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Acute PFOS ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.,DAPHNIA) TEST SUBSTANCE Identity:Perfluorooctanesulfonatmea;y alsobe referredtoas PFOS or FC-95 oras partofthe mixed productFM-3820 (see Remarks). (1-Octanesulfoniaccid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium salt,CAS # 2795-39-3) Remarks: The testsample isFM-3820, a mixtureofthe testsubstance in diethyleneglycolbutyletherand water (approximatel2y4-28% test substance indiethyleneglycolbutyletherand water).Calculationwsere made toadjusttestvaluesusingtheupper limictoncentratioonfthe test substance (28%) inthetestsample and no adjustmentwas made forthe presence ofthediethyleneglycolbutyletherorwaterwhen noted below. These calculationasssumed thatalltoxicitwyas due tothe presence of the Perfluorooctanesulfonastuebstance. METHOD Method: OECD 202 Test type: Staticacute GLP: Yes Year Completed: 1991 Species:Daphnia magna Analyticalmonitoring:DO, pH, Conductivitayn,d temperaturewere monitoreddaily. Statisticamlethods: EC5o valuescalculatedw,hen possibleby standard statistictaelchniques(Stephan,1983) Test daphnid source: Obtainedfrom culturesmaintainedby EnviroSystems DivisionR,esource Analysts,Inc.,Hampton, NH. Test daphnid age atstudy initiation<:24-hours Test conditions Dilutionwater:Wellwaterfrom wellsat EnviroSystemsin Hampton, New Hampshire. Dilutionwater chemistry: pH: 7.8* Conductivity: 1200 umhos/cm* TOC: <2.0 mg/L Values measured attimeoftest. Lighting:Cool whitefluorescentlightsi,ntensit2y3 uE/s/M2. Photope@od of 16-hourslight8,-hoursdark. No transitiopneriod noted. Stockand testsolutionpsreparatioAn:primarsytocksolution was preparedindilutiownaterat 1000 mg/L. The primarystock was proportionaldliylutedwithdilutiownater topreparethe fivetest concentrations. Exposure vessels:250 mL plastibceakers r.,ontaini2n0g0 mL of testsolution.The approximatedepth oftestsolutionwas 6 cm. Number of replicatesF:our Number of daphnids per replicateF:ive Number of concentrations:fiveplusa negativecontrol Water chemistry during the study: Dissolved oxygen range (0- 48 hours): 8.2- 8.5mg/L (controelxposure) 8.1- 8.5mg/L (150mg/L exposure) pH range (0- 48 hours) 7.8- 8.6 (controelxposure) 7.8- 8.6 (150mg/L exposure) Test temperature range (0- 48 hours) 20.8- 21.0"C (controelxposure) 20.7- 20.90C (150mg/L exposure) Conductivityrange (0- 48 hours) 1200 - 1300 umhos/cm (controelxposure) 1200 - 1300 umhos/cm (150 mg/L exposure) Element basis:mortality RESULTS Nominal concentrations: Bk control2,5, 40, 60, 100, 150 mg/L Element values: 24-hour EC5o = >150 mg/L (C.I.not calculable) 48-hourECro = 49 (43-56)mg/L Perfluorooctanesulfonateconcentrationadjusted Element value: 24-hourEC50= >42 mg/L 48-hourEC50 = 14 mg/L Allelement valuesbased on nominal concentrations Biologicalobservations:Ninetyfivepercentsurvivaolccurredinthe controlexposure.The number ofsurvivingorganisms and the occurrence ofsublethaleffectsand immobilizatioonrothersublethaleffectswere determinedvisuallaynd recordedinitialalnyd after24 and 48 hours. Cumulativepercentm rtality: NominaTlest -Conc.,mg/L 24-hours 48-hours Neg. Control 0 5 25 0 0 40 5 25 60 0 25 100 0 100 150 20 100 Control response: satisfactory CONCLUSIONS The testsubstance 48-hour ECro forDaphnia magna was determined to be 49 mg/L witha 95% confidenceintervaolf 43-56 mg/L. Ifyou assume alltoxicitoyf the mixtureisdue tothe Perfluorooctanesulfonatteh,e adjusted48-hour EC5o value is14 mg/L (49 mg/L X 0.28). Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY Reliability:Klimischranking= 2. The study lacksanalytical measurement oftestsubstance concentrationsinthe testsolutionsand sample purityisnot sufficientclhyaracterized.Additionallyd,ata isfora mixtureand toxicitcyannot be positivelayttributetdo Perfluorooctancesulfonataes the diethyleneglycolbutylethercouldalso contributeto the toxicityT.he basicwater qualityparameters (hardness, alkalinitaynd calcium/magnesium ratiow)ere not includedinthe final report. REFERENCES This study was conducted at EnviroSystems DivisionR,esource Analysts, Incorporated,Hampton, NH atthe requestofthe 3M Company. OTHER Last changed: 5/3/00 08/19/1991 ENIIR34ERD%" LABA@ATM FNAL REPCW," 1 LAB FSQUEST M. H2864 REUMQTM N*E: EA FENZPJSAB DUARn4D-T: 0535 PROJECR NO: 5918340000 DATE FEMIVED: 11/09/1990 DESC: FC-203 COMPCNarrS SAMPLF- DATE OME DEScRIPriaN camwr LAB: EST CMin4A= IAB COST: $12,000.00 --1E4-L HCM: 0 E(P CCFP DAME: 03/05/1991 DA77 OCF@PT 08/19/1991 F4@S= ML CR (C.I.) 1 F4M 3017 *DAPM'IA ACUTE 2 E44 8887 'DAPh-nA A= 3 R4 3021 *DAPMIA ACUTE 034PT 4 R4 27513 -DAPHNIA ACUTE T= 5 FM 4115 *DAPMIA ACUTE = COWT 6 FM 3820 *DAPPNIA ACUTE = OCFEI= 7 R4 55040 *DAPHNLA ACUTE TEST CCHPTP-Ill-o 8 Fo 199 *DAPHNIA ACUTE 7= ccwl= 9 FC 203 *DAPMIA AcL?rE CCWT 10 VW 160390 *DAPEt4IA ACUTE OWT CCZvrPJkCT IAB SUEM= BY: DATM: Study Title Static Acute Toxicity of FM 3820 to the DapbLnid, Daphnia sagna Authors Timothy J. Ward Robert L. Boeri Study Completed March 26, 1991 Performing Laboratory Envirosysteas Division Resource Analysts, Incorporated P.O. Box 2130 One Lafayette load Hampton, New Hampshire 03842 EnviroSystess Study Number 90171-3 Page I of 17 PemkomAtctvfrtc &ANOM d MUFORE 1. GWD LABORILTOITYMCTICZ STZTMMff This study was conducted according to OECD C-ood Laboratory Practice Regulations.Neither the Study Director nor the sponsor are aware of any circumstancesthat would affect the integrityof this study. 12 t, . T thy 4. Wir-d bor and Study Director Robert L. Boeri Coauthor 3-27 q -Jeame P. maga(zu@. AquaticToxi-logist PetVerL. Kowalski Aquatic Toxicologist gllen J bdtanford Aquatic Toxicologist KaviroSysteas Study Number 90171-3 Page 2 of 17 RowtocAeox*vft 5rr-&jbgdKNV d K*IFM 11. QUALM JLSSUIAM SnTzxm Submitted by: EnviroSysteas Division Resource Analysts, Incorporated P-0. Box 2130 One Lafayette Road HaRPtOD, New Hampshire 03842 Certification: Data presented in this report were derived by methods and with materials identified in the section of the report entitled "methods and Materials." The test was Performed in accordance with ZnviroSystess Protocol 90171-3 and the Product Registration Aquatic Toxicology Laboratory Standard Operating Procedures Xanual. The toxicity test was performed by Ellen Stanford, Jeanne Magazu, Peter Kowalski, Robert Roeri, and Timothy Ward. The original raw data and final report will be archived at Resource Analysts, Inc. for at least 10 years. All data transcribed from the raw data to the report were checked for accuracy, and all data were verified by Quality Assurance Auditors. Quality assurance audits were performed on Audit Date 11/12/90 12/20/90 2/ 9/91 3/28/91 Reported to Study Director 11/14/90 12/20/90 2/ 8/91 3/23/91 Reported to Management 3/28/91 3/29/91 3/23/91 3/28/91 Qu&litt Assurance Representative EnviroSystea3 study Number 90171-3 Page 3 of 17 Re".Oce Ar<gyft WC- &JbgdooryofKMUJPCK M. TIM OF cowrzwrs SECTION: I. Good Laboratory Practice Statement Il. Quality Assurance Statement III. Table of Contents IV. Index of Tables V. Summary VI. Introduction VII. Metbods and Materials VIII. Results IX. References Appendix A. Water Quality Data from Toxicity Test Page 2 3 4 s 6 7 7 11 14 EnvirOSy3tems Study Number 90171-3 Page 4 of 17 RewkK* Aix3ivftkr- Sibuckcryd K*IFORE IT. INDEX OT TILBLZS TahLle 1. Ta4ble 2. Cbenical cbaracterization of a representative sample of natural well water used as dilution water for toxicity test Survival data from toxicity test Tabble 3. Median effective concentrations (EC50s) from toxicity test Tabble A.I. Conductivity, VB, temperature, and dissolved oxygen concentration measured during toxicity test Page 12 13 16 ErL,aiiroSysteasStudy Number 90171-3 Pagige 5 of 17 ResoirA-rec*vfktv-SjbgckmdyK*IVORE T. suxxmry The acute toxicity of FN 3820 to the dapbuid, Daphnia magna, is described in thisfinalreport. The test was conducted for 3M Cospany for 49 hours during December 18 to 20, 1990, at the EnviroSystems Division of Resource Analysts, Inc. in Hampton, New Hampshire. It was conducted by Illen Stanford. Jeanne Magazu, Peter Kowalski, Robert Doeri, and Tinotby ward according to the protocol developed for EnviroSysteas Study Number 90171-3. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 20 t IOC. The dilution water was filtered natural well water collected from wells at Hampton, New Hampshire. Aeration was not esployed to maintain dissolved oxygen concentrations above an acceptable level. Nominal concentrations of test substance were: 0 mg/L (control), 25 mg/L, 40 mg/L. 60 mg/L, 100 ug/L, and 150 mg/L. Nominal concentrations were used for all calculations. Dapbnids used in the test were produced fro.