Document oDzY1ndyadRaqR6bZzEV6qVe8
BIODEGRADATION (ABS/LAS Shake Culture Test) TEST SUBSTANCE Identity: PaesrfPlFuoOrAooacmtamnooinciaucmids,alat,mmAomnmiounmiusamltp;emrfalyuoarlosooctbaenoraetfee,rrPedFOt,o FC-116, FC-126, FC-169, or FC-143. (Octanoic acid,
pentadecafiuoro-, ammonium salt, CAS # 3825-26-1)
Remarks:numTbheer tweastss8u3b.sTtahnecteesits saawmhpiltee pisowrdeeferr.reTd htoe b3yMtphreotdeuscttiinogn lot lsuafbfoircaitenotrlyyacshaFrCa-c1te4r3i.zeTd,healptuhroituygohfctuhreresnatmipnlfeorwmaatsionnotindicates itis a mixture.of 96.5 - 100% test substance and 0 -- 3.5% C6, C7, and C8 perfluoro analogue compounds.
METHOD
AMsestohcoiadtsi:on'SshaprkeesuCumlpttuirveestteusdtyfomrotdheeldeedtearfmteirnatthieoSnoofapABaSnd/LDAeStergent biodegradabilty. Type: Aerobic GLP: No YCoenatraccotmtpilmeete(dun:its1)9:782.5 months Inoculum: Activated sludge collected at the 3M Chemolite Facilty, CWoatsttaegweatGerrovTer,eaMtNm,en3tMPlDaencta,tSutr PFaacuill,itMy,NDecatur AL, and Metro RESULTS No biodegradation was observed in the 2.5 month shake culture biodegradation study. DATA QUALITY
Reliability: Klimisch ranking = 2. This study meets all criteria for quality
tseasmtipnlge, wbuatstnhoetansuaflfyitciiceanltlmyecthhaoradcotleorgiyzeids.questionable. The purity of the
03633
REFERENCES
EFaAt.e RoefiFnleuro,rJouclhyem1i9,ca1l9s78in,t3hMe ECnovmipraonnmye,ntE,nvPirroojencmtenntuamlbLearbo8r8a7t0o6ry1.2613, OTHER `StS.ubPmaiutlt,erM:inne3sMotCao,m5p5a1n3y3, Environmental Laboratory, P.O. Box 33331, Last changed: 5/25/00
03634
Form 6147110
TECHNICAL REPORT SUMMARY
To: TECHNICAL COMMAEATIONS CENTER -- 01300
inoran= sort an sfpi, tvcpr 0TEC)
"7/19/78
EFantveiorofnmeFnituaolrocLahbeosrtateoarlys in(EtEhe& PECnvironment
oo0r5o3s5ze10
Biodegradation Stuttes of Pluorocarbons - 111
:
5. 1. Bacon
=. one
rei
=
or
. P. 6-14, 21, 25-27, 20,35, 39-43, 46; NeoFmmTrduan
wE eoomrnvT ,y O4e57m27,Corp. n32o-3k5v;id49400, p. HEE 11-12.
ra17 g -
oe Ttoo eavlaclruoantieathdeecosmupsocseipttiiobni:lities of FC-95 and FC-143
(EFBmnlivouuidorer.gEarCcahhhdaieasymet.oiisao.ni)
Dograastion TE A TL em To om rae i RS
A biodegradation study is described which allows the evaluation of the susceptibility of FC-95 and FC-143
foaetonic microbial degracktion. The cultering peor cedures used in this study are modeled after the Soap
and Detergent Association's presumptive (shake culture) test for the determination of ABS/LAS biodegradability. Microbial inocula were obtained from activated sludge
collscted at Cnomolite; Decstur and Metre waste treatpgnt plants. Analytical procedures included GLC,
TLC, ""C-scintillation counting and analysis for released
the Sultanality of the biosesradacion tent conditions: fluoride. Degradation of reference compounds demonstrated
nan
wie Spl)
03635
pe
sumuaRy
FrleusoirsotcahnetmitcoalbsioFdCe-g9r5adaatnidonFC-in143a tion study, The mixed microbial
w2e3r-emosnhtohwnshatkoebeculctoumrpelebtieolydegradatest cultures used in this sgudy were
dmceeunrltitvuesrdyessttewrmeosrme a(Ccmhtaeiimvnoatltaieitdnee,sdlDuiedncgaedtiuilrnu,otceu&lyatehaeosbttTwaeiixnnterdCaicttfi-rcbosamsatMlherterseealwptlasasntmtee)d.tiraeTahet.
siiuc-apdlposld.emceenTnetese-tddewrciiutvlhetdurtehlseinaehyaldrsrooaglcekonynltaanisnauelldofgonFaCotf-e95th(oeLrASr)FeCsw-p1oe4rc3et,ivuesPehdfelnauosolrocahnedm-
gtrrreaafdneasrtfeienocrneredccoomu1p5lodutnoidcmsce.usroTvuhenerdierrthedtehgeZrja-tdemasottnitohcnonpdediretimoiodon,nsst.arnadteALdtLemthpcaeutrlatbtuiuroredesew-wearse
controlled at 25 C. during the latter half of the experiment.
