Document nmYZ2a39a162ZqV7XdewKx10w

TO XIC ITY TO AQUATIC PLANTS TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FX-3103. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was not noted. The test sample is FX-3103, 3%. Current information indicates it is a mixture of 0.96% PFOS, 20% diethylene glycol butyl ether, 50.56% water, 15% ethylene glycol, 4.32% sodium octyl sulfate, 6.0% polyoxyethylene monooctylphenyl ether, 2.5% propane sultone foamer, 0.5% sodium nitrate, 0.06% sodium lauryl sulfate, and 0.1 % benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fiuorochemical component o f the test sample. METHOD:_____________________ ________ _________________________ M ethod: Not given. Type: Acute static GLP: No Y ear study performed: 1985 Species: Selenastrum capricomutum Source: Not given. Elem ent basis: Algal cell counts (cells/mL). Exposure period: 96 hours Statistical Methods: IC50values calculated using probit analysis or other regression models. A nalytical m onitoring: Not given. Test Conditions: A lgal Nutrient Medium: Not given. Stock and test solution preparation: Not given. Exposure vessels: Not given. A gitation: Not given. Num ber o f replicates: 2 Initial cell loading: 2.05 x 104 cells/mL Num ber o f concentrations: five plus a blank control Lighting: Not given. W ater Chem istry: Not given. RESULTS___________________________ Nom inal concentrations: Blank control, 100,180, 320, 560, and 1000 mg/L 002965 96-H our A lgal Growth Response Nom inal Cone. (m g/L) Control 100 180 320 560 1000 Mean C ell-C ount (cells/m L) 1,790,000 1,770,000 1,080,000 720,000 310,000 200,000 Percent Inhibition -- 1 40 60 83 89 Elem ent values: 96-hour IC50 = 236 (193 - 273) mg/L Element values are based on nominal concentrations. R eversibility o f G row th Inhibition: Not given. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS________________________________ ____________ - The test substance exhibits a 96-hour IC50 of 236 mg/L with a 95% confidence interval of 193 to 273 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ R eliability: Klimisch ranking 3. Study lacks description and records of the method followed. There is no characterization of the test substance purity, and lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number C1111-1, 1985. OTHER__________________________________ ;________________________ Last changed: 6/26/00. 002966 ENVIRONMENTAL LABORATORY FRESHWATER ALGAE GROWTH INHIBITION TEST ALGAL CELL COUNT, no./ml HEMOCYTOMETER METHOD* LR 2 1 /L . / TEST DAY. _____ __TEST tvpf _________ DATE. z J z ^ / b x - MATERIAL TESTED:. ----------------------------------- ALGAL SPECIES`i Green Algae. S^Tenastgjm CflprIfiQrnutM TREATMENT CONTROL X in lO O ^ M ' M Z -l I0 O ^ \t& S Z D w a (J( 1 -5 2 .-5 Z .-4 l o o O u q UL 1 -5 Z rS * TOTAL OF 25 FIELDS COUNTED 180 ne no./ml 1 .000,000 | , `f t P i0 0 0 MEAN + SD 1 ^ 4 0 ,0 0 0 we nu> l ,1<3O,0OO 1t'ibO i 0 0 0 l 1vT'1 O )0 0 O ho I0(p 1 ,1 0 0 ,0 0 0 1^0(^0,000 ' ,0 8 0 ,0 0 0 nz. 30 zo z~o 'IZ Q fiO Q 'IZO iO O O ^ Z jO jO O O 3 2 0 jOOO,. 3 0 0 ,0 0 0 0 0}0 0 0 Ajdo{ o o d 6 1 0 )O 0'O 1 ZO O {0O O * 0.1 m deep Hemocytoroeter* area of field 1 x 1 and 0.1 mm deep. 002967 ENVIRONMENTAL LABORATORY TABLE 1. SUMMARY OF ACUTE TOXICITY OP ____ TO FRESHWATER ALGAE (SELENASTRUM CAPRICORNUTUM)*1 * 01111-1 % Percent Inhibition in Standing Crop ^ Chemical Concentration 48-Hr. (mg/1) Based on 96-Hr. Based on Based on Cell-Count Cell-Count Cell Dry Wt. Control t ' _IQQ_ -JflP-- -32.a . t 4a leO 35 JQQQ 8R IC50 (95% Confidence Limits) mg/1*3 * Z S ip ^C S P tab .lli? Y ...9? -? .flt The Selenastrum capricornutum control cultures have met the folliowing' quality criteria - Exhibited log phase growth within hours (max. 48 hr.) - Attained a 96-hr. standing crop of UTAXirfrcella/ml (min, IQ5 cells/ml) - QtrotoHv. H & --VUTS H4\5",COOCellslvHjL )oddvvc( Zi>,soo eell^wi (1) Data were averages of two replications for each test concentration. (2) Based on cell-count (no./ml) and/or cell-dry weight (mg/1). (3) ICc0 values were calculated using Probit Analysis or other regression models of experimental data. 002968