Document oe24rrJMO01y3azXk65GbxdvE

AR 226 - 09ol ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity: rAefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f9i5u,oroorocatsanaescuolmfponoanteen,twohficFhC-m3a0y41a.ls(o1b-e Octanesulfonic acid) (CAS # 2795-39-3). R3e04m1ar(kcso:809-1T)h.e C3uMrrpernotdiuncftoiromnatloitonnuimndbiecratwesasit2is30a0.mixTthureeotefst6s%amPpFlOeSi,s FC36% doicteytlhyslulefnaeteg,ly0c.ol55b%utyslodetihuemr,la3u6ry.l0s5u%lfwaattee,ra,n1d20%.4S%ulttoolynietrfioaazomleer., 9% sodium The following summary applies to a mixture with incompletely tchhaertaocxtiecritiyzeodf ctohnecfelnutorraotcihoenmsiocfalimcpoumriptoinese.ntDoafttahmeatyesntostaamcpcluer.atelyreflect METHOD: Method: Not given. Type: Acute static YGeLaPr:coNmopleted: 1981 SSuppepcliieesr:: PCiommempehraclieaslpsruopmpleilears, Brady, NE. AEnxaploystiucrael pmeorniiotdo:ri9n6g-:hoTuermsperature, pH, and DO. Statistical methods: LCs values calculated by moving average angle method. Test fish age: Juveniles Length and weight: AAvveerraaggee wleeniggthht== 401.4m9mg (wet) PLroeatdrienagt:me0n.t6:1 gN/oLne Test coDnidliuttiioonnsw:ater: Carbon-fitered well water, St. Paul, MN DilutioHnawradtneerssc:hemistr2y5:3 mg/L as CaCOs Alkalinity: ~~ 221 mg/L as CaCOs Lighting: Not given. Stock and test solution preparation: The test material was addeddirectlyto Cthoencdeilnuttrioantwiaotnesrdoonsainwgeirgahtet:/vOonlucmee. basis. - EStxapboilsiutryoefvtehsesteless:t cAhlle-mgilcasasltsaonlkust(io3n5sx:20Nxo2t5 cnomt)edc.ontaining 16 Loftest Nsoulumtbioenrs.of replicates: 2 NNuummbbeerr ooff fciosnhcepnetrrraetpiloincsat:e:fiv2e0.plus a blank control 002898 Water chemistry during the study: pH range (0-96 hours): 7.9~ 8.1 (control exposure) Temper7a.t8u8re.1ra(n3g2e0 mg/L (0-96 ehoxupross)u:re)1"8-19 C Dissolved oxygen range (0-96 hours): 5.0-9.2 mg/L (control exposure) +320 0.29.2 mg/L (320 mg/L exposure)* mg/L (second highest concentration) exposure values used because the highest concentration used resulted in total mortality by 48 hours. RESULTS Nominal concentrations: Blank control, 87, 135, 210, 320, and 490 mg/L Element values: 96-hour LCso = 240 (217 -- 265) mg/L. Element values based on nominal concentrations Remarks: `Substance Testing was conducted on a mixture Remarks field. The values reported as described apply to that in the Test mixture and not the fluorochemical proportion alone. CONCLUSIONS Tmhg/eLtewsitthsaam9pl5e%9c6o-nhfoiudrenLcCesoinftoerrvfaaltohfea2d17mitnon2o6w5 was determined mg/L. to be 240 Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 2. Testing meets the criteria for quality testing. Hanoawleytviecra,l csoanmfpilrmeaptuirointoyfwtahse naomtopurnotpoerflfylcuhoarroacchteemriiczaeld parnodpotrhteiosntuindythleacskolsution. REFERENCES "This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 6508S, 1981. OTHER ------------------ ---- Last changed: 6/26/00 002899 NiRrFe UL aeang roe _ASOAS roeTest ut,Aleavoters esos ma a rest. organisn AAZAEK MUMSreTRAY,MESDate Recta JAS] Baten 8 Z=f5/ wg.we.J,4 gras Avg. Size 4,/ om No. Organisms perdose 2()loading Factor (J. lp) _ 9/ a Disasse Teeatmmt:_N/E Test Solution Volume//sLiters/All-Glass Tank TankDimensions: 35x20cmand25 cm deep rine started /// 3) fy pote started ZX [9/8] Date Canpleted_(, [5/6] =I Ear Ere EE PEE TE Dilution Water Carbon-filtered well water (Temp. / _C., D.0._Z. ZL pm, w_&, [ ) estator fF TITS tog GR,RL (Full Signat J... 8.\Cs0 240ml! (1/7- bSHgt) -- aA Me es So ze 1zs ln]0 Rdsd 2, Bans]2) bbl] <7 bedig arl=zolsizol 4 RaeS INBoal(Abdodl14 bA sel1xa Rdq ed2o 0 Gal na 2e 0Fa ET2] 0H e J m L71 00 ] | T oTrT| Jo1r 1|1 10] itialsTate A atilalain Lindl 123ZI Omench Sows 2[NOW DUR GTEC IDENHTS, AT AMRENT RNA TrayWATFRIAL |S TE6 WDPAERRKATAUMRECE Foi Lil Secondary Effects (other than Mortality): ECs0_ -- 2 [orp o moins| i _ No. | symportReoactmiosns 24H. 48-Rr. 72-He. 96-Hr. eet Awe |Aves [Moe | Ao wll Afr|Avsar |Mwe | Aras 261 Ta Td Tho 1 She =R] Tan PAu Aa TT Aha RBT T BEST COPY TaAVATnAG LPAe s TAG T-- X DRPINITIVE (FOLL-SCALETEST) wR (aSORS ype Testur, XurESynucraterial Test2ZeKd) [LEOPAL Jot2300 Test. gents bTAEADMIMI oesTorr,Ms.votevecta LASJ) match v2. 1 ge ), 4/9. grams Avg. ria pisesse Treatments size4|,om _AGlie to. Organtems per dose 2() Loading Factor_(J.(p/ : Test SolutionVolume /( Liters/M1-Glass Tank Tank Dimensions: 35x20 om and 25 cm deep =I rer Pere Fre PRE ptlution Vater Carbon filtered well vater (erp. /8_C.. 0.0. G2 com bBo] otome TLTEPS TFS EaENF = THO IHL a - used MO iy A Bi ANG METHO0 . AD (me | 777 770 1770 -- pa Act= 2) kz dsao l 7lds oa i 0Vz AoAo [| l FA) sc[racked 17 kadiA20 bavd zo FF zo H 1 1 TT 111 J 1] init&i Daa tels ----J=9=3" 7787 =m 7p31 Fm AT2S JT EET IOWADAL TTEICITS Test AMOERINL |S A DORI RIETD. TORY LIC Secondary Effects (other than Mortality): El a hmrt EaC50_ lal. ---- oodt | fMae |Ape|Apa | Aton. 21 Ed I EoI 1d NVXa |Ap | Ais YEE W/V 7S VV | Joe | lis] PA BEST COPY AVAILABLE ~oozs01-