aItnnldutohgraeoschfelininiaqlcuaidlgsrcowwhotrrhoemapetarosigsoraday,pehdydef(ogGrrLaCbd)ya.titohniCnhe-pmlriaocydaeulrcstscsherofpoamra"atCto-egldraabbpeyhlyeTdL(CTLC)
wceurleturveiseuxatlriazcetdsbyseTpLaCr-aatuetdorbaydiGoLgCrawpehr.e No degradation products were detected.
dMeettehcytleadtebdy aelnedctnroonnotchapytluartee.d Scintillation counting
schheomwiecdaltshatremaallinerdadiinoactthievictuyltuarsesomceidaituemd.with the labeled fluoro
mcIononnicatelolnrterbdauttisoitnmhpeliynfibntyahlememgeardsoiwuatr.hinpgeNrotihoeidn,cirneifatlsiueaolroicnaanrdfbloufnoirnbiaidleodfelcguoronarcdieadntetiroantiownas nwoats orbessuelrtvedininthdeicarteilnegasethaotf iffluboriioddee.gradCaotnitornoldicdultocuoruers, suiptpldeimdented wtihtehexfpleuroirmiednetaslhowceodnditthiatonsfluusoerdi.de is not lost from the media under
Wcthhoieulledrebsteuhlistfsosuotnfuddytthhaictsaiwsnotouutdlydrulaseulglgooewusttthtehtehbatipoodsteshgiesrbeaidlaictthiyeomnitchaoalftstchoaenrsedeitliciooknmesployuntdos, pmeircsriosbtialincattheaboelnivsimr.onment for extended periods unaltered by
03636
eee tt tre eter eee -
InTRODUCTION
The fluorochentcals selected for this study, FC-143 and FC-5,
hThaovepeprefrlfulcuroirniantaetdedpocratriboonn ocfhaifnlssoarncdarabroenschneamviecalnley estmasblef.ound
btoiodbeegrsaudsacteipotniblsetudtioesbiowleorgeiccaolndudcetgordadaontiotnhes(e1).som`Tohuenrtesforpee,imarily
for the sake
Dboedistaiicdatwiiotnh.
of completeness. Without such certainty that these compounds
testing, it could not would resist microbial
tSfiirononcmseabrfieonorsdebgicrooadndesagitrdiaeodrnaetdwiao1snikweulonyrleikteosleylc,eocnttteahdie.nbeasctIenloifcmeuaalssseiwdb:lemeeictreoosnrtreaecinoeenndeim-s.
Long acclimation periods-were used in an attempt to select develop populations of microbes capable of degrading these
and compounds,
s2uonoldrocohtycdarororggoeannnsioannsalotghsatofWEthNe Gfrlautoertoiceaursbioynsewoemreetaabdodteidzetso rthrby to
03637
an
METHODS AND MATERIALS
Chemicals
cF(aCtr-h9be5o,nh-y1dF4rC-o1gl4ea3nb,elaentdahleFogCh-y9do5rfogwFeeCnr-1e4a3on)ba,tlaogicnaoerfbdonfF-Cr1-o49m5,CloamabmmeemlreocdniiauFlCm-1oC4hc3et,maincoaaanltdse Doitvhiesriwoins.e (TAhretsheurcMheenmdiecla-lsRepwoerrte uinsePdroagsrersesc)e.ived unless designated LScatobamonprdoaautrnoddrylfoirinneabCriiondaceligknrynaladatatite,iosnOuhlifsoot.nuadtieeEsxcpwerpaetspawrohebedtraeifnorneodtuesfder,oamsaltlhaeorUtehSfe/erErPeAnce compounds were reagent grade.
Culture Media
-
TinheTAcBoLnEtro1l. medium used in these studies had the composition shown
TABLE 1
CONTROL MEDIUM COMPOSITION
1) Basal salts solutions:
1.0 8/1 - NE,C1 2.0 g/1 = K,HPO, 0.25 g/1 - MgS0,-7H,0 0.002 g/1 -FeSO," 7,0 2) Well water - 25 mi/1 3) Yeast extract - 0.3 g/1 4) oHrydFrCo-g1en43an-a2l0ogsmg/o1f either FC-95
Mberdoiuaghtwerteo pvroelpuameredjusftropmrisotrocktoseoalcuhtiocnusltuwrheichtrwaensrfeerc.ombiAnefdresahnd
sionlumteidoina adjusted
opfrepFaerSaOt,i.o7nH?,a0twaesachpretpraanrsefdera.nd Tdhrey pyHeaosft aelxltramcetdiwaaswasused to 7.5 with 1.0 N HCI and if overshot adjusted back with
pt1e.r0raicoeNdeNalpOerHmi.eonrtstT.ohethAweenlalliynswieatstieatorfiownatsheofawdedtlehlidswtaostteuirdnysmuasrdheeowanedduardiientsqguactthaeelcsi1uu2pm-pmloynthof
hiatradtenesrsesutoltirnanggefrofmromthe92 adtdoit1i4o4nmgo/flweelxlprewsasteedr filtration through a #54 Whatman filter.
wCaasCOF.emoAvneyd
pbryecip-
03638
re.
