Document oM74bv3gnD1LqmOqKV80VOp4D

AR&RE -- 0713 BIODEGRADATION (OECD 301E) TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot numbers were Antwerp, Belgium 9/82 and US 3072. The test sample is FC-203 produced in Antwerp, Belgium. Current information indicates FC-203 is a mixture of 1.34% PFOS, 35% diethylene glycol butyl ether, 37.85% water, 20% ethylene glycol, 2.66 % Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrationsof impurities. Data maynot accurately reflect degradation potential of the fluorochemical component of the test sample. METHOD Method: Modified OECD Screening Test, OECD 301E, with DOC Analysis Test type: Ready Biodegradability GLP: No Year Completed: 1983 Analytical monitoring: Dissolved organic carbon (DOC) Statistical methods: Results were determined by calculationofthe % DOC removal and graphic interpretation. Test organism source: A 50:50 mix of soil extract and secondary effluent. Fresh sandy loam agricultural soils from Ramsey and Washington Counties, MN. Secondary effluent, supernatant of activated sludge mixed liquor aeration basin, obtained from Metrow Wastewater Treatment Plant, St. Paul, MN. Test condition: - Dilution water: Deionized water Mineral Nutrient Medium: OECD medium using yeast extract, OECD medium using synthetic vitamin solution and OECD medium with reduced nitrogen content but using yeast extract. Reference and test solution preparation: Test and reference solutions were prepared for eachofthe three mineral nutrient mediums, except US 3072 only in OECD with yeast extract. Solutions were split into aliquots and put into Eflenmeyer flasks. Test materials -- - prepared to give final test concentration of 40 mg DOCI/L, reference standards at 20 mg DOC/L and 15 mg DOC/L for sodium benzoate and LAS respectively. Test vessels: One L Erlenmeyer flasks containing 500 mL test solution and capped with foam plugs. v 000458 Incubation conditions: Continuous dark conditions Temperature: 23.5 - 24C Agitation: Continuous Numberof concentrations: One plus references and blank, all in Idunpolciucaltuemfcoronedaicthionuntroinenttemsetdiniiutimattiyopne:. Not taken. Element Basis: Decrease in dissolved organic carbon compared to blanks. Test substance flasks conditions: Not recorded. RESULTS Nominal concentrations: Test materials at ~40 mg DOCIL, sodium benzoate at ~20 mg DOCIL, LAS at ~15 mg DOC/L and Blank controls. Element values: ~ FC-203 Antwerp 9/82 28-Day Degradation = 94% mean DOC removal (yeast) 28-Day Degradation = 89% mean DOC removal (vitamin) 28-Day Degradation = 35% mean DOC removal (low nitrogen) FC-203 US 3072 28-Day Degradation = 97% mean DOC removal (yeast) Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported applyto that mixture and not the fluorochemical proportion alone. CONCLUSIONS -- fFrCo-m20335%Afnotrwtehrep 9l/o8w2,nimteroagnendesegrriaedsattioo9n4b%asfoerdtohne