ A\CONF * * 33- TABLE 6 Vim CHLORIDE: RESULTS OP TWO SHORT-TERM INHALATION STUDIES Croat Pathologic Observations - Study ! Mice Naeroeav Grouo Number of Mica In Grouo Final Termination* 'S EXTERNAL OBSERVATIONS No vlalbla laalona Cornaal cloudineae, focal - unllataral - bLLataral Dlatandad abdoman Small alza Clinically dapraaaad 4 0 1e 0 0 0 INTERNAL OBSERVATIONS No vlalbla laalona Ganaral Vandal aubcutanaoua adama - markad - modaiata - alight Ascites, claar abdominal fluid - markad - modarata Dacraaaad abdominal fat 3 0 0 0 0 0 0 Llvar Ganarallzad anlargamant Lobar or larga araaa of color changa (althar pala or raddanad) Pinpoint pala foci, miltipla Accancuacad raddanlng of hilar araa of multiple lobaa with pala lobar margina Accentuated lobular pattern Ganarallzad palanaaa 0 0 0 0 0 0 Lunna Congested, conslatent with hypostatic congaatlon Pinpoint pink foci, few, indistinct Multiple rad foci consistent with aspirated blood 0 1 1 S tomach Submucosal edema, glandular portion - alight Multifocal pinpoint dark brown foci. glandular mucosa 0 0 Small Intestine Decreased Ingests 0 Cacum Incraasad consistency of Ingests 0 Thvmus Dacraaaad size - alighc 0 Vertebrae Prominent thoracic Intervertebral dlsca 2 Exposures lb 1000 Pinal Tarminatlon 4 1i 00 00 03 01 00 00 01 01 01 03 00 01 13 02 03 10 12 02 10 00 03 01 00 10 1 0 04 5000 Final Tarminatlon 3 2 1 0 2 0 1 0 0 Q 0 1 1 4 2 2 0 2 2 0 0 0 2 0 1 0 0 3 Data presented ara number of anlmala having tha ataced obaarvation. *Naeroplad aftar flva exposures, b Animal dlad following two axpoauraa. Do 137995 CONFIDENT TAI. DO 1 3 7 9 9 6 CO NFIDENT! TAHI.E 1 VINYL CULORIDE: RESULTS OF TWO SHORT-TERM INHALATION STUDIES Histopathologic Obacrvatlona - Study A Mice Exposure level (ppm)_____________________________ _______________0 Necropsy Croup'1Recovery Humber of Mice in Croup_________________________ 1 or J Days ______ 5 Moribund or Dead after ^ 3-4 Exposures" 6 Liver (number examined microscopically) No visible lesions Acute coagulation necrosis, - focal, centrflobular, about 10Z of area of sections examined, often subcapsular - focal, ccntrllobular to confluent, Involving 15-25Z of area of sections examined - focal, centrflobular toconfluent, Involving 26-501 of area of sections examined - focal, cencrllobular toconfluent, involving 51-75Z of area of sections examined - massive, large areas of liver, greater than 751 of area of sections examined Minute, Intracellular basophilic structures at margins of necrotic areas Perl necrotic Inflammatory cells, mixed, - very slight to slight Perlnecrotlc subacute inflammatory and connective tissuecells - slight Perlnecrotlc swollen, vacuolated hepatocytea Contrllobular liepatocytea, mlcroveslcular change - very slight to slight - marked S 0 0 0 0 0 0 0 0 0 0 2 0 6 0 0 1 3 1 1 S 4 0 5 0 0 Data presented are number of animals having the stated observation. aCrouped by days after final (fourth) exposure when necropsled. kIncludes 3 dead after three exposures and 1 dead and 2 moribund after four exposures. 