%an in-house culture and were less than 24 hours old at the start of the test. After 48 hours of exposure the control daphnids had an average wet weight (blotted dry) of 0.0001 g, resulting in a loading rate of 0.002 g/L. All daphnids were in good condition at the beginning of the study. Rxposure of daphnids to the test substance resulted in a 48 hour median effective concentration (EC50) of 49 mg/L TM 3820, with a 95% confidence interval of 43 - 56 mg/L. EnviroSystems Study Number 90171-3 Page 6 of 17 QOWLGCO k4WL M. &"K" Ot MLLPM TI. irmwctlcw Thisstudywas sponsoredby 3H Company,St. Paul,Minnesota- The objective Of the study wag-to.daterainethe acute toxicity of FN 3820 to the daphnid, a freshwaterinvertebrate. The report contains sections that describe the methods and materials employedin the study,and the results of the investigation. The reportalso containsan appendix that presentsthe water quality data collected during the'test. TEST SUBSTANCE: Vil. KCMDS MM KRTZLLLLS FM 3820 (ZnviroSysteas Sample Number 26961) was delivered to EnviroSystess on Noveaber 15, 1990. It was containedin a 250 &L plastic bottle that was labelledwith the followinginformation:-3 2864-6, rN 38201. The test substance was supplied by 3N Company, St. Paul, Minnesota. Prior to use it was stored in the dark at room temperature. A reserve sample will be arcblivedat EnviroSystess for a minimum of 10 years. DRLUTION WATER: Water used for acclimationof test organismsand for all toxicity testing was collected from wells at EnviroSysteas in lampton, New Nampshire. Water was stored in 500-gallonpolyethylenetanks where it was aerated.Results of chemical analysis of a repre30UtatiVesample of water are presented in Table 1. TEST ORGANISM: Juvenile daphnids-exployedas test organisms were from a single source and were identifiedusing an appropriatetaxonomickey. Daphaids used in the test were produced from an in-house culture and were less than 24 hours old at the startof the test. Control dapblnidswere weighed at the conclusion of the toxicitytest. Prior to testing,daphnidswere maintainedin 100% dilutionwater under staticconditionsin 4 liter glass jars. During the 14 day acclimation daphnid cultures were not treated for disease and they were free of apparent sickness,injuries,and abnormalitiesat the beginningof the test. During the acclimationperiod24 hours prior to the test initiationthe temperaturewas 19.0 - 19.8*C. Daphnids were fed yeast/trout chow and/or the freshwater alga Selenastruscapric rnutus daily before tbc test. govirosysteas Study Number 90171-3 Page 7 of 17 RwmoceAN*ft Wc.&"diaryd MUFM Table 1: Cbesical characterization of a representative sample of natural *ell water used-as dilaticw water for toxicity test Parameter Unit of Reasurement Reporting idnit Neasured Value PH Conductivity PR units mabos/cm Organocblorine ug/L 2 pesticides Organopbospborus ug/L S.0 pesticides Polycblorinated ug/L 0.6 biphenyls 7.9 1200 ND ND ND Ndtdig: 1'. ND - Not detected above the reporting limit. 2. The pff and conductivity values were detesined in a sample of dilution water collected from a control test vessel at the begilnnirg of the toxicity test. Additional cbenical parameters were measured in dilution water during October, 1990 as part of routine biannual water quality analysis. EnviroSysteas Page 8 of 17 Study Nuaber 90171-3 RowLsceAjw*ft bv- SimpLUoyd NOIFORE TOXICITY TESTING: A screening test with the test substance was conducted during December 5 to 7, 1990. Nominal concentrations of test substance were: 0.1, 1.0, 10, 100, and 1,000 mg/L. After 49 hours of exposure there was 0% survival at 1,000 mg/L,'60% survival at 100 mg/L, and 90% or greater survival at all other concentrations. The definitive toxicity test was performed during Deceidwr 18 to 20,.1990, according to rnviroSy3tess Test Protocol 90171-3 lstatic, Acute Toxicity Test With FM 3920 And The Daphaid, Daybuia sacrna , which was signed by the Study Director on November 16, 1990. It is based on procedures of the OECD (1984). The test was conducted at a target temperature of 20 10C with five concentrations of test substance and a dilution water control. A 1,000 mg/L stock solution was prepared by combining 1.0 9 of test substance with dilution water and adjusting the final concentration to 1,000 al without the use of a solvent. Nominal concentrations of the test material were: 0 mg/L (control), 25 ag/L, 40 mg/L, 60 *g/L, 100 mg/L, and 150 mg/L FM 3920. Twenty daphnids were randomly and equally distributed among four replicates of each treatuent. The test was performed in 250 al glass beakers that contained 200 al of test solution (water depth was approximately 6 ca). Test vessel5 were randomly arranged in an incubator during the 48 hour test (a random numbers table was used to select the location of each vessel). A 16 hour light an4 8 hour dark photoperiod was automatically maintained with cool-vbite fluorescent lights that provided a light intensity of 23 uSs'la". Aeration was not required to maintain dissolved oxygen concentrations above acceptable levels. Dapbnids were not fed during the test. The number of surviving organisms and the occurrence of sublethal effects and imobilization (inabilityto swim within 15 seconds after gentle agitation) or other sublethal effects (loss of equilibrium, erratic swimming, loss of reflex, excitability, discoloration, or change in behavior) were determined visually and recorded initially and after 24 and 49 hours. Dead test organisms were removed when first observed. Dissolved oxygen (YST Model 57 meter; instrument number PRL-3), pil (Beckman model pffl 12 utter; instrument number PRL-4), :Onductivity (Labcoup SCT meter, instrument number PRL-9), and temperature (ASTH mercury thermometer) were measured and recorded daily in each test chamber that contained live animals. EnviroSysteas Study Number 90171-3 Page 9 of 17 Qmwce NK$Yft Vv- Sjbw" d MUPORE STATISTICA]6 EMODS: Resultorof- the toxicity test were interpreted by standard statistical tecbniques (Stepban,1983). The nominal concentrationof test substance and the number of organism killed or immobilizedwere used by the autbor to calculate the EC50 values. EnviroSystess Study Number 90171-3 Page 10 of 17 kwk=o NK*ft kv- Sub@ d MUFM Tin. RUMTS All test vessels appeared clear initially and remained free of insoluble material throughout the test. Biological and water quality data generated by the acute toxicity test are presented in Table 2 and Appendix A. respectively. Ninety five percent survival occurred in the control exposure. Control dapbnids bad an average wet voight (blotted dry) of 0.0001 g at the end of the test. Loading rate during the toxicity test was approximately 0.0025 g/L. Exposure of daphnids to the reference toxicant sodium dodecyl sulfate resulted in a 49 hour median lethal concentration (LCSO) of 15.6 ag/L. The 24 and 48 hour SCS09 for daphnids exposed to TN 3820 are presented in Table 3. Exposure of dapblnids to the test substance resulted in a 48 hour ECSO of 49 mg/L FM 3820, with a 95% confidence interval of 43 - 56 mg/L. EnviroSystess Study Number 90171-3 Page 11 of 17 Resmoce Ano"L m. 9ibudKiV d MLUPORE Table 2. Survival data from toxicitytest Nominal Concentration (mg/L) rep. Number Alive 0 hr 24 hr 43 hr Number If fectea ohr 24hr 48hr 0 (control) 1 2 3 4 25 1 2 3 4 40 1 2 3 4 60 1 2 3 4 100 1 2 3 4 250 1 2 3 4 5 5 4 5 5 5 5 5 s 5 5 5 5 s s 5 5 5 s s 5 5 5 5 s s 3 5 s s s s 3 5 4 4 5 5 2 5 5 4 s 5 4 5 5 5 5 s 0 s 5 0 5 5 0 5 5 0 s 3 0 s 5 0 5 4 0 5 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 4 0 0 3 0 0 - 0 0 0 0 0 0 0 0 0 1 0 0 0 0 Note: Affected daphnids were iambilized (unable to swim within 15 seconds after gentle agitation) InVirOSYSteM3 Study Number 90171-3 Page 12 of 17 ResoLoceAnoWL kic.