Document oD7xYqVQZO55QNJgp26N11MLE

The Toxicity of the.Fogs Formed by Dropping Pydraui'F-9< Aroclor IP^S and Trlcresyl Phosphate, Upon the Surface of a Heated Inconel (Project ^9) by Treon, Cleveland, Shaffer, Cappel, oahegan, Wagner March 11, 1953 055^81 HONS , /) " Page I SOOpe ............................................................................................ 1 Conclusion* . ........................................................................ 1 Matorlala ................................................................ ..... $ Experimental Method* ....................................................... , 7 Equipment.............................. .... ..................................... Volatilisation of the Material* Without Pooong>osltlon .............. 7 7 Generation of a Peg Prom a Heated inoonel Surfao* . ......................... 9 Sampling and Analysla of Contaminated Air . , 11 uxperlmontal Result# ....................................................... .... 19 Exposure to TJndeoomposad Vapor*..........................19 Exposure to Producta Arising Pro* fydraul p-9, Aroolor 1248. or Trloraayl Phosphate When In Contaot with Inoonel at 1250 or 10J>00 F.................................... ......................................... . 21 Mortality .......................................................................... 21 Gif;ns and Pathology of Intoxloatlon......................23 The Conoentratlona of Idantlflad Deoompoaltiea Produota Formed at tba Higher and Lower Temperature# .................................. ..... 2$ Olsousslon . . ................................................. Supplementary Statement * She Applleatlen of Pydraul F-9* Aroolor 1246 and Xrleresyl phosphate Open the Skin of Itabblta.................................. .... 31 Experimental Methods Experimental Rssuite 31 3 . HONS 055982 ] 28 ,conf,.DENt,al Seouenoe of Table* 1. Tha Mortality, Length of Survival, and Change* In Wolght of Animal* ixpooed to the vapor of f/draul K-9 In Air. 2. The Mortality, length of urvlvel, und Changes In Weight of Animals v.xpoeod to tho Vapor of Aroolor 1240 In Air. 3. Tho Mortality, Longth of Survival, ond chan'.;** In Weight of Animals wxposod to tho Vnpor of frlorosyl Phosphate In Air. 1|.. Concentrations of Various DaooTnpooHlon Products in Air of Chiuobor In tho sovoral tjtperlmont*. $t tho Mortality, Longth of Survival, and Chanda* In Volght of Animals dxpoood to Aerosol* Formed by Dropping Pydraul F-9 Into an Inconel Tube lloatod to a romporatu.ro of I2i>0 F. (677 0.). 6. l'ho Mortality, Longth of Survival, and Change* In Weight of Animals .Ixpoaod to Aerosol* Formed by Dropping Pydraul F-9 Into ary Inoonsl Tub* Hoatod to a i'onporature of 101*0 F, (;i66 C.). 7. The Mortality, Longth of Survival, and Change* In height of Animal* ijtpoBOd to Aororolo Formed by Dropping Aroolor 11l|B into an Inoonol lubu . oatod to a Tomperature of Xe.'JO0 f. (077 c.). 0. Tho Mortality, length of Survival, and Chani-e* In Weight of Animals ..xpceod to Aerosol* Foruod by Dropping Aroolor l?lj0 Into an Inoonol Tube iloatod to a i'omperaturo of 1030 F. (506 0.). 9, Tho ''ortality, Length of Survival, and Change* in '.,'olt.ht of Animal* .exposed to Aerosols formed by Dropping Trloreeyl 1'hoephato Into an Inoonol Tub* hoatod to a Temperature of 12^0 F, (677 C.). 10. Tho Mortality, Length of Survival, and Chang** In Wolght of Animal* imposed to Aerosol* i ormed by Dropping Trloroayl Phosphate Into on Inoonol 'Tube heated to a Temperature of 10J>0 F. (5>'66 C.J. HONS 055983 ?PNF/DfcN-TML 11. busmry ot Mortality Among Groupa of Aninal* Mubjeotod to Aerosols formed by Dropping iydraul F-9, Aroolor 1248 or frlorosyl Phosphate Into a Heated lnoonol Tube. 12. J.noldonoe of Mortality In Relation to Matorlal and Gpooloa Regardless of tho Tenvoratura of th* lnoonol, 13. inoldonoo of Mortality In Relation to Material, Rpooloa, and Temperature of lnoonol. l!|.. The Toxlolty of i'ydraul 1-9 When Maintained Continuously for Twenty-four ilours In Oontaot With tho Skin of Rabblte by tho floovo Method of ih-nlso, Woodard and Calvary. lj>. Tho Toxlolty of Aroolor 1?1|0 When :;alntalned Continuously for Twenty-four Hours in Contaot vath tho akin of Hobbit* by tho Sleeve Method of Dralse, Woodard and Colvery. 16. The Toxlolty of Trloreoyl Phosphate When Maintained Continuously Tor Twonty-four Hours In Contaot v/lth the olein of Rabbits by tho nioovo Method of Dralse, Woodard and Colvery. 17. Tho Toxlolty of Gargoyle DTK When Maintained Continuously for Twenty-four Hours In Contaot with tho bkln of Rabbits by the f.loevo :!othod of .'raise, Woodard and Calvory. . 1C. Bujnarlied Data on the Immediate Toxlolty of Pydraul ''-9, Aroolor 1248, frlorosyl phosphate and Gargoyle DIB When ulntalned Upon th* ; kin of rabbit* Aooordlnc to tho Mloeve Method of braise, Woodard and Calvary. I I | ; 1 ] | ] j i ! | j ! HONS 055989 `CONFIDENTIAL 1, Hoated Veil Froa Willoh Pydraul F-9 Woo Bveporatod Into Air Otreama. 2, Tower* From Whloh I.lquld* ore Volatilised. 3. Equipment for tho '.'onoration of the Decomposition Jroduota of llydraullo Fluids at High Tomporaturee. 4. Absorption Train for Collection of Carbon Jlonoxlda From Air* J>. Standardisation Curve for the Determination of Fydraul< . 6* Standardisation Curvo for the Determination of Aroolor 1248* 7, standardisation Curve for the Determination of Trloreayl Phosphate. 6. standardisation Curve for the Determination of Hodlwf Chloride. 9. Standardisation Curve for tho Determination of Eata-Creaol. 10. Standardisation Curve for the Determination of lara-Oreaol. 11. Schematic Arrangement of Equipment shown In Figure 3. HONS 055985 #.Qto7-ir The Toxlolty of the Foge Formed by Dropping Pydraul F-9, Aroolor 1846 and Trloresyl Phosphate, Upon the Surfaoe of a Heated Inoonel (Vrojeot 49) Soope The purposes of this Investigation v/ere to determine the Immediate toxlolty of Pydraul F-9 and two other hydraullo fluids, Aroolor 1846 and trloresyl phosphate, (1) when vaporized at moderately elevated temperatures ond {?.) when Inhaled, by animals of several speoles, as fogs formed by dx'opplng them upon Inoonel, maintained at temperatures of approximately 1850* and 1050* F. (677* and 566 0.), these toraperatures being, respectively, sonowhat above ond somewhat below the fire point. A nupplemontary statement oonoerning the Immediate toxlolty of theae materials when maintained In oontaot with the skin of rabbits Is inoluded at the end of this report. Conclusions MONS 055986 1. Animals of several speoles survived without signs of lntoxloation when exposed to air laden with Pydraul F-9, Aroolor 1246 or trloresyl phosphate for 7 hours per CONf ,DeN TIal 2 day on enoli of several days, i'he concentration* of the notorial* In the air end tho number* of day* of o.xpo*ur* vor* os follows) lydraul K9 3.0 mloroi\r*m por liter, y days) Aroolor 1240, 431 Mlorotramn per lltor, 4 day*) and Lrlororyl phoaphate, 01 > nloroy.ram* per lltor, 0 dnya. 2, fho Inhalation of vapor* of fydraul i'9, /roclor 1M|.'; and trlcreeyl piioaphale formed at riodorotely olovatod 1'oora to .poroturos lnduoed minor obcii;,so In tho 1vo:o ol' the animal* .espiratory exposure to either fyuruul < 9 or trleroeyl phosphate induood ^odorate lneroaso* In th.* in-bore of hietlooytoe within tho lunt: p*ronoiiy-a and the air parra e* of oats, whllo eorrerpondln& exposure of other species to either lydroul F-9 or Aroolor 1240 induoed Intoratltlal pneumonltl* and apparently contributed to the severity of this lesion a* It 1 observed, not infrequently, In control animals. Intercurront lnfoetlon, uru'olatod to tho experimental prooedure, obaourod Interpretation of the morbid chan,"os In tho tissue* of r i ulnoa pip and 2 rsbbltn opposed to tho vapor of trlorosyl phoph*to, on* of whloh dlod 4 dsy* after the last porlod of exposure, i'he vlcoora of tho rate and riloe were normal. babbit l' 907s tho only animal of tho <roup exposed to Aroolor 1240 from whloh tissue* were subjooted to mlcrosoople examination, had fatty degeneration and neoroala of the HONS 055987 % lt> ^'4 4' 3 hepatio parenohyma. Other animal* that were exposed to vapors from Pydraul F-9 Aroolor 124$ or trioresyl phos phate exhibited no histologlo alterations of the viscera, aside from the lung, whioh oould be aserlbed to the toxle notion of the materials under investigation, 3. The foge of these materials arising from oontaot with Jnoonel at either 1250* or 1050* F. were immediately irritating to the muoous membranes of animals. Exposure for SO minutes to the fog of Aroolor 124$ lnduoed labored respiration, but In the oase of the other materials this became evident only after exposure for almost an hour. 4. All animals survived when exposed for 15 or 20 minutes to the fogs fornsd from Pydraul F-9 or trioresyl phosphate, at either 1050 or 1250* F,, but exposure for corresponding periods of tlms to ths fog from Aroolor 124(5 resulted fatally. In general, guinea pigs and mloe died more promptly than rats and rabbits. 5. Guinea pigs and mioe suffered greater over-all mortality than did rats and rabbits when exposed to the fogs formed from any one of the three materials. Aroolor 124$ was more toxlo than either trioresyl phosphate or Pydraul F-9 Irrespective of the speolee of animals by whioh toxlolty was tested. 6. The fog of Aroolor 124$ formed by oontaot with Inoonel at 1250* F. was more toxlo then that formed when HONS 055968 Co kh - th* temperaturo vm 1050 F, l'he fog of J'ydraul F-9 appeared to bo eltghtly moro toxla when generated ot tho high#* temperature, but tho oonvoroo woo true of foe* from triorosyl phoephate. 7, halving tho rate of delivery of tho liquid upon tho hoctod rotftl tendod to loosen tho mortality induced by inhalation of a resulting foe, but thl* was not truo in all Instance*. 0. Hiorosoopio examination of tissue* from animal* that diod a* a r*ult of oxpoeure to tho fog* of lydraul F-9, Aroolor lFJpO and trloroayl phoaphate rovonlod pulmo nary lesion* characterised by intra-alvoolor odema and IsomorrhftLC, diffuse hyporomla, omphyaena and extensive deposition of carbon in tho bronohi, fboro waa also peronobynatoua degeneration of tho, liver, kidney and brain, lisruen from the animal* that survived revealed only pul onnry loolon* manifested by intcratltlal pnoulonHln and Infiltration of oarbon-laden hlstiooyto* throughout tho alveoli and bronchi, fho Ida s of animal* that wore exposed for tho hortot periods wore nonnal, ne wore those killed ?6 day* or longer after the last period of exposure. 