Document oe0qENE2mKzG4g1bRwjEmbMmo
AR 226 - 087/
MICROBICS MICROTOX TOXICITY TEST TESTSUBSTANCE
Identity:
rMeifxetrurreed
ctoonatasinPiFnOgSp,erFfCl-u9o5r,ooocrtaansesaulcfoomnpaotenewnhticohf
mFCa-y30a1l7so.
be
(1-
Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: is FC-3017.
CTuhrere3ntMipnrfoodrumcattiioonn
ilontdincuamtebserFCw-a3s01n7otisnoatemdi.xtuTrheeotfe0st.6sa7m%ple
P1F.3O3S%, H2y0d%rodxiyetfhoyalmeenre,gl1y%coslobduityulmeotchteyrl, s5u5lf.a4te1,%0w.a5te%r,po2l1yo%xyeetthhyylleenneeglycol,
monooctylphenyl ether, 0.04% sodium lauryl sulfate, and 0.05% tolyltraizole.
The following summary applies to a mixture with incompletely crhefalreaccttteorxiizceidtycoofnctehnetfrlautoirooncshoemfiicmaplurciotimepso.nDeanttaofmtahyentoetstacscaumrpaltee.ly
mgmwoR.
Method: Microbics Microtox BASIC" Procedure
Test type: Acute static
YGeLaPr: CNoompleted: 1995
Species: Photobacterium phosphoreum
AEnxaploystiucrael Statistical
pmeorniiotdo:ri3n0g-:mipnHutaensd osmotic condition. methods: EC50 values calculated by statistical
linear
regression
TpersotgroarmgparnoivsimdesdofuorrcMei:crMoitcoroxbics' Reagent.
Test coDnidliuteinot:ns:Millipore Milli-Q" water
TsSoetlsoutctiktoenamnwpdearstaetpsurtresepo:alrueNtdoitobnynopdtriesedsp.oalrvaitnigon2:00Am2g00of0tmesgt/sLupbrsitmaanrcyesitnoc1k00
2mnLdoafnMiolslmiopotriecMaidljliu-sQt"mewnattewr.asTmheadpeHuosfitnghe2s0t0ocmkgsoNlauCtilondiwsassol6v.e0d, in
10 mL stock solution. Appearance of test solutions was noted as
"clear". All test solutions were made by proportional dilutions.
.
Exposure vessels: 4 mL cuvettes. Number of replicates: two
_
ENluemmbeenrt obfasciosn:cePnetrrcaetnitonlsig:ht Floosusr plus a blank control
RESULTS
Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L
002787
Element value:
155--mmiinnuuttee
ECeo ECs
= =
>1000 mg/L 785 (728 -- 846)
mg/L
30-minute ECsp = 558 (541 - 574) mg/L
Element values based on nominal concentrations.
SRuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTduhcetveadlounestrheepmoirxtteudraepapslydetosctrhiabtemdixintutrheeaTnedstnot the fluorochemical proportion alone. conclusions
dTehteertemsitnseudbtsotabnec5e5185m-mgi/nLutweitEhCaso95fo%r Pchoontfoibdaenccteeriinutemrvpahloosfp5h4o1retoum57w4as
mglL.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133
patAQUALTY
Reliability: Kiimisch ranking = 2. Testing meets all criteria for quality testing.
However, sample purity was not properly characterized and it lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES
This study was conducted by the 3M Environmental Laboratory, St. Paul, MN,
Lab Request number N3188, 1995.
ower
00000000
Last changed: 6/26/00.
002788
BEST COPY AVAILABLE
fh-hic
:
ENVIRONMENTAL LABORATORY: MICROTOX PREP SHEET
LR: _y C3)
AnDaaten: 1L/e2? [47
TEST SUBSTANCE:
-
[e-7ein
1. Preparation of Stock Solution
*FMiansasVDiollutTieosntVSoublsutmaen.ce: ca2y1lv
* Diluent:
ERAN
Final Concenatonof Sock Solution: 2s IL.
2. pH AdjusmentofStock Solution
*DIpnHiiiaAAlddjpuuHsstoiedfdSw0tiotchkSolution: --__--_S&_C'
|
3. Osmotic Adjustment of Stock Solution
*Osmotic Adjuster:
4a ll
Comments: did (old Fllohool celilis fo Cos Hill.
|
app ad Osmo Stn
(aueigor oPague erctens)
oor: _----
* Comments:
?
.
5. Comments
Sai toon Te
2
--
En
Lo
SEER
a .
