Document oDVm6jLp5wQ7E7ZM4mmeQdqRD

MICROBICS MICROTOX@) TOXICITY TEST TEST SUBSTANCE Identity:Perfluorooctanoiaccid,ammonium salt;may also be referredto as PFOA ammonium salt,Ammonium pernuorooctanoate,PFO, FC-1 16, FC-1 26, FC-1 69, FC-1 43, or as a major component of FX-1003. (Octanoicacid,pentadecafluoro-a,mmonium salt, CAS # 3825-26-1) Remarks: The 3M productionlotnumber was 2327. The testsample isFX-1 003. It'psuritywas not sufficientclhyaracterized, though currentinformationindicatesitisa solutionof <45% ammonium perfluomoctanoate,50% water,<3% inertperfluodnatedcompound and 1 - 2% C5 and C7 perfluof;aonalogue compounds. The followingsummary applies to the testsample as a mixture of the testsubstance in water solutionwith incompletely characterized concentratfons of impurities.Data may not accurately relatetoxicity of the testsample with thatof the testsubstance. METHOD: Method: MicrobicsMicrotox@"BASIC" Procedure Test type:Staticacute GLP: No Year Completed: 1990 Species: Photobacteriumphosphoreum Analyticalmonitoring:Lightloss. Exposure period:30-minutes Statisticamlethods: EC,o and EC5ovaluescalculatebdy statisticlailnear regressionprogram providedforMicrotox@) Test organism source: Not noted. Test conditions: Diluent:2% NaCl Microbic'sReagent. Test temperature: Not noted. Stock and testsolutionpreparation:A 2000 mg/L stocksolutionwas prepared inMilliporMeilli-Qw*ater. Test solutionswere prepared using aliquotsof the stock solution. Concentrations dosing rate:Once Stabilityof the testchemical solutions:Not noted Exposure vessels: Microbic'csuveftes. Number of replicates:two Number of concentrations:Four plusa blank control RESULTS Nominal concentrations:Blank control1,25,250, 500, and 1000 mg/L Element value: 5-minute EC50 = >1,000 mg/L 15-minute ECso = >1,000 mg/L 30-minute ECro = >1,000 mg/L Allelement values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureofthe testsubstance as describedinthe testsubstance remarks field.The values reportedapply tothatmixtureand notthe testsubstance. CONCLUSIONS The FX-1003 30-minute EC5o forPhotobacteriumphosphoreum was determined tobe >1,000 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking2. Testingmeets allcriterifaorquality testingb,ut lacksanalyticaclonfirmatioonftestsubstance concentrations. There isa lackofcharacterizatioonfthe testsample. REFERENCES This study was conducted by the 3M EnvironmentalLaboratory,St.Paul, Minnesota,Lab Request number G2882. OTHER Last changed: 5/25/00 ENVIRONMENTALLABORATORY MITROBICS MICROTOXR SYSTEM RAW DATA P8 LR K-1 TEST SUBSTANCE: Y/-e)), :z TEST DATE ANALYST: SAMPLE CUVETTE FINAL ASSAY FONCENTRATION L; INITIAL READING 1(0) FINAL READINGS 5-MIN. 15-MIN. 30-MIN. 1(15) 1(30) BLANK CONTROL ci DUPLICATE 77 (PS 49 /0 7,3 -73 B2 I.A -,57 log 79 C2 79 A B3 10q 76 lo 5-9 C3 U-P tca4 e 105- 7,Y loo 5& .B4 76 &,3 Li0o C4 i )06 71/ 60 '516 B5 )og qq Ll6 C5 -1U@PI@I"CC.S,4e /09 7 5@P, q ct le,Z!A9v@>SkCLV,.C9e4,ock A ooo ry4!I@L 1:" 1.0 rKk TessiSrubs-4ance 'XPPcLfer4tnYtsct-61wei.A,-W AA# lii'C-L L4jc.L4er. Pffz (o8. ) O-Ai v%*cL +0 1>* & .