Document nmxervDd8LJzL6O93k9bzVzw

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFC-602. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M producbon lotnumber was 501. The testsample isFG602-X . Currentinformationindicatesitisa mixtureof1% PFOS, 12% diethyleneglycolbutylether,61.05% water,20% ethyleneglycol,2% Sultone foanier,1% sodium octylsulfate1,% sodium decylsulfate1,% magnesium sulfate0,.7% xanthan gum, 0.1% starch,0.1% n-(3-chloroallyl)hexaminium chloride,and 0.05% benzotriazole. The followingsummary appliesto a mixture with incompletely charactedzed concentrations of impuritfas.Data may not accurately retlectthe toxicityof the tluorochemical component of the testsampie. METHOD: Method: OECD Method 203 and U.S. EPA Method EG-9 Type: Acute static GLP: No Year completed: 1988 Species: Pimephalespromelas Supplier:Kurtz'sFishHatchery,Elverson,PA. Analyticalmonitoring: Dissolvedoxygen,pH, and temperature. Exposure period:96-hours Statisticamlethods: NotapplicableL.C50valuesnotcalculatebdased on study results. Test fishage: Juveniles Length and weight: Average length 27 mm Average weight 0.15 g (wet) Loading: 0.3g/L Pretreatment:None. Test conditions: Dilutionwater: Carbon filterweedllwater Dilutionwater chemistry: Hardness: 240 rng/Las CaC03 Alkalinity: 220 mg/L as CaC03 Conductivity: 500 pmhos/cm Lighting:Not given. Stock and testsolutionpreparation:The testmaterialwas added directly tothe dilutonwateron a weight/volumebasis. Concentrations dosing rate:Once StabiliotfythetestchemicalsolutionsN:otnoted Exposurevessels:4 L Pyrexglassbeakerscontaini3ngL oftestsolution. Number ofreplicate2s: Number offishperreplicate6: Number ofconcentrationfsi:vpelusa blankcontrol Waterchemistryduringthestudy: pH range(0-96hours): 8.0- 8.2(contreoxlposure) 7.3- 8.2(1000 mg/L exposure) Temperature range (0-96hours): 20-21 OC Dissolved oxygen range (0-96hours): 7.6- 9.2mg/L (controelxposure) 1.6 - 9.2rng/L(1000 mg/L exposure) RESULTS Nominal concentrations: Bk control,100, 180, 320, 560, and 1000 nig/L. Element values: 96-hour LC5o = >1000 rng/L Element valuesbased on nominalconcentrations Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-602 96-hourLC50 forfatheadminnow was determined tobe >1000 mg/L inboth replicatest,he highestconcentratiotnested.Mortalitoybserved was -17%. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets the criterifaorqualitytesting. However, sample puritywas not propedy characterizedand thestudylacks analyticaclonfirmationoftheamount offluorochemicalproportioninthe solution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Fl 151, 1988. OTHER Last changed: 6/26/00 r-,,4vii,(umcNIALLAIJURAIURY LR FISH TOXICITY DATA SHEET DEFINITIVE- (FULL--SCALE TEST) TOE TEST AVG. TEW..24- OPZMIIW I Wr.-.0 IJR Ae,!-L--SicL4'0-CHEMICAL TESTED FC- /,C,;-k)<JLg@,+Ltjaif-r ATC I tillAIA,' SOMCE Kv,r%z's Fis,%,N%ciw-hery L'i%x-rce)n PA DATE M']Q- Pit,@Wr--TC- I BATCH NO. 13-9-ramq AVG. SIZE v? - 7 cyn No. OF oGRANi.%is PER mmimENr-4. LA)ADING FACI'04--.Q I 3 &@T. FISH DISEASE- TEST VESSEL: -@-zitel-- -- Aockver- -. TEST SOLUTION VOUM TIME STARTEQ-LLl3jC-.@@. DATE STARTED -'i--@I- 8 v - DATECWLEM DILUTION Carhnn-fil-tered wt-11--wate(rTemp.--PI, OC, D.O.'