1000 3 or 5 Days Recovery 5 5 0 1 0 0 0 0 0 0 1 1 2 1 13 Days Recovery 6 6 0 1 0 0 0 0 0 0 2 0 3 0 5000 3 or 5 Days Recovery 5 5 0 13 Days Recovery 6 6 0 21 00 00 00 0 0 0 2 1 0 1 0 0 0 2 0 6 1 DO 1 3 7 9 9 7 CO NFIDENTIAL TAULE 7 (Continued) Vtim, CHLORIDE: RESULTS OF TWO SHORT-TERM INHALATION STUDIES Cross Pathologic Observation - Study A Mice Exposure level (ppm)_____________________________ _______________Q 3 or 5 Days necropsy Croup8___________________________________ Recovery Huuher of Mice In Croup_________________________ _______________5 Liver (Continued) Centrllobular and uldzona1 hepatocytes enlarged, homogeneous cytoplasm of slightly Increased basophilia, occasional mitoses Focal subcupeular hepatocytc degeneration with vacuolization and hyaline arterial Hultinucleate giant cells containing calcified material Sinusoidal congestion and Itenorrhage, In and near necrotic areas - moderate - marked Centrllobular vein congestion, narked, conpression of adjacent hepatocytes Vascular ectasia, apparently mldzonal, with conpression of adjacent hepatocytes Focal telangiectasis Small hyaline masses in sinusoids, marginsof necrotic foci or In areas of swollen hepatocytes Mlcroveslcular change of periportal hepatocytes - slight iki It 1 focal microscopic foci of hepatocyte degeneration or necrosis with mononuclear inflammatory cell reaction - very slight orslight - moderate Periportal mononuclear Inflammatory cells - slight Bile duct hyperplasia - slight 1 0 0 0 0 0 0 0 0 2 4 0 0 0 Moribund or Dead after ^ 3-4 Exposures" 6 1000________________ _____ 3 or 5 Days Recovery 5 13 Days Recovery 6 2 21 1 11 0 02 0 01 6 00 4 00 2 00 0 20 1 21 0 23 0 23 0 10 0 01 0 01 Data presented are number of animals having the stated obaervatlon. aCraupcd by days after final (fourth) exposure when necropaled. ** Includes 3 dead after three expoeures and 1 dead and 2 moribund after four exposures. ___________ 3000 3 or 3 Days Recovery 5 13 Days Recovery 6 32 00 11 10 00 10 00 20 12 02 32 11 01 00 00 137998 C O N F ID E N T T TABLE 7 (Continued) VINYL CHLORIDE: RESULTS OP TWO SHORT-TERM INHALATION STUDIES Histopathologic Observations - Study A Mice Exposure Level (pins) Necropsy Group4 Nunber of Klee In Croup Callbladder (nunber exanlned nlcroacoplcally) No visible lesions Subaucosal edesa - slight Subuncosal inflannatory cells, nixed, focal - very slight Kidney (nunber exanlned nlcroacoplcally) No visible lesions Increased cytoplasnlc granularity, cortical tubules - very slight to slight Hlcrovesiculutlon, cortical tubules - slight Tubular eoslnophllla or nlcrovealculatlon and nuclear pyknoala; consistent with autolytic change Tubular casts. Isolated - very slight Cortical tubular cyst. Isolated - very slight Focus of basophilic tubules - very slight Interstitial Mononuclear lnflannatory cells, focal - very slight Pelvic dilatation - slight 0 3 or 5 Days Recovery 5 4 3 0 l 3 2 0 0 0 0 0 0 1 0 Moribund or Dead after ^ 3-4 Exposures 6 6 A 0 0 6. 0 1000 3 or 5 Days Recovery 5 4 3 0 1 3 1 13 Days Recovery 6 0 - - 0 - 20 01 4 0- 01 l 1- 0 1- 0 0_ 0 0- Data presented are lumber of anlsuls having the stated observation. ^Grouped by days after final (fourtli) exposure when necropaled. ^Includes 3 dead after three exposures and l dead and 2 Moribund after four exposures. - Indicates noL applicable. 