Document npk3bBxjaekzqNenb3Z9DZom6

TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not given.The testsample isFC-206CE (5/23/91).It'psuritywas notsufficiengcyharacterizedt,hough currentinformationindicatesitisa mixtureof0.92% PFOS, 15% diethylene glycolbutylether,76.09% water,4% urea,1.28% Sultonefoamer,1.55% sodium octylsulfate,1.05% polyoxyethylenemonooctylphenylether,0.06% sodium laurylsulfatea,nd 0.05% tolytriazole.. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impuilties.Data may not accurately reflectthe toxicityof the tluorochemical component of the testsample. METHOD: Method: OECD 201 and EPA-600/9-78-18 Type: Acute Static GLP: No Year study performed: 1991 Species: Selenastrumcapricomutum Source: Originalloybtainedfrom the CultureCollectioantthe Universitoyf Texas atAustin,maintainedinculturemedium atEnviroSystems,Hampton, NH. Element basis: Algal cellcounts (cells/ml). Exposure period: 96 hours StatisticalMethods: Performed using standard statisticatlechniques (Stephan,1983).EC5o values were calculatedusingthe binomialor moving average method. The NOEC was calculatedusingDunneft'stest. Analyticalmonitoring: pH and temperature. Test Conditions: Algal NutrientMedium: Nutrientmedium per U.S. EPA, 1978 prepared in deionizedwater. This nutrientmedium providedallmineralnutrientesssential foralgalgrowth and alsoserved as the diluentforallalgaloperations.The pH ofthissyntheticalgalmedium was -8.0. Stock and testsolutionpreparation: A 1000 mg/L primarystocksolution was prepared inalgalmedium. Aliquotswere added directltyo algalmedium intestvesselsto createtestsolutions. Exposurevessels:250 mL Erlenmeyercsontainin1g00 mL testsolution and capped withinvertedglass beakers. Agitation: Continuous rotaryshaker,rateof rotationot given. Number of replicates:3 Initiaclellloading: 1.0 X 104 cells/mL Number of concentrations: fiveplus a blankcontrol Lighting: Continuous lightinagt50 uEs-1M-2 using cool-whitefluorescent lights. Water Chemistry: pH range (0-96 hours): 8.2 - 9.9 (controlexposure) 7.9- 8.3(1000 mg/L exposure) Test temperature range (0-96hours): 23.0- 23.4'C (incubator) RESULTS Nominal concentrations: Bk control,150, 250, 400, 600, and 1000 mg/L Algal Growth Response at96 Hours Nominal Conc., Avg. CellCount Avg. Specific % Change(l) mg/L (cells/mL) Growth Rate Bk. Control 479,000 0.040 0 150 718,000 0.045 -12 250 415,000 -0.039 2 400 242,000 0.033 17 600 0 0.000 100 1000 0 0.000 100 (1)Average growth rateincontrolcompared totestconcentrationgrowth rate. Element values: 96-hour EC5o: 96-hour NOEC: 456 (400-600)mg/L 400 mg/L Element values are based on nominal concentrations. Reversibilityof Growth Inhibition:Not determined. Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplyto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS FC-206CE exhibitsa 96-hourEC5o of456 mg/L witha 95% Confidence Intervaolf400 to600 mg/L. The 96-hour No Observed EffectConcentration (NOEC) was 400 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY Reliability:Klimischranking2. This study meets the criterifaorquality testing.However, the study lacksinformationon purityofthe testsubstance and the study lacksanalyticaclonfirmationofthe amount offluorochemical proportioninthe solution. REFERENCES Test was conducted by EnviroSystems Divisionof Resource Analysts IncorporatedH,ampton, NH atthe requestofthe 3M Company, St.Paul,MN, Lab Request number J1884-2, 1991. OTHER Last changed: 6/26/00 Study Title Static &cute Toxicity of rC-206CS to the Ilga, Solonastrus capricorautum Authors Robert L. goeri Tinotby J. Ward Study completed July, 1991 Performina Laboratory UviroSysteas Division Resource Analysts Incorporated One Lafayette Road laaptan, New Hampshire -03842 page I, ot 15@- 1. tummy The &cut* capricorautum, is toxicity of FC-206CB to the alga, Solonestrun described in this final report. The test was conducted for 3H Company for 96 bourn during July 9 to July 12, 1991, at the EnviroSystans Division of Resource Lualysts, Inc. in Hampton. New Haspsbir*. It was conducted by Jo,nn Nagazu, Peter Kowalski, Zllen Stanford, Robert Doori, and Timothy Ward. The test was concentrations of test temperature of 23.20C. with sterile dsionized Light was adjust*d to performed under static conditions with give substance and a dilution water control at a mean The dilution water was synthetic media prepared water. flasks were incubated on a rotary shaker. 