9. fho minimum lethal doeo of undiluted fydraul F-9, when maintained in contaot with th* intact akin of rabbit* aooordlng to the Sip-hour levo nothod of Praise, MONS 055969 r.O f C> I . . -5 "A'r /-i, Woodard and Calvery, was greater than 3.6 ml. per kg. and lose than 6.0 ml. per kg., of body weight. The correspond ing value for trloreeyl phosphate was between 1.6 and 2.5 ml. per kg. Aroolor 1246 was not lethal in the dosage of 9.4 ml. par kg. Pydraul F-9 and Aroolor 1246 are more toxlo if applied to the abraded skin. Materials Pydraul F-9 is a olear, light amber, oily liquid vihioh has a not unpleasant sweet odor. Its viooosity at 100* F, and 210" F., respectively, is 56.0 and 6.0 Centistokesj its speoifio gravity at 25/5 0, is 1,266, its pour point +5* F. Trloreeyl phosphate is a colorless, odorless liquid, tho vleooslty of which at 0 end 25* 0., respectively, is 1256.14 and 66.6 Centlstokes, Its moleoular weight is 366.4, its speoifio gravity at 25*/85* 0., 1.162, its flash point 470 F. (43 0.), its crystallization point is less than -35* 0., and Its boiling point 420 C, The sample used v/os made from oresyllo aold in whloh there was less than 3 per oent of the ortho isomer. Aroolor 1246 is a straw-oolored liquid whloh has a slightly sweet odor. It is more vlsoous than trloreeyl phosphate. Aroolor 1246 Is biphenyl ohlorineted to the MOMS 055990 ^,0 6 n r^L extent of 4 per sent. It* molooular weight 1* 192, end it* density i* 1.44 P. pw ml. At 30 c. (06 P.) and 1.19 e* pe* wl. *t 300 0. (572 P). It* viscosity rang** from 112 oentlpolse* at 30 0. to 0.47 at 300 C., and It distill# between 340 end 375 C., it# vapor pres sure being 0.00037 aw., 0,16 ;,, 2.9 ran end 360.0 mm. of moroury at 37.0 0., 100 C., l'JO0 0, end 300 C., rospoolively, aooordlnf, to hoArdlo, Garrett ar.d benignua Wud, .'ng. Ohon. 41)1341. 1949). i'hose authors have quotod the National tonrd of Flro Underwriter# to the offeot that "decomposition of the product (Aroolor 1246) we* not appreciable at temperature# below 400 C. (7?2 F.) but booeme increasingly apparent at higher temperaturos," 'Die oo:>pound ha# a flash point of 193 to 196 0.. but no flro point In the Cleveland open oup, h'bon olthor lydraul f-9 or Aroolor 1248 was drop.od Into on Inoonol tube Alnta'ned at approximately 12^0 F. (677 0.) the liquid burned momentarily with a bright yellow-white or orange-whlto flame, and then burat into a dense cloud of black omoke. Muoh glossy carbon was doposltod on the etdea of the manifold loading to the ohambor, which rapidly became filled with dense prey smoke. This soon turned Jot blaok, apparently booause of the formation of flakes of carbon a# soot. At lOb'O F. (666 C,), these liquid* smouldered and burst Into a MONS 055991 .. r c0 N fi- lo -7 donHO ;roy-white foe* In whloh wore snail amounts of oarbon soot* In oil Instances tho odor v.oe norld and pungent. Vhon dropped Into tbs Inoonol tube hooted to either 121>0o or 1050 K. trlcresyl phonphoto rove rise to a oharaotor1st,lo phenolic or orosyllo odor and boluwod as did tho other two atorlals when droppod upon iiiatnl at fro hi; hor tempore tv.ro. At temperature* bolow 102rj I',, trlorosyl phosphate appeared to shoulder without flaming* j-aulpinont. the animals wore oonfinod in a ?23-lJ.tr cltiidrloal stool c'-.oibor oos tod on tho Inside aurfao* with n onked plr.atlo. Jnleee a heavy fop, was pronont tho bo. av lor of tho anl al could bo observed through a cheat of cion," pliable plastlo no -brans, whloh sealed the otherwise open f ront end of tho oluv'ibor. An Interposed heavy wlro-mesh screen prevented t'no onfnols Iron breaking tho plan tic Membrane* She oontaminated air entered tlie ohn;,bor ;;onr tho top of tho roar wall on whloh was ,-iounted a olrculatliV fan. Affluent air was wltlidrawn tl\rou;h an outlet located toward* tho front of the lower pert of the chamber by . alntalnlng a slight negative pressure. Volatilisation of tho Pateriala Without Decomposition, i'or tho volatilisation of either trlorosyl phosphate or Aroolor 1?1|8, two flet-bottomod cylindrical bubbling tower* HONS 055992 ... '"^1 CONFIDENTIAL (8.94" * 13") In parallel (Figure 2) were eivloyed, n fritted glass thlmbl* (0,94* * 3*75") being Immersed In th* liquid eontalned in eaoh. Thai* tower* war# kept In a bath of Kujol malntalnad at 109 0. Air passed through thd two bubbling tower* at rata* of .0 and 6,3 lltor* per minute, X'eepeotlvely, a* noasurod by a rotameter in ottoh line, the stream* Merging in a glass tuba leading to a manifold intwreod in a ooollng bath before entering the chamber. At tha etart of t!i experiment 550 milliliter* of either trioreeyl phoephate or Aroolor 1240 ware plnood in oaoh of the two bubbling tower*, In the case of trl- orosyl phosphate. It was found that the eonoantratlon of vapor within tha ohambcr boooraa lo on the seoond day of exposure and a till loa* on the third, It 1* thought that tho trioreeyl phoephate might have hydrolysed some what during the oouree of standing overnight, possibly from raoletur* absorbed from the air passing through It during the day. In order to maintain the oonoentrattone ne uniform a* possible, the material remaining In the towere was roplaood with a fresh supply on the fourth-day of tho oxportmont. Had sufficient material been on hand, this would have boon done daily In order to attain a somewhat higher average concentration throughout an exper iment, This illffloulty did not arlso In tho oa*e of the Aroolor 1240. HONS 055993 ' ""Ot g*!Ul! I -9* frPNF 10 C f/ TI Air It was neoeeeary to employ other equipment for the volatilization of Pydraul F-9 beoause of extensive foaming In the bubblera. Therefore, the Pydraul F-9 was maintained at temperatures ranging from 109* to 112* 0, In a glass well heated by nlohroate wire (Figure 1). Air at the rate of SO liters per minute, as measured by a rotameter, passed ovor the surfaoe of the heated liquid and than through a manifold ooollng bath before entering the Ohamber, A volume of 190 ml, of fresh Pydraul y-9 waa addod to the well dally, of whloh about 1J0 ml, remained at the end of the day. Generation of a Foa From a Heated Inoonal Surfaoe. A rotameter for measuring the flow of air, a furnaoa for volatilizing and decomposing the liquid, a eeml-potentlomoter (see below), a ooollng manifold, and the ohamber for exposing the animals are shown In Figure 3. In these experiments the smaller rotameter, to the right In the photograph, was not employed, and all air (20 liters per minute) that entered the ohamber was passed through ths furnace. The ooollng oolle were paoked in the bath shown and surrounded by orushed lot. The body of the furneoe oonslsted of a 26-lnoh length of oold-dravm, seamless Inoonel tubing, having an outside diameter of 1.5 Inohes and a wall-thloknesa of 0,049 lnoh, Pydraul K-9, trloresyl phosphate or Aroolor 1S4U HONS 055990 VC O N F |.D G N T I A L 10 was droppod upon the innor surfeoo of the tub* from b burette through an Inoonol tubo weldod to the uppor eurfaoe of th heated tub* near lt midpoint, Phis side tubo tied a length of C lnchao, en outside diameter of 0,675 Inoh, ond a wall-thloknose of 0.091 Inoh. Another olooo of inconel tubing 3 Indies in length and having nn ouuaido diameter of 0,106 Inch and a wall-thlcftieaa of 0.049 Inoh, was alno weldod to tho uppor portion of the furnaoe tubo, to permit tho Insertion of a thermocouple .In such e manner os to measure tho tomperoturo at the upper Interior evrfaoe of the furnaoe. file dlstanoe from oenter to oonter of tho two smaller tubes, whfoh wore parallel to one another and perpendicular to tho furnace proper, was 1,75 Inohoe. ,he outside of tho furnace wee wrapped with 2 layers of molding mloa. fhe middle 10-inoh portion of the lotiptli of tho furnaoe was olootrloally hooted by means of 20 foot (9 wrapping* to the Inoh) of A. i:, u. Jiu.ber 20 I'ophot "0" wire, which hart a roalstonce of 0,659 ohms per foot at 60 F. The wire was Insulated with approxi mately 0.75 Inoh of dietonaoeoue earth and wrapped on the outside with cubes toe oloth, Pbo temporature of the wall was measured by an lronoonstnntan thermocouple attached to a semi-potentiometer calibrated against a student potentiometer, The thermo couple vas in oontaet with the upper part of the furnaoe HONS 055995 - 11 - cONF/DCNT'fA about 1 Inch downstream from the point at whloh the droplets of material fell upon Its lower Interior eurfaoe. After the furnaoe had been brought to the desired temperature of either 10JO* F. or 1850* F., the hydreullo fluid was dropped from a burette at the rate of 0.067 bo 0.245 ml. per minute (of. Table 4), 0.100 to O.167 ml. per minute being the usual rate. Sampling and Analysis of Oontamlnated Air. When the undeoomposed materials were metered Into the air In the ohamber, 4 samples of air eaoh day were passed from the ohamber, at the rate of 3.4 liters per minute, through 8 absorption towers (8.25" x 13" outside diameter), eaoh oontalnlng 100 ml. of an appropriate solvent. Redistilled ethyl aloohol was used in oolieotlng trloresyl phosphate or Aroolor 1848, while hexane wae used for Pydraul F-9. The sample passed through a ooarse fritted glass dlso (1.13" in diameter) Immersed in the solvent and plaoed parallel to the bottom of the tower and about O.E" above It. The oontent of Pydraul F-9, Aroolor 1248 or trloresyl phosphate 00 colleoted was determined by measuring, by means of a Beokman spootrophotometor, tho ability of the solution to transmit ultraviolet radiations of wave lengths 248 BijH, 248 tyc and 284 ty(, respectively. HONS 055996 *r - 18 - C O M 1 D - N T I A L' A ourve relating the transmission at 846 tytto the concentration of Pydraul F-9 in hexane over the range of 4.7 I 71 miorograme per ml. is shown in Figure 5A comparison of the corresponding ourvee for Aroolor (Figure 6) and trloresyl phosphate (Figure 7). are also shown. The oloohol used as solvent for Aroolor and trloresyl phosphate was freed from ultravlolet-ahsorbing impurities hy the following procedure 1 About 1750 ml. of 95 per oent ethyl aloohol were permitted to stand overnight with 10 g. of pelletB of KOH and 7 8 aluminum foil (O.OOJ"). The mixture was then refluxed for half nn hour and dlatilled at 76*50* 0., the first 80 ml. being rebooted. In experimentB involving the exposure of animals to tho produots formed on thermal decomposition of l'ydroul, Aroolor or trloresyl phosphate for no longer than 7 hours, from 3 to 6 samples of air were oolleoted from the ohamber for analysis, depending upon the length of the period of exposure. When exposure was repeated on tho following day, a corresponding number of samples was oolleoted on the aeoond day. Analyses were made for one or more of tho following materials! phosgene, hydroohlorlo add, free oreeols and oarbon monoxide. HONS 05599?  