2> 5000 3 or 5 Days Recovery 5 13 Days Recovery 6 43 33 10 00 30 1- 0 00- 0 0 -* 0- 11- u T DO 1 3 7 9 9 9 C O N F ID E N T IA L TABLE 7 (Continued) VINYL CHLORIDE: RESULTS OF TWO SHORT-TERM INHALATION STUDIES Histopathologic Obaetvatlona - Study A Nice Exposure Level (pw) Necropsy Croup Nuaber of Mice in Group Thymus (nuaber exaalned microscopically) Ho visible lesions Atrophy, losa of cortical lyapliocytea, with karyorrbeclic nuclei - slight - moderate - marked Karyorrliectic nuclei In medulla - slight - moderate - marked Basophilic round bodies in cortex (ninerailzed debris) Spleen (nuaber exaalned alcroscoptcally) Ho visible lesions Lyaphold depletion - slight - aodcrate - Barked Extraaedullary heaopolesls - alight to Moderate Mesenteric Lyaph Nude (nuaber exaalned alcroscoplcally) No visible lesions Lymphoid depletion - moderate - marked 0 3 or 5 Days Recovery 5 3 3 0 0 0 0 0 0 0 0 - - 0 _ - Moribund or Dead after ^ 3-4 Exposures" 6 S 0 1000 3 or 5 Days Recovery 5 3 3 13 Days Recovery 6 0 - 1 02 02 0- 1 0_ 2 02 01 0- 6 00 0 -3 _2 -1 -- 4- 2 00 0 1 -1 -- Data presented are number of animals having the stated observation. aCrouped by days after final (fourth) exposure vlren necropsled. ^Includes 3 dead after three exposures and X dead and 2 moribund after four exposure* - Indicates not applicable. 5000 3 or 5 Days Recovery 5 13 Days Recovery 6 40 3- 010_ 0 0_ 00- 00 --- __ -" 00 _ -_ -- i I. DO 1 3 8 0 0 0 eONFTDFNTTAL TABLE 1 (Continued) VINYL CHLORIDE: RESULTS OF TWO SHORT-TERM IMIALATIOK STUDIES Histopathologic Observations - Study A Nice Ex posu re Uvel (p'p) Necropsy Group Nuaber of Nice In Croup 0 3 or S Days Recovery 5 Moribund or Dead after ^ 3- A Exposures8 6 Thoracic Lynpli Node (nunber exanlned microscopically) No visible lesions Acute subcapsular hemorrhage 0 - 1 0 1 tleurt (number exanlned microscopically) No visible lesions Isolated focal areas of minute granular basophilia (mineralization) of uyocyte aytoplasn, microscopic - very slight 0 - s 2 3 Lungs (number examined microscopically) No visible lesions Edeu - very slight to slight - perivascular and peribronchial - alveolar Alveolar macrophages, increased - very slight 0 - _ - - 6 0 3 1 2 Cecum (nuaber examined microscopically) 'No visible lesions Subaucosal edema, diffuse - noderate Depletion of cells of'lamina propria . - very slight 0 _ - 1 0 1 1 Colon (nusdter examined microscopically) No visible lesions Subaucosal edena - slight 0 - 6 5 1 Data presented are ^number of actuals having the stated observation. "Grouped by days after final (fourth) exposure when oecropeled. ^Includes 3 dead after three exposures and 1 dead and 2 moribund after four exposures. - Indicates not applicable. 1000 3 or 5 Days Recovery 5 0 0 - . 0 - 0 _ 0 - 13 Days Recovery 6 0 0 - . 0 _ -- 0 _ 0 - i 3000 3 or S Days Recovery 5 13 Days Recovery 6 0 0. "-" 00 -- _ 00 --- -00 -_ -00 --- DO 138001. C O N F T D F N T IA L TABLE 7 (Continued) VINYL CHLORIDE: RESULTS 01' TWO SHORT-TERM INHALATION STUDIES Histopathologic Observations - Study A Mice Exposure Lcve I ([>pm)_____________________________ ______ 0 3 or 5 Days Hecropsy GroupRecovery Humber of Hlce In Croup_________________________ _______________ S Moribund or Dead after ^ 3-4 Exposures 6 Pancreas (number examined nlcroscoplcally) Ho visible lesions Increased lobular separation, suggestive of edema 0 6 5 1 