&.jbpdcrvd K*LFOK Table 3. Median effective concentrations (EC50s) from toxicity test Exposure period 9S percent Calculation EC50 confidence limits method 24 hours 49 hours )lSO mg/L 49 mg/L 43 S6 ag/L Probit EnviroSyst*as Study Number 90171-3 Page 13 of 17 Resot"kn@^ ky-&ft*M d MUFM IX. RErztERM OECD. 1981. Decision of the Council Concerning the Mutual Acceptance of Data in the Assessment of Chemicals. Innex 2. OECD Principles of Good Laboratory Practice. C (81) 30 (rinal). OECD. 1924. Guidelines for Testing of Chemicals. Section 2: Effects on Biotic Systems. Method 202, Daphnid sp., Acute l@obilization Test and Reproduction Test. Adopted April 4, 1984. Stephan, C.E. 1983. Computer program for calculation of LCSO values. Personal communication. EnviroSystems Study Number 90171-3 Page 14 of 17 Re=Lom Arayft We- &IxKkyy d kliPM Appendix A. VATER OUALTTY DATA TROL TOXICTTY TSST EnviroSystess Study Number 90171-3 Page 15 of 17 PSMLNCOAJX*ftby-91=*W d K%UVK Table A.I. Conductivity, pl, temperature, and dissolved oxygen concentration measured during toxicity test Nominal concentration (mg/L) Rep. 0 (control) 1 2 3 4 25 1 2 3 4 40 1 2 3 4 60 1 2 3 4 100 1 2 3 4 150 1 2 3 4 Conductivity (UWW/Cn) 0 24 48 hr hr hr 2200 1200 1200 1200 1200 2200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1200 1300 1300 1300 2300 1300 1300 1300 1300 1300 1300 1300 1300 1300 2300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 1300 Pil 0 24 48 hr hr hr 7.8 3.2 8.6 7.8 8.2 8.6 7.8 8.2 9.6 7.8 8.2 9.6 7.8 8.2 8.6 7-8 8.2 3.6 7.8 8.2 8.6 7.8 8.2 8.6 7.8 8.2 8.6 7.8 9.2 8.6 7.8 8.2 8.6 7.8 8.2 9.6 7.8 8.2 8.6 7.3 8.2 8.6 7.8 8.2 8.6 7.8 8.2 9.6 7.2 3.2 8.6 7.8 8.2 9.6 7.8 8.2 8.6 7.8 9.2 8.6 7.8 9.3 9.6 7.8 8.3 8.6 7.9 8.3 8.6 7.8 8.3 8.6 EnviroSystems Study Number 90171-3 Page 16 of 17 ReouimAnoWt W- Sibma id ktiFORE Table A.l. Continued. I Nominal concentration (mg/L) Rep. 0 (control) 1 2 3 4 25 1 2 3 4 40 1 2 3 4 60 1 2 3 4 100 1 2 3 4 150 1 2 3 4 Temperature (OC) 0 24 49 hr hr hr 20.0 20.8 20.9 20.9 20.8 20.9 20.7 20.7 20.9 20.8 20.9 20.9 20.7 20.7 20.9 20.9 20.8 20.7 20.8 20.8 20.9 20.7 20.8 20.8 20.9 21.0 20-9 20.9 20.8 20.9 20.9 20.9 20.9 20.8 20.7 20.9 20-8 20.9 20.9 20.9 20.9 20.8 20.8 20.9 20.8 20.9 20.9 20.9 21.0 21.0 20.9 20.9 20.9 21.0 21.0 21.0 20.8 20.6 20.6 20.9 20.9 20.9 20.9 20.9 22.0 20.9 20.9 21.0 20.9 20.9 20.9 20.9 DirsolvedOzrgen (ag/L) 0 24 48 hr hr hr 8.3 8.2 8.5 8.3 3.2 8.5 9.3 8.3 8.5 8.3 8.2 9.5 9.2 8.2 8.5 8.3 3.2 3.5 8.3 3.1 8.5 8-3 3.1 9.5 8.2 8.2 8.4 8.2 3.2 9.3 8.3 8.3 8.5 8.3 3.2 3.5 8.3 9.1 8.0 8.2 8.1 8.3 8.2 9.2 8.3 8.3 8.3 9.4 8.2 8.1 8.5 9.3 8.1 9.4 8.2 3.3 8.3 9.2 8.2 8.4 8.3 3.1 S.S 8.2 8.2 3.5 8.2 3.2 8.4 8.2 8.2 8.5 EnviroSysteas Study Number 90171-3 Page 17 of 17 ftwume Nv*ft kv- S@tK" ofMt6-,QE Envir*Systeas Study Number 90271-3 RESOURCE ANALYSTS, INCORPORATED MRMOSYSTERS Drvrsiow PRODUCT RMSTRJKTICK LABORATORY IkQtMTICTmacoLocT STUDY PROTOCOL Static Acute Toxicity Test With FN 3820 And The Daphaid, Daphnia Alagn& SL)onsor 3N Company PO Box 33428 St. Paul, Kinnesota 55133 Sponsor Approval of Protocol Date Testina Facility InviroSysteas Division Resource Analysts, Inc. I Lafayette Road Nampton, WH 03842 Estimated Test Initiation Date: October, 1990 Estimated Test Completion Date: Decenber, 1990 Study Director: Timothy J. Vard Study Director Approval of Protocol Q040r@ate -1,Llotf 0 Page 1 of 6 THIS ISA CERTIMED TRUE COPY. SIMATURE r-M DATE--S-/-,,l-i-i PO/2#A ROCUVOPjx*ftb--SL-mcFadyLOIFM EnviroSystess Study Number 90171-3 1.0 Tint Static. kcute.-Taxicity-Test Vitb FX 3820 Ind The Daphnid. Dephnia Magi?& 2.0 PURPOSE To determine the 24 hour and 48 boar median effective concentrations (EC50s) of the test substance to the dapbaid, when exposed for 43 boors under static conditions. 3.0 TEST XATERTAL 3.1 The te3ti substance and related stability and purity data will be supplied by the sponsor. Test material will be stared at room temperature in the original shipping container unless alternate storage conditions are requested by the sponsor. Bandling of the test material will be in accordance with information contained in the sponsor-suppliedNaterial Safety Data Sheet and RAI Standard Operating Procedure 01-02-1106. A subsample of test substance (approximately 1 gram) will be removed from the original container and transferredto a glass vial for archiving. kll unused test substance will be returned to the sponsor. 3.2 Calculations are based on nominal concentrations of the test substance. Test substance stock solutions are prepared in deionized or dilution water without the use of a solvent (carrier)if possible. If a solvent is required, dinetbylformaside, trietbylene glycol. or acetone will be used. The concentration of solvent will be limited to IDO mg/L- 4.0 TEST SPECIES 4.1 The selection of test species is determined by the sponsor. Healthy juveniles (free from known diseases) from a single source will be used to initiate the test. They will be obtained from an in-house culture with a known history and will be less than 24 hours old at the initiation of the test. The wet weight of control organisms will be determined at the end of the test. 4.2 Identification of the appropriate taxonomic keys. test animals sball be verified using 5.0 PRETEST OBSNVATIONS AND PROCEDURES 5.1 Protest observation data concerning the source, handling procedures, receipt date, disease treatment (if any), health. feeding,and mortality of test animals will be recorded and reported. Page 2 of 6 A"*ft by- d NWIJIOM ftvirosystess Study Number 90171-3 5.2 Daphnids will be maintained under static or replacementconditions prior to test initiationin the same water and at the same pbotoperiodand temperature that will be used for testing. The animalswill be fed at least once- daily with a mixture of yeast, trout chow, and ceraybyl and/or freshwater algae. Daphnid cultures will be maintainedin dilation water at test conditions (temperature,Lirdness,and photoperiod)for at least 14 days prior to testing. 6.0 EXPOSURE CONDITIONS 6.1 The test will be conducted under static conditions.and test media will be suppliedonly at the beginningof the test. 6.2 Dilution water will be natural ground water. It wil]Lbe free of measurable concentrations of pesticides. listerwill be passed througha (20 nicron filter and chemically characterized (atleasttwiceyearly analysisat Rkl) prior to use. 6.3 Tater temperaturewill be 20 :t1*C. 6.4 Dissolved oxygen concentration should be above 70% saturation,but aerationwill not be utilized. 6.5 Pbotoperiod will be automaticallycontrolled and adjusted to 16 hours light and 8 hours dark. 6.6 The test vessels will be 250 al beakers of that contain200 al of test media. The loadingrate will be less than 0.5 g/L. 7.0 STUDY CONDUCT 7.1 The results of a range findinginvestigationand/orprevious testing data will be used to select the concentrationrange for the definitive test. If a range finding test is conductedaninalswill be exposed to a series of concentrations of test substanceplus a controland a solvent control(ifrequired). 7.2 During the definitive-test the animals will be exposedfor 48 hours to at least five concentrationsof test substanceplus a control (dilution water without test substance)and a solvent control (if necessary)under static conditions. Test concentrations will be chosen to bracket the anticipated EC50. 7.3 At least 20 animals will be exposed to each treatment. The animals will be exposed in groups of 5 animals per vessel (4 replicatesper concentration),each containing 200 al of solution. The animals will be assigned to randomly positioned test vessels (a random numbers table will be used to choose the positionof each test vessel),and they will not be fed. 7.4 Animals will be added to test media after test substancehas been added and aeration will not be employed. Page 3 of 6 Raimm Arg*ft kv- Su'sdM d K%LFM EnviraSysteas Study Number 90171-3 7.5 The highest tested concentration will be 1,000 mg/L. 7.6 A taxtettr..t.--t- shall also be conducted with a reference toxicant and the batch of daphulds used for the test. 7.7 Dissolved oxygen concentration, pff, temperature, and conductivity will be measured and recorded at the beginning of the test and daily thereafter in all test vessels, as long as living animalsare present in those vessels. 7.8 Mortality and sublethal effects (immobilization)will be recorded at 24 hour intervals during the test. Dead animals will be removed. 7.9 Data Analysis and Statistical Methods 7.9.1 The NCSO values and their 95% confidence intervals will be calculated using mortality and/or immobilization data by the binomial, moving average or probit methods. 8.0 QUALITY ASSURANCE AND QUALITY CONTROL 8.1 The test will be repeated if any of the following conditions occur: 8.1.1 Control survival at the end of the test is less than 90%, or more than 10% of the control dapbnids are stressed or immobilized, or any contror animals are trapped at the surface. 8.1.2 Tesperatare in any test vessel is outside of the range of 19 - 21*C. 8.2 The test will be conducted according to OECD Good.Laboratory Practice Standards. 8.3 The test will be audited by the Quality Assurance Unit. 8.4 The Study Director will be responsible for reviewing all data and for recording any changes to procedures outlined in this protocol in a protocol amendment. Protocol amendments will be approved by the slxmsor. 