yirv - 13 - .CONFIDENTIAL When Aroolor 121(8 vaa to bo determined, the samples of sir were oollootod In an evaouated *:las# bulb 21.455 11tora m oepaolty. The Aroolor in the cample waa absorbed In 100 ml, of redletilled ethyl oloohol, the letter being released Into the bulb from on auxiliary bulb by opening an interposed stopoook. In the first few nnalyoon, It wee found nooeseary to l'roe the olcohollo solutions from carbon coot by filtration, but subsequently trio nood for ro doing was eliminated by the Inocrtlon of a plug of glass wool between the ohambor. and apperatua for collection. Suitable aliquot portion# of the eloohollo solution wore a,-.ployed in the ennlyaea, v.'hon phoBfOne was to be determined, the air withdrawn from the chamber was passed at the rate of 6,i) lltera per minute first through a column 1.5" x 0.5" of 60 to CO moah sino dust, next through a fritted . lose dlao in arsed In 100 ml. of a 4 per cent aqueous solution of sodium hydroxide contained in a cylindrical tower 13" in height and 2.25" In outside dlamoter, and lastly tlirouf-'h an l.-iplnper similarly im-orsed In a like volume of alkaline solution In a aeoond tower of the same dimensions, Famploa of air to be analysed for hydroehlorlo aold were oolleoted similarly exoopt for the omlSBion of the ooluron of alno dust (of. Jacobs, Analytical Chemistry of Industrial Poisons Haearda end Solvents, p. 305. 306, HONS 055998 - XU - .CONFIDENTIAL 1941). i S. tor on aliquot portion (lp ml. or lost) of the rolution had been nade sold to phonolphthalein with 10 per oont. nltrio aold (1 vol. of HMOj iJ.0. 1,4? diluted to 7 volumes with wator), and diluted to 24.0 :.l. In a volumetric flask, 3 drops of 0.1 f< ('liver nitrate wore c.:dod. i`iiO tratirmlealon of 11: lit of wave loiv.th 400 ryi throv/.h f.-iia turbid solution in a >0 rm. coll was >ensurod In a '.ookman spectrophotometer in comparison with at fchrou.-.h a i l'Ilnr cell contain.)rip a ohloriue-frce rolution prepared in a corresponding mannor. A ourve relatin'.' the tranawiaalon at 400 tyt to tb* concentration of sodium cVilorldo over the range of 20 to 320 nloro;ram per ?J> -1. Is ahown in /-l.fure 0. One i-illilrram of sodium chloride la oqulvalont to 0.6461 mg-,. of phosgene or to 0.6230 iv. <>f hydroohlorio acid. The oroaola were determined oolorlmetrloelly after coupling with dianotlaod eulfanilio acid by mothods for the meta isomer desoribod by kosnot and 'lanan (Anal. Chpm. 201941. 1748)# a''11* for the par* leomer by nohmidt (J, idol. Ohom. 3791211. 1949). Oanplee of air for the determination of either trleroeyl phoephate or free oroools (meta and para itomora) wero passed at t-he rote of 6.0 liter# per minute for 30 to 45 minute* through a frittod-clae# diso scrubber and lmplnger in eerie* a* in the oaee of phosgene. In this lnatanoe, eaeh tower HONS 055999 PONFIDENHAL 15 contained 100 ml, of a 1 pot* cent sodium hydroxide solu tion. In analysing for the froe oresole, 1 nl, of & 20 per oont sodium earbonate solution woo added to an aliquot of 6 ml, or loss oontalnod in a 10 ml, glaae-etopporod graduated oyllndor, Aftor tho solution hod boon diluted to 6 ml,, 2 ml. of dlaeotlited eulfnnjllo acid wore sdrtod, The 1 attar was prepared by add Inc 0,75 ml. of a 5 par oont solution of sodium nitrate to 0,75 ml, of a solution of rulfanlllo acid oontalnod In a 25 ml. volume trio flask previously oooled In on loo-bath. tho eulfanlllo add solution was preparod by dlenolvlng Ip,f> (> of tho acid in )j5 r.l. of concentrated bydrooblorlo acid end dilutln:' It to 500 -,1, with wotor. Alter the partially dlnxotleed solution had boon oooled for 5 minutes, an additional 3 ml, of oodium nltrot* solution were added and further oooled (or 15 .ulnutos, then diluted to 25 ml, i'he solu tion was preparod dally oral kept cold, Curvoe rolatiug the transmission of lip,lit of wave lo.v.the ip?;; ty< end 500 tyu to the concentration of -.ota crosol over the rcrr;o of 3 to !|9 mlorofrasie per 100 ml, ere shown In figure 9. rn making the neoossary measurements, the ooreperlson-oell oontslned a solution similarly prepared oxoept for the omission of orssol. Corresponding curves for pava-orosol over ths range of 10 to 120 nlorograwe per 100 ml. are shown In figure 10. By tho uae of the following HONS 056000 ..... fipNFIOCNT(Afc 16 ainultaneoue equation*, the amount of :aeta end perm oreeol oould bo oaloulatod. ( For the determination of mete oreeol (f)t p-orcsol 1| .31 il-oreaol * apparent p-oreol (4?5> iy<) pere*ol + 1.13 M-oresol apparent p-oreaol (500 iyt) 3.10 M-oroeol difference In apparent p-croeol at the Z wave length* (V) :i-oroeol * fllfforonoo In apparont p-creaol ...................T.10 or tho dotornlnntlcm of para oresol (Y)i :!-ororol + 1 r p-orosol apparent M-or*eol (425 vy*) 4.13 iororol 1__ _ p-oroaol apparent ii-oresol (500 pp) 1.13 0.653 p-oroeol * dlfferenoe In apparent ;<-oreaol at the 2 wave lengths (Y) p-ot'oeol dlfferenoe In apparent '^-oroeol 0.653 " fhe validity of thla method J'or the almultaneoue dotorilnation of the two lno.iore wee demonstrated by the following determination* on eoMple* containing both of thorn In known amount*. Analyale of a aiviple prepared to contain 12.30Y of m-oroeol and 9.96ZY of p-ore*ol yielded 13.90 end 9.95*. reepootlvoly. A rooond sample preparod to oontaln Zli.OQr of m-ore*ol and 9.96Y of the para laomer yieldod 25.90V and 10.7Y, reepootlvoly. l'ho rerultr do*lf.notod a* froe oreeola In Table 4 roproaont the aum of tho two leomora. From 60 to 70 per HONS 056001 - 17 - CONFIDENTIAL cent of tho total amount of fro* oreeola present in the ohamber, as the result of decomposition at 1050 F., mm found to oonelat or tho moto isomer, hut uhon tho torn* porature of tho furnooo wae 1250 F., only tho mo to Isomer wo* present. 'ilneo a sample of trloresyl phooplioto, analysed sf lor hydrolysis, oontslnod approximately 65 por oont of tho noto and 35 por oont of tho porn isomer, it uppoora likely that tho latter underwent decomposition to unldontlfled fragment* at tho higher teraporature. For tho deterra1notIon of undecomposed trloresyl phosphato proaont In tho oharaber, o P nl, aliquot portion of tho sample, oolleoted In tho manner Indloatod for tho detox'tnlnation of crenols, voa hydrolysed by refluxing It for an hour with 10 ml* of 45 per oont oquoouo o<)lum hydroxide! It wee thon aoldlftod with oonoontrated hydro chloric odd and distilled until 300 ml. bRd boon oolleotod. Tho usual orosol method was thon applied to tho distillate. Carbon monoxide was determined by a modification of tho mothod of Bland (Aust. J, Jtper. bid, and Med. hoi, 3j0i35 1940). The sample* were collected by poealne air at tho rate of 47 lltora per minute for 5 mlnutoo tlirou;.'h the abaorptlon train chown In Fleur# 4 by moan* of a cast notary Bloat and huotlon Pump located benoath the table and not shown In tho photograph. The flrat U-tube oontalnod glass beads and a 10 per oent aqueoua solution of HONS 056002  18 . CONFIDENTIAL load aootate, while the eeoond U-tubs, which contained tulfurlo eold (9.(it 1.04) and glass head*, was surmounted by a Ion,', narrow tube paoked from top to bottom with euooseelv* Isyore of silica eel (li-iprognated with a oobalt aalt to Indloate the presenoo of moisture). .>n/vnotilum porohlorate (Anhydi'ono), a eodluni hydrnto asbestos Absorbent (Ascarlts) and a second layer of a. noslum porohlorate. air Insulin from the top of thin tube passed through a tube containing Iodine pentoxlde heated to 115' C. iodine liberated by the reaction 5^0 --+ 5C0g * Ig vine oolloated in a bubblor containing 5 nl, of a 3 per oont aquoous solution of potassium Iodide, i'he rate of flow was honoured by an upright manometer as shown In r'lhnvo Ip. The oontonte of the bubbler vioro titrated with 0.001 U arsenlous acid, employing starch oa lndloetor, HjAsOj * Xg + '.!g0 --* !!jAsO^ + 2H1 One milliliter of 0.001 N arsenlous sold *0* 0.070025 mg. of 00. In calculating the results, sllowanos was mads for the previously determined traees of free iodine In the volume of the solution of potassium Iodide present In the bubbler. ;>soh of the following concentrations of the various oontsnlnants of the air of the oharaber. oppressed as HONS 0S6003 19 - CONFIDENTIAL p.p.m, by voluna In air at 64 F. and 745 ran. of roeroury, 1 equivalent to tha Oonoentratlon of 1 ng. per liter. goBBftWti Phosgene 1101 Xrloreeyl phosphate drorol Carbon monoxide Aroolor 1248 255.9 693.3 68.6 233.8 902.7 131.7 hxperlmontal Heaulf consisting of a oat, 2 guinea pica, 5 aloe, 2 rabblta and 3 rata, eurvlved whon exposed to air laden with 3.8 microprone of tydraul F-9 per lltor for 7 boura on eaoh of 5 oonsooutJ.vo day* (Table 1), All animal a of a group similarly oonetltuted, except for tha omission of ona rat, eurvlvod whan exposed to air containing 431 mlorograme of Aroolor 1248 for 7 houra on aaoh of 4 oonaaoutlra daya (Table 2). Lxoopt for ona rabbit (1790) that died from oauaaa unrelated to exposure 4 days after tha 6th expoeuro, those of a 3rd croup survived whon eubjeotod to tha inhalation of air boaring 61.5 miorograras of trloreayl phosphate for 7 houra on oooh of 8 daya ovor a period of HONS 056004 I f! -T' *a-v*r . - . ^CONFIDENTIAL" 20 10 day* (Table )). Change* In weight were umllj email (Table* 1 - 3), end no eigne of lntoxleetion were observed. Mloroeooplo examination of the lung* from animal* oxpoeed to pydraul F-9, and killed 10 or 13 day* after the 5th period of exposure, revealed the ooourrenoe of intoretlticl pneumonitis In the rat, mouse, guinea pig and rabbit. Die oat exhibited moderate number* of hlr- tlooytoe, containing dark-brown granular pigment, within respiratory bronchiole* and alveolar duote. The remaining vlsoera were normal. . Animals oxpoaod to the vapor of Aroolor 1240 also had Inter*tltlal pneunonlMa. the lunge of a oat whloh .hod boen subjeoted to exposure to the vapor of Aroolor 1?48 wera normal, fhara wae no evidence of Injury to the other vlsoera of the varloue speolea. The liver of a rabbit (1907), the only one examined mleroeooploally, exhibited severe fatty degeneration and fool of necrosis. Mloroeooplo examination of tissues from a rabbit (*'798) that died 4 days after the Oth exposure to trl- oresyl phosphate disoloeed the preeenoe of a severe parealtlo lnfoetatlon In tho liver. Another rabbit that was killed 3 days after the Oth period of oxpoeuro bad lnourred severe parasltlo Infestation of tho llvor, A guinea pig had aoute lobular pneumonia, unrelated to the experiment. In the lunge of a oat ware considerable MONS 056005 - 21 - jp P N F | D E N r (A t numbers of hlatioeytea, containing dark-brown granule*. The vleoara of the rot* and raloa wore normal. Tnoonol at 1250 or 1050 E.. rho duration of exposure, tho ohftnge in weight, and tha fata of Individual anlmala that wore eubjeoted to tha Initiation of tha produota firleing; from the oontaot of lydraul F-9 with lnoonal at 12J>0 and 1050 1'. are proaented in Table* *> and 6, roepeotivaly. Correapondlng data from animal* expoead to tho foe* from Aroolor121$ or trioroayl phoephat* are glvon, rocpeotively, in fdblaa 7 and 6 and in Table* 9 end 10. The data relevant to mortality among all group* have boon eummarleed in Table 11. 