-7 1 S.Pt, c AA v4-tov%S n @'4'CO@L(OviCen4 t-cl+oiK uj eire-ctov6 t-e- '!@4,y C04cgit-@r4.+ico@-. PYLer14ion,-ct elbove. -rlvtklkaadi'lcA tons L'L4rf-PlAc,'A, +r' Microtox 5-min exP MICROTOX(R) DATA SHEET FX-1003 LR 62882 3/7/90 sab PAIR CONC. i 125. Tjo-(.) 2 125. C)OO 3 250.oOO 4 250.000 5 500.oOO .5 50C). OOC) 7 %looo.ooo i3 %1000 .000 BLANK Bo/Bt= too BLANK RATIO= O.BlOo Io/It 109.0/ 79.0 lOt3.o/ 78.0 109.0/ 76.0 105.0/ 74.0 111.0/ 76.0 106. ('-'7)4/.0 108.0/ 65.0 109.0/ 67.0 el B-OBS 0. 1 if3 0.122 0.162 0.149 0.1(33 0.160 0.346 0.318 G-EST 0.112 0.112 0.154 0.154 0.211 0.211 0.2eg 0.289 oee + it it 1-4 .2(0 ;@-'C5C) %9347. 118 R =0.93295 SLOPE %2565.755 TO %34051.820 1.9111 INTERCEPT +9.1428 c50 > IC)C)C>IL ---------------- RES. PROB. i 1 0.420 57 2 0.402 53 3 ().290 27 4 0.311 31 5 0.279 25 6 0.292 28 -1 > 7 (:).555 too 8 0.502 E32 ---------------- L C) < 7 ---------------- A --------------- m ---------- m 3 --------- 6 -2 ---------- ------------------------------ A <s ----------------- ------------- ----------------^ --------------- ---------------- 4 5 LOS CONC. 6 7 MICROTOX is a Registered Trade Mark of Microbics Corporation. Micratox rllCROTOX(r-) DATA SHEET 15-min exp. FX-100:3 LR 62882 3/7/90 sab PAIR CONC. 1 125.000 2 125.000 3 250.000 4 250.000 5 500.000 6 5oo.000 7 %looo.ooo 8 %1000.000 BLANK Bo/Bt= 100. BLANK RATIO= 0.7100 lo/It 109.0/ 64.0 10e.O/ 66.0 109.0/ 64.0 105.0/ 60.o 111.0/ 63.0 106.0/ 60.0 108.0/ 49.0 109.0/ 52.0 71 G-OBS 0.209 0.162 0.209 0.242 C).251 0.254 0.565 0.488 G-EST 0.167 0.167 C).231 0.231 C).321 0.321 0.444 0.444 P%3 17 14 1,7 , I-A .90 AO 3(0 33 EC 50 %33 47.798 %1084. 118-TO %10338.120 R =().90172 'SLOPE 1.7282 INTERCEF,T +8.1161 EC!Se.>> 1000 m RES. PROB. 1 0.400 33 2 0.546 62 3 0.400 33 4 0.348 25 5 0.341 24 6 0.339 24 7 0.691 100 a 0.588 72 ---------------v---------------i L 0> 0 <6 G A 7 M m 8-. A -------------<- s 4 6 ----------3 ---------------------------- ------------2 --------- -2 ------------------------------------------------- 4 5 LDS CONC. 6 MICROTOX is a Registered Trade Mark of Microbics Corporation. MICROTOX(R) DATA SHEET Microtax 30-min exp. FX-100--o LR 62832 3/7/90 sab PIS q PAIR CONC. 1 1 ':-@'.5rd0 0 2 125.000 3 250-000 4 250.000 5 500. OC)O 6 5oO.OOO 7 %1000.000 a %1000.000 BLANK Bo/Bt= 100 BLANK RATIO= 0.71OU EC 50 %2918.197 R =0.91498 SLOPE lo/It 109.0/ 62.0 108.0/ 62.0 109.0/ 59.0 105.0/ 56.0 111.0/ 60.0 106.0/ 56.(-) 108.0/ 46.0 li7.)9.04/9. (1 71 -- - G-oss 0.248 0.237 0.312 0.331 0. 31 -@; 0.344 0.667 0.579 %1094.210 TO %7782.670 2.0386 INTERCEPT +7.9787 G-EST 0.232 0.232 0. 3OE3 0.308 0.409 0.409 0.544 0.544 )4(. EC C- Li ,5- 37 x too RES. PROB. 1 0. 448 48 2 0.480 55 3 o.334 27 4 0.319 24 5 0.332 26 6 0.314 24 7 0.647 100 8 0.539 70 ---------------- L 0 0> <G 7 A a m ------------- m ----------------- A ---------------- <s -4 ----------6- --3 5 ------------- ------------2 -------- ------------------ -2 ---------------------------------------------------- 4 5 LOG CONC. 6 7 MICROTOX is a Reqistered Trade Mark -@o-Mficrobics Corporation.