yDeOasCtrseermioevsa,lwirtahng8e9d% mean DOC removal for the vitamin series after 28 days. FC-203 US 3072, degradation based on DOC removal was 97% after 28 days. - Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 batAQuALtY 0000 Reliability: Kiimisch ranking = 2. This study meets the criteria for quality testing. However, the sample purities were not properly characterized and ~ - tprhoepsotrutdioynliancktsheansaollyuttiiocna.l confirmationofthe amoo ffluu orocn hemit cal 000479 REFERENCES The studies were 3M Lab Request ncuomnbdeucrt9e6d11b,y t1h9e833.M Company, Environmental Laboratory, OTHER Last changed: 6/26/00 00480 ramererio TECHNICAL REPORT SUMMARY TO: TECHNICAL COMMUNICATIONS CENTER -- 201.2612 : {important Ifreportsprintedon both sides ofpaper, send two copie to TCC.) Guideolnirnoveerssa side. Modified OECD eening Test on FC=203, FC=203A, and FC-00 #003 secuiryy 0pm=Rasorts summary OoClClomdRupor-Opunsurmay|3SCR HEMI RbOI it Kosemwhaamoiscastmraree senerimanees: Twoewonos ~~ [cTunoRentTeoswteeTtihveer repeatability of the modified OECD Screening Test in measuring the biodegradability of Light Water Otter Keopmors products. LL] E[RmEPoORnT ARBAGS.TRACT Tos souac narmation s Gevio5ue1hd TechicalCommunicationsContr 1 Sr Ser 10 TthoisevasltuuadtyeagtaheinbiusoeddegrtahedabmioldiiftiyedofOEACnDtweSrcpreeLniignhgt WTaetsetr wperroeducFtCs-.203,TheFC-p2r0o3ducNtFsP s(teuqduiievdaleinntthtiosFCs-e2t03oAf),.tesatnsd tFCh-e6s0e0.prodAulcstos,tesFtCe-d203wasNFPU,.S.wasFC-a2l0s3o Liontclu30d7e2d. inOnteheof ptreesvtiionguswesreet cofonstiessttsentdonweithinpr1e98v0i.ousThOeECDresSuclrtesenionfgthis TiensdticastteuditehsatonallL-iogfht tWheatetrestperdodupcrtosd.uctsTheareresruelatdsily biodegradable. = cc: DR..LR..BRoihcokne-r2-12-3ppW--1085-10 DLW. Kent-236-pA-04 iyTy -- oo0as3:t ! jo f > MODIFIED OECD SCREENING TEST ON FC-203, FC-203A, AND FC-600 INTRODUCTION LThiigshtsWtautdeyrwa(sL/Wu)ndeprrotdaukcetns,to PdCe-6m0o0nstarnadteFC-t2h0e3,bioudsienggradtahbei)lity of two tienstteirnngatwiaosnalrleyquersetceodgnzbiyed34MGodeirfmiaendy bOEeCcDausSecreoefniinngcoTnessitstenc!). y Tihnisthe results of past biodegradation tests done on these products. MLoadbiofriaetdoryOECsDhowSecdreetnhiantg tThersetesLc/oWndpurcotdeudctsinwe1r9e80hbiyghltyhebi3odegErnavdiarbloen'm)e.psgl TFhCe-20p6r,oduacntdsFCt-e3s0t1e7d w(eFrCe-20F6C-2f0re3eAze(pprreovtieocutseldy).ideGnrteiafiteedr atshanFC-902%03ofNFPt)h,e dtihesso1l9v8e0dteosrtgianngi.c caSreebonTab(lDeOC)1 offorthaesseumm3arpyrodoufcttshewsaesrerseumlotvse.d during Table 1 Results of 1980 Testing on Light Water Products Product 7 pry % Degr2a1dation at27Day: 7s FcC--220036 w,ep} 1233 8950 9934 9913 9932 Hcy-d3r01o7q2uinone 8397 9924 9%97 9946 9935 Footnotes: 51 FPCCc--220033A.NFP is nonfreeze protected FC-203 and is equivalent to 2 EFyCd-r30o1q7uiniosnefrweaesze tpherotreecfteerdencFeC-2p0r6o.duct. pe v. These 1980 results contradict those of H. Hellmann apg,p. Muller() who TuOseCd (atotsailmiloarrgantiecstcamrebtohno)d, tetshte TrOeCsulDtesg,rahdoawteivoenr,Tesatr'e')a.n exHceelpltmiaonnn'sfrom annodrma6%llyToCobtraeimnoevdal refsourltEsC.