Document oe5MnadQjx5orz7Xya3ZjjNXD

AR 296 - 0908 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: tMoixatsurPeFcOoSn,taFinCi-n9g5,PeorrfalusoarococotmapnoensuelnftonoaftFe;C-m30a7y2.als(o1-be referred Octanesulfonic acid) (CAS# 2795-39-3). Remarks: is FC-3072. The 3M production lot number was not noted. Current information indicates it is a mixture of The test sample 1.67% test s5u%bsstoadncieu,m 3oc3t.y3l3s%ulfdaiteet,hy3l.e3n3e%glsyucloflonbauttyeldeatlhkeyrl,e2t9he.r23su%lfwaatteersa,lt2s0,.48.36%0%urea, acrylic foamer, 1.67% triethanolamine, and 0.33% tolyl triazole. tTehset sfoulblsotwainncgesiunmwmaatrerysaoplpultiieosntwoitthheitnecsotmspalemtpelleyacshaaramcitxetruirzeeodf the concentrations of impurities. Data may not accurately relate toxicity of the test sample with that of the fluorochemical component. METHOD Method: OECD Guideline 209 GLP: No Year Completed: 1995 Analytical monitoring: Dissolved oxygen, temperature and pH. Statistical inhibition in methods: respiration Resulls rate. were determined by calculation of the % Test organism source: Activated sludge mixed liquor collected from the Metro Waste Treatment Plant, St. Paul, MN. Test condition: Dilution water: Milipore Mili-QTM water Synthetic sewage: per OECD method Rreeffeerreenncecesuabnsdtatnecset,s3o,l5u-tdiiocnhlporreoppahreantoli,onw:asA psrteopcakrseodlubtyiodnisosfotlhveing 500 mg in 10mL 1N NaOH, diluted to 30mL with Milipore Mili-QTM water, then brought to the point of incipient precipitation with 1N H;SOj awnads dmielautseudrteod1fLo wbieth7.M0i.llTie-sQtTM water. The pH substance was of the added reference solution directly to the test vessels. Test vessels: Not given sNuubmsbtaenrceof concentrations: five plus blank control and 1 reference Temperature: 20.7 --21.7 C Total suspended solids and final: 1.6 g/L), final pH 7.0 pH on day of testing: 4.0 g/L (TSS in Element Basis: Respiration inhibition as determined by oxygen consumption. 002948 Remarks: Uptake rates corrected to 20C for calculations. RESULTS Nominal concentrations: 2 Blank controls, 100, 180, 320, 560, and 1000 mg/L. Reference substance at 10 mg/L. Element values: 30-minute EC50 = >1000 mg/L 3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the fluorochemical proportion.alone. CONCLUSIONS The test substance 3-hour ECso for activated sludge respiration inhibition was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number M3597, 1995. OTHER Last changed: 6/25/00 002949 BEST COPY AVAILABLE ENVIROAMENTAL LABORATORY ACTSILUDVGEAMIXTED LEGUDOR Bm ahs Type test JECD Achudel Thode Keuueidis Tabb tos Teteooq ACTSI LUDVGEA MIXT EDLE IQUDOR owe Ovaines 2a fis bate Uses 2b fo tied Liauor Suspended Solids (MLSS C_ gi Adjusted to = gi. MLSS in final test solution __| gu Cale, renatid xe 200m sme et ital pt NVA (1st test soln. prepared) Final pH _7.( (last soln. prepared) comments Talbicl oflwus wot dabin ovon 2 TESTSUBSTANCE , Bothy lene glyect = sample Description FC= 2012 AFFE Rare (Cob wvosibbdoll, flay? Tiiare Iota pH of highest conc. tested Lucca) = (Co AdjusttoepdH Comments --- BEFERENCE SUBSTANCE sample Doscrpton S 3,5 DCi PRelul cnmce ber Initial pH of stock solution (L(Y mg/l) = 7.0 Adjusted to pH _----_ Comments EXPOSURECONDITIONS: 5.3 Jeifrs Test Duration _~SCrams /3hes fe Test Temperawre Range: 30,2=31.7 C Comments splas Farid wp comida dase i hendec | i petal 2 } Lcoonptns E AEsREER aliSeBe 1 HI es E ot CEE HU 002850 BEST COPY AVAILABLE geet? ws 97 Tost Sustance: _ FC -5072 AFFF Bago samp Doscrpron: Cereb. Die toglene alycel fenclobil othr LE Ansys (0D Durle Buber Hyd 72 Oate: --3fecfas Joa Gof), ey W. i Am mee.n, E S E T SUR T JOR HI seme TI S--ye---- Ja--c--r ro --- ------------------ Tirn s I Te -- or SEE pr Caen a BRGEE -- ---- ld Ha CrGeagy ENGR La es= momma CL ) Mitporo MOR high purty ve i5 : berg HRI 0 SERRE ig0RYEY BEST COPY AVAILABLE ENVIRONMENTAL LABORATORY RANTT RAW DATA (OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 I Test Swsiance: _= CZ ZCT7) AFEE Rag Source