'lortallty. Caroful examination of tho data demon* ol.ro to certain .. onerel facta, It 1* apparent, in term* of ovor*all mortality, that i:uinoa plga and nloe war# ^ more ini;ooptiblo to oaoh of the throe material* than wort rata and rabbit* (Tablo 12). The *ame orltorion w* employod for oo*p*rlon of tho fog# that ware formed at 12^0 and lOiJO F. The fog generated from Aroolor 121$ at 12>0 F. wa mor# toxla than that formed at 1050 (Table 13). In tha aa*a of ^ pydraul K-9, tha toxloity of tha fog formed at tha higher temperature appeared to be elightly greater than that HONS 056006 -?:NF|>CNTJAL 22 generated at the low**1, The temperature of dooompoiitlon was of little Importance In determining the toxicity of the tots fowied from trloroeyl phoephate, slnoe that whloh resulted from the higher tomporature was apparently lightly toxlo than that formed at the lower toiler- atore. A comparison of the three material* under corresponding oondltlon* Indicate* that the foe* formed from Aroolor 1248 were more toxlo for guinea piga, rabbit* and nloe, but not for rat*, than were those formed from Pydreul : -9 or trloreyl phosphate (Table 12) Aroolor 1248 le also the most toxlo of the three material* If the *horte*t period of exposure that is lethal 1* taken a* the orl.orlon. Regardless of the temperature of doooi%>o*ltlon, all animal* survived whon subjected to the fog formed by fydraul f'-9 or trloroeyl pho*phate for 1> or 20 minutes, but exposure of like duration to the fog oroatod by the thermal decomposition of Aroolor 1248 often resulted fatally. In order te ascertain the effeet of different rates of dollvery of the liquid Into the Inoonel tube while the rate of flow of air through the tube was kept con stant, Aroolor 1248 and trloresyl phosphate were delivered at two different rates (Tables 4 and 11). The mortality associated with reduction of the rate of delivery was relatively low In many instances. M0NS 05600? C.ONFlDCNTIAk 23 flfine and Pathology of Intoxication. Although In those oxperlmenta the fog* rilled the olvamber to suoh n oxtent that It vaa lmpoealble to observe the anlmale tliroughout the period of oxposure, a l'ow signs of intox- loatlon oould be observed. The fogs were Immediately Irritating to tho wuooub i-ionbranes of tlio animals, tvs ovldonood by tbolr closure of tho eyes, twitching of ine nose, orylng, coughing and sneeslng. labored rooplratlon and Irritation of tho superficial tissues of tho oyos nppoarod later. Dyspnea waa observed after 20 minutes of exposure to Aroolor 1?46, but this did not appear until the animals bad boon exposed to tho othor retorlnlo for almost an hour, i.lienover oats wore exposed exoossive salivation was ovldont, and frequently long strands of thlok nuous drooled from the mouth. Maolt root oovered tho hair of animals exposed to fogs generated at tho hi; her temperature (1250 F.). Examination of animals that died during or shortly after oxpoaure to tho fogs of fydraul f-9 Aroolor 1?J|8 or trlorosyl phosphate revealed extensive pulmonary eongeetlon end. In oooaslonal animals, fool of pulmonary . homorrhogo, .Many olso had fool of black pigment that appeared to be oarbon distributed throughout the lung tissue. Mlorosoopleally, the loelons oonslsted of marked hyperemia of tho lung, oontnon to all exposed animals MONS 056008 - 24 *HTlA examined, and in many there hw lntra-alveolar hemorrhage nd edema. The oarbon pigment we* distributed ehlefly within the bronohi and, in eon* instance*, completely odbluded their lumen* iulmonsry emphyeewa vae prssont frequently. In 5 guinea pipe and 2 rabbit* tht died during oxpoeure thar# was, in addition, an aoute purulent bronohlti*, apparently a raaotlon to bronohial irritation. Lethally poieoned animal* exhibited paronohymatoue degen eration of the liver, kidnoy and brain with regularity, in addltioh'to^thd, pvCMw:y leaion*. the vleoera of animal* that died 7 to 10 day# aftor brief periods of exposure revealed pulmonary leal^na almllar to those already deioribed extepi that there ivae more pliagooytosl* << ' f'. of carbon partial**.* Anlrtal*. that survived were killed after intervale varying from 1 Week to 2 month#, there "were no significant lesions of vlaoera othsr than lung, two oats of the 5-exposed to the fog of iydraul i-9 wore examined miorosooploally. The vleoera of Cat A)8l were normal, while the lunge of Oat *374 were the site of pro fuse intraluminal bronohial deposits of black granular material and interstitial pneumonitis. The lung of Cat *371, whloh had boon eubjeoted to 2 periods of exposure to tho fog of trlcreayl phosphate, was ohareoterl*sid by almllar pulmonary changes. Oulnsa Pig A862, Jelled 22 day* after 2 period* of exposure to trloresjfl phosphate HONS 056009 -c Oyv /io 85 vaporised at 1250* F., was found to have numerous hlatlooytes loaded with oarbon pigment In the lungs, as well a* a severe Interstitial pneumonitis. Other epeoles of animals had a similar Interstitial pneumonitis. However, animals that had suffered the shortest periods of exposure, and animals killed 2(5 days or longer after exposure, exhibited normal vlsoara. The Oonoentratlons of Identiflad Pepompoaltlon Prod ucts Formed at the Higher and Lower Temperatures. Although only a port of the materials metsrsd Into ths Inoonel tube oould bs aooounted for In the air In the ohamber, some very tentative statements oonoernlng the composition of the air may be made. Although large amounte of soot were formed, no attempts wsre made to measure the amount of free oarbon or of oarbon dioxide. More unidentified materials v/ere present when the decomposition was effeoted at the' higher temperature, Irrespective of the nature of the liquid dropped Into the tube. In the case of Fydraul F-9, less hydroohlorlo aold and more free oresols were present when the temperature of oontaot was 1050* F. than when It was 12j50* F, (Table. 4). V/hen the temperature of oontaot with Pydraul was 1050* F., the oonoentratlons of hydroohlorlo aold and phosgsna, measured together In terms of hydroohlorlo aold, ranged from 0.35 to 0.67 p.p.m., but when the temperature HONS 056010 - 26 - 0/ /*> n was 1250 y. ths corresponding values ranged from 11 to 82 p.p.m. J'ree oresolo were more abundant at the lower temperature, being present to the extent of 11 or 12 p.p.m., whereas only 0.09 to 0,15 p.p.m. were found when the temperature was 1250* ?, In experiments with Aroolor 1243, nuoh more undeoom- poood material wee found present when the temperature of oontnot was 1050 V. than when It was 1250 K,, 121 to 286 p.p.m. being found In the former oases and only 10 to 16 p.p.m. In the latter. Only rarely was more than one p.p.m. of phosgene found regardless of the temperature, tho values reported as hydroohlorio aoid and phosgene representing predominantly the former. Loss hydroohlorio aoid was present at the lower temperature, there being 11 to 17 p.p.m. in this oase and 84 to 102 p.p.m, when the temperature was 1250 K. The values reported for oarbon monoxide are probably somewhat low beoause the rotameter employed for the oolleotion of the samples had not been oallbratod for use under the conditions of these experi ments. Nevertheless, It seems apparent that whon the lnoonel was kept at the higher temperature the amounts of oarbon monoxlds found tended to lnorease as the duration of the experiment lnoreaaedj muoh less variation was onoountered when the lnoonel was kept at 1050 T. HONS 056011 - 87 A'/r lb In the oase of trloresyl phosphate, more or the undeoompoeed materiel ana more free oreeole were present In the ohamber when the temperature of the Inoonel wee lower. The values reported (Table 4) for trloresyl phos phate, as determined by hydrolysis and the oolorlmetrlo determination of the resultant oresols, may be somewhat low, oinoe results several fold higher oould be obtained by measuring the ultraviolet abeorption at 64 eyM- of aloohollo solutions of spot samples oolleoted In an evac uated balloon. Determinations by this means may, however, have given high results, slnoe filtration failed to remove a slight smoky turbidity. HONS 056012 Co Nf? id N rUi 80 It 1* unlikely that any of the fatalltlos In thea experiment# oould be attributed solely to the preaerro* of phosgene, elnoe, ecoordlng to I'lury and Zornlk (Harmful usees, Gorlin, 1931* p. 888), raloe must be expoeod for several hours to the oonoentratlon of 1,85 p.p.n. If any are to bo killed, while only 70 par oent of a group of mice dlod when exposed for SO mlnuto* to air bearing SO p.p.ra. On numerous oocaslona the mlee In our experiment# dlod when exposed l'or but one hour to an atmoaphere that contained loaa than 1 p,p,n, of phosgene. Flury and ViOrnlk atata alao that rabbit* survived following oxposure for 50 nlnutoe to tha concentration of 50 p.p.m., but that 75 por oont of another group died whon expoeod for twice that period. Box and Culluitibina (Br. J. ftiarmaool, and thorn, t30, 1947) report tlrat 8 of 18 rats died after bolus exposed for 10 nlnutes to 0.83 ng, of phosgene per liter of air (57*5 p.p.m), although tha oonoentratlon of O.OGO mg. per liter (80.5 p.p.m.) vaa not lethal for rata when inhaled for 10 minute** In the oonoentratlon of 1,3 to 8,0 tug. por 1. (1174 to 1805 p.p.m.), oarbon monoxide caused no elgnlfloant slgna of Intoxication in mice expoaed for 6 hour* by Cherno and Liberman (Pannekol. 