-206Hisin ftihnedin2g1-odfay8%loTnOgCTOrCemoDveaglradfaotrioFnC-T30e1s7t c(oBnODt)radriecstulthsisweorwen B50O8D5ya/nCdOD4d0%atao.f thHeisc5h-emdiacyalbioocxhyegmeincadlemaonxdyge(nCODd)emaonfd dEeCg-r30a1d7atiaonnd FfoCr-20a6,5-rdaeyspeBcOtDivteelsyt.. TThhiesse isoppaossiitgenifriecsaunlttslewvietlhinofhis own paper clearly are an inexplicable discrepancy. HBOeDl,lm/anCnO'DsmeTaOsCurDeemgernatdsatmiaodneTebsytthreesu3lMtsEnavrieronamlesnotailncLoanbsiosrtaetnotrywiatndh by otytpihcalilnydepsehnodwenBOtD,labvoarlauteosrietso ornang3e4 AbFeFtFweepnrod6u0c%tsa.nd S90u%chofrestuheltsCOD.AFFoFrFexparmopdluec,tsamacnoulffaSccttuiroendofbysu3cMh idsatsahowonn i3nU.TSa.blemil2.itary specification 000482 " 2 OPEagCeD 2Screening July 9, 1894 - Table 2 BOD, Carbonaceous BOD, and COD Data on Mil-Spec AFFF Product Lab mBgO/D.R8) c-(Bm0gD/.k8) (mgc/okpg) BO_cDoB c-BcooDp,2 PC-C2Lo0t 5301 EY 580,000 600,000 774300,,000000 79 .81 Pace 800,000% 730,000* 810,000 serco 440,000 440,000 773370,,000000 -60 .60 Gcaaplsburlaeith 651760,,000000 667210,,000000 - 797560,,405000 `769 ``8665 Galibnroaciutlhum #1 670,000 641,000 776,4-50 .86 .82 inoculum #2 A S. Dv . erag57e855,,000000 59904,,000000 78820,,000000 a723 a725 * Calculated BOD's from different dilutions were very inconsistent. am SPaecreco GCaaplsburlaeith Galibnroaciutlhum #1 inoculum #2 PC-2Lo0t6502 290,000 290,000 338600,,000000 0.78 0.78 325400,,000000 326700,,000000 347500,,000000 00..7685 00..7802 . 421660,,500000 431210,,000000 440170,,205000 01..6032 +301..7081 ; 395,500 374,000 407,250 97 92 Sd sA.verD.age 32573,,000000 33"85,70,00000 3"339 ,000 6., "816100a 80 3 . FC-7808 Lot 501 3m PSaecreco Capsule 240,000 230,000 333200,,000000 74 7 225100,,000000 222500,,000000 325807,,000000 773 771 240,000 230,000 360,000 67 64 As.verbpa. ge 21375,,000000 23"123,,000000 3"2298,,000000 0731 "7015 000483 OECD Screening JPuagley 39, 1984 - METHODS AND MATERIALS 1. Procedure Tteessttiinngg wofascpheermfiocramlesdadinoptaecdcorMdaaync1e2, wi1t9h81t.he OECD Guidelines for 2. Chemicals T(hCe,HrNeafOe,r)encFe.W.mat=er1i4a4l.s11usaendd wleirneearlabaolrkaytlobreynzegnreadesuslofodniautmebe(nLzAoS)a.te, hU.dS.EAnSvirdoonlmuetnitoanlusPerdotweacstioan5A.g68e%ncyactaisvea sreofleurteinocnesmuaptpelriieadlbyforthe wbeiroedegUr.Sa.datFiCo-n203tesLtoitng3.072ThperovLiidgehdt Wiante1r983(L/bWy) thteestCommamteericailasl used CAnhtewmeircpalsinD9iv/i8s2ioann,d FFCC--220033 aNnFdP pFrCo-v6i00dedsabmypleEs.T.pSr.oLv.ideidn b1y980E..T.S.L. A(nntownefrrpeezperovpirdoetdectneod)lotisneuqmubeirvsalewnitthtotheFiCr-20s3aAm.ples. FC-203 NFP PthreiorOECtoD ntuetstriinegn,t