50 ulin/sec/as with a photoperiodof 24 hours light and 0 hours dark. Nominal concentrations of FC-206CE were: 0 mg/L (control), 150 mg/L, 250 mg/L. 400 mg/L, 600 ag/L, and 1,000 mg/L. Nominal concentrations war* used for all calculations. Alga* used in the test was from an in-house culture that was maintained under test conditions. Exposure of algae to the test substance resulted in a 72 hour EC50 of 418 mg/L FC-206CE and a no observed effect concentration (NOEC) of 150 mg/L. The 96 hour EC50 and NOSC are 456 mg/L and 400 mg/L, respectively. P ag a 2 of 15 bwkfm M*ft W- Wo"w d MLLFM ii. TULII OF CONTUTS SSUION: I. Swksary ii. Table of Contents III. Index of Tables IV. Introduction V. Methods and Materials Vi. Results vir. References &pmdix A. Water Quality Data from Toxicity Test PKGR 2 3 4 5 5 8 12 13 .fir, -k Fag 9-3,of ROOM" kb"" d Ill. mzx Of TLBLES Table 1. Cbenical characterization of a representative sample of deionized water used to prepare test media for toxicitytest Table 2. Growth data from toxicity test Table 3. Percent change from control and average specific growth rat* from toxicity test Table 4. Nedian effective concentrations (IC50s) fro& toxicitytest Table A.l. Temperature measured during toxicity test Table &.2. pE measured during toxicity test PIGE 6 9 10 11 14 is 7 txmN*Mk kr-bbddk3idyMUPM IT. DMODUCTION This study was sponsored by 3N Company, St. Paul, Minnesota. The objective of the study was to determine the acute toxicity of FC-206CZ to frosbvater alga. The report contains sections that describe the methods :nd materials employed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test. TEST SUBSTANCE: V. K M ODS &ND MATERIALS FC-206CE (EnviroSysteas Sample Number 2936A) was delivered to ZnviroSystons on May 23, 1991. It was contained in a 40 oz. plastic bottle that was labelled with the following information: "JlSS4-2, FC-206CE". FC-206CE (a yellow liquid) was supplied by 3H Coapany, 935 Bush &venue, St. Paul, Minnesota. Prior to use the test material was stored in the dark at room temperature. A reserve sample (approximately 1.0 gram) will be retained at ZnviroSystans for a minimum of 10 years. DILUTION WATER: Water used for acclimationof test organisms and for all toxicity testing was sterile enriched media (U.S. EPA, 1978) with a pR of approximately B.O. Results of chemical analysis of a representative Sample of deionized water used to prepare the media are presented in Table 1. TEST ORGANISM: klgae used for the test was from a culture originally procured from the Culture Collection of Algae at the University of Texas at Austin and delivered to Ravirosystess on May 14, 1991. The identity of the culture was verified using an appropriate taxonomic key. , The culture was transferred to sterile enriched media identicalto media used for this test and maintained at test conditions. TONCITT TESTING: The definitive toxicity test, was performed during July 9 to 12,, 1991. It is based an procedures of the OECD (1934). The test was conducted at a target temperature of 22 :k20C with five concentrations of test substance and a dilution water control. k 1,000 mg/L stock solution was prepared in dilution water. The stock solution was added directly to dilution water contained in the test vessels without the use. of a solvent. Nominal concentrations of the' test material were: C no/b (control),150 mg/L, 250 no/L, 400 mg/L. 600 fBg/L. and 1,000 mg/L. page 5 of 15 ResourceArK*ift kv. &jbddby d KMLLEIM Table 1. Chemical characterization of a representative xaapl* of dsionizedwaterused to prepare test media for toxicity test Parameter organocblorino pesticides Unit of Measurement ug/L Reporting Limit 2 Organophosphorus ug/L 0.5 pesticides Polychlorinated ug/L 0.5 biphonyls Not*: ND not detected at or above the reporting limit. Measured Value ND ND ND 6 of,'I lbddby Algg* was randomly and equally distribited among three replicatesof each treatmentat the rate of lo,ooocalls/al. The test was perfora*d in 250 mi glassgrlena*yerflasksthat contained100 al Of test solution. Th* flaskb were capped with inverted glass beakers. T*st vessels were randomlyarrangedon a rotaryshakerin an incubator during the 96 hour test (a randoa numbers table was used to select the location of each vessel). A 24 hour light and 0 hour dark photoperiod was maintained with cool-white fluorescent lights that provided a light intensity of 50 uZs-lir2. The nuaber of algal cells/al in each test vessel was dateraintd visually every 24 hours by means of direct microscopiccounts with a bonacytometer. The pH (Backman model pal 51 aster) was determined in each test flask at the beginning and end of the test, and temperature (ASTH mercury thermometer) was measured and recorded daily in the incubator. STJLTISTICAL EMODS: R*sults of the toxicity test were interpreted by standard tatistical techniques (Stephan, 1983). The binomial or moving average :otbod was used by the author to calculate SC50 values using nominal concentrations of test substance. The IPOZC was calculated using Dunnott's test, which includes a parametric one-way analysis of variance (knovi). The average specific growth rate was calculated as the natural log of the number of calls per al at 24, 48, 72, or 96 hours aimus the natural log of the number of calls per al at 0 hours divided by the exposure period. The percent change from control is calculated by subtracting the masple average specific growth rate from the control average specific growth rate, dividing that value by the average specific growth rate in the control and multiplying that value by 100. The percent change from control was used to compute ZCSO and NOEC values. 7.@ 7@' ?ago 7 of 15 Ti. I&SULTS Biological and water quality data generated by the acute toxicity test are presented in Table 2 and kppendix Jk,r*spectively. and tb* percent of control and average specific growth rat* information is presented in Table 3. The 24. 48, 72, and 96 hour =50s for algae exposed to FC-206CI are presented in ?able 4. The 72 hour SCSO is 418 mg/L, and the 72 hour NOEC is 150 mg/L FC-206CE. The 96 hour SCSO is 456 mg/L and the 96 hour NOSC is 400 mg/L. z page 8 of 15 ArcMk bwr- d Table 2. Growthdata froa toxicitytest Noainal Concentration (so/L) rep. 0.0 1 (control) 2 3 mean iso 1 2 3 mean 250 1 2 3 aean 400 1 2 3 aean 600 1 2 3 R*an 1,000 1 2 -3 mean Nuabor of C*Ils per Milliliterx 103 (hour) 0 24 48 12 96 10 12 120 10 is 106 10 is 136 10 16 121 10 is 36 10 30 39 10 12 26 10 20 33 10 20 25 10 12 20 10 12 15 10 15 20 10 14 25 10 24 10 10 14 30 10 17 22 10 20 0 10 26 0 10 22 0 10 23 0 10 is 10 10 16 10 io 12 10 15 144 372 213 450 196 615 186 479 104 620 130 750 105 785 113 718 90 515 65 415 85 315 so 415 45 200 70 260 55 26S 57 242 0 0 0 0 0 0 0 0 0 0 0 0 o @O :-:@0 Page 9 of 15 Table 3. Percent change fro& control and average specific growth rate from toxicitytest Nominal Concentratioik of Test Substance (mg/L) Iverage specific growth rate 24hr 48hr 72br 96br 0.0 (control) 150 250 400 600 1,000 0.020 0.052 0.041 0.040 0.029 0.025 0.034 0.045 0.017 0.015 0.029 0.039 0.022 0.017 0.024 0.033 0.035 0.000 0.000 0.000 0.017 -0.007 0.000 0.000 Percent change from control 24hr 48br 72hr 96br 0 0 0 0 -45 52 17 -12 is 71 29 2 -10 67 41 17 -75 100 100 100 is 86 100 100 page 10 of IS RawuFm A-Olyftkr-ktddkvyd MTLFORE Table 4. Modian effective concentrations (zcsos) from toxicity test Exposure period zcso 95 percent confidence limits Calculation method 24 hours 48 hours 72 hours 96 hours )1,000 mg/L 150 mg/L 418 mg/L 456 na/L 250 - 600 no/L 400 - 600 no/L Binoaial/Nonlinear Interpolation Binonial/Nonlinear Interpolation Page 11 of 15. RO-im Alt*^ lnr&-AtxdkddOM VIZ.kirnnczs OZCD. 1984. Guidelines for Testing of Cbesicals. Section 2: Iffects on Biotic Systems. Notbod 201, Alga, Growtb Inbibition Test. Adopted June 4, 1984. Stapban, C.E. 1983. Computer program for calculation of LC50 values. Personal communication. U.S. EPA. 1973. The Solonastrus capricorautua bottle Test. EPA-600/9-78-018. July, 1978. Printz Algal Assay rag* la of ls.,. ippendix k. VKTZR ITT DATK nom TOXICITY_TEST Table A.I. Teaperaturemeasured during toxicitytest Day of Zxposure 0 1 2 3 4 Teup*rature of locubator,OC 23.2 23.0 23.0 23.4 23.2 Page 14@ 0f is . .. . Tabl* A.2. PH measuredduring toxicity test mosinal concentration (ag/L) 0 (control) 150 250 400 600 i,ooo Replicate 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 @2 3 initial 8.2 8.2 9.2 8.2 8.2 8.2 8.2 8.2 8.2 8.2 8.2 8.3 8.3 8.3 8.2 8.3 8.3 8.3 final 9.8 9.8 9.9 9.1 9.1 9.1 9.1 8.7 9.1 8.8 8.7 8.6 9.1 9.2 8.3 7.9 8.0 8.0 -@,7pigil@'ot IL5 d