1, Tokelkol. Afit (Ho, 3)t88, HONS 056013 19471 0* A, 42,(2679 1949), nlthough SO to 40 per oont of group of mIm died * result of a Ilka period of exposure to th# concentration of & mg, of oarbon monoxide por 1. (5416 p.p.m,). Slnoo tho mice In our experiments (Uod under conditions whleh were nuoh le* sever* with respeot to oarbon monoxide, It la evident that thla com pound by ltaolf was not roaponalbla for their death. Comparable information with roapoot to other apoolaa of nnlmale la not readily available, although Moae, Jsokson and Selborlloh (Aroh, Ind. Hyg, and Toxlool. k)6S, 1951) hava reported that tho concentration of $,000 p,p,m, was required to kill a rat In 30 mlnutee, Rabbits and guinea pigs survived when subjooted to tha Inhalation of tha vapora of hydrogen ohlorlde In the approximate concentration of 250, 700 and 1,000 p.p,m., over the respective perloda of 7, 2 and 0.08 hours ('freon ot al,, Aroh, Ind, Hyg. and Toxlool, i?l6, 1950), In thoae same experiments, come of the guinea plge and rab bits died whon expoeod for 0,5 to 5.0 hours to oonoentra- tlona of hydroohlorlo sold ranging from 1,000 to 400 pp,m. Again, hydroohlorlo aold doe* not seem to be the eolo agent responsible for the deaths encountered in tha present experiments. HONS 056014 Data pertaining to the toxlolty of oreaola In air are not available} however, Deichmann, Kltemlllor and  30 {o. N >V, Witherup (/w. J, Clin, fatb. ^173* 1944) found that only after 20 perloda of expoaura, eeoh of 7 houra, to tha vapor of phenol In the air in tha oonoantratlon of 0,1 to 0,2 mg, par 1. did any raaplratory diatraaa or fatalitlaa develop among a group of 12 guinea plga liabbita and rata furvlved much longer# Slnoe it la likely that tha vapora of mate* and pora-oroaol ara comparable in toxlolty, it doaa not appear that tha doatha raportad haraln ora attributable to oroaol. HOUS 056015 mmMJM Experimental Hothode. In eooordanoe with the tech nique of Dralso, Woodard and Calvary (J fharm. Kxper. iharap. 02)377. 1944)# one of a aarlaa of graded dosage* of oaoh of tha undiluted material*, lydraul F-9. Aroolor 121)0 and trloraayl phoephata, waa maintained for 24 houra in contact with tha Intact akin of ona of a group of rab bits. In this method, tha hair waa ollppad from an area of MtIn 6 to 7 Inches wide and completely enolrollng the trunk of eaoh rabbit. In other groups, tha outermost layer of akin waa abraded by eoratohlng thin furrows 7.5> om, in length and a few millimeters apart with an hypo- demlo needle. ' In either ease, the ontlre trunk was covered with a oleovo of dental rubber dam whioh fitted tightly at the oophalad and oaudad edges of the dipped area and loosely over tho Intervening area. During the 24"hour period of oontaot tha rabbit waa kopt in atooka. At the end of the 21) houra, the material waa removed from tha akin of the animals by washing with water and a aulfoneted oil, *pH6". In addition to the three material* mentioned previ ously, a produot designed to fulfill tha eane purpose, HOMS 056016 I 32 - '*to Oergoyle 1XTK, wee lnvoetlceted. Gargoyl* DTK (Sooony) in nnld to bo o petroleum base hydraulic fluid oontelning minute amounts of n runt inhibitor ond on oxidation Inhibitor. Osrgoyle DTK he* tho speoiflo grerlty (API) of Jl.l, moxlntUM pour point of 20 F,, a minimum flash point of 395 F.| a flrn point of 497 F. Itn vlnooslty at 100 F, 130 F and 210 F. Is 225, 116 and 40.5 S.S.U., ronpnotlvoly. Experimental Iionulta. Tho partlnent data of tho oxporlmontn relating to Pydreul P-9, Aroolor 1248, trloronyl phosphate and Gargoyle DTK are given In Tables 111, 15, 16 and 17, respectively, Table 18 summarises the oseentlal information In the preoedlng tables. Two rabbits died following the appliestlon of 9.4 ml, of Pydraul P-9 por kg, of body weight upon the lntaot akin of eaoh, uhoreas only 1 of 3 rabbits died whan tho dosage waa 6.0 ml. per kg, fiiroe rabbits sur vived when oaoh was aubjootod to tha application of 3.6 ml, per kg. The dosage of 6.0 ml, per kg. was lsthal to 3 rabbits when applied upon their abraded skin (Table 14). . ' Two rabbits dlod following the application of 6,0 ml. of Aroolor 1248 per ltg, of body weight upon abraded ekln of eaoh, wheroae only 1 of 3 rabbits died when tha ^4 HONS 056017 CC 'V,,. 'b. 33 dosage was 9.If or 9.0 ml. per kg. The dosage of 9.4 ml. per kg. wee tolerated by 3 rabbit* when applied upon their lntaot skin (Table 15), The application of trloreeyl phosphate upon the lntaot skin resulted In the death of a rabbit given 9*1* ml. per kg. and in that of another given b.O ml. per kg. When the dose was reduoed to 2.5 ml. per kg., 1 of 3 rabbits died, whereas 3 rabbits, eaoh given the dosage of 1.6 ml. per kg,, survived (Table 16). Five rabbits with lntaot skin and 3 rabbits with abraded skin eurvived following the application of 9.4 ml. of Oargoyle DTK per kg. upon their ekin (Table 17). In no lnstenoe was the lntaot skin of the rabbits Injured by the application of these materials. Nothing remarkable ooourred when Oargoyle DTK was applied upon the abraded sklnj however, the abraded skin beoame very slightly erythematous after Pydraul F-9 or Aroolor 1248 had been applied. From the Kettering laboratory in the Department of. Preventive Medlolne and Industrial Health, College of Kedlolne, University of Cinolnnati, Clnolnnatl, Ohio. Experimental Work and Report byj Joseph F. Treon, Ph. D, Prank P. Cleveland, M. D, Prederlo E, Shaffer, M, 9. John Cappel, B. S. Thomas Oahogan . William Wagner Approved MONS 056018 Robert A. Kehos, M. D. Dlreotor . Date Maroh 11, 1953 o V. Table X Th Mortal tty, length. of .survival, end Phanaea In weight of Animle. Bxpea.od to the Vapor of rjr4mu3. Fr:9 in. Air (Gonoontretlon of 1`ydraul S-9i 0,00377 mft./l.) (Duration of ibcpoourei 5 * 7>0 iioura) Identification Number Chang* In height Sxproeeod aa i'oroontare of Initial violpht Initial During After Weight Period ol` Period of kg. Expoaura Exposure Kate Loncth of Survival Aftor '.xpoBure S'oforo the Anlmla Were Killed Paya oat A359 2.5'04 (.'Ulnoft H; A740 0.969 v.ulnoa A74-1 0.961 H ;lioe 3 ;loe ) Av. 0,0196 ) hobbit i CPli 2,038 iabblt FG25 net P539 1.963 0.246 Hat P540 fiat P541 0.209 0.252 -2.4 -1.4 -3.1 " -.4 -0.9 11.2 +3.3 -1.6 1.3 - 0.9 ' - *11.8 15.7 + 3.6 + 1.2 Survived 10 Survived 10 Survived 13 Survived 10 Survived . 13 isurvlvod 10 Survived 10 Survived 10 Survived 13 Survived 13 "OHS 056019 'p-'wvtyy*. *** "VWt<wW|IM| Mill ^ Mgyt^^ ^nam ^ SarTlyia, and P4*nM in Weight (Concentration of Aroolor 12481 0.0431 mg./l*) (Duration of Kxpoeurei 4 * 7.0 Hour*) ................... Change In Weight Kxpreesed ae Peroontag# of Initial Weight Identification Number Initial During After Weight Period of Period of <8. Kxpoeure lixpoeure fate Length ol Survival After i'jtpoaura Uefore ttw Animate were K11U Papa Get A3?8 4.300 Culneo Pig A7i>7 0.908 Guinea 21.8 A7i>0 0.939 4 ;tloe Av. 0,0178 babbit I<'906 .044 babbit K907 Nat K203 2.144 0.179 lint K284 0.170 - 2.8 4*8 - 4.8 +12.4 - 6.4 1.7 11.7 14.7 - 3.0 0 - 2.3 6.7 +13.1 +11.2 Survived Survived Survived 2 Survived 3 Survived Survived survlvod Survived Survived 6 6 6 6 7 6 7 6 7 HONS 056020 cv 'A'./>, MkLl tht Ror.taMt.yj- hngthj> gurvlvalj an4 Ohangaa l1UflJ.j5M.et Anl?)j-^AMA.Jig...tht-.y<>Bgg-af. mMMAJtaniHto in Air (Concentration of Trloreayl Phoaphatei 0.0615 mg./l., 4*2 p.p.m.) (Duration of Expoeurat 0 x 7.0 Hours) Change in Weight Expressed aa teroentage of Initial Weight Initial During After Identification Weight Period of Period of Humber Ice Kxpoaur# Exposure Fata length of survival After Apoaure bofore the Animal* War* Killed (K) or Mad (D) Day* Oat A353 3.226 Guinea fig a?33 O.809 Guinea l'lg A734 0.811 5 Mloe Av* 0.0164 Hobbit 1797 1.779 Hobbit *790 1.841 Hat 0536 0.130 Pat 0537 0.140 Rat 0538 0.129 + 5.6 + 1.6 - o.5 *13.4 + 4.6 + 6.5 *14*6 7.1 +20.7 0 + 4.0 . 0 m * 6.2 *25.7 * Survived 3 K Survived 3 K Survived 10 K 2 Survived 3 K 3 Survived . 10 K Survived Survived 3K 4 D (1) Survived 3 K Survived 10 K Survived 10 X (1) Death due to paraaltlo infestation of llvor unralatad to exposure. HONS 056021 Table 4 C Concentrations of Various Decomposition Products In Air of Chamber In the Several Bxpeid merits Material is Contact With Inconel Variation in Temperature of Inconel T. Experiment Number Material Added ml./min. Expressed as mg./l. on Upper Line and p.p.m. on Lower Line Aroclor Phosgene HC1 (uncor rected for Phosgene Trlcresyl Phosphate Free Cresola .' Carbon Monoxide Pydraul 1240 - 1256 1 0.143 - 0.032S 22.7 - O.OOO65 0.15 Pydraul 1245 - 1260 2 0.133 - Pydraul 1240 - 1250 3 0.167 - Pydraal 1245 4 0.245 - Pydranl 124-5 5 0.220 - - 0.01S0 12.5 - 0.0164 11.4 - 0.0155 10.7 -- - 0.00052 0.12 _i -I 33 - 0.0003S _ j 0.09 -- 3- --- ~| Pydraul 1040 - 1055 6 0.132 - - 0.0005 - 0.0459 * ~ -I 0.35 10.7 1 i MONS 056022 Table h (Page 2) lOt ht Material In Contact 91th Iaoonel Variation In Temperature of Inconel r. Experiment Humber Material Aided ml./min. Expressed as Eg./l . on Upper 1ins and p.p .m. on 101ser line . Aroclor Phosgene J5C1 (uncor rected for Phosgene) Trieresyl Phosphate ?ree Cresols Carbon Monoxide Pydraul 1047 - 1052 7 0.125 - Pydraul 1049 $ 0.113 - - 0.CC12 O.83 - 0.0435 11-3 T; i 0.00096 - 0.0437 i 0.67 11.4 -^ Pydraul 1049 9 0.14$ - - - 'i - 0.0513 12.0 - i? \ Pydraul 1047 10 0.100 -- Aroclor 1247 - 1256 l 0.113 0.124 0.00203 0.121 16.3 0.52 33-9 - - 0.24 1 216.7 1 Aroelor 1247 - 1252 2 0.144 0.111 o.oi36 0.147 - - . 0.03663 14.6 4.3 101.9 33-0 f 4 Aroclor 1249 - 1252 3 0.125 0.0773 0.0012 - 10.2 0-31 - - 0.0016^ . 1-* i - 1 "3 MONS 056023 Table h (Fage 3) Co Nf id r'A, Material in Contact With Inconel Variation in Temperature or Inconel o p. iiperirjent Xurjber Mp iorial Added al./rain. .741reased aa r-<,./l. on Upper Lice and p.p.ru on Lower List Aroclor phosgene HC1 (unearrected for Fhoagene) Tricrespl phosphate Free Oreads Carbdk aoonidi Aroclor 1252 4 0.130 0.102 - 13.4 - - - 0.0007! 