tshoelutLi/oWnanadndrebfreoruegnhctetmoattehreiaflosllwoewriengdiisnsiotliavled in nDgO/C1,conacnedntLr/aWtipornosd:uctssodaipupmroxbiemnaztoealtye 4200 mmgg//ll., LATSheapLp/rWoxTiOCmatwaesly 15 ehxipgehreirentcheanhatshatshuoswend thiantprLe/vWioauts40temsgt/ilng is(20nomtg/t1o)xicb.ecauAslesoouhrigher concentrations improve the accuracy of the TOC analysis. Fsoorurctehsisalsltuodwye,d nbuytrtiheentOECsoDluStciroenesninwgereTesptr,epayreeadstuseixntgracbtothandvitamin. vsiytnatmheitnicsovuirtceamianffescotleudtiotnh.e eTxhtiesntwoasf dLo/nWedetgoraddeatteiromni.ne if the INOCULUM Tphreepairneodculiunmawcacsordaan5c0e/50wimtihxtOuErCeD opfrocseodiulreesx.tracTtheansdoils,ecownhdiacrhyweasffluent efxrtormacRtaemds,eywaasndaWmaisxhtiunrgetonofCofurensthi,essainndyMinlnoeasmotgaa.rdenThesoislesconodbtaariyned ~ esfafmlpuleenttaskeanmplferomwasanthaeerastuipoenrnabtaasnitn oaft atnheacStt.ivaPtaueld MselturdogeWmaisxteedwatleirquor TfrielattemrenptapePrlanptr,iorThteo cuosmagbei.ned inoculum was passed through Whatman 54 INSTRUMENT Tlhiemitorgiasniacpprcoaxribomnatealnyaly2zemrg oufsedcarwbasonapeDrorhlmiatenr.DC-52A. Its sensitivity 0C0484 or OPaEgCeD S4creening July 9, 1984 5 - SAMPLE HANDLING AND STORAGE mTahneufsaacmtpulreerpreofsertvhaetioorngamniecthocdarbuosnedawnaaslztyheart. prescTrhiebemdethboydthienvolves abdrdiinnggs 1thedrospampoflescontcoenlterssatetdhanHCLpHpe2.r 1T0hemlsoafmplfeisltewreerde ssatmoprleed. unTdheirs risefrthiagteratthiiosnstionravgiealsmetwhiotdh acalnumiprneusmerfvoeil L/lWinecdontcaapisn.ingOusrampelxepserifeonrce fservoemratlhismonstthusdywiwtehreoutdonaeffewcittihnign tonhee wDeOCe.k ofAllitsDOCcomapnlaeltyisoens. on samples RESULTS AND DISCUSSION Tsaobdlieum3besnuzmomaatrei,zesthetherefreerseunlctes ocfomptohuinsd,stuwdays.neaTrhliys ctoamblpeletsehloywsdetghraatded g(r9e58a)terwitthhiann 870%dayTsO.C reEmvoevraylrweiptlhiicnatetheof2a8l-ldayoftethset Lpe/rWiopdr.oducStesvenshoowfed tthheante9n0%tTesOtC frleamsokvsal.contTahienianvgeratghee vDaOrCioruesmovLa/lW pforrodualcltsfsouhroweLd/Wgrperaotdeucrt was greater than 90%. TThheeseresruelstusltsof artehisavetreasgteidn,g are and bsheocwanusegraofphitchaisl,lydoinnoFtigusrheosw t1 haendact2u. al pexaatmtpelren ogfradpehgroafda2ticounltuirnesthedegirnaddiivnigduatlhecuslatmuerpsr.oducFti,gurFeC-2303isNFaPn. It rsahpoiwds, tbhuatt otnhcaet dtehegraldaagtipornioroftothitsheproondsuecttofbedgaengratdhaattioint wwaass vvaerriyable. Tkhneownreansoornwafsor itthiesxpevcatreida.biliIttymabyetwheaevne bteheendudpuleictaotetheculftaucrtesthawtasthneot cdeuglrtaudriensgretcheeivmeadjordifcfoemrpeonntentlseveofls tohfe vLiiagbhlteWmaitecrroporrgoadnuicstmss.cTahpuasb,letohfe v cpuolptuulraetsionprioofrbatcotetrheiaonwsietth otfhesreapicdapa(beixlpiotnienetsiahla)d dteogrbaudialtdiounp. in the. RteesvtiienwgofofthtehedaottaherinLT/aWblperod3ucitnsdiaclastoesvatrhiaetdrcesounlstisderofablryeplaticadtaeys 