Document nmkDKNXNRx9ONg1vbwBjQr62z
:
AR 226 -- 0255
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
JESTSUBSTANCE
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number is not noted. The test sample is FC-603EF. Current information indicates itis a mixture of 1.30% PFOS, 10% diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6% propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3Chioroallyijhexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0% triethanolamine, and 0.05% tolyl triazole. The following summary applies a mixture with incompletely crehfalreaccttteorxiizceitdycoofnctehentfrlautoirooncshoefmiicmaplurciotimepso. nDeanttaomfatyhetneotstacscaumrpaltee.ly
METHOD
Method: OECD Guideline 209
Test type: Acute static
GLP: No
Year Completed: 1996
Analytical monitoring: Dissolved oxygen, pH, and temperature.
Statistical methods: Results were determined by calculation of the %
inhibition in respiration rate. Test organism source: Activated
sludge
mixed
liquor
collected
from
the
Metro Waste Water Treatment Plant, St. Paul, MN.
Test condition:
Dilution water: Millipore Mill-QTM water
Synthetic sewage: per OECD method
Reference and test solution preparation: A stock solution of the
reference substance, 3,5-dichlorophenol, was prepared by dissolving
w5a0t0erm,gthienn1b0rmoLug1hNt tNoatOhHe,podiinltutoefditnoci3pi0emntLpwrietchipMiitlaltiipoonrewiMtihl1iN-QHTMzSO4
awnads dmieluatseudrteod1tLowbieth7.M2i.llTie-sQtTM swuatbesrt.anTcheewpaHs aofddtheed rdeifreecrtelnycetostohleution
test vessels.
Testvessels: Not given.
--
Number of concentrations: three plus 1 reference substance and
blank control
Temperature: 19.4 --20.6 C
Total suspended solids and pH on dayof testing: 2.96 g/L (TSS in
final: 1.18 g/L), initial pH 7.3 and final pH 7.6.
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Element Basis: Respiration inhibition as determined by oxygen consumption. Remarks: Values corrected to 20C for calculations.
RmESmUmLTmSsmsmmim------------------------------------
Nominal concentrations: Blank control, 10, 100, and 1000 mg/L. The blank control and 1000 mg/L exposure concentration were evaluated in duplicate. Reference substance at 10 mg/L. Element values: 30-minute EC50 = >1000 mg/L
3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
conclusions
The test substance 3-hour ECso for activated sludge respiration inhibition was determined to be >1000 mg/L, the highest concentration tested. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
De ATeA U eA eT ------------------------------------
Reliability: Kiimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution.
|e REEEc RENCs ES mrmmm----------
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number P2581, 1996.
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Last changed: 6/26/00
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