Tbhieodepgurraidfaiteidonhydtroosgtenmedainaaloagnsd cofontFrCo-l9s5. anTdheFsCe-14c3ompwouenrdesuasecdein dciieanngracrbtlaoiudnode-nehdyrtdheirenaocgtfaeinlnounoasbrtootnceadamrrepbtcolfnertsaoe.vqausgeeeEnlntelcz(yty1)m.eaisdmeinAcctdairdpcioaatbbliiloaenltaoolfPoepnccuzoaylmtpmaaoetinsiyeoenniitnassgloiloSkefeeelfdyio.utchtieenoer Specific media are listed in TABLE 2.
TABLE 2 GROWTH MEDIA FORMULATIONS
Media
_
c-05
FC-143 Test
Phenol Controls
LAS Controls
Fluoride Controls
components FC-95 Control Medium + 50 mg/l FC-95 FC-143 Control Medium + 50 mg/l FC-143 F3C0-9m5g/1orPheCn-o1l43 Control Medium + FC-95 or FC-143 Control Medium + b3e0nzmegn/elsuSltfaonndaatred L(iLnASe)ar AlkylF3C3.-295mog/r1FNCa-F143(1C5o.0ntrmogl/1MFed)ium +
Yic_rc-gs Test
FC-95 Control Medium + 50 mg/l
Yc pegs
140-PC-143 Test .
FC-143 Control Medium + 50 mg/l
Ye_pe-143
FC-95 + LAS
FC-95 Control Medium + 30 mg/l LAS + 50 mg/l FC-95
FC-143 + LAS
Culturing Procedures
+F7C5-0143mgC/lontFrCo-l143Medium + 30 mg/l LAS
Tmheediuimniwtiiatlh sludge used
w1garsmolwtahofmpiaexcrttiuiordveawtofaesdtsswlotuadrsgtleeuddgsebusypercinonalotlcaeuncltta.etdinTognhe49thaemcltdiaovyfateoefadch
CCiohnneotmcrouollliatteCioonmW.naisstesTihoTenr'essaltumMdeegntetrowPalpsalnaotnbttianiinnCeoSdtatifnartgoemPaGturhloe,veM,MeitHnrinonepnsoeoltsiaet.tann.anWdastthee
03639
"-
Following inoculation, the cultures in polypropylene Erlenmeyer flasks were shaken at 200 rpm on rotary shakers at room temperature 4).
Ai(tdieetn.ht,eicae0l.n5a fmorlfesheoafcmheexdiigssrtoiwutnshginpgceurlaitou1dr%,e ietnaoocch4u0l-uc5mulmtiiurroeomfwtahlseenbttrroascnoesntsfiennrgorewdcumletydouirone). TAih'ne1o0cugmlrlaotwitsohanmppleoerriowcdausltbuetrtaswkeenetnrafnrtsorfmaenersafceahrnsdcuvaltatruitreheed eaasntdi1os0fmnieonatcuehtdesginroawTftAthBeLrEper3i.od. oSnfaomtpetlhdeesincwoeTnrAterBiLfEcuegn5a.ttrei.fugDeedvifaotrio1n0smifnr.omatthi1s7,0c0u0ltuxrinpgrpiorwocevdeuraenaiapvseis
pCTrhueelptaufrrieendsalwwiegtrrheowtCgharrgpbmeorn-ii1no4d-flldaaibsfekfsleerdoendFCfa-r5ro5omtaparnrydecseFhCda-i1kn9eg3r.peinrOionadesG.hruonwtdMhyeeddicahmaiwmebreer codnatyrsollaendd aatt 3750dayCs.. + 1. Twenty ml samples were taken at 10 min.
Chemical Analysts
Fluoride ion concentrations were measured using a fluoride ion
eatalhnceedoscuetraratosfedtlleauynodr(aipOrdrrdeeiponacsrtuearidnvodenafradldrnusaaowrlnbiydrzeaefcrrkosemtfatlneutddohaerritddhrseee.suecllotTenshcceternooftcdroeamntecimaeoosnnddtserrleamptroi9eno6stn-ees0n9t)oo,ffin
tshaempleixnpgeripneerniotda,l this transfer, &
es1xa.c0mepplptepsm.fofrlFuloturrioadrneisdfeesrtacnui.dravredsForwwaesrteneusseeacd.naultpyyseacssaliefbaorgrhattoewing
tFhleuoriindsetrcuuernvte wrietmhaintehde acosnssutmapntti.on hat tho Slope of the previews
aEPhxleanmkoilnyaTtainboanleynsoifzseWwanatsesrsdoa(unnLedlASfa)acocswotnarwssdaittnaegnsra,tloyz14SSettdhandfdoarridbyMtethtehio1md9eso7t5hf.yonlrent,ehTienebair e,
chloroform extraction method described in the 14th edition of
sSby0atmapnatlhdeemasorddsiwefapMisaecrtaahdttoiidolosrunyte(od3ff)u,tnontehelix1sc0we0pemtremtlehion2id5n.trmaiasnIesnopffaetrrhSasitstao8nr-mdy1ao4rd,diffuMinLeneAeStalh.omwdaesschaAemlndesa,tolhyyiszlhdeeeddneed
bdflrouarewn3S0oolfsufetciotnohdnrso,uagnhdalgl1lo0a0wsesdml twooooflsecthitlnlotero,ofaosrw2mi..r5lecndT',hidsiaanmmdeitxtethreu,recShpwleaocsros2f0ho.arkmeeunrvette.