7, 1l4e,ngtahnd of21.the Aglaaign,phatsheis pvriaorriattioontheapponesaerts otfo bbieoddueegrapdraitmiaorni.ly to the However, dhuoew tloongthebiosdmealglradnautmiboenr ooff sianmdpilveisduatlakeLn/,W ocnoentafirneiqnugenctulyltucroeulsd tonookt tell following the initial lag. 000485 Ch ! OPEaCgeD S5 creening July 9, 1984 - Table3 Biodegradation of LIGHT WATER Products and Refernce Materials Product/vitanin Source SSooddiiuumm BBeennzzooaattee//Yveeaasstt--g1,(,1) SSooddiiuumm BBeennzzooaattee//VViitt..--12 Mean FrcC--220033(/3Y)ea/svte-a2st-1 FFCC--220033//VViitt..--12 Mean UU..SS.. FFCC--220033//YYeeaasstt--12 Hean rFcC--220033a/(Y5e)a/svte-a2st-1 Mean FFCC--660000//Yveeaasstt--21 Mean LALSA/S/YYeeaasstt--21 Mean I Tm3 DOCIRemovalaAfter2X Daysom 9995..68 9834.46 95 219105.73209051 ----(4) 2287..40 os1ol.0 9925..31 22940.48 3828..66 --- 2907..27 9805..56 9853..26 92 2664..93 2950..18 =-- 9709..72 9956..50 9967..55 97 1123.162 88.95.1 = 67.76 7150.055 == 1851..10 9862.54 8976..20 92 (768.3) 8879..30 8984..82 91 252.9,8 87..88 2205..41 -3-0,8 4209..7F 35320.41 a3 Footnotes: ((33)) UUsseedd OyeEaCsDtseyxnttrheatcitcasvitvaimtianminsolsouutricoen. as vitamin source. (3) (5) 1Fr7o-mdayAntswaemrpples19w82e.re only taken for LAS. = (8) sFarmompleAntcwoenrtpami1n9a8t0e.d, Orniotginuaseldl.y labelled FC-203 NFP. 000486 READY BIODEGRADABILITY: Modified Screening Test - OECD 301E -- Adopted 12 May 81 Environmental Lab Request No. 9611 2S | T | = he 0. Le 3g = T T [| THIS SERIES HAS RUN USING i l {| C"OSNTTAANIDANRIDN"G YNEUATSRTIENETXTRSAOCLTU.TION | nbn 4 as o= T eff rffr: e ; L. fm Jon7sme z= / of ~ Lso - L | -- AT fersa a / gpd wf y | Re . 1 ir J& B ! 0 = roe Lo i bie [SDS2I ORE,FTTRREE. 0000TNT.. of | Emien 2 oX i ] 7 oHe &] pe 2paree "#2 READY BIODEGRADABILITY: Modified Screening Test OECD 301E --Adopted 12 May 81 Environ, mental Lab: Request No; . 9611 #} STTHAINSDASREDRIENSUTHRAISENRTUNSOULSUITNIGON || | WITH VITAMIN SOLUTION. i J 3 0 ammnns ffm g i geggB 40 -- Frrerrsmersefraresssesss | / } a i | EE ye LS ; fms 7ya | :- < . g2 s z] :i J] : qa AEN WSSU ES yan i 3 0 // ii A i g, i i 2 7 ~ on)ts pasts i Legend i |a soo pozonTE REFERENCE ;i x Fo-meameuves, & = , READY BIODEGRADABILITY: Modified Screening Test Co OECD 301E -- Adopted 12 May 81 Envp ironmentalr LabRequest No. 9611 903 5" EE THIS SERIES WAS RUN USING | 'STANDARD' NUTRIENT SOLUTION F-- CONTAINING YEASTEXTRACT. i i i ------ -- [.. , 8En |i // | gw re i| -- 2ze Lo YA | ! a E odd . E& = |i il :i | ii. | %g mem / eb| er3 fn] wo E2 e gc } i + :i :i 7, os Legend 7 la poems, mere `a0we rusvo. :i [xmam,moe o=weeus. al 2 ot wh READY BIODEGRADABILITY: Modified Screening Test : OECD 301E -- Adopted 12 May 81 Environmental Lab Request No. 9611 1003- THIS SERIES WAS RUN USING T | C"OSNTTAANIDNAIRNDG NYEUATSRTIENEXTTRSAOCLTU.TION ii THE LAS REFERENCE