Testes (number examined microscopically) Ho visible lesions Scattered spermatid giant cells 0 6 A 2 Epididymis (number examined microscopically) Ho visible lesions Focal necrosis of mucosa - very slighL Decreased spermatozoa In luaen 0 - - 6 4 1 1 For the following tissues, no visible lesions (number examined microscopically): Aorta Brain Coagulating Cland Esophagus Larynx Parathyroid Cland Peripheral Nerve (sciatic) Pituitary Cland Prostate Salivary Clauds Seminal Veaicles Skeletal Muscle Small Intestine Stomach Thyroid Glands Trachea Urinary Bladder 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 6 3 5 6 2 4 4 6 6 5 1 6 6 6 4 5 Data presented are number of animals having the stated observation. aCrouped by days after final (fourth) exposure when necropsled. b Includes 3 dead after three exposures and 1 dead and 2 moribund after four exposures. - Indicates not applicable. 10(10 3 or S Days Recovery s 0 - 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13 Days Recovery 6 0 - 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 t 5000 3 or 5 Days Recovery S 13 Days Recovery 6 00 - 00 00 I 00 00 o0 00 00 0o 00 00 00 00 00 00 00 00 00 00 00 -40- TABLE 8 VINYL CHLORIDE: RESULTS OF TWO SHORT-TERM INHALATION STUDIES Histopathologic Observations - Hamsters Exposure Level (ppm) Liver (number examined microscopically) No visible lesions Hepatocyte mlcroveslcular change, centrl- lobular and mldzonal - very slight to slight Multifocal microscopic aggregates of mono nuclear and reticuloendothelial cells - very slight Isolated small focus of subcapsular coagulation necrosis with mixed inflammatory cell reaction Periportal inflammatory cell accumulation, mixed type - slight - moderate Hemosiderin-laden macrophages, periportal - slight Gallbladder (number examined microscopically) No visible lesions Submucosal mononuclear cells, primarily lymphoid, focal - slight diffuse - very slight Adventitial mononuclear cells - slight Kidneys (number examined microscopically) No visible lesions Dilatation of multiple cortical tubules and epithelial vacuolization - very slight Eosinophilic hyaline casts - very slight Thymus (number examined microscopically) No visible lesions 0 1000 5000 3 0 2 1 0 2 1 1 1 0 0 0 1 3 3 0 0 3 3 Data presented is number of hamsters with the stated observation. hamsters necropsied 5 days after fourth consecutive exposure to vinyl chloride. DO 13800? CONFTDFNTTAl -41- TABLE 9 VINYL CHLORIDE: RESULTS OF TWO SHORT-TERM INHALATION STUDIES Histopathologic Observations - Rats3 Exposure Level (ppm) Liver (number examined microscopically) No visible lesions Microscopic mononuclear and reticulo endothelial cell aggregates, multifocal, occasionally adjacent to isolated degenerated hepatocytes - slight Periportal Inflammatory cells, mixed type, - slight - moderate Kidney (number examinedmicroscopically) No visible lesions Scattered tubules with eosinophilic casts - slight Cortical interstitial mononuclear cell accumulation, focal - veryslight Focus of fibrosis and chronic inflammation - slight Focus of mononuclear cells, primarily lymphoid, beneath pelvic epithelium - very slight Isolated cortical focus of hypertrophied, basophilic tubules - very slight Thymus (number examined