Document nmg59Qd99z54bbJoro5VXMY6G

Srk JLA6 - 0 2 ? * f BIODEGRADATION (MANOMETRIC RESPIROMETRY - AEROBIC) TEST SUBSTANCE _________________________________________ Identity: A mixture containing periluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-3041. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-3041. Current information indicates it is a mixture of 6% PFOS, 36% diethylene glycol butyl ether, 36.05% water, 12% Sultone foamer, 9% sodium octyl sulfate, 0.55% sodium lauryl sulfate, and 0.4% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the biodegradability o f the fluorochemicai component o f the test sample. METHOD____________________________________________________ Method: Manometric Respirometry Test Test type: Ready biodegradability GLP: No Year Completed: 1993 Analytical m onitoring: Oxygen consumption determined by measurement of the amount of oxygen generated electrolytically to maintain a constant gas volume in the respiration flask. Depletion of dissolved oxygen was registered automatically. Amount of oxygen taken up is given (after comparison with the blank inoculum) as a percentage of the chemical oxygen demand. S tatistical m ethods: Results were determined by calculation of the percentage biodegradation. Inoculum source: Activated sludge collected from a community sewage plant (Schmallenberg, Germany) that was fed almost exclusively with residential wastewater. Test condition: D ilution water: Not noted. Mineral nutrient m edium : Mineral Basal Medium Stock and te st solutio n preparation: Solutions of the test and reference materials were prepared at 100 mg/L with mineral basal medium. Test vessels: Closed flasks kept at a constant temperature. Each vessel is connected gastight to the pressure regulator corresponding to it. Incubation C onditions: Per manometric method Temperature: 2 2 2 C A gitation: Continuous stirring Number o f concentrations: Test sample, test sample inhibited, test sample plus aniline, aniline (reference substance) and blank inoculum, all in duplicate. 002875 Inoculum condition on test initiation: Initial solids 3 g/L, 30 mg solid matter/L. Element Basis: Biological degradation by means of oxygen consumption, mg (V i le s t substance flask conditions: pH: Not noted. D.O.: Automatically measured. R E S U L T S _______________________________________________ Nominal concentrations: Blank control, test material at 100 mg/L, and aniline (reference substance) at 100 mg/L. Element values: 14-day Degradation with Test Material = 93.8% based on average of two replicates minus the blank. 14-day Degradation with Test Material plus Aniline = 88% based on average of two replicates minus the blank. 14-day Degradation with Test Material Inhibited = 8.5% based on average of two replicates minus the blank. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test material % degradation based on the mean of two replicates minus the oxygen consumption of the blank control was 93.8% after 14 days. The reference substance obtained 82% degradation by 14 days, thus the validation criterion of the test was fulfilled (>60% after 14 days). The test was terminated on day 14 since the validity criterion of the test was fulfilled and the oxygen uptake reached a plateau. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking 3. Lacks specific methodology used and documentation. Sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ This study was conducted by Fraunhofer Institute for Environmental Chemistry and Ecotoxicology, D-57377 Schmallenberg, Germany at the request of the 002876 Federal Bureau for Material Technology and Acquisitions, Germany, Grant No.: E/B31E/P0078/N52275, 1993. OTHER______________________________________________________ Last changed: 6/26/00 002877 tz C^'hoLfl FINAL REPORT ECO-TOXICOLOGICAL EFFECT OF FLUORO-SURFACTANTS G rant No.: E /B 31E /P 0078/N 5275 C lient Bundesamt fr Werictechntk und Beschaffung, Koblenz (Federal Bureau for M aterial Technology and Acquisition! Investigating Institute: Fraunhofer Institute for Environmental Chemistry and Eco-toxicology D-57377 Schmallenberg Institute M anagen Prof. Dr. W . Klein Project leaden Dr. R. Debus Project authors: Dr. A. Fliedner Dr. K. Hund DipL BioL R. Niemann Dr. A. Remde December 1993 Translator. Andrea-Ingrid Sdradder. 715-549-5734 (Gennan #1453/1995) 002878 Fraunhofer Institute for Environmental Chemistry and Eco-toxicology D-57377 Schmallenberg Investigation for the Eco-toxicological Evaluation of the Fluoro-surfactant Base FC-3041 Aerobic Dgradation Partf by Dr. A. Remde Translator. Andrea-Ingrid Sdm ckte, 715-549-5734 (Gom an #1453/1995) 2 002S7S AEROBIC DEGRADATION The determination of the easy biological degradability of Base FC-3041 under aerobic conditions was done in the manometric-respirometry test (8). MATERIAL AND METHODS Test substance: Base FC-3041 (combination of fluoro-surfactants in aqueous solution; Manufacturen 3M Company Germany, Neuss). Test criterion: Measurement of the biological degradability by means of the oxygen consumption. Test m ethod: A defined volume of an inoculated mineral medium with a known concentration of the test substance (100 mg/L) is stirred in a closed flask at a constant temperature (22 'C 2). The oxygen consumption is determined through the measurement of the amount of oxygen generated electrolytically that is necessary to maintain a constant gas volume in the respiration flask. The generated carbon dioxide is absorbed into a solution of potassium hydroxide. The amount of