PceurrcveentpretpraarnesdmitwittahnceSuwrafsactraenatd Treated in the same manner.
satam6p5l3esnmofaknndowcnomGpoanrceedntseoataioantandurd
03640
a 7
TABLE 3
IN STHUEMMASRHYAKEOFFLCAUSLKTUBRIIONDGEGPRRAODCAETDIUORNESSTUUSDEYD OF FC-95 AND FC-143
Transfer #
Culture Growth Period (days)
Notes
3
MUesterdo aacntdivCahteemdolsiltued.ge
inoculum .
from
1
3
cFuCl-t1u4r3e-shydarnodgecnontarnoallso.g added to 143
2
4
3
3
A1tmlthoef tDiemceatoufrcsullutdugree
transfer, supernatant
added to cultures.
4
3
LcoAmSporuenpdl.ac~edLASphemneodliaaswasa rienfoecruelnacteed
waintdhChaemmioxltiutreeanodf
control Decatur
culture sludge
supernatant.
5
3
6
3
Tdhiescounsteinoufedf.luoride control was
7
6
Spohsaskiebrlywasforin5addvaeyrst,entdluyrintgurntehdisoff,
growth period.
8
3
w3asml
aodfded
Metro to all
cuslltuudrgees.supernatant
6
10
4
:
1
4
cInulttuhriess awnedresubgrsoewqnuenint garorwetchippreorciaotdisn,g
msihna.kera-ndwat25eg Cb.ath at 100 strokes per
12
6
13
6
14
8
-
+6 (2)
15
7
78 days = Total Enrichment Period
03641
i.
Carbon 14 Counting Techniques Sctntillaion counting vas pepigraed on 1 ml samples of culture Saneneepalsa oorrreectetiohnsi.c sThaeo'piroasdioofsciivHOiLsEyE.o0f) htohmegsTcaolmmeass
added directly to Aquasol.
or Leon or at Set sao eetath Solid samples were collected directly oonitooomtilbluirpeosreabHtAra0i.e45 umhoe CCCCfoihRmolobtAumensrGsttiheeowmtneakirenrevoeaa.tsrthhaeestnrdapwbCcpeaooetsudthn.netwadeittdchiwhooiinntC'ohsBndcobeaieoreuipiteiopnionianhigsioUzeanVee'ti.dsiEozwsnPaaTthcFeskurasaredannlddicBcoopeptltnlsaecatceciiedeiiddia,1ts2iiinnotttenonpMpeuarpier counter. orrSeaemnpileens. were recounted with an internal standard for
mTTTToheehtiorisinenab--noLl:ClaaoyytoteeirerorsnCtcahobhorfodroTuomtgaAagntdtF3ooEgmgsrormnaam0tppe5hhhd,.yyina(twnTeFaaLlasyyCg)eHsSrOrafLoeorspCreiInneR:esedS.wMtroeereedsnxetrmmeencantstctirueearrddiemoTseaorcnsttaiilrPveeioitrctessa
Pr I
tral ae
nSTcAiFhecxenectttiarh"rteioderfr",uiacghweyaJdSitaoemamreati,.dee1sst`7aaa,itvm0deep0rls0beom.slaxinesdcgpeehutisho`phsoehreaneensdvseoasusd.rpveoarpTaattothrhseeeedtweSseaoeiberpeaerhrhBaoetBpmohiamoomnenneys'eao,4eftf7asudstl,n,hdepseoosrrtettt1eiihpolynllle
TwrTshopreesoeltatptlirEeanettgrewoosowrreanwernoecresoend1rseoESss.euevsasetpBpnloeesotrntpetdkoeenddasmwdiiiiinrrilrehmoieertts1t.hp0ya.e8nnooeftltoohI.hr).i)afesiobfriTeeh-eibpsnahirsetesushvoeueotssssepnednpaedcidecHlidilcipcLsuesaatmminepspollaileasse
VfiSouEirizeed!by exporting Hoduk no-serecn acray tI bn There, 0d
TLC vas repeated on the remaining portion of the solvent samples.