0-7- Aroclor 1224-9 5 0.069 0.0573 0.0109 0.0143 - 7.5 2.8 9.9 Aroclor 1252 6 0.067 0.0387 0.0015 - 5.1 0.4 - Aroclor 104.9 - 1053 8 0.121 2.17 0.00035 0.0224 - 285.8. 0.09 15.5 - 0.025a* 22.7 -1 - 0.038^ 34.8 ; - 0.0260.* 23.5J Aroclor 1049 - 1053 7 0.122 1.80 0.00071 0.0154 - 237.1 0.18 10.7 - 0.030a; 27.8 ; Aroclor 1C49 - 1051 Aroclor 1049 9 10 0.121 0.120 1.37 180.4 1.24 163-3 O.0CO37 0.09, - 0.0253 17.5 0.0190 13.2 -. '- 0.0146? 13.2 ' 0.0220 19.9 i HONS 056024 Table 4 (rage 4) Co N FlD r'At-S Material In Contact With Inconel Variation In Ter^erature of Inconel r. Sxperlnent Suribar Manorial A-ded nl./kin. iixpressod as :-:g./l. or. ,u>e.- line and p.p.a. on Loner Ull Aroclor Phosgene SCI (uneorractod for irlcreeyl phosgene ) Phosphate ri*ee Cresols Carbea* SODOXlSi Arcelor 1051 11 0.120 0.918 - 120.9 - -- 0.0231 19.0 Aroclor 1049 - 1051 22 0.058 1.60 0.00052 0.0171 - - 0.0255 210.7 0.13 11.9 23-2 Aroclor 1050 - 1052 TricreaTl 1240 - 1258 Thoaphate 13 15 0.068 012& 1.31 172.5 - - 0.0107 7.4 - - 0.0049 l.i 0.012^ 11.6 ; i "t S70950 SNOW 00 1 Xrieresj-1 1249 - 1252 2 0.122 Phosphate - 0.00266 O.Q2M 0.6 26Jt i TJrteresyl 124? - 1253 1 0.121 - - - Fhoaphate Trier*yl 1257 Phosphate 14 0.233 - - - Triereeyl 1249 Phosphate I 6 0.210 - 0.0088 0.00158 0.0345 6.0 0.4 3l.l ; - 0.00676 1.6 - -. . 4 .fable h (Po^e 5) Material In Contact Vith Inconel Triereeyl Phosphate Variation In i'orperature of Inconel y. 1245 Experiment Straber 8 i'c.terlal Added nl./stxn. 0.071 iixprecsod as : . ,/i. on Upper iJ.no and p.p.n. on lover Um Aroclor - Phosgene - SCI (uncor rected for phosgene} - Tricresyl Phosphate .- i7 Free Certce - Cresole acn-ori-asi 0.0011 0.3 '1 Tricresyl Phosphate 1258 frlcresyl Phosphate 1045 - 1057 Trlcreayl Pboephate 1049 - io5l Tricresyl Phosphate 1025 Tricresyl Phosphate 1053 Triereeyl Phosphate Tricresyl Phosphate Trloreeyl Phosphate io5i 104.9 - 1051 1051 7 14 13 3 10 11 9 12 0-C72 C.130 0.125 0.120 0.175 0.195 0.088 0 *Co2 -- - - ---- ---- - - - ", * - - 0.011 2.6 3 - - 0.098 < 23 - o.55o 0.0565 0.025* 37.8 13 22.6 j i - - - * ^ 1f - -: !i 0.35 81 1 -' - 1 970950 SNOW .CCf ,N' 7" M i The Xor iallty. Un. th cf 'urrivcl. a'wl Clicrvcs In `. cl, ht cf --xucged to Aerosols > or- oi by < ydraul r-9 InUo &ii Xncur^l .Tube . lieate-j to f- .`cnfrsgsvjre of 1250 - (677 ^ ) 2xj>or- Duration of iaent ^exposure Identif ication Sunber Hours Nunber Initial Weight kft. Change in Weight expressed as Percentage of initial weight During Period of exposure Ai'tor Period or lix^oi-ure Fate as Length of ourriTal icted at of Anhsale That 2nd of . Died (D) or Were best Killed (x) Period of After Lest ihpoaure Period of Exposure ` 1 6.0 + 6.25 Cat A37b . 2.9i;5 -lo .0 6.0 Guinea Pig A759 0.620 - 1.2 6.0 6.25 Guinea Pig A760 1.020 -11.1 6.25 ,, 3 KiCe 6.0 6.25 2 Mica ) ) Av.i'.CS; 6.0 * 6.25 Babbit F93? 2.620 - 6.S 6.0 * 6.25 Rabbit B533 2.550 - 4.2 6.0 Hat H301 0.207 - 1.0 6.0 Hat 302 0.20S - 1UP +11.2 + l.s +10.0 + i.5 hurvived hied -urvived Died .'.-urrived iurvired Died /led 7 days X 7 days X 7 days K 7 days K ' 2 6.75 6.75 6.75 6.75 6.75 6.75 6.75 6.75 6.75 Cat A379 3.170 Guinea Pig A761 0.6-50 Guinea Pig A762 0^2i5 .House AU35 0.025 3 Mica /.v.0.020 Rabbit ?538 2.267 Rabbit P935 2.270 Rat H303 0.160 R*t C.205 - 9.9 - 1.8 0 - .0 0 0 - 2.2 - 4.9 3*3 -- - +16*0 - +11.2 + t eil iurvlved ,'ied i)iod burvived GlOd .rvived OurviTed 6;ld Died 2 saonths X -- - 15 days K -- 6 days X C days X - " 709S0 SNOWI a Xaolo 5 (rav 2) Exper Duration of iment Exposure IdectIflentIon Rumber Hours Suaber Initial weight fee* Change in Weight Expressed as Percentage of Initial weight During Period of Exposure After Period of Exposure Fate as Soted at End of Lost Period of Exposure Length of GurriTal of At1 wale That Died (D> or Were Killed (K) After Last Period of Exposure 3 3.1 3.1 3.1 3.1 3.1 3.1 3.1 3.1 3.1 Cat A370 Guinea Fix; A7&3 Guinea ?l A764 3 "Ice 2 Mice Rabbit F93& Rabbit F937 Rat 22305 Rat E306 3.0G0 0Jl65 0.420 Av.0.015 At.0.0175 2.170 2.470 0.200 0.200 -7.5 - 2.7 - 7-1 . . C -- - 3*5 -1.0 +10.0 + 9.2 - + 6*iL - - SurTlred Died Gurrired Died c-urriTed Gurrired SurrlTed Died Died 7 aya 7 days -- 15 days 7 days 2 day* " K ID K' K 9 . 4 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0-9 Cat A3S1 Guinea Rif. A765 Guinea i-I : A766 4 Mice 1 Vnuse Rabbit 93-3 Labbit F939 Bat =307 Eat E3oa 2.325 0.440 0.410 At.0.017 c.ci5 1.998 2.030 0.205 0.188 -1.9 -1.6 0 0 . 0 0 - 6.8 -10.6 - + 5-9 +53*3 27.1 +27.6 25*ii 32.4 survived Died curvired 4>ied GurriTed .'urrired GUTTiTOd :urvlred : urTlTed 9 days -- 15 days -- 14 days 15 days 15 days 14 days 15 days K s K K K K K 5 0.25 0.25 0.25 0.25 0.25 0.25 0.25 Guinea ilr A7&7 C-uinea Fig A768 5 Mice Rabbit 794-0 Rabbit' F941 Rat S309 Rat i.310 0.450 0.460 At.0.0156 2.115 2.050 0.187 0.188 - 2.2 - 4-0 0 * ^*4 0 -14-4 - 6.5 + t.4 +10.7 + 6.3 +37.9 +36.9 +30.3 v-arrived . urrired : urrired urvlTod GurriTed Gurrlrod iurrlTSd 24 days K 24 days K 26 days K 2c days K 26 days K 26 days K 28 days K - HONS 056028 Pablo 6 Pc :<::;:NriAc The .`ortalltr. Lcrx th cf : nrvlYel. rr.d Cbsn- os Ir. -.el; ht of AnJ-EsIa exposed to Aerosols formed by ./rop.ln/ i draul f-y Into a~ Incor.ol Pubs Heated to a ioreperatura of 1050 P. (566 C.) Exper duration of iment ixposure Identification Susibar Hours Humber Initial Weight Rg. Change in '.eight Expressed as Percentage of Initial .eight During period of exposure Al ter period of exposure Fate as Noted at Lest rorlod of j&cposur# Length of surrirul of Aninale Shat Died (S) or Were Killed (X) .. After Last . Period of Exposure 6 6.5 * 6.8 6.5 6.5 6.5 6.8 6.5 6.8 6.5 b#8 6.5 + 6*8 6.5 6.8 6.5 + 6.8 6.5 6.8 7. 6.8 6.8 6.8 6.8 6.8 6.8 6.8 6.8 6.8 6.8 Cat A376 Guinea pig A769 Guinea Pi~ A770 1 House ) 1 House } 3 Hice > Rabbit F942 Rabbit F943 Sat E311 Sat K312 3.065 OJtSO 0.406 at.0.0184 2.190 2.140 0.235 0.208 . - 7.3 - 5.3 - 7.1 - -- -14-4 --2Geii - 4.3' Guinea Pig A771 Guinea Pig A772 3 Mice 1 Houm ) ) 1 House ) Rabbit F944 Rabbit P'9115 Rabbit F946 Rat 326 Rat 327 0.4.90 0.465 AT.0.017 2.055 2.140 2.410 0.230 0.280 - 5.9 - 1.3 a. - 5.1 0 -13.0 - 8.6 - i.2 - -22.6 33.2 + 6.4 +23.5 -12.1 24.6 -28.6 Surrlred Surrlred Died Died Surrlred surrlred Surrlred Surrlred Surrlred Surrlred Died Died Died Surrlred Surrlred Surrlred Surrlred Surrlred Surrlred Surrlred 3 months X 16 hours D 3 days 7 days 17 days 4 dsys 3 days 24 days _ S X S 9 D X 3 days D 5 days , 9 24 days X 26 days X 6 days 9 29 days X , 1 "8"Ul HONS 056029 .'able 6 (i'a: e 2) CC 3 2.VrrA. "i i Sxpop- Duration of inent Exposure Identification 5safer Hours Kuaber Initial Weight kg. Chssve in -.eight Expressed as Percentage of Initial weight During Period cf Exposure After Period of Exposure Fftue u l>oved at End of Last period of exposure Lcn-th of Survival cf Anisala that > Died (D) oar *" Killed {) After last < Period of ibqposur# e 3.4 3.4 3-4 3-4 3.4 3-4 3-4 3.4 3.4 3-4 9 0.9 0.9 0.9 0.9 * 0.9 g 0.9 0.9 0.9 S -9 O' 10 S 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 Guinea fie A773 Guinea Fig A774 3 Kies ) 1 .=4ouse } X House } Rabbit F953 Rabbit 2954 Rabbit 2955 Rat 328 Rat 329 0 ii'OO Av.0.023 2.000 2.250 1.940 . 0.230 c.230 Guinea fig A775 Guinea fig A776 2 Kice ) 3 Jtte ) Rabbit 5956 Rabbit P957 Rabbit 2954 Rat 330 Rat 331 0-430 0.405 Av.0.0162 1.934 2.1eo 1.670 0.260 0.230 Guinea Pig A777 Guinea Fl A778 5 Mice Rabbit F9S9 Rabbit 960 Rabbit F961 Rat 332 Rat 333 0.845 0.695 AT .0.0122 2.040 2.095 1.735 0.200 0.235 - 3.1 - 3-4 ~ _ -26.2 -23.1 -11.6 - 7.4 -12.6 - 1.6 - 3.2 0 - 0.7 -17.3 0 0 - 7.2 - 6.2 0 - 0.7 0 O - 7.5 -10.6 - +5o #0 +49-4 35.1 +2li.7 35.7 -36.2 - +27.5 +28.5 36 a6 +30.8 +34.3 +11.7 + 6.0 35.5 +43.5 +29.9 +57.5 42.1 Died Died Died Survived Survived Survived Survived Survived Survived Survived Survived Surrired Died survived Survived Survived Survived Survived Survived Survived Survived Survived survived Survived Survived Survived Survived _' -. --. 6 days D 6 days X 1.6 aonths X 18 days 28 days 27 days X 27 day* - 19 days D 1 day D -- 4 days X 2o d&j* 28 days 1.6 aontbs K 27 days X 27 days X 1.7 nanth* X 1*7 npAthft K 34 days X 28 days X 28 dsys X 28 days X 1 29 days X 29 days X 'able 7 :<~!0 = ht,ac The '-TortalltY. hearth of Survival. end Chan os to Weight of Aniaala paposod to Aerosols I'or-.ed by --roa-. Aroclor 1246 Into an Inconel Tube Heated to a Vetterstune of 1250 r~. (677 C.) . Srperincnt Suaber 1 2 Duration or iiXpOS'UPO Hours 7-c 7.0 7.0 7.0 7.0 7.0 7.0 7.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3-0 Identification Number Guinea i'l;: A7?9 Guinea Pig A7C0 5 Mice Rabbit F962 Rabbit >963 Rabbit P964 Pat ^334 Sat b.335 Guinea Pi- A762 Guinea Pig A7S3 5 Sic Rabbit iv;CG Rabbit F9&1 Babbit F?c2 Fat 6336 Rat a337 Initial Weight 5q> -.475 Av.0.0154 2.105 2.205 1.960 0.220 0.250 0.430 o.kito Av.0.0152 1.750 1.800 1.865 0.26? 0.240 Change in Weight impressed as percentage of Initial Weight inuring period of iuiposure After Period of iu-porure - 1.2 - 1.1 * - 1.7 - 1.4 0. - 6.6 - 6.0 _ * _ - k*> - 6.6 -19.1 - 9.2 -- - 1.9 -- 4* -29.S 7.4 - " Rate aa Doted at Dnd of Last period of ixposore Died (1) Pied (1) 5 Died Died (2} Died (2) Died U) Died Died survived Died (3) 5 i>ldd vuryired iurrlTad j;lod Died Died .' Length of Surriral ; of Aniaala 12mt Died (D) or -1 Killed <K} .. j After last , : ; Period of Eqweige i i -i _ - 9 days S - 27 days D 6 d--ays ' S ' j i '! 3 p1ij * ! j i \ -ii j s 14 HONS 0 5 6 0 3 1 xble 7 e 2) C. At Experiaent Kisabcr Curaticn of Exposure lours Identification Ltsber ini old teiihit 3 1.0 -uinea k i; A7rl -.113 1.0 1.0 Guinea ri A?c5 1 :-5ouso ) v.3.0 1.0 1 rouse } av.C.C'16 1.0 1 .'ous ) 1.0 1 -'out ) 1.0 HaSMt JvC3 1.7:5 1.0 Hafcbit F9C1 1.785 1.0 1.0 Rabbit r'955 Sat .>336 1.550 02*4.3 1.0 Fat e339 ..265 C'uui e in .Lcpi-essed as rorciu.tt e c-f Initial . ei; lit i eri'.xi oi* ^;.c*&ure Af tor i-cried ot -Af-crure . ate as Acted at ;uil of East . eried of -ueposur - 4.1 -- - - - - 0 0 V - 7*ii -10.6 -36-2 - - - +28.7 +27.3 +-5.0 +36.3 +30.9 -urvired urvivod .urvived : urvired 1urvired survived ' urvired .urvired 0urvived :urvivod :urvired Len_.tLi of Survival of AniraCLs fbiat Died (o) or Kara allied U) After Last forled cf Exposure 7 hours D - t days 0 . 5 minutes 3 1 day 6 days c days 23 days D o 0 : : ! 23 days ? 23 days 21 days s 21 days ! 