Document o9v3YYjZK5bG0keRx5Mxd7eqX
AR 226-082
MICROBICS MICROTOX TOXICITY TEST
TEST SUBSTANCE
Identity:
ArefmeirxrteudretocaonstPaiFnOiSng,
pFeCr-f9l5uo,roorocatsanaesculfoonmatep, woohfinFchC-em2a0ny6CatEl.so
be (1-
Octanesulfonic acid) (CAS # 2795-39-3).
R20e6mCaEr.ksC:urreTnhtein3fMorpmraotdiuocntiinodniclaottensuimtbisear wmiaxstu5r0e2.ofT0h.e92t%estPFsOamSp,le15is%FCd1i.e5t5hy%lsenoedigluymcoolctbyultysluleftahteer,,17.60.50%9p%owlaytoexry,et4h%yleurneea,mo1n.o2oc8t%ySluplhteonnyel feotahemre,r, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole.
cThhaerafcotlelrowiiznegdscounmcmeantrryatapipolnisoesftiompaumriitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately
reflect the toxicityofthe fluorochemical `componentofthe test sample.
METHOO:
Method: Microbics Microtox Procedure
Test type: Static acute
GLP: No Year Completed:
1989
ASnpaelcyiteisc:alPmhoontiotboarcitnegr:iupmHphaonsdpohsomroetuimc condition.
Exposure Statistical
pmeertihodo:ds3:0-EmiCnsutveaslues
calculated
by
statistical
linear
regression
pTersotgroarmgparnoivsimdesdofuorrcMei:crMoitcoroxbics' Reagent.
Test coDnidliuteinot:ns:Microbics 2% NaCl solution.
Stock Taensdttteesmtpseorlauttuiroen:prNeoptarnaotteido.n: A primary stock solufion was
prepared in an osmotic
aMdijlluisptomreenMtiwlla-sQTMmawadteeru.siTnhge20p0HomfgthNeaCsltodciksssoolluvteidoninw1a0sm6L.7
and
smtioscckibsloeluitniwoant.erA,ppneoacroalnorceapopfarteesntt"s.oluAtliotnesstwsaosluntoitonesdwaesr"eapmpaadreentblyy
pErxoppoorstuironealvedsisluetliso:ns.4 mL cuvettes.
Number of replicates: two Number of concentrations:
Four plus ablank
control
--
Element basis: Percent light loss
RESULTS
Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L.
001749
Element value:
155--mmiinnuuteteECEeCoso==654050(5(94020----71409)0)mgm/gL/L 30-minute ECso = 420 (390 --440) mg/L
Element values based on nominal concentrations.
Remarks: Substance
TReestmianrgkwsafsielcdo.nTduhcetveadlouensarmeipxotrutreed
aasppdleytscortihbaetd
miinxtthuereTeasntd
not
the fluorochemical proportion alone.
CONCLUSIONS TdehteerFmCi-n2e0d6tCoEbe304-2m0inmugt/eLEwCistoh faor9P5h%otcoobnafcitdeernicuemipntheorsvaplhoofr3e8u0mtwoa4s40 mglL. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St.
Paul, MN, 55133
DATA QUALITY
ca
--
-
RHeolwieabvielri,tys:amKlpilmeispcuhrirtyanwkaisngn2o.t pTreospteirnlgymceheatrascatlelrcirzietderiaanfdortqhuealsittuydtyesltaicnkgs. analytical confirmation of the amount of fluorochemical proportion in the
`solution.
REFERENCES
TSth.isPasutlu,dMyNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMG1C3o5m8p-a2n,y1,98E9n.vironmental Laboratory,
HER
--
--_--
Last changed: 6/26/00.
0017590
.
BEST COPY AVAILABLE
CMVIFOMENTAL LABORATORY -- MIcROTOR SYSTEM
RAM DATA
wel3sr-2
1 Fa 3 ?
Test susstanee: EC ROLCE LotSOA
TEST DATE:,
--+
ANALYST: goes 2 Bene,
SAMPLE CUVETTE [rnsonconor mga
o=
N
| 10%|
5-MIN. 1(5]
-
FRC RERDINGS
15-MIN.. 30-MIN.
15)
30;
a
DUPLICATE
u
as | 77 7A 1i 619
82
125
go t3 |53 | 8)
2
Duplicate
96 69 | LO 57
&a
DuSplricate
W2s| 55]4 | 479 iHo"
8
S500
97 | 44 [34 |3!
2
Duplicate
/0| 47 |37 |39
8
l000
96 | 3] |aax |/9
es
wplicate
92 24 |&\ | 18
TestSubstance Stock Solution & Zoomg
Yelow Ligpid
Each. Lomi > 2.0m:
joond.Milli-Q wake .
smoteAdysinent 3SiLe0t0e0Go20m0amnat, MeacSk d,o lnooncodleSaapmpparleent:
-
Oilvent:
Micrbies 27 Mack Solution
Thisial Dilowone2 Tnisial Concentrations Were. dooble. +he Final
JSeoreeyd CercentruP hions HeL nsRiIonRed AA bSoveE: vDueN SiInaBlEdT ilepnioines
.