WAS DILUTED i WITH FRESH NUTRIENT SOLUTION T| T); 2 3 ON TEST AY 28 Ha S-ita gre. hi cl / | sol ||| i. El | tt | Lo g i i | I fd i I /Logond i Iara A us rere 2 | ! | Bu us mm rmes cs T a 33 DAYS . f OPaEgCeD S9creening July 9, 1984 in . The modified substances. LOE/CWDprSocdruecetnsi,ngonTestthe isothienrtenhdaendd, foarreusaequeoonussinsgolleutcihoenmsical ctohanntai7n0i%nglosasmiofxtuDrOeC wofithcihnemi2c8aldsa.ys, Puarree crheegmaircdaelds,bywhtihcehOEhCavDe greater grueiadcehledinewsithaisnbe10ingdayrseadofilythebioDdOCegrraedmaobvlael. EirAsltso,excteheidsinrgesu1l0t8. hasOnltyo be one of second OtEheCDrerpelqiuciarteemsentofofeac7h0%ofdegtrheadaftoiuornLw/iWthpirnodu1c0tsdaytsestofed fmiertst this rreeaaclhiisntgica1l0%lydeagprpaldiactaibolne. toThLi/sW orrequoitrheemrenfto,rmuhloawetveedr,proisducntost d(imfifxetruirensg).susc~Tehpeticbhielmiitciaelss wtiothbiinodmegirxatduarteisonw.ill Rfeardeiqluyentdleygrahdaaveble cwhheimliecatlhecoonmspeotnenotfsdoefgrasducahtiopnrodoufctosthmeraychsetmairctaltso diengrtahdeese ipmrmoedduicatteslym,ay rbeeadseolnaaybelde. forThumsi,xtduriessregofardcihnegmictahliss, stehceondrecsruilttesrisah,owwhtihcaht tishenmoitxture obfiodcehgermaidcaabllec.omponents in each of these products is readily CaulstluirgehstlytesltoewderwiltehveltheofsyDnOtCherteimcovevditatmhiann scoulltuutrieosn ushsoiwnegd,yeaonstaveexrtargaec,t ayseasttheevxittraamcitn sisnocurecet.he FsuytnutrheetiOcECDvitsacmrienenisnogluttieosnts isshmoourlde thus use difficult to prepare and does not give improved degradation. dLeASt,ergwehnitcsh, idsida nroetaddileygrabdieodefgurlaldyabulnederandthecsoemmotnelsyt ucsoendditsiuornfsa.ctanTthisin oocfcuDrOrCedcomipnarsepditetoofthethe40 fmagc/tl thOaft DOiCtsusceodncefnotrratthieonLiwgahst Woantlyer15 mg/l ptroodbuecttsh.at TLhAegcirnehasiobnitefdormitchreobsilaolw dreagteradofatiLoAnS batiodtehegratdeasttio7n appears ctoenstcenpterraitodi,onthe . re"mTaoincihnegck LAtShisconhtyapiotnhiensgist,estatcutlhetureensd owferethespl2i8t-daaynd hmaeldfia.wereAtditlhuitsedpoitnot,twitchee trhaetierofvolduemgeradwaitthionfreisnhthienodciulluatteedd ncuutlrtiuernets tiinmcereaosfeddilsuutbsitoanntwiaasllrye.moveSdevewnittyhinper1c5endtayso.f thReesuDlOtCsroefmaitnhiinsgwoartktohne LsAiSmilaarerlyshorwenpogrtreadphiacallolwyleivnelFiogfurebio4.degrAandoatthieornroefseaLrAcSheirn, thheasmodifi-ed OsEulCfDonsactree)enihnagd taeshta.lf lD.ifeLiouffo39unddaytsh.af.Marlon A (dodecylbenzene CONCLUSIONS TTehsetspreosnenLtighsttuWdaiteesrreppreoadtuectds tdhoenefibnydintghse o3fM pErnevviiroounsmeOntEaClD LSacbroereantionrgy. Bboitohdegsreatdsabolfe.t"estsInsahlolwedcastehsa,t Lt/heW apvreordaugcetsDaOrCe r"emroevaadlilyfor L/W pro-ducts dwaesgragdraebatleer thtahnan th9e0%vearfytercom28modnalyys.usedL/Wandpro"druecatdsilywerbeiomduecghradmaobrlee" surfactant, LAS. 000491 [ I. b- = oPaogne g10ureentng