8.5 Original data will be arcbived at Resource Analysts Inc. for at least 10 years or it will be transferred to the sponsor for arcbiving. Page 4 of 6 Re=xm M*ft kw-&Zmdbr d UMPM EnviroSystess Study Number 90171-3 9.0' REPORT 9.1 An originalreport will be preparedafter review of a draft report by tbw..sponsor. -The final report will be signed by the Study Director and staff that condnetedthe study and prepared the report, and by the Quality Assurance Unit Representative. 9.2 The final report will consistof at least the followinginformation: 9.2.1 Title page, including the study title,data reqc4@rement, author, study completion date, testing facility, and sponsor. 9.2.2 Sponsor-suppliedconfidentialityclaims. 9.2.3 Good Laboratory Practice Statement. 9.2.4 Certificationof Good Laboratory Practices. 9.2.5 Table of Contents. 9.2.6 Sumary of test procedures and results. 9.2.7 Introduction. 9.2.8 Methods and Materials, including a description of test methods, organisms, dilution water, and statistical techniques. 9.2.9 Results, including all data from range-finding and definitive tests, and ECSO values. 9.2.10 References. 9.2.11 Appendices,includingall water quality data. 10.0 REFERENCES OECD. 1984. OECD Guidelines for Testing of Chemicals. Section 2: Effects on Biotic Systems. Method 202, Daphnis sp.. Acute lanobilisationTest and Reproduction Test. Adopted 4 April 1994. U.S. EPA. 1986. 40 CTR Part 797. Toxic SubstancesControl Act Test Guidelines; Final Rules. Federal Register, Monday. January 6. 1986. Section 797.1300. U.S. EPA. 1987. 40 CrR Part 797. Toxic SubstancesControl Act Test Guidelines; Amendments. Federal Register, Wednesday, Nay 20, 1987. Section 797.1300. Pago 5 of 6 M*ft kv- SAD%dKxv d klIPM Envir*Systess Study Nwaber 90171-3 SUNWT OF TEST C01FDrTIONS Acceptal)le Species: Test Type: 'restDuration: Flow Rate: Dilution Water: Age/Size of Test Organisms: Acclimation Period: Temperature: Pbotoperiod: Light Intensity: Number of Concentrations: Dilution Factor: Number of Replicates: Number of Organisms/Replicate: Test Vessel Size: Test Vessel Volume: Solvent: Loading Rate: Feeding: Aeration: Effects Measured: Test Concentration Analysis: Water Quality Measurements: Acceptability Criteria: Daphnis Magna Static 48 Hours Not Applicable Natural Ground Water Juveniles; Less Than 24 Boars old Continuous 20 :t JOC 16 Hours Light/9 Hours Dark Ambient Laboratory Ligbtinff 5 or Nore; less than 1,000 mg/L Si.6 4 or Nore 5 250 al or larger 200 al or more Dinethylformaside. Acetone. or Trietbylene Glyml. Less than or equal to 100 ugfl (0.5 g/L None During Exposure None Death; Immobilization None DO, pH, Temperature and Conductivity at 0, 24, and 48 Hours nO% Control Mortality, Stress, or Immobilization Acceptable Teaperature Range Page 6 of 6 RemLomProvft Wke-Sib"md K*LPM PRODUCT REGIS@.RATIOIN LIBORATORY SAMPLE RECEIMG tOG 1. RAI Sample number 2. Sponsor Name and Address G.Ps L +,Ic. ps!dzl oUAi 3. Sponsor Sample Identification )4arq-(. *A -Cp0 4. Sample Container Label Data F S. Date Received ll-t5-5o 6. Date Photographed 7. Method of Shipment Shipped from r,4. Via 8. Description of Shipping Container(s) (Number, Type, Size) IV% fj c 9. Description of Sample Containers(s) (Number, Type, Size) Aso 0-i S"s ;Otl-Lc 10. Sample Storage Location and Required Storage Conditions p(tt-#I' 11. Signature --g& I-. v 12. Notes Date 13. Date and Location of Archive Sample lemoval and Storage Location -io ih:SISA CERTIFIED TRUE COPY. SIGNATURE <ftn DATE a-3-i0 Ce%OL$Ce A6-CVft 'rC. SUbSC@--d Cl %@UPOrf PRODUCT RZGXSTRLWXCN CiDI'4-i3 LLBOY@ATORY TZST SUBSTLXCZ USKGZ LOO ni SMTLE =nn =T smwrlm_-.:.-FA& JM& TEXOM An ZERSU33M xitGRIM DATE IN=ML GROSS FM GROSS ANOMIT DISPOSITIM By VE3EGRT MaGgs RZNOM tt-t&-tc, its3 .;t qsl.2 1-0. A4-,E,'tt AC TRU RE DATE -oss Sample Description: Container Label Information: Date Received: Date Of Return To Sponsor: 003OL-ce ar-ov@l. irc. Situe-av el MUPCot species: Day of Mouth PRODUCT ITEGISTUTION THIS IS ACERTIRF-D TRUE COPY. SIGNATUREc5oi4--DAIIR 01*c%lii LABORATORY OITCANISM IcamaTiow number of Dead rOO4 reeding Frequ4neY Lot NO. 11"9,01 I%W& D.O. Sal. '*C Cag/L) (Ppt) igotes Loc BY/Date 2 3 4 5 6 7 8 9 10 12 13 14 -15 16 17 is 19 20 21 22 23 24 25 26 27 28 0 v-:S I)C LW 29 O)L Lm 30 31 PROCUREMENT DATA: iLge-AA1.1-hr-Condition $gm,4 Source. Collection and/or Transportation IVetbod: Date pow .4g-^ IVIZ&Lq6 Number Procured: Notes: WATER QUALITY: D.O. PH mg/L At Shipping: At Arrival: Temp. OC Salinity pot Conductivity nabo/cm ACCLIMATION DATA:Vater Source 1Aa]&4 Food and Lot@t.- Note: Include ifiyob-sertation6f dfiseaseor abnormal behavior. '@'se additional sheets if necessary. Species: Day of Mouth PRODUCT RECISTRJLTION LA30RJLTDRY ORGMSN ACCLMTION Number of Dead Food Lot No. 11.2r461 /it reeding rre- D.O. Sal. quency OC (mg/L)(ppt) Notes LOG By/Date b--- L9- 2 1= 3 IlL LL-Z 4 tx -*V-IL 5 L45 814 2f).I. 6 4,j /,c idr d* u a 7 .f) ud I%t a f) L IN 9 tie FJA A.Z-1-11) "4 tL'7- k r -fi@p itFc 0 -7-,% 10 1 It 11 I'L 12 w- 13 015 fx 14 a A/A I A)A 16 It til+ t -ILiT& 17 iw li,9 jz-c?-,I& im 19 a a-tr 20 21 -ugrsrra A 22 74 23 G%L4ATIIn;: 24 25 26 27 29 29 30 31 PROCUREMM DATA: Age _ Condition Source, Collection and/or TransportationMethod: -a *amftAAad Date Number Procured: Notes: TATER QUALRTY: D.O. PH MILL At'Shipping:-At Arrival: Tonp. OC Salinity -opt Conductivity usho/ca ACCLIMATION DATA:Vater Source food and Lot bc SLAXNSCaWLMISCM mote: Include any observation of disease or abnormal behavior. Use additional sheets if necessary. Resource knalysts, Inc./ 'EaviroSystessDivision Product Registration Laboratory Raw Data Study Number qh 7r - -i@ Sample Nwaber A&tf& i!@-7 Test Substance iei-o Test Type: screen _L/ def in-itive TEST CONDITIONS Da to A. Test* Organisa7). 110a Recorded by Lot Number Test Duration 4/h/i Dilution Water /IZ&W-/ af2" Age and Size of Test organis 400'02 C& 4 Flow Rate S4Lliuity/ Hardness LR- 14V -el9D IA449 u Test Organism Abnoraa]Lities. iiin@ss, or Injurie's:none_j some (note)- Acclimation Period (length at test initiation in days) tf4 Temperature Photoperiod: time on4k&Q Incubator Nuiaber 1-4- time off Light Intensity uein 15 Minute Transition Period: Yes No Number of Concentrations Dilution ractor Number of Replicates Number of Organisms per Replicate Test Vessel Size tL) . 15?1 Test Solution Volume (L) Jec Test Solution Depth (ca) Solvent and Solvent Concentration -p- ubnirt- Loading Rate Fee&Ln% 1442 Dissolved Oxygen Levels ->-Io,07. Effect(s) Measured Test Concentration Analysis IX41)tl Vater Quality Measurements Icsource 3.*&2yzts,isc./ xzvicasystczx virislan :Product legistration LaborAtory law Data Study xumler f4A---4 saxple xwLNer Te3t Sulsta=ce J-64 Test Tr,@*:xcreaa deiinitive S'KOCX SoLtrrioic?X"J=7709 ?ercent. active izgrcdlczt (% jLX) )(azt:tnaC3o.ncentrat:icns corrected for % JLX 2 yes -no BaLlanced V*ed for Stock SolUticA ireparatioll &t. Preparation Notes.- _let Recorded by: --f4ad,@@ &MTUA /lw V-wq/ Date I,? Cw.Xne Ar@ VV- S.&%4CPv 0 UC*"YX:I- Resourcelnalysts,Inc./ EnviroSystessDivision Product Registration Laboratory law Data Study Number 401-yl- a Sample Number Test Substance 14/9 Test Type: screen L/ definitive CM(EM INFORNTION Notes: EnviroSystemIsn.c.S.@h. =r,,o4@,IILL!PCRE ResourceAnalysts,Inc.! InvirOSY$tessDivision ProductRegistrationLaboratorylaw Data Study Niiaber IPA /I,/ Test Substance Sapple Number-.u4/oeTest Type. screen&,/-definitive- DAPHNID SOLUBILITY rnitiationDate Test Organimd.,mctw,',- Test Vessel I.D. Control Insoluble Naterial 0 bLr 24 br 43 hr Recorded by FA Date: If no insolubleitaterialis observed enter a 2ero in the appropriatebox. If insoluble aaterial is observedenter a cbeckmark in the appropriate boy and describe the observation below. Note-$: Resource Analysts. Inc./ EnviroSVte"s Division Product Registration Laboratory law Data Study Number j,//.3 Sample Number Test Substance Alt Test Type: screen 2 definitive DAPHNID SURVRVAL AND SUBLRMAL ERF=S Page I of 2 Initiation Date lz-5-qo I!est Organism *'-)i-2Lgj Test Vessel I.D. Rep. r of Live DapnbTn8Si,ds,,bl,,Sublethal effects 0 !kr 24 br 48'*bdrs 0 br 24 hr 48 hr Colmtrol 2 1 2 r 0, 1 2 1 2 2 5 4 .o 0 0 0 0 0 0 n- 1 0-1 I 6 2 6 10, 2 Recorded by: 6L Date: 1; if -no sublethal effects are observed enter zero in tbt appropriate sttblethal . effects -sro observed enter the nppber effpcted ap!)ropriate box and describe the observation below. box. If in the ACO:I.wV%LcSZ!