Document oMkd0RMegDQgooo402Bqgr4j3
AR 226 _ 0258
ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
IESTSUBSTANCE
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number is not noted. The test sample is
FC-803EF. Current information indicates it is a mixture of 1.30% PFOS, 10%
diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6% propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3-
Chloroallyl)hexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0%
triethanolamine, and 0.05% tolyl triazole.
-
The following summary applies a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical componentofthe test sample.
M0 ETHOD: 0000
Method: OECD Guideline 203.
Type: Acute static
GLP: No
Year completed: 1997
Species: Pimephales promelas
Supplier: In-house cultures maintained at AScl-DETD, Duluth, MN.
oxygen. Analytical monitaring: Temperature, pH, conductivity, and dissolved
Exposure period: 96-hours
Statistical methods: LCs values were calculated by the Trimmed
Spearman-Karber method. The NOEC was calculated using Fisher's Exact Test (Toxstat software, Version 3.5). Test fish age: Juveniles. Length and weight: Average length = 12.7 mm
Loading: 0.066 g/L.
Average weight = 0.0165 g (wet)
Pretreatment: None.
Test conditions:
Dilution water: Dechlorinated City of Duluth, MN, tap water (post
carbon filter) that was aerated for 24 hours prior to use.
------
Dilution water chemistry:
Hardness:
Alkalinity: pH:
48 mg/L as CaCO3
46 mg/L as CaCO; 7.80
002329
Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: The test material was added directly 0 the dilution water on a weight/volume basis and mixed by mechanical
stirring.
CStoanbcielinttyroafttihoenstdesotscihnegmriactae:l sOonlcuet.ions: Not noted. Exposure vessels: 4 L glass jars containing 2.5 L test solution. Number of replicates: 2. Number of fish per replicate: 10. WNautmebrecrheofmicsotnrcyendturraitnigontsh:e sfitvuedypl:us a blank control
pH range (0-96 hours):
7.73 - 7.83 (control exposure)
7.31-7.85 (1000 mg/L exposure) -
TCeonmdpuecrtaitvuirtey rraannggee ((00--9966 hhoouurrss)):: 21.1-21.3C 133 - 149 pmhos/cm (control exposure) 150 -- 159 pmhos/cm (1000 mg/L exposure)
Dissolved oxygen range (0-96 hours): 7.1-9.2 mg (control exposure) 6.79.4 mg/L (1000 mg/L exposure)
Remarks: Solutions were aeratedcontinuously during the exposures using single bubble aeration to minimize foaming.
R0 ESWT0 S 0000000
Nominal concentrations: Blank control, 1, 130, 216, 360, 600, and 1000 mglL Element values: 96-hour LCso = >1000 mg/L
96-hour NOEC = 1000 mg/L Element values based on nominal concentrations
RSuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTduhcetveadlounesthreepmoirxtteudraepapslydetosctrhiatbemdixintutrheeaTnedstnot the fluorochemical proportion alone.
conclusions
000000000
The test sample 96-hour LCs for fathead minnow was deferminedtobe
>1000 mglL, the highest concentration tested. Mortality observed at this concentration was 15%.
`The 96-hour No Observed Effect Concentration (NOEC) was determined to
be 1000 mglL.
002330
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
pAatAQuAlTY
0000000000
Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution.
REFERENCES
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This study was conducted by AScl Corporation, AScl-Duluth Environmental Testing Division, Duluth, MN at the request ofthe 3M Company, Lab Request number P2581, 1997.
omWER
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Last changed: 6/26/00
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STUDYTITLE ACUTE TOXICIATTYCO3F%,FCA-R6-0A3EFFF,FLTIOGHT WATER(TA)
FATHEAD MINNOW (PIMEPHALES PROMELAS)
DATA STANDARD OECD GUIDELINE 203
STUDYCOMPLETED January 28, 1997
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3.0 TEST METHODS
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5.0 REFERENCES
"WAemerricaanndPWuabsltiecwHaetaelrt.hAAPssHoAc,atWiaosnh(inAgPiHoAn),. D.19C9.5. Standard Methodsfo the Examinatiofn OGrugiadneilzianetsiofnorfoTresEtcionngoomficChCeomiocpaelrsa.tioOnEaCnDd DPeubvleilcoaptmioenntIn(fOoErmCaDt)i.on1C9e8n1t/r1,993W.asOhEiCnDg. D.C. EHadmiimlationn,gMM.edAi,anR.LCe.itaRlusCsoon,ceanntdraRt.iVo.nsTihnurTsotxoinc.ity19B7i7o.aTsaryism.meEdnSvpieraonr.maSnc.KaTsebshenrolM.et1h1o0d):for | i 714719; Correction 12 (4): 417 (1978) WWeysotmIincn,g.and University of Wyoming, TOCXoSTAT SoftwmareePackeage.TVeersiomn 3e.5. Cmheyeenne.
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