July 9, 1984 12 - REFERENCES 1) oFrorganTiezsatitnigonofforCheEmciocnaolmsi,c CSoeocpteiroanti3o01n aE,ndReDaedvyeloBpimoednetg,radGaubiidleiltiynses Modified OECD Screening Test, May 12, 1981. (2) &6./80A,. R3eMinTeerc,hniBciaoldegRreapdoarttioNno.of40,"LDIeGpHtT. WA0T5E35R," 6/30/80. PPrroodjuecctts 99i7n00O1E2C6D00T,est (3) pErx.tinu.guiHsehlilnmgannFoaamndCoD.mpMouulnldesr,(U"nwEenlvtigreofnamherndtuanlg Idmupraccth of Fire WIFeosunterGsleorstmcahni-gethauunmtitoetefil.H)ydroTS lheogy t (Buwnu daessaCnd asrtraily etd fouurt by the gewasserkunde), April 18, 1979. 4) bTA.ebsbtamuutlOelrsegtrani~acndeMinaT.teVrTeiiratfltasihzreefrno,r zCu"orThmepPlrueTftOueCngDBeigvoordnaedgaOrtraigdaoanntbiioTsnecsht(eDn-erASTuObPCsrto=acnezsesnto `sBuf uVonlTsdtanedifsgoenraGbeTnwoalsossgeTrstkeuhnaoinsl,AbKbtoabul-eFnz, De7v.elFo.pedWaseart-utnhde ot Abwasser-Forschung (1979). 5) bohrman DC-52A Operating Manual, 4th Ed., 1978. (6) M,arcpbe.lSwDieskhkeerr,, SIunrc.f,act1a9n70t: Biodegradation, pages 16-17 and 147, (7) bB.lodLeiut,risotruadtyioonn B5,iodEedgirtaeddatbiyonT. ofA.,AOnxillienyeanadndS.MarBlaornryA,, 1983. 000492 fom 12h APO Sample Description Lab eauNo.e2s42t/. Environmental Lab -- Work Sheet Z . 7 1, 5 ti owe 7/24/43 [ANA YN - TT at na spe = Fill cavboy ~[0 Leite Df Fao ----) Tok CaCl, Sebiiod [lad Fee ste 19) Tae tnt sbolies --pact ZC2 Oy] Shodancd k= vn gt tebidiel y OL tn poludicn . ~\~uhUtON Yeo Wk witihti Sl Keobef 4.0ualVhs So Luo nl paulscbutats Saas le bo 6.0L wh atric with oboet on . arbi 000493 famroizon ammo : Un neesto,Z64/ SampleDescription Priporitioi Environmental Lab -- Work Sheet 4 Tost Sltons one: 2726-13 anys: LQ SRe n'D Dilts, (, -- < Hest and pufrnemes pmitineil wrth lol ff ibid pebeiiod x Gras 0. 19y 0.154 0.2 0.0343 0.815 _ o.4% Mera Fe103 Let 3072 Fe-203 ((Awrwear fez) Fc-203 (ANTWERP 1980 NER) forearm berg cnte Las (07. Active) FCloo(Auweer 9fn) 0000 lteons Svlenme., Cp lik abr 500 mb a ord put inh 10C ks. cht fosh--toasr Ltn hod 9 TAT 72 cnt!geegmncd ising wmattuatlin from HeMpls [still a 4 J . : " 000494 [FE CYA Sample Description D. tutors Environmental Lab -- Work Sheet one: 524-83 Ansys bud.QeSo ZO LAS Teaz Spbuformtwn Jeo?L. 29 Zien LAS Aafornimn copra Fot polilionid avel. Fre as C0: A Leeote Mar tes Lon He "STANDARD" denice i} . ol 7 77 frreectens tton Z Concoidhalari 100 =m L af S-gk~ S-Bk-2, S-tAS-1 amd S-1AS-2 ire Tosnotoned To pasate lade. bo --deddiCtoitct.iad [0000 of Sts7foredd pond sutra" Neon rp lated pzd Sod i fib1er altos fot ml [200ml ). Ls: The yr Lxleal Pantenig lise as Raa S . = aas a wh : 7 = Seedgree | 50:60 py ff daminclryafflent amok el wre a $0:50 tone oY ERY pander) Corrs poi 0 pcem [thadoi's fons Cou geil. anid ends . 