Document nmkJ5M7ev040g0ko470Q706E2

y^afejb^aaA,;. &U* iafriir.iMItt*--.wttw -*-'-*- mao pw o j t c t stsoturr leauo Datet August 1967 PBOJDCT WO. 18-126 T7TLS X. Effect of lead oa tUia* cultura of the nervous ayitaa. XV. Coaparstlve study of the pottarn of abeorp* tloo of land, tin, mercury and coppar by nervous tissue is culture. 2. The Influence of lead nod otbar heavy artels oo tba eynthesle aod tum-ovtr of KkA la >!! calls. mnoug xtcrmcAnow noo* OomUTTOR Tbs University of Viseoasis CTwtm ocamucr seas CtxnATIVI Bcmsg (to July Jl, 1967) soos 19^P ncPoeis q> o o s t mc t mat $19,000 1966 A17TH0PIZAT10M lyo7 4 Prior Authorttattoos Obcaaaltted 1966 Proposad Authorisation Ubdesigna'.ed be search Autborltatloa 1966 Proposed or Coe*, met Pries $7,900 IkOM $19,006 ($7,500 iu j o ) $7,500 m) HtWTT ATTUfATlOSB Direct to gasolias additive, sod indirectly to aost otbar usee of lead. CMKrT'VI To !nvaii(ais hutochenically the call* which aieo.'b tin, mercury and copper as compared to laad to nervous tissue Is culture, and to investigate tba influence of baavy satsls oo tba synthesis of MA 1s treated calls. wstAfCH wvraf I"! Current sTtu Tba present lbvet ig*tor, Dr. frobfcowict, bas sovad to tba Ufalverslly of Vise coats where ana ha* sad* arrangenrete to continue bar loves* ligation* Tbe proposed wort will follow directly that which w spassored at Coluahla Uhlvrlty as Lg-117. 2. Project at Research To teat the theory that the toslelty of a baavy aatal la related to it* ability to penatrata in*id* of tba call, a comparison of tba morphology of nervous system culture* exposed to a eerie* of satsls with tbs histoebealcal determine!loo of tba aatal*' absorption by oervou* tissue la vitro will be made. Tba Influence of bear/ aatala oo tba Synthesis of NIA la treated calls will be studied. Such Information will Increase our understanding of aolscular event* resulting in cell injury and death due to baavy aatala laical* cation and increase our under*landing of th* regulatory aachanlta vltblo tbo living calls and organs. J. Mport Iu*4 a. Fo ,'port * neats b. K^mT~i . kffeet of teal on it*ue culture of the nerve*,* ayatoa. Tv. t\j*inraUva study of th* jettem of absorption of lead, tin, nercury end copper by nervou* tl**u* In culture. ?. Tba Influence of leal and other naavy aetalt on the *yhthe*l* and lurn treer of IWA lb aaxaa.iao call*, (dated retruary 71, 1967) / PI I.- P>.miWBi'i.P .n yiW.wpgiWirlJii- fUiJNin,U. .J.iI|WWjp|g<|jp^BppiB^p^pi^ LIA12327 n i if'iiirit'iii VihifTlalftilinfiMflUifii U*U6 zuw rnoncT k j n o u u *** two e. Wort Btate--ct mm acmorwBgww 1. ' Pilot h-cxiaction - sot applicable 2. Coniwir Evaluation aot applicable 3. Licensing Activities * sot applicable a. hutu aot applicable $. Publication* a. U1K BOM b. DrvelopMOt aeaoclatloM mm k o ip c t c ucunm 1. Mu-act Ooale - Maintain world aartet. 2. Applications *ad Mrtet Wolfe * Oat at ftaollM additive. 3- Blio ef Msrtct Dcfcndlne - 2)0,000 tOM of lead la U.B. sad $1,000 too* la turope. A. Competitive Threat - Chance refining technique* to ellaloate lead additive*.