4 0.33 Ouinet Pip A7C6 0.160 - C.7 :. urvivod 1 day 3 : 0.33 Guinea Piu; A717 - -->0.0 urvired V days C : 0.33 5 Mice AV.0.C162 -22.0 - > urvived 1 day D ' 0.33 Ii&bblb rSrC5 2.C7S - 1.1 + ab `urvivod 6 days s. 0.33 Rebblt Fy66 1.361 - -13.2 ; urvired 10 days o : 0.33 Rabbit F967 1.960 - 1.0 -Survived 6 days K 0-33 Rat 3^0 0.220 0 + t..S .survived 13 cays' 0.33 Fat Z3H 0.225 0 ;?</ ] -urvired 13 days HONS 056032 i fable ? (fage 3 ) O riOi.NTIAL exper iment Suonber i>urcvicn of i^xpcrure Hours Identification Kunber 5 3.0 Guinea ?lg f.7i& 3.0 Guinea fie A769 3.0 ' sice ) 3.0 i +ouse ) 3.0 Rabbit 5966 3.0 Rabbit P969 3.0 Rabbit 5970 3.0 Rat (31^2 3-0 Eat 2343 Initial ..eiiht k. 0 eill^G 0.415 2.160 1.6-/5 1 _f:70 0.260 0.215 Chan; e in rfeinht expressed as i'erc outage of Initial height During ireriod of ixxpcsure After icrioi of ^xvosure Rate as Goted at ind of Last terlod of Lxpoeurs -25.5 - 7-1 - 3-9 - 1.1 - *4. *6 - 4-7 -62.0 -63.3 -- -23*? -13.6 +19.7 8.1 + 6.G -- Gurvivod Survived Died : urvived Survived survived Survived furvired -;xdd Length of Survival of Animals Ttsat' Died (D) or Ker* Silled () . After Last : 9 days D 10 day* O 6 days 3 22 days 22 days 7 days 12 days , . . 6 1.0 1.0 1.0 Guinea Els A790 Guinea ii A791 5 Mice 0.605 c J*S i\7 C>QliU - 5.5 - 9-5 0 . -11.2 + 7.5 +65.9 survived Survived 5 Survived 1.6 months 1 month 2u days 1.0 Rabbit 5971 1-7V5 V +26.0 Survived 22 days 1.0 1.0 Rabbit 5972 Rabbit 5973 1.950 1.500 c 0 +3>.h +17.3 Survived Survived 1.4 month* 22 days 1.0 Sat 2344 0.330 v + 3.6 Survived 23 days K 1.0 Sat h.345 0.2vp 0 22.6 . urvived 23 days X (1) 2.25 hours Trot the start of exposure. (2) 6 hours fr^r the start cf exposure. (3) 1.5 hours fro- the start oi c vo.'ur. hons 056033 I -able o he Mortality, Len. oh -urvival, and Chen s Jr finals iasoaed to Aerosols joraed by Prom In,; Aroclor 12h& Into an Ir.conel fabe .lessee to -or?,erasure of 1CS0C &UP .) Experinent Hteber Duration of .sjcporura iovrs Idontificstion 'limber 8 ?.C Guinea tic- A 79a. 7.0 Culzzdfi i*it; AVvS 7-0 ii Mice ) 7.C 7.0 1 .'louse } 7.0 +7.0 Kebblt J977 7.C 7.0 Rabbit F-/7C 7.0 Rabbit F979 7.0 Rat -361 7.0 + 7.0 Eat 362 Initial Dei. ht *> Change in .ei; it hxj-rossod ss ierconta^e of Initial height i iroriod or AT tor i'viPlod 0" .-xposure i'a t as "oted. at Last roriod or exposure Length of furriTal of Aniicals That Die! (D) or Wore Silled (K) After Last . - ieriod of Kxpoaure 0-433 C* 2.060 2.200 2.175 0.260 0.295 - 2.3 - 2.2 - - 9.2 -1C.0 - 9.2 - 7.7 -, - _ . -32.6 Died Died 4 Died hurrived Died Died Died i>lad iurrived 12 hours S> _ - 5 days D . - 7 7.0 Guinea ?i< A792 c.li50 0 Died 7-0 Guinea Ei" A793 0.^20 - 1.2 - Died - 7.0 2 nice ) .e 2 Died 7-0 7.0 1 Mouse 2 Mlco } at.0.019 } - - iurvieed 5 days D Survived 2S days. S 7.0 Rabbit Fy7& 1.965 - 6-5 - Surrired 5 days D 7.0 7.0 Rabbit FV75 Rabbit F976 1.665 2.005 - 9*^ - c.l 44.2 +19-4 lurrired Surrired 1.3 aoQtbe K 1.3 isnwths X 7.0 Rat x.359 0.305 - 2.6 - Died 7.0 Rat 360 0.310 - 3-2 Died :! HONS 0 5 6 0 3 * ibcpcr- Duration cf iisant Ljcpoauro Identlfication Somber Hoops Hunber Vabie 8 (Pave 2) Initial Weight kg. Charge in 'Weight ^jqgpessed as Percentage of Initial Weight During period of Exposure After Period of cjcporure Coti obkT!Al - - , k "i F*te u .Noted at 2nd of Last Period of wtsposure Lou th of Surrl-ral cf Animals That / Died (D) ear Wqt* ' Killed (K) After Last .. Period of Stpoaurs 9 3*5 Guinea Pig i-7'/6 0.390 3.5 Guinea Pi0 A797 0.415 - 3-5 3 Mice ) - Died Died - 3 Died ^ 4_ -- -- . :" '* 3-5 1 House ) AT.0.019 -- - PurriT-ed 10 day* B . 3-5 1 isouse ) a. - Serrived 20 days K 3.5 habbit F9S6 2.320 -13.6 15*6 Surrlred X rtaonth S i 3.5 rVf27 2.250 - - 4-3 surviTed 5 days B 3.5 i-abblt PyOS 2.355 v *6 - Died 3.5 Hat H363 0.290 - 3.4 +23-1 I urrired 1.1 faiths X ` 3.5 Hat 364 0.240 -10 .0 +26.2 GurriTod 1.1 months S 10 1.0 Guinea Pig A798 0*455 - 2.2 Died - .-S 1.0 Guinea Pig A799 0.430 - 3.5 SurriTed 2.5 hoars B 1.0 5 Hlce Av.0.0190 - 2.2 +37.9 Surrired 20 days K j 1.0 Rabbit F9S9 2.040 -V - 6.9 Surrired 6 days D y 1.0 Rabbit 1-991 2.250 - 3.5 +17.3 Surrired 1 neeth K . 1.0 Rat i-365 0.315 - 4-8 +24.S SurriTed 1.1 nontbs X l.C Sat H366 0.305 - 4.9 +I6.4 Surrlred 14 days K ; HCiNS 0 5 6 0 3 5 11 0.33 Guinea ri-' ACOO 0.410 -45.1 G-urrlred 1C days B 0.33 Guinea Pi.- AS02 0.455 - 1.5 Died -. j 0.33 5 iiee .AVsQsOoO -26.6 GurriTed 19 dayd K 1 c.33 Rabbit PyV4 2.095 -11.2 +24.3 PurriTod 1 nenth K 0.33 Rabbit ?99> 2.120 - 3.2 --21*4 Gurrired 1.3 nmths X 3 0.33 Rabbit F996 2.1'/0 -26.1 urrlTed 12 days 9 ' . 0.33 Bat i.367 0.260 o- +29-6 urrired 1.1 souths X J 0.33 Hat 366 0.255 0 35.7 r-ureiTed i.A ******* * i if.*-. Table 3 (Page 3) Confidential 4... ld Exper Duration of iment Exposure Identification Stssber Sours Kunber Initial Weight kg. Change In Weight Expressed as Percentage of Initial Weight Ourlasg Period of Exposure After Period of Exposure Fate as Soted at End of Last Period of Exposure length of Survival, of Anlnale That Died (D) or Vara" Killed is.) After East Period of Sxpeaura 12 3.5 Guinea Pig Ac-02 0.460 - 3.3 3.5 Guinea Pig AO03 0450 - 2.2 -- Died Died - 3.5 5 iiiCO At.0.017 0 +22.4 Survived 16 days X'' 3.5 Babbit P997 2.210 - 7.2 +20.5 Survived 29 days X . 3.5 Babbit F993 3.5 Rabbit F999 2.060 2.090 - 5.5 +11.3 - 5.7 Survived Surrired 29 days X 5 dava D 3.5 Rat 369 0.255 0 10.9 Survived 1 sooth X 3.5 Sat 2370 0.23S O 26.7 Survived 1 sooth X 13 1.0 Guinea Pig aGC4 042S - 2.4 1.0 1.0 1.0 Guinea Pig AS05 3 Klee ) n 0. .405 1 House ) - 1.0 0 Survived Survived Survived Survived 3 houra 2> 3 boure D 16 d*y X 7 days D 1.0 Rabbit 02 2.340 -IS. 6 +40.6 Survived 37 daya X 1.0 Rabbit G3 2.410 - 6.6 24.8 Survived 29 daya X 1.0 Babbit <4 2.345 - 3.2 124. Survived 29 daya X 1.0 Rat 2371 c.230 - 6.5 9.6 Survived 32 daya X 1.0 Rat 2372 0.215 - 2.3 74 Survived 32 daya X HONS 056036 Table 9 n P / 0 ; NHAj The .iortalltT. Lon." ch of tyrrival. and Chan-cs In -el- lit cf Anigals exposed to Aerosols ferried by Drop'-Iry .rlcrecTl Fhoagiiato Isto er- Inconel Tube Heated to a iO;-?oorasyro of 1290 f (677 0.) Kxpor- Duration of insnt Exposure Sumbar Hours Identification iiaaoer Initial 'aieieht kg. Cuanre in sieiidit Expressed as Percentage of Initial .'eight during cried of Exposure After Period of Exposure Fa'^e r.-oiod at cf b&Sv >dried of Ajqxjs-ur# Length of Survival of Ariaala Shat Died (D) orWer* Killed (K) After Last ' Period of Exposure 15 2 x 7.0 Cat A371 3.611 - 6.5 2 x 7.0 Guinea Fir a562 - 6.0 7.0 Guinea Fig A563 Ci;3& - 2.3 2 x 7-0 5 Klee Av.0.0156 2 x 7.0 Babbit G17S 2.963 - 2 x 7.0 Babbit Gl?9 2.563 0 2 x 7.0 Babbit G180 2.791 - 6.9 7.0 Rat S679 0.14-06 - 2.2 7.0 Rat Sij.80 o^So * X.2 +11.9 -- +12.9 --156 +19.6 -- * Survived survived Died 5 Died -Survived Survived Survived Died Died . 7 days S 22 days A -- 17 days K 16 days D 16 days K -- 2 7.0 Guinea fig a8C8 c.6io - 6.9 -35.0 Survived 30 days D 7.0 Guinea Fig ASC9 c^i.95 - IJj. uiod -- 7.0 5 Mica Av.0.017 - 5.0 - p Died -- 7.0 Rabbit u8 1-965 - 6.2 Survived 27 days K 7.0 Rabbit C-9 2.010 - 2.7 +22.1 survived 27 days K 7.0 Rabbit C-1C 2.075 0 +33-5 GurviTod 27 days A 7.0 Sat H375 o.lv5 -16.9 - Died - 7.0 Rat H376 0.225 -12.0 " Died ' HONS 056037 Sxperirsent Staaber Duration oT Exposure Houses Identification Sunber Initl&X ..eight k. Change in weight .caressed as Percentage of Initial Weight ?vricd or .^xpocuro After Period of Exposure iate as Jotad at End cf Last period of Exposure Length of Survival of Anisala fhat Lied (0) or Were Killed UQ After Last . Period of Exposure 1 3*5 Guinea fig >,806 0.500 - 2.6 Died 3-5 Guinea fig AGO? C *4^Q - - Died 3.5 2 Mice } - 2 Died -- 3-5 1 Miouao ) Av.0.017 -10.0 - survived 22 daya C . 3.5 2 .lice ) +33-3 Survived 34 day* X 3.5 Rabbit C'5 2-270 0 4'jLii.8 Survived 28 days K ' 3.5 Aab'clt G6 2.110 0 +37.4 Survived 28 days X ` 3-5 Rabbit G? 2.140 - 0.5 +20.7 Gurvivod 28 day* X 3.5 Rat 373 0.220 0 + 2.3 Survived 34 days K 3.5 Rat 374 0.215 0 + 2.3 Survived 34 daya X HONS 056038 u. 1.0 Guinea PL- A22 0.445 - 1.6 1.0 Cuir.ea fig A-S23 c.430 - 5.3 1.0 5 Mice Av.0.0150 - 1.0 Rabbit 022 2.095 - 2.0 1.0 Rabbit C-23 1-995 2.3 1.0 Rabbit C-24 2.110 - 5.9 . 1.0 1.0 Rat i.399 Rat 400 0.215 0.210 - 6.0 - 3.8 6 0.33 Guinea fir- A 842 0.905 - 1.1 0.33 Guinea fl- AG43 C.8C0 0 0.33 5 Klee Av.0.022 0 0.33 Rabbit 0114 2.450 0 0.33 0.33 Rabbit G117 Rabbit 0118 2.260 - 9.1 - 5.2 0.33 0.33 Rat 437 Rat 438 . 0.175 0.205 - 5.7 0 - +57.3 +32.2 -19.5 +20.8 + c.4 16.7 4* i. ^ + 3.5 +1.. .5 15.7 +32.9 7-3 20 .4 2ied Gied survived Survived Survived Survived survived survived Survived survived survived survived Survived :urvived Survived survived -- 22 days X 19 days X 12 daya 0 28 daya S 1.1 aontha X 1.1 aonths X 8 days X 8 days K 11 daya.K 6 daya S 1.1 nontba X 8 daya X . 0 daya X 8 daya X ; i's'olc 9 (tu; e 3} CO.NJF 2xporlaont Kuraber Duration of Exposure flours Identlflea tion Nursbar Initial ,,'oijat k- Chan;:e in ..eirnt toprecced e s Percenta; e of Initi al Weinht i>UTtn Period cf exposure lifter Period cf Abipocure l ate as '.died, at Of Last . i^riod of -i^pocure Len.i f. of survival of Aniraals fbat lied (D) or Vere Killed (X) After boat Period of sxposur. 6 3-5 Guinea Pi? AcJi6 1.060 0 + 6.3 Guryived 7 days X. 3-5 Guinea Pl AoJs.7 C.770 -7.3 +13.2 burylved 7 days X 3-5 2 Xice ) _ -- 2 Oied -- 3-5 2 I-Sice ) Ay.0*024 -- -36.0 Survived 7 day* D 3-5 1 House ) a. - Survived 7 days X 3-5 Rabbit G129 2.23i> -0.2 - 7.0 Survived 7 days X 3-5 Rabbit G130 1.V20 0 + 0.3 Survived 7 days X 3-5 3-5 Rabbit G131 Hat Vjji; 1 1.965 0.315 O - 5.2 a. Survived Oied 7 days X 3-5 Rat K'jJi-P. 0.335 -7.5 + 9.6 survived 7 days X ? 1.0 Guinea Pit; AK*it 0.055 0 ** 2.1 Survived C days X 1.0 Guinea tie; AGttS 0.070 0 + 3.1 Survived 6 days X 1.0 5 Xice Av.0.023 -6.0 +16.5 survived 0 days X 1.0 Rabbit C119 -0.8 +19.1 Survived 25 days X 1.0 Rabbit 0120 2.325 0 10 & Survived 5 days K 1.0 ' Rabbit 0121 2.W0 -7.3 +35.8 urvived 1.1 nonths X 1.0 Rat Eb39 0.195 -5.1 Survived 3 days X 1.0 Rat RljiiO e.190 0 +23.7 survived S days X HONS 0 5 6 0 3 9 gable 10 f- i C- i K TI A L The ."iortallt:/'. Ur. th cf -arrival, and Chcnres In Vlei-ht of Aniaals Exposed to Aerosols I or-xed by Dro,r_.ir,-~ Tricro?'