-,3ccrto%MLIPM Resource Analysts, Inc./ InviroSysteps D;-wision Product Registration Laboratory law Data Study Pu*ber Sample ?lumber Test Substance -@*Z Test Type: screen -,,/definitive DAPKNID DISSOLVED Initiation Date OXYGIX CONCMRMATION 17--5-qo AND ps Test Organism Page I of 2 Test Vessel I.D.' Rep. Dissolved ozygen tag/L) 0 hr 24 hr 48 br 0 hr PH 24 br 48 hr Control 9.5- 2 1,4 2 0 sshorlw, 7-7 7-o @-0 I-o 9.!5- 9-c 2 2 2 2 Recorded by: Date: -7-1 g-,5- r-r. 3.@ 9-c> 8,3 1 C) 114 19.tr 7-o F-4 rs- r-t -C> Notes: Tnstrtiment Ulimber: Reu>-dr-AeRCNVL Wt. SL@ Cl Wiscuret Analysts, Inc./ EnviraSyste"s Division Product legistration Laboratory law Data Study Number Sample Nupber ?/,,4 f e, Test Substance Test Type:screen /definitive- DAPMD TENMATURE AND COWDUCTrVnT Page 1 of 2 Initiation Date Test Organism Test Vessel r.D. Control Temperature (OC) Conductivity t /es) Rep. 0 hr 24 hr 48 br 1 0 hr 24 hr 48 br 2bl II&- 17-14 /,/oo 134oo 2 g -" I 2b9 I It. ]1-'5 AIW r3 a-.3 11-4 "-1 /:Ue f-Izk)L?d.3 2 @0-') 11,k. 11--l , .2 2 1 2 2 ILI Recorded by: Date: Notes: 19-(, 1300 ) C2 >4-1 /*h -to.o Mo A;-S,'fo Instrument Nu!%bir: I69im@91 "L gesctoceAr<ovv& W-C.sub%3.--,fd KMLUPORE Ras ttudy Number Test Substanct tim Lubmatol;uv Test Ty": screen @le ZNOR CODICS 1. Nisspened 2.;Hathematical Error 3. Wrong ]@zltrr 4. Trainositim Or S'eq'o@amcf@a-zrrm 5. Transcription.Chmis@.teoPi eiT=) 6. Procedural Changi' 7. Wrong Conclusion a. niegible Intr3r 9. Ulmeceskar3r I:ntr3r io. rootnoted zxpl=ation 11. Additional Co--cut rill. Resourc* Analysts, luc./ Envir*Syzt*as Division Product Registration Lab*ratary Raw Data Study Number 5b6 Test Substa=A Sample Number 4%,Iq(fq --7 Test Type*.screen - definitive TEST CONDITIONS Date Record ad by - VA Test Organism 7,>co Lot Number Test Duration 44AOr. 72ow Rate A)# aliniLty/ Dilution Water SWduess v 4.441,r. Age and Size of Test Organisms. Test Organism Abnormalities, Mness, or InJuries: none /some (note) Acclimation Period (Itngth at test initiation in days) Al,# Temperature .2611 incubator Number 12- Photoptriod: time an 4kM time off -P3.-2 a Light latensity Z@__-Ein 15 Minute Transition Period: Yes No %/ Number of Concentrations -J"rtiAlya6z Dilution ractor Number of Replicates Number of Organisms per Replicate Test Vessel Size (L) 4> a2 5@' Test Solution Volume (L) -2-6 Test Solution Depth (cm) 4 Solvent and Solvent Concentration Loading Rate 4 feeding Dissolved Oxygen Levels > Effect(s) Meas%xred -CA@alm Test Concentration Analysis Li# Water Quality Measurements?)6 7,4 Pd,"-t 2 Resource Analysts, Inc./ invirasystessDivision Product Registration Laboratory Raw Data 'StudyNumber -Isb5 /Pt A, Al-It-701 Test Substance Sample Number Test Type: scree7 definitive RAMNIZILTION IMRIMTION InitiationDate- Test Organiss Test vessel I.D. Vessel Vessel Rep. L*ca- Rep. Loca- tion tion 3 a 2 4 1 3 2 4 1 3 2 4 1 3 2 -43 4 1 jj 3 2 4 1 3 2 4 1 3 2 4 Recorded by: Date: Random Numberss $8759 35661 26335 6026 9@94 83746 27999 $268S 18386 gpo ig446 66027 S14& 2704S 13094 92382 16215 09342 38143 23689 25407 25349 02322 15072 27002 66181 09779 10791 74833 17593 45601 60683 29956 91713 666& 428312 t4774 98396 47694 42562 3&3 23862 13141 830S2 75177 96779 62626 1772S 62Sl8 SD$09 14528 00 3;97 37726 69456 77491 33261 31036 $3316 01822 07706 55767 24038 46977 33112 $2169 8423S 33943 (GaO 4CW 56527 0763 0&43 63 7462S tiass gicv 11342 47398 5430973159 1430 177S2 49326 64727 03509 72382 73099 19693 5609S 99219 SS278 40386 45537 $7481 31312 83701 39325 65995 19877 75296 Draw- a circle -around the sectionof the randomnumbers tablethatis used to position the test vessels. Table of random numbersis from Steel,R.G.D and J.H.Torria. (1960). Principles and Procedures of Statistics.McGraw-HillBook Co. New York. Page 428. ROWMO PJX*ftIMCS.ibsc@ady L%LFM Resource Analysts. Inc./ invirosystess Division Product Registration Laboratory law Data Study Number 5b.5 /ec@* Test Substance Sanyle Number Test TM: screen definitiveiz STOCK SOLVTION PREPAUTION Percent active ingredient (% Nominal Concentrations corrected for % Al ? yes L/ no Balanced Vsed for Stock Solution Preparation Preparation Notes: IAOLO xAd Zcy2L a.,tal 4V-7 Itrll MIS LO, ,2 Recorded by: Itp 11 Date Rewume NWWL tr- SjbK" of WIPM Resource anairsts. Inc./ uvlroftstem Division Product Redstration Laboratwi Uv "ta Study Nunber Test Substance /6 t Fr /-w t%o samplemm:b@er Test 71": screen definitive GMFEM limvmtlon Notes: -h Pel- 7- &A Ig etl=@Lf- lo@ I / En@;e*SyslIenmcs.S..k:s;c,=vcf.VAUFI-IRE Resource Analysts. Inc./ towirosystessDivision Product Registration Laboratory Raw Data Study Number Test Substance SD 5 /i@CJW I"r4di Sample Number V)N --t4-3likli Test Type: screen definitive DAPENID SOLUBRL M Initiation Date ?e, Test Organism Test Vessel I.D. Insoluble Naterial 0 br 24 hr 43 hr D Recorded by 4E5 Date: . If no insoluble material is observed enter a zero in the appropriate box. If insoluble material is observed enter a check mark in the appropriate box and describe the observation below. Notes: WesoLoMcKe*NL Wc.-Azsccrdv%ftL E Study Nuaber Resource Analysts, Inc./ EnvirosystessDivision Product Registration.Laboratory Raw Data Sample Nwkber IJN -(U-)14@ltq Test Substance Test Type: screen - definitiveJ/ DJLPMIID SURVIM AND SUBLETRILL EffECTS Page 1 of 2 initiation Date Test Organism Test Vessel! Rep. I.D. 2 3 4 -.2 3 4 1 r of Live Daphnids 0 hr 24 ILr 43 hr 65 15 5 q !5 55 41 5 57 Sublethal affect& 0 hr 24 hr 43 br 0 C) 40 0 0 0' o C) o 0 6 0 2 3 4 15 1 32 3 15 4 Recorded by: Date: C) '5- -1- --0 71 6 16 r) 1) 01 L) gp@-j C/P" 1 1.2 1) 9 Resource knalysts, Inc./ EnvirosystessDivision Product Registration Laboratory Raw Data Study Number -57)-5 - Sample Number 0 -t-@ 14C-Ar- Test Substance Test Type: screen _ definitive DAPENID SURVIVJLL AND StMLETRkL EFNCTS Page 2 of 2 Initiation Date W-90 Test Organism (I - yffia C4@- Test Vesseli I.D. Rep. Number of Live Daphnids 0 hr 24 hr 49 bLr Sublethal effects 0 hr 24 hr 48 hr 1 2 57 0 3 5 0 0 2 3 4 2 3 ,o Recorded by: Date: If no sublethal effects are observed enter zero in the appropriate box. If sublethal effects are observed enter the number effected in the appropriate box and describe the observation below. Notes: C.1IA-i@-e44 Resource Analysts, Inc./ EaviroSystess Division Product Registration Laboratory Rav Data Study Number /et Ae ALI:,qd I Test Substance Sample Number 5,14@Rl Test Type: screen _ definitive DKPIMID DISSOLVED OXTGZN COXCMRMTION = PI Page I of 2 Initiation Date Test Vessel Rep. Test Organism Dissolv ge (av/L) 0 br 24 'hr 49 hr 0 br 24 hr 43 hr 1 f(-o @o-scl", 2 9-0 3 4 DitD-00 1-7 C-) q 8-5 2 3 4 K. z1 @-,z. I 2 r(-q 3 tf3-3 _111.--7 f. 6-5 7.-7 &-(5 2 3 4 Recorded by: Date, 9'0 ? iik.lif) lfqf,I)dU#o 1)-ff_qo ResouiAcreolv&M Irc ske t clMLUPM Resource Analysts, Inc./ EaviraSystess Division Product Registration Laboratory Raw Data Study Nuaber 5D5 /a.c* I.Urqd f Test Substance Sasple Nuaber Test Type: screen definitive DAPM91D DISSOLVED OXYGEN CONC=MTION AND pE Page 2 of 2 Initiation Date /4 -/g'-'Fo Test Organism Test Vesse]L X.D. Rep. I Dissolv Oxygen W94--s 0 bc 241hr (ag/L) 43 hr 0 hr 24 hr 48 hr 4 @rg0q-q 1 2... 3 41 8'0 1 T.L) 2 V7 1-7 g@3 9--5 4 Recorded by: Date: 47A Notes: ResoL*ceAr4vft ,,cS.ubuj=V d MUJF;M Resource Analyst$, Inc./ EavirosystessDivision Product Registration Laboratory Raw Data Study Number Sample Number iu -jig(ctt Test Substance Test Type: screen _ definitive DAPMD TEXPERILTURZ AND CONDUCTIVRTY Page I of 2 inittation DatT Test Organism Test Vessel I.D. Rep. Temperature (OC) 0 hr 24 hr 49 hr canductiv@g V.4 I 0 br 24 bLr os/ca) 48 hr I ZO-1 2D-V zo.r 116b 1360 13L7() 2 ;O_r )0.1 '111). Uho 13M 13W 3 ;6.'7 X,9 -u-I I@00 1300 1306 4 IXo ;O-r 7z;, I I1(m (300 1400 I A @ :?0 I)Ab --1300 1460 -.2,. - --?o f jlm- 13M 1 / 4&(,Dv 3 -10.9 10 -f&Y-) 4 X7 .2o.9 2 3 4 1 32 3 4 Recorded by: Date: iz-1 120.1 ;Iaq IX-9 16-@, ;b.I M-9 ;0.1 @i-a At).qjc)y i-e.? -JO.IZ @og 30 q ;0,9 flooI?oo Ik)D 190 tk4D 14AO lxne. Ir-C LA6b 13(D 13(io -1@m /&io 14-)o W) IJff-* 'Ab otMLUPORE 1020UrCO AnalySt2, Inc./ Eaviro$ystessDivision Product Registration Laboratory Raw Data Study Number Test Substance DAPSKID TENPMTURZ InitiationDate MD CONWCTMTT fo Sample Number PN Test Type: screen _ Z definitilre Page 2 of 2 Test Organism D. IvAaA-& Test Vexxel I.D. Rep. Temperature (OC) 0 bLr 24 hr 43 hr ConduCt-ZV3-L;r (wom/cm) lqej-- 0 br 24 hr 48 hr I @6.1 2 -9@% 3 4 2 -- ;b.% 3 @6 -9 -W-1 4no - 136o t@oo IIOD 1100 1w 26"-7 .70.4 iim 13QL 1460 11(v L2LV f,@o v.o .,:a.1? 