Document oMBRNJbg3pY7pQZr0jaMaBoZ7

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, or as a component of FX-3104. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted. The testsample isFX-3104, 6%. Currentinformationindicatesitisa mixtureof 0.48% PFOS, 14.09% diethyleneglycolbutylether,59% water,17.6% ethyleneglycol, 1.44% sodium oetylsulfate4,% inhibito2r.,0% polyoxyethylene monooctylphenyl ether,1.25% propane sultanefoamer, 0.03% sodium lauryl sulfatea,nd 0.1% benzotdazole. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochomical component of the testsample. METHOD: Method: Not given. Type: Acute static-renewarlange-finder GLP: No Year completed: 1985 Species: Pimephalespromelas Supplier:Commerical SupplierB,rady,NE. Analyticalmonitoring: Temperature,pH, and dissolvedoxygen. Exposure period:96-hours Statisticamlethods: LC50 valueswere calculateudsinglogistircegression methods (probiatnalysis). Test fishage: Juveniles Length and weight: Average length 41 mm Average weight 0.50g (wet) Loading: 0.67gA- Pretreatment:25 mg/L TetracyclinHeCI Test conditions: Dilutionwater: Carbon-filterweedllwater Dilutionwater chemistry:Not given. Lighting:Not given. Stock and testsolutionpreparation:The testmateriawlas added directly tothe dilutiownater on a weight/volurribeasis. Concentrations dosing rate: Solutionrenewal duringthe study. Stabilityof the testchemical solutions: Not noted. Exposure vessels: 4 L glassbeakers containing3 L testsoluton. Number of replicates:1 forexposure concentrabons,2 forblankcontrol. Number of fishper replicate:4. Number ofconcentrationsf:iveplusa blankcontrol Water chemistryduringthestudy: pH range(0-96hours): 7.6- 7.8(contreoxlposure) 7.6- 7.7(1000 nig/Lexposure) Temperature range (0-96houm): 19 - 20 OC Dissolved oxygen range (0-96houm): 7.0- 9.3mg/L (controelxposure) 4.2 - 9.3 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control,1,10, 100,500, and 1000 mg/L Element values: 96-hour LC5o = >1000 mg/L Element values based on nominal concentrations Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hour LCw forfatheadminnow was determined to be > 1000 n-g/L. Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. The studywas a range-findewrithlimited number oforganisms exposed ateach concentration.There isa lackof characterizafioonfthe testsample. Test solutionslackanalyticaclonfirmation ofthe amount offiuorochemicalproportioninthe solution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Cl 111-2,1985. OTHER Last changed: 6/26/00 AQ cj"r HEER EXPLORATORY (@ANGE-FINnER) 1 Tested LR_2Z&- -Z lot Date Bm'd 0f)@@@CI/Bc?--Ltch apg@wt-Q4Si2gram Avg.SiZ8PV C@ANo. Organismper doee -Y LoadingFactor 9/1 "ish Ddam Treatment:,4:Z,:@- L'astSolution @Ii-tm/)'-U-GlAw Muk Tank MmOmi=: 4 11ter PyrexR 61ass @ea.ker ,,ismsta@ ZL*,y Data started Date Ompleted dluticn wataw Carbon-fi-Iterewdell water (T@mp. ce I DO OS-. @5 Prm, pH W5 0 (95% Confidenoo Limits)- Tw* IniLtilalTotal 24 Hours No. COW. Amount Thtal No. D.O. mg/l Added Dead 48 Hours tal NO. PH Dead 72 Hours 96 Haws D.O. Tbtal No. pH D.O. Total No. pH D.O. ppm Dead Pt3M Dead PPM lry)e)I-Ar") T"t Temp. OC 0 Initials& Date tAf6d, qh." e.' Effeqts (otherthan M=ta3ity): TW* No. Synptms or Reactions 24-Hr. Br-50 7btai Nurba AffecW 48-Hr. 72-Hr. c1l7 IX-/K"C-I- -=w6L- 96-Hr. r*% A -,A CHEMICAL CONCENTRATION ENVIROIMNTAL LABORATORY TABLE 1. SUMMARY OF ACUTE TOXICITY OF TO FATHEAD MINNOW UNDER, ze t@) EXPOSURE CONDITIONS 24-HR. PERCENT MORTALITY 48-HR. 72-HR. CONTROL 0 0 0 0 C) 0 0 C> LC (95Z CONFIDENCE so ug/i (2) LIMITS) > IC)C)(D kooo > toc)(D (1) Data were averages of two replications for each concentration with ten (10) fish per replicate* A total of twenty fish were used per concentration. (2) W values were calculated using logistic regre-selonuethods (Probit Analysis). 50