Document nmZbd9gJr6ZN4MaVGvRVOrK1R

May 31, 1944 Owens-Illinois Glass Conpany Toledo, Ohio To Saranao Laboratory, Dr. Complete Study of Samples of Hydrous Calcium Silicate $250.00 Leroy 0. Gardner, M. D. Director, Saranao Laboratory 00030 - x o n I Cn February 2, 1943 we reo< red fron Ovens Illinois Glass Co., tvo f*:~ples of a new produot i tnat*?. as 'Hydrous Calolun Silicate" for as-.ay of phyalologioal hazat of inhalation of Inhaled dust. They ^ere labeled respectively ' ander Du6t* and "Rotoolone ^ust." Preliminary inspeotlon lnd: cul-tto ini flbro*is materials, t; asber*A combined petrograp! veele*1. the foll.v-ring approxlaatt ited that they were mixtures of partllatter having the characteristics of a and X-ray diffraction analysis recompositions: Quartz \y0 *TM1 * Vi "Iw RCTCC3 j2 dost petticgfaph: 20 to 30* APPROXIMATE ST7F FTC. 3 to 00 niorons. Little less than 10. Calcita 3 10 to 20 Altered (by heat*) Glas?? 10 Chryactlle (Asbestos) 25 30 Few single fibres lees than 50 ciorons More less than 20 microns, host of the single fibres 'tore than ICO alcrons. Much in unopened bundles. Hydr -teu Ca jilloats 50 -- ------ ------- ------------------------------------- ------------------------------------------------------- -------...-------------- Dlatonlte Trace -- 3AXP7 _ QUO? Quartz 7.5* 5 to 10% 6 to 120 aiorons Calcite 3 10 Altered Fibres Chryoutile 23 food 20 to 40 OlnilaT to that fron rotoolone Coarse,aatted together Hydrated Cu. Gilioate 25 to35? -- i 00030i Asbestos Litig. Saranac Lake 1989 Depos. Exhibit p.33 AHIKAL A3T.AY3 JuvUi.** livlNstad' material ac received was toe coarse to be Injected into dr.L.-.ula and had to ba reduced to proper particle 3lza. 3rluting was not feasible owing to the presence of chry90tila whose structure and irritating properties are destroyed by prolonged grinding. -e ware therefore cci-.ujiiad to reduce it by prolonged cutting with scissors, sieving through a ICO nosh screen and adjusting the proportion of fibres and partioul-.-.ts natter In the product to that of t.'-.s original epeeinane. Portions of each sanple were node up in physiological cult solution in strengths of 5 and 10,t for guinea pig injection. The pH of the rotoclone sample was 7.5 which should caaao no tissue irritation. That of the sander dust was 9 which is too alkaline to be wall tolerated. The 10? suapsnaicts were injected intraperitcnoally, u aingle doss of 2 o.c. le. 500 tag of dust. The 5? suspensions were administered directly into the lungs by tracheal catheter, 5 doses of 3.5 c.o each at intervals of 6 days, a total of 75 ag of duet. Intraaerltoneal InJeotlona of neither sample were well reoeived; 60% ot those treated with the rotoolone staple died within 2 to 3 weeks and 80< of the ones getting the sender dust were dead in 3 days. All these pigs showed acute lnflsanatory changes about assess of dead cells where large aoouBuletlone of the diet had localized. The early result could not be evaluated except for the faot that it never oocurs after the injection of quarts or asbestos alone. Bovever, there were 3 aniaala that survived for 8 nonthe, 2 reoelvlng rotoolone duet and one, sander duet. In neither of then was there any suggestion of silicosis on gross or niorosoopio examination. An asbeototlo change is not Asbestos Litig. Saranac Lake 1989 Depos. _____________________________________ Exhibit p.34 flftnQ/1 UllUov*. a i aV r >-w . . fj. w/ d. -J' * 1 ANIMAL A33AY3 CONT'D recognizable outolde of the lungs and henoe v/ould not be expactad In this group. Intratracheal in.1 motions were somewhat better tolerated parti cularly in the case of ths rotoolone sample mere there '.ere only C d-*-'.th* r. initials _.vi these occurred 3 to 10-months after com pleting the in'sctlc/.o. Of the s&nder duet animals 3 died of acute r-'-vction during the period of injection, presumably on account of the high ul salinity. One was killed 1 mouth a a*.: another 4 uontha titer completing the injections. 2oth ear.plee produced fibrosis "itliiii uuu about the terminal brcr.c ioles in all animals surviving for 1 month. or more. Inis reaction /: wae gsr.irRlly comparable-to thvt observed on injecting pure chrysotile aobectoa. Tynioal aabeetosia bodies were present in a;.all numbers and the dlati Ibutioii of `.ho leLions vi.e .typical of injection aebestosie. The only difference van a tendency for the fibrous tissue u tuore denes u.d sr..e--hs.t hyi-.iinir.ed after to 1C C/i months. This eiisct le probably attributable to the quartt In the Dirt UJ*G JL*_E EtlTS of silicotic nodular fibrosis could be discovered either within the lungs or the associated tracheobronchial lyuph nsuec. Summary: After further reduction in size the two samples of this nlxture of asbestos, wood fibre, quarts and hydrated oalclua silicate is toxic on lntraperltonaal and intratracheal injection into guinea pigs. The high alkalinity of the sandier dust probably accounts for the earlier _* deaths in the oase of ln&aparltoneal injection where a large do3e la concentrated in a looaliaad cavity and absorption le rapid. However, the deferred deaths from the rotoolone sample with a pH of o..ly 7.5 eug- i / gests sone other injurious property. Asbestos Litig. Saranac Lake 1989 Depos. Exhibit p.35 00030:. A31MAL ASSAYS C^'T'S -he chronic effects in the fev animals surviving for a m-r.th cr uore are apparently duo to the fibroau oor.ponents c? t^e d.rt :.:J. .t'-.eir nature indicates that asbestos is responsible. This Is somewhat notified either by previous treatuent of water or by the associated cuar b,,t thnrr :.u nothin su&peative of silicotic nodular fibrosis. If the hydrous cal dun Billcate has any part in tte picture such ;_rt is not recognisable. Whether aanufacture or use of this product would ever induce particle sire to the extent thnt -.`.'e have cons is outside of the -rovlnce of this study. If it should a potential hazard vould be created. How ever, study of the tvo samples suDdttcd outjeut3 trai most of the asbestos fibre is either very long cr is composed of unopened ouridles of fibre, neither one of which la readily inhalable. Leroy i. Gardner, h. 3. director, Laboratory Asbestos Litig. Saranac Lake 1989 Depos. 00030 .