microscopically) No visible lesions Petechial hemorrhage of medulla - very slight Anterior Mediastinal Lymph Node (number examined microscopically) Lymphoid hyperplasia and sinusoidal hemorrhage 0 3 0 1 2 1 3 0 1 2 1 0 1 3 3 0 0 1000 3 0 3 3 0 3 1 1 1 0 1 0 3 2 1 1 1 Data presented is number of rats with stated observation. ^ats were necropsled 5 days after the fourth consecutive exposure to vinyl chloride. - Indicates not applicable. DO 138003 CONFIDENTIAL -42- TABLI 10 Vim CHLORIDE; RESULTS OF TWO SHORT-TERM INHALATION STUDIES Hl*cop*thologic Qbeervationa Study B Mica Expoaura Laval (pw) Hecropev Croup Nunbar of Mica In Croup 0_______ Final Termination 5 Liver (number examined mlcroecoplcally) No vialbla laaiona Acuta coagulation necroela, - focal, cantrllobular, about 10Z of araa axanlnad. oftan eubcapaular - focal, centrilobular to confluant, involving 25Z of araa of aactiona 5 1 0 0 focal, cantrllobulat to confluant, involving 26-501 of area of aactiona axanlnad - focal, cantrllobular to confluant, involving 51-75% of araa of aactiona 0 0 Iaolatad focal nacrotlc araa - alight Minute, Intracellular baaophllic atructurea at oarglna of nacrotlc araaa Perlnecrotic inflammatory calla, nixed - very alight to alight Perlnecrotic tubacute lnflaimatory and connective tiaaua calla - alight Perlnecrotic avollen, vacuolated hepacocytea Cantrllobular hapatocytaa, microvaaicular change - vary alight to alight - narked Cantrllobular and nldzonal hapatocytaa enlarged, honngeneoue cytoplasm of slightly increased basophilia, occasional nitoees Focal subcapsular hepatocyte degeneration vith vacuolatlon and hyaline naterial Sinusoidal congestion and hemorrhage, in and near necrotic areas - moderate - narked Cantrllobular vein congestion, compression of adjacent hepatocytea - narked Focal telangiectasia Snail hyaline nesses in slmisoida, margins of necrotic foci or in areas of avollen hapatocytaa Multifocal microscopic foci of hepatocyte degeneration or necrosis vith mononuclear inflammatory cell reaction - very slight to slight 0 0 0 0 0 1 0 0 0 0 0 0 0 0 3 Gallbladder (number examined microscopically) No visible lesions 3 3 ___________ 1000_____________ Final 2 Exposures Termination lb 4 14 00 03 01 10 00 00 10 00 04 11 00 00 0u 01 01 10 10 03 02 00 13 13 5000 Final Termination 5 5 0 0 0 1 1 1 2 1 0 1 2 1 2 0 0 2 3 0 0 2 2 Dace presented are number of animals having che stated observation. *Necropsled after five exposures. Animal died after two exposures. D0 138004 CONFIDENTIAL -43 TAILS 10(Continued) Tim CHLORIDE: REMITS OF TWO SHORT-TERM INHALATION STUDIES Histopathologic Observations - Study I Hie* Exposure Level (ppm) Necropsy Croup Number of Mice in Group 0 Final Termination4 3 1000 Final 2 Exposures Termination lb 4 3000 Final' Termination 3 Kidney (number ersntlneri microscopically) No visible lesions Enlarged cells> scattered, inner cortical tubules - slight Mlcrovealculatlon, cortical tubules - slight to moderate Focus of basophilic tubules - very slight Interstitial mononuclear inflammatory cells, focal - very slight Medullary casts, isolated, occasionally mineralised - slight Thvnus (number examined microscopically)- No visible lesions Atrophy, loss of cortical lymphocytes, with karyorrhexie aide! - slight - marked Basophilic round bodies In cortsx (mineralised debris) Thyroglossal duct cyst, isolated, microscopic 3 3 0 0 1 1 1 3 5 0 0 0 0 1. 