?',-1,ma/I pH LCSO(95% Confidence Limitsl- @@ JLnt) L Tank No. Initial Conc. mg/L Total Amo un Added -T Control 1-1 loo 300 Igo SY0 1-3 3,2o cl6 0. 5-to )-S-/ve,'3O00 4 Total Nc Dead TS pli 4 8 EIO s 0. Total No. pH ID.0. /L Dead hgll co 7,6 0 710 C, 9.0t-6 0 9.14.g o q.o.,59 0 @,-3 0 @.o3,s C,> 9.r->16,;Z 0 t;,@ 72 otal No Dead urs 96 rs FtD' @-HD-.O. otal mg/L To Dead No. piki.0. L R.0 gD .077,,4CA 7,916 0 7.7 7,9@,c) o 0 7,1 o 0 --D 7.ti2,IAC) C, 7.7q1 7. q 7. 7.9 L Test Temp. OC Initials & Date < CONWS: +Tna%Vv-uAtLIi.A-telavi4cil Sub Tw% SWZM)A@Y WFE@IS (=iER nM MORTALITY) LanK o. Symptoms or Reactions 24-Hr. 2 rAi.4cL;, U) 1+4,1 w ti4er. -iotai t%mer AneEtea 48-Hr. 72-Hr. 96-Hr. @u 0 Test methodology confonas with the following two test protocols: (1) OECD 0203 "Fish, Acute Toxicity Test". (2) US-EPA IBG-9 "Fish Acute Toxicitv Test". L.4VIAUI'tvl='41AL.,L 'MKAIURY FISH TOXICIIY DATA SHEET DEFINITIVE- (FULL-SCALE TESR) I,gF 1,SI- IWE TESRIt,-,4RAciig..Si(-tlClHcEMICALTFSI'El)ff--@tcioe-l@6LxieeLiAeT(P-?fL,,@, Wr 6-o I TEST ORGANISW.FO-*\nen el -Iki Int, to SOURRE kuri2 s I-Ivc, RcAere Pg -'yDATE REC'I) BATCH .40. AVG. Vr. ':@-gramq AVG. SIZE -7 NO. OF OGRANI.%ISPER ntEA7MENr--L- LOADING ().J gil, FISH DISEASE TEST VESSEL: /-,'+.er- 13eo-kev- @ TEST SOUITION VOLUME T. TIM DILUTION TTER ANALYST.- DATE STAPTED -3 DATE COWLETM Carhnn-f iItprpd wpi I water (Temp. or-,,D.O. pHA P,- &zt-C@@LCSO(95% Confidence Lindtsl- L Tank InitialT'ioCot)atCla)i oun T 24 jours 48 Hou s 72 Hours 96 Hours Conc. Ammo Total Nc. i .0. Total No. pH]D.O. I otal No. pthDi. Total No. pH mg/L Added Dead PliPi/L Dead rg/L Dead mg/L Dead L Control /&o 3c)c) 0 2 ),So 540 0 ?-'D' t-*1.Jo,@ (760. C) i 6,qID) 0 o o C) '3000 o cl 0 .171 cl 0 9.16, o -c C) 9,o 9.o 17, I 0 S.,c 7,17-:1@ 0 0, 7,914.101 C) 7.6,7.0 (D 7q 1 So 7.-7S.1 7,7 7,q3.1 7--1 7311.7 Test Temp. OC Initials & Date Ca44WS: 3 'o SECONDAU e',+06 r%t-, eliieco It)(V) 03ti WFE 'CfS(anM UMMORT'ALITY) q -<.Ag3 cippor@,n4ritglisei'c6u1i,+eL-Lua4er L:@ No. Symptoms or Reactions 24-Hr. 'rotalM=er ArrecrezI 48-Hr. 72-Hr. 96-Hr. Test methodology conform with the following two test protocols: (1) OECD 1203 'Tish, Acute Toxicity Test". (2)US-EPA IBG-9 "FishAcute ToxicityTest". 03/24/1988 ENVIRONMENTAL LABORATORY FINAL REPORT 1 LAB REQUEST NO. F1286 REQUESTOR NAME: RR ROBIDEAU/SAB DEPARTMENT: 0222 PROJECT NO: 06 DATE RECEIVED: 03/02/1988 DESC: CARBON-FILTERED WELL WATER - FEB. 1988 CONTRACT LAB: PACE CONTRACT LAB COST: 144 3M E-L HOURS: 1 EXP COMP DATE: 03/23/1988 DATE COMPLETED: 03/23/1988 ORIGINAL SOURCE OF, WELL WATER, 3M WELL 02, ST. PAUL, MN. ------------------------------------------------------------------------------ SAMPLE DATE CODE DESCRIPTION RESULT MIN DET LIMIT ------------------------------------------------------------------------------ 1 03/02/88 ROOM 4 WELL WATER *COD <5 MG/L 5 *SPECIFIC C014DUCTANCE 500 UMHOS/CM *AMMONIA NITROGEN - as N <0.1 uin-L:t H 6 UNIT 0. 1 *SOLUBLE FLUORIDE (PROBE) *STANDARD PLATE COUNT 17 COL/ML *TOTAL DISSOLVED SOLIDS *TOTAL ALKALINITY (PH 4.5) 280 MG/L 220 MG/L *TOTAL COLIFORM *TOTAL HARDNESS <1 COL/100ML 1 240 MG/L ------TOTAL RESIDUAL CHLORINE <0.02 MG/L *TOTAL SOLIDS 280 MG/L *TOTAL SUSPENDED SOLIDS <1 MG/L 1 * = CONTRACT LAB SUBMITTED BY: DATED: