Document npq4EwBZ4B1BV2MgVbv5GB6ZG

MINUTES AROCLOR - WILDLIFE; REVIEW OF BIO-TEST STUDIES MONSANTO GENERAL OFFICES MEDICAL DEPARTMENT ST. LOUIS, MISSOURI September 19, 1969 TO: Those Preaent M W. Farrar w H. Hunt R E. Keller R E. Kelly E S. Tucker P. Wheeler Not Present W. R. Richard The above attendees reviewed with 0. E. Fancher, Director, Industrial Bio-Test Laboratories, the biological results to date of their feeding-studies and the consolidation of tissue samples for residue analysis by the Analytical Group of the Applied Sciences Section. MQNS 031382 -2- A detailed analysis of the currently planned analytical support work for the Aroclor - Wildlife project was prepared for this meeting/ Table I. The analysis includes a listing of studies, manpower requirements and a dollar cost for the analytical support of each study. In brief, it was ascertained that there were at least five separate Bio-Test feeding studies in progress or planned whioh would require 105 man weeks to analyze the estimated 1340 samples being generated, at a cost of $63^000. An additional $18,000 of work not involving Bio-Test is also outlined in the Table. Meeting Objectives I. Review Bio-Test feeding studies with special regard to the scheduling and reduction of the number of samples to be analyzed. II. Status review of the biological results, to date, of Aroalor - Wildlife Bio-Test feeding studies. , With these facts in mind, each Bio-Test study listed in Table I was critically reviewed to determine if the number of samples . requiring residue analysis could be decreased without appreciably effecting the results. 1. Rat Tissue Study Actions: - Delete residue analysis of rats fed chlorinated.naphthalenes and pesticides. Fool male and female animals. 2. Chicken Toxicity, Reproduction, and Meat and Egg Residue Study Actions: - Fool larger numbers of eggs. - Analyze only fat, muscle, and liver tissues. - Restrict analysis to one, no effect level for each Aroclor. 3. Mallard Duck Study Action: - Equivalent to Study No. 2. 4. Two Year Chronic Toxicity in Rats Actions: - Delete 9 month sacrifice. MONS 031383 -3- Actionsi (Cont'd) - Pool all male and female animals. - Restrict analysis to one,no effect level for each Aroclor. 5. Pish Toxicity Study Actional - None) study design requires this number Of samples. 6. Rat Reproduction Study . Actions! - Addition of 2nd litter, 1st generation, rat pups to be analyzed. 7. Two Year Chronic Toxicity Study in Dogs Actions! - Addition of selected tissues for residue analysis. TABLE II AROCLOR - WILDLIFE ANALYTICAL SUPPORT REVISED (Bio-Test .Section Only) Mo. Samples I. Residue Determinations A. Biological Residues (Bio-Test) 1. Rat Tissue study 2. Chicken Toxicity, Reproduction, and Meat and Egg Residue Study 3. Mallard Duck Study 4. Two Year Chronic Toxicity in Rats ' 5. Fish Toxicity Study 6. Rat Reproduction Study 7. Two Year Chronic Toxicity Study in Dogs 40 96 96 160 60 10 40 502 Man Weeks $ Cost 3 1,800 8 4,800 8 4,800 12 7,200 9 5,400 1 600 3 1,800 44 26,400 MQNS 031384 -4- It ia important to note that neither Table I nor the revision take into account work done by the Spectroscopy Group (GC/Mass) duplicate analysis of samples, nor any additional method development required. The results, to date, of the biological studies were issued in progress reports distributed by Dr. Fancher. Additionally, arrangements were made to have Bio-Test store tissue samples in Chicago until they can be analyzed. db Attachment E. S. Tucker HONS 031385 'I. Residue Determinations ' TABLE I Charges A. Biological Residues (Bio-Test) 1. Rat Tissue Study 2. Chicken Toxicity, Reproduction and Meat and Egg Residue Study 3. Mallard Duck Study 4. Two Year Chronic Toxicity in Rats 5. Pish Toxicity Study Reg. Reg. Reg. Reg. Reg. 3. Samples 160 360 360 400 60+ i';T4F' Man Weeks 12 27 27 30 9 15? B. Environmental Residues 1. Outside a. Lake Michigan HARP Study b. Detergents & Components Reg. 12+MD Inorg. 20+MD 2 3 2. Monsanto a. Anniston - Water/ Sediment/Fish b. WGK - Water/Sediment c. Pensacola - Water/ Sediment/Fish d. Ruabon - Sediment e. Florida - Soil Poison Test Samples (Soil) Mfg. Mfg. Reg. Reg. Reg. II. Special Studies A. Ultraviolet Degradation of PCBs, et al Reg. B. Water Extractability of PCBs from Painted Surface Reg. C. Characterization of Competitive Reg. PCB Products D. Analysis of Auto Exhausts for PCBs Reg. E. Analysis of Pesticides for PCBs Reg. P. Determination of the Quarter Reg. Phenyl Content of Aroclor 54S0 14 in hand 13 in hand 12 in hand 4 in hand 45 X 2 165 1 1 1 1 5 14 MD 4 MD 3 MD 1 MD 1 MD 3 MD 4 - 16 Anal. Cost ( 7,200 16,200 16,200 18,000 5,400 63,606 1,200 1,800 600 600 600 600 3,000 8,400 2,400 1,800 600 600 1,800 2,400 9,600 TOTALS! Assumption - 3 hr./sample $15/hr. - $600/wk. MD - Method Development 1505 135 81,000 HONS 031386