000495 wotohen soon --_-- ---- EEAPC De arSorvino - Results: on Adtid Lb neues TCL - LAS Fach onbetorns) ikl fon pogritiod one Z24-83 Cy Anat fa Qe 2RZD eso Lasap1) lm teZeterBy2292 lefey -- Zorn: LAS pufirimen compre Set pobliTionis) arel. Fico adilAL aidTt a 0eee0 clir0 e. 0000000000 ZL conceidhadani v 100 =m L af S-Bk-1 S-Bk-2, S-tAS-) al S-ias-a Loafing ate flock. 4 _Bldlirnntal [00nlofSehlfoiodt prspiidnacpid Neoiinsbatd snd O-Cod initio) po Libor ff orlitos dalled Ao . rsas Ha foci, pComandonlei2 of toHho dlotel CZ = Seedperce| 50:60 iy Uf denenclens affluentand el wre a $0:SD pivtrone of ERT andor) Cotrrs fri pre ledatiioar Lous axl. an e 000496 Gr Rl... i \ Sump Dwsrition Rei o1f 65 Tint Sutin -_-- woman, 964 oun S2Y-83 vay: ba.Qc20 Gotuy 25 ge LAS pr eo em arm Patra L2. ( Geceted LO. fir) Frans hs "STANDARD" dinicy 2 foe AlSoLASd Dra liri v 100 -mt far S-Bk-2 | S-LAS- tos Liasfnnd Te gpsiate flack)Ge Atsnsradelid suid O-Cond lniieloon) pos Liberff orbits . Lz C , T wa I a E Seed dovisn 50D min ff hirer fflntood il Twirteetoiand SPOlDandpoyp(bpionteater5sEy fRoaYls 2 pds dist!ifGodgoHshoourintd, Cotrrs il Poe ltd ede tons Loita 000497 + rom 108308 ATWO Sump Duicrigtion D EN 2 Alek ie dnt Lav oqueFtGtio Environmental Lab -- Work Sheet oni S2Y-83 nay:a).Qe 52 RO Aas Te Slit. Teo? 2 29 lan LAS tefrenan Compre Fest goliBiona)ancl Tove a 00d Qeeeticl Llanes) fon Hy STAN0ARerDic" " 7 , 7 2v 7 L- Dra lori 7 7 oo A se S-Bie-2 | S-tAS|- . 47 oo. . Seedporesi 50050 pip 4] dencclers affliusntand aril wae a $0:SD tore o7F eel 252. Gril unde; 000498 A Lab RegueNsot, 26.4] [---- Environmental Lab -- Work Sheet oni fo=C83 sou: L2G.Ski0 0000M0 ey00000000 ting OECD" might 30IE , Riad Biorclegnadadilii : Hindle bco" Sensis, Tout " Lorwtnit stiblennd sem nutes Bele Tervo vatindiond ane mmpatiacs oF nig eis Zo a. Aa Nemes) and! ator [or ebat 0b genie] abil pris setitess, = nies fiatatin once psd sil LiferL yudhaed Qotulerna or Selling) i 1) MuZag Urluliiciv tortoerir yeaat ciact de as a. Oubofite ans > ye " - ola lire fone, yaad sxliect. 2 & daditits Lo tari, alent art a 9ST dower on Bren ype, - . S -- The vSaraies) ali- ili- na)avsfray piind. using Habel. vahieZion 2. Soc alfaclid sled LZUt PupeneTion oF Nithund Sdiluns, " snl = floes 000499 form vosios ius : Sample Desription " CT ie Lab RequesNot. _261/ Environmental Lab -- Work Sheet one: SES Ansys: LdShei Doll Ztrng Verncio. frmaelenabl, olenive irxdirmidlot deznpy Levy pencils) (Aa Cet Rus yanea Lecr Wen ly Ceci am Zh, Lorgthl of AMrietigractaicts_suirie7 Duce ag obass tha! FLSupa or log pdaak Nag ohict , Aho egiodtaZarsd Nails fA iplitatsc datiFuneBri deinalonn " tant. of loner 7 5 5 Soria iri gunz. before 7 4 wmJmpemttehIAS ilin | de dam7 p ling Ray,J `oon tn bet pr;moctirnaddpmhiatice in thd - obsse fuod afin fhe serpin dag. - bocavor of um fhegiipar Borg lov erent) [roens, ~ Tela OTs? ff GAL 7 tt pone-- nt V Gl PAG din.e no & Clzl . ,A 2 aA cation Io Airy tn) foc a clic, Acs qaNRTEN PE L r 5 NinelTs ee [ 7 es, "pe si pte Adpminiibso -- - 060500