THaFeheaedviasdloeyl,verne"tsSuowsvapesoepnedadeldloaswinebdemfteootrhee.avanpaeonrdatTvheiooseteco.lSdlraiystnseessBsiwteiennss aSnihro,ottiaEnngddttihmeceree
03642
"o-
Gas-Liquid Chromatography (GLC)
EYtahyyelr cachertoantgejogerxatprhayctsmotwhegrdes.preCpoanrterdolassodleustcriiobnesdweirnetmheadethbiyn-
t4hiessoot1hvyilngaceTtIaCt-eFC-e9x5traancdt sLaImCp-lFCe-s14a3ndintehtehyelthyalcetaacteet.atePocrotnitornosl of
solutionswere nonmethylated
aeltshoylmeatcheytlaatteede.xtraAcltisquoatnsd
ocfonttrheolsmewtehryelatiendjeacntded
ocnatpoturtehede5t7e1c3toHre.wletMtetPhayclkaatredd sgaasmplecshrwoemraetogirnajpehctweidtwhitehlienctr3onbrs.
o1f/8"th0e.iDr. mestthayilnalteisosn.SteeTlhepacchkreodmawtiotghrap2h0i%cDCcol2u0m0n (w1a2s,50102 CfSt.) oxn
10% Bentone `temperature
w3a4san25d070C%.,80/a9n0d
mtehseh dAentaekctroorm
Pt.eAm.perTahteureinj3e00cgGi.on
pThoert
ctoolurminsetetompe18r0agurC.e waats8prCo.grapmomremdint.o,hoalndd tfoorho4lmdina.t 1a8t075C.C.,The
flow rate was adjusted to 35 ml/min, of Argon/methane, 95/5.
MCeotFh;yolCaOt0iHonssolwuteiroen,per#5for8meTdefbeyrenacdedicnogmpaou20nd4t1oaelaicqhuotsamopflea. 1 ug/ml bsfaamip8lensetwhearnee wtahsenthleonosealdydedcapupnetdi,l aswiyerllleodw acnodloarllpoewresdisttoeds.tandThefor c15olomrinudtiessa.ppeaNrietdr,ogeanndwatshe blsoawmnpleovwears threetusranmpeldesto unittisl otrhieginyaelllow Volume with ethyl acetate.
RESULTS AND DISCUSSION
Fluoride Release
FInC-1a4ll3 wbauts mtohneitfoirnaeld gonrloywthbypearniaoldy,sisdeogfradfaltuioornideofcoFCn-c9e5ntraantdion atthattheifbetgheinnfilnugoraoncdheemincdalofpoeratchioncsulotfurethepsereiomdo.lecuIlteswasweraessumed tdheagtradfeldu,orifdleuowraisdenoiton lwoosutldfroamccuthmeulactueltuirnethbey amebdsioar.ptioTno, enpsruerceip softatfilounoriodre.volTahtiislizfaltuioorni,decocnotnrcoelntrcautlitounresiswearpeprogxriowmnatweliythwh1a5tmg/l wpoeurlcdentresfullutoriidfeFCr-el9e5asoer. FC-T1h4e3reusnudletrswenotf tdheegrafdlautoiroindewiatnhalySsOes wcaosndudcuetedtoonthedifvfaerrieanbtledaaynsd svheroywedslcuogngsiisdherraebslpeonsvearioaftitohne. flTuhoirside eTlAeBcLtEro4dbe.shoTwAsBLtEhe4aresshuolwtss tohbetarienseudltwsheonbtLahieneSdamaet seaamcphletsr,anwshfiecrh. haafdtebreetnhe sttoerremdinaintipoonlyoefthtyhleeneexpceornimteanitn.ers,Dewsepriete anthaelyzveadriatboigleitthyer diuferteoleatsheedantaolytthiecamleditaechtnhirqouueg,h btiheodergersaudlattsioinn,diwcoauteldtnhaott bfeluolroisdte, from the media.
TchuelturreessultasndofcontthreolfsluoarriedeshoawnnalyisnisTABoLnE f5l.uorTohcearbroens-ulctosntasihnoiwngthat no biodegradation with fluoride release occurred.
