Document oDyXK07xKovOyJdor1YYrBmZ3

Copies to: E. I. du Pont d-; Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine HASKELL LABORATORY REPORT NO. 23^-73 Haskell No.: 7942 AR226-2835 PRIMARY SKIN IRRITATION AND SENSITIZATION TESTS ON GUINEA PIGS Procedure; The test for primary irritation was conducted by applying, and lightly rubbing in, of a 257, and 10% solution, (wt/vol) of the test material in distilled water on the shaved intac of 10 male albino guinea pigs. To test for the sensitization potential, a series of four sacr injections was given, one each week over a three-week period, which consisted of 0.1 ml of a 1 of test material in distilled water. Following a two-week rest period, the test animals were sensitization by applying, and lightly rubbing in, 1 drop (ruO.05 ml) of a 25% and 10% solution material in distilled water on the shaved intact shoulder skin. A group of '10 previously unex received similar applications at the time of challenge to provide a direct comparison of the c on skin of similar age. Results: Reactions* on Inltact Guinea Pig Skin Test Areas (10 Animals - In. Avg. Wt. = 467 g Final Avg. We. = 718 g) Control Areas (10 New Animals Concentration in distilled water 25% 10% 25% Primary Irritation Test 24 Hours 10 Neg. lu Neg. 48 Hours 10 Neg. 10 Neg. Challenge Test 24 Hours 48 Hourr 10 Neg. 10 Neg. 10 Neg. 10 Neg. 10 Neg. 10 Neg. Number of AnimalsSensitized** 0/10 .Summary: yjlJ^HBl produced no irritation or sensitization when tested on guinea pig skin at 25% and ITO in distilled water. Company Sanitized - 2 - * Reaction Code: +, -H-, +++ = mild, moderate strong erythema; I'll I = erythema plus edema: ++ ** Sensitlzation is defined as a two-step score increase (++) at challenge over the response ex same amount applied initially or on the concurrent controls. m E3281 p. 50 OLD/ smw Date: May 8, 1973 Report by: 0. Louis Dashiell Approved by:____C/tg^i^e^ ^~. /\Ce<-^r^F Charles F. Reinhard.t Assistant Director QwPUV Sanitized