}ie
os gi am a e 002s 789
BEST COPY AVAILABLE
oY ele
FELE NAME. Nila ES5
HISKOTOR DATA REPORT TFEESTT TiATnEe afaAlan
arIe:EBASLrEaion oo1090 ma
TT orion Ea
-
Hina
enn
s i3S .h13e7 e 855a.5le0e
: :
i5s3i.s3i15d)s7
kaurlea 43.03
Maha
Con
115522058.. o0003n0s S5o0 nann 15F0e0 dsaon
oa,
iyWi ae on
_--
assez.
;
3
1
:
3
JET
3
or
soso
3
10. 1@0000uiTB0LLDDDDDDDD1D0"00D. D"ECDODNDCDENDTDRDADTDIODND.DD1D0DE4DOUBDODDDDIDLOIPOD1Y0.5
.
EcH01304.9501
(55% CONFIDENCE BANGE:1116.0963 Tu 125.7596)
Shh
*
et
Ch Used for ealeulations
|
bo 2 ERBe A
vp wanal
ag
i ph
002790
BEST COPY AVAILABLE
STATISTICAL FERURT FOF DATA FILE Nita .
NOWIER OF DATA Wh.B4 SEED
Tren
Ct
eer SETRARVLATED BED
oe
;
1 gin
i
5b
sh Se SGa OREO
A
ay,
BEST COPY AVAILABLE
A TAp n
a
Wa
oa
i
0
al iaze Glieas isliss
a
({[Touch SPACE BAP *, wntinue
SUCCCCCCOCrLOLerorrreericrreeeeeeel,
-- ERROR RE
ra
:
hier AER oy oy PORPIX
BEST COPY AVAILABLE
bane Co
WETOx EAT RR
FL NAW: Miia:s EIS
rTeeaorr Toaites SEoHan
fa
fs
eh
.
;
R aHaR SE
fr iFhensie. sosLonnoon
Sis on W vvi ioT
:I
.5
53
3P 3.0oi i
:ees
333 3
10S 15O0ULODDIOLORODD,10D0*0P.OOGDODNDGDEDNDTDRDADTDIBODNP.AD1D0"84DODDDIDYEDDIDTIPYI1Y1,4
RCO 784.8150
(95% CONFIDENCE RANGE: 728.4058 TO 845.5927) 3
# vad for careutavions
i
EL
Bele CERr ngy e 4 :
spa
as 002793
BEST COPY AVAILABLE pyCefre
by
STATISTICAL REPORT FOR DATA FILE N3180.K15
:
: ESTIMATING EQUATION: LOG C = 0.9025 x LOG b +2.8948
i NONBER OFDATA PAIRS USED: 6
1#577)on
RESTDUAL VARIANCE: 0.00050
55% CONFIDENCE FACTOR: 1.07744
FOR ECSO
`CORRELATION COEFFICIENT: R = 0.99724 -
Ji "CORFFICIENT OF DETERMINATION: Rz = 0.38450
J{' JEFFECTIVE CONCENTRATIONS (95% CONFIDENCE RANGE)
3 +gIhre
ha 3 3
i i
EBEBGCGoGs1000
:
721820448...860103548079 1274081
mmmggg///LLL be/L
((( 2702S38L...042007358855 TTTOOO (814348 TO
821442587...55890724973T)))eeexxx 15.2365)kas
i
aA CEdeene a mainn korepsu
ii Li LiHFe
eodeSoa Wada
Age
EE
WR LG
Ba ema E
cd
LeFp pTR a Soalk
EERE
Gledigen
ih
Jui SR
} Co CLpeieeR ntE s EN GE ER BEIRRSRR A TL Loe
TSARse EO E c e a Ee
Re
ee
ERT SE Se
Ax
BEST COPY AVAILABLE
Fy7Teftio
SoH INIOTNIDAELRDATA CONCENTRATION GaY rin X ENF3F1E8C8T.K15 pi
i z1 i5
121525..00000000 250.0000
00..101582 01236;
120a2.1l50a35
5.50
AHIE
a i
50303000...000000000000 Ob0o3oi88ee0iE1hr 1 33377107612020
2.21 8
GE o RBFiEGnE
1100000.00000000
1717420385
:
TJo
i5sl28
i
56.08
AE
AA
5
{cL zouch SPACE BAR to continue... #i A, LOCCELCELCOCOCTOCICOIrEOCrerrecet
$0 SeINaL RESULTS
Lo ASASr sree Kas
ER x GP H Er CoCOc NC. R | GAMMAs . s |CAUNA/ t H ESTAEIe X (FRs EECT pSis