4 I 2 3 4 LI Recorded by: Date: Notes. Resmocehxoift wc- &Zsc"y clNSIF<)K I"aurce analysts. Inc./ Eavirosystow Division Product R,*gistration Laboratory Raw Data HARDNESS DETERMINATION Date water type al EDTA aegquCiavCaOl3ent missasple Hardmnge/sls as BY titrant C&CO3 to 1.00 EDTIL Mg CaC03 equivalent S.Oal CaCO3 ml EDT Hardness (mg/L CaCO3) al EDTA titrant mg CaCO3 x 1000 al a sample equivalent Resource Anal3rsts. Inc./ ZnviroS3rstess Division Product Registration Laboratory Raw Data Study Number s Test Substance Sample Number _Fj . Test Trp e: screen -t") definitivejz NUOR CODRS 1. misspelled 2. Mathematical Error 3. Wrong Entry 4. Transposition or Sequencing Zrror (inverted or vrong order) 5. Transcription Change (copy error) 6. Procedural Change 7. Wrong Conclusion 9- Megible Entry 9. Unnecessary Entry 10. Footnoted Explanation 11. Additional Comment Resource knalysts, Inc./ EaviraSysttms Division Product Registration Laboratory Raw Data Study Number clo)Il-3 eTest Substance rmz Sample Number 2(ot(?o Test Type: screen definitive TEST CONDITIONS Date - /2 Recorded By Test Organism Lot Number /.I/ Test Duration t@r-f Dilution gater tpr Age and Size of Test Organisms Flow Rate I)t4 SiiLlinityl Hardness - i @q@l-. Test Organism ILbnormalities,nlness, or Injuries: nonez some (note)- Acclimation Period (length at test initiation in days) A)A Temperature 78!1 Incubator Number I'L Pbotoperiod: time o @M time off7,2a2 Light Intensity 73__uEin 15 Minute Transition Period: Yes No Number of Concentrations Dilution Factor Number of Replicates Number of Organisms per Replicate Test Vessel Size (L)-aZ5 Test SolutionVolume (L) OZO Test SolutionDepth (ca) Solvent and Solvent Concentration Loading Rate L 0. L Feeding DiIssolved Oxygen Levels '7 '7()In tit " Effect(s) Measured tical -'@ij Test ConcentrationAnalysis L)14 Vater Quality Measurements Study Number Resource Analysts. Inc./ ZaTiroSrstms Division Product Registration Laboratmy ]tax Data Sample lumber er- Test Substance i*X -3,F!2z Test Tnw: screen definitivit RANDOMMTION INRORMILTION rnitiation Date lp-Ir--qA Test Organism Test Vessel X.U. Rep. Vessel liocation Rep. Vessel Location 1 3 2 4 /1/ - 3 2 4 1-- 3 2 4 1 3 /17 2 4 1 3 2 4 1 s3 2 4 ';@ 4 3 2 4 Recorded by: Date: Random NwiLbers* $9758 35661 26335 60326 950" 83746 27998 $2685 13386 21717 19"6 66027 5140 21"5 13ff94 92382 26215 09342 38148 23689 25407 25349 02322 15072 27002 66181 09-[79 10791 74933 17583 4S601 60683 29956 91713 66605 42832 03771 74718 98396 47694 42562 321P 138 2 13141 glft 7507 96779 62626 27725 6 2,-kl3 5069 14528 79001 19997 37726 69456 77491 33261 31036 $3316 016# 07706 55767 &38 46;D 33 81169 $4235 33843 (%40 4 56527 13763 06f33 63tC 74625 (Rgu iilw 3042 47398 !54309 73159 1430 17752 49326 64727 03509 72382 7AO99 dA93 56095 9909 SS278 40386 45537 874;1. 31312 8370 39939255 65 18877 7S296 Draw a circle around 'the section of the random numbers table that is used to position the test vessels. Table of random numbers is from Steel,R.C.D and J.H.Torrie. (1960). Principles and Procedurox of Statistics. McGraw-Hill Book Co. New Tork. Page 428. Re=Lnm AZCWL Vx.&AMK$OVClkNiFORE ResourceAnalysts.Inc./ Invinarstow Division Product Registration Laboratozy Raw Data Study Number fol-IlTest Substance oc@ok f I'Ao Saxple.lusUr Test Type: screen definitive J2 STOCK S016UTION PRZPARXTION Percent active ingredient (% RI)IM Nominal Concentrations corrected for % RI ? yes no Balanced Used for Stock Solution Pre W ation Preparation Notes: Zt)" 14Al-4Ajd-, - I t/- a@z@@ /2) &ONW;x@ Recordedby: Date 19-If-?(3 RoWL*ACReCWLVC.$A-4aVCO%WJ$40K Resource inalists. Inc./ EaviroSistansDivision Product Registration Laboratory Raw Data Study Nuaber qt)lltl--l Sazple N=Llier 4,f&c Test Substance -VAZ& Test Type: screen definiti"i@/- GENERJLL 330ORK&TION Notes: AM,-I-ZAIAIJAk4 Adz& lzt lq LOo C4- A ,)A-,2).A 0 IIA Study Number lqsoures Analysts, Inc./ ZaviraSystessDivision I!roductRegistration Laboratory Raw Data 9hifl --3 Sample Number Test Substance . Alt, ;Irzo Test Type: screen _ definitive DJLPM[ID SOLUBiLLL-El Initiatim Daft- Test organimz) Test V*Ssel Insoluble Naterial 0 hr 24 hr 48 hr o o 0 oD o 0 0 Recorddeedd bb y Re4 Dattceeo-:r. If no insolublematerial is observed enter a zero in the appropriate box. If insoluble material is observed enter a check mark in the appropriate box and describe the observation below. Notes: woux" ARCWL kC. SUB$KUDV OfNILUPORE IN I Study Number Resource Inalysts, Inc./ EnvirasystessDivision Product Registration Laboratory law Data e), Sample Number Test Substance ArIx -ilf.2.6 Test Type: sermm _ definitive V/ DAPMIID SURVML AND SUBLETUL EMM Page I of 2 InitiationDate Test Organism Test Vessel I.D. Number of Live Dapbnids Rep. 0 hr 24 hr 48 hr Sublethal effects 0 br 24 hr 48 hr 2 c3 3 15 25 3 r2 4 r5 1 -15 .2- 2 5 3 13 4 1 3 2 3 4 Recorded by: Date: 5' -.-,r 15 5 !5 ->r L4 5, 5 '5 5 TO c5 0 8 0 o C) 0 Li o CYNJ 6 c) 0 .2- 0 0 6 qp---_ei /,J Z-t7-t-q Subs".ovd RAL$@ORE Study Number Resource analystx. Inc./ Eavirosyste" Division Product Registration Laboratory Raw Data ?Ii,1/--3 Sample Number -7&,96 6:,- Test Substance I-Irlt Test Type: screen _ definitive DILPM#ID SURVRV]LL AND SUBLETNL EFFECTS Page 2 of 2 Initiation Date...-lal-Ifr-'Vi) Test Organisn D@ Test Vessel Rep. Number of Live Daphnids 0 hr 24 hr 48 hr Sublet%al effects 0 hr 24 hr 43 hr 1 15 2 '5 3 4 evo 0 00 LI) 0 0 . ....... 00 3 4 2 3 4 Recorded by: Date: If no sublethal effects are observed enter zero in the appropriate box. If sublethal effects are observed enter the number effected in the appropriate box and describe the observation below. Notes: F- -fo 4 Reso=e AICMM VC..&AnKbOV d %ILLFM Resource Analysts, Inc./ EnviroS"tess Division Product Registration Laboratory Raw Data Study Nuaber fz@o//--S Sanple xu"er - Aeft.,E Test Substance fIx Test Type: screen - definitivez DAPM(ID DISSOLVED OXYGO CONCMffUTION AND pR Page 1 of 2 Initiatim Date Test Vessel I.D.@ Rep. Test Organisa Di2SOlV 0 hr d ox!rae (291L) jolel-.q- 241 hr 48 hr .0 br 1 24 hr 48 hr I 2 t3 3 s-5 7K 2 3 4 -7-Y S-1 G 2 3 Y.'7 f-; 32 3 4 Recorded byDate: Reo=e ACMft 'rC&.jbPCKYdVMUPM Resource Analysts, Inc./ Eavirosystems Division Product RWstration Laboratory Raw Data Study Nuabtr Sample Number .2& 1?4g.- Test Substance .1@'IN-Va-a Test Type: scr*en _ definitive DkPMtID DISSOLVED OXYGEN toNCMnIATION AND pa Initiation Date Test Vessel I.D. Rep. I 2 Test Organism Dissolv 0 hr oxygen -4 24 hr (mg/L) 48 br .0 hr T"), g.? -0 Page 2 of 2 24 hr 48 hr 3 4 1 2. 3 7,r 'f3. 6--(o 4 2 3 4 R ecorded by: Date: Notes: RE=WCO ARCW& WC.kOSKI-=O@fM,UIFRAE Resource kualyxts. Inc./ tovirasystess Division Product Registration Laboratory law Data .Study Number Sample Number --21.qol;.- Test Substance Test Type: screen _ definitiv*L/ DAPRX,ID TEUEPATUN An CONDVCTIVM Page 1 of 2 InitiationDate A.2:rZ -1,> Test Organism Test Vessel I.D. Rep. Temperature (OC) 0 hr 24 bLr 4S hr duct vity (umbes/cm) a br 24 hr 49 hr 2 3 4 2 3 4 I 2 3 4 I 3 4 Recorded by: Date: 111-0 )0,9 -11% 21-,) I[,0 Y' lo,-] IO-Z ;0.7 ;OA 40,1 1zo.I -2j-i) lim IJ OD p I@,Lt) t;L)Q 1;00 1' lzco 1-@W 1;00 lko 13-6, 'wI 124D 4k q d-O,Z .20-Y kl- /Lao 1300 i;co IkO t4oll f6 owo=e A-c@vft imc SANCov d LU.LIPM Resource Analysts, Inc./ EnviroSystms Division Product Registration Laborator3r Raw Data Study Number 40.-of-l Test Substance Sample Number Test Type: screen _ definitive DAPMFID TMEPMLATURC - Initiaiton Date Test Vessel Rep. I.D. 1 2 3 4 3 AND CONDUCTNM Page 2 of 2 Test Organism 2@a.aea@ Temperature (OC) coanctY"e''t-@Ir ( 0 hr 24 hr 484C)@8hrhr 0 hr 1 24 hr os/cm) 48 hr A-9 A-9 @0,7 9-t AD-Z k& ),or W).q Ar @7i. 1)6D lio JO-1 1260 -zol I)-oo 2,o 11-4 JIM );OD 1-300 1,400 I;oo 1-'(D f 4 j@/ 1 I ilw lip) 2 3 4 Recorded by: Date: Notes: Remuce ArcVft kc..Sub-"y d LMIFOK Resource Analysts, Inc./ EavirosYstessDivision Product Registration Laboratory Rav Data B;LRDRESS DETERXMTION Date Water type. al EDTA titrant ag CaCO3 equivalent to 1.00 IEDTI MIS sample Rardn;;ss By mg/l as C&CO3 do mw4r-to 444,li@o mg CaCC@3equivalent 5.Oml CaCO3 al EDTK Hardness (mg/L CaCO3) ml EDTA titrant x ag CaC x 1000 ml al sampleIC equivalent el Resourceimaysts. xnc.tzaviros"tms Division Product RegistrationLaboratmi Raw Data Study Number aep-21-3 s=ple Number o?Zo47& Test Substance Test T"e: scrom definitive_L/_- ERROR CODES Hisspolled 2. NatbesaticalError 3. Wrong Entry 4. Transpositiono*r SequencingError (invertedor w=g S. TranscriptionChange (copy error) 6. Procedural Change 7. Wrong Conclusion S. Ill*gibleEntry 9. Unnecessary Entry 10. IrootnotedExplanation 11. Additional Comment Order) - ---------------LC VALUES IT IS IMPORTANT THAT ALL RESULTS BE COMPARED WITH THE ORIGINAL.DATA..TO.SEE.LF THE RESULTS SEEM REASONABLE. MOST. IF NOT ALL, METHODS FOR CALCULATING LC50 VALUES PERFORM POORLY WITH SOME SETS OF DATA. ALTHOUGH MANY PRECAUTIONS HAVE BEEN INCORPORATED INTO THIS PROGRAM, SOME QUESTIONABLE RESULTS MAY BE PRINTED WITHOUT A CAUTIONARY STATEMENT. 