Document o9OJx4gwqjnYy5vdV5n9X89Vr

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95 or as a component of FC-201 AF. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted. The testsample isFC-201AF. Currentinformationindicatesitisa mixtureof 3% PFOS, 18.59% diethyleneglycolbutylether,37.81% water,14% ethyleneglycol, 8.25% Acrylicfoamer,9% sodium octylsulfate0,.3% sodium laurylsulfate, 3% triethanolamine6,% sulfonatedalkylethersalts,and 0.05% tolyltriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentrations of impurities.Date may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline203 Type: Acute static-renewal GLP: No Year completed: 1995 Species: Pimephalespromelas Supplier:AquaticBiosystems,Inc.,FortCollinsC,O. Analyticalmonitoring: Temperature,pH, and dissolvedoxygen. Exposure period:96-hours Statisticamlethods: LC50valueswere calculatebdy theTrimmed Spearman Karber method usingthe EPA ProbitAnalysisProgram, Version 1.5. Test fish age: Juveniles (-89-90 days) Length and weight: Average length 25 mm Average weight 0.109 g (wet) Loading: 0.607g/L Pretreatment: None. Test conditions: Dilutionwater: Carbon-filterweedllwater (3M #2, St.Paul,MN) Dilutionwater chemistry: Not given. Lighting:Not given. Stock and testsolutionpreparation:The testmaterialwas added directly to the dilutiownater on a weighvvolume basis. Concentrations dosing rate: Dailysolutionrenewalduringthe study. Stabilityof the testchemical solutions: Not noted. Exposure vessels: 2 L glassbeakers containing1.8L testsolution. Number of replicates:2 Number offish per replicate:10 Number ofconcentrationsf:iveplusa blankcontrol Water chemistryduringthestudy: pH range(0-96hours): 8.0- 8.4(contreoxlposure) 7.9- 8.3(320 mg/L exposure)* Temperature range (0-96hours): 17.6- 19.8OC Dissolved oxygen range (0-96hours): 6.1- 8.5mg/L (controelxposure) 2.1- 8.5 mg/L (320 mg/L exposure)* 320 mg/L (intermediatceoncentratione)xposure valuesused because the highesttwo concentrationsresultedintotalmortalitbyy 24 hours. RESULTS Nominal concentrations: Blank control1,00, 180, 320, 560, and 1000 mg/L Element values: 96-hour LC5o = 310 (270 - 370) mg/L Element values based on nominal concentrations Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapply tothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hour LC50 forfathead minnow was determined to be 310 mg/L witha 95% confidenceintervaolf270 to 370 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY ReliabilitKyd:imischranking2. Testingmeets the critedaforqualitytesting. However, sample puritywas not properlycharacterizedand the study lacks analyticaclonfirmationofthe amount offluorochemigalproportioninthe solution. REFERENCES This study was conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number N2964, 1995. OTHER Last changed: 6/2600 a m::!c e ErivironmentalLab Work Sheet e s, N c ci -------------- F@ LIN T At ILI --A: lie: i A/ Environmental Laboratory LR a e C/ 9&Hour Acute Toxicity To Fathead Minnow, Pimephales PrOme[3-S L,-.)adingRate Data Sheet T5@stSubstance: -FC A klinnow I 23 We_ti.Inlhht, -T---I(, FLLLeennglth,(cm) @1 -7 3 4 5 6 L4 7 8 9 10 Mean Av q ()7r9 C),CCqZCC4,4 T-7, C, Loading Rate CaIgulaUon Mean Avg. g. (n) g/l.OL (@/1.BL- (n) g= C,(ct'T-- Loading Rate= g/L. "it IT- Environmental Lab Work Sheet Z;ace ate: No 1)2 0f c IT EPJ"RONMENTAL LABORATORY LR '2q(41 96-HR ACUTE TO)CICITY TO FATHEAD MIN?40W, PIMEPHALES PROMELAS T)'PE TEST: STATIC X STATIC FIENEWAL TE.ST SUBSTANCF-* NaCt, granular,CHEMPURE BRAND ACS, LOT M272 KPKC DILUTION WATER- CARBON-RLTERED WELL WATER (3M WELL 92,ST. PAUL, MN) Tomp.__@@@ -C. pH 'S. D.O. -, .. mgll TI:ST FISH SOURCE: Aguatk B[oSyrten-m,lnc- FortCoiliiu4CO FISH AGE: C,- -1 AVC, WT- N D. OF RSK(TEST CHAMBER. 10 Ti-ST SOLUTION VOLUME: -1.8L DATE lPdTtATED* i /I(L-Ci't7 DATE RECEIVED: AVG. czx7F- 12C O.IcTn LOADING RATE- SIZE OF TEST VESSELS: -2-0 L DATE TERMINATED: TEST INITIAL TOTAL IVESSEL CONC. AMOUNI 24-HR pH cWL ADDED' BK1 CONTROL BK,2 CONTROL I-A 7.5 13.5 C-. 2-A 7.5 13.5 1 -B 8.7 15.66 2-B &7 15.66 I-C 10 2-C 10 ls@"@ C- F" i' i-D li@5 @'2'D.71, to 2-D 11.5 20.7 -Y I-E 13.5 24.3 2-E 24.3 f C,, -7. D.O. 484iR ffg& MORTALITY PH D.O. 17-2-HR PH I mcYL I :Q. I .7 -Z.7 fMfOYx.L 964iR PH (- 14 D.0, M91L y IW t rEM P.IC :)ATE ANAL YST@*,,4 LCSO(95% COMME d .;'AN um: Q-- chiiescentR EffabcSwknn*u H Ha-m ENVIRONMENTAL LABORATORY 96-HR ACUTE TOXICITYTO FATHEAD MINNOW, PIMEPHALES PROMELAS TYFIETEST: STAiric STATIC RENEWAL TEEIT SUBSTANCE: FC-201 AF DIL LMON WATEFL- CARBON-RLTERED WELL WATER (3M WELL #:2@ST. PAtJt, MN) T*m p. iq -L- IC,pH MO. 91 L TEE;T FISH SOURCE. FiS[iAGE: AQUA-NC BIOSYSTEMS, INC FORT COLLINS, CO. AVQ WT-: NO OF FISRrrEST CHAMBER: 10 DATE RECEIVED: 9 AVG- SJZE- LOADING RATE. TE!;T SOLUTION VOLUME: SIZE OF TEST VESSELS- DATE INITIATED: DATE TERMINATED: le-l@ c LR N2964 cm 0 L BEAKER Tlz-ST INITIAL TOTAL @:,-#AORTALFTY,' VE S@SE L CONC. AMOUNI 24-HR pH D.O. 484iR pH D.O. 724iR pH mg/L ADDED' I MGVL ffKYL lWl CONTROL IOQ CONTROL I-A 100 ISO ?-A 100 180 l-a ISO 320 c C- 11.4 2-B ISO 320 -C 580 1 2-C, 580 --7 I-D 560 1000 - 2-D 560 1000 1000., 18W Ir 2-E," 1000,- '1800 79 c D.0- 964iR pH D.O. MCYL 4 El TEM 'ID tTEf/iANA to 9 7 LC;50'(95% C.)1" %A)MMENT I D 410 -,@go- Lp 11 Nvnl A'LWEIGHTS. mgA-. L4 &n Surtac*)%E l@dssofEqtAbrkm; Qt4:oulascon R Effaft Herrooffhadno