1 0 0 0 0 0 1 0 0 1 0 0 4 3 0 1 0 0 0 4 2 1 1 0 0 5 0 1 3 1 0 0 3 3 0 2 i i Data presents! are number of animals having th atatad observation, ^ecropsied after five exposures. bAnimal died after tun exposures. DO 138005 confidential. DISTRIBUTION ADCOCK, L.D., 2020 Dow Center AXE, P.D., Pittsburg, CA 'X BEARDEN, C.R., Granville \BRADLET, T.D., 834 Bldg. \RANSON, D.R., 1803 Bldg. BHaUN, W.H., 1803 Bldg. BRAUNLICH, F.H., Dowell, Tulsa BURGEfiT, B.E., 1603 Bldg. BURGESS. K.L., 1803 Bldg. CALDWELLVB.G., 47 Bldg. CAFUTO, r\ Sao Paulo CHEN, W.L.jNSarnia CORSON, F.P.\Coral Gables CRUMMETT, W.B.\574 Bldg. CURRIER, H.F., Ptaquemine, LA DANIEL, R.L., B-lbl, Freeport DAVIS, K.R., 2020 Dmt Center DICKSON, G., 322 BldV DOSTAL, R.L., 1803 Bid*. EILERS, R. J., Van Nuys\CA ENGIBOUS, D.L., Plaquemln^. LA FARBER, H.A., 2020 Dow Center FISHBECK, W.A., Freeport, Tj\ FLAKE, R.E., Freeport N FLORES, G.F., Freeport, TX GAT, P.C., Oyster Creek GEHRING, P.J., 2020 Dow Center GERARDO, R.A., Oyster Creek GORZINSKI, S.J., 1803 Bldg. GRAHAM, D.L., Pittsburg, CA HALPHEN, C.E., Plaquemine, LA HEFNER, R.E., 1776 Bldg. HOERGER, F.D., 2030 Dow Center HOOVER, W.E., 1803 Bldg. HYLTON, D.B., Strongsville JACKSON, J.E., Indianapolis y JERSEY, G.C., Lake Jackson / KAGEL, R.O., 2030 Dow Center/ KELLY, M.E., 2020 Dow Cente/ KLUMB, G.A., 566 Bldg. / KOCIBA, R.J., 1803 Bldg. / KOLESAR, R.C., 607 Bldg,/ LANCINI, G., Lepetit / LANGNEB, R.R., 2030 Do/ Center LANHAM, J.M., Pittsbu/g, CA LEHMAN, F.R., 2040 D/w Center LENG, M.L., 1803 Bldg. LIU, G., 566 Bldg./ LOOK, A.T., Houst/n, TX LUOMA, E.V., 202/ Dow Center LYONS, K.L., Li/king River MacGOWAN, C.F./ Washington, DC is' Entire copy of the report. MACKEY, J.C., 2020 Dow Center McCLURE, H.H., APB, Freeport McCOLLISTER, D.D., 1803 Bldg. McDANIELS, G.D., Strongsville (Eastern) McFEDRIES, Jr., R., 2040 Dow Center McKENNA, M.J., 1803 Bldg/ MOOLENAAR, R.J., 1702 Bldg. NEWBY, H.E., Houston, Kt NICHOLS, J.L., Hong Kong NORRIS, J.M., 1803 Bldg. NOWAK, R.M., 2040 Dow Center NUMMY, W.R., 2020/Dow Center OLSON, K.J., 180/3 Bldg. OLSON, R.D., Sarnia OUBRY, R.R.,. Oyster Creek PARENTI, F.,/Indianapolis PUMPELLY, C/T., Dowell, Tulsa RAMPY, L.W/, 1803 Bldg. RAO, K.Sy/ 1803 Bldg. RAUSCH,/.A., 2020 Dow Center REUVERS; J. H., Terneuzen RINZEMA, L.C., Horgen ROGER^, W.A., 2020 Dow Center ROZAS, E.A., Strongsville SAUNDERS, J.H., 607 Bldg. SCHAFFER, A.W., Hong Kong SCHARNWEBER, H.C., 2030 Dow Center /SCHNEIDER, E.J., 1803 Bldg. XteCHWETZ, B.A., 1803 Bldg. SHEETZ, D.P., 2020 Dow Center SHELTON, L.G., Oyster Creek SKOBY, L.K., 2020 Dow Center STOTTV W.T., 1803 Bldg. SWANK^Nm.G., 1803 Bldg. TALCOTt\a.T., 2030 Dow Center THOMKA, IAM., 2040 Dow Center THOMPSON, BLF., Indianapolis TOD, L.M., Sarnia TORKELSON, t\., 1803 Bldg. VENABLE, J.R.,\l803 Bldg. VERSCHUUREN, H.O-, Rotterdam VRANISH, S.R., 9008 Bldg. WARNER, S.D., Indianapolis WATANABE, P.G., l80\Bldg. WESELOH, J.W., 9008 Bldg. WRIGHT, L.F., APB, Fr^port WROBLEWSKI, D.J., l803%dg. YOCUM, R.H., 566 Bldg. \ YOUNG, D.M., Sarnia \ CRI, 566 Bldg. (4) \ TOX FILES, 1803 Bldg. (4) \ DO 138006 CONFTDFNTIAL,