of Activated Siudge Mixed Liquor: Oute Received: _/_/72~ Avast (no icfeotia to tutor Ten: Date Uses: EERE Li = EL] 30-MINUTE EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS, mgt. CToenscwanvaatino(n+.J-) 1s 2T5R3EM.N0O]TES35 <5 Temp SN --_-- z= TY ZoG -- ea abel bello ISP FR IIE LreolleaalesTe ra KEW S 1Ds 1 I Eelrar== r oorr alr > cel TT rr cel rrr Try 3X EYES CYYYIE FE A Eo lealeolcslealuqlaclnalzelosle (a7laon HOUR EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS, mgt. TestSubstance Concentration (ujL| 1.0 TIME - MINUTES 25 [3.0] 35 Temp c ea - = . z IonT) IeseolIsNeTlslaaemTaizT3s9g1l5aCI5 voTaoa[b9a7[i7a R [a1ez ie [s21S<T82 fda [NeTur3a 3C3210 8[0a (21) So JS) [$3fTalqi]edPaesteepoIr.nSHEa6[7u.y75.9T1h =] 21.1 RE r Asdlshlur gleaT lwe 5- 65r 2hT s seeTrT PBHH ooffaHcoinveasttedcosniudcge emymainxonedestaiuso;r:" _ J Iniotial _i 2otpia 7oAeJ1A25f57h0)rFminaal GaTaro 3hs hs Test temperature: 20.7 d 17C. CEL a wo I e:ba SSFiYEEmReEVSTSEEER i Sa RR ie5 > 5 he< h Cl e= HR NE bee bee Saal ip 00295R uy 3 ounTouTemua ssoouaToPne:san TENTST 2) ouvh Saas +HOS5F8: 3 To Str: ECATAEBASE g g sms % 4] = 2 85x soins xgone GustOsmen Gress, ot. won| apaTu0sfaTerfo[eeErsfTTpe7ro5]o30p[5uspsa4r0op]s4eprsT6aspr05peesie[pnsemaep[oAmrME [oaMtpE EaEs] + =|| 7# 8 2B5H EB Eatre Eo e R HsH A er 1 eesHe se ii1eo1sT T: a7m3sa T7 osae e]|| : i: p o rteemrto toe rtetorLteise t on > 16e 26 ase aeTeo2rT7omr enf7eesemmw--e|Sl| J Gusts One corvmni, el Be pres oprEpEopar]sofosjtofssfSofisfans |AOPRA 2 P F Ee e er e IH E H E e HEH HSH eheerm erTHetTTaoaesTooaTowsoeoss3TTiCaamsmla tmieintea|os||]vi 5ixF F pE refere perE ieeetepartetpSeaetroor 6at7 met(e6l0oi eatTorsn lt estp eorrTPot Em RTa ooEnmtat] : si mane, BEeT t nedmer A Het SshRWFs nd a BEST COPY AVAILABLE n4o Tek? ENVIRNOMENTAL LABORATORY HE wa 3397 0ECO ACTNATTEEDSTSLSUODLGUETIROENSSPIPRRAETPIAORNATIIHOINITION TEST #209 rest sumwanees 2 T= Do bloeo stenel Selenace samplo Dasarption: _L3 oed 1 Lala l P3 VA1 (by Chy imie (0 pow ICT oa re jqs : Sot fe Lor?a Covi Bremen Lisi ied COP fal od IN i Ea ye er T n e m CEE THea TSoolG"o amwbsadmtaedl mbmMOoU)owtater fmLessyne.wsasva0gen esmuemnecaion TootoVele Sesfedo awwoC ace con TT TT Trr T T T T r I TT OGrE =e TT Ty TT NFfL -- IT i AL te Be LN 0) Wilipors MGR high purity water TR Bai "Eh LT fy FE ee n ie oppes4 HE BEST COPY AVAILABLE Te Gel7 ENVIRONMENTAL LABORATORY LRIM3392 ECO ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #203 RAW DATA Test Substance: wc Mors shoo Source of Activated Suge Mixed Liquor: fideo Unbe Vivek eT dun bib tii, ousRaceve: J: [i oate Uses: "J: fim 30-MINUTE EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS, mg. TConecenrtrmatiao(n. 2TR5IE3M.RO0TE3S5 Teomcp = eo bd 8 o bab dl-- _ Calezlealerlealzelog[vo[0 Id -- rre rrr e rT NIANAeI e A I Kao C : r y 1 TS HOUR EXPOSURE DISSOLVEDOXYGEN CONCENTRATIONS, mot. Tes Scbaance TIME - MINUTES Temp Concentration (yc) 15 2.5 |3.0| 3.5 oc Ee sds[4alelaxa: 5l20s]9s7] 20 " & [Zz T7ole eleCeCeqlIeaTe [59 [5% MH--------1+--F1++ YI IN NN A NN A 1 -_ mm--mSm--meety > - 1 == Fr 300ETAEESFV EY EX EYPe ET) Roofacitivsated sludge mixed liquor: Initial I OooF wae fLiFEn i,nan aeor3 Fest temperature: 20.3 = 21.4C Sra apg oS St 002955 wy | cCumRoweETnesTvAemcEetoer R TONTETvm owe iSaimnz 82 To ner 250ACHREIL aHEKi 2sS| spee so on good eala 5a S| ASSE eS e amS ares, | 58e8 | F E hptmeeeeptroel teuptoeraseptveeapre traersetosaeiteaupvartre eaaaostaonnteeoTpootswms osfefmeottmaa--ml]]] 3: & 8 e 1 Pe s pp ss er| t raete tA otter restoma E mmmE ms] E E r TE eee ee ee aeeaee]) [ F F Ep Ese pF ee efe eF tees rsfeSaeeEe eraAepnAepteOTe fiese t Jomomn fe ei mnme | Et en es | e e e Loreease r any mal s wrfeomm soa --ow]e | ||| ne eens, ro % 3 uy acon 3| tet | me026e 6g3 p 2 5 fC EE-- BR 12% Hem a re cf (Ovrellaiial i ------ @a [C OoE mmpene|he Tali FE h -- ---- 1. Ee] TTT TE Hr Co T A e Rr r EH ee [od -- EA A | EE MESES -------- ---- [me Joop | foe pu,L|LSmO et| postt ition|T] e| pos ES -- oT IT eT 7 Ly TTT