471) AP L f-SD ------BINOMIAL TEST CONC. NUMBER NUMBER PERCENT EXPOSED DEAD DEAD 50 20 20 100 25 20 10 50 10 20 3 15 5 20 3 15 1 20 4 20 .THE BINOMIAL TEST SHOWS THAT 10 AND 50 CAN BE USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS BECAUSE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT BINOMIAL PROB.(PERCENT) LESS THAN 0.001 58.8098 .1288413 .1288413 .5908963 25 CAN BE USED AS AN APPROXIMATE LC50 FOR THIS DATA SET ---------RESULTS CALCULATED SPAN 4 3 2 1 G 7.809802E-02 8.00997SE-02 .0864314 .7002976 USING THE MOVING AVERAGE AEC--O 9) 7*J -t:c_-Oo tql 95 PERCENT 15.58225 17.89105 20.97725 10.89568 13.92568 16.68199 25 16.9624 METHOD-- CONFIDENCE LIMITS 23.e476B '-7'3.4855-.7 --5.78795 313.4993 AN LC50 CALCULATED USING THE MOVING BE A VERY GOOD ESTIMATE IF THE SPAN NUMBER OF CONCENTRATIONS. AVERAGE IS MUCH METHOD MAY NOT LESS THAN THE IF ANY HIGHER CONCENTRATION PRODUCES A LOWER PERCENT DEAD THAN A LOWER CONCENTRATION, THE CONFIDENCE LIMIT AS OBTAINED BY THE MOVING AVERAGE METHOD WILL PROBABLY BE TOO CLOSE. ---------RESULTS CALCULATED ITERATIONS G 4 :3.296692 USING THE H 7.061959 PROBIT IAETHOD---------- CHI-SQUARE PROBABILITY 21.18588 LESS THAN 0.001 BECAUSE THE PROBABILITY IS LESS THAN 0.05. RESULTS CALCULATED USING THE PROBIT METFIDD PROBABLY SHOULD NOT BE USED. SLOPE - l."9584'3 95 PERCENT CONFIDENCE LIMITS = -1.0569q2 AND :;.648677 POITIT cot4c. LOWER UPPER 95*" CONFIDE@ICE L-T.-IITS LC VALUES r IS IMPORTANT THAT ALL RESULTS BE COMPAC-ED WITH THE RIGINAL DATA TO SEE IF THE RESULTS SEEM REASONABLE. 3ST, IF NOT Al.L, METHODS FOR CALCULATING LC50 VALUES =-RFORM POORLY WITH SOME SETS OF DATA, ALTHOUGH MANY RECAUTIONS HAVE BEEN INCORPORATED INTO THIS PROGRAM, OMe CLESTIONABLE-RESULTS MAY BE PRINTED WITHOUT A AUTIONARY STATEMENT. 49 At-6cdb Z/al'tt ----------------- --BINOMIAL TEST ONC. NUMBER NUMBER PERCENT. EXPOSED DEAD DEAD 150 20 20 100 100 20 20 100 60 20 14 70 40 20 6 30 @-)5 20 0 0 -HE BINOMIAL TEST SHOWS THAT 25 AND 100 CAN BE ISED AS-ST4T,I-STICALLY--SOUND CONSERVATIVE 95 PERCENT 'ONFIDENCE LIMITS BECAUSE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT BINOMIAL PROB.CPERCENT) LESS THAN 0.001 LESS THAN 0.001 5.765907 5.765907 LESS THAN 0.001 4N APPROXIMATE LC50 OF, 48.98977 4ONLINEAR INTERPOLATION BETWEEN IS OBTAINED BY 40 AND 60 ---------RF-SULTS.CALCULATED USING THE tiOVING AVERAGE METHOD--- :-;PAN 4 3 2 G .0422766 5.4BOl77E-02 .1234916 .1234816 .6117131 LC50 50.305B 49.29081 49.9a979 48.98979 48.913977 95 PERCENT 43.4101B 43.61243 39.82044 42.74617 @@7.98:545 CONFIDENCE LIMITS 57.'->5496 55.76t.142 56.50342 59.71668 6z.le534 AN LCSO CALCULATED USING THE MOVING DE A VERY GOOD ESTIMATE IF THE SPAN NUMBER OF CONCENTRATIONS; AVERAGE IS MUCH METHOD MAY NOT LESS THAN THE ---------RESULTS CALCULATED ITERATIONS-- G .1496121 USING H 1 THE PROBIT METHOD----CHI-SQUARE i.tu)4782 PROBABILITY eOO0949 SLOPE 7.77544 @@5 PERCENT CONFIDENCE LIMITS - 4.767922 AND 10.7e296 Poial CC. UO lw ,,t,E5t-) CONC. 4q.03664 LOWER 95% CONFIDENCE 47,.@-5647. UPPER LIMITS 55.857-@2 C-F PRODUCT CODE: F-7082kFM-382@,F-7460 --------------------- ------------------------------------------------- i'l. 0'66*4 14ATERIAL DESIGNATION: ISSUE DATE: 9/17/1987 FLUOROCHEMICAL SALT SUPERSEDES: OS/29/86 3H I.D. NUMBER: 41-2600-7082-0; 41-2700-3820-S: 41-2600-7460-9 NFPA DIAMOND CODE: HEALTH: I FliZE: 2 REACTIVITY: 0 OTHER: NO VUXFOR 24-HOUR EMERGENCY INFOR14ATION ON HEALTH EFFECTS CALL: (612)733-208219*z ----------------------------------------------------------------------------- 1. INGREDIENTS CAS NUMBER % TLV(R)CUNIT) ----------------------------------------------------------------------------- DIETKYLENE GLYCOL MONOBUTYL WATER FLUOROCHEMICAL SALT ETHER+ 112-34-5 7732-18-5 2795-39-3 CA 36 CA 36 28 NONE NONE NONE +SUBJECT TO SECTION 313 SARA TITLE III ----------------------------------------------------------------------------- 2. PHYSICAL DATA ----------------------------------------------------------------------------- BOILING POINT: VAPOR PRESSURE: VAPOR DENSITY(AIR-1): EVAPORATION RATE: APPEARANCE AND ODOR: ---------- --- ----- >212 DEG F SOLUBILITY IN WATER: <ISMM@RT SPECIFIC GR"ITY(H20=1): ND PERCENT VOLATILE: ND VISCOSITY: <100 PH: SLIGHT TINTED LIQUID; BUTYL CARBITOL ODOR ------ ------ ------- ----- -------- ---- SOLUBLE 1.08 72% CPS 10-10.5 3. FIRE AND EXPLOSION HAZARD DATA FLASH-POINT(TEST-METHOD):-172-DEG-F.-14IN.(CC)(EST.)--FLA.LMT.-LEL:ND--UEL:ND- EXTINGUISHING MEDIA: FOAM. DRY CHEMICAL, C02, WATER FO@ra. SPECIAL FI.RE.,FLGHTINa PROCEDURES*- NONE UNUSUAL FIRE AND EXPLOSION HAZARDS: NONE EXPECTED. BUT SEE ALSO SECTION 7. ----------------------------------------------------------------------------- 4. PRECAUTIONARY INFORMATION -- ------------- ----------- WEAR FULL FACE SHIELD. WEAR 114PERVIOUS GLOVES. ----------------------------------------------------------------------------- PRODUCT CODE: F-7082 FM-3820 ;-7460 PAGE 2 OF 2. S. HEALTH HAZARD DATA ---------------------------------------------------- ----------------------------------------------------------------------------- EYE CONTACT: CAUSES IRRITATI,'-.'iON CONTACT. SKIN CONTACT: MAY CAUSE IRRITATION. DRYING AND -:RACKING ON PROLONGED OR REPEATED CONTACT. 14AY'SE ABSORBED THRC-UGH THE SKIN. INHALATION: -== i TUGESTION: MODERATELY TOXIC ON ITIGESTIC-M. 694@@IJ-3qiESTEC, FIRC-T ATC-49*u EYE C--%t4TACT: IMMEDIATELY FLI)SH WITH LAR-3E AMGIJNTS --'-FWATER F---RAT LEAST TEN i4lt4l)TE-@Ar4C- tiET MEC-1-:AL ATTENTION. I(IN -:----tJTACTf:%EMi@Vr --.:.%tiTAmINATED --.L-@THlING. i.AlirjC-EFStEF---RE WA*-:,H AFFC,-TED SKI&tj 4@REA WITH --OAP ATIC-WATCP. C'r*--:ARC---.--tiTA04rt4ATED ;H--,E.* %PID f(1 7 fi .;r@iF 4ATE,,: a.;fj-,CT MEC-E-:.AL ----------------------------------------------------------------------------- S.P@&LL RESPONSE: OBSERVE PRECAUTIONS FROM OTHER SECTIONS. USE APPROPRIATE ABSORBENT MATERIAL AS NEEDED IN CLEANUP PROCEDURE. COLLECT SPILLED MATERIAL. CLEAN UP RESIDUES WITH WATER. PLACE IN AN APPROVED METAL CONTAINER AND SEAL. RECOMMENDED DISPOSAL: SEND TO 3M CHEMOLITE PLANT - BLDG. 47, OR TO A 3H APPROVED CHEMICAL WASTE DISPOSAL FACILITY FOR DISPOSAL. ENVIRONMENTAL DATA: SEE NON-REGULATED CHX-MICALS' IN 3H WASTE DISPOSAL PROCEDURES MANUAL FOR LASELING/MARKING/PACKAGING/SHIPPING DATA. HAZARDOUS WASTE NUMBER/CODE: NONE --------------------------------------------------------------------------- 7 7. REACTIVITY DATA -------------------------------------------------------------- STA81LITY: STABLE (CONDITIONS ND TO AVOID): INCOMPATIBILITY: zzu (MATERIALS TO AVOID): NO HAZARDOUS POLYMERIZATION: WON-F OCCUR (CONDITIONS TO AVOID): NA HAZARDOUS DECOMPOSITION PRODUCTS: HF. ----------------------------------------------------------------------------- INFORMATION ON THIS DATA SHEET REPRESENTS OUR CURRENT DATA AND BEST JUDGEMENT AS TO THE PROPER USE AND HANDLING OF THIS PRODUCT UNDER NORMAL CONDITIONS. 9DI:11-3 PROuuCw RMSMTICM LUMTMT ZQUIPZW mouth_ 1)-i,4,oahtt- '!ear IRSPZMOX LW Check if Operation Normal Day of Air ft. 3watj&, 31. 1 Gen. Ts IMCI L&T .t,s. Deionizer Lit llepl Da2 --V i,, 4 _ae -4Z Notes 7 20 12 3 4 5 6 .-17 19 -21 22 --23 24 25 26 -27 28 29 30 3!1901 v -to -,e-o' L@- ---& &/ Looo, %.o -.oo, _oo, c- I1-=i __.,oo, w- boo- ool 4oool %oe by/Date !c@(M 17-&- ia-eqr-q& IL-Irj?o 11 4L '?A 12-LI-@RD TIC-, f-si.-rt.o YLail 501to EQUIPUNT CODES TH!S IS A CER'SIRED TRIIF- PY. Air Bi. - Air Blower SiG,4ATURE DATE Gen. - LAT - IVAC - FE - *as. 'T.S. - ELmiegrhgtesncayndGeTniemreartsor 4 Beating, Ventilating and Air Conditioning System Fume Hood Waste Treatment System Daienizer Check "Lit" if both lights are illuminated,and check "Repl" if the unit was replaced Re=Loce Ncyn Wc. SA-@ay d MUFM PRODUCT REGISMTIOK month- I)wlnth4u LUORATORY Eguimw Year INSP=TION LOG Day of Month Pbotoperiod (hrL) Incubator Nuaber 1.3 1,1 cit Light Intensi ty Incubator XwLber 1-4 c3Ft BY/Dat* 2 3 4 5 6 7 910 ,-il12 13 14 ls 16 17 20 21 22_ 23 24 25 26 i728 29 30 31 1I ol-I It.-2-1- -77 -LL- 2:1. Lk--- e*-' 2,L_ 1(. "- -L&- 2d- itil--Z iLL-2*- 1E.-el_- -Lee- JJ.L jk Ik ::g -E&L --@ JAL 2-4- -ifoalit -24 11 L jg 1--l@ 24 to .24 ir@ O'S ---L-qd a- -w -ooll 3 &-s '4-3--r. -L-J.IE -i-s-J,5 44-- g a 12---A-ga /Lt --41., 20 35 L!L 6,% iz-& -11) @16- Jir_ Is -7-1 31. fnl e 1-cf-zol.