03643
-10-
Transfer 1 2 3 4
TABLE 4a
INITIAL AND FINAL FLUORIDE CONCENTRATION
(MmgE/A1S)UROEFD TBFHYLEUSOPTREIIDMCEEIFOSIFUCPTPIRLONAENMSEELFNEETCRETDROCDOENTRATOLS
#
FluorFiCd-e05Control Initial Fisal
TFluaorliFdete---1C4io3Pnaitnrlaoll
21
23
23
22
20
21
2
20
20
22
26
ans
18
zn
25
6.5
5 10.2
16
1.3
TABLE 4b INITIAL AND PINAL FLUORIDE CONCENTRATION (MmEgMA/E1S)AUSROUERFDECDFLOBULYOLREISCDPTEEICVIESLFUYIPCPALT1E0ME8ENNDETLEEODCFTCRSOOTNDUTEDR.YOLS
Transfer #
Fluorisdce-95Control FiInitial ael
TFluaoriiFdote---1C4ioF3niatnraloll
o1
116..64
1166..22
115..77
1157..00
23
115..26 1166..22
164..45
1165..46
4 5
1.6 15.7 1.3 1.0
15.7 17.0 15.0 16.4
03644
oe
BLE 5 INITIAL AND FINAL FLUORIDE CONCENTRATION E(OmEgF/1U)RESOFA3N4FD3C-OC14OF3NTNROAONNLDSUCPFUPCLL-TE9UM5RE-ECNSOTNETDAI95NINAGND
FC-95 Test
Transfe#r Init. Final 0.46. 0.51
1
0.50 0.460
2 0.42 0.66
3() 175 16
4 0.73 0.71
5 0.72 0.78
6 0.73 0.8
7 0.14 017
8 0.9 0.84
9 0.8 0.73
10
0.72 0.81
un 0.81 0.80
12 0.74 0.73
13
0.73 0.84
1 0.81 0.78
95 Control
FC-143 Test
143 Control
Init. Final Init. Final Init. Final
0.31 0.33 0.1 0.1 <0.1 <0.1
0.36 0.36 <0.1 <0.1
0.34 0.58 <0.1 <0.1
1.75 15.83 1 0.68 0.60 <0.1 0.1 0.61 0.68 0.1 <0.1 0.63 0.70 <0.1 0.1 @.1 1 0.1 0.1
0.66 0.68 <0.1 0.1 0.72 0.60 <.1 0.1
<0.1 <0.1
<0.1 0.1
81 0.1 <0.1 0.1 0.56 <0.1 <0.1 .1 <0.1
0.1 <.1 0.1 <1
0.60 0.68 <0.1 <0.1 <0.1 <0.1
0.69 0.62 0.1 0.1 0.1 <0.1 0.68 0.62 0.1 0.1 0.1 <0.1
0.64 0.66 <0.1 <0.1 <0.1 <0.1
0.66 0.64 0.1 <0.1 0.1 <0.1
03645
12
Reference Compounds TSRooesmfteewrhecanotcnedirtecisooimnspstoaunnutdssedtowewroderegeruassdueaidttiatobonl.edemtoonsdtergartaede thcaotmpotuhnedsbiKondoewgnrada0tiboen Icnulttuheresfiwrhsitchfowuorregroiwdtehntipcearliodtso, th3e0 mfge/slt pchuelntuorlesw,asexacdedpetd ttohattwo mpteehreaiysoudrlsea.mceknetdInofflthupeohreofcnoaourlrbtohcnosng.creonwttAhrnaatplieyortniiocdad,lurippnhrgeonboltlehmeswafspirrseftvoeunnttdherdetoothGdereogwrtahde taop0p7lliteehsdes. `tbhiaTonhdies1g.r3daedmmagot/nilos,niroatftheedphleitnmhoialtt. otfhesetensstiticvointdyitiofonsthweermeethSouditaasble In the fifth through fiial growth periods, reference linear alkyl
sulfonate (LAS) was used as the reference compound. This compound is a standard reference material used in the Soap and Detergent
ATtthsehsiestso,c`Simoaatattpheieroainnra'delssDuelbittisseordcgeaoegrnnrestaidcdaAoetsnrisseooidncdeitraotetedibseotni'mrnseevltaahlstoihidadkveefilfoyrfltaeahesanksiiorlnebyimicoovddaseeluggrrrafaodfadecadttl.ai-nodtnosdI(ne6c)e.nederived LAS is not nearly complete: The data showing the extent of degradation of LAS in surfactant LstcAuhoSepnptrledoeqelmugsiervanadtnlaeoetdtnitoscnounpaotpmcorlcuoenulntrsernetaodfereddmwuedirtetihphnyiglceLtnAeteSdhebailnrfueieTrAsdtBeaLcpEtificev6wt.eeaddsauTiphbnetsitTvaAdenBacLtEeatsrsa7.hnisonfweiLirnihsegt.tle TtthorraedneesgfrotarrsdaenwsefiernresthreweeqrsueuirrferadecqtufaionrrtedLAsSbuepfdpoelrgeermaedntahteteidomnacjoonirtnirtotylheoffo1r43tFhOec-o0Ln5At,Solb.eFgiavne LTdAheSerrievdfeeodgrreaL,dAaStiiwtoenra.eppneoTathreedinttiehsttaitalclooynrdgiaptnriieossnmesatalclainpoawbseludefffioofcriedennetrgircnahudmmiebnnegtrsio-fdfoordecene-
these organisms, but enrichment occurred at a slower rate than had
