Document oa7qVXeMzXaDbv67ZDp6V9x3

TRADE SECRET AR226-3167 DuPont-4725 Study Title H-24516: Static, Acute, 96-Hour Screening Test with Pimephales promelas Laboratory Project ID: DuPont-4725 Author: Robert A. Hoke, Ph.D. . Study Completed on: September 26,2000 Performing Laboratory: E.I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, P.O. Box 50 Company Sanitized. Does not contain TSCA CW H-24516: Static, Acute, 96-Hour Screening Test with Pimephales promelas Substance Tested: Synonyms/Codes: Haskell Number: Composition: 24516 DuPont-4725 Known Impurities: Physical Characteristics: Stability: Sponsor: In-Life Initiated/Completed: Pale to medium tan waxy solid The test substance appeared to be stable under the conditions o f the study. E.I. du Pont de Nemours and Company Wilmington, Delaware 19898 U.S.A. September 11, 2000/September 15, 2000 .Company Sanitized. Does not contain TSCA CM -2 - H-24516: Static, Acute, 96-Hour Screening Test with Pimephales promelas DuPont-4725 SUMMARY The acute toxicity o f H-24516 to the fathead minnow, Pimephales promelas, was determined in an unaerated, 96-hour, static test. The study was conducted with 4 concentrations o f H-24516 and a dilution water control at a mean temperature o f 22.1 C (range o f 21.9-22.1 C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control and the 1,10, 100, and 1000 mg/L test concentrations were clear and colorless with undissolved H-24516 visible in all o f the test concentrations at test start. All water quality parameters were within acceptable limits during the exposure (Table 1). Exposure of fathead minnows to the water control and nominal H-24516 concentrations o f 1, 10, 100, and 1000 mg/L resulted in 0, 0, 0, 0, and 100% mortality, respectively, at the end of 96 hours (Table 2). No mortality or sublethal effects were seen in the water control test organisms. The highest nominal concentration causing no mortality at test end was 100 mg/L. The lowest mean, nominal concentration causing 100% mortality at test end was 1000 mg/L. Nominal H-24516 concentrations were used for calculation o f the LC50 value. The 96-hour LC50 value, based on nominal concentrations o f H-24516, was 316 mg/L. The results are summarized as follows: Nominal concentrations o f H-24516, mg/L dilution water control, 1, 10,100, and 1000 96-hour LC50 for H-24516, based on 316 (100 to 1000) nominal concentrations, mg/La H-24516 exhibited low concern for aquatic hazardb in an unaerated, 96-hour, static acute test using the fathead minnow, Pimephales promelas.* * Stephan, C.E. (1997). Methods for calculating an LC50. Aquatic Toxicology and Hazard Evaluation, Proceedings o f the First Annual Symposium on Aquatic Toxicology, ASTM Special Publication 634 (F.L. Mayer and J.L. Hamelink, Eds.), pp 65-84. Philadelphia. b Smrchek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation o f data. Environmental Toxicology and Risk Assessment, ASTM S T P 1179. (W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials Philadelphia. ... ' -3 - Company Sanitized. Does not contain TSCA CBS H-24516: Static, Acute, 96-Hour Screening Test with Pimephales promelas DuPont-4725 C E R T IF IC A T IO N I, the undersigned, declare that this report provides an accurate evaluation o f data obtained from this study. Work Compieteti by: MauAf rtcMjuJji______ 1 Laurie D.Bouchelle Associate Scientist W ork Completed by: *Oz*tetc/ DanielJ.C. Peterson Associate Scientist tened by Study Director: Roheit A. Hoke, PhJD. SeniorResearch Scientist Dte 36 SfS't Date Date -%>o -4 - Company Sanitized. Does not contain TSCA CBI H-24516: Static, Acute, 96-Hour Screening Test with Pimephales promelas DuPont-4725 TABLE 1 DISSOLVED OXYGEN AND pH OF CONTROL AND H-24516 TEST SOLUTIONS Nominal H-24516 Concentration (mg/L) Test Initiation (0 hours) Dissolved Oxygen (mg/L) pH Test Completion (96 hours) Dissolved Oxygen (mg/L) pH Water Control 8.7 7.8 7.0 7.3 1.0 8.7 7.7 7.0 7.4 10 8.7 7.7 7.1 7.4 100 8.7 7.9 6.9 7.5 1000 8.4 7.5 7.2a 7.6a a Measurement taken at 48 hours due to total mortality TABLE 2 MORTALITY OF Pimephales promelas, IN AN UNAERATED, STATIC, ACUTE, 96-HOUR TEST WITH H-24516 Nominal H-24516 Concentration ________ (IBS/L)________ Water Control ' 1.0 10 100 1000 Number DeadI Number at Study Start 24 Hours 48 Hours 72 Hours 96 Hours 0/10 0/10 0/10 0/10 0/10 ' 0/10 0/10 0/10 0/10 0/10 . 0/10 0/10 0/10 10/10 10/10 0/10 0/10 0/10 0/10 10/10 Qompany Sanitized. Does not contain TSCA c m -5 -