G 2 C Bich:o2s5o070o0o0n0 3 0o72l7e7s0 SRXKo0mFnonia TgREuEsaIEeR0d0Te\S iE HLoE
k& 3
kDBRas do2o0i0o1o0t0o0i02 o0i.5s9o2os3 ba 1000 00005,0 12352
SiHEiEnR keAoLl: BRE(ASi)R.197bgaisdEi T CENR oaR aREST SHE
T Ls E Fay UE
B00
I
FE a AEROS
oea aEope
RE BRET
LH
ae
ONETDENGEGANCERRERR Ia Je 45027) ak
Z
B L U n el
Radia os ole Cg
i
Hee
PE 1
NT
TT
Le
002795
BEST COPY AVAILABLE
pg 8:10
}
HICROTOX DATA REFORT
FILE NAME: {N3188.K30
eBLrootvmeiesdtuirgfeaerttoerrB:iAnBGtIiCoOn:T FE-a017
AoTTpeEroSorTvedToiwbneey::mSi/aercofasm Osmotic Adjustment: NaCl
TFoEsTtetaHlinSeSoronnecSsoentmrian"t0niioen,1:0/11000 ng/L conc.DCioioneeStsinoenratFGiaaocntnorUnite2: m/l
1 ai86.18/,A1159
125.0000. + 0,16644
hs
:
2HE778aR a5ni8n5ae7T 4s22e81m1me5 He
130320005.::.0000000000000000, | 30: 0000
0000:..313a340e0777824500d00e8 0.541808
#
CoNTROLwa1Sra/i7o0se== in85sm 4*04'/4aei 9nn51a.e42
52.5211,369009,:200.00700208 en
12a011300018e
CoRECTION $ACTOR = 0.3632 10. ZoUDUDDAPEODDBE RIpDRDYDD
EDNSDDDDDDDLDDDDDIDDODDDEDPD?
SHSoLaOPa E =;C 1692
TABIRESaes
Ce sas
3 dat 3 0p
RIE FAIL
2GaeGA 0 anSER WE CE a adn
ss
Ei 3
A SaER er sae
>
S paEtk RoRii s
gr:
ERESh a ETT Rd e Ste e esAHh EBha aEpRh RSAERe IAR TKE ,T3 E dig
Hen SOC ely
a oa is
VTARek
9 E Sn y
ooBet ath o AEG
3i
a ERR
EY)CE
002796
i
BEST COPY AVAILABLE
73? ofl
STATISTICAL REPORT FOR DATA FILS N3188.K3D
ESTIMATING EQUATION:
wxess oF DATA PATRS
LOG C
USED:
=
0.8541
x LOG b
;+2.7464
:
;
RESIDUAL VARIANCE: 0.00012
45% CONFIDENCE FACTOR: 1.02998.
FOR 050
i
CORRELATION COEEFICIENT: R = 0.99932 -
CoRgEICIENT OF DETERMINATION: R2 = 0.99864
EFFEGEE CoTsICVER + C5hO5N7e.C7EsR0N8sT7RAA TmmIeOelNS (9I((53%413C.O44N77F6I62DENRTTCOoE HRSTATaNAiG.iE4vg)2i:iAhTbi)eerse 7 EES (NER Ram
es" EXTRAPOLATED, RESULTS
wa
Ce
Te
2
5
#i
J SabA
oSfh Ee
Fa
Fen
RR fo
aot
SSSEEIRERAh eue m
Rk Te i SE CRS
fa
Se
ee
FRYE EE
Ae
Set hee cae
Ts van
Se
1
002797
910661
a;
BE!ST COPY AVAILABLE
tTIAL DATA
NOHBER CONCENTRATION Ch
]i
i:5
121252505.--000000000000
000.1:1456178
i
:i: +2 203550000:..000000000000 000..0558198s7
:$
11000000:0:0000000<1; + 21a:e7s8
eye3r1A o8r0 30A, " 112030.824%7 13.55; 2Bi0s4se0 25:.04s2 Loo5e17 66.76
4
5h dd
i
iy
Fo hn Ln fe
Go Sn Aelita ASY
(Touch SPACE BAR:to contimie
| op LLLCLCLCLLLLCLLLCCLCUCLCLCLLLTL
B JomBRsRi gcoineaS st e IE|)a oEmr al h Bsn;|x ereECTw3i8s 430 Eo:
A a T RRY0000 SHH 130744 RRMOTO913: EI en
+e 28.440 nTe 1h IASI
a
aa os
Go ELeS l ER aTeht0o0oDl ORIEB NT d SERIoAdi)n a Gog0oi14:53 G(e2kaSaaphsrit
Wiaant eBee
pRUOoBnSE ER Yo SRREE dl os CEaE
dE RE
TNS
ii ied SE AWC]
Er
i
002798