obt3feoe1n0di,en4agc-nrr6taeidacisdiaenpygsat,thteehdea.nrdaftleutoeramConpocdenhsreealmqtiiuukecrenaeltlilswhy.io,sodchGraroanofigwsetaeshcdcwlpIeierrmroeaimtoridmosnaogdmweeorrtiegneamneptixehstermeastnpudrreocedcapeadfburlroeem d(<e2g0radattoio2n25Ci)n ttoheafcionanlstagnrtowtthemppeerriaotudreareofsh2o5wCn. inReTsAuBLlEts8.of LAS wtIanhse thddeoengegrradotawottihdoentpeerromfiiondLeAsS.fifolTlho5ew0isnemggf/letsruaolnftssfFeCra-sv9e511sohroawnFsdC-11i2n4,3TAaiBnnLhEeixbp9i,etreidmFeCn-t95 CoaofpmppeLaaSrr.sisotnHoowwheiavtveher,TaAnBiLtiEsnShpirb8eitsaienndngce6ewsfahfsoewcstnototnhactotmheptlhemetiecplrryoebsieiannlcheibdioeffgorr5ay0d.amtgi/oln oootnffestoFLuACrSp-e9r5mdieeotgidhrnsoah.ddi,abtiiOtFoneCnd-.1tLh4eA3Sdoitddheegmrroatdhaantsdip,oenawribtyhfio1n8%hathvaeendla2is3mi%itgsndiuofrfiicnagtnhtetheepsfrefeeccittswioon
03646
-13-
aInndthFeC-1f4i3nalwergerowutshedpearsiotde,st 50sumbgs/tlraotfescaribnonplalc4e-loafbeltehed
FC-95 nonlabeled
ftlruioprloicchateem.icalTsh.e coBnotchentFrCa-t9i5onasndofFC-t1h4e3racudlitouarcetsivweerfeluporreopcaarrbeodnsin
parreesesnhtownin inthTeABaLqEueo1u0.s phThaese iansitidaelteFrCm-i9n5edcboyncsecnitnrtaitliloantiiosn muccohunting
slyoswteermatthiacn eerxrpoercteidn. theThicsollleocwtivoanlueofcothueldihnaivteialreFsCu-l9t5edsafmrpolmesa.
cIutltiusreaslswohepnosstiheblefirtshtat saFmCp-l9e5
had was
ntaoktenc,ompbultetetlhyis dsiesesmoslveudnliikneltyh,e
scuilntcueretsheweirneitiaallmosvtaluiedsentfiocralFC-(9350.3c,onc2e9n,t8raatnidon30.f4rommg/all)l. 3
parallel Never-
wtihtehlesFsC,-95theandreFmCa-i1n4i3ngredamtaainsehdowin tshoatluttihoenradduiroiancgtitvhietyentaisrseociated
s7h-odawyeddseogmreadabtiinodnintgesotf preardiiooda.ctivAenamlaytseirsiaolf, tbhuetbitohleogviacsatl masjoolriidtsy
remained in the liquid phase.
Transfer 4 5 6 7 8 10 1 12 13 14
TABLE 6
CONCENTRATION OF CONTROLS
LAS AND
%(mLgA/S1)REINMOSVEUDPPLEMENTED
95 - Surfactant Control
TC Ias
143 - Surfactant Control
To
1as
# Init. Final % Removal(?) Init. Final % Removal
31.5 26.8 18.4
35.5 20.5 19.4
28.3 27.5 0.1
32.8 25.8 21.2
29.8 "25.5 15.5
27.0 24.0
7.0
25.0 12.0 91.1
25.0 30.6 -2.0
3.2 3.75 89.8
37.0 35.8 11.1
33.0 3.17 95.1
38.9 13.7 94.6
32.7 2.33 95.9
42.7 12.8 89.7
31.0 2.0 95.0
30 13.7 93.8
31.3 2.33 96.5
41.3 19.7 77.9
31.7 2.5 93.5
41.3 18.0 88.3
3.3 3.0 92.8
40.3 13.7 20.7
03647
uae
BLE 7 CONCENTRATION OF METHYLENE BLUE ACTIVE SUBSTANCE
(mg/1) IN NONSUPPLEMENTED CONTROLS
Transter # 4 5 6 7
95 = Control Init. Final 10 1e 115 La 0.50.75
5.25 10.2
143 = control Init. Fisal
45 as 4.5 as 7.1 s.s0 5.0 10.2
8
3.0
0.88
92.0
10.9
9
5.75 1.83
10.9
12.2
10
4.17
1.17
12.3
9.67
u
4.33 0.67 ws 12.0
12
3.0
1.33
12.3
13.3
13
3.67 0.67 ms as
1
3.67 1.0 1s 1s
TABLE 8 COSNUCPEPNLTERMAEDTNUITROEINDNGOAFFNIDMNBNAAOLSNGSRU(OPmWBgT/L1EH)MPEENIRNTIESODUDCROFNATCRTOALNST
Time SrFuIipLpClA-.SesS#cu3opnpLltA.rSolSsWupopnl-. Initial 28.7 20.3 1.0 pay2z 13.0 26.0 1.0 = bay 7 t1.o67 0 2.0 7FP %ReLmAoSval (7)96.5 95.4
SFuippLlA.s_FC-143C#So5unptpLrlAo.Sls SFupopnls. 3.0 38.7 6.5 8.0 223 5.3 &6.83 86.&33 44.00 ole 92.3 -
03648
-15-
TABLE 9 EFBFIEOCTDEGORFADFACT-I9O5NAONFD FLACS-14AN3ALONYZTEHDE
FOR AS MBAS
TFo C-05 + LAS wCuluturse g FC-14l 3 + a LAS Cs ultus re Transfer # Init. Final Removal(8) Init. Final Removal(s)
1
58.0 34.7 713.6 83.7 27.3 97.8
12
64.3 40.3 78.9 53.7 34.0 714
TABLE 10 THCEONCCEENNTTRRIAFTUIGOANTEOOFF*%TEcS-TrcC-U0L5TUoRrE 1D4UcR-IrNcG-143 IN
THE FINAL GROWTH PERIOD
Init. Day 2 Day 7
TST4tc-rac-9n5 d cultaurers d ~ Mo-r~ c-143 culSttuarnedsard Concentration Deviation Concentration Deviation
30.1 mg/l 52.8 53.5
0.3 mg/1 0.5 3.1
46.2 mg/1 48.0 49.7
0.9 mg/1 0.3 0.4
03649
"16-
Tachtiinv-elamyeetrabcohlriocmaptroogdruacpthsy 835 1iquid chromatography
oodiffdetinhoetthesrarmeeFveOa-cl1u4l3ttuhsoeerpFsrCxe-is0ee8a.n.sctes.bofikbeermraesdaico,-
Bicenofimotbriieanlaltayinodnpraoefsfteenrtthemoseerthynrloeatstuilaotlnss,,o pSitrheoswceaenndtnbeoincProcoonndctuircuoeglsesd.tthheFartroWmemroesmhohoomt
degradation of these fluorochemicals cecurred.