Lk G1358-2 FC-206CE
MICROTOX (1) DATA SHEET S-MIN EXPOSURE 6/16/89 SAB
raAepH
:
:
L
PAIR * --_--T z3
a5
67
&
BLANK
BLANK
EC 50
cone. 125.m6a0l0 l
To/1t 90-- 707 63.0
5-085 _5.188
gst pGlast
Pement hight TWLosT s T
122550..000000 250.000
19060..00/7 5699..00 98.0/ 59.0
00..132784 0.3%
00..135313 0.333
500.000 S70 24.0
0.787
0.735
aiw7
*
2B6 on
5%0100.0000.0000 101.9067.0747.301.0 0.17.45210 0.7i3e521
0 a]i
%1000.000
92.07 29.0
1.571
1.621
l
RBAoTsIBOe==0."8915057 77..nt".. BEST eCOPY. AVAILABLE G>r_eGmotsiftofp
EERIE ?Eh
Sle TL TT AR RE
='o &51,251 (35%93.503 TEO o714.617 ) bso (590 i 7/0) mally SEE fd
R =0.99557 SLOPE = 0.8691 INTERCEPT = +6.4789 =
:
4+10.1RE1S9. :Zz 0o..103736 L5 o0..o07882 &7 o0.0z80 & 0.19
yo . :
Listc 2oR0NN FFEN, kK
PROB8.0
[ : UPUREA|
t
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1
10303 ::
:-
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7at7. ;
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1[I
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FRE
:3 :
mmme
C1 a
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a
ial
3i 1s1
=
:
eaSoemme C3 gh EEER m2 EL Be a
ca
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P. E
RR von
[ t P --2---mn EE :oh I
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T 3& L,oo" ALP DoT" 1
47 5T AR VvoO EN M8 T RO5: os REN
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001752
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LR 1358-2 FC-206CE
MICROTOX(r) DATA SHEET 1S-MIN EXPOSURE 6/16/89 SAB
PE3og YH
i gi Hh
PAIR #
cone.
male
lo/1t
6-088
G-gsT
Parcent Ligh, .
Lost:
251:
215122055...000000000 250l000
.
1990060...000777 654038...000 98.07 49.0
000...522718937 0.516
000...522355022 01530
v 3#37. Fo, i ihA
s. 505000.,000000 19071.,00/7 3374..00
11l106629
tThHs
:5! :
7 71000.000 96.07 22.0
2.307
2.339
70
a
%1000. 000
92.0/ 21.0
2.320
2.339
70
BBLLAANNKK, RBAToI/OB:t=0.975573/ 72 BEST COPY AVAILABLE Crceogmesmn [MP t|y
:
Ln
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TEE
gECSOg-= m451.134 C 4u1s5:.191 TO a490.189 ) 4se (420-: 490) wale]Boh tinE g
R =0> .99476 SLOPE = 0.9259 INT-ERCEPT = 46.1118
bE sda
Child
4%1 oRnezs.e FROB7.1
1 A : m mmm m m e m n o iFhE Lemp
: ones 100
a_i
53i 00o..l00o88m48s I3337a,n :
- sC imm m if[I ou
57 olohsms 3 ohm
e"t os: ez -
--tm
<: s
J
"ile
----
L1AN
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irrae n
rr
L: n
Sra
PHaom
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Sdiak
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BHYiaoasnli
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rast
e1LIeST EF NF
n2RTUN 63
KE
"a
L L Oo e A ODT" a
Y
S LOG CONC.
6
7. Ri
S4 TsRA oV NE EB TR TOE LER 0 s c R E.LfGgE
8
Sm
TDD Zine
= ev == ee
1
+
shee
=e
-
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eel
01S
Pa Hop Hil
MICROTOX RG EOPYAVAILABLE
LK 61358-2 FC-206CE 30-MIN EXPOSURE 6/16/89 SAB
J
is
PAIR # conc.
1 125.m60y0
z 125.000
2:s 225S5o00o...000000000
s
500.000
87
#%11000000..0O0O0O,
Taste
9960.706/7 5517..00 1990780...000777 433816...000
101.07 34.0
9962..0077 1198..00
6-088
05..222832 001..265714347
1.158
22..677102
G-esT
05..235555 00..1552366000
1.230
22.,669988
Feraps Lights ALiogRssTTR
SE333y5
S54
7233 cut td
BBLLAANNKK RBAoT/IBOtS=0.95727.636&9 :
ECCSO SO == 416 416,92 id (3(903.9008.4084 TOTO 484.4. 047)
ST Se) 6-088 xfeo"
YA0(390-40)
mal3 tPHTa=HEhut
i ;
R 0.99444 SLOPE 'm 0.8762 INTERCEPT = +4,Q311
SH
#41 0R.E1S0.0 :3 o0.u06d7 54 00..007m0 S>s 0a.l0t6o8s soos
-
ten
FROG7.6
:.
13050 ;
88 :
Ices or
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i
i:
CE as:
:
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:
a pda aad fiegm Sg
. r8e-1
mri i fi
ea nn
vsi ii.
Sfyy,ee
<iit8e
------r
ay
i m-Famem leone
ey a
PE
s Viren rCieADLing
2emir
T: eh : Fdriieged
tom
:
i
2
ty
Pid
22 :mmm 5 Losecom c. se mimi=IA
IST 2RUN
3 L 0 AD"
eFonx EY . Cc ONT & , " L PT 1
4 8A V E
7 o T Rs.OcN
8 rR T
5
E RQE 1
-
001754