/! ihosphate Into an Inoonel Tube Heated to1 a PeT**>orgi.ure of 1050 P (566 C.) Exper Duration of iment iixpcsvir Identific&t Icn Somber KoUZ*S Slices* Initial Geirht '*+i. Change in Ooi^ht Exproesod as Percentage ol' Initial Height during Period of Exposure After Period of Exposure Pate as noted at End. of Last Period of Exposure Length of Survival of Anbaals fiat Died {D) or Mere Silled (K) After last . Period of Exposure 7.0 7.0 7.0 2 x 7.0 2 X 7.0 2 x 7.0 7.0 7.0 Guinea Pig Ac60 Cuinea ri^ Ao61 5 Kice Rabbit G163 Rabbit Gl6i* Rabbit 0165 Rat El*71 Rat 4*72 1.035 0.563 Av.0.0208 2.393 2.029 2.005 c.J*54 0.474 - 4.S - 6.0 -23.1 - 1*.S -12.3 -10.7 -10.1 - 5.9 . Died - Died - Died - Died a. Survived - Died - Died - Died -- a. _ 1 hour D - -- , - . - . 13 7.0 Guinea Fir AC50 04*30 - 2*.2 Died -- 7.0 Guinea fir. AC59 C4*10 - 1.2 - Died _: 7.0 3 Mice ) ._ . - Died _; 7.0 2 race AT eVaOlO } a Survived 13 <Jay S : 7.0 Rabbit 0151 2.01*0 - 7.7 +16.9 purvivod 12 day* K . 7.0 Rabbit 0152 2.525 ID .5 +13.5- Survived 13 day* X . 7.0 Rabbit 0153 2.035 - 6.2* a. Died * 7.0 Sat l*63 04*70 - 4.0 - Died --; 7.0 Bat KE*6* 04*55 - 2.2 " Died *; 0 V 0 9 S 0 SNOW i Table 10 iPa;e 2) CC ; Exper Duration of iment Exposure Suaber Hours Identification itasber Initial Weight Change in Weight Expressed as Percentage of Initial Weight Daring j Period of { Exposure After Period of Exposure Pate as Uoted at End of East Period of exposure Length of Survival of Aniaals Chat > Died (D) or Wars .Killed UtJ' : After East . Period of Exposure ; 3 10 x 3.5 3.5 3.5 3.5 3*5 3-5 3.5 3.5 3.5 l.o 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Guinea Pig A0l8 Guinea pig A819 1 House ) 3 Mice ) Rabbit G17 Rabbit Gl8 Rabbit 019 Rat B393 Rat 53%. oo Av.0.0138 2.150 i.ec4 2.180 0.193 0.197 Guinea pig Ao52 Guinea Pig A853 3 Sice ) 2 Mice Rabbit G142 ) Rabbit Gl!}3 Rabbit G144 Eat 2454 Rat E455 1.110 1.280 Av.0.018 2.166 2.835 2.W0 0.160 0.160 - 1.8 - 3.3 -19.3 - 1.6 - 2.5 - 2.8 - 4.1 0 -11.1 -22.2 - 2.0 - 5.0 - 6.9 44-9 -IV-0 +23.0 12.2 20.7 12.7 3.0 - 3*8-8 19.5 -47.8 -43-0 + 6.7 19.4 * Died Died Died Survived Survived Survived Survived Survived Survived Survived Survived Died Survived Survived Survived Survived Survived Died 26 days X 7 day* D 20 days X 20 days X 25 days X 25 d*y X 6 days x 2 days 9 as 7 days X 26 days 9 17 days 9 8 days X 9 days X > i i 1 I1 i 1! 11 i 0.33 0.33 0.33 0.33 0.33 o-33 0.33 0.33 Guinea Pig AS54 Guinea Pig A855 5 Mice Rabbit G145 Rabbit 0146 Rabbit C147 Rat g*56 Hat S457 0.446 0.4.65 Av.0.0190 2.400 2.333 2.395 0J1S0 o.45o - 5.5 - 1.7 -13.7 - 2.2 - 6-4 0 - 6.0 - 9.1 15.9 5.6 +17.9 + 3.5 11.6 10.9 2.4 17.8 Survived Survived Survived survived Survived. Survived Survived Survived 9 days X 9 days X 10 days x 3 days x 8 days X 8'days X 9 days x 9 day* X iONS 056041 ! 1 n HONS 0 5 6 0 4 2 Exper Duration of iment Exposure Identification Sunber Hours i-unber Jable 10 (2a^o 3) Initial height *e- Change In Velght Expressed as Percentage of Initi During Period of exposure After period of iLxposure CGX ! Rate aa Soted at Last Period of Exposure a; Lang tb of SvorvivaX; of AnlvlrThst '? Died (9} or- Mere ` Silled (XJ >= After last 1 ' Period of 9 3.5 Guinea pig a850 0.920 - 3.0 0.9 Survived 8 days X - .. 1 3.5 Guinea Pig A851 1.035 3.5 3 Hies 3*5 2 Mice ) ) AV.0.C17 3.5 Rabbit 0139 2.145 - 0.2 -11.9 -21 Jj. - 9.0 -_ - 21.4 6.3 Died Died Survived Survived 7 days X 9 day* X I 4 3.5 Rabbit C-110 2.430 - 4.9 0 Survived 34 days X 3.5 Rabbit G141 2.460 0 + 0.8 Survived 4 days X 3.5 Rat E444 0.360 - 6.7 a. Died 3.5 Eat H|45 0.330 -10.6 8.8 Survived 9 days X 12 1.0 Guinea Pig a856 0.750 - 5.2 + 1.2 Survived 8 days 9 1.0 Guinea Pig A857 0.510 - 5.3 -17.6 Survived 29 days 9 1.0 5 Sice At.0.017 0, 11.8 Survived 1.0 Babbit 0148 2.686 - 8.1 10.2 Survived 9 days X 8 days X 1 1.0 Rabbit 0149 1.0 Rabbit 0150 1.0 Rat E458 1.0 Bat 459 2A00 2.170 0.460 0.430 j - 2.0 - 1.6 - 4.8 - 6.5 16 .4 15.2 + 3.0 4.9 Survived Survived Survived Survived 8 days X 23 days X 9 days X 9 days X i Vls l 1 ie'clc 11 (i-a^e 2) CC Compound and Xeaperature of Contact lacrstlon Uours .aco Ka ts Guinea Pi3 Saobits Cate i^orinent Itasbeaf Jtroclor Decorqxjsitlon 1050 ?. 7.0 * 7.0 7.0 3.5 1.0 0.33 3.5* 1.0* 5/5 f/5 % 2/2 2/2 0/2 0/2 0/2 0/2 0/2 Trieresyl Phosphate Docoapositlon 1250 P- 2x7 7.0 3-5 1.0 0.33 3.5* 1.0* 1 2/2 2/2 0/2 0/| 0/2 zfe 0/2 1/2 0/2 Trieresyl phosphate tecoapositlon 1050 >- 2x7 7.0 3.5 1.0 0.33 3.5* 1.0* 5/5 3A iA 3A 0/5 3/5 o/5 2/2 2/2 0/2 1/2 C/2 1/2 0/2 s 2/2 */* i/2 2/2 Z/Z 2/2 0/2 0/2 0/2 2/2 2/2 2/2 l/2 C/2 1/2 t/2 8 X%U? & "4oA # 3/3 1/3 1/3 2/3 o/3 V 0/3 - c-A . _ - 8 7 9 10 11 12 13 15 2 1 It 6 8 7 lit 13 3 10 11 9 12 ` Sate of liquid delivery use about os*--half of that in other e:c;erifsor;t of similar duration- HONS 0560*3 1'afcla 11 CO i'-' ' l A L Suaaarr of Mortality Aincn--- Groups of Anlnals Subjected to Aerosols Foraed by Dropping Pydraul F-9. Aroclor X2kS or grlcresyl Kiosshate Into a Heated Inconel Tube -4 q =j --v**i ..I Compound and faaperatur* of Contact Pydraul Decomposition 1250 F. Duration Bours Klee Bats 6.0 6.25 6.75 3.1 0.9 0.25 1 2/2 2/2 2/2 0/2 0/2 Guinea Pigs Rabbits V2 2/2 1/2 1/2 0/2 0/2 0/2 1/2 0/2 0/2 Cats 0A A oA oA Experiment -j ' ' J| I '1 k3 31 s .i ti $Pydraul Decomposition 1050 F. 6.5 6.8 6.8 34 0.9 0.25 8 1/2 1/2 0/2 0/2 0/2 2/2 2/2 2/2 2/2 0/2 2/2 8 oA Aroclor Deoesposltion 1250 F. 7.0 3-0 1.0 0.33 3.0* 1.0* 2/2 i 2/2 0/2 0/2 0/1 1/2 0/2 2/2 2/2 2/2 2/2 2/2 0/2 8 $$ - i 9 10 1 2 6 j 1 A "i j ; i i ii HONS 0 5 6 0 4 * CONFIDENTIAL Tabl# 12 .Tncldewoo of Mortality In Relation to Haterlal 'Torn! oraburp of fog) T-noonol Spolo* Total Humber c>f AnirrmX# That Died/iotal Humber ixpooed Iydraul F9 Aroolor 124 Triorosyl Fhoephat# Mio* t 28/49 (S>71) % 37/63 (SB.7) * 32/69 (46.4) Guinea H(;, 13/20 (6S.0) 24/26 192.3) 17/20 (60.7) Habbite 4/?4 (10.2) 16/30 (42.1) 9A2 (21.4) Hat# ' 6/20 (40.0) 9/26 (34.6) 11/20 (39.3) HONS 056045 CONFIDENTIAL mulu, jtnoMenog-.of Spool--V and Toni-iefoturo of Inconol .''pools* Mloo Oulnaa I'lffa Uabblta Unto Total ;Uo* Quine* Tl(' Kebblta riot* Total Mlot Oulna* Tigs Httbblt* Oat* Total Number of Axilme.In jhot Ulod/llumber of Animal* Fxpoaed 1250 F. 1050 F. Aroolor 1248 % 24/29. (62.8) 10/12 m (i6s3?.3! 46/71 (64.6) * 13/34 (38.2) 14/14 (100.0) 9/20 (45.0) 4/14 (20.6) 40/02 (40.6) Tydraul F-9 15/24 5/10 1/10 6/10 * (62.5) (50.0) (10.0) (60.0) 27/54 (50.0) 13/25 0/10 3/14 2/10 t (52.0) (00.0) (21.4) (20.0) 26/59 (44.1) Trloreayl fhoaphate 17/3? 7/14 2/21 5/U % (40.6 (50.0 < ?.5 (35.7) 31/84 (36.9) 1105//1314. I0/llt % (niil I8:il 30/63 .(45.8) HONS 056046 HONS 056047 i'a'oltt 14 The Toxicity of fj-uraul I--9 when Hal; tai-.ea Coriinncu.slT for TuantT-fo-r Sours ir. Contact With the Akin of :;nbtlt3 by the Sleeve "othod of Praise. ioodsrd and Cslvory (Applied undiluted) Identi fication Condition Ihrater of Skin Initial '8L>t Loss of '..eight impressed as rercenta.a cf Initial WaisJht jf ate Length of Survival After the Material fas Applied c-333 0-339 Intact Intact 9.4 2.06-3 9.4 2.390 0-340 G-419 0-421 Intact Intact Intact 6.0 2.275 6.0 1.630 6.0 2.016 0-420 0-436 0-439 Intact Intact Intact 3.6 2.100 3.6 2-262 3.6 2.265 0-455 Abraded 6.0 2.090 g-45? Abraded 6.0 1.9S6 g-458 Abraded 6.0 1.666 36.1 17.4 19.8 9.9 9.6 6.1 . 3.6 3-7 36.5 4.3 li.2 died Died 7 days 3 days Pied/ 20 days Survived 9 days; killed Survived 9 days; killed Survived 23 days; killed Survived 16 days; killed Survived 16 days; killed Pied Pied died 19 days 44 hours 3 dsys i 1) i i Table 15 nt:al The ~oxXslt7 of Aroclor 12LS When Maintained Continuously for Twenty-four Hours la Contact Kith the Skin of Rabbits by the Sleeve Method of Praise. Woodard and Calvery . (Applied undiluted) Identi fication Kuaber Condition of Sirin Dose al./fc. Initial Weight leg. Loss of 'weight Expressed as Percentage of Initial 'Weight Fate Length of Survival After the Material Waa Applied 9*0950 SHOW II! EEg G-3bS G-tfUO c-4i Intact Intact Intact 9-4 2.>10 `>4 2.310 94 2.053 0-422 Abraded 94 1.C65 G-424 Abraded 94 1.922 c-443 Abraded 9.0 1.970 G-liUh Abraded 6.0 2.196 0-456 Abraded 6.0 1.662 19.3 3.9 26.2 9.2. 35.2 14.8 19.2 12.7 Survived Survived Survived Survived 4 day*; killed Died " 8 days Survived 29 days; killed Died Died 6 day* 21 hours Table 16 ' T -- fvj T the .`o.-loltT :,f Xrlcrorvl rh&ay'sgto 'when MEintair.sd Continuously For Twenty-four Hours In Contact With. the Skin of babbits by the Sleovo Method of Pralao, Woodard and Calvary (Applied undiluted) Identi fication dumber Condities of Skin Dose xal./kt:. : loss of Vieiglit Initial iiepressod as Weight : Fercentage of k. 1 Initial Weight Fate length of Survival After the Material Was Applied 5V09S0 SNOW 0-628 G-337 0-334 c-m 0-345 c-423 0-!;42 0-445 Intact Intact Intact Intact Intact Intact Intact Intact 9.4 6.0 2.5 2.5 2.5 1.6 1.6 ` 1.6 2.487 2.460 ! 2.190 ! 2.360 2.125 | 1.620 ; 1.609 1 2.158 i 15.5 16.1 21.0 38.2 9.8 13.2 11.3 5-9 Died 2 days Died 2 days Survived 20 days; killed Died 7 days Survived 6 days; vmad Survived 9 days; killed Survived 21 days; killed Survived 16 days; killed -c.'clo 17 ; ' I c 3 N TI A L the Toxicitv of Car-oyle CT jl V.nen 4e*.-.i-aiLr:c<I Cvoici/uc? .-or Tventv-focr Hours in Contact with tlvo s <cia of :.ab uits by the Sleeve ' ;ethod of Dr&ice. .loodsrd and Cu-lvery (Applied undiluted) Identi fication Condilion Suraber of Skin Dose nl./k> Initial Weight Loss of vS.* _iit Stressed as ie-rcontce;o of Initial Wei;-ht 1 ate Leu: th of Survival After Uiis Sa Serial as Applied 0-329 G-33I 0-341 0-342 g-343 c-332 0-352 0-354 Intact Intact Intact Intact Intact Abraded Abraded Abraded 9.4 9.4 5.4 9.4 9.4 5.4 9.4 94 2.470 2.345 2.C30 2.210 2.160 2.040 2.450 2.255 12.9 11.9 15.2 17.6 1C. 2 6.6 10.4 20.2 Survived surv ived survived Survived Survived 14 days; killed 18 days; killed 20 days; killed 19 cays; killed G days; killed survived days; killed Survived 20 days; killed survived 20 days; killed 0S09S0 SNOW I asai&Jg -ONf`Dnt;al Suaaarizod Seta os tlio I*-;;,oalatcS- Tonicity of Pydr&vl r-9. Aroclor 12hS. Trlcrer?! Phosphate ar.d Gargoyle DTE -has Maintained ~0aon the Skin of babbits According: to the Sloeve :jothod of Jralze. Woodard toad C&lrery MONS 0 5 6 0 5 1 Blle/kg. JhEibor that 2ied/Wuaber Given che Material Pydraul ?-9 Aroclor 12ij.8 Tricresyl Phosphate Gargoyle 3>IK Intect Abraded Istact Abraded IStact Intact Abraded 9.4 2/2 0/3 1/3 VI o/5 C/3 6.0 V3 3/3 2/2 1/1 -- 3.6 9/3 - -- - -- 2.5 - - - - 1/3 - - 1.6 * " ' * - c 0/3' -~ Figure 2 HONS 056053 II Figure I i ! I Figure 3 HONS 056054 Figure 4 HONS 056055 Figure 5 W Figure 8 Per cent Transmisson Figure 7 Per cent Tronsmisson 100 .1 0909SO SNOW -J Per cent Tronsmisson 3 O 2 O \n O' Figure 10 O' Jh.. FIGURE II. HONS 0 5 6 0 6 2