oxygen taken up by the test substance is given (after considering the corresponding value in the inoculum blank test performed in parallel) as percentage of the CSB1 (chemical oxygen demand). Test procedure: 10 test vessels were prepared for the performance of the test: Vessel 1 and 2: Vessel 3 and 4: mineral basal medium + inoculum mineral basal medium + reference substance (100 mg/L) + inoculum (30 mg solid matter/L) ^ E n g lis h : COD. I will preserve theGerm an abbreviations since they are present in formulas and tables. Translator. Andrea-Ingrid Schneider, 715-549-5734 (German #1453/1995) 3 002880 Vessel 5 and 6: mineral basal medium + test substance (100 mg/L) + inoculum (30 mg solid matter/L) Vessel 7 and 8: mineral basal medium + reference substance (100 mg/L) + test substance (100 mg/L)+ inoculum (30 mg solid matter/L) Vessel 9 and 10: mineral basal medium + test substance (100 mg/L) + inoculum (30 mg solid matter/L) + mercury chloride solution (0.001% final concentration) The vessels were closed and placed into the respirom eter. After temperature and pressure compensation (approx. 0.5 hours), each vessel was connected gastight to the pressure regulator belonging to i t During the test period the depletion o f dissolved oxygen was registered automatically. _ The bio-degradation was determined according to the following equation: Percentage bio-degradation = - l a j} o r lnM sx 100 BSB = biochemical oxygen consumption of the test substance (mg) B = depletion of dissolved oxygen o f the basal medium with inoculum (mg) CSB = chemical oxygen consumption2 (mg) ThSB = theoretical oxygen demand (mg) a Inoculum : Active sludge from a community sewage treatment plant (Schmallenberg) which was fed almost exclusively with residential waste water, was used as inoculum. The active sludge was aerated during the transport The inoculum was adjusted with isotonic Ringer's solution to a solids content of 3 g/L and aerated. The sample collection occurred on 10/28/1993. 2in die text above, CSB was chemisher Sauerstoffbsdarf, which translates to chemical oxygen demand. In this case the same abbreviation CSB is used for chemischer SauerstoJJverbruuch which translates to chemical oxygen consumption. Translator Andrea-Ingrid Schneider, 715-549-5734 (German #1453/1995) 4 002881 A nalysis: To determine the dissolved oxygen depletion, a sapromate o f the Voith Company (Heidenheim) was used. The oxygen consumption was automatically registered as BSB/L. Reference substance: aniline (Merck, reagent grade) Test period: 10/29/1993 - 11/12/1993 RESULTS The results are represented in Tab. f l and Fig. f l. During the incubation period of 14 days, 82% of the reference substance aniline was degraded. The validity criteria of the test (degradation o f aniline >40% after 7 days and >60% after 14 days) were fulfilled. 93.8% o f the test substance Base FC-3041 was degraded in the same period of time. Since already 88.6% of the substance had degraded within 10 days counted from the day the degradation for the first tim e surpassed 10%, the substance may be categorized as easily biologically degradable. The test could be terminated on day 14 since all validity criteria o f the test had been fulfilled and a plateau was reached for the oxygen uptake. EVALUATION Based on the present results, the test substance Base FC-3041 was categorized as easily biologically degradable. Translator. Andrea-Ingrid Schneider, 715-549-5734 (German #1453/1995) 5 0028S2 Table fl: BSB and % of biological degradation o f the test substance FC-3041 and o f the reference substance aniline Start of the test: Test substance (TS): Test concentration: Chemical oxygen consumption (CSB): Reference substance (RS): Test concentration: Theoretical oxygen consumption (ThSB; NH3) Inoculum 10/29/1993 Base FC-3041 lOOmg/L 0.88 mg/mg substance Purity: - aniline lOOmg/L 2.41 mg/mg substance active sludge Day3 Inoculum Reference robstance Test substance RS + TS TS + HgCfc - BSB l ) S BSB 21 S BSB 31 S BSB 31 Z BSB 4) Sg 0 2 /1 I f 0 2 /1 X degradation | g 0 2 /1 X degradation a g 0 2 /1 Xdegradation ag 0 2 /1 1 9,5 2 14,0 3 18,0 4 20,5 5 22,5 6 24,5 7 28,0 8 28,0 9 29,5 10 30,5 11 *2,0 12 32,5 13 34,5 14 34,5 0 ,0 0 ,0 1.0 14,5 57,0 114,5 165,5 175,5 184,0 188,5 191.5 194,5 196,0 197,5 0 ,0 0 ,0 0,4 6,0 23,7 47,5 68,7 72,8 76,3 78,2 79,5 80,7 81,3 82,0 10,5 17,0 22,5 3 4 ,0 5 3 ,0 66,5 7 1 ,0 74,0 76,5 78,0 79,5 81,0 82,5 82,5 11.9 19,3 25,6 38,6 6 0 ,2 75,6 8 0 ,7 84,1 8 6 ,9 8 8 ,6 90.3 92,0 93,8 93,8 10.0 14,5 33,0 172,0 238,0 256,5 266,5 272,0 276,5 281,0 284.0 287,0 289,5 289,5 3 ,0 4 ,4 10,0 52,3 72,3 78,0 81,0 8 2 ,7 84,0 85,4 8 6 ,3 8 7 ,2 88,0 88,0 1) Inoculum control 2) Average value from two experimental runs, minus the oxygen consumption by the inoculum control 3) Average value from two experimental runs, minus the oxygen consumption by the inoculum control 4) Test substance with inoculum and HgC^ solution, average value from two experimental runs 2 ,0 3 ,0 3 ,0 4 ,0 5 ,0 5 ,0 5 ,0 6 ,0 6 ,0 7 ,0 7 ,0 7 ,0 8 ,0 8,5 3In the original only g is visible. By analogy to table g l on page g/6, the heading of this column m ust be Tag which translates as day. Translator. Andrea-Ingrid Schneider, 715-549-5734 (German #1453/1995) 6 002883 Fig. fl: Biological degradation of the test substance BASE FC-3041 and o f the reference substance aniline in the manometric-respirometry test 3 - Bast FC-3041 (TS) B - TS RS A n ilin e (RS) Translator Andrea-Ingrid Schneider, 715*549-5734 (German #1453/1995) 7 002884