-3-5- -zi x, I/,- L5- -3595 2-5 -,iAo k5 xaw- @b 191 12--If-.YO J-T- tug- ,::of g:::f 2-7-16 Z-L iL 45- t:::@l 25- 5-5@a-,,-leL @@ I-zr AT- ad- --" ak -3-5-2.2 XAL _va e!:n -JE, .1 -7"@rL -316 -Lieei L2-ii-q- -j4. -LL ga_ it.. 2-IL - ii- Zi- lool 9 la 13 rw tz/!Offv -43 EQU7rMEN-K CODES CR culture room Re&oLiArncceW& ircS-ubs6md KMUAIME PRODUCT ]TECISTRATION UBORATORY EQUIPM13rT C=B&KTION LOG month e t-v Y*ar /4.2dvf> Dissolved ps Conduct- Salinity Refract- Day Oxygen ivity anoter of R*ter: oWt- seter:-L4k.,/ mater:eL-1 metor:_ no.:L" By eo Act pg 4 pz 7 PRIO Theo Act Theo Act Theo IlLct 14 wo-r A ..c 0% 40 09*4 -ads I L/IL7j '7 ii @.3 -Ll-- 2.3 2.0 41.1 101 cl.-4 12- tZ' i 9-C, 1_; zo I g. I a !lj M.OR it.&rj1 7,o 2. &.Af-L far) -1-M lya)- 0414 L44ju')zvfl".& I-do rjoo 1-COV [-/)bo /9 &Ad- lila@ glov zzat If--6#*@- vla-, ?.!r I 't.'g- ism i.,S-; #AL 9k) fir 1.4 1; b-,,5 i-n77 (ion 101 /6-03 /zoo It) firI ay.c; g.6 IL@- 10-05 1 Mf) ;I li -92- Pq 11.4 4.'5 11-3 4 !t Q-i 1&.ffL /,Lwr- -oau -00 ').oi i.ew t.000 4.co 7.c7.3"z&aAi I-CA AO-oi I-ow f-pah fjr ;L#.C 1-~ -.ova 2 t. 19 q.5 J-tL!iALL 1.&v f lIr lei a-im- 1./n 99 3)4,.N Lq -2&t-ig&.,too* 7-OY to 1.5 go* 7-o5 is,#T 9.5 1 -t.-i I +.f.,.-7t.o IJO,,DTI -ZLL Z" AY _2co 2e, &> ad 2,rr Zd 0/1- d 'f- -2LL 26 70 22of . eLl =L^ .71) -,-"I .2d ;rs Z& Theo = theoretical value Act = actual value measured by the instru=ent Thermometer calibration: .Ieasuredvalue (OC) thermometer no.: NBST)4*10tl34 ill@ 1"1 1;15 !ILS ZLII l@k Zit- ZAI-Y' -@;'l temperature 1)!5 ILU 2jC_tiLL 21 LL_J_IL5 _7.L5" temperature 2 L 2121 2146 i6t Re%Xme Atico@ WC. Sts4*tv d W4UPM montb- PRODUCT REGISTRJLTION 71ve-wi6o- 1,,:.BORJLTORYEQUIPXU& Year INSPECTION LOrs wets*ure'and lrel-ard?esp. (OC) Refrijerator Number Day of 010 Incubator Number 42 pc RR 4!$- A* R T y/Date. 15-1 2 A-' ,::f 3 !911-IJiLt.-LL- &-I W- 4 as ,e IOMJA.tAaLJU -3 LLk &- Lf.CW a-S1.2-1*0- 5 &--f L.S- -Z ja A%E. 22.6 -A- LU &I C-5 6 IL ;4 11-7Hf Fn- zz::@ :-A- . 6 124r4j 7 t q lk a-9 &L 2LI 8 3.10 3.3 _g:f -1 15 )Vl 10 1 12 13 14 15 -1617 i9 20 -y22 23 24 ellol*04 2S 26 27 29 29 30 31 -- Lt-2 d 2.z r@.i) L- --- A-,.-To- io2-1 IL P-4- ,L-v A.I AdZ-2- -t6 IQ@ 115" "-a Zia- gu MA aa 11 KO III LL-L r_l aa 2QS P--c ip-D 9AA 127 L2.1 2-t L.L--I 31( 2.lq J.1 @.2.15;.L @--- 1*1-0 MA aL,(-f*tV fq.7 z 2.- aL t- A-Y 1 dL"- --3 -1 2a @E#-O.M A goIt ZLS glid 12-11-17M t6 -qo 17-w-@ -ch., lilleii cv:@I)-t-9-110 Got 15 - -"%414 1!@b CP-2a el 42-20-Vu or6c 3-t Lffewr@ 1&-t ;.5 cfi&-:fj. 72 i 1-11 1 @grel).)L.gto fr,.Atue rv'fwl)ii-40 -&- 012$r-10 ciar 44.t o- $1@/-A-Lftd 1,2-0-74Aftvr 2L.i reLO44. EQUIPN-NT CODES RT - room temperature sample storage area CR - culture room FR - freezer 7H!S A C'--RTIFIEE) TRUE COPY. SiG*4ATURELm^ - DATE-QL05-91 ResojeACreCWt 0--$Anm:bodyMUFM RAI Mr. Timothy Ward Envir*Systens# Incorporated P.O. Box 778Hampton. MR 03842 901?1-3 :icy NJtC38Q i&=- 926,77" P.O. Number: Date Received: Lab Number: Date Reported: 2597 08/29/90 (1320-) 23,394 10/10/90 Attached please find test results for ammonia. total organic carbon, aluminum, arsenic, baron. cadmium, chromium, cobalt, cc%nmro iron. lead, mercury, nickel. silver. zinc. fluoride, total suspended solids# posticidas/PCBIS. Samples were received in acceptable condition, All samples were prepared and analyzed with4- the holding times specified in the referenced methods. x Instrumentation used for analysis was calibrated and/or tuned with the appropriate frequency and to the appropriate specifications required by the referenced methods. Blank cont-=4n&tion was not observed at levels affecting the analytical results. Matrix effects. as monitored by matrix spike recovery. unusual physical properties and/or surrogate analyto recovar3r. were not apparent. Accuracy and precision, as monitored by laboratory control sample analyzes, were within laboratory acceptance limits. RESOURCE ANALYSTS. INCORPORATED TWISISAGERI TRUECON. SICNA7M-&h-MTE-elVqo Authorized Signatur; It= . Date FiOld Identification: 2597-7 LaboratoL-S-.Number-. 22"4-7 Parameter Total Suspended Solids (mg/L) Concentration CIO Matrix: Water Date Analyzed 08/30/90 Method/Itef. 160.2/1 Field Identification: 2597-8 Laboratory Number:, 23394-8 Parameter Amonia (mg/L an N) Concentration CO.2 Matrix:" Water Date Jknalyzed 09/04/90 M@thod/Ref, 350.2/1 Field Identification: 2597-9 Laboratory Number: 23394-9 Parameter Total Organic Carbon (no/L) Concentration Cl.0 Matrix: Water Date Analyzed 08/30/90 Method[Ref. 415.1/1 Field Identification: 2597-10 Laboratory Number: 23394-10 Parameter Uumiw2-, total (no/L) hrsenic, total (mg/L) scwon, total (mg/L) cadmium, total (mg/L) --hromium, total (mg/L) cobalt, total (no/L) copper, total (mg/L) tron, total (mg/L) Lead, total tag/L) Mercury, total (mg/L) Nickel total (mg/L) silver, total (mg/L) Zinc, total (mg/L) Concentration 0.04 CO.002 0.1 0'.0005 <0.602 <0.008 <0.005 0.03 0.003 0.0002 <0.008 <0.0005 <0.005 Matrix: Water Date Analyzed 09/07/90 09/03/90 09/07/90 09/29/90 09/07/90 09/07/90 09/07/90 09/07/90 09/03/90 08/31/90 09/07/90 09/28/90 09/07/90 Method/Ref. 3010,6010/2 7060/2 3010,6010/2 3010,6010/2 3010,6010/2 3010,6010/2 3010,6010/2 3010,6010/2 3020,7421/2 7470/2 3010,6010/2 3010,6010/2 3010,6010/2 Remuc* Ar4%V& ft. $AnC-ay d %%I.VCCE Field identification: 2597-= Laboratory* Number: 23394-11 Parameter Fluoride (mg/L) Concentration 0.3:0 Matrix: Water Date Analyzed 08/30/90 Method/Ref. 340.2/2 Field Identification: 2597-14 Laboratory Number: 23394-14 - Parameter Total Suspended Solids (mg/L) Concentration CIO Matrix: Water Date JLnalyzed 08/30/90 Method/Ref. 160.2/1 ri-'Id -XA&mtif ic&tionz-- 2597-3.5 Laboratory Number: 23394-15 Parameter A=onia (ma/L as N) Concentration <0.2 matrix: water Date Analyzed 09/04/90 Method/Ref. 350.2/1 Field Identification: 2597-16 Laboratory Number: 23394-16 Parameter Total Organic Carbon (mg/L) Concentration C2.0 Matrix: Water Date Analyzed 08/30/90 Method/Refe 415.1/1 DemmeAECW&VC.&AmotloMvUd.VCM Labor.atory.,-ni 23394 -012 Sample Designation., 2597-12 Date Analyzed: matrix: 09/08/90 WATER PES-TICIDES/PCBIS CONCEMERMON DErtmom L= ALDRni ALPIELA-BHC BETA-BEC GAMH)L-BHC DIMNM-IMC @ CHLOPJNM 4,4*-DDT 4 41-DDE 41141-DDD DIEMR331 ENDOSULTAN I ENDOSULPAN 11 ENDOSULPAN SULFATE ENDRni MWRIN ALDERME REPTACHLOR HEPTACMIOR EPOXIDE PCB-1242 PCB-1254 PC]3-1221 PCB-1232 PCB-1248 PCB-1260 PCB-1016 TOXAPHENE ENDRIN XETONE MLITHOXYCHWR BDL BM BDL BDL EM BDL BDL BM BDL BI)L BDL BDL BDL BDL BM BDL BDL BM BDL BDL BDL BDL BDL BDL BDL BDL BOL 0.06 0.06 0.06 0.06 0.06 .6 .1 0.06 .1 0.06 0.06 .1 .1 0.06 .1 0.06 0.06 .6 .6 .6 .6 6 :6 .6 2 .1 .6 METHOD REFERENCE: 40 CPR PAW 136# FRIDAY, OCTOBER 26, 1984 IEL"ZMD 608 BDL Below detection limit Laboratory Number: Sample Designation: Oate ]Cxtracted: Date,-Analyzed: Matrix: 23394-13 2397-13 09/3LI/90 .-10/04/90 WATZ3L PESTICIDES AziNPROB METRYL--BOLSTA]T CRLORPYRXFOS COUMAPHOS DEMSTON-0 DMUMN-S DTJLZINOR DZCHWRVOS DISULPOTON STROPROP FZNSULPI=xoN FENTRION MERPROS MEVINPROS NALED PARATRION METRYL PRORATE RONNEL STIROPHOS (TETRACHLORVINPROS) TOKUTRION (PROTEXOPOS) TRICHWRONATE CONCENTRATION (ug/L): BDL am BI)L RDL BDL BM Bm sm am 31)L BDL BDL BDL BDL BDL RDL BDL-BDL BDL RDL BDL BDL - BELOW DETECTION L3]11T METHOD REFERENCE*. US ZPJL-UMOD 8140. DETZ=OK lon[rt (UGIL) 1.5 0.23 0.3 1.3 0.25 0.2S 0.6 0.1 0.20 0.25 1.5 0.10 0.2S 0.3 0.1 0.03 0.15 0.3 5.0 0.5 O.IS Resauave laalvtxo IDC I hm1i;&@tms ft"uct RegistL-atim uumtmx CEM or CVSTM DITWCIA 'Studl Nusucr: Date SS;aU"mp1o1lp ed: OL-7 to Notes: 5,ce sample' Identification Stuft man*: Fk Sampled itanimram size of containerit Remarks Sample Li PLO$ k 51-1 we-ti I ;5--,Lit-ss TOC -10 :P"- $-*k )0 -(I -fcacplv&9. 1) FIL @l&ss , 12- 0 . r .5 Relitguisbed by Relinquished bY ktt-o Date "me 13>,D Tine Received ke Receive _AX Date Time Date Ting. TRUECOPY. SIGNATURE gt:@5 DATE-0@1-0 PRODUCT Unsm7m umn?03ty sa Lt Rzcnmc LW 1. M Sample Number 2. Sponsor Name and Address P& 3. Sponsor Sample Identification 4. Sample Container Label Data t36' -B.,sL A-ic. IA tJ 14 2it,4-c P /A j@po F S. Date Received- it-Ls@so 6. Date Photographed 7. Method of Shipment Shipped From MA) via oceifo&@X S. Description of Shipping Container(s) (Number, Type. Size) f4, 4- 1. Description of Sample Containers(s) (Number, Type, Size) ASO V"t 'su4s, 601@-tt 10. Sample Storage Location and Required Storage Conditions-.FA( -If- a,.n-4-P 11. Signature 12. Notes 13. Date and Location of Arcbive Sample Removal and Storage Location -Ifo 00--f,-5' THIS IS A CERTIMED TRUE COPY. SIGNATURE 5*kn DATE_ON S c,1 ft3opce Arx:ovft vc. Sts-ocro, cl *.V.LPOC-C . .. . . . . PU fz MISISArA31TVIEDTRUE COPY. SIGNATURE'12@gL6kE@@Icit Lai Pctnt-A TP.S:l@ A CE.RTiF:iED ;lr3o%ATLIRE@w.1 DATBZ328qi