REFERENCES AND FOOTNOTES (1) Goldman, Peter, Enzymology of Carbon-Halogen Bonds.
RDeegeradeataionoTof eSkynthetWaischiOnrpgioann,icDOMolTecouylEes in tthheeBiBioossphheerree,
(2) Tasthreorw25etywaCp.seriiaondsitdhxeu-rdipanrygespweenhrciiecohdofbtehfeFoCr-te9e5sttohercuolFntCs-u1er4te3s.owferteheshfaikneasl (3)TdStthandardEdMlteiotnh, oAdmserfiocrantPheubElxiacmHienaalttihon ofResWoaotieeriiosnnd Wastew(T87SIoarteetr:, (4) D22agtiF.m"e Ntiegmhpteratteumrpeesratwuerreescwbesreervneodttmoenrsaungreedbedtuwreienng 2t0haranpdart
oentfiregarhtmttuihtrneieantseit(ousndetyeeomfTpinAeBrtLwahEhitsiucr3he)2.4-cfmuroHlenotqtwuuherevenestsrlt,yuwdeyrmd,reeoapssishunarketJemoanenun1at7ag5tym,Caa.dmebsihenonewtsetdtetimhnpa-t (5) Aathitghhis flturoarnsifdeer,coDnececnattruartisolnu.dge was added which contained (6) Subcomnitree on Biodegradation Test Methods of the Soap and
DDSeeuttleofrrogmneiannttaetiaAosnnsdocLoifiantetiahoren,bAlikoAydPleargtorecaeddaSuburilelfiotanynadtoefStaAlnJ.kdyalrodfsBtehfneosroAnmtehreican
0il Chemists" Society, 42:986 (1966).
(7) Percent LAS removal was calculated as:
% Removal = (MBASog,er"- MMOBAASSe.y;))--(M(MBBAASSge,y --MMBBAASSc.e)) oo
MBASG; - MBAS;
Where: MBASG; = Tchoenceinntirtaiatlionmetohfyltehenesubrlfuaectaacnttivseupspulbesmtaenncceesd w(uMlBtAuSs)e. MBASG; = Tchoentrionliti(aTlABLMEBAS7)concentration of the nonsupplemented MBASgy = sTuhepplfeimnealnteWdBAoSulcotunrcee.ntration of the surfactant MBASG; = Tchoentrfoilnal(TMABBLAES 7c)o.ncentration of the nonsupplemented
03650
i
(850000000 1%)
!od
(7) The percent LAS Removal was calculated as:
Cont
(MBASgyy - MBAS;;) - (MBASgr, - MBASp)
% Removal = -------- (M-- BAS; =- MBA--SG;)
where:
-- X 100
MBASgp;
=
LAS Buractant and oioher FO-55 br Petes. The initial methylene blue active substances
concentration of the culture supplemented by
(MBAS) both
MBASG;
= The initial MBAS concentration
Plementalcontrol (TABLE 7).
of
the
monsup-
MBASgry = TShuepplfeimneanlteMdBAwSitchoneceunrtfraacttiaonn osfadcuflltiuorrecsarbon.
MBASCp = The final MBAS concentration of the nonsupplemental control (TABLE 7).
MBASg, = The initial MBAS concentration of the surfactant
oFIFCt-v1ta4hs3esevsasssSsuuobpnmeopedtloemrtneheenad:ttuacleMdBAcSbuyltcuotrhneecebn(itTorAdaBetLEgiorna6)d.adtuieontoorFCs-n5esorloss
ftfo
Shnjcen
03651
